Rh2AG172300

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0002237
Physical Location & Seq
Reverse (-)
16372989 .. 16379208
6220 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG172300.1

Sequence Viewer

Length: 3810 bp
ATGGCATTATCATTTGCACTGGATTTAATCGGAGGACAAGTATTTGAGCTGTTGTCTGCAAGTGTTACGCGAGCGGTGGACAGGAAAGCTGCGTTTGGAGACCTTCTCCAAGATATGAAATACACACTGAAGTCCTTGAAAAGACAGGCCATAGTGATTCAACAGATACAAGAGTATAATCTGGAACTGAGTCTCCCAAATGATGAAATGATTGCTCTTGTAACACAAATGACAGTTGGAAGACAACTTGTTGATAAGTCGTCAGGGATTAGATTTCGTTTGTGCAACTTCTGCTGCATAAATGATTTGGCTGACCAACTTAAAGAATTGAATAGTTCTCTTGAAAGTCTGTTTGACAACCTGCAGTTGGAGCAAGTAAGGGATATTAAGGAGACCTTGAAATTGTCAAGACAAAACCGCGATGATCTTGAGAAAATGAAGGTCATGTTGGAGAAGATAATGCAGATGATTGATGATCCTGAAGTGAAGGGTCTACTGAAGAAGTTAGTGGAGCAGAATGCAGGCGGATCAATGAAGAGCTCGCCCTCAACCTCAACCTTGGGCTCAGGAAGTTGCACAGAAAATGAAGGGAATTGCGAAACACAAGTGCCGTCATCAAAAGCACTAGTAGAAGCAGCATTTCGGGTGCTACTTAATGCCGTTATAGAAGTGAAGGAGAGCACTATTATGTTTAGACCTCTCCTCCGACGTCTCAAATCTACACTAGACTATTTACTGCCATTGATTGGAGATATGGTACAATGTAACAAGGCATTAGATCGCCCAAAAGAGGAACTAGAAAATATGAGAATACAAATGGAGAATGGTGTAGTGCTTGTTGGCAAGTGCTTAAATGTTCGTCGGTGGACAAGAAACAAAAAGTATATATACACCAACCAACTTTTTGAATTGAATGATTATCTTCAAGAGCAGTTAAGTATACTGCTAGTGGAGATAGCAAGGGATGCGAGGGAGACCTCGGTGGTGATAAGGAATATTAAGGACATGGTCAAGAGAGATGAAAGGAGTGGTATGGCAGAAAATCAACTTGAATATAAAGGTTTTTGTAATGTATCTGAACCTCCATTGCCTACTGTTGGACTCGAAACAAACATGCAAAGAACAAGGGATGTGATGATGGAGACATTGGTTTCTGTAAAGAATATCGAGGAGGGGGTCAAGGGAATGGAAGGGAGAATTATAGATGGAGTATCTGAAACTCTGTCGCCTACTGTTGGAAGTGAAGTACTCAACATTCAGGGAACAAAGGATGTGATCAAGGAGACACAGGCTTCAACAGAGAATACAAATCCAGAGGTTAACCTAATTGAAGGCAGAGCTGAGTCCCAGTTTGGCTCCGTTGCATCTTCAGAGAGCCAGCAGCAAACAGAGGGAATCAGTAAAGTACATGGAACTTTAGGTCACTGTGAACTGGAAAATTCAGCATCTCAAGAGACCTTTTCACTATACCTTCCTGATTCCATCCCTCTAGAGTTCAATGCATTGAAGAAGGAAAATGTATTTCCTGACAGACCTGGCCAGCCTGACTGTCTTGACAAGGAGTACAAAATCAACGGGACAGTGAGAAAGCCTATTTCCTTTAAGGTTCACCGTTCAATGTGCATTGAACTTTGGAAGTTGGTGGAGAGAATTTCAAAGATATTTCCCGACATAGAAGCAGCAAGACCTCGTTACTCAACGGGAATACAGGCTCTATGCATGTTGAATCGTGCAATTGAGAAAGCCAAGCAACTTGTTCAGTATTGCAGCGAGTCTAGCAAACTCTACCTGGCGTTTACAGGGGATGTGATAGTCTCAAGATGTCAAAAATCAAGGAACTTGTTGGAGCAAAGTTTGTGCCAGATTCAGAGTTGGGTTCCAGTGATTTTGGTGGCAGAGATCTCTAAAATAGTTGATGATCTTAAGGCTGCAACATTTAGACTGCATCCATCTGAAGAAGAGGCTGGGAAGGTCATCCGAGAATTGCTCCACCAAGATGCTTCTGCATCAAATTCAATAGAAGATTCTGAACTCAAGGCTCTTCAACTTGCATCTTTGAGGCTCCATATTACCACATCAGAGGAAGTCTTAATAGAGAAAAGATCTATTAAGAAGTTGCTACAAAAAGTTGGTGACAGTGACCCTCGAAAAAAGCAGATCTTGACATATTTCTTGTATCTAATGAAGAAGTATGGAAACTTAATATTGGTAAAGCAAACAGAGAGTGCCCCTGTGTGGCAACAAGGATCACTTGCTTCTGAGAATTCTGGCAACGACTTTGGATATAATCAGTGTGCTGAAGTTGAACCTCACACTGGTTATGGGGAATATGAGGCACAAAGTAACATGTTGCTTGGAGCTGTACCTCCTCAAGAATTTTTGTGCGGGATATCTTCCAGGTTGATGTACGATCCTGTTGTCATTGCTTCTGGACAAACATATGAAAGGATGTGGATACAGAAGTGGTTTAATGAGGGAAATGATACATGCCCAAAAACTAATACCAAACTGGCCCACCTTTCATTGAATCCAAACGTTGGCATGAAGGAGTTGATATCAAGGTGGTGTACAAAGTCTGGAGTCACCATCCCCAACCCAAGTATGCAACCAGAAGCACTTGGCTCATGGGAAAACTCTTCCACTTCCATTGCTAGTTTAGGCAGTTCTATGAATGATCTACGTCTACAAATGGATCTCAGCACCATGTCATTTGGATCCTTAGATACCAGTTTCAATTCTAAGACAGAAGATGGCTCAAATCTGGTGCAGATTGATGATGATTCTCTGAAATACCAATCTGCTAAAAAATGCGAGACAGATTTGGAATTTCTATCTAATATTAGTGAGCTCAAATGGGATTCCCAACGCAAGGCTGTTGAAGATGTTAAAAACGATTTGAAACGCAATCCTCAAGCTTCTTATGCTATGTCCTCTAAGAATTTTGTTGAACCTCTCATCAAATTTTTGAGGGATGCATATGATCAGGATGATGTAGAGGCTCAGAGAGATGGATTTAAATTATTATCTACCTTTGTCAGCAAAAACAGAAACGGGATATGTTATTTAGGTGAAGACGTGTATAGTCTGTTAGTATCTTCTCTTGATTCAGAGGTAAAGGAAGAAGGTCTTGCCATAATGGAAATTCTGTCTGTCCACCAGGACTGCAGAGGTAACATCTCATCATCTGGTGCTCTTACATCCATTCTAAAACTTCTAGACTCTCAGAGCAGAAGTATACAAGGAAAAGCCCTTAAAATTTTGCACAATTTGTCCTTCGATAGAGATATTTGTTCAGAGATGGTATCTTTGGAGTGCATCCCAAAATTGGTTCCTTTCTTTGAAGACAGTGCTCTTACTGGAAACTGTATCGCTATATTGAAAAATCTAAGCGATTATGAAGGGGCCAGGATTTCTATTGCTGAAACTGATGGATGCATTGCTTATGCTGTTTATGTCCTAGACACTGGCAGTAAAGAGCATCAAGAGCATGCTGTTGATATCCTTCTTTCTTTATGCTCTCAGCGCATTGACTATTGTAACTTGGTCATGCATGAGGGTGTAATTCCTGCTCTTGTCCTCTCATCTACCAATGGGACAGAGAGAACAAGGATAAACGCAATGGAAGTTCTACGTCTCTTGAGAGATATTGATTATGACGATGAGCAAGAATCTGCTGAACCTGAGCCTGAGGCAGCTAGAGATGAGCTACCTAGAGACAATGGAAATCACTGGAACGGGAAGAAAGCTTCTAAGGCATCAGGATTTTTCGGAAAGAAGTTATCAATGTTCTCAAAACCGAGTTCTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KAP U-box

Protein Analysis

1269

Amino Acids

142.07

Weight (kDa)

5.22

Isoelectric Point (pI)

41.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 12 - 143 9.1e-13 Arabidopsis broad-spectrum mildew resistance protein RPW8
RPW8 PF05659 208 - 332 1.7e-23 Arabidopsis broad-spectrum mildew resistance protein RPW8
U-box PF04564 790 - 858 2.5e-17 U-box domain
ARM_PUB PF25598 976 - 1215 3.2e-12 Plant U-box protein ARM repeat region
KAP PF05804 1005 - 1160 2.7e-07 Kinesin-associated protein (KAP)
Arm_2 PF04826 1026 - 1126 9.7e-07 Armadillo-like
ARM_ARMC5 PF24768 1049 - 1210 2.8e-06 ARMC5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 712
Acc36I ACCTGC 1 cut(s) 369
AccB7I CCANNNNNTGG 2 cut(s) 746, 2537
AccBSI CCGCTC 1 cut(s) 74
AccI GTMKAC 4 cut(s) 493, 940, 2683, 3235
AccII CGCG 2 cut(s) 70, 420
AciI CCGC 4 cut(s) 74, 418, 525, 2385
AclI AACGTT 1 cut(s) 2535
AclWI GGATC 7 cut(s) 470, 535, 2254, 2405, 2700, 2709, 2722
AcoI YGGCCR 1 cut(s) 1537
AcuI CTGAAG 6 cut(s) 149, 501, 518, 1353, 1974, 2319
AcyI GRCGYC 1 cut(s) 709
AfaI GTAC 7 cut(s) 759, 1248, 1407, 1565, 2364, 2408, 2569
AflII CTTAAG 1 cut(s) 1922
AflIII ACRYGT 2 cut(s) 2346, 3075
AhlI ACTAGT 1 cut(s) 625
AjiI CACGTC 1 cut(s) 3076
AjnI CCWGG 5 cut(s) 1534, 1788, 2396, 3156, 3404
AjuI GAANNNNNNNTTGG 2 cut(s) 431, 463
AloI GAACNNNNNNTCC 2 cut(s) 177, 209
Alw21I GWGCWC 5 cut(s) 542, 683, 2850, 3193, 3352
AlwI GGATC 7 cut(s) 470, 535, 2254, 2405, 2700, 2709, 2722
AoxI GGCC 4 cut(s) 147, 1537, 2511, 3402
ApeKI GCWGC 8 cut(s) 89, 294, 635, 1381, 1679, 1767, 1928, 3692
AspLEI GCGC 1 cut(s) 3525
AspS9I GGNCC 2 cut(s) 2512, 3402
AsuHPI GGTGA 5 cut(s) 997, 1601, 2144, 2575, 3080
AxyI CCTNAGG 1 cut(s) 3687
BaeGI GKGCMC 1 cut(s) 2230
BaeI ACNNNNGTAYC 4 cut(s) 158, 191, 2447, 2480
BalI TGGCCA 1 cut(s) 1539
BamHI GGATCC 1 cut(s) 2714
BanII GRGCYC 3 cut(s) 542, 566, 2850
BbsI GAAGAC 3 cut(s) 247, 3078, 3348
Bbv12I GWGCWC 5 cut(s) 542, 683, 2850, 3193, 3352
BbvI GCAGC 8 cut(s) 76, 281, 647, 1393, 1691, 1779, 1915, 3704
BccI CCATC 9 cut(s) 1132, 1199, 1490, 1957, 2594, 2744, 3002, 3292, 3422
BceAI ACGGC 2 cut(s) 595, 644
BciT130I CCWGG 5 cut(s) 1536, 1790, 2398, 3158, 3406
BciVI GTATCC 1 cut(s) 2448
BclI TGATCA 2 cut(s) 1275, 2980
BcuI ACTAGT 1 cut(s) 625
BfmI CTRYAG 2 cut(s) 362, 3163
BfrI CTTAAG 1 cut(s) 1922
BfuAI ACCTGC 1 cut(s) 369
BfuI GTATCC 1 cut(s) 2448
BglII AGATCT 3 cut(s) 1899, 2102, 2157
BisI GCNGC 8 cut(s) 90, 295, 636, 1382, 1680, 1768, 1929, 3693
BlsI GCNGC 8 cut(s) 91, 296, 637, 1383, 1681, 1769, 1930, 3694
BmcAI AGTACT 1 cut(s) 1248
Bme1390I CCNGG 5 cut(s) 1536, 1790, 2398, 3158, 3406
BmgBI CACGTC 1 cut(s) 3076
BmgT120I GGNCC 2 cut(s) 2512, 3402
BmiI GGNNCC 6 cut(s) 1357, 1878, 2063, 2716, 3330, 3403
BmrFI CCNGG 5 cut(s) 1536, 1790, 2398, 3158, 3406
BmrI ACTGGG 1 cut(s) 1342
BmuI ACTGGG 1 cut(s) 1342
BpiI GAAGAC 3 cut(s) 247, 3078, 3348
BplI GAGNNNNNCTC 4 cut(s) 90, 122, 1971, 2003
BpmI CTGGAG 1 cut(s) 2598
Bpu10I CCTNAGC 2 cut(s) 565, 3681
BpuEI CTTGAG 7 cut(s) 449, 1434, 1801, 2018, 2355, 2895, 3658
BsaHI GRCGYC 1 cut(s) 709
BsaI GGTCTC 4 cut(s) 93, 386, 968, 1448
BsaJI CCNNGG 2 cut(s) 558, 978
BsaXI ACNNNNNCTCC 8 cut(s) 24, 54, 177, 207, 687, 717, 741, 771
Bse21I CCTNAGG 1 cut(s) 3687
Bse3DI GCAATG 5 cut(s) 1085, 2421, 2646, 3435, 3624
BseBI CCWGG 5 cut(s) 1536, 1790, 2398, 3158, 3406
BseDI CCNNGG 2 cut(s) 558, 978
BseMI GCAATG 5 cut(s) 1085, 2421, 2646, 3435, 3624
BseRI GAGGAG 3 cut(s) 692, 1184, 2358
BseSI GKGCMC 1 cut(s) 2230
BseXI GCAGC 8 cut(s) 76, 281, 647, 1393, 1691, 1779, 1915, 3704
BseYI CCCAGC 1 cut(s) 1964
BsgI GTGCAG 1 cut(s) 2786
Bsh1236I CGCG 2 cut(s) 70, 420
BshFI GGCC 4 cut(s) 149, 1539, 2513, 3404
BsiHKAI GWGCWC 5 cut(s) 542, 683, 2850, 3193, 3352
BslFI GGGAC 3 cut(s) 1330, 1591, 3607
BsmBI CGTCTC 2 cut(s) 716, 3638
BsmFI GGGAC 3 cut(s) 1330, 1591, 3607
BsmI GAATGC 1 cut(s) 523
BsnI GGCC 4 cut(s) 149, 1539, 2513, 3404
Bso31I GGTCTC 4 cut(s) 93, 386, 968, 1448
Bsp1286I GDGCHC 7 cut(s) 542, 566, 683, 2230, 2850, 3193, 3352
Bsp1407I TGTACA 1 cut(s) 2567
BspACI CCGC 4 cut(s) 74, 418, 525, 2385
BspANI GGCC 4 cut(s) 149, 1539, 2513, 3404
BspFNI CGCG 2 cut(s) 70, 420
BspLI GGNNCC 6 cut(s) 1357, 1878, 2063, 2716, 3330, 3403
BspMAI CTGCAG 2 cut(s) 366, 3167
BspMI ACCTGC 1 cut(s) 369
BspPI GGATC 7 cut(s) 470, 535, 2254, 2405, 2700, 2709, 2722
BspQI GCTCTTC 2 cut(s) 530, 2046
BspTI CTTAAG 1 cut(s) 1922
BspTNI GGTCTC 4 cut(s) 93, 386, 968, 1448
BsrBI CCGCTC 1 cut(s) 74
BsrDI GCAATG 5 cut(s) 1085, 2421, 2646, 3435, 3624
BsrGI TGTACA 1 cut(s) 2567
BssECI CCNNGG 2 cut(s) 558, 978
BssNAI GTATAC 2 cut(s) 941, 3236
BssNI GRCGYC 1 cut(s) 709
BssT1I CCWWGG 1 cut(s) 558
Bst1107I GTATAC 2 cut(s) 941, 3236
Bst2UI CCWGG 5 cut(s) 1536, 1790, 2398, 3158, 3406
Bst6I CTCTTC 4 cut(s) 530, 1953, 2046, 2641
BstACI GRCGYC 1 cut(s) 709
BstAFI CTTAAG 1 cut(s) 1922
BstAPI GCANNNNNTGC 2 cut(s) 291, 965
BstAUI TGTACA 1 cut(s) 2567
BstC8I GCNNGC 6 cut(s) 72, 523, 542, 1379, 1541, 3489
BstFNI CGCG 2 cut(s) 70, 420
BstHHI GCGC 1 cut(s) 3525
BstMWI GCNNNNNNNGC 8 cut(s) 291, 370, 965, 1776, 2921, 3484, 3522, 3752
BstNI CCWGG 5 cut(s) 1536, 1790, 2398, 3158, 3406
BstNSI RCATGY 5 cut(s) 1117, 1723, 2350, 2490, 3491
BstSCI CCNGG 5 cut(s) 1534, 1788, 2396, 3156, 3404
BstSFI CTRYAG 2 cut(s) 362, 3163
BstSLI GKGCMC 1 cut(s) 2230
BstUI CGCG 2 cut(s) 70, 420
BstV1I GCAGC 8 cut(s) 76, 281, 647, 1393, 1691, 1779, 1915, 3704
BstV2I GAAGAC 3 cut(s) 247, 3078, 3348
BstX2I RGATCY 5 cut(s) 1899, 2102, 2157, 2692, 2714
BstYI RGATCY 5 cut(s) 1899, 2102, 2157, 2692, 2714
BstZ17I GTATAC 2 cut(s) 941, 3236
Bsu36I CCTNAGG 1 cut(s) 3687
BsuI GTATCC 1 cut(s) 2448
BsuRI GGCC 4 cut(s) 149, 1539, 2513, 3404
BtgZI GCGATG 1 cut(s) 435
BtrI CACGTC 1 cut(s) 3076
BveI ACCTGC 1 cut(s) 369
Cac8I GCNNGC 6 cut(s) 72, 523, 542, 1379, 1541, 3489
CfoI GCGC 1 cut(s) 3525
Cfr13I GGNCC 2 cut(s) 2512, 3402
Csp6I GTAC 7 cut(s) 758, 1247, 1406, 1564, 2363, 2407, 2568
CspCI CAANNNNNGTGG 2 cut(s) 2629, 2664
CviQI GTAC 7 cut(s) 758, 1247, 1406, 1564, 2363, 2407, 2568
DraI TTTAAA 1 cut(s) 3016
EaeI YGGCCR 1 cut(s) 1537
Eam1104I CTCTTC 4 cut(s) 530, 1953, 2046, 2641
EarI CTCTTC 4 cut(s) 530, 1953, 2046, 2641
EciI GGCGGA 1 cut(s) 540
Ecl136II GAGCTC 2 cut(s) 540, 2848
Eco130I CCWWGG 1 cut(s) 558
Eco24I GRGCYC 3 cut(s) 542, 566, 2850
Eco31I GGTCTC 4 cut(s) 93, 386, 968, 1448
Eco32I GATATC 3 cut(s) 2391, 2556, 3499
Eco53kI GAGCTC 2 cut(s) 540, 2848
Eco57I CTGAAG 6 cut(s) 149, 501, 518, 1353, 1974, 2319
Eco81I CCTNAGG 1 cut(s) 3687
EcoICRI GAGCTC 2 cut(s) 540, 2848
EcoRI GAATTC 1 cut(s) 2263
EcoRII CCWGG 5 cut(s) 1534, 1788, 2396, 3156, 3404
EcoRV GATATC 3 cut(s) 2391, 2556, 3499
EcoT14I CCWWGG 1 cut(s) 558
EcoT22I ATGCAT 5 cut(s) 1504, 1721, 2977, 3437, 3552
EcoT38I GRGCYC 3 cut(s) 542, 566, 2850
ErhI CCWWGG 1 cut(s) 558
Esp3I CGTCTC 2 cut(s) 716, 3638
FalI AAGNNNNNCTT 8 cut(s) 380, 412, 2144, 2176, 2235, 2267, 3111, 3143
FaqI GGGAC 3 cut(s) 1330, 1591, 3607
FauI CCCGC 1 cut(s) 2378
FauNDI CATATG 2 cut(s) 2440, 2977
FbaI TGATCA 2 cut(s) 1275, 2980
FblI GTMKAC 4 cut(s) 493, 940, 2683, 3235
Fnu4HI GCNGC 8 cut(s) 90, 295, 636, 1382, 1680, 1768, 1929, 3693
FriOI GRGCYC 3 cut(s) 542, 566, 2850
Fsp4HI GCNGC 8 cut(s) 90, 295, 636, 1382, 1680, 1768, 1929, 3693
GlaI GCGC 1 cut(s) 3524
GluI GCNGC 8 cut(s) 90, 295, 636, 1382, 1680, 1768, 1929, 3693
GsaI CCCAGC 1 cut(s) 1968
GsuI CTGGAG 1 cut(s) 2598
HaeIII GGCC 4 cut(s) 149, 1539, 2513, 3404
HhaI GCGC 1 cut(s) 3525
Hin1I GRCGYC 1 cut(s) 709
Hin6I GCGC 1 cut(s) 3523
HinP1I GCGC 1 cut(s) 3523
HincII GTYRAC 1 cut(s) 1321
HindII GTYRAC 1 cut(s) 1321
HindIII AAGCTT 2 cut(s) 2913, 3744
HpaI GTTAAC 1 cut(s) 1321
HphI GGTGA 5 cut(s) 997, 1601, 2144, 2575, 3080
Hpy99I CGWCG 2 cut(s) 711, 864
HpyCH4IV ACGT 5 cut(s) 709, 2535, 2680, 3075, 3631
HpyF10VI GCNNNNNNNGC 8 cut(s) 291, 370, 965, 1776, 2921, 3484, 3522, 3752
HpySE526I ACGT 5 cut(s) 709, 2535, 2680, 3075, 3631
Hsp92I GRCGYC 1 cut(s) 709
HspAI GCGC 1 cut(s) 3523
Ksp22I TGATCA 2 cut(s) 1275, 2980
KspAI GTTAAC 1 cut(s) 1321
LguI GCTCTTC 2 cut(s) 530, 2046
LmnI GCTCC 7 cut(s) 370, 511, 1361, 1846, 1992, 2067, 2357
Lsp1109I GCAGC 8 cut(s) 76, 281, 647, 1393, 1691, 1779, 1915, 3704
MaeII ACGT 5 cut(s) 709, 2535, 2680, 3075, 3631
MbiI CCGCTC 1 cut(s) 74
MfeI CAATTG 1 cut(s) 1734
MflI RGATCY 5 cut(s) 1899, 2102, 2157, 2692, 2714
MhlI GDGCHC 7 cut(s) 542, 566, 683, 2230, 2850, 3193, 3352
MlsI TGGCCA 1 cut(s) 1539
MluNI TGGCCA 1 cut(s) 1539
MlyI GAGTC 6 cut(s) 199, 1095, 1352, 1781, 2589, 3212
MmeI TCCRAC 7 cut(s) 217, 348, 429, 730, 1078, 1216, 1824
Mox20I TGGCCA 1 cut(s) 1539
Mph1103I ATGCAT 5 cut(s) 1504, 1721, 2977, 3437, 3552
MscI TGGCCA 1 cut(s) 1539
MslI CAYNNNNRTG 3 cut(s) 686, 1995, 3555
Msp20I TGGCCA 1 cut(s) 1539
MspCI CTTAAG 1 cut(s) 1922
MspR9I CCNGG 5 cut(s) 1536, 1790, 2398, 3158, 3406
MunI CAATTG 1 cut(s) 1734
Mva1269I GAATGC 1 cut(s) 523
MvaI CCWGG 5 cut(s) 1536, 1790, 2398, 3158, 3406
MvnI CGCG 2 cut(s) 70, 420
MwoI GCNNNNNNNGC 8 cut(s) 291, 370, 965, 1776, 2921, 3484, 3522, 3752
NdeI CATATG 2 cut(s) 2440, 2977
NlaIV GGNNCC 6 cut(s) 1357, 1878, 2063, 2716, 3330, 3403
NmuCI GTSAC 4 cut(s) 1421, 2132, 2138, 2581
NsiI ATGCAT 5 cut(s) 1504, 1721, 2977, 3437, 3552
NspI RCATGY 5 cut(s) 1117, 1723, 2350, 2490, 3491
PaeI GCATGC 1 cut(s) 3491
PciI ACATGT 1 cut(s) 2346
PciSI GCTCTTC 2 cut(s) 530, 2046
PctI GAATGC 1 cut(s) 523
PflFI GACNNNGTC 1 cut(s) 1007
PflMI CCANNNNNTGG 2 cut(s) 746, 2537
PkrI GCNGC 8 cut(s) 91, 296, 637, 1383, 1681, 1769, 1930, 3694
PleI GAGTC 6 cut(s) 198, 1095, 1351, 1780, 2588, 3212
PpsI GAGTC 6 cut(s) 198, 1095, 1351, 1780, 2588, 3212
PscI ACATGT 1 cut(s) 2346
Psp124BI GAGCTC 2 cut(s) 542, 2850
Psp1406I AACGTT 1 cut(s) 2535
Psp6I CCWGG 5 cut(s) 1534, 1788, 2396, 3156, 3404
PspFI CCCAGC 1 cut(s) 1964
PspGI CCWGG 5 cut(s) 1534, 1788, 2396, 3156, 3404
PspN4I GGNNCC 6 cut(s) 1357, 1878, 2063, 2716, 3330, 3403
PspPI GGNCC 2 cut(s) 2512, 3402
PstI CTGCAG 2 cut(s) 366, 3167
PsuI RGATCY 5 cut(s) 1899, 2102, 2157, 2692, 2714
PsyI GACNNNGTC 1 cut(s) 1007
RsaI GTAC 7 cut(s) 759, 1248, 1407, 1565, 2364, 2408, 2569
RsaNI GTAC 7 cut(s) 758, 1247, 1406, 1564, 2363, 2407, 2568
RseI CAYNNNNRTG 3 cut(s) 686, 1995, 3555
SacI GAGCTC 2 cut(s) 542, 2850
SapI GCTCTTC 2 cut(s) 530, 2046
SatI GCNGC 8 cut(s) 90, 295, 636, 1382, 1680, 1768, 1929, 3693
Sau96I GGNCC 2 cut(s) 2512, 3402
ScaI AGTACT 1 cut(s) 1248
SchI GAGTC 6 cut(s) 199, 1095, 1352, 1781, 2589, 3212
ScrFI CCNGG 5 cut(s) 1536, 1790, 2398, 3158, 3406
SduI GDGCHC 7 cut(s) 542, 566, 683, 2230, 2850, 3193, 3352
SfcI CTRYAG 2 cut(s) 362, 3163
SmiI ATTTAAAT 1 cut(s) 3016
SmiMI CAYNNNNRTG 3 cut(s) 686, 1995, 3555
SmlI CTYRAG 8 cut(s) 428, 1449, 1816, 1922, 2033, 2370, 2910, 3637
SmoI CTYRAG 8 cut(s) 428, 1449, 1816, 1922, 2033, 2370, 2910, 3637
SpeI ACTAGT 1 cut(s) 625
SphI GCATGC 1 cut(s) 3491
SsiI CCGC 4 cut(s) 74, 418, 525, 2385
SspI AATATT 3 cut(s) 997, 2205, 2839
SstI GAGCTC 2 cut(s) 542, 2850
StyD4I CCNGG 5 cut(s) 1534, 1788, 2396, 3156, 3404
StyI CCWWGG 1 cut(s) 558
SwaI ATTTAAAT 1 cut(s) 3016
TaiI ACGT 5 cut(s) 712, 2538, 2683, 3078, 3634
TaqI TCGA 4 cut(s) 1104, 1167, 2146, 3276
TatI WGTACW 4 cut(s) 1246, 1405, 1563, 2567
TseFI GTSAC 4 cut(s) 1421, 2132, 2138, 2581
TseI GCWGC 8 cut(s) 89, 294, 635, 1381, 1679, 1767, 1928, 3692
Tsp45I GTSAC 4 cut(s) 1421, 2132, 2138, 2581
TspGWI ACGGA 1 cut(s) 1348
Tth111I GACNNNGTC 1 cut(s) 1007
Van91I CCANNNNNTGG 2 cut(s) 746, 2537
Vha464I CTTAAG 1 cut(s) 1922
XbaI TCTAGA 2 cut(s) 1489, 3214
XceI RCATGY 5 cut(s) 1117, 1723, 2350, 2490, 3491
XmiI GTMKAC 4 cut(s) 493, 940, 2683, 3235
ZraI GACGTC 1 cut(s) 710
ZrmI AGTACT 1 cut(s) 1248
Zsp2I ATGCAT 5 cut(s) 1504, 1721, 2977, 3437, 3552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.