RchiOBHm_Chr6g0312101

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0007813
Physical Location & Seq
Forward (+)
68812999 .. 68818703
5705 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28073

Sequence Viewer

Length: 2820 bp
ATGCACCCAAGAAATAATGCAGCTGCAGACCAATCTCCGACCCAAATTAATTTTCTTCTTCACCAAAAACCCGAAAACCCTCTCCCTCTCTTCAATTTCTCCTCTGTCTCCAACCCCATCTCTCACTCGCAACCCTCTTCTCCATGGTTCTCTATCCTCCGCACCGCCATTGCCAATACAGACTTGCCCCTCGGAAAGCGCGCCCACGCCCTTATGTTAACCTCAGGGGAATGCCAAGATCATTTTCTAATCAACAATCTCATCACCATGTATTCGAAATGCAGGTCGCTCACATATGCACGCCATCTGTTCGATAAAAGTCCTGACCGAGACCTGGTCACCTGGAATGCCATTCTAGCAGCCTATGCACAAGCTGGGGGCCCTGTGGTTGAGAATGTTCAAGAGGGACTCGGCCTTTTCCGGCGGCTTCGTGAGTCTGTTGTGTTCACCAGTCGGCACACTTTGGCTTCCGTTTTGAAGCTGTGTTTGCTGTCAGGGCGTGTTTGGATTTCGGAGGCTGTTCAGGGTTACGCTCTGAAAATTGGGTTGGAGTGGGATGTGTTTGTTTCGGGTACCCTGGTGAATGTGTATTGTAAACTCAGAAGAGTTAAGGAGGCTCGGGCTTTGTTTGATGGGATGCTGGAACGGGATGTTGTGTTGTGGAACACAATGCTGAAGGCATATGTGGAAATGGGTCTTCAGGAAGAAGCACTTGCTCTTTTCTCCAAGTTTCATCAAAGTGGATTAGGTCCTGACCATGTTAGTGTGCGTTGTGTTCTTAGTGGGATTCACAAAGTTGATTATGTTGAAGGCAAGAGGTACATGGAGGAGGTTCAAGCATATGCAGCAAAGCTGGGGGAGTTTATTATAACTAATGTGGATGATGATGCCCATCCAACGAGTTTATGTGATCCTACTACTAATTCCGACATATTTCTGTGGAACAAGAAGTTGTCTAAGTATATCCGAGCTGGTGAAAATTGGGCTGCAATAGATTGCTTTAGAAATATGATTATATCGAAAGTTGGATATGACAAGGTGACATTAGTTATCATTCTATCTGCGATTGTGGACACAAATAATTTGGAGGTGGGGAAACAGATTCATGGTGCTGCTTTGAAATCAGATTTTGATTCTGTTCTTTTGGTAGCAAATAGCCTTATCAACATGTATTCAAAGGCTCGCTGTGTTCACTTTGCGAGAGAAGTATTCAATCACATGAAAGAAGTGGACCTAGTTTCATGGAACTCGATGATATCATGTTATGTGCAAAGCGGTTCAGGAGAGGAATCAGTAAACCTATTTATTGGTTTATTACGGGATGGTCTAAGACCTGATCAATTTACAATTGCAAGTATTTTAAGGGCTTGCTCCTCTCTTCAAGAAGGGCTGCATCTCAGTAAACAGGTTCATGTTCATGCAATAAAAACTGGTATTGCTGCTGACAGATTTGTTTCAACAGCTCTTATTGATGTTTATTCCAGAAGTGGAAAGATGGAGGAAGTGGAGCCCCTTTTAGAAAACAAAGTTAATTTCGATCTGCCATCTTGGAATGCATTGATGTTTGGGTACATAAAGAGTAATGACGGTCACAAAGCACTACAACTTATGAGGATGATTCATGAAGGTGGAAAGAGGGGAGACGAGATTACTTTGGCAACTGTTGCTAAGGCCACTAGTTGCCTGGTAGCGTTGGTACCGGGGAAGCAAATTCATGCTCATGTCATAAAAACAGGGTATTCAAAGGACTTATATGTTAACAGCAGTATTCTAGATATGTACATAAAATGTGGAGACATGGGAAGTGCGCACACAGTTTTCAATGACATTCCTGCACCTGATGATGTTGCGTGGACAACTCTAATCTCAGGATGTCTAGAAAATGGAGATGAAGGGCGCTCAGTGTCTATATACCATCAGATGAGGCAGTCAGGAGTTCATCCCGATGAATACACCTTAGCTACCCTCGTCAAAGCCGCCTCTTGTCTGACAGCATTGGAACAAGGGAAACAGATACATGCAGATGCAATAAAGTTGGAATATTCCTCAGACCCTTATGTTGCGACATCACTTGTTGACATGTATGCCAAGTGCGGTAATATAGAAGATGCATATCGTTTATTTAGAAGGATGGATGTCGGGAGCATTGTCCTGTGGAATGCCATGTTGGTGGGCTTTGCTCAACATGGAGACGCTGAAGAAGCACTAAATCTTTTCAGAGTCATGAAATCTAATAACATTTTGCCTGATAGAGTTACTTTCATTGGAGTTCTTTCTGCTTGCAGCCACTCTGGTTTAGTTTCTGAAGCTTACGAGCATTTTTCTTCAATGCAAAGAGATTACGGCATTGAACCTGAGATCGAGCACTACTCTTGTCTAGTCAATGCCCTTAGCCGCGCAGGGCGTGTTCAAGAGGCAGAAAAGCTGATAGCATCAATGCCATTTGAAGCTTCTGCTTCAATGTACAGAGCCCTGCTTGGTGCTTGCAGGGTTCAAGGGGACACAGAAACAGGAAAACGAGTAGCAGAACAGCTCTTGGCTATTGAGCCAACTGACTCATTGGCTTATGTTCTCATATCCAATATGAATGCTGCTGCCAACCAGTGGGATGCTGTGAAAGATGCTCGACGAATGATGAAACAGAAGAATGTAAAGAAAGAACCAGGTTTCAGTTGGATAGATGTGAAGAACAAGGTCCATTTGTTTCTGGTAGATGATAAATCCCATCCTGAAGCCGATTTGATATACGACAAGTTGGAGGACTTGATGAGATGGATCAGCGAAGAAGGGTATGTCCCTGATACAGAGTGTGCTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1771 PAM2 PPR PPR_2 Smr

Protein Analysis

939

Amino Acids

104.54

Weight (kDa)

6.05

Isoelectric Point (pI)

41.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 216 - 263 7.1e-07 PPR repeat family
PPR_2 PF13041 411 - 458 6.9e-07 PPR repeat family
PPR_2 PF13041 613 - 659 1.1e-08 PPR repeat family
PPR_2 PF13041 715 - 762 2.5e-10 PPR repeat family
PPR PF01535 717 - 747 1.5e-06 PPR repeat
E_motif PF20431 834 - 894 1.3e-14 E motif
Eplus_motif PF20430 899 - 924 3.9e-08 E+ motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 869
Acc16I TGCGCA 1 cut(s) 1809
Acc36I ACCTGC 1 cut(s) 273
Acc65I GGTACC 2 cut(s) 572, 1696
AccB1I GGYRCC 2 cut(s) 572, 1696
AccB7I CCANNNNNTGG 1 cut(s) 2604
AccII CGCG 2 cut(s) 201, 2397
AciI CCGC 7 cut(s) 160, 165, 424, 1275, 1977, 2094, 2395
AclWI GGATC 2 cut(s) 905, 2783
AcsI RAATTY 1 cut(s) 1710
AcuI CTGAAG 5 cut(s) 683, 695, 2217, 2325, 2751
AfaI GTAC 6 cut(s) 574, 821, 1571, 1698, 1781, 2465
AfiI CCNNNNNNNGG 2 cut(s) 334, 2604
AflIII ACRYGT 2 cut(s) 1167, 2079
AhlI ACTAGT 1 cut(s) 1676
AjnI CCWGG 5 cut(s) 333, 341, 576, 1683, 2662
AjuI GAANNNNNNNTTGG 8 cut(s) 36, 68, 228, 260, 530, 562, 2150, 2182
Alw21I GWGCWC 1 cut(s) 2367
Alw26I GTCTC 5 cut(s) 112, 324, 1635, 1788, 2184
AlwI GGATC 2 cut(s) 905, 2783
Ama87I CYCGRG 1 cut(s) 618
AoxI GGCC 3 cut(s) 379, 412, 1671
ApaI GGGCCC 1 cut(s) 383
ApoI RAATTY 1 cut(s) 1710
ArsI GACNNNNNNTTYG 2 cut(s) 269, 301
AseI ATTAAT 1 cut(s) 48
Asp718I GGTACC 2 cut(s) 572, 1696
AspLEI GCGC 5 cut(s) 201, 203, 1810, 1899, 2399
AspS9I GGNCC 5 cut(s) 379, 380, 749, 1231, 2695
AsuC2I CCSGG 1 cut(s) 1701
AsuHPI GGTGA 7 cut(s) 53, 256, 331, 439, 592, 986, 1051
AsuII TTCGAA 1 cut(s) 275
AvaI CYCGRG 1 cut(s) 618
AvaII GGWCC 3 cut(s) 749, 1231, 2695
AxyI CCTNAGG 1 cut(s) 223
BaeGI GKGCMC 1 cut(s) 383
BaeI ACNNNNGTAYC 2 cut(s) 564, 597
BanI GGYRCC 2 cut(s) 572, 1696
BanII GRGCYC 3 cut(s) 383, 1512, 2473
BbsI GAAGAC 1 cut(s) 689
Bbv12I GWGCWC 1 cut(s) 2367
BceAI ACGGC 1 cut(s) 2359
BciT130I CCWGG 5 cut(s) 335, 343, 578, 1685, 2664
BclI TGATCA 1 cut(s) 1336
BcnI CCSGG 1 cut(s) 1701
BcoDI GTCTC 5 cut(s) 112, 324, 1635, 1788, 2184
BcuI ACTAGT 1 cut(s) 1676
BfaI CTAG 6 cut(s) 356, 1235, 1677, 1772, 1877, 2378
BfmI CTRYAG 1 cut(s) 24
BfoI RGCGCY 1 cut(s) 1900
BfuAI ACCTGC 1 cut(s) 273
Bme1390I CCNGG 6 cut(s) 335, 343, 578, 1685, 1701, 2664
Bme18I GGWCC 3 cut(s) 749, 1231, 2695
BmeT110I CYCGRG 1 cut(s) 618
BmgT120I GGNCC 5 cut(s) 379, 380, 749, 1231, 2695
BmiI GGNNCC 5 cut(s) 380, 381, 574, 1509, 1698
BmrFI CCNGG 6 cut(s) 335, 343, 578, 1685, 1701, 2664
BmsI GCATC 8 cut(s) 627, 877, 1402, 2014, 2098, 2441, 2599, 2611
BpiI GAAGAC 1 cut(s) 689
BplI GAGNNNNNCTC 2 cut(s) 2354, 2386
Bpu10I CCTNAGC 3 cut(s) 1668, 1957, 2390
Bpu14I TTCGAA 1 cut(s) 275
BpuMI CCSGG 1 cut(s) 1701
BsaBI GATNNNNATC 2 cut(s) 891, 2112
BsaI GGTCTC 1 cut(s) 324
BsaJI CCNNGG 4 cut(s) 143, 190, 576, 1700
Bsc4I CCNNNNNNNGG 2 cut(s) 334, 2604
Bse1I ACTGG 3 cut(s) 450, 1435, 2602
Bse21I CCTNAGG 1 cut(s) 223
Bse3DI GCAATG 1 cut(s) 168
Bse8I GATNNNNATC 2 cut(s) 891, 2112
BseBI CCWGG 5 cut(s) 335, 343, 578, 1685, 2664
BseDI CCNNGG 4 cut(s) 143, 190, 576, 1700
BseJI GATNNNNATC 2 cut(s) 891, 2112
BseLI CCNNNNNNNGG 2 cut(s) 334, 2604
BseMI GCAATG 1 cut(s) 168
BseMII CTCAG 7 cut(s) 237, 613, 1411, 1881, 1914, 2061, 2346
BseNI ACTGG 3 cut(s) 450, 1435, 2602
BsePI GCGCGC 1 cut(s) 199
BseRI GAGGAG 3 cut(s) 91, 842, 1363
BseSI GKGCMC 1 cut(s) 383
BseYI CCCAGC 2 cut(s) 374, 853
BsgI GTGCAG 1 cut(s) 1818
Bsh1236I CGCG 2 cut(s) 201, 2397
BshFI GGCC 3 cut(s) 381, 414, 1673
BshNI GGYRCC 2 cut(s) 572, 1696
BsiHKAI GWGCWC 1 cut(s) 2367
BsiHKCI CYCGRG 1 cut(s) 618
BsiSI CCGG 2 cut(s) 421, 1700
BslFI GGGAC 3 cut(s) 420, 2513, 2780
BslI CCNNNNNNNGG 2 cut(s) 334, 2604
BsmAI GTCTC 5 cut(s) 112, 324, 1635, 1788, 2184
BsmBI CGTCTC 2 cut(s) 1635, 2184
BsmFI GGGAC 3 cut(s) 420, 2513, 2780
BsmI GAATGC 5 cut(s) 236, 352, 1558, 2164, 2593
BsnI GGCC 3 cut(s) 381, 414, 1673
Bso31I GGTCTC 1 cut(s) 324
BsoBI CYCGRG 1 cut(s) 618
Bsp119I TTCGAA 1 cut(s) 275
Bsp120I GGGCCC 1 cut(s) 379
Bsp1286I GDGCHC 4 cut(s) 383, 1512, 2367, 2473
Bsp1407I TGTACA 2 cut(s) 1779, 2463
Bsp143I GATC 6 cut(s) 238, 910, 1336, 1537, 2358, 2775
Bsp19I CCATGG 1 cut(s) 143
BspACI CCGC 7 cut(s) 160, 165, 424, 1275, 1977, 2094, 2395
BspANI GGCC 3 cut(s) 381, 414, 1673
BspCNI CTCAG 7 cut(s) 236, 612, 1410, 1880, 1913, 2060, 2347
BspFNI CGCG 2 cut(s) 201, 2397
BspHI TCATGA 2 cut(s) 1621, 2223
BspLI GGNNCC 5 cut(s) 380, 381, 574, 1509, 1698
BspMAI CTGCAG 1 cut(s) 28
BspMI ACCTGC 1 cut(s) 273
BspPI GGATC 2 cut(s) 905, 2783
BspT104I TTCGAA 1 cut(s) 275
BspT107I GGYRCC 2 cut(s) 572, 1696
BspTNI GGTCTC 1 cut(s) 324
BsrDI GCAATG 1 cut(s) 168
BsrGI TGTACA 2 cut(s) 1779, 2463
BsrI ACTGG 3 cut(s) 450, 1435, 2602
BssECI CCNNGG 4 cut(s) 143, 190, 576, 1700
BssHII GCGCGC 1 cut(s) 199
BssMI GATC 6 cut(s) 238, 910, 1336, 1537, 2358, 2775
BssT1I CCWWGG 1 cut(s) 143
Bst2UI CCWGG 5 cut(s) 335, 343, 578, 1685, 2664
Bst4CI ACNGT 3 cut(s) 1589, 1663, 1816
Bst6I CTCTTC 4 cut(s) 95, 142, 598, 1383
BstAPI GCANNNNNTGC 2 cut(s) 365, 1664
BstAUI TGTACA 2 cut(s) 1779, 2463
BstBI TTCGAA 1 cut(s) 275
BstC8I GCNNGC 7 cut(s) 201, 301, 1183, 1369, 2281, 2485, 2815
BstDSI CCRYGG 1 cut(s) 143
BstEII GGTNACC 1 cut(s) 337
BstFNI CGCG 2 cut(s) 201, 2397
BstH2I RGCGCY 1 cut(s) 1900
BstHHI GCGC 5 cut(s) 201, 203, 1810, 1899, 2399
BstKTI GATC 6 cut(s) 241, 913, 1339, 1540, 2361, 2778
BstMAI GTCTC 5 cut(s) 112, 324, 1635, 1788, 2184
BstMBI GATC 6 cut(s) 238, 910, 1336, 1537, 2358, 2775
BstMWI GCNNNNNNNGC 7 cut(s) 356, 365, 487, 496, 845, 1664, 2201
BstNI CCWGG 5 cut(s) 335, 343, 578, 1685, 2664
BstNSI RCATGY 3 cut(s) 1171, 2021, 2083
BstPI GGTNACC 1 cut(s) 337
BstSCI CCNGG 6 cut(s) 333, 341, 576, 1683, 1699, 2662
BstSFI CTRYAG 1 cut(s) 24
BstSLI GKGCMC 1 cut(s) 383
BstUI CGCG 2 cut(s) 201, 2397
BstV2I GAAGAC 1 cut(s) 689
BstXI CCANNNNNNTGG 1 cut(s) 2170
Bsu36I CCTNAGG 1 cut(s) 223
BsuRI GGCC 3 cut(s) 381, 414, 1673
BtgI CCRYGG 1 cut(s) 143
BtsIMutI CAGTG 2 cut(s) 1908, 2609
BveI ACCTGC 1 cut(s) 273
Cac8I GCNNGC 7 cut(s) 201, 301, 1183, 1369, 2281, 2485, 2815
CciI TCATGA 2 cut(s) 1621, 2223
CfoI GCGC 5 cut(s) 201, 203, 1810, 1899, 2399
Cfr13I GGNCC 5 cut(s) 379, 380, 749, 1231, 2695
CseI GACGC 1 cut(s) 2201
CsiI ACCWGGT 2 cut(s) 333, 2662
Csp6I GTAC 6 cut(s) 573, 820, 1570, 1697, 1780, 2464
CviQI GTAC 6 cut(s) 573, 820, 1570, 1697, 1780, 2464
DpnI GATC 6 cut(s) 240, 912, 1338, 1539, 2360, 2777
DpnII GATC 6 cut(s) 238, 910, 1336, 1537, 2358, 2775
Eam1104I CTCTTC 4 cut(s) 95, 142, 598, 1383
EarI CTCTTC 4 cut(s) 95, 142, 598, 1383
Eco130I CCWWGG 1 cut(s) 143
Eco24I GRGCYC 3 cut(s) 383, 1512, 2473
Eco31I GGTCTC 1 cut(s) 324
Eco32I GATATC 1 cut(s) 1257
Eco47I GGWCC 3 cut(s) 749, 1231, 2695
Eco57I CTGAAG 5 cut(s) 683, 695, 2217, 2325, 2751
Eco81I CCTNAGG 1 cut(s) 223
Eco88I CYCGRG 1 cut(s) 618
Eco91I GGTNACC 1 cut(s) 337
EcoO109I RGGNCCY 3 cut(s) 379, 380, 749
EcoO65I GGTNACC 1 cut(s) 337
EcoRII CCWGG 5 cut(s) 333, 341, 576, 1683, 2662
EcoRV GATATC 1 cut(s) 1257
EcoT14I CCWWGG 1 cut(s) 143
EcoT22I ATGCAT 2 cut(s) 1558, 2113
EcoT38I GRGCYC 3 cut(s) 383, 1512, 2473
ErhI CCWWGG 1 cut(s) 143
Esp3I CGTCTC 2 cut(s) 1635, 2184
FalI AAGNNNNNCTT 2 cut(s) 696, 728
FaqI GGGAC 3 cut(s) 420, 2513, 2780
FauNDI CATATG 3 cut(s) 295, 682, 841
FbaI TGATCA 1 cut(s) 1336
FriOI GRGCYC 3 cut(s) 383, 1512, 2473
FspAI RTGCGCAY 1 cut(s) 1809
FspBI CTAG 6 cut(s) 356, 1235, 1677, 1772, 1877, 2378
FspI TGCGCA 1 cut(s) 1809
GlaI GCGC 5 cut(s) 200, 202, 1809, 1898, 2398
GsaI CCCAGC 2 cut(s) 378, 857
HaeII RGCGCY 1 cut(s) 1900
HaeIII GGCC 3 cut(s) 381, 414, 1673
HapII CCGG 2 cut(s) 421, 1700
HgaI GACGC 1 cut(s) 2201
HhaI GCGC 5 cut(s) 201, 203, 1810, 1899, 2399
Hin6I GCGC 5 cut(s) 199, 201, 1808, 1897, 2397
HinP1I GCGC 5 cut(s) 199, 201, 1808, 1897, 2397
HincII GTYRAC 3 cut(s) 219, 1759, 2077
HindII GTYRAC 3 cut(s) 219, 1759, 2077
HindIII AAGCTT 2 cut(s) 2307, 2448
HinfI GANTC 9 cut(s) 408, 434, 787, 1102, 1133, 1289, 1618, 2220, 2555
HpaI GTTAAC 2 cut(s) 219, 1759
HpaII CCGG 2 cut(s) 421, 1700
HphI GGTGA 7 cut(s) 53, 256, 331, 439, 592, 986, 1051
Hpy99I CGWCG 1 cut(s) 2631
HpyAV CCTTC 7 cut(s) 670, 803, 1379, 1619, 1886, 2121, 2780
HpyCH4III ACNGT 3 cut(s) 1589, 1663, 1816
HpyF10VI GCNNNNNNNGC 7 cut(s) 356, 365, 487, 496, 845, 1664, 2201
HspAI GCGC 5 cut(s) 199, 201, 1808, 1897, 2397
KpnI GGTACC 2 cut(s) 576, 1700
Ksp22I TGATCA 1 cut(s) 1336
KspAI GTTAAC 2 cut(s) 219, 1759
Kzo9I GATC 6 cut(s) 238, 910, 1336, 1537, 2358, 2775
LmnI GCTCC 3 cut(s) 1376, 1507, 2142
LweI GCATC 8 cut(s) 627, 877, 1402, 2014, 2098, 2441, 2599, 2611
MabI ACCWGGT 2 cut(s) 333, 2662
MaeI CTAG 6 cut(s) 356, 1235, 1677, 1772, 1877, 2378
MaeIII GTNAC 5 cut(s) 337, 527, 1039, 1589, 2254
MalI GATC 6 cut(s) 240, 912, 1338, 1539, 2360, 2777
MboI GATC 6 cut(s) 238, 910, 1336, 1537, 2358, 2775
MfeI CAATTG 1 cut(s) 1347
MhlI GDGCHC 4 cut(s) 383, 1512, 2367, 2473
MlyI GAGTC 4 cut(s) 402, 443, 2229, 2549
MmeI TCCRAC 9 cut(s) 62, 135, 528, 920, 951, 1006, 2016, 2654, 2736
Mph1103I ATGCAT 2 cut(s) 1558, 2113
MseI TTAA 6 cut(s) 48, 218, 609, 1361, 1530, 1758
MslI CAYNNNNRTG 9 cut(s) 266, 738, 762, 1416, 1626, 1719, 1944, 2022, 2168
MspA1I CMGCKG 1 cut(s) 23
MspI CCGG 2 cut(s) 421, 1700
MspR9I CCNGG 6 cut(s) 335, 343, 578, 1685, 1701, 2664
MunI CAATTG 1 cut(s) 1347
Mva1269I GAATGC 5 cut(s) 236, 352, 1558, 2164, 2593
MvaI CCWGG 5 cut(s) 335, 343, 578, 1685, 2664
MvnI CGCG 2 cut(s) 201, 2397
MwoI GCNNNNNNNGC 7 cut(s) 356, 365, 487, 496, 845, 1664, 2201
NciI CCSGG 1 cut(s) 1701
NcoI CCATGG 1 cut(s) 143
NdeI CATATG 3 cut(s) 295, 682, 841
NdeII GATC 6 cut(s) 238, 910, 1336, 1537, 2358, 2775
NlaIV GGNNCC 5 cut(s) 380, 381, 574, 1509, 1698
NmeAIII GCCGAG 1 cut(s) 390
NmuCI GTSAC 3 cut(s) 337, 1039, 1589
NsbI TGCGCA 1 cut(s) 1809
NsiI ATGCAT 2 cut(s) 1558, 2113
NspI RCATGY 3 cut(s) 1171, 2021, 2083
NspV TTCGAA 1 cut(s) 275
PagI TCATGA 2 cut(s) 1621, 2223
PauI GCGCGC 1 cut(s) 199
PciI ACATGT 2 cut(s) 1167, 2079
PctI GAATGC 5 cut(s) 236, 352, 1558, 2164, 2593
PfeI GAWTC 5 cut(s) 787, 1102, 1133, 1289, 1618
PflFI GACNNNGTC 1 cut(s) 335
PflMI CCANNNNNTGG 1 cut(s) 2604
PleI GAGTC 4 cut(s) 402, 442, 2228, 2549
PpsI GAGTC 4 cut(s) 402, 442, 2228, 2549
PpuMI RGGWCCY 1 cut(s) 749
PscI ACATGT 2 cut(s) 1167, 2079
PshBI ATTAAT 1 cut(s) 48
PsiI TTATAA 1 cut(s) 869
Psp5II RGGWCCY 1 cut(s) 749
Psp6I CCWGG 5 cut(s) 333, 341, 576, 1683, 2662
PspEI GGTNACC 1 cut(s) 337
PspFI CCCAGC 2 cut(s) 374, 853
PspGI CCWGG 5 cut(s) 333, 341, 576, 1683, 2662
PspN4I GGNNCC 5 cut(s) 380, 381, 574, 1509, 1698
PspOMI GGGCCC 1 cut(s) 379
PspPI GGNCC 5 cut(s) 379, 380, 749, 1231, 2695
PspPPI RGGWCCY 1 cut(s) 749
PstI CTGCAG 1 cut(s) 28
PsyI GACNNNGTC 1 cut(s) 335
PteI GCGCGC 1 cut(s) 199
PvuII CAGCTG 1 cut(s) 23
RsaI GTAC 6 cut(s) 574, 821, 1571, 1698, 1781, 2465
RsaNI GTAC 6 cut(s) 573, 820, 1570, 1697, 1780, 2464
RseI CAYNNNNRTG 9 cut(s) 266, 738, 762, 1416, 1626, 1719, 1944, 2022, 2168
SaqAI TTAA 6 cut(s) 48, 218, 609, 1361, 1530, 1758
Sau3AI GATC 6 cut(s) 238, 910, 1336, 1537, 2358, 2775
Sau96I GGNCC 5 cut(s) 379, 380, 749, 1231, 2695
SchI GAGTC 4 cut(s) 402, 443, 2229, 2549
ScrFI CCNGG 6 cut(s) 335, 343, 578, 1685, 1701, 2664
SduI GDGCHC 4 cut(s) 383, 1512, 2367, 2473
SexAI ACCWGGT 2 cut(s) 333, 2662
SfaNI GCATC 8 cut(s) 627, 877, 1402, 2014, 2098, 2441, 2599, 2611
SfcI CTRYAG 1 cut(s) 24
SfuI TTCGAA 1 cut(s) 275
SinI GGWCC 3 cut(s) 749, 1231, 2695
SmiMI CAYNNNNRTG 9 cut(s) 266, 738, 762, 1416, 1626, 1719, 1944, 2022, 2168
SpeI ACTAGT 1 cut(s) 1676
SsiI CCGC 7 cut(s) 160, 165, 424, 1275, 1977, 2094, 2395
SspI AATATT 1 cut(s) 2042
SspMI CTAG 6 cut(s) 356, 1235, 1677, 1772, 1877, 2378
StyD4I CCNGG 6 cut(s) 333, 341, 576, 1683, 1699, 2662
StyI CCWWGG 1 cut(s) 143
TaaI ACNGT 3 cut(s) 1589, 1663, 1816
TaqI TCGA 7 cut(s) 275, 312, 1019, 1250, 1536, 2361, 2626
TaqII GACCGA 1 cut(s) 342
TatI WGTACW 2 cut(s) 1779, 2463
TauI GCSGC 3 cut(s) 427, 1979, 2397
TfiI GAWTC 5 cut(s) 787, 1102, 1133, 1289, 1618
Tru1I TTAA 6 cut(s) 48, 218, 609, 1361, 1530, 1758
Tru9I TTAA 6 cut(s) 48, 218, 609, 1361, 1530, 1758
TscAI CASTG 2 cut(s) 1908, 2609
TseFI GTSAC 3 cut(s) 337, 1039, 1589
Tsp45I GTSAC 3 cut(s) 337, 1039, 1589
TspGWI ACGGA 1 cut(s) 460
TspRI CASTG 2 cut(s) 1908, 2609
Tth111I GACNNNGTC 1 cut(s) 335
Van91I CCANNNNNTGG 1 cut(s) 2604
VpaK11BI GGWCC 3 cut(s) 749, 1231, 2695
VspI ATTAAT 1 cut(s) 48
XapI RAATTY 1 cut(s) 1710
XbaI TCTAGA 2 cut(s) 1771, 1876
XceI RCATGY 3 cut(s) 1171, 2021, 2083
XcmI CCANNNNNNNNNTGG 2 cut(s) 1681, 2670
XspI CTAG 6 cut(s) 356, 1235, 1677, 1772, 1877, 2378
Zsp2I ATGCAT 2 cut(s) 1558, 2113
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.