RLG00000019537

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0005492
Physical Location & Seq
Forward (+)
53289306 .. 53291304
1999 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019537

Sequence Viewer

Length: 588 bp
ATGGCGTCTTCAAGGAAATGGGCAACCACCATATCCTTCGCTGCTTCGTGTATCTATTTCACTGTCATCATCATTCAGATTCCTCTTTTCAGTGTCCCATGTAGATCTGGAACATGTACATCACCAATAGCAGTCACAGCCCCTCAGCTGATGGCATGTGATCTGTTTCCTTTAGTTGCAATAAAAGCACTAGTCTACCCGGGTGCCATTGCAAATGCATTAGTGAAAAATGAAGCAATTCCAAGCTATAGTAACTTGAAGTTACATGACCCCTCTGCTGTAAAGGCAGCTGATGCAATTGGTGATCTGCAGCGCCTAGAGGTTCTGGTTGGAAGCTACTTGTCAGTCGCTGGAGCATTCTGTGGACTTATAAAACCATGGAGAATGAGTTTCTATGGAGTGCTTCTTATCCTATGGGGCCTTGTTAAGGAAATTATATTGAGCAAATCAGCTAGTCCAAATCTTATATACATCCACCCAGCAATGTCAATCGCCGTCTGTTCTGCCTTCTTGTCACTAGCTAAGGATGTAAGGAGGATAATTCGAAGTTGCAGAGGTCTTCAGATTGTAAAAGAAAAACGTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

196

Amino Acids

21.09

Weight (kDa)

9.56

Isoelectric Point (pI)

47.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 371
AccB1I GGYRCC 1 cut(s) 203
AccI GTMKAC 1 cut(s) 195
AclI AACGTT 1 cut(s) 580
AcuI CTGAAG 1 cut(s) 545
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 118
AfiI CCNNNNNNNGG 1 cut(s) 427
AflIII ACRYGT 1 cut(s) 113
AgsI TTSAA 2 cut(s) 12, 259
AhlI ACTAGT 1 cut(s) 190
AluBI AGCT 6 cut(s) 148, 246, 290, 336, 452, 521
AluI AGCT 6 cut(s) 148, 246, 290, 336, 452, 521
AlwNI CAGNNNCTG 1 cut(s) 350
Ama87I CYCGRG 1 cut(s) 199
AoxI GGCC 1 cut(s) 418
ApeKI GCWGC 3 cut(s) 41, 287, 310
Asp700I GAANNNNTTC 1 cut(s) 237
AspLEI GCGC 1 cut(s) 315
AspS9I GGNCC 1 cut(s) 418
AsuC2I CCSGG 2 cut(s) 200, 201
AsuHPI GGTGA 2 cut(s) 114, 314
AsuII TTCGAA 1 cut(s) 544
AvaI CYCGRG 1 cut(s) 199
BanI GGYRCC 1 cut(s) 203
BbsI GAAGAC 1 cut(s) 551
BbvCI CCTCAGC 1 cut(s) 144
BbvI GCAGC 3 cut(s) 28, 299, 322
BccI CCATC 1 cut(s) 145
BceAI ACGGC 1 cut(s) 479
BcnI CCSGG 2 cut(s) 200, 201
BcuI ACTAGT 1 cut(s) 190
BfaI CTAG 4 cut(s) 191, 317, 453, 518
BfmI CTRYAG 2 cut(s) 247, 308
BfoI RGCGCY 1 cut(s) 316
BglII AGATCT 1 cut(s) 104
BisI GCNGC 3 cut(s) 42, 288, 311
BlsI GCNGC 3 cut(s) 43, 289, 312
Bme1390I CCNGG 2 cut(s) 200, 201
BmeT110I CYCGRG 1 cut(s) 199
BmgT120I GGNCC 1 cut(s) 418
BmiI GGNNCC 2 cut(s) 205, 419
BmrFI CCNGG 2 cut(s) 200, 201
BmsI GCATC 1 cut(s) 283
BpiI GAAGAC 1 cut(s) 551
BpmI CTGGAG 1 cut(s) 372
Bpu10I CCTNAGC 2 cut(s) 144, 522
Bpu14I TTCGAA 1 cut(s) 544
BpuMI CCSGG 2 cut(s) 200, 201
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 2 cut(s) 199, 377
BsaXI ACNNNNNCTCC 2 cut(s) 373, 403
Bsc4I CCNNNNNNNGG 1 cut(s) 427
Bse3DI GCAATG 2 cut(s) 207, 489
BseDI CCNNGG 2 cut(s) 199, 377
BseGI GGATG 2 cut(s) 471, 532
BseLI CCNNNNNNNGG 1 cut(s) 427
BseMI GCAATG 2 cut(s) 207, 489
BseMII CTCAG 1 cut(s) 158
BseXI GCAGC 3 cut(s) 28, 299, 322
BseYI CCCAGC 1 cut(s) 478
BshFI GGCC 1 cut(s) 420
BshNI GGYRCC 1 cut(s) 203
BsiHKCI CYCGRG 1 cut(s) 199
BsiSI CCGG 1 cut(s) 200
BslFI GGGAC 1 cut(s) 80
BslI CCNNNNNNNGG 1 cut(s) 427
BsmFI GGGAC 1 cut(s) 80
BsmI GAATGC 1 cut(s) 356
BsnI GGCC 1 cut(s) 420
BsoBI CYCGRG 1 cut(s) 199
Bsp119I TTCGAA 1 cut(s) 544
Bsp1407I TGTACA 1 cut(s) 116
Bsp143I GATC 3 cut(s) 104, 160, 304
Bsp19I CCATGG 1 cut(s) 377
BspANI GGCC 1 cut(s) 420
BspCNI CTCAG 1 cut(s) 157
BspLI GGNNCC 2 cut(s) 205, 419
BspMAI CTGCAG 1 cut(s) 312
BspT104I TTCGAA 1 cut(s) 544
BspT107I GGYRCC 1 cut(s) 203
BsrDI GCAATG 2 cut(s) 207, 489
BsrGI TGTACA 1 cut(s) 116
BssECI CCNNGG 2 cut(s) 199, 377
BssMI GATC 3 cut(s) 104, 160, 304
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 377
Bst4CI ACNGT 1 cut(s) 64
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 293
BstAUI TGTACA 1 cut(s) 116
BstBI TTCGAA 1 cut(s) 544
BstDEI CTNAG 2 cut(s) 144, 522
BstDSI CCRYGG 1 cut(s) 377
BstENI CCTNNNNNAGG 1 cut(s) 425
BstF5I GGATG 2 cut(s) 471, 532
BstH2I RGCGCY 1 cut(s) 316
BstHHI GCGC 1 cut(s) 315
BstKTI GATC 3 cut(s) 107, 163, 307
BstMBI GATC 3 cut(s) 104, 160, 304
BstMWI GCNNNNNNNGC 4 cut(s) 137, 185, 284, 293
BstNSI RCATGY 2 cut(s) 117, 159
BstSCI CCNGG 2 cut(s) 198, 199
BstSFI CTRYAG 2 cut(s) 247, 308
BstV1I GCAGC 3 cut(s) 28, 299, 322
BstV2I GAAGAC 1 cut(s) 551
BstX2I RGATCY 1 cut(s) 104
BstYI RGATCY 1 cut(s) 104
BsuRI GGCC 1 cut(s) 420
BtgI CCRYGG 1 cut(s) 377
BtsCI GGATG 2 cut(s) 471, 532
BtsIMutI CAGTG 2 cut(s) 60, 97
CaiI CAGNNNCTG 1 cut(s) 350
CfoI GCGC 1 cut(s) 315
Cfr13I GGNCC 1 cut(s) 418
Cfr9I CCCGGG 1 cut(s) 199
Csp6I GTAC 1 cut(s) 117
CviAII CATG 5 cut(s) 99, 114, 156, 266, 378
CviJI RGCY 8 cut(s) 140, 148, 246, 290, 336, 420, 452, 521
CviKI_1 RGCY 8 cut(s) 140, 148, 246, 290, 336, 420, 452, 521
CviQI GTAC 1 cut(s) 117
DdeI CTNAG 2 cut(s) 144, 522
DpnI GATC 3 cut(s) 106, 162, 306
DpnII GATC 3 cut(s) 104, 160, 304
Eco130I CCWWGG 1 cut(s) 377
Eco57I CTGAAG 1 cut(s) 545
Eco88I CYCGRG 1 cut(s) 199
EcoNI CCTNNNNNAGG 1 cut(s) 425
EcoO109I RGGNCCY 1 cut(s) 418
EcoT14I CCWWGG 1 cut(s) 377
EcoT22I ATGCAT 1 cut(s) 220
ErhI CCWWGG 1 cut(s) 377
FaeI CATG 5 cut(s) 102, 117, 159, 269, 381
FaqI GGGAC 1 cut(s) 80
FatI CATG 5 cut(s) 98, 113, 155, 265, 377
FblI GTMKAC 1 cut(s) 195
Fnu4HI GCNGC 3 cut(s) 42, 288, 311
FokI GGATG 2 cut(s) 458, 539
Fsp4HI GCNGC 3 cut(s) 42, 288, 311
FspBI CTAG 4 cut(s) 191, 317, 453, 518
GlaI GCGC 1 cut(s) 314
GluI GCNGC 3 cut(s) 42, 288, 311
GsaI CCCAGC 1 cut(s) 482
GsuI CTGGAG 1 cut(s) 372
HaeII RGCGCY 1 cut(s) 316
HaeIII GGCC 1 cut(s) 420
HapII CCGG 1 cut(s) 200
HhaI GCGC 1 cut(s) 315
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 5 cut(s) 102, 117, 159, 269, 381
Hin6I GCGC 1 cut(s) 313
HinP1I GCGC 1 cut(s) 313
HinfI GANTC 1 cut(s) 79
HpaII CCGG 1 cut(s) 200
HphI GGTGA 2 cut(s) 114, 314
Hpy166II GTNNAC 2 cut(s) 196, 365
Hpy188I TCNGA 2 cut(s) 78, 564
Hpy188III TCNNGA 1 cut(s) 108
Hpy8I GTNNAC 2 cut(s) 196, 365
HpyAV CCTTC 2 cut(s) 46, 517
HpyCH4III ACNGT 1 cut(s) 64
HpyCH4IV ACGT 1 cut(s) 580
HpyCH4V TGCA 6 cut(s) 179, 212, 218, 296, 310, 552
HpyF10VI GCNNNNNNNGC 4 cut(s) 137, 185, 284, 293
HpyF3I CTNAG 2 cut(s) 144, 522
HpySE526I ACGT 1 cut(s) 580
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 5 cut(s) 102, 117, 159, 269, 381
HspAI GCGC 1 cut(s) 313
Kzo9I GATC 3 cut(s) 104, 160, 304
LmnI GCTCC 1 cut(s) 353
LpnPI CCDG 5 cut(s) 93, 213, 311, 336, 492
Lsp1109I GCAGC 3 cut(s) 28, 299, 322
LweI GCATC 1 cut(s) 283
MaeI CTAG 4 cut(s) 191, 317, 453, 518
MaeII ACGT 1 cut(s) 580
MaeIII GTNAC 4 cut(s) 133, 251, 261, 513
MalI GATC 3 cut(s) 106, 162, 306
MboI GATC 3 cut(s) 104, 160, 304
MboII GAAGA 1 cut(s) 551
MfeI CAATTG 1 cut(s) 297
MflI RGATCY 1 cut(s) 104
MluCI AATT 4 cut(s) 237, 297, 432, 540
MmeI TCCRAC 1 cut(s) 310
MnlI CCTC 6 cut(s) 93, 153, 283, 313, 528, 548
Mph1103I ATGCAT 1 cut(s) 220
MroXI GAANNNNTTC 1 cut(s) 237
MseI TTAA 1 cut(s) 426
MspA1I CMGCKG 2 cut(s) 148, 290
MspI CCGG 1 cut(s) 200
MspR9I CCNGG 2 cut(s) 200, 201
MunI CAATTG 1 cut(s) 297
Mva1269I GAATGC 1 cut(s) 356
MwoI GCNNNNNNNGC 4 cut(s) 137, 185, 284, 293
NciI CCSGG 2 cut(s) 200, 201
NcoI CCATGG 1 cut(s) 377
NdeII GATC 3 cut(s) 104, 160, 304
NlaIII CATG 5 cut(s) 102, 117, 159, 269, 381
NlaIV GGNNCC 2 cut(s) 205, 419
NmuCI GTSAC 2 cut(s) 133, 513
NsiI ATGCAT 1 cut(s) 220
NspI RCATGY 2 cut(s) 117, 159
NspV TTCGAA 1 cut(s) 544
PciI ACATGT 1 cut(s) 113
PctI GAATGC 1 cut(s) 356
PdmI GAANNNNTTC 1 cut(s) 237
PfeI GAWTC 1 cut(s) 79
PkrI GCNGC 3 cut(s) 43, 289, 312
PscI ACATGT 1 cut(s) 113
PsiI TTATAA 1 cut(s) 371
Psp1406I AACGTT 1 cut(s) 580
PspFI CCCAGC 1 cut(s) 478
PspN4I GGNNCC 2 cut(s) 205, 419
PspPI GGNCC 1 cut(s) 418
PstI CTGCAG 1 cut(s) 312
PstNI CAGNNNCTG 1 cut(s) 350
PsuI RGATCY 1 cut(s) 104
PvuII CAGCTG 2 cut(s) 148, 290
RsaI GTAC 1 cut(s) 118
RsaNI GTAC 1 cut(s) 117
SaqAI TTAA 1 cut(s) 426
SatI GCNGC 3 cut(s) 42, 288, 311
Sau3AI GATC 3 cut(s) 104, 160, 304
Sau96I GGNCC 1 cut(s) 418
ScrFI CCNGG 2 cut(s) 200, 201
SetI ASST 9 cut(s) 150, 248, 292, 324, 338, 454, 523, 559, 583
SfaNI GCATC 1 cut(s) 283
SfcI CTRYAG 2 cut(s) 247, 308
SfuI TTCGAA 1 cut(s) 544
SmaI CCCGGG 1 cut(s) 201
SpeI ACTAGT 1 cut(s) 190
Sse9I AATT 4 cut(s) 237, 297, 432, 540
SspMI CTAG 4 cut(s) 191, 317, 453, 518
StyD4I CCNGG 2 cut(s) 198, 199
StyI CCWWGG 1 cut(s) 377
TaaI ACNGT 1 cut(s) 64
TaiI ACGT 1 cut(s) 583
TaqI TCGA 1 cut(s) 544
TasI AATT 4 cut(s) 237, 297, 432, 540
TatI WGTACW 1 cut(s) 116
TfiI GAWTC 1 cut(s) 79
Tru1I TTAA 1 cut(s) 426
Tru9I TTAA 1 cut(s) 426
TscAI CASTG 2 cut(s) 67, 97
TseFI GTSAC 2 cut(s) 133, 513
TseI GCWGC 3 cut(s) 41, 287, 310
Tsp45I GTSAC 2 cut(s) 133, 513
TspDTI ATGAA 1 cut(s) 246
TspMI CCCGGG 1 cut(s) 199
TspRI CASTG 2 cut(s) 67, 97
XagI CCTNNNNNAGG 1 cut(s) 425
XceI RCATGY 2 cut(s) 117, 159
XmaI CCCGGG 1 cut(s) 199
XmiI GTMKAC 1 cut(s) 195
XmnI GAANNNNTTC 1 cut(s) 237
XspI CTAG 4 cut(s) 191, 317, 453, 518
Zsp2I ATGCAT 1 cut(s) 220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.