Rh6CG526300

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0007813
Physical Location & Seq
Forward (+)
68089221 .. 68092879
3659 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG526300.1

Sequence Viewer

Length: 3054 bp
ATGCAGTTGCACACCAATCTCCGACCCAAATCAATTTTCTTCTCCACCAAAAACCCCAAAACCCTTCTCCTTCCTCTCTTCAATTTCTCCTCTCTCTCCAACCCCATCTCTCACTCGCAACCCTCTTCTCCATGGTTCTCTATCCTCCGCTCCGCCATTGCCAATTCAGACTTGCCCATCGGCAAGCGCGCCCACGCCCTTATGTTAACCTCAGGGGAATGCCAAGACCATTTTCTAATCAACAATCTCATCACCATGTATTCGAAATGCAGGTCGCTCACGTATGCACGCCACCTGTTCGATAAAAGTCCTGACCGAGACCTGGTCACCTGGAATGCCATTCTAGCAGCCTATGCCCAAGCTGGGGGCTCTGTGGTTGAGAATGTTCAAGAGGGACTCGGCCTTTTCCGGCGGCTTCGTGAGTCTGTTGTGTTCACCAGTCGGCACACTTTGGCTTCCGTTTTGAAGCTGTGTTTGCTGTCGGGGCGTGTTTGGATTTCGGAGGCTGTTCAGGGGTACGCTGTGAAAGTTGGGTTGGAGTGGGATGTGTTTGTTTCGGGTACCCTGGTGAATGTGTATTGCAAACTCAGAAGAGTTAAGGAGGCTCGGGCTTTGTTTGATGGGATGCTGGAACGGGATGTTGTGTTGTGGAACACAATGCTGAAGGCATATGTGGAAATGGGTCTTCAGGAAGAAGCACTTGCTCTTTTCTCCAAGTTTCATCGGAGTGGATTGGGTCCTGATGATGTCAGTGTGCGTTGTGTTCTTAGTGGGATTCACAAAGTTGATTATGATGAAGGCAAGAGGCACATGGAGGAGGTTCGAGCATATGCAGCAAAGCTGGGGGAGTTTATAACTAATGTGGATGATGAAGCCCATCCACCAAGCTCTTGTGTTCCCACTACTAATTCCGACATATTTTTGTGGAACAAGACAGTGTCCAAGTATATCCGAGCTGGTGAAAATTGGGCTGCGATAGAGTGCTTTAGAAATATGATTAGATCAAAAGTTGGATATGACAGTGTGACATTAGTTATTATTCTATCTGCGATTGTGGGCACAAATAATTTGGAGGTGGGGAAACAGATTCACGGTGCTGCTTTGAAATCAGATTTTGGTTCTGTTCTTTTAGTAGCAAATAGCCTTATCAACATGTATTCAAAGGCTCGCTGTGTTCACTTTGCGAGAGAAGTATTCAATCACATGAAAGAAGTGGACCTAGTTTCATGGAACTCGATGATATCATGTTATGCTCAAAGCGGTTCAGGAGAGGAATCAATAAATCTATTTATAGGTTTATTACGGGATGGTCTAAGACCTGATCAATTTACAATTGCAAGTATTTTAAGGGCTTGCTCCTCTCTTCAAGAAGGATTGCATCTCAGTGAACAGGTTCATGTTCATGCAATAAAGAACGGTATTGCTGCTGACAGATTTGTTTCAACAGCTCTTATTGATGTTTATTCTAGAAGTGGAAAGATGGACGAGGCGGAGGCCCTTTTAGATAACAAAGTTAAATTCGATCTGGCATCTTGGAATGCATTGATGTTTGGGTACATAAAGAGTAATGACAGTCACAAAGCACTACAACTTATGAGGATGATTCATGAAGGTGGAAAGAGAGTAGATGAGATTACTTTGGCAACTGTTGCTAAGGCCACTAGTTGCCTGGTAGCGTTGGTACCGGGGAAGCAAATTCATGCTCATGTCATAAAAACAGGGTATTCAAAGGACTTATATGTTAACAGCAGTATTCTAGATATGTACATAAAATGTGGAGACATGAAAAGTGCACATACAGTTTTCAATGACATTCCTGCACCTGATGATGTTGCGTGGACAACTCTAATCTCAGGATGTTTAGAAAATGGAGATGAAGGGCGCTCTGTGTCTATATACCATCAGATGAGGCAGTCAGGAGTTCAGCCTGATGAATACACCTTAGCTACCCTTGTCAAAGCCGCCTCTTGTCTGACAGCATTGGAACAAGGGAAACAGATACATGCAGATGCAATAAAGTTGGAATATTCCTCAGACCCTTATGTTGCGACATCACTTGTTGACATGTATGCCAAGTGCGGTAACATAGAAGGTGCATATCGTTTATTCAGAAGGATGGATGTCGGGAGCATTGTCCTGTGGAATGCCATGTTGGTGGGTTTAGCTCAACATGGAGACGCTGAAGAAGCACTAAATCTTTTCAGAGTCATGAAATCTAATAACATTTTGCCTGATAGAGTTACTTTCATTGGAGTTCTTTCTGCTTGCAGCCACTCTGGTTTAGTTTCTGAAGCTTACGAGCATTTTTCTTCAATGCAAAGAGATTACGGCATTGAACCTGAGATCGAGCACTACTCTTGTCTAGTCGATGCCCTTGGCCGCGCAGGGCGTGTTCAAGAGGCAGAAAAGCTGATAGCATCAATGCCATTTGAAGCTTCTGCTTCAATGTACAGAGCCCTGCTTGGTGCTTGCAGGGTTCAAGGGGACACAGAAACAGGAAAACGAGTAGCAGAACAGCTCTTGGCTATTGAGCCAACTGACTCATCTGCGTATGTTCTCATATCCAATATGAATGCTGCTGCCAACCAGTGGGATGCTGTGAACGATGCTCGTCAAATGATGAAACAGAAGAATGTAAAGAAAGAACCAGGTTTCAGTTGGATAGATGTGAAGAACATGGTTCATTTGTTTGTAGTAGATGACAAATCCCATCCTGAAGCCGATTTGATATACGACAAGTTGGAGGACTTGATGAAACGGATCGGTGAAGAAGGGTATGTCCCTGACACAGAGTTTGTGCTTGCTGATGTTGAAGAAGAGGAAAAAGAGCGAGCCCTATATTACCATAGTGAGAGGCTAGCAATAGCTTATGGACTGATAAGCACTCCCTCATCTGCCATTATTCGGGTCATTAAGAACCTTAGAGTTTGTGGAGACTGCCATAATGCAATCAAATACATATCAAAGGTTTCTCATAGAGAAATTGTGCTGAGAGATGCAAATCGCTTCCATCGTTTCAGAAATGGGAGCTGCTCCTGTGGTGATTACTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1771 PAM2 PPR PPR_2 Smr

Protein Analysis

1017

Amino Acids

113.29

Weight (kDa)

6.39

Isoelectric Point (pI)

41.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 212 - 259 3.5e-07 PPR repeat family
PPR_2 PF13041 406 - 453 8.4e-07 PPR repeat family
PPR_2 PF13041 608 - 654 5.7e-09 PPR repeat family
PPR_2 PF13041 710 - 757 3.3e-10 PPR repeat family
PPR PF01535 712 - 742 2.6e-06 PPR repeat
E_motif PF20431 829 - 889 5.5e-16 E motif
Eplus_motif PF20430 894 - 921 1.2e-09 E+ motif
DYW_deaminase PF14432 925 - 1017 9.2e-44 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 854
Acc36I ACCTGC 1 cut(s) 261
Acc65I GGTACC 2 cut(s) 560, 1681
AccB1I GGYRCC 2 cut(s) 560, 1681
AccB7I CCANNNNNTGG 1 cut(s) 2589
AccBSI CCGCTC 1 cut(s) 150
AccII CGCG 2 cut(s) 189, 2382
AciI CCGC 8 cut(s) 148, 153, 412, 1260, 1490, 1962, 2079, 2380
AclWI GGATC 1 cut(s) 2768
AcoI YGGCCR 1 cut(s) 2377
AcsI RAATTY 2 cut(s) 1517, 1695
AcuI CTGAAG 5 cut(s) 671, 683, 2202, 2310, 2736
AfaI GTAC 6 cut(s) 518, 562, 1556, 1683, 1766, 2450
AfiI CCNNNNNNNGG 5 cut(s) 322, 363, 364, 2589, 2904
AflIII ACRYGT 2 cut(s) 1152, 2064
AhlI ACTAGT 1 cut(s) 1661
AjnI CCWGG 5 cut(s) 321, 329, 564, 1668, 2647
AjuI GAANNNNNNNTTGG 8 cut(s) 20, 52, 216, 248, 518, 550, 2135, 2167
Alw21I GWGCWC 2 cut(s) 1795, 2352
Alw26I GTCTC 4 cut(s) 312, 1773, 2169, 2928
Alw44I GTGCAC 1 cut(s) 1791
AlwI GGATC 1 cut(s) 2768
Ama87I CYCGRG 1 cut(s) 606
AoxI GGCC 4 cut(s) 400, 1494, 1656, 2377
ApaLI GTGCAC 1 cut(s) 1791
ApeKI GCWGC 9 cut(s) 347, 833, 971, 1097, 1424, 2268, 2576, 2579, 3030
ApoI RAATTY 2 cut(s) 1517, 1695
ArsI GACNNNNNNTTYG 4 cut(s) 257, 289, 2777, 2809
Asp700I GAANNNNTTC 1 cut(s) 1392
Asp718I GGTACC 2 cut(s) 560, 1681
AspLEI GCGC 4 cut(s) 189, 191, 1884, 2384
AspS9I GGNCC 3 cut(s) 737, 1216, 1495
AsuC2I CCSGG 1 cut(s) 1686
AsuHPI GGTGA 7 cut(s) 244, 319, 427, 580, 971, 2777, 3053
AsuII TTCGAA 1 cut(s) 263
AsuNHI GCTAGC 1 cut(s) 2857
AvaI CYCGRG 1 cut(s) 606
AvaII GGWCC 2 cut(s) 737, 1216
AxyI CCTNAGG 1 cut(s) 211
BaeGI GKGCMC 2 cut(s) 1061, 1795
BaeI ACNNNNGTAYC 2 cut(s) 552, 585
BanI GGYRCC 2 cut(s) 560, 1681
BanII GRGCYC 3 cut(s) 371, 2458, 2836
BbsI GAAGAC 1 cut(s) 677
Bbv12I GWGCWC 2 cut(s) 1795, 2352
BbvI GCAGC 9 cut(s) 359, 845, 958, 1084, 1411, 2280, 2563, 2566, 3017
BceAI ACGGC 1 cut(s) 2344
BciT130I CCWGG 5 cut(s) 323, 331, 566, 1670, 2649
BclI TGATCA 1 cut(s) 1321
BcnI CCSGG 1 cut(s) 1686
BcoDI GTCTC 4 cut(s) 312, 1773, 2169, 2928
BcuI ACTAGT 1 cut(s) 1661
BfaI CTAG 7 cut(s) 344, 1220, 1467, 1662, 1757, 2363, 2858
BfoI RGCGCY 1 cut(s) 1885
BfuAI ACCTGC 1 cut(s) 261
Bme1390I CCNGG 6 cut(s) 323, 331, 566, 1670, 1686, 2649
Bme18I GGWCC 2 cut(s) 737, 1216
BmeT110I CYCGRG 1 cut(s) 606
BmgT120I GGNCC 3 cut(s) 737, 1216, 1495
BmiI GGNNCC 3 cut(s) 562, 738, 1683
BmrFI CCNGG 6 cut(s) 323, 331, 566, 1670, 1686, 2649
BmsI GCATC 9 cut(s) 615, 1387, 1538, 1999, 2359, 2426, 2584, 2596, 2986
BmtI GCTAGC 1 cut(s) 2861
BpiI GAAGAC 1 cut(s) 677
BplI GAGNNNNNCTC 2 cut(s) 2339, 2371
Bpu10I CCTNAGC 2 cut(s) 1653, 1942
Bpu14I TTCGAA 1 cut(s) 263
BpuMI CCSGG 1 cut(s) 1686
BsaAI YACGTR 1 cut(s) 282
BsaBI GATNNNNATC 1 cut(s) 3000
BsaI GGTCTC 1 cut(s) 312
BsaJI CCNNGG 4 cut(s) 131, 564, 1685, 2374
Bsc4I CCNNNNNNNGG 5 cut(s) 322, 363, 364, 2589, 2904
Bse1I ACTGG 3 cut(s) 438, 2587, 3053
Bse21I CCTNAGG 1 cut(s) 211
Bse3DI GCAATG 1 cut(s) 156
Bse8I GATNNNNATC 1 cut(s) 3000
BseBI CCWGG 5 cut(s) 323, 331, 566, 1670, 2649
BseDI CCNNGG 4 cut(s) 131, 564, 1685, 2374
BseJI GATNNNNATC 1 cut(s) 3000
BseLI CCNNNNNNNGG 5 cut(s) 322, 363, 364, 2589, 2904
BseMI GCAATG 1 cut(s) 156
BseMII CTCAG 7 cut(s) 225, 601, 1396, 1866, 2046, 2331, 2981
BseNI ACTGG 3 cut(s) 438, 2587, 3053
BsePI GCGCGC 1 cut(s) 187
BseRI GAGGAG 3 cut(s) 79, 830, 1348
BseSI GKGCMC 2 cut(s) 1061, 1795
BseXI GCAGC 9 cut(s) 359, 845, 958, 1084, 1411, 2280, 2563, 2566, 3017
BseYI CCCAGC 2 cut(s) 362, 841
BsgI GTGCAG 1 cut(s) 1803
Bsh1236I CGCG 2 cut(s) 189, 2382
BshFI GGCC 4 cut(s) 402, 1496, 1658, 2379
BshNI GGYRCC 2 cut(s) 560, 1681
BsiHKAI GWGCWC 2 cut(s) 1795, 2352
BsiHKCI CYCGRG 1 cut(s) 606
BsiSI CCGG 2 cut(s) 409, 1685
BslFI GGGAC 3 cut(s) 408, 2498, 2765
BslI CCNNNNNNNGG 5 cut(s) 322, 363, 364, 2589, 2904
BsmAI GTCTC 4 cut(s) 312, 1773, 2169, 2928
BsmBI CGTCTC 1 cut(s) 2169
BsmFI GGGAC 3 cut(s) 408, 2498, 2765
BsmI GAATGC 5 cut(s) 224, 340, 1543, 2149, 2578
BsnI GGCC 4 cut(s) 402, 1496, 1658, 2379
Bso31I GGTCTC 1 cut(s) 312
BsoBI CYCGRG 1 cut(s) 606
Bsp119I TTCGAA 1 cut(s) 263
Bsp1286I GDGCHC 6 cut(s) 371, 1061, 1795, 2352, 2458, 2836
Bsp1407I TGTACA 2 cut(s) 1764, 2448
Bsp143I GATC 5 cut(s) 1001, 1321, 1522, 2343, 2760
Bsp19I CCATGG 1 cut(s) 131
BspACI CCGC 8 cut(s) 148, 153, 412, 1260, 1490, 1962, 2079, 2380
BspANI GGCC 4 cut(s) 402, 1496, 1658, 2379
BspCNI CTCAG 7 cut(s) 224, 600, 1395, 1865, 2045, 2332, 2982
BspFNI CGCG 2 cut(s) 189, 2382
BspHI TCATGA 2 cut(s) 1606, 2208
BspLI GGNNCC 3 cut(s) 562, 738, 1683
BspMI ACCTGC 1 cut(s) 261
BspOI GCTAGC 1 cut(s) 2861
BspPI GGATC 1 cut(s) 2768
BspT104I TTCGAA 1 cut(s) 263
BspT107I GGYRCC 2 cut(s) 560, 1681
BspTNI GGTCTC 1 cut(s) 312
BsrBI CCGCTC 1 cut(s) 150
BsrDI GCAATG 1 cut(s) 156
BsrGI TGTACA 2 cut(s) 1764, 2448
BsrI ACTGG 3 cut(s) 438, 2587, 3053
BssECI CCNNGG 4 cut(s) 131, 564, 1685, 2374
BssHII GCGCGC 1 cut(s) 187
BssMI GATC 5 cut(s) 1001, 1321, 1522, 2343, 2760
BssT1I CCWWGG 2 cut(s) 131, 2374
Bst2UI CCWGG 5 cut(s) 323, 331, 566, 1670, 2649
Bst4CI ACNGT 7 cut(s) 937, 1022, 1094, 1418, 1574, 1648, 1801
Bst6I CTCTTC 5 cut(s) 83, 130, 586, 1368, 2811
BstAPI GCANNNNNTGC 2 cut(s) 353, 1649
BstAUI TGTACA 2 cut(s) 1764, 2448
BstBAI YACGTR 1 cut(s) 282
BstBI TTCGAA 1 cut(s) 263
BstDSI CCRYGG 1 cut(s) 131
BstEII GGTNACC 1 cut(s) 325
BstFNI CGCG 2 cut(s) 189, 2382
BstH2I RGCGCY 1 cut(s) 1885
BstHHI GCGC 4 cut(s) 189, 191, 1884, 2384
BstKTI GATC 5 cut(s) 1004, 1324, 1525, 2346, 2763
BstMAI GTCTC 4 cut(s) 312, 1773, 2169, 2928
BstMBI GATC 5 cut(s) 1001, 1321, 1522, 2343, 2760
BstMWI GCNNNNNNNGC 7 cut(s) 344, 353, 475, 484, 833, 1649, 2186
BstNI CCWGG 5 cut(s) 323, 331, 566, 1670, 2649
BstNSI RCATGY 3 cut(s) 1156, 2006, 2068
BstPI GGTNACC 1 cut(s) 325
BstSCI CCNGG 6 cut(s) 321, 329, 564, 1668, 1684, 2647
BstSLI GKGCMC 2 cut(s) 1061, 1795
BstUI CGCG 2 cut(s) 189, 2382
BstV1I GCAGC 9 cut(s) 359, 845, 958, 1084, 1411, 2280, 2563, 2566, 3017
BstV2I GAAGAC 1 cut(s) 677
BstXI CCANNNNNNTGG 1 cut(s) 2155
Bsu36I CCTNAGG 1 cut(s) 211
BsuRI GGCC 4 cut(s) 402, 1496, 1658, 2379
BtgI CCRYGG 1 cut(s) 131
BtsIMutI CAGTG 5 cut(s) 757, 942, 1027, 1390, 2594
BveI ACCTGC 1 cut(s) 261
CciI TCATGA 2 cut(s) 1606, 2208
CfoI GCGC 4 cut(s) 189, 191, 1884, 2384
Cfr13I GGNCC 3 cut(s) 737, 1216, 1495
CseI GACGC 1 cut(s) 2186
CsiI ACCWGGT 2 cut(s) 321, 2647
Csp6I GTAC 6 cut(s) 517, 561, 1555, 1682, 1765, 2449
CviQI GTAC 6 cut(s) 517, 561, 1555, 1682, 1765, 2449
DpnI GATC 5 cut(s) 1003, 1323, 1524, 2345, 2762
DpnII GATC 5 cut(s) 1001, 1321, 1522, 2343, 2760
EaeI YGGCCR 1 cut(s) 2377
Eam1104I CTCTTC 5 cut(s) 83, 130, 586, 1368, 2811
EarI CTCTTC 5 cut(s) 83, 130, 586, 1368, 2811
EciI GGCGGA 2 cut(s) 142, 1505
Eco130I CCWWGG 2 cut(s) 131, 2374
Eco24I GRGCYC 3 cut(s) 371, 2458, 2836
Eco31I GGTCTC 1 cut(s) 312
Eco32I GATATC 1 cut(s) 1242
Eco47I GGWCC 2 cut(s) 737, 1216
Eco57I CTGAAG 5 cut(s) 671, 683, 2202, 2310, 2736
Eco81I CCTNAGG 1 cut(s) 211
Eco88I CYCGRG 1 cut(s) 606
Eco91I GGTNACC 1 cut(s) 325
EcoO109I RGGNCCY 2 cut(s) 737, 1495
EcoO65I GGTNACC 1 cut(s) 325
EcoRII CCWGG 5 cut(s) 321, 329, 564, 1668, 2647
EcoRV GATATC 1 cut(s) 1242
EcoT14I CCWWGG 2 cut(s) 131, 2374
EcoT22I ATGCAT 1 cut(s) 1543
EcoT38I GRGCYC 3 cut(s) 371, 2458, 2836
ErhI CCWWGG 2 cut(s) 131, 2374
Esp3I CGTCTC 1 cut(s) 2169
FalI AAGNNNNNCTT 2 cut(s) 684, 716
FaqI GGGAC 3 cut(s) 408, 2498, 2765
FauNDI CATATG 2 cut(s) 670, 829
FbaI TGATCA 1 cut(s) 1321
FriOI GRGCYC 3 cut(s) 371, 2458, 2836
FspBI CTAG 7 cut(s) 344, 1220, 1467, 1662, 1757, 2363, 2858
GlaI GCGC 4 cut(s) 188, 190, 1883, 2383
GsaI CCCAGC 2 cut(s) 366, 845
HaeII RGCGCY 1 cut(s) 1885
HaeIII GGCC 4 cut(s) 402, 1496, 1658, 2379
HapII CCGG 2 cut(s) 409, 1685
HgaI GACGC 1 cut(s) 2186
HhaI GCGC 4 cut(s) 189, 191, 1884, 2384
Hin6I GCGC 4 cut(s) 187, 189, 1882, 2382
HinP1I GCGC 4 cut(s) 187, 189, 1882, 2382
HincII GTYRAC 3 cut(s) 207, 1744, 2062
HindII GTYRAC 3 cut(s) 207, 1744, 2062
HindIII AAGCTT 2 cut(s) 2292, 2433
HinfI GANTC 8 cut(s) 396, 422, 775, 1087, 1274, 1603, 2205, 2540
HpaI GTTAAC 2 cut(s) 207, 1744
HpaII CCGG 2 cut(s) 409, 1685
HphI GGTGA 7 cut(s) 244, 319, 427, 580, 971, 2777, 3053
HpyCH4III ACNGT 7 cut(s) 937, 1022, 1094, 1418, 1574, 1648, 1801
HpyCH4IV ACGT 1 cut(s) 281
HpyF10VI GCNNNNNNNGC 7 cut(s) 344, 353, 475, 484, 833, 1649, 2186
HpySE526I ACGT 1 cut(s) 281
HspAI GCGC 4 cut(s) 187, 189, 1882, 2382
KpnI GGTACC 2 cut(s) 564, 1685
Ksp22I TGATCA 1 cut(s) 1321
KspAI GTTAAC 2 cut(s) 207, 1744
Kzo9I GATC 5 cut(s) 1001, 1321, 1522, 2343, 2760
LmnI GCTCC 5 cut(s) 155, 1361, 2127, 3027, 3038
Lsp1109I GCAGC 9 cut(s) 359, 845, 958, 1084, 1411, 2280, 2563, 2566, 3017
LweI GCATC 9 cut(s) 615, 1387, 1538, 1999, 2359, 2426, 2584, 2596, 2986
MabI ACCWGGT 2 cut(s) 321, 2647
MaeI CTAG 7 cut(s) 344, 1220, 1467, 1662, 1757, 2363, 2858
MaeII ACGT 1 cut(s) 281
MaeIII GTNAC 5 cut(s) 325, 1024, 1574, 2081, 2239
MalI GATC 5 cut(s) 1003, 1323, 1524, 2345, 2762
MbiI CCGCTC 1 cut(s) 150
MboI GATC 5 cut(s) 1001, 1321, 1522, 2343, 2760
MfeI CAATTG 1 cut(s) 1332
MhlI GDGCHC 6 cut(s) 371, 1061, 1795, 2352, 2458, 2836
MlyI GAGTC 4 cut(s) 390, 431, 2214, 2534
MmeI TCCRAC 8 cut(s) 46, 123, 516, 936, 991, 2001, 2639, 2721
Mph1103I ATGCAT 1 cut(s) 1543
MroXI GAANNNNTTC 1 cut(s) 1392
MseI TTAA 6 cut(s) 206, 597, 1346, 1515, 1743, 2913
MslI CAYNNNNRTG 8 cut(s) 254, 726, 1383, 1401, 1611, 1704, 2007, 2153
MspI CCGG 2 cut(s) 409, 1685
MspR9I CCNGG 6 cut(s) 323, 331, 566, 1670, 1686, 2649
MunI CAATTG 1 cut(s) 1332
Mva1269I GAATGC 5 cut(s) 224, 340, 1543, 2149, 2578
MvaI CCWGG 5 cut(s) 323, 331, 566, 1670, 2649
MvnI CGCG 2 cut(s) 189, 2382
MwoI GCNNNNNNNGC 7 cut(s) 344, 353, 475, 484, 833, 1649, 2186
NciI CCSGG 1 cut(s) 1686
NcoI CCATGG 1 cut(s) 131
NdeI CATATG 2 cut(s) 670, 829
NdeII GATC 5 cut(s) 1001, 1321, 1522, 2343, 2760
NheI GCTAGC 1 cut(s) 2857
NlaIV GGNNCC 3 cut(s) 562, 738, 1683
NmeAIII GCCGAG 1 cut(s) 378
NmuCI GTSAC 3 cut(s) 325, 1024, 1574
NsiI ATGCAT 1 cut(s) 1543
NspI RCATGY 3 cut(s) 1156, 2006, 2068
NspV TTCGAA 1 cut(s) 263
PagI TCATGA 2 cut(s) 1606, 2208
PauI GCGCGC 1 cut(s) 187
PciI ACATGT 2 cut(s) 1152, 2064
PcsI WCGNNNNNNNCGW 1 cut(s) 3010
PctI GAATGC 5 cut(s) 224, 340, 1543, 2149, 2578
PdmI GAANNNNTTC 1 cut(s) 1392
PfeI GAWTC 4 cut(s) 775, 1087, 1274, 1603
PflFI GACNNNGTC 2 cut(s) 323, 937
PflMI CCANNNNNTGG 1 cut(s) 2589
PleI GAGTC 4 cut(s) 390, 430, 2213, 2534
PpsI GAGTC 4 cut(s) 390, 430, 2213, 2534
Ppu21I YACGTR 1 cut(s) 282
PpuMI RGGWCCY 1 cut(s) 737
PscI ACATGT 2 cut(s) 1152, 2064
PsiI TTATAA 1 cut(s) 854
Psp5II RGGWCCY 1 cut(s) 737
Psp6I CCWGG 5 cut(s) 321, 329, 564, 1668, 2647
PspEI GGTNACC 1 cut(s) 325
PspFI CCCAGC 2 cut(s) 362, 841
PspGI CCWGG 5 cut(s) 321, 329, 564, 1668, 2647
PspN4I GGNNCC 3 cut(s) 562, 738, 1683
PspPI GGNCC 3 cut(s) 737, 1216, 1495
PspPPI RGGWCCY 1 cut(s) 737
PsyI GACNNNGTC 2 cut(s) 323, 937
PteI GCGCGC 1 cut(s) 187
RsaI GTAC 6 cut(s) 518, 562, 1556, 1683, 1766, 2450
RsaNI GTAC 6 cut(s) 517, 561, 1555, 1682, 1765, 2449
RseI CAYNNNNRTG 8 cut(s) 254, 726, 1383, 1401, 1611, 1704, 2007, 2153
SaqAI TTAA 6 cut(s) 206, 597, 1346, 1515, 1743, 2913
Sau3AI GATC 5 cut(s) 1001, 1321, 1522, 2343, 2760
Sau96I GGNCC 3 cut(s) 737, 1216, 1495
SchI GAGTC 4 cut(s) 390, 431, 2214, 2534
ScrFI CCNGG 6 cut(s) 323, 331, 566, 1670, 1686, 2649
SduI GDGCHC 6 cut(s) 371, 1061, 1795, 2352, 2458, 2836
SexAI ACCWGGT 2 cut(s) 321, 2647
SfaNI GCATC 9 cut(s) 615, 1387, 1538, 1999, 2359, 2426, 2584, 2596, 2986
SfuI TTCGAA 1 cut(s) 263
SinI GGWCC 2 cut(s) 737, 1216
SmiMI CAYNNNNRTG 8 cut(s) 254, 726, 1383, 1401, 1611, 1704, 2007, 2153
SpeI ACTAGT 1 cut(s) 1661
SsiI CCGC 8 cut(s) 148, 153, 412, 1260, 1490, 1962, 2079, 2380
SspI AATATT 1 cut(s) 2027
SspMI CTAG 7 cut(s) 344, 1220, 1467, 1662, 1757, 2363, 2858
StyD4I CCNGG 6 cut(s) 321, 329, 564, 1668, 1684, 2647
StyI CCWWGG 2 cut(s) 131, 2374
TaaI ACNGT 7 cut(s) 937, 1022, 1094, 1418, 1574, 1648, 1801
TaiI ACGT 1 cut(s) 284
TaqI TCGA 7 cut(s) 263, 300, 823, 1235, 1521, 2346, 2367
TaqII GACCGA 1 cut(s) 330
TatI WGTACW 2 cut(s) 1764, 2448
TauI GCSGC 3 cut(s) 415, 1964, 2382
TfiI GAWTC 4 cut(s) 775, 1087, 1274, 1603
Tru1I TTAA 6 cut(s) 206, 597, 1346, 1515, 1743, 2913
Tru9I TTAA 6 cut(s) 206, 597, 1346, 1515, 1743, 2913
TscAI CASTG 5 cut(s) 757, 942, 1027, 1390, 2594
TseFI GTSAC 3 cut(s) 325, 1024, 1574
TseI GCWGC 9 cut(s) 347, 833, 971, 1097, 1424, 2268, 2576, 2579, 3030
Tsp45I GTSAC 3 cut(s) 325, 1024, 1574
TspGWI ACGGA 2 cut(s) 448, 2773
TspRI CASTG 5 cut(s) 757, 942, 1027, 1390, 2594
Tth111I GACNNNGTC 2 cut(s) 323, 937
Van91I CCANNNNNTGG 1 cut(s) 2589
VneI GTGCAC 1 cut(s) 1791
VpaK11BI GGWCC 2 cut(s) 737, 1216
XapI RAATTY 2 cut(s) 1517, 1695
XbaI TCTAGA 2 cut(s) 1466, 1756
XceI RCATGY 3 cut(s) 1156, 2006, 2068
XcmI CCANNNNNNNNNTGG 2 cut(s) 1666, 2655
XmnI GAANNNNTTC 1 cut(s) 1392
XspI CTAG 7 cut(s) 344, 1220, 1467, 1662, 1757, 2363, 2858
Zsp2I ATGCAT 1 cut(s) 1543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.