Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0003910

Physical Location & Seq

Genomic Coordinates Forward (+)

13592488 .. 13594839

Gene Structure 2352 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1174400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2352 bp

ATGATTACCCGCCAACTGAATTTCAAAGCCCTCCAACGAATACCTAACCAACGGTTCACGACATGCTCTAACTTCCACTCATTCAAACATGAGCACCAACTGTTCGACCAAAATCCCCAACCAAACGCAGCAGCATCGGTTAACCACGCTATGCTCAACAACTTACACAGAAACCTTCCGCGTCGAGCCCTCGGCATCTTCAGAGAGCAGGTTCAGTTATGTTCGTCACAGAATATCGATGAAGTTACTCTTACCCTTGCTGTCAAGGCTTGTCAAGGGGACCTCAAACCCGGGTGCCAAATTCACGGGTTTGCGGTTTCTTCTGGGTTTGCTTCTTATGTTACGGTTTTGAATTCCTTGATGAGTATGTACACGAAAGCTGGAGAGTTTGATAGTGCGTTATTTATCTTTGAGAGGATGTGTTATAAGGATATAGTTTCTTGGAATACAATTCTTTCGGGGTTCAGGTCGAGTGAGGGTGCATTGAGTTTTGCTATTAGGATGAACTTGAATGGAGTTGTTTTTGATCCAGTGACTTATACAACCGTTCTTTCCTTTTGTGCGGAGCATGAGGACTTTCTGTTTGGATGGCAGCTTCATTCTCTCATATTTAAGTCCGGTTTGGATTGTGAAGTTTTTGTTGGGAATGCACTCATAAGTATGTATTCGAGGCAGAGACATCTAATAGAAGCTAGGAACGTGTTTGATGAAATGTGGAATAAGGATCTGGTTTCATGGAATGCAATACTATCAGGTTATTCGCAAGAGGGAAATCACGGGCTTGAAGCGGTTTTGGCCTTTATTGAGATGATGAGAGAAGGGGTGGAGTTAGATCATGTCTCATTTACTAGTGCAGTTTCAGCTTGCGGTCATGAACAGAATTTAGGACTTGGGAAACAGATACATGGCGTGACTATAAAATCAGGATACGGAAGGCATGTTTCAGTTTGTAATGTATTGATCTCGACATATTCAAAGTGTGAGGATACTGAAGATGCAAAATTGGTGTTTCAGCACACGAACGATCGCAATGTAGTTTCTTGGACGACTATGATTTCTATGGACGAAGAAGATGCCATCTCTCTGTTTAATGAGATGAGACTTGATGGAGTATATCCAAATGATGTTACATTTGTTGGGCTAATCCACGCTGTAACGATTGGGAAGCAGGTGGAAGAAGGCCGAATGATTCACGGGTTTTGCATAAAGACTGGATTTTTGTCTAAGGATAATGTTTGCAACAGCTTTATCACAATGTATGCTAAGTTTGAGTCCATGCAGGACTCCATCAAAGTTTTCGAGGAGTTGGAATGCAGAGAAATCATATCGTGGAACGCTTTGATTTCAGGGTATGCTCAGAATGGGCTGTGCCAGGATGCTCTCAAAACATTCTTAGTAGCAACTATGGAGGCGACGCCAAACAATTACACATTTGGCAGTGTCTTGAGCGCAATTGGCGATGCTCACGATATATCTCTGAAGTATGGCCAACGATGCCACTCATTTTTAATCAAACTTGGATCAGTAACTGACCCGATCATCGCAGGTGCTCTTCTTGACATGTATGCAAAGCGAGGAAGTATTAGTGAGTCCGAAAGAGTTTTCAGTGAAACGCCTCACAAAAGTCAGTTTGCTTGGACTGCAATAATATCTGCATATGCTGCGCATGGAGACTGTGACTCGGTAATAGAATTGTTCGAGGAGATGGTAAGAGAAGGGGTTAAACCCGATTCAATCACGTTTCTTTCTGTACTAGCTGCCTGCAGCAGAAAGGGAATGGTTGAGACCGGGCGTCATCTCTTTCACTCAATGATCAAAGACTATCAGATTGAGACATCTCCTCAGCATTATTCAAGCATGGTGGACATGTTGGGGCGGGCAGGGAAACTGGAGGAAGCGGAGGAGTTGATCAGTCAGATGCCAGGACAGCCAGGGTTTTCGCTGCTGCAAAGCCTGCTCGGGGCATGCAGGATACACGGGAATGTGGAGATGGGTGAGAGAGTATCCAACGCGCTGATGGGATTGGAACCGACGGAGTCAGGTGCTTACGTGCAGATGTCGAACTTGTATGCTGAGAAAGGGGACTGGGAAATGGTGGCGAAAGTACGGAAATGGATGAGAGATAGGGGAGTGAGAAAGGAAGTTGGGTTTAGCTGGGTGGACACTGGTGGTGGTGATGCTGCTGATGGTTCCTTGTGTTTGCATCCGTTTTCGTCGGGAGATACGTCTCACCCGCTTTCGGAAGAGATTTGGAGAATGGCGAAATGCTTAGGATTGGAGATGAAAGTTCTGAGGGAGAATGTGAGGGAGACGAGATCACTGACTGTGAGGGCCTTGAATATGTACATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000960 GO:0000962 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010468 GO:0010604 GO:0016070 GO:0016787 GO:0016788 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0061013 GO:0061014 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363 GO:1903311 GO:1903313 GO:1905637 GO:1905639

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2

Protein Analysis

784

Amino Acids

87.45

Weight (kDa)

5.67

Isoelectric Point (pI)

35.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	242 - 289	1.2e-06	PPR repeat family
PPR_2	PF13041	344 - 384	7.1e-06	PPR repeat family
PPR_2	PF13041	542 - 589	7.3e-11	PPR repeat family
PPR	PF01535	545 - 574	2.2e-07	PPR repeat
E_motif	PF20431	659 - 721	1.7e-15	E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003910)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G32430 AT4G32430
fragaria_vesca	FvH4_1g03430
malus_domestica	MD15G1174400.v1.1
prunus_persica	Prupe.5G003100_v2.0.a1 Prupe.7G240400_v2.0.a1 Prupe.7G241500_v2.0.a1 Prupe.7G241700_v2.0.a1 Prupe.8G249000_v2.0.a1
pyrus_communis	pycom15g15600
rosa_chinensis	RchiOBHm_Chr2g0088661 RchiOBHm_Chr5g0002171
rosa_laevigata	RLG00000015973 RLG00000015974 RLG00000031004
rosa_multiflora	Rmu_sc0006835.1_g000012 Rmu_sc0006835.1_g000013 Rmu_sc0013155.1_g000001 Rmu_sc0015442.1_g000012 Rmu_sc0015442.1_g000013 Rmu_sc0024326.1_g000001 Rmu_sc0041985.1_g000001
rosa_roxburghii	Rroxscaffold_2G00152420
rosa_rugosa	Rorug01G0485700
rosa_samantha	Rh2AG041200 Rh2BG039900 Rh2CG040700 Rh2DG040300 Rh5AG016800 Rh5AG016900 Rh5CG018300 Rh5CG018400 Rh5CG018500
rosa_wichuraiana	Rw2G003320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	426
AarI	CACCTGC	2 cut(s)	1159, 1535
Acc16I	TGCGCA	1 cut(s)	1665
Acc36I	ACCTGC	3 cut(s)	199, 1159, 1535
AccB1I	GGYRCC	1 cut(s)	294
AccII	CGCG	2 cut(s)	181, 2012
AciI	CCGC	9 cut(s)	10, 179, 314, 563, 788, 867, 1876, 1898, 2234
AclWI	GGATC	3 cut(s)	521, 732, 1528
AcoI	YGGCCR	1 cut(s)	1486
AcsI	RAATTY	4 cut(s)	19, 300, 352, 880
AcuI	CTGAAG	3 cut(s)	184, 1011, 1499
AcyI	GRCGYC	2 cut(s)	1415, 1792
AfaI	GTAC	4 cut(s)	371, 1752, 2106, 2345
AfiI	CCNNNNNNNGG	2 cut(s)	1960, 2239
AflIII	ACRYGT	4 cut(s)	699, 1560, 1866, 2346
AgsI	TTSAA	9 cut(s)	25, 85, 352, 511, 785, 975, 1734, 1854, 2338
AhdI	GACNNNNNGTC	1 cut(s)	1791
AhlI	ACTAGT	1 cut(s)	848
AjnI	CCWGG	3 cut(s)	1371, 1921, 1930
AjuI	GAANNNNNNNTTGG	2 cut(s)	624, 656
AluBI	AGCT	7 cut(s)	380, 595, 692, 863, 1245, 1757, 2154
AluI	AGCT	7 cut(s)	380, 595, 692, 863, 1245, 1757, 2154
Alw21I	GWGCWC	2 cut(s)	96, 1552
Alw26I	GTCTC	8 cut(s)	670, 844, 1093, 1665, 1778, 1826, 2232, 2303
AlwI	GGATC	3 cut(s)	521, 732, 1528
AlwNI	CAGNNNCTG	1 cut(s)	1529
Ama87I	CYCGRG	2 cut(s)	290, 1958
AoxI	GGCC	4 cut(s)	795, 1180, 1486, 2331
ApeKI	GCWGC	9 cut(s)	128, 131, 592, 1661, 1757, 1764, 1942, 1945, 2180
ApoI	RAATTY	4 cut(s)	19, 300, 352, 880
ArsI	GACNNNNNNTTYG	4 cut(s)	566, 598, 1201, 1233
AspLEI	GCGC	3 cut(s)	1451, 1666, 2014
AspS9I	GGNCC	2 cut(s)	280, 2331
AsuC2I	CCSGG	3 cut(s)	291, 292, 1789
AsuHPI	GGTGA	3 cut(s)	2006, 2186, 2222
AvaI	CYCGRG	2 cut(s)	290, 1958
AvaII	GGWCC	1 cut(s)	280
BalI	TGGCCA	1 cut(s)	1488
BanI	GGYRCC	1 cut(s)	294
BanII	GRGCYC	1 cut(s)	190
Bbv12I	GWGCWC	2 cut(s)	96, 1552
BbvCI	CCTCAGC	1 cut(s)	1842
BbvI	GCAGC	9 cut(s)	140, 143, 604, 1648, 1744, 1776, 1929, 1932, 2167
BccI	CCATC	8 cut(s)	582, 1085, 1100, 1295, 1699, 1984, 2011, 2180
BcgI	CGANNNNNNTGC	4 cut(s)	117, 151, 1055, 1089
BciT130I	CCWGG	3 cut(s)	1373, 1923, 1932
BciVI	GTATCC	4 cut(s)	920, 979, 1965, 2014
BclI	TGATCA	2 cut(s)	1812, 1908
BcnI	CCSGG	3 cut(s)	291, 292, 1789
BcoDI	GTCTC	8 cut(s)	670, 844, 1093, 1665, 1778, 1826, 2232, 2303
BcuI	ACTAGT	1 cut(s)	848
BfaI	CTAG	3 cut(s)	693, 849, 1754
BfmI	CTRYAG	1 cut(s)	1762
BfuAI	ACCTGC	3 cut(s)	199, 1159, 1535
BfuI	GTATCC	4 cut(s)	920, 979, 1965, 2014
BisI	GCNGC	9 cut(s)	129, 132, 593, 1662, 1758, 1765, 1943, 1946, 2181
BlsI	GCNGC	9 cut(s)	130, 133, 594, 1663, 1759, 1766, 1944, 1947, 2182
Bme1390I	CCNGG	6 cut(s)	291, 292, 1373, 1789, 1923, 1932
Bme18I	GGWCC	1 cut(s)	280
BmeRI	GACNNNNNGTC	1 cut(s)	1791
BmeT110I	CYCGRG	2 cut(s)	290, 1958
BmgT120I	GGNCC	2 cut(s)	280, 2331
BmiI	GGNNCC	4 cut(s)	281, 296, 2028, 2191
BmrFI	CCNGG	6 cut(s)	291, 292, 1373, 1789, 1923, 1932
BmrI	ACTGGG	1 cut(s)	2095
BmuI	ACTGGG	1 cut(s)	2095
BpmI	CTGGAG	2 cut(s)	402, 1910
Bpu10I	CCTNAGC	2 cut(s)	1842, 2269
BpuEI	CTTGAG	1 cut(s)	1465
BpuMI	CCSGG	3 cut(s)	291, 292, 1789
Bsa29I	ATCGAT	1 cut(s)	237
BsaAI	YACGTR	1 cut(s)	2050
BsaHI	GRCGYC	2 cut(s)	1415, 1792
BsaI	GGTCTC	1 cut(s)	1778
BsaJI	CCNNGG	3 cut(s)	190, 290, 1931
BsaWI	WCCGGW	1 cut(s)	617
Bsc4I	CCNNNNNNNGG	2 cut(s)	1960, 2239
Bse1I	ACTGG	5 cut(s)	530, 1216, 1893, 2090, 2170
Bse3DI	GCAATG	1 cut(s)	1036
BseBI	CCWGG	3 cut(s)	1373, 1923, 1932
BseCI	ATCGAT	1 cut(s)	237
BseDI	CCNNGG	3 cut(s)	190, 290, 1931
BseGI	GGATG	6 cut(s)	423, 507, 593, 1381, 2121, 2203
BseLI	CCNNNNNNNGG	2 cut(s)	1960, 2239
BseMI	GCAATG	1 cut(s)	1036
BseMII	CTCAG	4 cut(s)	1370, 1856, 2064, 2282
BseNI	ACTGG	5 cut(s)	530, 1216, 1893, 2090, 2170
BseRI	GAGGAG	4 cut(s)	1316, 1715, 1830, 1916
BseXI	GCAGC	9 cut(s)	140, 143, 604, 1648, 1744, 1776, 1929, 1932, 2167
BseYI	CCCAGC	1 cut(s)	2154
BsgI	GTGCAG	2 cut(s)	873, 2072
Bsh1236I	CGCG	2 cut(s)	181, 2012
Bsh1285I	CGRYCG	1 cut(s)	1027
BshFI	GGCC	4 cut(s)	797, 1182, 1488, 2333
BshNI	GGYRCC	1 cut(s)	294
BshVI	ATCGAT	1 cut(s)	237
BsiEI	CGRYCG	1 cut(s)	1027
BsiHKAI	GWGCWC	2 cut(s)	96, 1552
BsiHKCI	CYCGRG	2 cut(s)	290, 1958
BsiSI	CCGG	3 cut(s)	291, 618, 1788
BslFI	GGGAC	2 cut(s)	293, 2096
BslI	CCNNNNNNNGG	2 cut(s)	1960, 2239
BsmAI	GTCTC	8 cut(s)	670, 844, 1093, 1665, 1778, 1826, 2232, 2303
BsmBI	CGTCTC	2 cut(s)	2232, 2303
BsmFI	GGGAC	2 cut(s)	293, 2096
BsmI	GAATGC	3 cut(s)	652, 745, 1316
BsnI	GGCC	4 cut(s)	797, 1182, 1488, 2333
Bso31I	GGTCTC	1 cut(s)	1778
BsoBI	CYCGRG	2 cut(s)	290, 1958
Bsp1286I	GDGCHC	3 cut(s)	96, 190, 1552
Bsp1407I	TGTACA	2 cut(s)	369, 2343
BspACI	CCGC	9 cut(s)	10, 179, 314, 563, 788, 867, 1876, 1898, 2234
BspANI	GGCC	4 cut(s)	797, 1182, 1488, 2333
BspCNI	CTCAG	4 cut(s)	1369, 1855, 2065, 2283
BspDI	ATCGAT	1 cut(s)	237
BspFNI	CGCG	2 cut(s)	181, 2012
BspHI	TCATGA	1 cut(s)	871
BspLI	GGNNCC	4 cut(s)	281, 296, 2028, 2191
BspMAI	CTGCAG	1 cut(s)	1766
BspMI	ACCTGC	3 cut(s)	199, 1159, 1535
BspPI	GGATC	3 cut(s)	521, 732, 1528
BspQI	GCTCTTC	1 cut(s)	1557
BspT107I	GGYRCC	1 cut(s)	294
BspTNI	GGTCTC	1 cut(s)	1778
BsrDI	GCAATG	1 cut(s)	1036
BsrGI	TGTACA	2 cut(s)	369, 2343
BsrI	ACTGG	5 cut(s)	530, 1216, 1893, 2090, 2170
BssECI	CCNNGG	3 cut(s)	190, 290, 1931
BssNI	GRCGYC	2 cut(s)	1415, 1792
Bst2UI	CCWGG	3 cut(s)	1373, 1923, 1932
Bst4CI	ACNGT	6 cut(s)	54, 102, 346, 547, 1676, 2326
Bst6I	CTCTTC	2 cut(s)	1557, 2238
BstACI	GRCGYC	2 cut(s)	1415, 1792
BstAPI	GCANNNNNTGC	2 cut(s)	1661, 1954
BstAUI	TGTACA	2 cut(s)	369, 2343
BstBAI	YACGTR	1 cut(s)	2050
BstC8I	GCNNGC	5 cut(s)	865, 1762, 1878, 1955, 1966
BstDEI	CTNAG	8 cut(s)	1224, 1263, 1356, 1393, 1842, 2073, 2269, 2291
BstF5I	GGATG	6 cut(s)	423, 507, 593, 1381, 2121, 2203
BstFNI	CGCG	2 cut(s)	181, 2012
BstHHI	GCGC	3 cut(s)	1451, 1666, 2014
BstMAI	GTCTC	8 cut(s)	670, 844, 1093, 1665, 1778, 1826, 2232, 2303
BstMCI	CGRYCG	1 cut(s)	1027
BstMWI	GCNNNNNNNGC	9 cut(s)	266, 794, 860, 1455, 1494, 1640, 1661, 1927, 1954
BstNI	CCWGG	3 cut(s)	1373, 1923, 1932
BstNSI	RCATGY	6 cut(s)	66, 941, 1564, 1870, 1968, 2350
BstSCI	CCNGG	6 cut(s)	289, 290, 1371, 1787, 1921, 1930
BstSFI	CTRYAG	1 cut(s)	1762
BstUI	CGCG	2 cut(s)	181, 2012
BstV1I	GCAGC	9 cut(s)	140, 143, 604, 1648, 1744, 1776, 1929, 1932, 2167
BstX2I	RGATCY	1 cut(s)	724
BstYI	RGATCY	1 cut(s)	724
Bsu15I	ATCGAT	1 cut(s)	237
BsuI	GTATCC	4 cut(s)	920, 979, 1965, 2014
BsuRI	GGCC	4 cut(s)	797, 1182, 1488, 2333
BsuTUI	ATCGAT	1 cut(s)	237
BtgZI	GCGATG	2 cut(s)	1473, 1525
BtsCI	GGATG	6 cut(s)	423, 507, 593, 1381, 2121, 2203
BtsI	GCAGTG	1 cut(s)	1444
BtsIMutI	CAGTG	5 cut(s)	537, 1444, 1612, 2163, 2318
BveI	ACCTGC	3 cut(s)	199, 1159, 1535
Cac8I	GCNNGC	5 cut(s)	865, 1762, 1878, 1955, 1966
CaiI	CAGNNNCTG	1 cut(s)	1529
CciI	TCATGA	1 cut(s)	871
CfoI	GCGC	3 cut(s)	1451, 1666, 2014
Cfr13I	GGNCC	2 cut(s)	280, 2331
Cfr9I	CCCGGG	1 cut(s)	290
ClaI	ATCGAT	1 cut(s)	237
CseI	GACGC	3 cut(s)	170, 1423, 1781
Csp6I	GTAC	4 cut(s)	370, 1751, 2105, 2344
CspCI	CAANNNNNGTGG	2 cut(s)	1842, 1877
CviQI	GTAC	4 cut(s)	370, 1751, 2105, 2344
DdeI	CTNAG	8 cut(s)	1224, 1263, 1356, 1393, 1842, 2073, 2269, 2291
DriI	GACNNNNNGTC	1 cut(s)	1791
EaeI	YGGCCR	1 cut(s)	1486
Eam1104I	CTCTTC	2 cut(s)	1557, 2238
Eam1105I	GACNNNNNGTC	1 cut(s)	1791
EarI	CTCTTC	2 cut(s)	1557, 2238
Eco24I	GRGCYC	1 cut(s)	190
Eco31I	GGTCTC	1 cut(s)	1778
Eco47I	GGWCC	1 cut(s)	280
Eco57I	CTGAAG	3 cut(s)	184, 1011, 1499
Eco88I	CYCGRG	2 cut(s)	290, 1958
EcoO109I	RGGNCCY	2 cut(s)	280, 2331
EcoRI	GAATTC	1 cut(s)	352
EcoRII	CCWGG	3 cut(s)	1371, 1921, 1930
EcoT38I	GRGCYC	1 cut(s)	190
Esp3I	CGTCTC	2 cut(s)	2232, 2303
FalI	AAGNNNNNCTT	2 cut(s)	234, 266
FaqI	GGGAC	2 cut(s)	293, 2096
FauI	CCCGC	3 cut(s)	17, 1869, 2241
FauNDI	CATATG	1 cut(s)	1657
FbaI	TGATCA	2 cut(s)	1812, 1908
Fnu4HI	GCNGC	9 cut(s)	129, 132, 593, 1662, 1758, 1765, 1943, 1946, 2181
FokI	GGATG	6 cut(s)	430, 514, 600, 1388, 2128, 2190
FriOI	GRGCYC	1 cut(s)	190
Fsp4HI	GCNGC	9 cut(s)	129, 132, 593, 1662, 1758, 1765, 1943, 1946, 2181
FspBI	CTAG	3 cut(s)	693, 849, 1754
FspI	TGCGCA	1 cut(s)	1665
GlaI	GCGC	3 cut(s)	1450, 1665, 2013
GluI	GCNGC	9 cut(s)	129, 132, 593, 1662, 1758, 1765, 1943, 1946, 2181
GsaI	CCCAGC	1 cut(s)	2158
GsuI	CTGGAG	2 cut(s)	402, 1910
HaeIII	GGCC	4 cut(s)	797, 1182, 1488, 2333
HapII	CCGG	3 cut(s)	291, 618, 1788
HgaI	GACGC	3 cut(s)	170, 1423, 1781
HhaI	GCGC	3 cut(s)	1451, 1666, 2014
Hin1I	GRCGYC	2 cut(s)	1415, 1792
Hin6I	GCGC	3 cut(s)	1449, 1664, 2012
HinP1I	GCGC	3 cut(s)	1449, 1664, 2012
HincII	GTYRAC	1 cut(s)	142
HindII	GTYRAC	1 cut(s)	142
HinfI	GANTC	7 cut(s)	1189, 1271, 1283, 1589, 1679, 1730, 2036
HpaI	GTTAAC	1 cut(s)	142
HpaII	CCGG	3 cut(s)	291, 618, 1788
HphI	GGTGA	3 cut(s)	2006, 2186, 2222
Hpy166II	GTNNAC	5 cut(s)	57, 142, 372, 1864, 2161
Hpy188I	TCNGA	8 cut(s)	203, 1359, 1479, 1594, 1827, 1917, 2242, 2292
Hpy188III	TCNNGA	8 cut(s)	58, 872, 924, 964, 1444, 1466, 1556, 2217
Hpy8I	GTNNAC	5 cut(s)	57, 142, 372, 1864, 2161
Hpy99I	CGWCG	4 cut(s)	186, 1417, 2035, 2218
HpyAV	CCTTC	5 cut(s)	185, 812, 927, 1172, 1709
HpyCH4III	ACNGT	6 cut(s)	54, 102, 346, 547, 1676, 2326
HpyCH4IV	ACGT	4 cut(s)	699, 1739, 2049, 2225
HpyF10VI	GCNNNNNNNGC	9 cut(s)	266, 794, 860, 1455, 1494, 1640, 1661, 1927, 1954
HpyF3I	CTNAG	8 cut(s)	1224, 1263, 1356, 1393, 1842, 2073, 2269, 2291
HpySE526I	ACGT	4 cut(s)	699, 1739, 2049, 2225
Hsp92I	GRCGYC	2 cut(s)	1415, 1792
HspAI	GCGC	3 cut(s)	1449, 1664, 2012
Ksp22I	TGATCA	2 cut(s)	1812, 1908
KspAI	GTTAAC	1 cut(s)	142
LguI	GCTCTTC	1 cut(s)	1557
LmnI	GCTCC	1 cut(s)	565
Lsp1109I	GCAGC	9 cut(s)	140, 143, 604, 1648, 1744, 1776, 1929, 1932, 2167
MaeI	CTAG	3 cut(s)	693, 849, 1754
MaeII	ACGT	4 cut(s)	699, 1739, 2049, 2225
MaeIII	GTNAC	9 cut(s)	225, 244, 340, 532, 910, 1126, 1153, 1525, 1676
MboII	GAAGA	8 cut(s)	190, 312, 1004, 1079, 1082, 1187, 1544, 2255
MfeI	CAATTG	1 cut(s)	1452
MflI	RGATCY	1 cut(s)	724
MhlI	GDGCHC	3 cut(s)	96, 190, 1552
MlsI	TGGCCA	1 cut(s)	1488
MluCI	AATT	9 cut(s)	19, 300, 352, 450, 880, 1001, 1423, 1452, 1691
MluNI	TGGCCA	1 cut(s)	1488
MlyI	GAGTC	5 cut(s)	1277, 1280, 1598, 1673, 2045
MmeI	TCCRAC	3 cut(s)	58, 1287, 2031
Mox20I	TGGCCA	1 cut(s)	1488
MscI	TGGCCA	1 cut(s)	1488
MseI	TTAA	5 cut(s)	141, 612, 1089, 1508, 1722
MslI	CAYNNNNRTG	2 cut(s)	659, 1980
Msp20I	TGGCCA	1 cut(s)	1488
MspI	CCGG	3 cut(s)	291, 618, 1788
MspR9I	CCNGG	6 cut(s)	291, 292, 1373, 1789, 1923, 1932
MunI	CAATTG	1 cut(s)	1452
Mva1269I	GAATGC	3 cut(s)	652, 745, 1316
MvaI	CCWGG	3 cut(s)	1373, 1923, 1932
MvnI	CGCG	2 cut(s)	181, 2012
MwoI	GCNNNNNNNGC	9 cut(s)	266, 794, 860, 1455, 1494, 1640, 1661, 1927, 1954
NciI	CCSGG	3 cut(s)	291, 292, 1789
NdeI	CATATG	1 cut(s)	1657
NlaIV	GGNNCC	4 cut(s)	281, 296, 2028, 2191
NmeAIII	GCCGAG	1 cut(s)	171
NmuCI	GTSAC	4 cut(s)	225, 532, 910, 1676
NsbI	TGCGCA	1 cut(s)	1665
NspI	RCATGY	6 cut(s)	66, 941, 1564, 1870, 1968, 2350
PaeI	GCATGC	1 cut(s)	1968
PagI	TCATGA	1 cut(s)	871
PaqCI	CACCTGC	2 cut(s)	1159, 1535
PciI	ACATGT	3 cut(s)	1560, 1866, 2346
PciSI	GCTCTTC	1 cut(s)	1557
PcsI	WCGNNNNNNNCGW	1 cut(s)	2222
PctI	GAATGC	3 cut(s)	652, 745, 1316
PfeI	GAWTC	2 cut(s)	1189, 1730
PflFI	GACNNNGTC	1 cut(s)	2035
PkrI	GCNGC	9 cut(s)	130, 133, 594, 1663, 1759, 1766, 1944, 1947, 2182
Ple19I	CGATCG	1 cut(s)	1027
PleI	GAGTC	5 cut(s)	1277, 1279, 1597, 1673, 2044
PpsI	GAGTC	5 cut(s)	1277, 1279, 1597, 1673, 2044
Ppu21I	YACGTR	1 cut(s)	2050
PpuMI	RGGWCCY	1 cut(s)	280
PscI	ACATGT	3 cut(s)	1560, 1866, 2346
PsiI	TTATAA	1 cut(s)	426
Psp5II	RGGWCCY	1 cut(s)	280
Psp6I	CCWGG	3 cut(s)	1371, 1921, 1930
PspFI	CCCAGC	1 cut(s)	2154
PspGI	CCWGG	3 cut(s)	1371, 1921, 1930
PspN4I	GGNNCC	4 cut(s)	281, 296, 2028, 2191
PspPI	GGNCC	2 cut(s)	280, 2331
PspPPI	RGGWCCY	1 cut(s)	280
PstI	CTGCAG	1 cut(s)	1766
PstNI	CAGNNNCTG	1 cut(s)	1529
PsuI	RGATCY	1 cut(s)	724
PsyI	GACNNNGTC	1 cut(s)	2035
PvuI	CGATCG	1 cut(s)	1027
RsaI	GTAC	4 cut(s)	371, 1752, 2106, 2345
RsaNI	GTAC	4 cut(s)	370, 1751, 2105, 2344
RseI	CAYNNNNRTG	2 cut(s)	659, 1980
SapI	GCTCTTC	1 cut(s)	1557
SaqAI	TTAA	5 cut(s)	141, 612, 1089, 1508, 1722
SatI	GCNGC	9 cut(s)	129, 132, 593, 1662, 1758, 1765, 1943, 1946, 2181
Sau96I	GGNCC	2 cut(s)	280, 2331
SchI	GAGTC	5 cut(s)	1277, 1280, 1598, 1673, 2045
ScrFI	CCNGG	6 cut(s)	291, 292, 1373, 1789, 1923, 1932
SduI	GDGCHC	3 cut(s)	96, 190, 1552
SfcI	CTRYAG	1 cut(s)	1762
SinI	GGWCC	1 cut(s)	280
SmaI	CCCGGG	1 cut(s)	292
SmiMI	CAYNNNNRTG	2 cut(s)	659, 1980
SmlI	CTYRAG	1 cut(s)	1444
SmoI	CTYRAG	1 cut(s)	1444
SpeI	ACTAGT	1 cut(s)	848
SphI	GCATGC	1 cut(s)	1968
Sse9I	AATT	9 cut(s)	19, 300, 352, 450, 880, 1001, 1423, 1452, 1691
SsiI	CCGC	9 cut(s)	10, 179, 314, 563, 788, 867, 1876, 1898, 2234
SspMI	CTAG	3 cut(s)	693, 849, 1754
StyD4I	CCNGG	6 cut(s)	289, 290, 1371, 1787, 1921, 1930
TaaI	ACNGT	6 cut(s)	54, 102, 346, 547, 1676, 2326
TaiI	ACGT	4 cut(s)	702, 1742, 2052, 2228
TaqI	TCGA	9 cut(s)	105, 184, 237, 470, 668, 965, 1299, 1698, 2060
TasI	AATT	9 cut(s)	19, 300, 352, 450, 880, 1001, 1423, 1452, 1691
TatI	WGTACW	3 cut(s)	369, 1750, 2343
TfiI	GAWTC	2 cut(s)	1189, 1730
Tru1I	TTAA	5 cut(s)	141, 612, 1089, 1508, 1722
Tru9I	TTAA	5 cut(s)	141, 612, 1089, 1508, 1722
TscAI	CASTG	5 cut(s)	537, 1444, 1612, 2170, 2325
TseFI	GTSAC	4 cut(s)	225, 532, 910, 1676
TseI	GCWGC	9 cut(s)	128, 131, 592, 1661, 1757, 1764, 1942, 1945, 2180
Tsp45I	GTSAC	4 cut(s)	225, 532, 910, 1676
TspDTI	ATGAA	7 cut(s)	255, 518, 587, 723, 723, 888, 2297
TspGWI	ACGGA	4 cut(s)	945, 2048, 2122, 2196
TspMI	CCCGGG	1 cut(s)	290
TspRI	CASTG	5 cut(s)	537, 1444, 1612, 2170, 2325
Tth111I	GACNNNGTC	1 cut(s)	2035
VpaK11BI	GGWCC	1 cut(s)	280
XapI	RAATTY	4 cut(s)	19, 300, 352, 880
XceI	RCATGY	6 cut(s)	66, 941, 1564, 1870, 1968, 2350
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2014
XmaI	CCCGGG	1 cut(s)	290
XspI	CTAG	3 cut(s)	693, 849, 1754

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.