MD15G1123100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0005492
Physical Location & Seq
Reverse (-)
8890361 .. 8891657
1297 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1123100.v1.1.491

Sequence Viewer

Length: 516 bp
ATGTGTACCACACCGTTGCATGTCACATCTTCCCAGCTGATTGCAAGTGAAATCTTTCCTGCTGCTGCAGTTAAGGCCCTCCTCTACCCCGGAGCTGTTGCAAATGGACTTGCAAAAAACATGACTGTCCCAAGTTGGAATAACCTATTGGAAGTCTATAACTTGACTAGTGCAAAGGAAGCCTCTCCGGTTACTGATCTCCAGCGCTTAGAGGTTCTAGCTGGAAGTTACTTCGCTGTGGCTGGAGCTCTTGTGGGAATTCTAAAACGAGGGAGGATGAGCATGTTCGGGATGCTTCTAGTTCTCTGGGGCCTCATCAAAGAAGGGATTCTCGGAAAGCCCGTGAACACAGACCCTACAAAAGCTGTGTTTGTCTACCCAACCATGCTCATTGCTGTAATCGCTGCTTTCTCGTCTGTAAAGTATAATATGAAGAAGGCTGCTGCAAGAAGTGCACCTGCAGCAAGGCCCATTGCAAAGCCTCTTCAAAGGTCTTCAAAATCTAAGCTGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

172

Amino Acids

18.2

Weight (kDa)

10.14

Isoelectric Point (pI)

39.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 466
Acc36I ACCTGC 1 cut(s) 466
AccI GTMKAC 1 cut(s) 375
AcsI RAATTY 1 cut(s) 258
AfaI GTAC 1 cut(s) 7
AfeI AGCGCT 1 cut(s) 206
AgsI TTSAA 2 cut(s) 488, 498
AhlI ACTAGT 1 cut(s) 167
AjuI GAANNNNNNNTTGG 2 cut(s) 131, 163
AluBI AGCT 6 cut(s) 37, 95, 221, 248, 365, 508
AluI AGCT 6 cut(s) 37, 95, 221, 248, 365, 508
Alw21I GWGCWC 2 cut(s) 250, 457
Alw44I GTGCAC 1 cut(s) 453
Aor51HI AGCGCT 1 cut(s) 206
AoxI GGCC 3 cut(s) 75, 310, 467
ApaLI GTGCAC 1 cut(s) 453
ApeKI GCWGC 6 cut(s) 62, 65, 404, 440, 443, 461
ApoI RAATTY 1 cut(s) 258
Asp700I GAANNNNTTC 2 cut(s) 54, 327
AspLEI GCGC 1 cut(s) 207
AspS9I GGNCC 3 cut(s) 76, 310, 468
AsuC2I CCSGG 1 cut(s) 90
BaeGI GKGCMC 1 cut(s) 457
BanII GRGCYC 1 cut(s) 250
BbsI GAAGAC 1 cut(s) 486
Bbv12I GWGCWC 2 cut(s) 250, 457
BbvI GCAGC 6 cut(s) 49, 52, 391, 427, 430, 473
BcnI CCSGG 1 cut(s) 90
BcuI ACTAGT 1 cut(s) 167
BfaI CTAG 3 cut(s) 168, 218, 299
BfmI CTRYAG 2 cut(s) 66, 459
BfoI RGCGCY 1 cut(s) 208
BfuAI ACCTGC 1 cut(s) 466
BisI GCNGC 6 cut(s) 63, 66, 405, 441, 444, 462
BlsI GCNGC 6 cut(s) 64, 67, 406, 442, 445, 463
Bme1390I CCNGG 1 cut(s) 90
BmgT120I GGNCC 3 cut(s) 76, 310, 468
BmiI GGNNCC 1 cut(s) 311
BmrFI CCNGG 1 cut(s) 90
BmsI GCATC 1 cut(s) 282
BpiI GAAGAC 1 cut(s) 486
BpmI CTGGAG 2 cut(s) 185, 264
BpuMI CCSGG 1 cut(s) 90
BsaJI CCNNGG 1 cut(s) 88
BsaWI WCCGGW 1 cut(s) 187
BsaXI ACNNNNNCTCC 2 cut(s) 237, 267
Bse3DI GCAATG 2 cut(s) 390, 471
BseDI CCNNGG 1 cut(s) 88
BseGI GGATG 2 cut(s) 282, 297
BseMI GCAATG 2 cut(s) 390, 471
BseRI GAGGAG 1 cut(s) 71
BseSI GKGCMC 1 cut(s) 457
BseXI GCAGC 6 cut(s) 49, 52, 391, 427, 430, 473
BseYI CCCAGC 1 cut(s) 33
BshFI GGCC 3 cut(s) 77, 312, 469
BsiHKAI GWGCWC 2 cut(s) 250, 457
BsiSI CCGG 2 cut(s) 90, 188
BslFI GGGAC 1 cut(s) 113
BsmFI GGGAC 1 cut(s) 113
BsnI GGCC 3 cut(s) 77, 312, 469
Bsp1286I GDGCHC 2 cut(s) 250, 457
Bsp143I GATC 1 cut(s) 196
BspANI GGCC 3 cut(s) 77, 312, 469
BspLI GGNNCC 1 cut(s) 311
BspMAI CTGCAG 2 cut(s) 70, 463
BspMI ACCTGC 1 cut(s) 466
BsrDI GCAATG 2 cut(s) 390, 471
BssECI CCNNGG 1 cut(s) 88
BssMI GATC 1 cut(s) 196
Bst4CI ACNGT 2 cut(s) 15, 127
Bst6I CTCTTC 1 cut(s) 489
BstAPI GCANNNNNTGC 1 cut(s) 452
BstDEI CTNAG 2 cut(s) 208, 504
BstF5I GGATG 2 cut(s) 282, 297
BstH2I RGCGCY 1 cut(s) 208
BstHHI GCGC 1 cut(s) 207
BstKTI GATC 1 cut(s) 199
BstMBI GATC 1 cut(s) 196
BstMWI GCNNNNNNNGC 5 cut(s) 74, 179, 401, 452, 461
BstNSI RCATGY 2 cut(s) 23, 286
BstSCI CCNGG 1 cut(s) 88
BstSFI CTRYAG 2 cut(s) 66, 459
BstSLI GKGCMC 1 cut(s) 457
BstV1I GCAGC 6 cut(s) 49, 52, 391, 427, 430, 473
BstV2I GAAGAC 1 cut(s) 486
BsuRI GGCC 3 cut(s) 77, 312, 469
BtsCI GGATG 2 cut(s) 282, 297
BveI ACCTGC 1 cut(s) 466
CfoI GCGC 1 cut(s) 207
Cfr13I GGNCC 3 cut(s) 76, 310, 468
Csp6I GTAC 1 cut(s) 6
CviAII CATG 4 cut(s) 20, 121, 283, 385
CviQI GTAC 1 cut(s) 6
DdeI CTNAG 2 cut(s) 208, 504
DpnI GATC 1 cut(s) 198
DpnII GATC 1 cut(s) 196
Eam1104I CTCTTC 1 cut(s) 489
EarI CTCTTC 1 cut(s) 489
Ecl136II GAGCTC 1 cut(s) 248
Eco24I GRGCYC 1 cut(s) 250
Eco47III AGCGCT 1 cut(s) 206
Eco53kI GAGCTC 1 cut(s) 248
EcoICRI GAGCTC 1 cut(s) 248
EcoO109I RGGNCCY 2 cut(s) 76, 310
EcoRI GAATTC 1 cut(s) 258
EcoT38I GRGCYC 1 cut(s) 250
FaeI CATG 4 cut(s) 23, 124, 286, 388
FaiI YATR 7 cut(s) 21, 122, 159, 284, 386, 426, 431
FaqI GGGAC 1 cut(s) 113
FatI CATG 4 cut(s) 19, 120, 282, 384
FblI GTMKAC 1 cut(s) 375
Fnu4HI GCNGC 6 cut(s) 63, 66, 405, 441, 444, 462
FokI GGATG 2 cut(s) 289, 304
FriOI GRGCYC 1 cut(s) 250
Fsp4HI GCNGC 6 cut(s) 63, 66, 405, 441, 444, 462
FspBI CTAG 3 cut(s) 168, 218, 299
GlaI GCGC 1 cut(s) 206
GluI GCNGC 6 cut(s) 63, 66, 405, 441, 444, 462
GsaI CCCAGC 1 cut(s) 37
GsuI CTGGAG 2 cut(s) 185, 264
HaeII RGCGCY 1 cut(s) 208
HaeIII GGCC 3 cut(s) 77, 312, 469
HapII CCGG 2 cut(s) 90, 188
HhaI GCGC 1 cut(s) 207
Hin1II CATG 4 cut(s) 23, 124, 286, 388
Hin6I GCGC 1 cut(s) 205
HinP1I GCGC 1 cut(s) 205
HinfI GANTC 1 cut(s) 328
HpaII CCGG 2 cut(s) 90, 188
Hpy166II GTNNAC 4 cut(s) 6, 346, 376, 455
Hpy188I TCNGA 1 cut(s) 335
Hpy188III TCNNGA 1 cut(s) 289
Hpy8I GTNNAC 4 cut(s) 6, 346, 376, 455
HpyAV CCTTC 2 cut(s) 317, 430
HpyCH4III ACNGT 2 cut(s) 15, 127
HpyF10VI GCNNNNNNNGC 5 cut(s) 74, 179, 401, 452, 461
HpyF3I CTNAG 2 cut(s) 208, 504
Hsp92II CATG 4 cut(s) 23, 124, 286, 388
HspAI GCGC 1 cut(s) 205
Kzo9I GATC 1 cut(s) 196
LmnI GCTCC 2 cut(s) 92, 245
LpnPI CCDG 9 cut(s) 47, 72, 103, 201, 207, 215, 228, 292, 471
Lsp1109I GCAGC 6 cut(s) 49, 52, 391, 427, 430, 473
LweI GCATC 1 cut(s) 282
MaeI CTAG 3 cut(s) 168, 218, 299
MaeIII GTNAC 3 cut(s) 22, 190, 227
MalI GATC 1 cut(s) 198
MboI GATC 1 cut(s) 196
MboII GAAGA 4 cut(s) 21, 445, 476, 486
MhlI GDGCHC 2 cut(s) 250, 457
MluCI AATT 1 cut(s) 258
MmeI TCCRAC 1 cut(s) 116
MnlI CCTC 8 cut(s) 89, 92, 193, 205, 263, 267, 323, 492
MroXI GAANNNNTTC 2 cut(s) 54, 327
MseI TTAA 1 cut(s) 72
MspA1I CMGCKG 1 cut(s) 37
MspI CCGG 2 cut(s) 90, 188
MspR9I CCNGG 1 cut(s) 90
MwoI GCNNNNNNNGC 5 cut(s) 74, 179, 401, 452, 461
NciI CCSGG 1 cut(s) 90
NdeII GATC 1 cut(s) 196
NlaIII CATG 4 cut(s) 23, 124, 286, 388
NlaIV GGNNCC 1 cut(s) 311
NmuCI GTSAC 1 cut(s) 22
NspI RCATGY 2 cut(s) 23, 286
PaqCI CACCTGC 1 cut(s) 466
PcsI WCGNNNNNNNCGW 1 cut(s) 339
PdmI GAANNNNTTC 2 cut(s) 54, 327
PfeI GAWTC 1 cut(s) 328
PkrI GCNGC 6 cut(s) 64, 67, 406, 442, 445, 463
Psp124BI GAGCTC 1 cut(s) 250
PspFI CCCAGC 1 cut(s) 33
PspN4I GGNNCC 1 cut(s) 311
PspPI GGNCC 3 cut(s) 76, 310, 468
PstI CTGCAG 2 cut(s) 70, 463
PvuII CAGCTG 1 cut(s) 37
RsaI GTAC 1 cut(s) 7
RsaNI GTAC 1 cut(s) 6
SacI GAGCTC 1 cut(s) 250
SaqAI TTAA 1 cut(s) 72
SatI GCNGC 6 cut(s) 63, 66, 405, 441, 444, 462
Sau3AI GATC 1 cut(s) 196
Sau96I GGNCC 3 cut(s) 76, 310, 468
ScrFI CCNGG 1 cut(s) 90
SduI GDGCHC 2 cut(s) 250, 457
SfaNI GCATC 1 cut(s) 282
SfcI CTRYAG 2 cut(s) 66, 459
SpeI ACTAGT 1 cut(s) 167
Sse9I AATT 1 cut(s) 258
SspMI CTAG 3 cut(s) 168, 218, 299
SstI GAGCTC 1 cut(s) 250
StyD4I CCNGG 1 cut(s) 88
TaaI ACNGT 2 cut(s) 15, 127
TasI AATT 1 cut(s) 258
TfiI GAWTC 1 cut(s) 328
Tru1I TTAA 1 cut(s) 72
Tru9I TTAA 1 cut(s) 72
TseFI GTSAC 1 cut(s) 22
TseI GCWGC 6 cut(s) 62, 65, 404, 440, 443, 461
Tsp45I GTSAC 1 cut(s) 22
TspDTI ATGAA 1 cut(s) 446
VneI GTGCAC 1 cut(s) 453
XapI RAATTY 1 cut(s) 258
XceI RCATGY 2 cut(s) 23, 286
XmiI GTMKAC 1 cut(s) 375
XmnI GAANNNNTTC 2 cut(s) 54, 327
XspI CTAG 3 cut(s) 168, 218, 299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.