pycom17g20930

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0005492
Physical Location & Seq
Reverse (-)
19353054 .. 19354935
1882 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g20930.2

Sequence Viewer

Length: 588 bp
ATGGCTTCTTCAAAGAAATGGGCAACCACCATATCTTCCATTGCTTCTTTTATATATTTCCTTGTCATTATTCTTCAAATTCCTCTTTTCAGCATTCCCTGTACCGGCGGAATATGTACAACACCAGTAGAGGTAACATCCTCACAGTTAATTGCATCTGAAGTATTTCCTTTATTTCTAGTGAAGGCGCTACTCTACCCGGGGGCCTTTGCGAATGCATTCATCAAGAACAAAGCAATCCCAGGCTATGATAACTTGAAGTTATATAACTTCACCAATGTAAAGGCAGCTGATGCAATCTCTGACCTACAACGTCTAGAGGTTCTTGTTGGAAGCTACTTATCAGTGGCAGGAGCTTTTCTTGGTCTCATTAGACCAGGGAGAATGAGCCTCTTTGGTATGCTTCTCATCGTATGGGGGCTTGTAAGGGAAATCTTTTCGAGAAAATCAACGAGCACAGATCTTATATATATGTACCCAGCGATATCATTTCCCTTGTTTGCTGCCTTCTTGTCTATAACAAGAGATGTAAGGAGGATAATCCGTTGTTACAGAACTCCTCGATTCGTGAAAACAAATCTTATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

196

Amino Acids

21.68

Weight (kDa)

9.87

Isoelectric Point (pI)

31.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 108
AcsI RAATTY 1 cut(s) 78
AcuI CTGAAG 1 cut(s) 180
AfaI GTAC 3 cut(s) 103, 118, 476
AfiI CCNNNNNNNGG 1 cut(s) 104
AgsI TTSAA 3 cut(s) 12, 77, 259
AjnI CCWGG 2 cut(s) 241, 376
AluBI AGCT 3 cut(s) 290, 336, 356
AluI AGCT 3 cut(s) 290, 336, 356
Alw21I GWGCWC 1 cut(s) 458
Alw26I GTCTC 1 cut(s) 371
Ama87I CYCGRG 1 cut(s) 199
AoxI GGCC 1 cut(s) 204
ApeKI GCWGC 2 cut(s) 287, 503
ApoI RAATTY 1 cut(s) 78
Asp700I GAANNNNTTC 2 cut(s) 165, 218
AspLEI GCGC 1 cut(s) 190
AspS9I GGNCC 1 cut(s) 204
AsuC2I CCSGG 2 cut(s) 200, 201
AsuHPI GGTGA 1 cut(s) 265
AvaI CYCGRG 1 cut(s) 199
Bbv12I GWGCWC 1 cut(s) 458
BbvI GCAGC 2 cut(s) 299, 490
BciT130I CCWGG 2 cut(s) 243, 378
BcnI CCSGG 2 cut(s) 200, 201
BcoDI GTCTC 1 cut(s) 371
BfaI CTAG 2 cut(s) 179, 317
BfoI RGCGCY 1 cut(s) 191
BglII AGATCT 1 cut(s) 460
BisI GCNGC 2 cut(s) 288, 504
BlsI GCNGC 2 cut(s) 289, 505
Bme1390I CCNGG 4 cut(s) 200, 201, 243, 378
BmeT110I CYCGRG 1 cut(s) 199
BmgT120I GGNCC 1 cut(s) 204
BmiI GGNNCC 1 cut(s) 205
BmrFI CCNGG 4 cut(s) 200, 201, 243, 378
BmsI GCATC 2 cut(s) 164, 283
BpuMI CCSGG 2 cut(s) 200, 201
BsaI GGTCTC 1 cut(s) 371
BsaJI CCNNGG 4 cut(s) 199, 200, 241, 377
Bsc4I CCNNNNNNNGG 1 cut(s) 104
Bse118I RCCGGY 1 cut(s) 104
Bse1I ACTGG 1 cut(s) 125
Bse3DI GCAATG 1 cut(s) 39
BseBI CCWGG 2 cut(s) 243, 378
BseDI CCNNGG 4 cut(s) 199, 200, 241, 377
BseGI GGATG 1 cut(s) 137
BseLI CCNNNNNNNGG 1 cut(s) 104
BseMI GCAATG 1 cut(s) 39
BseNI ACTGG 1 cut(s) 125
BseRI GAGGAG 1 cut(s) 549
BseXI GCAGC 2 cut(s) 299, 490
BseYI CCCAGC 1 cut(s) 478
BshFI GGCC 1 cut(s) 206
BsiHKAI GWGCWC 1 cut(s) 458
BsiHKCI CYCGRG 1 cut(s) 199
BsiSI CCGG 2 cut(s) 105, 200
BslI CCNNNNNNNGG 1 cut(s) 104
BsmAI GTCTC 1 cut(s) 371
BsmI GAATGC 3 cut(s) 93, 218, 220
BsnI GGCC 1 cut(s) 206
Bso31I GGTCTC 1 cut(s) 371
BsoBI CYCGRG 1 cut(s) 199
Bsp1286I GDGCHC 1 cut(s) 458
Bsp1407I TGTACA 1 cut(s) 116
Bsp143I GATC 1 cut(s) 460
BspACI CCGC 1 cut(s) 108
BspANI GGCC 1 cut(s) 206
BspLI GGNNCC 1 cut(s) 205
BspTNI GGTCTC 1 cut(s) 371
BsrDI GCAATG 1 cut(s) 39
BsrFI RCCGGY 1 cut(s) 104
BsrGI TGTACA 1 cut(s) 116
BsrI ACTGG 1 cut(s) 125
BssAI RCCGGY 1 cut(s) 104
BssECI CCNNGG 4 cut(s) 199, 200, 241, 377
BssMI GATC 1 cut(s) 460
Bst2UI CCWGG 2 cut(s) 243, 378
Bst4CI ACNGT 1 cut(s) 147
BstAPI GCANNNNNTGC 1 cut(s) 293
BstAUI TGTACA 1 cut(s) 116
BstF5I GGATG 1 cut(s) 137
BstH2I RGCGCY 1 cut(s) 191
BstHHI GCGC 1 cut(s) 190
BstKTI GATC 1 cut(s) 463
BstMAI GTCTC 1 cut(s) 371
BstMBI GATC 1 cut(s) 460
BstMWI GCNNNNNNNGC 1 cut(s) 293
BstNI CCWGG 2 cut(s) 243, 378
BstSCI CCNGG 4 cut(s) 198, 199, 241, 376
BstV1I GCAGC 2 cut(s) 299, 490
BstX2I RGATCY 1 cut(s) 460
BstYI RGATCY 1 cut(s) 460
BsuRI GGCC 1 cut(s) 206
BtsCI GGATG 1 cut(s) 137
BtsIMutI CAGTG 1 cut(s) 351
CfoI GCGC 1 cut(s) 190
Cfr10I RCCGGY 1 cut(s) 104
Cfr13I GGNCC 1 cut(s) 204
Cfr9I CCCGGG 1 cut(s) 199
Csp6I GTAC 3 cut(s) 102, 117, 475
CviJI RGCY 8 cut(s) 5, 206, 246, 290, 336, 356, 390, 421
CviKI_1 RGCY 8 cut(s) 5, 206, 246, 290, 336, 356, 390, 421
CviQI GTAC 3 cut(s) 102, 117, 475
DpnI GATC 1 cut(s) 462
DpnII GATC 1 cut(s) 460
EciI GGCGGA 1 cut(s) 123
Eco31I GGTCTC 1 cut(s) 371
Eco32I GATATC 1 cut(s) 486
Eco57I CTGAAG 1 cut(s) 180
Eco88I CYCGRG 1 cut(s) 199
EcoO109I RGGNCCY 1 cut(s) 204
EcoRII CCWGG 2 cut(s) 241, 376
EcoRV GATATC 1 cut(s) 486
EcoT22I ATGCAT 1 cut(s) 220
Fnu4HI GCNGC 2 cut(s) 288, 504
FokI GGATG 1 cut(s) 124
Fsp4HI GCNGC 2 cut(s) 288, 504
FspBI CTAG 2 cut(s) 179, 317
GlaI GCGC 1 cut(s) 189
GluI GCNGC 2 cut(s) 288, 504
GsaI CCCAGC 1 cut(s) 482
HaeII RGCGCY 1 cut(s) 191
HaeIII GGCC 1 cut(s) 206
HapII CCGG 2 cut(s) 105, 200
HhaI GCGC 1 cut(s) 190
Hin6I GCGC 1 cut(s) 188
HinP1I GCGC 1 cut(s) 188
HinfI GANTC 1 cut(s) 564
HpaII CCGG 2 cut(s) 105, 200
HphI GGTGA 1 cut(s) 265
Hpy188I TCNGA 2 cut(s) 160, 304
Hpy188III TCNNGA 4 cut(s) 226, 317, 441, 568
HpyAV CCTTC 2 cut(s) 178, 517
HpyCH4III ACNGT 1 cut(s) 147
HpyCH4IV ACGT 1 cut(s) 313
HpyCH4V TGCA 3 cut(s) 155, 218, 296
HpyF10VI GCNNNNNNNGC 1 cut(s) 293
HpySE526I ACGT 1 cut(s) 313
HspAI GCGC 1 cut(s) 188
Kzo9I GATC 1 cut(s) 460
LmnI GCTCC 1 cut(s) 353
Lsp1109I GCAGC 2 cut(s) 299, 490
LweI GCATC 2 cut(s) 164, 283
MaeI CTAG 2 cut(s) 179, 317
MaeII ACGT 1 cut(s) 313
MaeIII GTNAC 2 cut(s) 133, 548
MalI GATC 1 cut(s) 462
MboI GATC 1 cut(s) 460
MboII GAAGA 2 cut(s) 27, 65
MflI RGATCY 1 cut(s) 460
MhlI GDGCHC 1 cut(s) 458
MluCI AATT 2 cut(s) 78, 150
MmeI TCCRAC 1 cut(s) 310
MnlI CCTC 7 cut(s) 93, 124, 151, 313, 401, 528, 570
Mph1103I ATGCAT 1 cut(s) 220
MroXI GAANNNNTTC 2 cut(s) 165, 218
MseI TTAA 1 cut(s) 149
MspA1I CMGCKG 1 cut(s) 290
MspI CCGG 2 cut(s) 105, 200
MspR9I CCNGG 4 cut(s) 200, 201, 243, 378
Mva1269I GAATGC 3 cut(s) 93, 218, 220
MvaI CCWGG 2 cut(s) 243, 378
MwoI GCNNNNNNNGC 1 cut(s) 293
NciI CCSGG 2 cut(s) 200, 201
NdeII GATC 1 cut(s) 460
NlaIV GGNNCC 1 cut(s) 205
NsiI ATGCAT 1 cut(s) 220
PctI GAATGC 3 cut(s) 93, 218, 220
PdmI GAANNNNTTC 2 cut(s) 165, 218
PfeI GAWTC 1 cut(s) 564
PkrI GCNGC 2 cut(s) 289, 505
Psp6I CCWGG 2 cut(s) 241, 376
PspFI CCCAGC 1 cut(s) 478
PspGI CCWGG 2 cut(s) 241, 376
PspN4I GGNNCC 1 cut(s) 205
PspPI GGNCC 1 cut(s) 204
PsuI RGATCY 1 cut(s) 460
PvuII CAGCTG 1 cut(s) 290
RsaI GTAC 3 cut(s) 103, 118, 476
RsaNI GTAC 3 cut(s) 102, 117, 475
SaqAI TTAA 1 cut(s) 149
SatI GCNGC 2 cut(s) 288, 504
Sau3AI GATC 1 cut(s) 460
Sau96I GGNCC 1 cut(s) 204
ScrFI CCNGG 4 cut(s) 200, 201, 243, 378
SduI GDGCHC 1 cut(s) 458
SetI ASST 7 cut(s) 135, 292, 309, 316, 324, 338, 358
SfaNI GCATC 2 cut(s) 164, 283
SmaI CCCGGG 1 cut(s) 201
Sse9I AATT 2 cut(s) 78, 150
SsiI CCGC 1 cut(s) 108
SspMI CTAG 2 cut(s) 179, 317
StyD4I CCNGG 4 cut(s) 198, 199, 241, 376
TaaI ACNGT 1 cut(s) 147
TaiI ACGT 1 cut(s) 316
TaqI TCGA 2 cut(s) 440, 562
TasI AATT 2 cut(s) 78, 150
TatI WGTACW 1 cut(s) 116
TfiI GAWTC 1 cut(s) 564
Tru1I TTAA 1 cut(s) 149
Tru9I TTAA 1 cut(s) 149
TscAI CASTG 1 cut(s) 351
TseI GCWGC 2 cut(s) 287, 503
TspDTI ATGAA 1 cut(s) 211
TspGWI ACGGA 1 cut(s) 533
TspMI CCCGGG 1 cut(s) 199
TspRI CASTG 1 cut(s) 351
XapI RAATTY 1 cut(s) 78
XbaI TCTAGA 1 cut(s) 316
XmaI CCCGGG 1 cut(s) 199
XmnI GAANNNNTTC 2 cut(s) 165, 218
XspI CTAG 2 cut(s) 179, 317
Zsp2I ATGCAT 1 cut(s) 220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.