Rorug02G0122000

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0002237
Physical Location & Seq
Forward (+)
10523732 .. 10525556
1825 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0122000.1

Sequence Viewer

Length: 867 bp
ATGGCGGAACCTGTCGTAGACGCGCGGTACCTGAAAGAAATCGAGAAGGCTCGTCGGGACCTCCGCGCTCTCATCTCCAACCGCAACTGCGCTCCCATCATGCTTCGTCTGGCGTGGCACGATGCTGGAACTTACAACGCTGAAACGAAGACTGGAGGGGCGACTGGCTCCATCAGGAATGAGCATGAGTTGGGCCATGGAGCTAATAGTGGTTTGAAAATTGCTGTTAATTTTTGTGAGGAAGTGAAAGCCAAACATCCGAAAATCACATATGCTGACCTTTACCAGCTTGCTGGGGTTGTTGCAGTCGAGGTCACCGGAGGCCCTACTATTGAGTTTGTTCCCGGTAGGAAGGATTCATTGGAATCTCCTGAAGAAGGAAGGCTTCCGGATGCCAAAAAAGGTGCACCACATTTGAAGGACATCTTTTACCGCATGGGTCTGTCTGATAAGGACATTGTGGCACTGTCTGGAGCCCATACATTGGGTAGAGCACATCCAGAAAGGTCAGGCTTTGATGGCCCTTGGACAAAAGAGCCTCTTAAGTTTGACAATTCATACTTTGTGGAGCTACTGAAAGGAGAATCAGAGGGGCTGTTGAAACTACCCACAGACAAGGCTTTATTGGATGATCCTGCGTTCCGCCCCTACGTTGAACTTTATGCAAAGGATGAGGATGCATTTTTCAGAGACTATGCAGCATCACAGAAGAAACTATCAGAGCTAGGCTTCACTCCACCTCCATCTAGTCTCAAGGAAGCTGTAAACACCAGTGTCGTAGTGGCACAAAGTGCTGTGGGAGTTGCAGTTGCAGCTGCGGTGGTGATTCTGGGTTACTTATACGAAGTTCATAAAAGAGTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KAP U-box

Protein Analysis

288

Amino Acids

31.48

Weight (kDa)

6.47

Isoelectric Point (pI)

35.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 18 - 224 5.2e-49 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 27
AccB1I GGYRCC 1 cut(s) 27
AccB7I CCANNNNNTGG 1 cut(s) 484
AccI GTMKAC 1 cut(s) 18
AccII CGCG 3 cut(s) 23, 25, 66
AccIII TCCGGA 1 cut(s) 388
AciI CCGC 7 cut(s) 5, 25, 64, 82, 433, 643, 818
AclWI GGATC 1 cut(s) 626
AcuI CTGAAG 1 cut(s) 393
AdeI CACNNNGTG 1 cut(s) 791
AfaI GTAC 1 cut(s) 29
AfiI CCNNNNNNNGG 2 cut(s) 377, 484
AflII CTTAAG 1 cut(s) 542
AgsI TTSAA 4 cut(s) 217, 418, 601, 656
AjuI GAANNNNNNNTTGG 2 cut(s) 344, 376
AluBI AGCT 6 cut(s) 203, 289, 571, 724, 761, 815
AluI AGCT 6 cut(s) 203, 289, 571, 724, 761, 815
Alw21I GWGCWC 2 cut(s) 409, 496
Alw26I GTCTC 2 cut(s) 684, 755
Alw44I GTGCAC 1 cut(s) 405
AlwI GGATC 1 cut(s) 626
Aor13HI TCCGGA 1 cut(s) 388
AoxI GGCC 3 cut(s) 193, 322, 520
ApaLI GTGCAC 1 cut(s) 405
ApeKI GCWGC 3 cut(s) 698, 812, 815
Asp718I GGTACC 1 cut(s) 27
AspLEI GCGC 3 cut(s) 25, 68, 92
AspS9I GGNCC 4 cut(s) 58, 193, 323, 521
AsuC2I CCSGG 1 cut(s) 345
AsuHPI GGTGA 2 cut(s) 307, 835
AvaII GGWCC 1 cut(s) 58
BaeGI GKGCMC 1 cut(s) 409
BanI GGYRCC 1 cut(s) 27
BanII GRGCYC 1 cut(s) 478
BbsI GAAGAC 1 cut(s) 155
Bbv12I GWGCWC 2 cut(s) 409, 496
BbvI GCAGC 3 cut(s) 710, 802, 824
BccI CCATC 4 cut(s) 104, 179, 512, 751
BcnI CCSGG 1 cut(s) 345
BcoDI GTCTC 2 cut(s) 684, 755
BfaI CTAG 2 cut(s) 725, 747
BfrI CTTAAG 1 cut(s) 542
BisI GCNGC 3 cut(s) 699, 813, 816
BlsI GCNGC 3 cut(s) 700, 814, 817
Bme1390I CCNGG 1 cut(s) 345
Bme18I GGWCC 1 cut(s) 58
BmgT120I GGNCC 4 cut(s) 58, 193, 323, 521
BmiI GGNNCC 5 cut(s) 9, 29, 59, 169, 475
BmrFI CCNGG 1 cut(s) 345
BmsI GCATC 4 cut(s) 112, 382, 667, 710
BpiI GAAGAC 1 cut(s) 155
BpmI CTGGAG 2 cut(s) 174, 492
BpuEI CTTGAG 1 cut(s) 737
BpuMI CCSGG 1 cut(s) 345
BsaJI CCNNGG 2 cut(s) 196, 524
BsaWI WCCGGW 2 cut(s) 317, 388
BsaXI ACNNNNNCTCC 4 cut(s) 724, 754, 792, 822
Bsc4I CCNNNNNNNGG 2 cut(s) 377, 484
Bse1I ACTGG 3 cut(s) 157, 169, 771
BseAI TCCGGA 1 cut(s) 388
BseDI CCNNGG 2 cut(s) 196, 524
BseGI GGATG 6 cut(s) 256, 397, 496, 634, 676, 682
BseLI CCNNNNNNNGG 2 cut(s) 377, 484
BseNI ACTGG 3 cut(s) 157, 169, 771
BseSI GKGCMC 1 cut(s) 409
BseXI GCAGC 3 cut(s) 710, 802, 824
BseYI CCCAGC 1 cut(s) 293
Bsh1236I CGCG 3 cut(s) 23, 25, 66
BshFI GGCC 3 cut(s) 195, 324, 522
BshNI GGYRCC 1 cut(s) 27
BsiHKAI GWGCWC 2 cut(s) 409, 496
BsiSI CCGG 3 cut(s) 318, 345, 389
BslFI GGGAC 1 cut(s) 71
BslI CCNNNNNNNGG 2 cut(s) 377, 484
BsmAI GTCTC 2 cut(s) 684, 755
BsmFI GGGAC 1 cut(s) 71
BsnI GGCC 3 cut(s) 195, 324, 522
Bsp1286I GDGCHC 3 cut(s) 409, 478, 496
Bsp13I TCCGGA 1 cut(s) 388
Bsp143I GATC 1 cut(s) 631
Bsp19I CCATGG 1 cut(s) 196
BspACI CCGC 7 cut(s) 5, 25, 64, 82, 433, 643, 818
BspANI GGCC 3 cut(s) 195, 324, 522
BspEI TCCGGA 1 cut(s) 388
BspFNI CGCG 3 cut(s) 23, 25, 66
BspLI GGNNCC 5 cut(s) 9, 29, 59, 169, 475
BspPI GGATC 1 cut(s) 626
BspT107I GGYRCC 1 cut(s) 27
BspTI CTTAAG 1 cut(s) 542
BsrI ACTGG 3 cut(s) 157, 169, 771
BssECI CCNNGG 2 cut(s) 196, 524
BssMI GATC 1 cut(s) 631
BssT1I CCWWGG 2 cut(s) 196, 524
Bst4CI ACNGT 1 cut(s) 468
BstAFI CTTAAG 1 cut(s) 542
BstAPI GCANNNNNTGC 1 cut(s) 791
BstC8I GCNNGC 1 cut(s) 291
BstDSI CCRYGG 1 cut(s) 196
BstEII GGTNACC 1 cut(s) 313
BstENI CCTNNNNNAGG 1 cut(s) 375
BstF5I GGATG 6 cut(s) 256, 397, 496, 634, 676, 682
BstFNI CGCG 3 cut(s) 23, 25, 66
BstHHI GCGC 3 cut(s) 25, 68, 92
BstKTI GATC 1 cut(s) 634
BstMAI GTCTC 2 cut(s) 684, 755
BstMBI GATC 1 cut(s) 631
BstMWI GCNNNNNNNGC 3 cut(s) 519, 791, 812
BstPI GGTNACC 1 cut(s) 313
BstSCI CCNGG 1 cut(s) 343
BstSLI GKGCMC 1 cut(s) 409
BstUI CGCG 3 cut(s) 23, 25, 66
BstV1I GCAGC 3 cut(s) 710, 802, 824
BstV2I GAAGAC 1 cut(s) 155
BstXI CCANNNNNNTGG 1 cut(s) 293
BsuRI GGCC 3 cut(s) 195, 324, 522
BtgI CCRYGG 1 cut(s) 196
BtsCI GGATG 6 cut(s) 256, 397, 496, 634, 676, 682
BtsIMutI CAGTG 2 cut(s) 464, 778
Cac8I GCNNGC 1 cut(s) 291
CfoI GCGC 3 cut(s) 25, 68, 92
Cfr13I GGNCC 4 cut(s) 58, 193, 323, 521
CseI GACGC 1 cut(s) 29
Csp6I GTAC 1 cut(s) 28
CviAII CATG 4 cut(s) 100, 185, 197, 436
CviQI GTAC 1 cut(s) 28
DpnI GATC 1 cut(s) 633
DpnII GATC 1 cut(s) 631
DraIII CACNNNGTG 1 cut(s) 791
EciI GGCGGA 2 cut(s) 20, 632
Eco130I CCWWGG 2 cut(s) 196, 524
Eco24I GRGCYC 1 cut(s) 478
Eco47I GGWCC 1 cut(s) 58
Eco57I CTGAAG 1 cut(s) 393
Eco91I GGTNACC 1 cut(s) 313
EcoNI CCTNNNNNAGG 1 cut(s) 375
EcoO109I RGGNCCY 2 cut(s) 58, 323
EcoO65I GGTNACC 1 cut(s) 313
EcoT14I CCWWGG 2 cut(s) 196, 524
EcoT22I ATGCAT 1 cut(s) 682
EcoT38I GRGCYC 1 cut(s) 478
ErhI CCWWGG 2 cut(s) 196, 524
FaeI CATG 4 cut(s) 103, 188, 200, 439
FalI AAGNNNNNCTT 6 cut(s) 369, 401, 410, 442, 525, 557
FaqI GGGAC 1 cut(s) 71
FatI CATG 4 cut(s) 99, 184, 196, 435
FauNDI CATATG 1 cut(s) 271
FblI GTMKAC 1 cut(s) 18
Fnu4HI GCNGC 3 cut(s) 699, 813, 816
FokI GGATG 6 cut(s) 243, 404, 483, 641, 683, 689
FriOI GRGCYC 1 cut(s) 478
Fsp4HI GCNGC 3 cut(s) 699, 813, 816
FspBI CTAG 2 cut(s) 725, 747
GlaI GCGC 3 cut(s) 24, 67, 91
GluI GCNGC 3 cut(s) 699, 813, 816
GsaI CCCAGC 1 cut(s) 297
GsuI CTGGAG 2 cut(s) 174, 492
HaeIII GGCC 3 cut(s) 195, 324, 522
HapII CCGG 3 cut(s) 318, 345, 389
HgaI GACGC 1 cut(s) 29
HhaI GCGC 3 cut(s) 25, 68, 92
Hin1II CATG 4 cut(s) 103, 188, 200, 439
Hin6I GCGC 3 cut(s) 23, 66, 90
HinP1I GCGC 3 cut(s) 23, 66, 90
HinfI GANTC 5 cut(s) 356, 365, 584, 826, 858
HpaII CCGG 3 cut(s) 318, 345, 389
HphI GGTGA 2 cut(s) 307, 835
Hpy166II GTNNAC 3 cut(s) 19, 407, 766
Hpy188I TCNGA 5 cut(s) 261, 448, 589, 689, 721
Hpy188III TCNNGA 7 cut(s) 43, 56, 175, 371, 389, 471, 500
Hpy8I GTNNAC 3 cut(s) 19, 407, 766
Hpy99I CGWCG 1 cut(s) 57
HpyAV CCTTC 5 cut(s) 40, 346, 371, 375, 412
HpyCH4III ACNGT 1 cut(s) 468
HpyCH4IV ACGT 1 cut(s) 651
HpyCH4V TGCA 7 cut(s) 305, 407, 665, 680, 698, 806, 812
HpyF10VI GCNNNNNNNGC 3 cut(s) 519, 791, 812
HpySE526I ACGT 1 cut(s) 651
Hsp92II CATG 4 cut(s) 103, 188, 200, 439
HspAI GCGC 3 cut(s) 23, 66, 90
Kpn2I TCCGGA 1 cut(s) 388
KpnI GGTACC 1 cut(s) 31
Kzo9I GATC 1 cut(s) 631
LmnI GCTCC 5 cut(s) 97, 173, 200, 473, 568
Lsp1109I GCAGC 3 cut(s) 710, 802, 824
LweI GCATC 4 cut(s) 112, 382, 667, 710
MaeI CTAG 2 cut(s) 725, 747
MaeII ACGT 1 cut(s) 651
MaeIII GTNAC 2 cut(s) 313, 833
MalI GATC 1 cut(s) 633
MboI GATC 1 cut(s) 631
MboII GAAGA 3 cut(s) 160, 386, 721
MhlI GDGCHC 3 cut(s) 409, 478, 496
MluCI AATT 3 cut(s) 219, 229, 553
MlyI GAGTC 1 cut(s) 867
MmeI TCCRAC 1 cut(s) 102
MnlI CCTC 9 cut(s) 71, 149, 232, 304, 314, 549, 583, 667, 750
Mph1103I ATGCAT 1 cut(s) 682
MroI TCCGGA 1 cut(s) 388
MseI TTAA 2 cut(s) 228, 543
MspA1I CMGCKG 1 cut(s) 815
MspCI CTTAAG 1 cut(s) 542
MspI CCGG 3 cut(s) 318, 345, 389
MspR9I CCNGG 1 cut(s) 345
MvnI CGCG 3 cut(s) 23, 25, 66
MwoI GCNNNNNNNGC 3 cut(s) 519, 791, 812
NciI CCSGG 1 cut(s) 345
NcoI CCATGG 1 cut(s) 196
NdeI CATATG 1 cut(s) 271
NdeII GATC 1 cut(s) 631
NlaIII CATG 4 cut(s) 103, 188, 200, 439
NlaIV GGNNCC 5 cut(s) 9, 29, 59, 169, 475
NmuCI GTSAC 1 cut(s) 313
NsiI ATGCAT 1 cut(s) 682
PfeI GAWTC 4 cut(s) 356, 365, 584, 826
PflMI CCANNNNNTGG 1 cut(s) 484
PkrI GCNGC 3 cut(s) 700, 814, 817
PleI GAGTC 1 cut(s) 866
PpsI GAGTC 1 cut(s) 866
PpuMI RGGWCCY 1 cut(s) 58
Psp5II RGGWCCY 1 cut(s) 58
PspEI GGTNACC 1 cut(s) 313
PspFI CCCAGC 1 cut(s) 293
PspN4I GGNNCC 5 cut(s) 9, 29, 59, 169, 475
PspPI GGNCC 4 cut(s) 58, 193, 323, 521
PspPPI RGGWCCY 1 cut(s) 58
PvuII CAGCTG 1 cut(s) 815
RsaI GTAC 1 cut(s) 29
RsaNI GTAC 1 cut(s) 28
SaqAI TTAA 2 cut(s) 228, 543
SatI GCNGC 3 cut(s) 699, 813, 816
Sau3AI GATC 1 cut(s) 631
Sau96I GGNCC 4 cut(s) 58, 193, 323, 521
SchI GAGTC 1 cut(s) 867
ScrFI CCNGG 1 cut(s) 345
SduI GDGCHC 3 cut(s) 409, 478, 496
SfaNI GCATC 4 cut(s) 112, 382, 667, 710
SinI GGWCC 1 cut(s) 58
SmlI CTYRAG 2 cut(s) 542, 752
SmoI CTYRAG 2 cut(s) 542, 752
Sse9I AATT 3 cut(s) 219, 229, 553
SsiI CCGC 7 cut(s) 5, 25, 64, 82, 433, 643, 818
SspMI CTAG 2 cut(s) 725, 747
StyD4I CCNGG 1 cut(s) 343
StyI CCWWGG 2 cut(s) 196, 524
TaaI ACNGT 1 cut(s) 468
TaiI ACGT 1 cut(s) 654
TaqI TCGA 2 cut(s) 42, 309
TasI AATT 3 cut(s) 219, 229, 553
TfiI GAWTC 4 cut(s) 356, 365, 584, 826
Tru1I TTAA 2 cut(s) 228, 543
Tru9I TTAA 2 cut(s) 228, 543
TscAI CASTG 2 cut(s) 471, 778
TseFI GTSAC 1 cut(s) 313
TseI GCWGC 3 cut(s) 698, 812, 815
Tsp45I GTSAC 1 cut(s) 313
TspDTI ATGAA 3 cut(s) 348, 546, 839
TspRI CASTG 2 cut(s) 471, 778
Van91I CCANNNNNTGG 1 cut(s) 484
Vha464I CTTAAG 1 cut(s) 542
VneI GTGCAC 1 cut(s) 405
VpaK11BI GGWCC 1 cut(s) 58
XagI CCTNNNNNAGG 1 cut(s) 375
XcmI CCANNNNNNNNNTGG 1 cut(s) 778
XmiI GTMKAC 1 cut(s) 18
XspI CTAG 2 cut(s) 725, 747
Zsp2I ATGCAT 1 cut(s) 682
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.