Rh2DG151100

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0002237
Physical Location & Seq
Reverse (-)
12612352 .. 12617907
5556 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG151100.1

Sequence Viewer

Length: 1950 bp
ATGACGAAGTGTCACTTGCAGATCTCTAAAATAGTTGATGATCTTAAGGCTGCAACATTTAGACTGCATCCATCTGAAGAAGAGGCTGGGAAGGTCATCCGAGAATTGCTCCACCAAGATGCTTCTGCATCAAATTCAATAGAAGATTCTGAACTCAAGGCTCTTCAACTTGCATCTTTGAGGCTCCATATTACCACATCAAAGGAAGTCTTAATAGAGAAAAGATCTATTAAGAAGTTGCTACAAAAAGTTGGTGACAGTGACCCTCGAAAAAAGCAGATCTTGACATATTTCTTGTATCTATTGAAGAAGTATGGAAACTCAATATTGGTAAAGCAAACAGCGAGTGCCCCTGTGTGGCAACAAGGATCACTTGCTTCTGAGAATTCTGGCAACGACTTTGGATATAATCAGTGTGCTGAAGTTGAACCTCACACTGGTTATGGGGAATATGAGGCACAAAGTAACATGTTGCTTGGAGCTGTACCTCCTCAAGAATTTTTGTGCGGGATATCTTCCAGGTTGATGTACGATCCTGTTGTCATTGCTTCTGGACAAACATATGAAAGGATGTGGATACAGAAGTGGTTTAATGAGGGAAATGTTACATGCCCAAAAACTAATACCAAACTGGCCCACCTTTCATTGACTCCAAACGTTGGCATGAAGGAGTTGATATCAAGGTGGTGTACGGAGTCTGGAGTCACCATCCCCAACCCAAGTATGCAACCAGAAGCACTTGGCTCATGGGAAAACTCTTCCACTTCCATTGCTAGTTTAGGCAGTTCTATGAATGATCTACGTCTACAAATGGATCTCAGCACTATGTCATTTGGATCCTTAGATACCAGTTTCAATTCTAAGACAGAAGATGGCTCAAATCTGGTGCAGCTTGATGATGATTCTCTGAAATACCGATCTGCTAAAAAATGCGAGACAGATTTGGAATTTCTATCTAATATTAGTGAGCTCAAATGGGATTCCCAACGCAAGGCTGTTGAAGATGTTAAAAACGATTTGAAACGCAATCCTCAAGCTTCTTATGCTATGTCCTCTAAGAATTTTGTTGAACCTCTGATCAAATTTTTGAGGGATGCATATGATCAGGATGATGTAGAGGCTCAGAGAGATGGATTTAAATTATTATCTACCTTTGTCAGCAAAAGCAGAAACGGGATATGTTATTTAGGTGAAGACGTGTATAGTCTGTTAGTATCTTCTCTTGATTCAGAGGTAAAGGAAGAAGGTCTTGCCATAATGGAAATTCTGTCTGTCCACCAGGACTGCAGAGGTAACATCTCATCATCTGGTGCTCTTACATCCATTCTAAAACTTCTAGACTCTCAGAGCAGAAGTATACAAGGAAAAGCCCTTAAAATTTTGCACAATTTGTCCTCTGATAGAGATATTTGTTCAGAGATGGTATCTTTGGAGTGCATCCCAAAATTGGTTCCTTTCTTTGAAGACAGTGCTCTTACTGGAAACTGTATCGCTATATTGAAAAATCTAAGCGATTATGAAGGGGCCAGGATTTCTATTGCTGAAACTGACGGATGCATTGCTTATGTTGTTTATGTCCTAGACACTGGCAGTAAAGAGCATCAAGAGCATGCTGTTGATATCCTTCTTTCTTTATGCTCTCAGCGCATTGACTATTGTAACTTGGTCATGCATGAGGGTGTAATTCCTGCTCTTGTCCTCTCATCTACCAATGGGACAGAGAGAACAAGGATAAACGCAATGGAAGTTCTACGTCTCTTGAGAGATATTGATTATGACGATGAGCAACAATCTGCTGAACCTGAGCCTGAGGCAGCTAGAGATGAGCTACCTAGAGACAATGGAAATCACTCGAACGGGAAGAAAGCTTCTAAGGCATCAGGATTTTTCGGAAAGAAGTTATCAATATTCTCAAAACCGAGTTCTTTTTCACCGAAAAAGAAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KAP U-box

Protein Analysis

649

Amino Acids

72.08

Weight (kDa)

5.46

Isoelectric Point (pI)

43.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 164 - 232 6.2e-18 U-box domain
ARM_PUB PF25598 348 - 589 5.5e-14 Plant U-box protein ARM repeat region
KAP PF05804 376 - 534 1.2e-06 Kinesin-associated protein (KAP)
Arm_2 PF04826 400 - 500 8.2e-08 Armadillo-like
ARM_ARMC5 PF24768 423 - 583 1.1e-07 ARMC5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 659
AccI GTMKAC 2 cut(s) 805, 1357
AciI CCGC 1 cut(s) 507
AclI AACGTT 1 cut(s) 657
AclWI GGATC 5 cut(s) 376, 527, 822, 831, 844
AcsI RAATTY 8 cut(s) 133, 385, 497, 947, 1060, 1082, 1263, 1377
AcuI CTGAAG 2 cut(s) 96, 441
AfaI GTAC 3 cut(s) 486, 530, 691
AfiI CCNNNNNNNGG 5 cut(s) 357, 437, 659, 991, 1447
AflII CTTAAG 1 cut(s) 44
AflIII ACRYGT 2 cut(s) 468, 1197
AhdI GACNNNNNGTC 1 cut(s) 9
AjiI CACGTC 1 cut(s) 1198
AjnI CCWGG 3 cut(s) 518, 1278, 1526
AluBI AGCT 7 cut(s) 482, 892, 970, 1037, 1817, 1828, 1868
AluI AGCT 7 cut(s) 482, 892, 970, 1037, 1817, 1828, 1868
Alw21I GWGCWC 3 cut(s) 972, 1315, 1474
Alw26I GTCTC 3 cut(s) 929, 1760, 1830
AlwI GGATC 5 cut(s) 376, 527, 822, 831, 844
AoxI GGCC 2 cut(s) 633, 1524
ApeKI GCWGC 3 cut(s) 50, 889, 1814
ApoI RAATTY 8 cut(s) 133, 385, 497, 947, 1060, 1082, 1263, 1377
AspLEI GCGC 1 cut(s) 1647
AspS9I GGNCC 2 cut(s) 634, 1524
AsuHPI GGTGA 4 cut(s) 266, 697, 1202, 1923
AxyI CCTNAGG 1 cut(s) 1809
BaeGI GKGCMC 1 cut(s) 352
BaeI ACNNNNGTAYC 2 cut(s) 569, 602
BamHI GGATCC 1 cut(s) 836
BanII GRGCYC 1 cut(s) 972
BbsI GAAGAC 2 cut(s) 1200, 1470
Bbv12I GWGCWC 3 cut(s) 972, 1315, 1474
BbvI GCAGC 3 cut(s) 37, 901, 1826
BccI CCATC 5 cut(s) 79, 716, 866, 1124, 1414
BciT130I CCWGG 3 cut(s) 520, 1280, 1528
BciVI GTATCC 1 cut(s) 570
BclI TGATCA 2 cut(s) 1077, 1102
BcoDI GTCTC 3 cut(s) 929, 1760, 1830
BfaI CTAG 5 cut(s) 774, 1337, 1580, 1818, 1833
BfmI CTRYAG 1 cut(s) 1285
BfrI CTTAAG 1 cut(s) 44
BfuI GTATCC 1 cut(s) 570
BglII AGATCT 3 cut(s) 21, 224, 279
BisI GCNGC 3 cut(s) 51, 890, 1815
BlsI GCNGC 3 cut(s) 52, 891, 1816
Bme1390I CCNGG 3 cut(s) 520, 1280, 1528
BmeRI GACNNNNNGTC 1 cut(s) 9
BmgBI CACGTC 1 cut(s) 1198
BmgT120I GGNCC 2 cut(s) 634, 1524
BmiI GGNNCC 4 cut(s) 185, 838, 1452, 1525
BmrFI CCNGG 3 cut(s) 520, 1280, 1528
BmsI GCATC 9 cut(s) 76, 109, 137, 182, 1084, 1446, 1544, 1609, 1886
BpiI GAAGAC 2 cut(s) 1200, 1470
BplI GAGNNNNNCTC 2 cut(s) 93, 125
BpmI CTGGAG 1 cut(s) 720
Bpu10I CCTNAGC 1 cut(s) 1803
BpuEI CTTGAG 4 cut(s) 140, 477, 1017, 1780
Bsc4I CCNNNNNNNGG 5 cut(s) 357, 437, 659, 991, 1447
Bse1I ACTGG 5 cut(s) 442, 636, 849, 1483, 1591
Bse21I CCTNAGG 1 cut(s) 1809
Bse3DI GCAATG 4 cut(s) 543, 768, 1557, 1746
BseBI CCWGG 3 cut(s) 520, 1280, 1528
BseGI GGATG 9 cut(s) 67, 96, 576, 708, 1099, 1114, 1319, 1437, 1559
BseLI CCNNNNNNNGG 5 cut(s) 357, 437, 659, 991, 1447
BseMI GCAATG 4 cut(s) 543, 768, 1557, 1746
BseMII CTCAG 7 cut(s) 372, 832, 1136, 1358, 1655, 1794, 1800
BseNI ACTGG 5 cut(s) 442, 636, 849, 1483, 1591
BseRI GAGGAG 1 cut(s) 480
BseSI GKGCMC 1 cut(s) 352
BseXI GCAGC 3 cut(s) 37, 901, 1826
BseYI CCCAGC 1 cut(s) 86
BsgI GTGCAG 1 cut(s) 908
BshFI GGCC 2 cut(s) 635, 1526
BsiHKAI GWGCWC 3 cut(s) 972, 1315, 1474
BslFI GGGAC 1 cut(s) 1729
BslI CCNNNNNNNGG 5 cut(s) 357, 437, 659, 991, 1447
BsmAI GTCTC 3 cut(s) 929, 1760, 1830
BsmBI CGTCTC 1 cut(s) 1760
BsmFI GGGAC 1 cut(s) 1729
BsnI GGCC 2 cut(s) 635, 1526
Bsp1286I GDGCHC 4 cut(s) 352, 972, 1315, 1474
BspACI CCGC 1 cut(s) 507
BspANI GGCC 2 cut(s) 635, 1526
BspCNI CTCAG 7 cut(s) 373, 831, 1135, 1357, 1654, 1795, 1801
BspLI GGNNCC 4 cut(s) 185, 838, 1452, 1525
BspMAI CTGCAG 1 cut(s) 1289
BspPI GGATC 5 cut(s) 376, 527, 822, 831, 844
BspQI GCTCTTC 1 cut(s) 168
BspTI CTTAAG 1 cut(s) 44
BsrDI GCAATG 4 cut(s) 543, 768, 1557, 1746
BsrI ACTGG 5 cut(s) 442, 636, 849, 1483, 1591
BssNAI GTATAC 1 cut(s) 1358
Bst1107I GTATAC 1 cut(s) 1358
Bst2UI CCWGG 3 cut(s) 520, 1280, 1528
Bst4CI ACNGT 3 cut(s) 260, 1469, 1487
Bst6I CTCTTC 3 cut(s) 75, 168, 763
BstAFI CTTAAG 1 cut(s) 44
BstC8I GCNNGC 1 cut(s) 1611
BstF5I GGATG 9 cut(s) 67, 96, 576, 708, 1099, 1114, 1319, 1437, 1559
BstHHI GCGC 1 cut(s) 1647
BstMAI GTCTC 3 cut(s) 929, 1760, 1830
BstMWI GCNNNNNNNGC 4 cut(s) 1043, 1606, 1644, 1874
BstNI CCWGG 3 cut(s) 520, 1280, 1528
BstNSI RCATGY 3 cut(s) 472, 612, 1613
BstSCI CCNGG 3 cut(s) 518, 1278, 1526
BstSFI CTRYAG 1 cut(s) 1285
BstSLI GKGCMC 1 cut(s) 352
BstV1I GCAGC 3 cut(s) 37, 901, 1826
BstV2I GAAGAC 2 cut(s) 1200, 1470
BstX2I RGATCY 5 cut(s) 21, 224, 279, 814, 836
BstYI RGATCY 5 cut(s) 21, 224, 279, 814, 836
BstZ17I GTATAC 1 cut(s) 1358
Bsu36I CCTNAGG 1 cut(s) 1809
BsuI GTATCC 1 cut(s) 570
BsuRI GGCC 2 cut(s) 635, 1526
BtrI CACGTC 1 cut(s) 1198
BtsCI GGATG 9 cut(s) 67, 96, 576, 708, 1099, 1114, 1319, 1437, 1559
BtsIMutI CAGTG 5 cut(s) 265, 419, 435, 1474, 1584
Cac8I GCNNGC 1 cut(s) 1611
CfoI GCGC 1 cut(s) 1647
Cfr13I GGNCC 2 cut(s) 634, 1524
Csp6I GTAC 3 cut(s) 485, 529, 690
CspCI CAANNNNNGTGG 2 cut(s) 751, 786
CviAII CATG 7 cut(s) 469, 609, 664, 747, 1610, 1669, 1673
CviQI GTAC 3 cut(s) 485, 529, 690
DraI TTTAAA 1 cut(s) 1138
DriI GACNNNNNGTC 1 cut(s) 9
Eam1104I CTCTTC 3 cut(s) 75, 168, 763
Eam1105I GACNNNNNGTC 1 cut(s) 9
EarI CTCTTC 3 cut(s) 75, 168, 763
Ecl136II GAGCTC 1 cut(s) 970
Eco24I GRGCYC 1 cut(s) 972
Eco32I GATATC 3 cut(s) 513, 678, 1621
Eco53kI GAGCTC 1 cut(s) 970
Eco57I CTGAAG 2 cut(s) 96, 441
Eco81I CCTNAGG 1 cut(s) 1809
EcoICRI GAGCTC 1 cut(s) 970
EcoRI GAATTC 1 cut(s) 385
EcoRII CCWGG 3 cut(s) 518, 1278, 1526
EcoRV GATATC 3 cut(s) 513, 678, 1621
EcoT22I ATGCAT 3 cut(s) 1099, 1559, 1674
EcoT38I GRGCYC 1 cut(s) 972
Esp3I CGTCTC 1 cut(s) 1760
FaeI CATG 7 cut(s) 472, 612, 667, 750, 1613, 1672, 1676
FalI AAGNNNNNCTT 9 cut(s) 31, 194, 226, 266, 298, 357, 389, 1233, 1265
FaqI GGGAC 1 cut(s) 1729
FatI CATG 7 cut(s) 468, 608, 663, 746, 1609, 1668, 1672
FauI CCCGC 1 cut(s) 500
FauNDI CATATG 2 cut(s) 562, 1099
FbaI TGATCA 2 cut(s) 1077, 1102
FblI GTMKAC 2 cut(s) 805, 1357
Fnu4HI GCNGC 3 cut(s) 51, 890, 1815
FokI GGATG 9 cut(s) 54, 83, 583, 695, 1106, 1121, 1306, 1424, 1566
FriOI GRGCYC 1 cut(s) 972
Fsp4HI GCNGC 3 cut(s) 51, 890, 1815
FspBI CTAG 5 cut(s) 774, 1337, 1580, 1818, 1833
GlaI GCGC 1 cut(s) 1646
GluI GCNGC 3 cut(s) 51, 890, 1815
GsaI CCCAGC 1 cut(s) 90
GsuI CTGGAG 1 cut(s) 720
HaeIII GGCC 2 cut(s) 635, 1526
HhaI GCGC 1 cut(s) 1647
Hin1II CATG 7 cut(s) 472, 612, 667, 750, 1613, 1672, 1676
Hin6I GCGC 1 cut(s) 1645
HinP1I GCGC 1 cut(s) 1645
HindIII AAGCTT 2 cut(s) 1035, 1866
HinfI GANTC 8 cut(s) 146, 649, 695, 702, 902, 980, 1226, 1340
HphI GGTGA 4 cut(s) 266, 697, 1202, 1923
Hpy166II GTNNAC 4 cut(s) 690, 806, 1276, 1358
Hpy8I GTNNAC 4 cut(s) 690, 806, 1276, 1358
HpyAV CCTTC 5 cut(s) 85, 661, 1238, 1514, 1634
HpyCH4III ACNGT 3 cut(s) 260, 1469, 1487
HpyCH4IV ACGT 4 cut(s) 657, 802, 1197, 1753
HpyF10VI GCNNNNNNNGC 4 cut(s) 1043, 1606, 1644, 1874
HpySE526I ACGT 4 cut(s) 657, 802, 1197, 1753
Hsp92II CATG 7 cut(s) 472, 612, 667, 750, 1613, 1672, 1676
HspAI GCGC 1 cut(s) 1645
Ksp22I TGATCA 2 cut(s) 1077, 1102
LguI GCTCTTC 1 cut(s) 168
LmnI GCTCC 3 cut(s) 114, 189, 479
Lsp1109I GCAGC 3 cut(s) 37, 901, 1826
LweI GCATC 9 cut(s) 76, 109, 137, 182, 1084, 1446, 1544, 1609, 1886
MaeI CTAG 5 cut(s) 774, 1337, 1580, 1818, 1833
MaeII ACGT 4 cut(s) 657, 802, 1197, 1753
MaeIII GTNAC 8 cut(s) 11, 254, 260, 464, 604, 703, 1292, 1658
MflI RGATCY 5 cut(s) 21, 224, 279, 814, 836
MhlI GDGCHC 4 cut(s) 352, 972, 1315, 1474
MlyI GAGTC 4 cut(s) 643, 704, 711, 1334
Mph1103I ATGCAT 3 cut(s) 1099, 1559, 1674
MseI TTAA 7 cut(s) 45, 212, 231, 591, 1008, 1137, 1374
MslI CAYNNNNRTG 2 cut(s) 117, 1677
MspCI CTTAAG 1 cut(s) 44
MspR9I CCNGG 3 cut(s) 520, 1280, 1528
MvaI CCWGG 3 cut(s) 520, 1280, 1528
MwoI GCNNNNNNNGC 4 cut(s) 1043, 1606, 1644, 1874
NdeI CATATG 2 cut(s) 562, 1099
NlaIII CATG 7 cut(s) 472, 612, 667, 750, 1613, 1672, 1676
NlaIV GGNNCC 4 cut(s) 185, 838, 1452, 1525
NmuCI GTSAC 4 cut(s) 11, 254, 260, 703
NsiI ATGCAT 3 cut(s) 1099, 1559, 1674
NspI RCATGY 3 cut(s) 472, 612, 1613
PaeI GCATGC 1 cut(s) 1613
PciI ACATGT 1 cut(s) 468
PciSI GCTCTTC 1 cut(s) 168
PfeI GAWTC 4 cut(s) 146, 902, 980, 1226
PflMI CCANNNNNTGG 1 cut(s) 659
PkrI GCNGC 3 cut(s) 52, 891, 1816
PleI GAGTC 4 cut(s) 643, 703, 710, 1334
PpsI GAGTC 4 cut(s) 643, 703, 710, 1334
PscI ACATGT 1 cut(s) 468
Psp124BI GAGCTC 1 cut(s) 972
Psp1406I AACGTT 1 cut(s) 657
Psp6I CCWGG 3 cut(s) 518, 1278, 1526
PspFI CCCAGC 1 cut(s) 86
PspGI CCWGG 3 cut(s) 518, 1278, 1526
PspN4I GGNNCC 4 cut(s) 185, 838, 1452, 1525
PspPI GGNCC 2 cut(s) 634, 1524
PstI CTGCAG 1 cut(s) 1289
PsuI RGATCY 5 cut(s) 21, 224, 279, 814, 836
RsaI GTAC 3 cut(s) 486, 530, 691
RsaNI GTAC 3 cut(s) 485, 529, 690
RseI CAYNNNNRTG 2 cut(s) 117, 1677
SacI GAGCTC 1 cut(s) 972
SapI GCTCTTC 1 cut(s) 168
SaqAI TTAA 7 cut(s) 45, 212, 231, 591, 1008, 1137, 1374
SatI GCNGC 3 cut(s) 51, 890, 1815
Sau96I GGNCC 2 cut(s) 634, 1524
SchI GAGTC 4 cut(s) 643, 704, 711, 1334
ScrFI CCNGG 3 cut(s) 520, 1280, 1528
SduI GDGCHC 4 cut(s) 352, 972, 1315, 1474
SfaNI GCATC 9 cut(s) 76, 109, 137, 182, 1084, 1446, 1544, 1609, 1886
SfcI CTRYAG 1 cut(s) 1285
SmiI ATTTAAAT 1 cut(s) 1138
SmiMI CAYNNNNRTG 2 cut(s) 117, 1677
SmlI CTYRAG 5 cut(s) 44, 155, 492, 1032, 1759
SmoI CTYRAG 5 cut(s) 44, 155, 492, 1032, 1759
SphI GCATGC 1 cut(s) 1613
SsiI CCGC 1 cut(s) 507
SspI AATATT 3 cut(s) 327, 961, 1908
SspMI CTAG 5 cut(s) 774, 1337, 1580, 1818, 1833
SstI GAGCTC 1 cut(s) 972
StyD4I CCNGG 3 cut(s) 518, 1278, 1526
SwaI ATTTAAAT 1 cut(s) 1138
TaaI ACNGT 3 cut(s) 260, 1469, 1487
TaiI ACGT 4 cut(s) 660, 805, 1200, 1756
TaqI TCGA 2 cut(s) 268, 1853
TfiI GAWTC 4 cut(s) 146, 902, 980, 1226
Tru1I TTAA 7 cut(s) 45, 212, 231, 591, 1008, 1137, 1374
Tru9I TTAA 7 cut(s) 45, 212, 231, 591, 1008, 1137, 1374
TscAI CASTG 5 cut(s) 265, 419, 442, 1474, 1591
TseFI GTSAC 4 cut(s) 11, 254, 260, 703
TseI GCWGC 3 cut(s) 50, 889, 1814
Tsp45I GTSAC 4 cut(s) 11, 254, 260, 703
TspDTI ATGAA 5 cut(s) 579, 633, 680, 806, 1533
TspGWI ACGGA 2 cut(s) 707, 1566
TspRI CASTG 5 cut(s) 265, 419, 442, 1474, 1591
Van91I CCANNNNNTGG 1 cut(s) 659
Vha464I CTTAAG 1 cut(s) 44
XapI RAATTY 8 cut(s) 133, 385, 497, 947, 1060, 1082, 1263, 1377
XbaI TCTAGA 1 cut(s) 1336
XceI RCATGY 3 cut(s) 472, 612, 1613
XmiI GTMKAC 2 cut(s) 805, 1357
XspI CTAG 5 cut(s) 774, 1337, 1580, 1818, 1833
Zsp2I ATGCAT 3 cut(s) 1099, 1559, 1674
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.