Rroxscaffold_2G00108220

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0005492
Physical Location & Seq
Reverse (-)
32201725 .. 32204018
2294 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00108220.1

Sequence Viewer

Length: 654 bp
ATGGCCTCTTCAAGGAAATGGGCAACCACCATATCCTTCGCTGCTTCGTGTATCTATTTCACTGTCATCATCATTCAGATTCCTCTTTTCAGTGCTCAGTTTGCTTTGCAAAAACCAGATGTGGGTTTTGTTCTGATTGGCCAAATTGTGCAAGCTGAGCTCATTTGTTTATCTGGAACATGTACATCACCAATAGCGGTCACAGCCCCTCAGCTGATGGCATGTGATCTGTTTCCTTTAGTTGCAATAAAAGCACTAGTCTACCCGGGTGTCATTGCAAATGCATTGGTGAAAAATGAAGCAATCCCAAGCTATAGTAACTTGAAGTTACATGACCCCTCTGCTGTAAAGGCAGCTGATGCAATTGCTGATCTCCAGCGCCTAGAGGTTCTGGTTGGAAGCTACTTGTCAGTCGCTGGAGCATTCTGTGGACTTATAAAACCATGGAGAATGAGTTTCTATGGAGTGCTTCTTATCCTATGGGGCCTTGTTAAGGAAATTATATTGAGCAAATCAGCTAGTCCAAATCTTATATACATCCACCCAGCAATGTCAATTGCCGTCTGTTCTGCCTTCTTGTCTCTAGCTAAGGATGTAAGGAGGATAATTCGAAGTTGCAGAGGTCTTCAGATTGTAAAAGAAAAACGTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

217

Amino Acids

23.47

Weight (kDa)

9.34

Isoelectric Point (pI)

41.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 437
AccI GTMKAC 1 cut(s) 261
AciI CCGC 1 cut(s) 197
AclI AACGTT 1 cut(s) 646
AcoI YGGCCR 1 cut(s) 139
AcuI CTGAAG 1 cut(s) 611
AfaI GTAC 1 cut(s) 184
AfiI CCNNNNNNNGG 3 cut(s) 12, 122, 493
AflIII ACRYGT 1 cut(s) 179
AgsI TTSAA 2 cut(s) 12, 325
AhlI ACTAGT 1 cut(s) 256
AluBI AGCT 8 cut(s) 155, 160, 214, 312, 356, 402, 518, 587
AluI AGCT 8 cut(s) 155, 160, 214, 312, 356, 402, 518, 587
Alw21I GWGCWC 2 cut(s) 97, 162
Alw26I GTCTC 1 cut(s) 585
AlwNI CAGNNNCTG 1 cut(s) 416
Ama87I CYCGRG 1 cut(s) 265
AoxI GGCC 3 cut(s) 3, 139, 484
ApeKI GCWGC 2 cut(s) 41, 353
AspLEI GCGC 1 cut(s) 381
AspS9I GGNCC 1 cut(s) 484
AsuC2I CCSGG 2 cut(s) 266, 267
AsuHPI GGTGA 2 cut(s) 180, 301
AsuII TTCGAA 1 cut(s) 610
AvaI CYCGRG 1 cut(s) 265
BalI TGGCCA 1 cut(s) 141
BanII GRGCYC 1 cut(s) 162
BbsI GAAGAC 1 cut(s) 617
Bbv12I GWGCWC 2 cut(s) 97, 162
BbvCI CCTCAGC 1 cut(s) 210
BbvI GCAGC 2 cut(s) 28, 365
BccI CCATC 1 cut(s) 211
BceAI ACGGC 1 cut(s) 545
BcnI CCSGG 2 cut(s) 266, 267
BcoDI GTCTC 1 cut(s) 585
BcuI ACTAGT 1 cut(s) 256
BfaI CTAG 4 cut(s) 257, 383, 519, 584
BfmI CTRYAG 1 cut(s) 313
BfoI RGCGCY 1 cut(s) 382
BisI GCNGC 2 cut(s) 42, 354
BlpI GCTNAGC 1 cut(s) 156
BlsI GCNGC 2 cut(s) 43, 355
Bme1390I CCNGG 2 cut(s) 266, 267
BmeT110I CYCGRG 1 cut(s) 265
BmgT120I GGNCC 1 cut(s) 484
BmiI GGNNCC 1 cut(s) 485
BmrFI CCNGG 2 cut(s) 266, 267
BmsI GCATC 1 cut(s) 349
BpiI GAAGAC 1 cut(s) 617
BpmI CTGGAG 2 cut(s) 359, 438
Bpu10I CCTNAGC 2 cut(s) 210, 588
Bpu1102I GCTNAGC 1 cut(s) 156
Bpu14I TTCGAA 1 cut(s) 610
BpuMI CCSGG 2 cut(s) 266, 267
BsaJI CCNNGG 2 cut(s) 265, 443
BsaXI ACNNNNNCTCC 2 cut(s) 439, 469
Bsc4I CCNNNNNNNGG 3 cut(s) 12, 122, 493
Bse3DI GCAATG 2 cut(s) 273, 555
BseDI CCNNGG 2 cut(s) 265, 443
BseGI GGATG 2 cut(s) 537, 598
BseLI CCNNNNNNNGG 3 cut(s) 12, 122, 493
BseMI GCAATG 2 cut(s) 273, 555
BseMII CTCAG 3 cut(s) 110, 147, 224
BseXI GCAGC 2 cut(s) 28, 365
BseYI CCCAGC 1 cut(s) 544
BshFI GGCC 3 cut(s) 5, 141, 486
BsiHKAI GWGCWC 2 cut(s) 97, 162
BsiHKCI CYCGRG 1 cut(s) 265
BsiSI CCGG 1 cut(s) 266
BslI CCNNNNNNNGG 3 cut(s) 12, 122, 493
BsmAI GTCTC 1 cut(s) 585
BsmI GAATGC 1 cut(s) 422
BsnI GGCC 3 cut(s) 5, 141, 486
BsoBI CYCGRG 1 cut(s) 265
Bsp119I TTCGAA 1 cut(s) 610
Bsp1286I GDGCHC 2 cut(s) 97, 162
Bsp1407I TGTACA 1 cut(s) 182
Bsp143I GATC 2 cut(s) 226, 370
Bsp1720I GCTNAGC 1 cut(s) 156
Bsp19I CCATGG 1 cut(s) 443
BspACI CCGC 1 cut(s) 197
BspANI GGCC 3 cut(s) 5, 141, 486
BspCNI CTCAG 3 cut(s) 109, 148, 223
BspLI GGNNCC 1 cut(s) 485
BspT104I TTCGAA 1 cut(s) 610
BsrDI GCAATG 2 cut(s) 273, 555
BsrGI TGTACA 1 cut(s) 182
BssECI CCNNGG 2 cut(s) 265, 443
BssMI GATC 2 cut(s) 226, 370
BssT1I CCWWGG 1 cut(s) 443
Bst4CI ACNGT 1 cut(s) 64
Bst6I CTCTTC 1 cut(s) 13
BstAPI GCANNNNNTGC 1 cut(s) 359
BstAUI TGTACA 1 cut(s) 182
BstBI TTCGAA 1 cut(s) 610
BstC8I GCNNGC 1 cut(s) 153
BstDEI CTNAG 4 cut(s) 96, 156, 210, 588
BstDSI CCRYGG 1 cut(s) 443
BstENI CCTNNNNNAGG 2 cut(s) 10, 491
BstF5I GGATG 2 cut(s) 537, 598
BstH2I RGCGCY 1 cut(s) 382
BstHHI GCGC 1 cut(s) 381
BstKTI GATC 2 cut(s) 229, 373
BstMAI GTCTC 1 cut(s) 585
BstMBI GATC 2 cut(s) 226, 370
BstMWI GCNNNNNNNGC 6 cut(s) 101, 157, 203, 251, 350, 359
BstNSI RCATGY 2 cut(s) 183, 225
BstSCI CCNGG 2 cut(s) 264, 265
BstSFI CTRYAG 1 cut(s) 313
BstV1I GCAGC 2 cut(s) 28, 365
BstV2I GAAGAC 1 cut(s) 617
BsuRI GGCC 3 cut(s) 5, 141, 486
BtgI CCRYGG 1 cut(s) 443
BtsCI GGATG 2 cut(s) 537, 598
BtsIMutI CAGTG 2 cut(s) 60, 97
Cac8I GCNNGC 1 cut(s) 153
CaiI CAGNNNCTG 1 cut(s) 416
CfoI GCGC 1 cut(s) 381
Cfr13I GGNCC 1 cut(s) 484
Cfr9I CCCGGG 1 cut(s) 265
Csp6I GTAC 1 cut(s) 183
CviAII CATG 4 cut(s) 180, 222, 332, 444
CviQI GTAC 1 cut(s) 183
DdeI CTNAG 4 cut(s) 96, 156, 210, 588
DpnI GATC 2 cut(s) 228, 372
DpnII GATC 2 cut(s) 226, 370
EaeI YGGCCR 1 cut(s) 139
Eam1104I CTCTTC 1 cut(s) 13
EarI CTCTTC 1 cut(s) 13
Ecl136II GAGCTC 1 cut(s) 160
Eco130I CCWWGG 1 cut(s) 443
Eco24I GRGCYC 1 cut(s) 162
Eco53kI GAGCTC 1 cut(s) 160
Eco57I CTGAAG 1 cut(s) 611
Eco88I CYCGRG 1 cut(s) 265
EcoICRI GAGCTC 1 cut(s) 160
EcoNI CCTNNNNNAGG 2 cut(s) 10, 491
EcoO109I RGGNCCY 1 cut(s) 484
EcoT14I CCWWGG 1 cut(s) 443
EcoT22I ATGCAT 1 cut(s) 286
EcoT38I GRGCYC 1 cut(s) 162
ErhI CCWWGG 1 cut(s) 443
FaeI CATG 4 cut(s) 183, 225, 335, 447
FatI CATG 4 cut(s) 179, 221, 331, 443
FblI GTMKAC 1 cut(s) 261
Fnu4HI GCNGC 2 cut(s) 42, 354
FokI GGATG 2 cut(s) 524, 605
FriOI GRGCYC 1 cut(s) 162
Fsp4HI GCNGC 2 cut(s) 42, 354
FspBI CTAG 4 cut(s) 257, 383, 519, 584
GlaI GCGC 1 cut(s) 380
GluI GCNGC 2 cut(s) 42, 354
GsaI CCCAGC 1 cut(s) 548
GsuI CTGGAG 2 cut(s) 359, 438
HaeII RGCGCY 1 cut(s) 382
HaeIII GGCC 3 cut(s) 5, 141, 486
HapII CCGG 1 cut(s) 266
HhaI GCGC 1 cut(s) 381
Hin1II CATG 4 cut(s) 183, 225, 335, 447
Hin6I GCGC 1 cut(s) 379
HinP1I GCGC 1 cut(s) 379
HinfI GANTC 1 cut(s) 79
HpaII CCGG 1 cut(s) 266
HphI GGTGA 2 cut(s) 180, 301
Hpy166II GTNNAC 2 cut(s) 262, 431
Hpy188I TCNGA 3 cut(s) 78, 135, 630
Hpy188III TCNNGA 1 cut(s) 174
Hpy8I GTNNAC 2 cut(s) 262, 431
HpyAV CCTTC 2 cut(s) 46, 583
HpyCH4III ACNGT 1 cut(s) 64
HpyCH4IV ACGT 1 cut(s) 646
HpyCH4V TGCA 7 cut(s) 109, 151, 245, 278, 284, 362, 618
HpyF10VI GCNNNNNNNGC 6 cut(s) 101, 157, 203, 251, 350, 359
HpyF3I CTNAG 4 cut(s) 96, 156, 210, 588
HpySE526I ACGT 1 cut(s) 646
Hsp92II CATG 4 cut(s) 183, 225, 335, 447
HspAI GCGC 1 cut(s) 379
Kzo9I GATC 2 cut(s) 226, 370
LmnI GCTCC 1 cut(s) 419
LpnPI CCDG 7 cut(s) 129, 159, 279, 377, 389, 402, 558
Lsp1109I GCAGC 2 cut(s) 28, 365
LweI GCATC 1 cut(s) 349
MaeI CTAG 4 cut(s) 257, 383, 519, 584
MaeII ACGT 1 cut(s) 646
MaeIII GTNAC 3 cut(s) 199, 317, 327
MalI GATC 2 cut(s) 228, 372
MboI GATC 2 cut(s) 226, 370
MboII GAAGA 1 cut(s) 617
MfeI CAATTG 2 cut(s) 363, 555
MhlI GDGCHC 2 cut(s) 97, 162
MlsI TGGCCA 1 cut(s) 141
MluCI AATT 5 cut(s) 144, 363, 498, 555, 606
MluNI TGGCCA 1 cut(s) 141
MmeI TCCRAC 1 cut(s) 376
MnlI CCTC 7 cut(s) 16, 93, 219, 349, 379, 594, 614
Mox20I TGGCCA 1 cut(s) 141
Mph1103I ATGCAT 1 cut(s) 286
MscI TGGCCA 1 cut(s) 141
MseI TTAA 1 cut(s) 492
Msp20I TGGCCA 1 cut(s) 141
MspA1I CMGCKG 2 cut(s) 214, 356
MspI CCGG 1 cut(s) 266
MspR9I CCNGG 2 cut(s) 266, 267
MunI CAATTG 2 cut(s) 363, 555
Mva1269I GAATGC 1 cut(s) 422
MwoI GCNNNNNNNGC 6 cut(s) 101, 157, 203, 251, 350, 359
NciI CCSGG 2 cut(s) 266, 267
NcoI CCATGG 1 cut(s) 443
NdeII GATC 2 cut(s) 226, 370
NlaIII CATG 4 cut(s) 183, 225, 335, 447
NlaIV GGNNCC 1 cut(s) 485
NmuCI GTSAC 1 cut(s) 199
NsiI ATGCAT 1 cut(s) 286
NspI RCATGY 2 cut(s) 183, 225
NspV TTCGAA 1 cut(s) 610
PciI ACATGT 1 cut(s) 179
PctI GAATGC 1 cut(s) 422
PfeI GAWTC 1 cut(s) 79
PkrI GCNGC 2 cut(s) 43, 355
PscI ACATGT 1 cut(s) 179
PsiI TTATAA 1 cut(s) 437
Psp124BI GAGCTC 1 cut(s) 162
Psp1406I AACGTT 1 cut(s) 646
PspFI CCCAGC 1 cut(s) 544
PspN4I GGNNCC 1 cut(s) 485
PspPI GGNCC 1 cut(s) 484
PstNI CAGNNNCTG 1 cut(s) 416
PvuII CAGCTG 2 cut(s) 214, 356
RsaI GTAC 1 cut(s) 184
RsaNI GTAC 1 cut(s) 183
SacI GAGCTC 1 cut(s) 162
SaqAI TTAA 1 cut(s) 492
SatI GCNGC 2 cut(s) 42, 354
Sau3AI GATC 2 cut(s) 226, 370
Sau96I GGNCC 1 cut(s) 484
ScrFI CCNGG 2 cut(s) 266, 267
SduI GDGCHC 2 cut(s) 97, 162
SfaNI GCATC 1 cut(s) 349
SfcI CTRYAG 1 cut(s) 313
SfuI TTCGAA 1 cut(s) 610
SmaI CCCGGG 1 cut(s) 267
SpeI ACTAGT 1 cut(s) 256
Sse9I AATT 5 cut(s) 144, 363, 498, 555, 606
SsiI CCGC 1 cut(s) 197
SspMI CTAG 4 cut(s) 257, 383, 519, 584
SstI GAGCTC 1 cut(s) 162
StyD4I CCNGG 2 cut(s) 264, 265
StyI CCWWGG 1 cut(s) 443
TaaI ACNGT 1 cut(s) 64
TaiI ACGT 1 cut(s) 649
TaqI TCGA 1 cut(s) 610
TasI AATT 5 cut(s) 144, 363, 498, 555, 606
TatI WGTACW 1 cut(s) 182
TfiI GAWTC 1 cut(s) 79
Tru1I TTAA 1 cut(s) 492
Tru9I TTAA 1 cut(s) 492
TscAI CASTG 2 cut(s) 67, 97
TseFI GTSAC 1 cut(s) 199
TseI GCWGC 2 cut(s) 41, 353
Tsp45I GTSAC 1 cut(s) 199
TspDTI ATGAA 1 cut(s) 312
TspMI CCCGGG 1 cut(s) 265
TspRI CASTG 2 cut(s) 67, 97
XagI CCTNNNNNAGG 2 cut(s) 10, 491
XceI RCATGY 2 cut(s) 183, 225
XmaI CCCGGG 1 cut(s) 265
XmiI GTMKAC 1 cut(s) 261
XspI CTAG 4 cut(s) 257, 383, 519, 584
Zsp2I ATGCAT 1 cut(s) 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.