Rw0G008650
WRKY Family

WRKY Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00331
OG0002237
Physical Location & Seq
Reverse (-)
61381 .. 65226
3846 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G008650.1

Sequence Viewer

Length: 1431 bp
ATGAAGGTCATGTTGGAGAAGATACTGCAGATGATTGAAGATCCTGAAGTGAAGGGTCTACTGAAGAAATTAGTGGAGCAGAATGCAGACGGATCAATGAAGAACTCGACCTCAACCTCAACCTTGGGCTCAGGAAGTTGCACAGAAAATGAAGGGAATTGCGTAACAAAAGTGCCATCATCAAAATCACTGGTAGAAGCAGCATTTCGGGTACTACTTAATGCCGTTATAGAAGTGAAGGAGAGCACTATGATGTTTAGACCTCTCCTCCGACGTCTCAAATCTACTCTAGACTATTTACTGCCATTTATTGAAGATATGGTACAATGTAACAAGGCATTAGATCTTCCAAAAGAGGAACTAGAAAATATGAGAATACAGATGGAGAATGGTGTAATGCTTGGCAAGTGCTTACATGTTCGTCGGTGGACAAGGAACAAAAAGTATATATACACCAACCAACTTTTTGAACTGGATGAATATCTTCAAGGGCAGTTAAGTATACTGCTAGTTCAGATAGCAAGGGATGTGAGGGAGACCTCGGTGTTGATAAGGAATATTAAGGAGGTGGTCGGGAGAAATAAAAGGAATGGTATGGCAGAAAATCAACTTGAATATAAAGGTTGTTGTAATGTATCTGAACCTCCACTCCCTACTGTTGTATTCGTAACAAACATGCAAGGAACAAGGGATGTGATGATGGAGACGTTGGTTTCTGTAAAGAATATCGAGGAGGGGGTCAAAGGAATGGAAGGGAGAAGTACGGTAGATGAAGTATCTGAAACTTTATCGCCTACTGTTGGAATTGAAGTACTCAACATGCAGGGAACAAAGGATGTGATCAAGGAGACACAGGCTTCAACAGAGAATACAAATGCAGAGGTCAATCTAATTGAAGGGAGAGCTGAGACCCAGCTTGGCTCCATTGCATCTTCAGAGGGACAACAGCTGACAGAGGGAATCAGTGAAGTACATGGAAGAGGTCACTGTGAACTGGAAAATTCAGTATCTCAAGAGACCTTTTCTCCATGCCTTCCTAGTTCCATCCCTCTAGAGTTAAATGCATTGAAGAGGGAAAATGTATTTCCTGAGAGACCTGGCCAGCCTGACTGCCTTGATAAGGAGTACAAAATCAACTCTACAGTGAAAGAGCCTGTTTCCTTTAAGGTTGATCATTCAGTGTACATTGAACTATGGAAGTCGGTTGATAGAATTTCAAAGATATTTCCCGACATAGAAGCAGCAAGAACTCGAAGCGCAACGGGAATACAGGCTCTATGCATGTTGAATCGTGCAATTGAGAAAGCCAAGCAACTTGTTCAGTATTGCAGCGAGTCTAGCAAACTCGACCCGGCAGTTTTTTTTCCTGTCATATGCTGGGTGCATTCTGTTCAATATGAGCTCTATCGCTCCTTGTCATCCTATGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

476

Amino Acids

53.42

Weight (kDa)

5.44

Isoelectric Point (pI)

45.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 65 - 183 1.2e-20 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 277
AccI GTMKAC 2 cut(s) 58, 502
AclWI GGATC 2 cut(s) 35, 100
AcoI YGGCCR 1 cut(s) 1099
AcsI RAATTY 2 cut(s) 1000, 1212
AcuI CTGAAG 3 cut(s) 66, 83, 918
AcyI GRCGYC 1 cut(s) 274
AfaI GTAC 7 cut(s) 213, 324, 763, 813, 972, 1127, 1184
AfiI CCNNNNNNNGG 2 cut(s) 800, 1120
AflIII ACRYGT 1 cut(s) 415
AjnI CCWGG 1 cut(s) 1096
AjuI GAANNNNNNNTTGG 1 cut(s) 28
AloI GAACNNNNNNTCC 2 cut(s) 633, 665
AluBI AGCT 4 cut(s) 905, 916, 949, 1402
AluI AGCT 4 cut(s) 905, 916, 949, 1402
Alw21I GWGCWC 2 cut(s) 248, 1404
Alw26I GTCTC 7 cut(s) 281, 530, 698, 842, 902, 1010, 1087
AlwI GGATC 2 cut(s) 35, 100
AoxI GGCC 1 cut(s) 1099
ApeKI GCWGC 3 cut(s) 200, 1241, 1329
ApoI RAATTY 2 cut(s) 1000, 1212
Asp700I GAANNNNTTC 1 cut(s) 483
AspLEI GCGC 1 cut(s) 1259
AsuC2I CCSGG 1 cut(s) 1352
BalI TGGCCA 1 cut(s) 1101
BanII GRGCYC 2 cut(s) 131, 1404
BarI GAAGNNNNNNTAC 2 cut(s) 306, 338
Bbv12I GWGCWC 2 cut(s) 248, 1404
BbvI GCAGC 3 cut(s) 212, 1253, 1341
BccI CCATC 4 cut(s) 184, 376, 694, 1052
BceAI ACGGC 1 cut(s) 209
BciT130I CCWGG 1 cut(s) 1098
BclI TGATCA 2 cut(s) 840, 1171
BcnI CCSGG 1 cut(s) 1352
BcoDI GTCTC 7 cut(s) 281, 530, 698, 842, 902, 1010, 1087
BfaI CTAG 6 cut(s) 290, 362, 509, 1038, 1052, 1338
BfmI CTRYAG 2 cut(s) 26, 1140
BglII AGATCT 1 cut(s) 343
BisI GCNGC 3 cut(s) 201, 1242, 1330
BlsI GCNGC 3 cut(s) 202, 1243, 1331
BmcAI AGTACT 1 cut(s) 813
Bme1390I CCNGG 2 cut(s) 1098, 1352
BmiI GGNNCC 1 cut(s) 922
BmrFI CCNGG 2 cut(s) 1098, 1352
BmsI GCATC 1 cut(s) 938
Bpu10I CCTNAGC 1 cut(s) 130
BpuEI CTTGAG 1 cut(s) 996
BpuMI CCSGG 1 cut(s) 1352
BsaBI GATNNNNATC 1 cut(s) 480
BsaHI GRCGYC 1 cut(s) 274
BsaI GGTCTC 4 cut(s) 530, 902, 1010, 1087
BsaJI CCNNGG 2 cut(s) 123, 540
BsaXI ACNNNNNCTCC 6 cut(s) 252, 282, 633, 663, 1009, 1039
Bsc4I CCNNNNNNNGG 2 cut(s) 800, 1120
Bse1I ACTGG 3 cut(s) 195, 477, 999
Bse3DI GCAATG 1 cut(s) 924
Bse8I GATNNNNATC 1 cut(s) 480
BseBI CCWGG 1 cut(s) 1098
BseDI CCNNGG 2 cut(s) 123, 540
BseGI GGATG 6 cut(s) 481, 532, 697, 841, 1044, 1418
BseJI GATNNNNATC 1 cut(s) 480
BseLI CCNNNNNNNGG 2 cut(s) 800, 1120
BseMI GCAATG 1 cut(s) 924
BseMII CTCAG 3 cut(s) 144, 897, 1080
BseNI ACTGG 3 cut(s) 195, 477, 999
BseRI GAGGAG 2 cut(s) 257, 746
BseXI GCAGC 3 cut(s) 212, 1253, 1341
BseYI CCCAGC 2 cut(s) 912, 1377
BshFI GGCC 1 cut(s) 1101
BsiHKAI GWGCWC 2 cut(s) 248, 1404
BsiSI CCGG 1 cut(s) 1352
BslFI GGGAC 1 cut(s) 954
BslI CCNNNNNNNGG 2 cut(s) 800, 1120
BsmAI GTCTC 7 cut(s) 281, 530, 698, 842, 902, 1010, 1087
BsmBI CGTCTC 2 cut(s) 281, 698
BsmFI GGGAC 1 cut(s) 954
BsmI GAATGC 2 cut(s) 88, 1384
BsnI GGCC 1 cut(s) 1101
Bso31I GGTCTC 4 cut(s) 530, 902, 1010, 1087
Bsp1286I GDGCHC 3 cut(s) 131, 248, 1404
Bsp1407I TGTACA 1 cut(s) 1182
Bsp143I GATC 5 cut(s) 40, 92, 343, 840, 1171
BspANI GGCC 1 cut(s) 1101
BspCNI CTCAG 3 cut(s) 143, 898, 1081
BspLI GGNNCC 1 cut(s) 922
BspMAI CTGCAG 1 cut(s) 30
BspPI GGATC 2 cut(s) 35, 100
BspTNI GGTCTC 4 cut(s) 530, 902, 1010, 1087
BsrDI GCAATG 1 cut(s) 924
BsrGI TGTACA 1 cut(s) 1182
BsrI ACTGG 3 cut(s) 195, 477, 999
BssECI CCNNGG 2 cut(s) 123, 540
BssMI GATC 5 cut(s) 40, 92, 343, 840, 1171
BssNAI GTATAC 1 cut(s) 503
BssNI GRCGYC 1 cut(s) 274
BssT1I CCWWGG 1 cut(s) 123
Bst1107I GTATAC 1 cut(s) 503
Bst2UI CCWGG 1 cut(s) 1098
Bst4CI ACNGT 5 cut(s) 658, 766, 799, 989, 1144
Bst6I CTCTTC 2 cut(s) 973, 1064
BstACI GRCGYC 1 cut(s) 274
BstAUI TGTACA 1 cut(s) 1182
BstC8I GCNNGC 1 cut(s) 1103
BstDEI CTNAG 3 cut(s) 130, 906, 1089
BstENI CCTNNNNNAGG 1 cut(s) 1118
BstF5I GGATG 6 cut(s) 481, 532, 697, 841, 1044, 1418
BstHHI GCGC 1 cut(s) 1259
BstKTI GATC 5 cut(s) 43, 95, 346, 843, 1174
BstMAI GTCTC 7 cut(s) 281, 530, 698, 842, 902, 1010, 1087
BstMBI GATC 5 cut(s) 40, 92, 343, 840, 1171
BstMWI GCNNNNNNNGC 1 cut(s) 1338
BstNI CCWGG 1 cut(s) 1098
BstNSI RCATGY 4 cut(s) 419, 679, 823, 1285
BstSCI CCNGG 2 cut(s) 1096, 1350
BstSFI CTRYAG 2 cut(s) 26, 1140
BstV1I GCAGC 3 cut(s) 212, 1253, 1341
BstX2I RGATCY 2 cut(s) 40, 343
BstYI RGATCY 2 cut(s) 40, 343
BstZ17I GTATAC 1 cut(s) 503
BsuRI GGCC 1 cut(s) 1101
BtsCI GGATG 6 cut(s) 481, 532, 697, 841, 1044, 1418
BtsIMutI CAGTG 5 cut(s) 188, 970, 985, 1149, 1185
Cac8I GCNNGC 1 cut(s) 1103
CfoI GCGC 1 cut(s) 1259
Csp6I GTAC 7 cut(s) 212, 323, 762, 812, 971, 1126, 1183
CviAII CATG 7 cut(s) 10, 416, 676, 820, 974, 1029, 1282
CviQI GTAC 7 cut(s) 212, 323, 762, 812, 971, 1126, 1183
DdeI CTNAG 3 cut(s) 130, 906, 1089
DpnI GATC 5 cut(s) 42, 94, 345, 842, 1173
DpnII GATC 5 cut(s) 40, 92, 343, 840, 1171
EaeI YGGCCR 1 cut(s) 1099
Eam1104I CTCTTC 2 cut(s) 973, 1064
EarI CTCTTC 2 cut(s) 973, 1064
Ecl136II GAGCTC 1 cut(s) 1402
Eco130I CCWWGG 1 cut(s) 123
Eco24I GRGCYC 2 cut(s) 131, 1404
Eco31I GGTCTC 4 cut(s) 530, 902, 1010, 1087
Eco53kI GAGCTC 1 cut(s) 1402
Eco57I CTGAAG 3 cut(s) 66, 83, 918
EcoICRI GAGCTC 1 cut(s) 1402
EcoNI CCTNNNNNAGG 1 cut(s) 1118
EcoRII CCWGG 1 cut(s) 1096
EcoT14I CCWWGG 1 cut(s) 123
EcoT22I ATGCAT 2 cut(s) 1066, 1283
EcoT38I GRGCYC 2 cut(s) 131, 1404
ErhI CCWWGG 1 cut(s) 123
Esp3I CGTCTC 2 cut(s) 281, 698
FaeI CATG 7 cut(s) 13, 419, 679, 823, 977, 1032, 1285
FaqI GGGAC 1 cut(s) 954
FatI CATG 7 cut(s) 9, 415, 675, 819, 973, 1028, 1281
FauNDI CATATG 1 cut(s) 1373
FbaI TGATCA 2 cut(s) 840, 1171
FblI GTMKAC 2 cut(s) 58, 502
Fnu4HI GCNGC 3 cut(s) 201, 1242, 1330
FokI GGATG 6 cut(s) 488, 539, 704, 848, 1031, 1405
FriOI GRGCYC 2 cut(s) 131, 1404
Fsp4HI GCNGC 3 cut(s) 201, 1242, 1330
FspBI CTAG 6 cut(s) 290, 362, 509, 1038, 1052, 1338
GlaI GCGC 1 cut(s) 1258
GluI GCNGC 3 cut(s) 201, 1242, 1330
GsaI CCCAGC 2 cut(s) 916, 1381
HaeIII GGCC 1 cut(s) 1101
HapII CCGG 1 cut(s) 1352
HhaI GCGC 1 cut(s) 1259
Hin1I GRCGYC 1 cut(s) 274
Hin1II CATG 7 cut(s) 13, 419, 679, 823, 977, 1032, 1285
Hin6I GCGC 1 cut(s) 1257
HinP1I GCGC 1 cut(s) 1257
HinfI GANTC 3 cut(s) 960, 1288, 1334
HpaII CCGG 1 cut(s) 1352
Hpy166II GTNNAC 5 cut(s) 59, 429, 503, 992, 1183
Hpy188I TCNGA 5 cut(s) 272, 516, 640, 781, 937
Hpy188III TCNNGA 8 cut(s) 44, 132, 290, 574, 1013, 1052, 1088, 1229
Hpy8I GTNNAC 5 cut(s) 59, 429, 503, 992, 1183
Hpy99I CGWCG 2 cut(s) 276, 426
HpyAV CCTTC 6 cut(s) 46, 146, 232, 746, 890, 1043
HpyCH4III ACNGT 5 cut(s) 658, 766, 799, 989, 1144
HpyCH4IV ACGT 2 cut(s) 274, 707
HpyF10VI GCNNNNNNNGC 1 cut(s) 1338
HpyF3I CTNAG 3 cut(s) 130, 906, 1089
HpySE526I ACGT 2 cut(s) 274, 707
Hsp92I GRCGYC 1 cut(s) 274
Hsp92II CATG 7 cut(s) 13, 419, 679, 823, 977, 1032, 1285
HspAI GCGC 1 cut(s) 1257
Ksp22I TGATCA 2 cut(s) 840, 1171
Kzo9I GATC 5 cut(s) 40, 92, 343, 840, 1171
LmnI GCTCC 3 cut(s) 76, 926, 1415
Lsp1109I GCAGC 3 cut(s) 212, 1253, 1341
LweI GCATC 1 cut(s) 938
MaeI CTAG 6 cut(s) 290, 362, 509, 1038, 1052, 1338
MaeII ACGT 2 cut(s) 274, 707
MaeIII GTNAC 4 cut(s) 163, 329, 667, 983
MalI GATC 5 cut(s) 42, 94, 345, 842, 1173
MboI GATC 5 cut(s) 40, 92, 343, 840, 1171
MfeI CAATTG 1 cut(s) 1296
MflI RGATCY 2 cut(s) 40, 343
MhlI GDGCHC 3 cut(s) 131, 248, 1404
MlsI TGGCCA 1 cut(s) 1101
MluCI AATT 7 cut(s) 68, 157, 804, 891, 1000, 1212, 1296
MluNI TGGCCA 1 cut(s) 1101
MlyI GAGTC 1 cut(s) 1343
MmeI TCCRAC 2 cut(s) 295, 781
Mox20I TGGCCA 1 cut(s) 1101
Mph1103I ATGCAT 2 cut(s) 1066, 1283
MroXI GAANNNNTTC 1 cut(s) 483
MscI TGGCCA 1 cut(s) 1101
MseI TTAA 5 cut(s) 219, 497, 561, 1058, 1164
MslI CAYNNNNRTG 1 cut(s) 251
Msp20I TGGCCA 1 cut(s) 1101
MspA1I CMGCKG 1 cut(s) 949
MspI CCGG 1 cut(s) 1352
MspR9I CCNGG 2 cut(s) 1098, 1352
MunI CAATTG 1 cut(s) 1296
Mva1269I GAATGC 2 cut(s) 88, 1384
MvaI CCWGG 1 cut(s) 1098
MwoI GCNNNNNNNGC 1 cut(s) 1338
NciI CCSGG 1 cut(s) 1352
NdeI CATATG 1 cut(s) 1373
NdeII GATC 5 cut(s) 40, 92, 343, 840, 1171
NlaIII CATG 7 cut(s) 13, 419, 679, 823, 977, 1032, 1285
NlaIV GGNNCC 1 cut(s) 922
NmuCI GTSAC 1 cut(s) 983
NsiI ATGCAT 2 cut(s) 1066, 1283
NspI RCATGY 4 cut(s) 419, 679, 823, 1285
PciI ACATGT 1 cut(s) 415
PctI GAATGC 2 cut(s) 88, 1384
PdmI GAANNNNTTC 1 cut(s) 483
PfeI GAWTC 2 cut(s) 960, 1288
PkrI GCNGC 3 cut(s) 202, 1243, 1331
PleI GAGTC 1 cut(s) 1342
PpsI GAGTC 1 cut(s) 1342
PscI ACATGT 1 cut(s) 415
Psp124BI GAGCTC 1 cut(s) 1404
Psp6I CCWGG 1 cut(s) 1096
PspFI CCCAGC 2 cut(s) 912, 1377
PspGI CCWGG 1 cut(s) 1096
PspN4I GGNNCC 1 cut(s) 922
PstI CTGCAG 1 cut(s) 30
PsuI RGATCY 2 cut(s) 40, 343
PvuII CAGCTG 1 cut(s) 949
RsaI GTAC 7 cut(s) 213, 324, 763, 813, 972, 1127, 1184
RsaNI GTAC 7 cut(s) 212, 323, 762, 812, 971, 1126, 1183
RseI CAYNNNNRTG 1 cut(s) 251
SacI GAGCTC 1 cut(s) 1404
SaqAI TTAA 5 cut(s) 219, 497, 561, 1058, 1164
SatI GCNGC 3 cut(s) 201, 1242, 1330
Sau3AI GATC 5 cut(s) 40, 92, 343, 840, 1171
ScaI AGTACT 1 cut(s) 813
SchI GAGTC 1 cut(s) 1343
ScrFI CCNGG 2 cut(s) 1098, 1352
SduI GDGCHC 3 cut(s) 131, 248, 1404
SfaNI GCATC 1 cut(s) 938
SfcI CTRYAG 2 cut(s) 26, 1140
SmiMI CAYNNNNRTG 1 cut(s) 251
SmlI CTYRAG 1 cut(s) 1011
SmoI CTYRAG 1 cut(s) 1011
Sse9I AATT 7 cut(s) 68, 157, 804, 891, 1000, 1212, 1296
SspI AATATT 1 cut(s) 559
SspMI CTAG 6 cut(s) 290, 362, 509, 1038, 1052, 1338
SstI GAGCTC 1 cut(s) 1404
StyD4I CCNGG 2 cut(s) 1096, 1350
StyI CCWWGG 1 cut(s) 123
TaaI ACNGT 5 cut(s) 658, 766, 799, 989, 1144
TaiI ACGT 2 cut(s) 277, 710
TaqI TCGA 4 cut(s) 107, 729, 1252, 1347
TasI AATT 7 cut(s) 68, 157, 804, 891, 1000, 1212, 1296
TatI WGTACW 4 cut(s) 811, 970, 1125, 1182
TfiI GAWTC 2 cut(s) 960, 1288
Tru1I TTAA 5 cut(s) 219, 497, 561, 1058, 1164
Tru9I TTAA 5 cut(s) 219, 497, 561, 1058, 1164
TscAI CASTG 5 cut(s) 195, 970, 992, 1149, 1185
TseFI GTSAC 1 cut(s) 983
TseI GCWGC 3 cut(s) 200, 1241, 1329
Tsp45I GTSAC 1 cut(s) 983
TspDTI ATGAA 5 cut(s) 17, 113, 165, 492, 786
TspGWI ACGGA 1 cut(s) 105
TspRI CASTG 5 cut(s) 195, 970, 992, 1149, 1185
XagI CCTNNNNNAGG 1 cut(s) 1118
XapI RAATTY 2 cut(s) 1000, 1212
XbaI TCTAGA 2 cut(s) 289, 1051
XceI RCATGY 4 cut(s) 419, 679, 823, 1285
XmiI GTMKAC 2 cut(s) 58, 502
XmnI GAANNNNTTC 1 cut(s) 483
XspI CTAG 6 cut(s) 290, 362, 509, 1038, 1052, 1338
ZraI GACGTC 1 cut(s) 275
ZrmI AGTACT 1 cut(s) 813
Zsp2I ATGCAT 2 cut(s) 1066, 1283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.