Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0002237

Physical Location & Seq

Genomic Coordinates Reverse (-)

22057392 .. 22062677

Gene Structure 5286 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1259700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2280 bp

ATGGGTACCGATGCTGCTGAAGTGGCGGAGGCAGCGAGGACACCTCATTGCTTTAAGGTTCATCGTTTAATGTGCACGGAACTCCTGAGGTTGGTTGATAGAATCTCCAGGATACTTCCAGAAATAGAAGCAGCTCGACCTCGGTGCTCAACAGGAATACAGGCTCTATGCTTGTTAAATCGTGGAATGGAGAAGGCCAAGCTACTTCTTCAGCATTGCAGCGAGTCTAGTAAACTCTACCTGGCAGTTACCGGGGATGTGATGGTCTCAAGATGTCAAGGATCAAGGAACTTGTTGGAGCAAAGTGTACGAGAAATTCAGAACTGGGTTCCAGTGATGTTGGTGATGCAGCTGTCTCAAATAGCTGAGGATCTTAAAGGTTCAACATTTAAACTGGACCCGTATGAAGAAGAAGCTGGGAGGGTTGTCCGAGAATTGCTCCATGATGTTCCTTCTCAGTCAGACCCAAGGGAGGATTCTGAAATCAAGGCTTTTCAAATTGCATCCTTGAGGCTCCATATCACATCATCAAAGGCAATCTTAATTGAGAAAAGATCAATTAGGAAGTTGCTAGAAAAAGTTGGTGACAGTAACCCTCAGAAAAAGCAAATTTTGACTTATTTCATGTATCTATTGAAGAAGTATGGAAGCTTGATAACGGGAAAGCAAACAGAGAGTGCCTTAGAGCTGCAACAAGGAACGTTTGCTTCTGAGAATTCTGGAAATTGTTTTGAATCTAATCAATATGCTGAAGTTGAACCTCACGTTGGTTATGGCGAGTCCAAGGCACAAAGTAACTTGTTCCATGGAGCTGTACCTCCTGAGGAATTTATGTGCGGAATATCTTCCAAAATGATGTTCGATCCTGTTGTCATTGCTTCTGGTCAAACATATGAAAAAATGTGGATACAGAAGTGGTTTGATGAGGGTCATGATACATGCCCAAAAACTAATATGAAACTAACCAACCTGTCATTGACTCCGAACATTAACATGAAGGAGTTAATATCAAGGTGGTGTACACAGTATGGAGTTGTCCTTCCCGACCCAAGTATGCAACCAGAAGTCCTCTCATGGGAAACCTCTTCCACTTCCATTCGTAGTTTAGGCAGTTCTATGAATGATCTACACCTACAAATGGATTTCAGCTGTATATCGTTTGGGTCCTTAGATACCAGTTACAATTCTGATTCATCACGCAATAAGATTGAAAATGGCTTGAGTTTGGTGCAGAACGACAATGATTCTCTGAAATACCAGTATGCTAAAAAATGCGAGACAGGTGCTCAATTTCTATCTGAACTTGGTGAAGTCCAATGGGAATCCCAATGCAAGGCTGTTGAAGATGTCAAAAACCATTTGAAATGCAATCCTCAAGCTTCCTATGCTATGTCATCTAAGAATTTTGTTGAACCTCTCATCAAATTTTTGAGGGATGCACATGACCTGAATGATGCAAAAGCGCAGAGGGATGGGTTTAAATTATTACTGACCTTTGTGAGAAAAAACAGAAATGGAATACCTAATCTATGTGAAGAGGCATATAGTCTGCTGGCATGTTATCTTGATTCAAAGGTAACAGAAGAAGTTCTTGCCATAATGGAAGTTGTGTCTGGCCACCAGTATTGCAGGCCTAAGGTCTCAGAATCTGGTGCACTTACATCCATTCTAAAACTCCTGGACTCCCAGAGCAAAGATTTACAAGGACAAGCCATTAAGATTTTACGTAATTTGTCCTTAGATAAGGACATTTGCTCAAAAATTGTATCTTTGCAGTGCATACCAAAATTGGTTTCTTTCTTTAAAGATGACATTCTTTCAGGAGTCTGCATTTCTATATTGAAAAATTTGTGTGATACTGAAGAGGCCAGGATTTCGATTGCTGCAAGTAATGGATGCATTGCTTCTATTGCTGAACTCCTGGAGACAGGCAGCAGTGAGGACCAAGAACATGCTGTTGATATCCTCCTTTCTTTATGCTCTCAGCGTGATGAATATTGTCAGTTGGTCATGCACGAGGGTGTTATCCCATCGCTTGTCTATTTATCTAACAATGGGACAGAGAGAGCAATGACAAGCGCATTGGAAGTGCTACGGCTCTTAAGAGATATCAAATATGTCAATGAGCATGAGTGCTCTGGATCTGCCGACCTTGATGCATCCAGAGACCATGTAAATCATTCTACTGAAAGGAAATCACCAAAGATTTCAGGATTCTTTGGAAAGAAAATATCCATGTTCACAAAACCCAGTTCCCTTATACCGAAAAAGAAGAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

KAP U-box

Protein Analysis

760

Amino Acids

84.87

Weight (kDa)

6.09

Isoelectric Point (pI)

41.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
U-box	PF04564	274 - 342	6.6e-18	U-box domain
ARM_PUB	PF25598	470 - 709	2.1e-11	Plant U-box protein ARM repeat region
ARM_ARMC5	PF24768	529 - 701	3.7e-10	ARMC5
KAP	PF05804	549 - 650	1e-07	Kinesin-associated protein (KAP)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002237)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G36550
fragaria_vesca	FvH4_1g13090 FvH4_1g13090
malus_domestica	MD02G1145600.v1.1 MD15G1259700.v1.1
prunus_persica	Prupe.7G156200_v2.0.a1 Prupe.7G156200_v2.0.a1 Prupe.7G156200_v2.0.a1 Prupe.7G156200_v2.0.a1 Prupe.7G156200_v2.0.a1 Prupe.7G156200_v2.0.a1 Prupe.7G156200_v2.0.a1 Prupe.7G156200_v2.0.a1 Prupe.7G156200_v2.0.a1 Prupe.7G156200_v2.0.a1 Prupe.7G156200_v2.0.a1
pyrus_communis	pycom02g11520 pycom15g22690
rosa_chinensis	RchiOBHm_Chr2g0101271 RchiOBHm_Chr2g0104721 RchiOBHm_Chr2g0104731
rosa_laevigata	RLG00000017066 RLG00000017354
rosa_multiflora	Rmu_sc0006314.1_g000019
rosa_roxburghii	Rroxscaffold_2G00141450
rosa_rugosa	Rorug02G0099600 Rorug02G0122000 Rorug02G0122100 Rorug02G0122200
rosa_samantha	Rh2AG145600 Rh2AG172300 Rh2BG151000 Rh2BG179900 Rh2CG151400 Rh2CG177400 Rh2DG151100 Rh2DG178600
rosa_wichuraiana	Rw0G008640 Rw0G008650 Rw2G011360 Rw2G013490 Rw2G013550 Rw2G013560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	5
AccB1I	GGYRCC	1 cut(s)	5
AciI	CCGC	2 cut(s)	26, 837
AclI	AACGTT	1 cut(s)	701
AclWI	GGATC	4 cut(s)	289, 378, 857, 2149
AcoI	YGGCCR	1 cut(s)	1615
AcsI	RAATTY	7 cut(s)	315, 609, 715, 827, 1402, 1424, 1846
AcuI	CTGAAG	4 cut(s)	39, 194, 771, 1881
AfaI	GTAC	4 cut(s)	7, 309, 816, 1021
AfiI	CCNNNNNNNGG	6 cut(s)	91, 472, 767, 1075, 1138, 1333
AflII	CTTAAG	1 cut(s)	2101
AjnI	CCWGG	5 cut(s)	107, 240, 1677, 1868, 1920
AjuI	GAANNNNNNNTTGG	8 cut(s)	460, 492, 750, 782, 842, 874, 1777, 1809
AleI	CACNNNNGTG	1 cut(s)	2019
Alw21I	GWGCWC	5 cut(s)	77, 149, 1288, 1657, 2138
Alw26I	GTCTC	6 cut(s)	271, 360, 1271, 1645, 1919, 2160
Alw44I	GTGCAC	2 cut(s)	73, 1653
AlwI	GGATC	4 cut(s)	289, 378, 857, 2149
AlwNI	CAGNNNCTG	1 cut(s)	1649
AoxI	GGCC	4 cut(s)	195, 1615, 1631, 1866
ApaLI	GTGCAC	2 cut(s)	73, 1653
ApeKI	GCWGC	8 cut(s)	14, 32, 131, 219, 349, 688, 1883, 1932
ApoI	RAATTY	7 cut(s)	315, 609, 715, 827, 1402, 1424, 1846
Asp700I	GAANNNNTTC	2 cut(s)	844, 1587
Asp718I	GGTACC	1 cut(s)	5
AspLEI	GCGC	2 cut(s)	1465, 2081
AspS9I	GGNCC	3 cut(s)	397, 1164, 1942
AsuC2I	CCSGG	1 cut(s)	253
AsuHPI	GGTGA	4 cut(s)	355, 596, 1319, 2190
AvaII	GGWCC	3 cut(s)	397, 1164, 1942
AxyI	CCTNAGG	3 cut(s)	86, 822, 1635
BaeGI	GKGCMC	2 cut(s)	77, 1657
BaeI	ACNNNNGTAYC	2 cut(s)	899, 932
BalI	TGGCCA	1 cut(s)	1617
BanI	GGYRCC	1 cut(s)	5
BauI	CACGAG	1 cut(s)	2015
Bbv12I	GWGCWC	5 cut(s)	77, 149, 1288, 1657, 2138
BbvCI	CCTCAGC	1 cut(s)	366
BbvI	GCAGC	7 cut(s)	44, 143, 231, 361, 675, 1870, 1944
BccI	CCATC	3 cut(s)	256, 1466, 2038
BceAI	ACGGC	1 cut(s)	2111
BcgI	CGANNNNNNTGC	6 cut(s)	126, 160, 1265, 1299, 2138, 2172
BciT130I	CCWGG	5 cut(s)	109, 242, 1679, 1870, 1922
BciVI	GTATCC	2 cut(s)	105, 900
BcnI	CCSGG	1 cut(s)	253
BcoDI	GTCTC	6 cut(s)	271, 360, 1271, 1645, 1919, 2160
BfaI	CTAG	2 cut(s)	228, 572
BfrI	CTTAAG	1 cut(s)	2101
BfuI	GTATCC	2 cut(s)	105, 900
BisI	GCNGC	8 cut(s)	15, 33, 132, 220, 350, 689, 1884, 1933
BlsI	GCNGC	8 cut(s)	16, 34, 133, 221, 351, 690, 1885, 1934
Bme1390I	CCNGG	6 cut(s)	109, 242, 253, 1679, 1870, 1922
Bme18I	GGWCC	3 cut(s)	397, 1164, 1942
BmgT120I	GGNCC	3 cut(s)	397, 1164, 1942
BmiI	GGNNCC	5 cut(s)	7, 330, 399, 515, 1165
BmrFI	CCNGG	6 cut(s)	109, 242, 253, 1679, 1870, 1922
BmrI	ACTGGG	2 cut(s)	334, 2244
BmsI	GCATC	7 cut(s)	336, 512, 1426, 1444, 1886, 2146, 2168
BmuI	ACTGGG	2 cut(s)	334, 2244
BplI	GAGNNNNNCTC	4 cut(s)	28, 60, 423, 455
BpmI	CTGGAG	2 cut(s)	91, 1943
Bpu10I	CCTNAGC	1 cut(s)	366
BpuEI	CTTGAG	4 cut(s)	253, 529, 1240, 1359
BpuMI	CCSGG	1 cut(s)	253
BsaAI	YACGTR	1 cut(s)	1727
BsaI	GGTCTC	3 cut(s)	271, 1645, 2160
BsaJI	CCNNGG	5 cut(s)	140, 252, 467, 783, 805
Bsc4I	CCNNNNNNNGG	6 cut(s)	91, 472, 767, 1075, 1138, 1333
Bse1I	ACTGG	7 cut(s)	329, 332, 399, 1176, 1258, 1621, 2250
Bse21I	CCTNAGG	3 cut(s)	86, 822, 1635
Bse3DI	GCAATG	5 cut(s)	46, 214, 873, 1899, 2076
BseBI	CCWGG	5 cut(s)	109, 242, 1679, 1870, 1922
BseDI	CCNNGG	5 cut(s)	140, 252, 467, 783, 805
BseGI	GGATG	7 cut(s)	262, 503, 1441, 1477, 1661, 1901, 2159
BseLI	CCNNNNNNNGG	6 cut(s)	91, 472, 767, 1075, 1138, 1333
BseMI	GCAATG	5 cut(s)	46, 214, 873, 1899, 2076
BseMII	CTCAG	8 cut(s)	77, 357, 470, 611, 702, 813, 1656, 1997
BseNI	ACTGG	7 cut(s)	329, 332, 399, 1176, 1258, 1621, 2250
BseSI	GKGCMC	2 cut(s)	77, 1657
BseXI	GCAGC	7 cut(s)	44, 143, 231, 361, 675, 1870, 1944
BseYI	CCCAGC	1 cut(s)	416
BsgI	GTGCAG	1 cut(s)	1250
BshFI	GGCC	4 cut(s)	197, 1617, 1633, 1868
BshNI	GGYRCC	1 cut(s)	5
BsiHKAI	GWGCWC	5 cut(s)	77, 149, 1288, 1657, 2138
BsiSI	CCGG	1 cut(s)	252
BslFI	GGGAC	1 cut(s)	2071
BslI	CCNNNNNNNGG	6 cut(s)	91, 472, 767, 1075, 1138, 1333
BsmAI	GTCTC	6 cut(s)	271, 360, 1271, 1645, 1919, 2160
BsmFI	GGGAC	1 cut(s)	2071
BsnI	GGCC	4 cut(s)	197, 1617, 1633, 1868
Bso31I	GGTCTC	3 cut(s)	271, 1645, 2160
Bsp1286I	GDGCHC	5 cut(s)	77, 149, 1288, 1657, 2138
Bsp1407I	TGTACA	1 cut(s)	1019
Bsp143I	GATC	6 cut(s)	281, 370, 554, 862, 1123, 2141
Bsp19I	CCATGG	1 cut(s)	805
BspACI	CCGC	2 cut(s)	26, 837
BspANI	GGCC	4 cut(s)	197, 1617, 1633, 1868
BspCNI	CTCAG	8 cut(s)	78, 358, 469, 610, 703, 814, 1655, 1996
BspHI	TCATGA	1 cut(s)	931
BspLI	GGNNCC	5 cut(s)	7, 330, 399, 515, 1165
BspPI	GGATC	4 cut(s)	289, 378, 857, 2149
BspT107I	GGYRCC	1 cut(s)	5
BspTI	CTTAAG	1 cut(s)	2101
BspTNI	GGTCTC	3 cut(s)	271, 1645, 2160
BsrDI	GCAATG	5 cut(s)	46, 214, 873, 1899, 2076
BsrGI	TGTACA	1 cut(s)	1019
BsrI	ACTGG	7 cut(s)	329, 332, 399, 1176, 1258, 1621, 2250
BssECI	CCNNGG	5 cut(s)	140, 252, 467, 783, 805
BssMI	GATC	6 cut(s)	281, 370, 554, 862, 1123, 2141
BssSI	CACGAG	1 cut(s)	2015
BssT1I	CCWWGG	3 cut(s)	467, 783, 805
Bst2BI	CACGAG	1 cut(s)	2015
Bst2UI	CCWGG	5 cut(s)	109, 242, 1679, 1870, 1922
Bst4CI	ACNGT	2 cut(s)	590, 1026
Bst6I	CTCTTC	3 cut(s)	1090, 1530, 1857
BstAFI	CTTAAG	1 cut(s)	2101
BstAUI	TGTACA	1 cut(s)	1019
BstBAI	YACGTR	1 cut(s)	1727
BstC8I	GCNNGC	2 cut(s)	1554, 1631
BstDSI	CCRYGG	1 cut(s)	805
BstF5I	GGATG	7 cut(s)	262, 503, 1441, 1477, 1661, 1901, 2159
BstHHI	GCGC	2 cut(s)	1465, 2081
BstKTI	GATC	6 cut(s)	284, 373, 557, 865, 1126, 2144
BstMAI	GTCTC	6 cut(s)	271, 360, 1271, 1645, 1919, 2160
BstMBI	GATC	6 cut(s)	281, 370, 554, 862, 1123, 2141
BstMWI	GCNNNNNNNGC	4 cut(s)	23, 32, 1385, 1910
BstNI	CCWGG	5 cut(s)	109, 242, 1679, 1870, 1922
BstNSI	RCATGY	3 cut(s)	942, 1560, 1955
BstSCI	CCNGG	6 cut(s)	107, 240, 251, 1677, 1868, 1920
BstSLI	GKGCMC	2 cut(s)	77, 1657
BstSNI	TACGTA	1 cut(s)	1727
BstV1I	GCAGC	7 cut(s)	44, 143, 231, 361, 675, 1870, 1944
BstX2I	RGATCY	2 cut(s)	370, 2141
BstYI	RGATCY	2 cut(s)	370, 2141
Bsu36I	CCTNAGG	3 cut(s)	86, 822, 1635
BsuI	GTATCC	2 cut(s)	105, 900
BsuRI	GGCC	4 cut(s)	197, 1617, 1633, 1868
BtgI	CCRYGG	1 cut(s)	805
BtgZI	GCGATG	1 cut(s)	2016
BtsCI	GGATG	7 cut(s)	262, 503, 1441, 1477, 1661, 1901, 2159
BtsI	GCAGTG	2 cut(s)	1781, 1942
BtsIMutI	CAGTG	3 cut(s)	339, 1781, 1942
Cac8I	GCNNGC	2 cut(s)	1554, 1631
CaiI	CAGNNNCTG	1 cut(s)	1649
CciI	TCATGA	1 cut(s)	931
CfoI	GCGC	2 cut(s)	1465, 2081
Cfr13I	GGNCC	3 cut(s)	397, 1164, 1942
Csp6I	GTAC	4 cut(s)	6, 308, 815, 1020
CspCI	CAANNNNNGTGG	2 cut(s)	1607, 1642
CviQI	GTAC	4 cut(s)	6, 308, 815, 1020
DpnI	GATC	6 cut(s)	283, 372, 556, 864, 1125, 2143
DpnII	GATC	6 cut(s)	281, 370, 554, 862, 1123, 2141
DraI	TTTAAA	3 cut(s)	391, 1480, 1804
EaeI	YGGCCR	1 cut(s)	1615
Eam1104I	CTCTTC	3 cut(s)	1090, 1530, 1857
EarI	CTCTTC	3 cut(s)	1090, 1530, 1857
EciI	GGCGGA	1 cut(s)	41
Eco105I	TACGTA	1 cut(s)	1727
Eco130I	CCWWGG	3 cut(s)	467, 783, 805
Eco147I	AGGCCT	1 cut(s)	1633
Eco31I	GGTCTC	3 cut(s)	271, 1645, 2160
Eco32I	GATATC	2 cut(s)	1963, 2110
Eco47I	GGWCC	3 cut(s)	397, 1164, 1942
Eco57I	CTGAAG	4 cut(s)	39, 194, 771, 1881
Eco81I	CCTNAGG	3 cut(s)	86, 822, 1635
EcoO109I	RGGNCCY	1 cut(s)	1164
EcoRI	GAATTC	1 cut(s)	715
EcoRII	CCWGG	5 cut(s)	107, 240, 1677, 1868, 1920
EcoRV	GATATC	2 cut(s)	1963, 2110
EcoT14I	CCWWGG	3 cut(s)	467, 783, 805
EcoT22I	ATGCAT	2 cut(s)	1901, 2161
ErhI	CCWWGG	3 cut(s)	467, 783, 805
FalI	AAGNNNNNCTT	4 cut(s)	524, 556, 1575, 1607
FaqI	GGGAC	1 cut(s)	2071
FauNDI	CATATG	1 cut(s)	892
Fnu4HI	GCNGC	8 cut(s)	15, 33, 132, 220, 350, 689, 1884, 1933
FokI	GGATG	7 cut(s)	269, 490, 1448, 1484, 1648, 1908, 2146
Fsp4HI	GCNGC	8 cut(s)	15, 33, 132, 220, 350, 689, 1884, 1933
FspBI	CTAG	2 cut(s)	228, 572
GlaI	GCGC	2 cut(s)	1464, 2080
GluI	GCNGC	8 cut(s)	15, 33, 132, 220, 350, 689, 1884, 1933
GsaI	CCCAGC	1 cut(s)	420
GsuI	CTGGAG	2 cut(s)	91, 1943
HaeIII	GGCC	4 cut(s)	197, 1617, 1633, 1868
HapII	CCGG	1 cut(s)	252
HhaI	GCGC	2 cut(s)	1465, 2081
Hin6I	GCGC	2 cut(s)	1463, 2079
HinP1I	GCGC	2 cut(s)	1463, 2079
HindIII	AAGCTT	2 cut(s)	649, 1377
HpaII	CCGG	1 cut(s)	252
HphI	GGTGA	4 cut(s)	355, 596, 1319, 2190
Hpy166II	GTNNAC	7 cut(s)	75, 233, 308, 1020, 1022, 1655, 2241
Hpy8I	GTNNAC	7 cut(s)	75, 233, 308, 1020, 1022, 1655, 2241
HpyAV	CCTTC	4 cut(s)	187, 462, 991, 1049
HpyCH4III	ACNGT	2 cut(s)	590, 1026
HpyCH4IV	ACGT	3 cut(s)	701, 765, 1726
HpyF10VI	GCNNNNNNNGC	4 cut(s)	23, 32, 1385, 1910
HpySE526I	ACGT	3 cut(s)	701, 765, 1726
HspAI	GCGC	2 cut(s)	1463, 2079
KpnI	GGTACC	1 cut(s)	9
Kzo9I	GATC	6 cut(s)	281, 370, 554, 862, 1123, 2141
LmnI	GCTCC	4 cut(s)	298, 444, 519, 809
Lsp1109I	GCAGC	7 cut(s)	44, 143, 231, 361, 675, 1870, 1944
LweI	GCATC	7 cut(s)	336, 512, 1426, 1444, 1886, 2146, 2168
MaeI	CTAG	2 cut(s)	228, 572
MaeII	ACGT	3 cut(s)	701, 765, 1726
MaeIII	GTNAC	6 cut(s)	247, 584, 590, 794, 1178, 1576
MalI	GATC	6 cut(s)	283, 372, 556, 864, 1125, 2143
MboI	GATC	6 cut(s)	281, 370, 554, 862, 1123, 2141
MflI	RGATCY	2 cut(s)	370, 2141
MhlI	GDGCHC	5 cut(s)	77, 149, 1288, 1657, 2138
MlsI	TGGCCA	1 cut(s)	1617
MluNI	TGGCCA	1 cut(s)	1617
MlyI	GAGTC	5 cut(s)	233, 788, 973, 1676, 1833
MmeI	TCCRAC	1 cut(s)	276
Mox20I	TGGCCA	1 cut(s)	1617
Mph1103I	ATGCAT	2 cut(s)	1901, 2161
MroXI	GAANNNNTTC	2 cut(s)	844, 1587
MscI	TGGCCA	1 cut(s)	1617
MslI	CAYNNNNRTG	2 cut(s)	992, 2019
Msp20I	TGGCCA	1 cut(s)	1617
MspA1I	CMGCKG	2 cut(s)	352, 1149
MspCI	CTTAAG	1 cut(s)	2101
MspI	CCGG	1 cut(s)	252
MspR9I	CCNGG	6 cut(s)	109, 242, 253, 1679, 1870, 1922
MvaI	CCWGG	5 cut(s)	109, 242, 1679, 1870, 1922
MwoI	GCNNNNNNNGC	4 cut(s)	23, 32, 1385, 1910
NciI	CCSGG	1 cut(s)	253
NcoI	CCATGG	1 cut(s)	805
NdeI	CATATG	1 cut(s)	892
NdeII	GATC	6 cut(s)	281, 370, 554, 862, 1123, 2141
NlaIV	GGNNCC	5 cut(s)	7, 330, 399, 515, 1165
NmuCI	GTSAC	1 cut(s)	584
NsiI	ATGCAT	2 cut(s)	1901, 2161
NspI	RCATGY	3 cut(s)	942, 1560, 1955
OliI	CACNNNNGTG	1 cut(s)	2019
PagI	TCATGA	1 cut(s)	931
PceI	AGGCCT	1 cut(s)	1633
PdmI	GAANNNNTTC	2 cut(s)	844, 1587
PfeI	GAWTC	9 cut(s)	102, 476, 734, 1190, 1244, 1322, 1568, 1646, 2214
PfoI	TCCNGGA	3 cut(s)	107, 1677, 1920
PkrI	GCNGC	8 cut(s)	16, 34, 133, 221, 351, 690, 1885, 1934
PleI	GAGTC	5 cut(s)	232, 787, 973, 1676, 1832
PpsI	GAGTC	5 cut(s)	232, 787, 973, 1676, 1832
Ppu21I	YACGTR	1 cut(s)	1727
PpuMI	RGGWCCY	1 cut(s)	1164
Psp1406I	AACGTT	1 cut(s)	701
Psp5II	RGGWCCY	1 cut(s)	1164
Psp6I	CCWGG	5 cut(s)	107, 240, 1677, 1868, 1920
PspFI	CCCAGC	1 cut(s)	416
PspGI	CCWGG	5 cut(s)	107, 240, 1677, 1868, 1920
PspN4I	GGNNCC	5 cut(s)	7, 330, 399, 515, 1165
PspPI	GGNCC	3 cut(s)	397, 1164, 1942
PspPPI	RGGWCCY	1 cut(s)	1164
PstNI	CAGNNNCTG	1 cut(s)	1649
PsuI	RGATCY	2 cut(s)	370, 2141
PvuII	CAGCTG	2 cut(s)	352, 1149
RsaI	GTAC	4 cut(s)	7, 309, 816, 1021
RsaNI	GTAC	4 cut(s)	6, 308, 815, 1020
RseI	CAYNNNNRTG	2 cut(s)	992, 2019
SatI	GCNGC	8 cut(s)	15, 33, 132, 220, 350, 689, 1884, 1933
Sau3AI	GATC	6 cut(s)	281, 370, 554, 862, 1123, 2141
Sau96I	GGNCC	3 cut(s)	397, 1164, 1942
SchI	GAGTC	5 cut(s)	233, 788, 973, 1676, 1833
ScrFI	CCNGG	6 cut(s)	109, 242, 253, 1679, 1870, 1922
SduI	GDGCHC	5 cut(s)	77, 149, 1288, 1657, 2138
SfaNI	GCATC	7 cut(s)	336, 512, 1426, 1444, 1886, 2146, 2168
SinI	GGWCC	3 cut(s)	397, 1164, 1942
SmiMI	CAYNNNNRTG	2 cut(s)	992, 2019
SmlI	CTYRAG	5 cut(s)	268, 508, 1219, 1374, 2101
SmoI	CTYRAG	5 cut(s)	268, 508, 1219, 1374, 2101
SnaBI	TACGTA	1 cut(s)	1727
SseBI	AGGCCT	1 cut(s)	1633
SsiI	CCGC	2 cut(s)	26, 837
SspI	AATATT	1 cut(s)	1997
SspMI	CTAG	2 cut(s)	228, 572
StuI	AGGCCT	1 cut(s)	1633
StyD4I	CCNGG	6 cut(s)	107, 240, 251, 1677, 1868, 1920
StyI	CCWWGG	3 cut(s)	467, 783, 805
TaaI	ACNGT	2 cut(s)	590, 1026
TaiI	ACGT	3 cut(s)	704, 768, 1729
TaqI	TCGA	3 cut(s)	136, 861, 1877
TatI	WGTACW	1 cut(s)	1019
TfiI	GAWTC	9 cut(s)	102, 476, 734, 1190, 1244, 1322, 1568, 1646, 2214
TscAI	CASTG	3 cut(s)	339, 1781, 1942
TseFI	GTSAC	1 cut(s)	584
TseI	GCWGC	8 cut(s)	14, 32, 131, 219, 349, 688, 1883, 1932
Tsp45I	GTSAC	1 cut(s)	584
TspDTI	ATGAA	9 cut(s)	50, 420, 613, 909, 971, 1010, 1133, 1182, 2007
TspGWI	ACGGA	1 cut(s)	92
TspRI	CASTG	3 cut(s)	339, 1781, 1942
Vha464I	CTTAAG	1 cut(s)	2101
VneI	GTGCAC	2 cut(s)	73, 1653
VpaK11BI	GGWCC	3 cut(s)	397, 1164, 1942
XapI	RAATTY	7 cut(s)	315, 609, 715, 827, 1402, 1424, 1846
XceI	RCATGY	3 cut(s)	942, 1560, 1955
XmnI	GAANNNNTTC	2 cut(s)	844, 1587
XspI	CTAG	2 cut(s)	228, 572
Zsp2I	ATGCAT	2 cut(s)	1901, 2161

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.