RchiOBHm_Chr2g0104731

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0002237
Physical Location & Seq
Reverse (-)
16007403 .. 16014393
6991 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47898

Sequence Viewer

Length: 3831 bp
ATGGCATTATCATTTGCACTGGATTTAATCGGAGGACAAGTATTTGAGCTGTTGTCTGCAAGTGTTACGCGAGCGGTGGACAGGAAAGCTGCGTTTGGAGACCTTCTCCAAGATATGAAATACACACTGAAGTCCTTGAAAAGACAGGCCATAGTGATTCAACAGATACAAGAGTATAATCTGGAACTGAGTCTCCCAAATGATGAAATGATTGCTCTTGTAACACAAATGACAGTTGGAAGACAACTTGTTGATATGTTGTCAGGGATTAGATTTCGTTTGTGCAACTTCTGCTGCATAAATGATTTGGCTGACCAACTTAAAGAATTGAATAGTTCTCTTGAAAGTCTGTTTGACAACCTGCAGTTGGAGCAAGTAAGGGATATTAAGGAGACCTTGAAATTGTCAAGGCAAAACCGCGATGTTCTTGAGAAAATGAAGATCATGTTGGAGAAGATAATGCAGATGATTGATGATCCTGAAGTGAAGGGTCTACTGAAGAAGTTAGTGGAGCAGAATGCAGGCGGATCAATGAAGAGCTCGCCCTCAACCTCAACCTTGGGCTCAGGAAGTTGCACAGAAAATGAAGGGAATTGCGAAACACAAGTGCCGTCATCAAAAGCACTAGTAGAAGCAGCATTTCGGGTGCTACTTAATGTCGTTATAGAAGTGAAGGAGAGCACTATTATGTTTAGACCTCTCCTCCGACGTCTCAAATCTACACTAGACTATTTACTGCCATTGATTGGAGATATGGTACAATGTAACAAGGCATTAGATCGCCCAAAAGAGGAACTAGAAAATATGAGAATACAAATGGAGAATGGTGTAGTGCTTGTTGGCAAGTGCTTAAATGTTCGTCGGTGGACAAGAAACAAAAAGTATATATACACCAACCAACTTTTTGAATTGAATGATTATCTTCAAGAGCAGTTAAGTATACTGCTAGTGGAGATAGCAAGGGATGCGAGGGAGACCTCGGTGGTGATAAGGAATATTAAGGACATGGTCAAGAGAGATGAAAGGAGTGGTATGGCAGAAAATCAACTTGAATATAAAGGTTTAATTTGTAATGTATCTGAACCTCCATTGCCTACTGTTGGACTCGAAACAAACATGCAAGGAACAAGGGATGTGATGATGGAGACATTGGTTTCTGTAAAGAATATCGAGGAGGGGGTCAAGGGAATGGAAGGGAGAATTATAGATGATGTATCTGAAACTCTGTCGCCTATTGTTGGAAGTGAAGTACTCAACATGCAGGGAACAAAGGATGTGATCAAGGAGACACAGGCTTCAACAGAGAATACAAATCCAGAGGTCAACCTAATTGAAGGCAGAGCTGAGTCCCAGTTTGGCTCCGTTGCATCTTCAGAGAGCCAGCAGCAAACAGAAGGAATCAGTAAAGTACATGGAACTTTAGGTCACTGTGAACTGGAAAATTCAGTATCTCAAGAGACCTTTTCACTATACCTTCCTGATTCCATCCCTCTAGAGTTCAATGCATTGAAGAAGGAAGCTGTATTTCCTGACAGACCTGGCCAACCTGACTGTCTTGACAAGGAGTACAAAATCAACGGGACAGTGAGAAAGCCTATTTCCTTTAAGGTTCACTGTTCAATGTGCATTCAACTTTGGAAGTTGGTGGAGAGAATTTCGAAGATATTTCCCGACATAGAAGCAGCAAGACCTCGTTGCTCAACGGGAATACAGGCTCTATGCATGTTGAATCGTGCAATTGAGAAAGCCAAGCAACTTGTTCAGTATTGCAGCGAGTCTAGCAAACTCTACCTGGCGTTAACAGGGGATGTGATAGTCTCAAGATGTCAAAAATCAAGGAACTTGTTGGAGCAAAGTTTGTGCCAGATTCAGAGTTGGGTTCCAGTGATTTTGGTGGCAGAGATCTCTAAAATAGTTGATGATCTTAAGGCTGCAACATTTAGACTGCATCCATCTGAAGAAGAGGCTGGGAAGGTCATCCGAGAATTGCTCCACCAAGATGCTTCTGCATCAAATTCAATAAAAGATTCTGAAATCAAGGCTCTTCAACTTGCATCTTTGAGGCTCCATATTACCACATCAAAGGAAGTCTTAGTAGAGAAAAGATCTATTAAGAAGTTGCTACAAAATGTTGGTGACAGTGACCCTCGAAAAAAGCAGATCTTGACATATTTCTTGTATCTATTGAAGAAGTATGGAAACTTAATATTGGTAAAGCAAACAGAGAGTTCCCCTGTGTGGCAACAAGGATCACTTGCTTCTGAGAATTCTGGCAACGACTTTGGATATAATCAGTGTGCTGAAGTTGAACCTCACACTGGTTATGGGGAATATGAGGCACAAAGTAACATGTTGCTTGGAGCTGTACCTCCTCAAGAATTTTTGTGCGGGATATCTTCCAGGTTGATGTACGATCCTGTTCTCATTGCTTCTGGACAAACATATGAAAGGATGTGGATACAGAAGTGGTTTGATGAGGGAAATGATACATGCCCAAAAACTAATACCAAACTGGCCCACCTTTCATTGACTCCAAACGTTGGCATGACGGAGTTGATATCAAGGTGGTGTAAGAAGTATGGAGTCACCATCCCCAACCCAAGTATGCAACCAGAAGCACTTAGCTCATGGGAAAACTCTTCCACTTCCATTGCTAGTTTAGGCAGTTCTATGAATGATCTACGTCTACAAATGGATCTCAGCACTATGTCATTTGGATCCTTAGATACCAGTTTCAATTCTAAGACAGAAGATGGCTCAAATCTGGTGCAGATTGATGATGATTCTCTGAAATACCGATCTGCTAAAAAATGCGAGACAGATTTGGAATTTCTATCTAATATTAGTGAGCTCAAATGGGATTCCCAACGCAAGGCTGTTGAAGATGTTAAAAACGATTTGAAACGCAATCCTCAAGCTTCTTATGCTATGTCCTCTAAGAATTTTGTTGAACCTCTCATCAAATTTTTGAGGGATGCATATGATCAGGATGATGTAGAGGCTCAGAGAGATGGATTTAAATTATTATCTACCTTTGTCAGCAAAAACAGAAACGGGATATTTTATTTCGGTGAAGACGTGTATAGTCTGTTAGTATCTTCTCTTGATTCAGAGGTAAAGGAAGAAGGTCTTGCCATAATGGAAATTCTGTCTGTCCACCAGGACTGCAGAGGTAACATCTCATCATCTGGTGCTCTTACATCCATTCTAAAACTTCTAGACTCTCAGAGCAGAAGTATACAAGGAAAAGCCCTTAAAATTTTGCACAATTTGTCCCTCGATAGAGATATTTGTTCAGAGATGGTATCTTTGGAGTGCATCCCAAAATTGGTTCCTTTCTTTGAAGACAGTGCTCTTACTGGAAACTGTATCGCTATATTGAAAAATCTAAGCGATTATGAAGGGGCCAGGATTTCTATTGCTGAAACTGATGGATGCATTGCTTATGTTATTTATGTCCTAGACACTGGCAGTAAAGAGCATCAAGAGCATGCTGTTGATATCCTTCTTTCTTTATGCTCTCAGCGCATTGATTATTGTAACTTGGTCATGCATGAGGGTGTAATTCCTGCTCTTGTCCTCTCATCTACCAATGGGACAGAGAGAACAAGGATGAACGCAATGGAAGTTCTACGTCTCTTGAGAGATATTGATTATGACGATGAGCAAGAATCTGCTGAACCTGAGACTGAGGCAGCTAGAGATGAGCTACCTAGAGACAATGGAAATCACTGGAACGGGAAGAAAGCTTCTAAGGCATCAGGATTTTTCGGAAAGAAGTTATCAATATTCTCAAAACCGAGTTCTCTTTCACCGAAAAAGAAGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KAP U-box

Protein Analysis

1276

Amino Acids

142.93

Weight (kDa)

5.29

Isoelectric Point (pI)

39.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 12 - 139 4.8e-11 Arabidopsis broad-spectrum mildew resistance protein RPW8
RPW8 PF05659 208 - 332 3e-23 Arabidopsis broad-spectrum mildew resistance protein RPW8
U-box PF04564 791 - 859 3.1e-16 U-box domain
ARM_PUB PF25598 979 - 1216 1.3e-12 Plant U-box protein ARM repeat region
KAP PF05804 1026 - 1161 2.2e-06 Kinesin-associated protein (KAP)
Arm_2 PF04826 1027 - 1127 8.7e-07 Armadillo-like
ARM_ARMC5 PF24768 1049 - 1211 3.3e-07 ARMC5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 712
Acc36I ACCTGC 1 cut(s) 369
AccB7I CCANNNNNTGG 2 cut(s) 746, 2540
AccBSI CCGCTC 1 cut(s) 74
AccI GTMKAC 4 cut(s) 493, 940, 2686, 3238
AccII CGCG 2 cut(s) 70, 420
AciI CCGC 4 cut(s) 74, 418, 525, 2388
AclI AACGTT 1 cut(s) 2538
AclWI GGATC 7 cut(s) 470, 535, 2257, 2408, 2703, 2712, 2725
AcoI YGGCCR 1 cut(s) 1540
AcuI CTGAAG 6 cut(s) 149, 501, 518, 1356, 1977, 2322
AcyI GRCGYC 1 cut(s) 709
AfaI GTAC 6 cut(s) 759, 1251, 1410, 1568, 2367, 2411
AflII CTTAAG 1 cut(s) 1925
AflIII ACRYGT 2 cut(s) 2349, 3078
AhlI ACTAGT 1 cut(s) 625
AjiI CACGTC 1 cut(s) 3079
AjnI CCWGG 5 cut(s) 1537, 1791, 2399, 3159, 3407
AjuI GAANNNNNNNTTGG 2 cut(s) 431, 463
AloI GAACNNNNNNTCC 2 cut(s) 177, 209
Alw21I GWGCWC 5 cut(s) 542, 683, 2853, 3196, 3355
AlwI GGATC 7 cut(s) 470, 535, 2257, 2408, 2703, 2712, 2725
AoxI GGCC 4 cut(s) 147, 1540, 2514, 3405
ApeKI GCWGC 8 cut(s) 89, 294, 635, 1384, 1682, 1770, 1931, 3695
AspLEI GCGC 1 cut(s) 3528
AspS9I GGNCC 2 cut(s) 2515, 3405
AsuHPI GGTGA 5 cut(s) 997, 2147, 2578, 3083, 3804
AsuII TTCGAA 1 cut(s) 1658
BaeI ACNNNNGTAYC 4 cut(s) 158, 191, 2450, 2483
BalI TGGCCA 1 cut(s) 1542
BamHI GGATCC 1 cut(s) 2717
BanII GRGCYC 3 cut(s) 542, 566, 2853
BbsI GAAGAC 3 cut(s) 247, 3081, 3351
Bbv12I GWGCWC 5 cut(s) 542, 683, 2853, 3196, 3355
BbvI GCAGC 8 cut(s) 76, 281, 647, 1396, 1694, 1782, 1918, 3707
BccI CCATC 8 cut(s) 1135, 1493, 1960, 2597, 2747, 3005, 3295, 3425
BceAI ACGGC 1 cut(s) 595
BciT130I CCWGG 5 cut(s) 1539, 1793, 2401, 3161, 3409
BciVI GTATCC 1 cut(s) 2451
BclI TGATCA 2 cut(s) 1278, 2983
BcuI ACTAGT 1 cut(s) 625
BfmI CTRYAG 2 cut(s) 362, 3166
BfrI CTTAAG 1 cut(s) 1925
BfuAI ACCTGC 1 cut(s) 369
BfuI GTATCC 1 cut(s) 2451
BglII AGATCT 3 cut(s) 1902, 2105, 2160
BisI GCNGC 8 cut(s) 90, 295, 636, 1385, 1683, 1771, 1932, 3696
BlsI GCNGC 8 cut(s) 91, 296, 637, 1386, 1684, 1772, 1933, 3697
BmcAI AGTACT 1 cut(s) 1251
Bme1390I CCNGG 5 cut(s) 1539, 1793, 2401, 3161, 3409
BmgBI CACGTC 1 cut(s) 3079
BmgT120I GGNCC 2 cut(s) 2515, 3405
BmiI GGNNCC 6 cut(s) 1360, 1881, 2066, 2719, 3333, 3406
BmrFI CCNGG 5 cut(s) 1539, 1793, 2401, 3161, 3409
BmrI ACTGGG 1 cut(s) 1345
BmuI ACTGGG 1 cut(s) 1345
BpiI GAAGAC 3 cut(s) 247, 3081, 3351
BplI GAGNNNNNCTC 4 cut(s) 90, 122, 1974, 2006
Bpu10I CCTNAGC 1 cut(s) 565
Bpu14I TTCGAA 1 cut(s) 1658
BpuEI CTTGAG 6 cut(s) 449, 1437, 1804, 2358, 2898, 3661
BsaHI GRCGYC 1 cut(s) 709
BsaI GGTCTC 4 cut(s) 93, 386, 968, 1451
BsaJI CCNNGG 2 cut(s) 558, 978
BsaXI ACNNNNNCTCC 8 cut(s) 24, 54, 177, 207, 687, 717, 741, 771
Bse3DI GCAATG 5 cut(s) 1088, 2424, 2649, 3438, 3627
BseBI CCWGG 5 cut(s) 1539, 1793, 2401, 3161, 3409
BseDI CCNNGG 2 cut(s) 558, 978
BseMI GCAATG 5 cut(s) 1088, 2424, 2649, 3438, 3627
BseRI GAGGAG 3 cut(s) 692, 1187, 2361
BseXI GCAGC 8 cut(s) 76, 281, 647, 1396, 1694, 1782, 1918, 3707
BseYI CCCAGC 1 cut(s) 1967
BsgI GTGCAG 1 cut(s) 2789
Bsh1236I CGCG 2 cut(s) 70, 420
BshFI GGCC 4 cut(s) 149, 1542, 2516, 3407
BsiHKAI GWGCWC 5 cut(s) 542, 683, 2853, 3196, 3355
BslFI GGGAC 4 cut(s) 1333, 1594, 3259, 3610
BsmBI CGTCTC 2 cut(s) 716, 3641
BsmFI GGGAC 4 cut(s) 1333, 1594, 3259, 3610
BsmI GAATGC 2 cut(s) 523, 1626
BsnI GGCC 4 cut(s) 149, 1542, 2516, 3407
Bso31I GGTCTC 4 cut(s) 93, 386, 968, 1451
Bsp119I TTCGAA 1 cut(s) 1658
Bsp1286I GDGCHC 6 cut(s) 542, 566, 683, 2853, 3196, 3355
BspACI CCGC 4 cut(s) 74, 418, 525, 2388
BspANI GGCC 4 cut(s) 149, 1542, 2516, 3407
BspFNI CGCG 2 cut(s) 70, 420
BspLI GGNNCC 6 cut(s) 1360, 1881, 2066, 2719, 3333, 3406
BspMAI CTGCAG 2 cut(s) 366, 3170
BspMI ACCTGC 1 cut(s) 369
BspPI GGATC 7 cut(s) 470, 535, 2257, 2408, 2703, 2712, 2725
BspQI GCTCTTC 2 cut(s) 530, 2049
BspT104I TTCGAA 1 cut(s) 1658
BspTI CTTAAG 1 cut(s) 1925
BspTNI GGTCTC 4 cut(s) 93, 386, 968, 1451
BsrBI CCGCTC 1 cut(s) 74
BsrDI GCAATG 5 cut(s) 1088, 2424, 2649, 3438, 3627
BssECI CCNNGG 2 cut(s) 558, 978
BssNAI GTATAC 2 cut(s) 941, 3239
BssNI GRCGYC 1 cut(s) 709
BssT1I CCWWGG 1 cut(s) 558
Bst1107I GTATAC 2 cut(s) 941, 3239
Bst2UI CCWGG 5 cut(s) 1539, 1793, 2401, 3161, 3409
Bst4CI ACNGT 9 cut(s) 235, 1099, 1430, 1553, 1585, 1616, 2141, 3350, 3368
Bst6I CTCTTC 4 cut(s) 530, 1956, 2049, 2644
BstACI GRCGYC 1 cut(s) 709
BstAFI CTTAAG 1 cut(s) 1925
BstAPI GCANNNNNTGC 2 cut(s) 291, 965
BstBI TTCGAA 1 cut(s) 1658
BstC8I GCNNGC 5 cut(s) 72, 523, 542, 1382, 3492
BstFNI CGCG 2 cut(s) 70, 420
BstHHI GCGC 1 cut(s) 3528
BstMWI GCNNNNNNNGC 8 cut(s) 291, 370, 965, 1779, 2924, 3487, 3525, 3755
BstNI CCWGG 5 cut(s) 1539, 1793, 2401, 3161, 3409
BstNSI RCATGY 6 cut(s) 1120, 1261, 1726, 2353, 2493, 3494
BstSCI CCNGG 5 cut(s) 1537, 1791, 2399, 3159, 3407
BstSFI CTRYAG 2 cut(s) 362, 3166
BstUI CGCG 2 cut(s) 70, 420
BstV1I GCAGC 8 cut(s) 76, 281, 647, 1396, 1694, 1782, 1918, 3707
BstV2I GAAGAC 3 cut(s) 247, 3081, 3351
BstX2I RGATCY 5 cut(s) 1902, 2105, 2160, 2695, 2717
BstYI RGATCY 5 cut(s) 1902, 2105, 2160, 2695, 2717
BstZ17I GTATAC 2 cut(s) 941, 3239
BsuI GTATCC 1 cut(s) 2451
BsuRI GGCC 4 cut(s) 149, 1542, 2516, 3407
BtgZI GCGATG 1 cut(s) 435
BtrI CACGTC 1 cut(s) 3079
BveI ACCTGC 1 cut(s) 369
Cac8I GCNNGC 5 cut(s) 72, 523, 542, 1382, 3492
CfoI GCGC 1 cut(s) 3528
Cfr13I GGNCC 2 cut(s) 2515, 3405
Csp6I GTAC 6 cut(s) 758, 1250, 1409, 1567, 2366, 2410
CspCI CAANNNNNGTGG 2 cut(s) 2632, 2667
CviQI GTAC 6 cut(s) 758, 1250, 1409, 1567, 2366, 2410
DraI TTTAAA 1 cut(s) 3019
EaeI YGGCCR 1 cut(s) 1540
Eam1104I CTCTTC 4 cut(s) 530, 1956, 2049, 2644
EarI CTCTTC 4 cut(s) 530, 1956, 2049, 2644
EciI GGCGGA 1 cut(s) 540
Ecl136II GAGCTC 2 cut(s) 540, 2851
Eco130I CCWWGG 1 cut(s) 558
Eco24I GRGCYC 3 cut(s) 542, 566, 2853
Eco31I GGTCTC 4 cut(s) 93, 386, 968, 1451
Eco32I GATATC 3 cut(s) 2394, 2559, 3502
Eco53kI GAGCTC 2 cut(s) 540, 2851
Eco57I CTGAAG 6 cut(s) 149, 501, 518, 1356, 1977, 2322
EcoICRI GAGCTC 2 cut(s) 540, 2851
EcoRI GAATTC 1 cut(s) 2266
EcoRII CCWGG 5 cut(s) 1537, 1791, 2399, 3159, 3407
EcoRV GATATC 3 cut(s) 2394, 2559, 3502
EcoT14I CCWWGG 1 cut(s) 558
EcoT22I ATGCAT 5 cut(s) 1507, 1724, 2980, 3440, 3555
EcoT38I GRGCYC 3 cut(s) 542, 566, 2853
ErhI CCWWGG 1 cut(s) 558
Esp3I CGTCTC 2 cut(s) 716, 3641
FaqI GGGAC 4 cut(s) 1333, 1594, 3259, 3610
FauI CCCGC 1 cut(s) 2381
FauNDI CATATG 2 cut(s) 2443, 2980
FbaI TGATCA 2 cut(s) 1278, 2983
FblI GTMKAC 4 cut(s) 493, 940, 2686, 3238
Fnu4HI GCNGC 8 cut(s) 90, 295, 636, 1385, 1683, 1771, 1932, 3696
FriOI GRGCYC 3 cut(s) 542, 566, 2853
Fsp4HI GCNGC 8 cut(s) 90, 295, 636, 1385, 1683, 1771, 1932, 3696
GlaI GCGC 1 cut(s) 3527
GluI GCNGC 8 cut(s) 90, 295, 636, 1385, 1683, 1771, 1932, 3696
GsaI CCCAGC 1 cut(s) 1971
HaeIII GGCC 4 cut(s) 149, 1542, 2516, 3407
HhaI GCGC 1 cut(s) 3528
Hin1I GRCGYC 1 cut(s) 709
Hin6I GCGC 1 cut(s) 3526
HinP1I GCGC 1 cut(s) 3526
HincII GTYRAC 2 cut(s) 1324, 1800
HindII GTYRAC 2 cut(s) 1324, 1800
HindIII AAGCTT 2 cut(s) 2916, 3747
HpaI GTTAAC 1 cut(s) 1800
HphI GGTGA 5 cut(s) 997, 2147, 2578, 3083, 3804
Hpy99I CGWCG 2 cut(s) 711, 864
HpyCH4III ACNGT 9 cut(s) 235, 1099, 1430, 1553, 1585, 1616, 2141, 3350, 3368
HpyCH4IV ACGT 5 cut(s) 709, 2538, 2683, 3078, 3634
HpyF10VI GCNNNNNNNGC 8 cut(s) 291, 370, 965, 1779, 2924, 3487, 3525, 3755
HpySE526I ACGT 5 cut(s) 709, 2538, 2683, 3078, 3634
Hsp92I GRCGYC 1 cut(s) 709
HspAI GCGC 1 cut(s) 3526
Ksp22I TGATCA 2 cut(s) 1278, 2983
KspAI GTTAAC 1 cut(s) 1800
LguI GCTCTTC 2 cut(s) 530, 2049
LmnI GCTCC 7 cut(s) 370, 511, 1364, 1849, 1995, 2070, 2360
Lsp1109I GCAGC 8 cut(s) 76, 281, 647, 1396, 1694, 1782, 1918, 3707
MaeII ACGT 5 cut(s) 709, 2538, 2683, 3078, 3634
MbiI CCGCTC 1 cut(s) 74
MfeI CAATTG 1 cut(s) 1737
MflI RGATCY 5 cut(s) 1902, 2105, 2160, 2695, 2717
MhlI GDGCHC 6 cut(s) 542, 566, 683, 2853, 3196, 3355
MlsI TGGCCA 1 cut(s) 1542
MluNI TGGCCA 1 cut(s) 1542
MlyI GAGTC 7 cut(s) 199, 1098, 1355, 1784, 2524, 2592, 3215
MmeI TCCRAC 7 cut(s) 217, 348, 429, 730, 1081, 1219, 1827
Mox20I TGGCCA 1 cut(s) 1542
Mph1103I ATGCAT 5 cut(s) 1507, 1724, 2980, 3440, 3555
MscI TGGCCA 1 cut(s) 1542
MslI CAYNNNNRTG 3 cut(s) 686, 1998, 3558
Msp20I TGGCCA 1 cut(s) 1542
MspCI CTTAAG 1 cut(s) 1925
MspR9I CCNGG 5 cut(s) 1539, 1793, 2401, 3161, 3409
MunI CAATTG 1 cut(s) 1737
Mva1269I GAATGC 2 cut(s) 523, 1626
MvaI CCWGG 5 cut(s) 1539, 1793, 2401, 3161, 3409
MvnI CGCG 2 cut(s) 70, 420
MwoI GCNNNNNNNGC 8 cut(s) 291, 370, 965, 1779, 2924, 3487, 3525, 3755
NdeI CATATG 2 cut(s) 2443, 2980
NlaIV GGNNCC 6 cut(s) 1360, 1881, 2066, 2719, 3333, 3406
NmuCI GTSAC 4 cut(s) 1424, 2135, 2141, 2584
NsiI ATGCAT 5 cut(s) 1507, 1724, 2980, 3440, 3555
NspI RCATGY 6 cut(s) 1120, 1261, 1726, 2353, 2493, 3494
NspV TTCGAA 1 cut(s) 1658
PaeI GCATGC 1 cut(s) 3494
PciI ACATGT 1 cut(s) 2349
PciSI GCTCTTC 2 cut(s) 530, 2049
PcsI WCGNNNNNNNCGW 1 cut(s) 3075
PctI GAATGC 2 cut(s) 523, 1626
PflFI GACNNNGTC 1 cut(s) 1007
PflMI CCANNNNNTGG 2 cut(s) 746, 2540
PkrI GCNGC 8 cut(s) 91, 296, 637, 1386, 1684, 1772, 1933, 3697
PleI GAGTC 7 cut(s) 198, 1098, 1354, 1783, 2524, 2591, 3215
PpsI GAGTC 7 cut(s) 198, 1098, 1354, 1783, 2524, 2591, 3215
PscI ACATGT 1 cut(s) 2349
Psp124BI GAGCTC 2 cut(s) 542, 2853
Psp1406I AACGTT 1 cut(s) 2538
Psp6I CCWGG 5 cut(s) 1537, 1791, 2399, 3159, 3407
PspFI CCCAGC 1 cut(s) 1967
PspGI CCWGG 5 cut(s) 1537, 1791, 2399, 3159, 3407
PspN4I GGNNCC 6 cut(s) 1360, 1881, 2066, 2719, 3333, 3406
PspPI GGNCC 2 cut(s) 2515, 3405
PstI CTGCAG 2 cut(s) 366, 3170
PsuI RGATCY 5 cut(s) 1902, 2105, 2160, 2695, 2717
PsyI GACNNNGTC 1 cut(s) 1007
RsaI GTAC 6 cut(s) 759, 1251, 1410, 1568, 2367, 2411
RsaNI GTAC 6 cut(s) 758, 1250, 1409, 1567, 2366, 2410
RseI CAYNNNNRTG 3 cut(s) 686, 1998, 3558
SacI GAGCTC 2 cut(s) 542, 2853
SapI GCTCTTC 2 cut(s) 530, 2049
SatI GCNGC 8 cut(s) 90, 295, 636, 1385, 1683, 1771, 1932, 3696
Sau96I GGNCC 2 cut(s) 2515, 3405
ScaI AGTACT 1 cut(s) 1251
SchI GAGTC 7 cut(s) 199, 1098, 1355, 1784, 2524, 2592, 3215
ScrFI CCNGG 5 cut(s) 1539, 1793, 2401, 3161, 3409
SduI GDGCHC 6 cut(s) 542, 566, 683, 2853, 3196, 3355
SfcI CTRYAG 2 cut(s) 362, 3166
SfuI TTCGAA 1 cut(s) 1658
SmiI ATTTAAAT 1 cut(s) 3019
SmiMI CAYNNNNRTG 3 cut(s) 686, 1998, 3558
SmlI CTYRAG 7 cut(s) 428, 1452, 1819, 1925, 2373, 2913, 3640
SmoI CTYRAG 7 cut(s) 428, 1452, 1819, 1925, 2373, 2913, 3640
SpeI ACTAGT 1 cut(s) 625
SphI GCATGC 1 cut(s) 3494
SsiI CCGC 4 cut(s) 74, 418, 525, 2388
SspI AATATT 4 cut(s) 997, 2208, 2842, 3789
SstI GAGCTC 2 cut(s) 542, 2853
StyD4I CCNGG 5 cut(s) 1537, 1791, 2399, 3159, 3407
StyI CCWWGG 1 cut(s) 558
SwaI ATTTAAAT 1 cut(s) 3019
TaaI ACNGT 9 cut(s) 235, 1099, 1430, 1553, 1585, 1616, 2141, 3350, 3368
TaiI ACGT 5 cut(s) 712, 2541, 2686, 3081, 3637
TaqI TCGA 5 cut(s) 1107, 1170, 1658, 2149, 3279
TatI WGTACW 3 cut(s) 1249, 1408, 1566
TseFI GTSAC 4 cut(s) 1424, 2135, 2141, 2584
TseI GCWGC 8 cut(s) 89, 294, 635, 1384, 1682, 1770, 1931, 3695
Tsp45I GTSAC 4 cut(s) 1424, 2135, 2141, 2584
TspGWI ACGGA 2 cut(s) 1351, 2564
Tth111I GACNNNGTC 1 cut(s) 1007
Van91I CCANNNNNTGG 2 cut(s) 746, 2540
Vha464I CTTAAG 1 cut(s) 1925
XbaI TCTAGA 2 cut(s) 1492, 3217
XceI RCATGY 6 cut(s) 1120, 1261, 1726, 2353, 2493, 3494
XmiI GTMKAC 4 cut(s) 493, 940, 2686, 3238
ZraI GACGTC 1 cut(s) 710
ZrmI AGTACT 1 cut(s) 1251
Zsp2I ATGCAT 5 cut(s) 1507, 1724, 2980, 3440, 3555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.