Rw2G013550

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0002237
Physical Location & Seq
Reverse (-)
14585740 .. 14589571
3832 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G013550.1

Sequence Viewer

Length: 2262 bp
ATGATATCAATATCGAATCAGCACAAACCTGATGAAATTGTTCACTGTTCAATGTGCATTCAACTTTGGAAGTTGGTGGAGAGAATTTCAAAGATATTTCCCGACATAGAAGCAGCAAGACCTCGTTGCTCAACGGGAATACAGGCTCTATGCATGTTGAATCGTGCAATTGAGAAAGCCAAGCAACTTGTTCAGTATTGCAGCGAGTCTAGCAAACTCTACCTGGCGTGTACAGGGGATGTGATAGTCTCAAGATGTCAAAAATCAAGGAACTTGTTGGAGCAAAGTTTGTGCCAGATTCAGAGTTGGGTTCCAGTGATTTTGGTGGCAGAGATCTCTACAATAGTTGATGATCTTAAGGCTGCAACATTTAGACTGCATCCATCTGAAGAAGAGGCTGGGAAGGTCATCCGAGAATTGCTCCACCAAGATGCTTCTGCATCAAATTCAATAGAAGATTCTGAACTCAAGGCTCTTCAACTTGCATCTTTGAGGCTCCATATTACCACATCGGAGGAAGTCTTAATAGAGAAAAGATCTATTAAGAACTTGCTACAAAAAGTTGGTGACAGTGACCCTCGAAAAAAGCAGATCTTGACATATTTCTTGTATCTATTGAAGAAGTATGGAAACTTAATATTGGTAAAGCAAACAGAGAGTGCCCCTGTGTGGCAACAAGGATCACTTGCTTCTGAGAATTCTGGCAACGACTTTGGATATAATCAGTGTGCTGAAGTTGAACCTCACACTGGTTATGGGGAATATGAGGCACAAAGTAACATGTTGCTTGGAGCTGTACCTCCTCAAGAATTTTTGTGCGGGATATCTTCCAGGTTGATGTACGATCCTGTTGTCATTGCTTCTGGACAAACATATGAAAGGATGTGGATACAGAAGTGGTTTAATGAGGGAAATGATACATGCCCAAAAACTAATACCAAACTGGCCCACCTTTCATTGAATCCAAACGTTGGCATGAAGGAGTTGATATCAAGATGGTGTACGAAGTCTGGAGTCACCATCCCCAACCCAAGTATGCAACCAGAAGCACTAGGCTCATGGGAAAACTCTTCCACTTCCATTGCTAGTTTAGGCAGTTCTATGAATGATCTACGTCTACAAATGGATCTCAGCACTATGTCATTTGGATCCTTAGATACCAGTTTCAATTCTAAGACAGAAGATGGCTCAAATCTGGTGCAGATTGATGATGATTCTCTGAAATACATATCTGCTAAAAAATGCGAGACAGATTTGGAATTTCTATATAATATTAGTGAGCTCAAATGGGATTCCCAACGCAAGGCTGTTGAAGATGTTAAAAACGATTTGAAACGCAATCCTCAAGCTTCTTATGCTATGTCCTCTAAGAATTGTGTTGAACCTCTCATCAAATTTTTGAGGGATGCATATGATCAGGATGATGTAGAGGCTCAGAGAGATGGATTTAAATTATTATCTACCTTTGTCAGCAAAAACAGATACGGGATATGTTATTTAGGTGAAGACGTGTATAGTCTGTTAGTATCTTCTCTTGATTCAGAGGTAAAGGAGGACGGTCTTGCTATAATGGAAATTCTGTCTGTCCACCAGGACTGCAGAGGTAACATCTCATCATCTGGTGCTCTTACATCCATTCTAAAACTTCTAGACTCTCAGAGCAGAAGTATACAAGGAAAAGCCCTAAAAATTTTGCACAATTTGTCCTTCGATAGAGATATTTGTTCAGAGATGGTATCTTTGGAGTGCATCCCAAAATTGGTTCCTTTCTTTGAAGACAGTGCTCTTACTGGAAACTGTATCGCTATATTGAAAAATCTAAGCGATTATGAAGGGGCCAGGATTTCTATTGCTGAAACTGATGGATGCATTGCTTCTGTTATTTATGTCCTAGACACTGGCAGTCAAGAGCATCAAGAGCATGCTGTTGATATCCTTCTTTCTTTATGCTCTCAGCGCATTGACTATTGTAACTTGGTCATGCATGAGGGTGTAATTCCTGCTCTTGTCCTCTCATCTACCAATGGGACAGAGAGAACGAGGATAAACGCAATGGAAGTTCTACGTCTCTTGAGAGATATTGATTATGACAATGAGCAAGAATCTGCTGAACCTGAGCCTGAGGCAGCTAGAGATGAGCTACCTAGAGAAAATGGAAATCACTGGAACGGGAAGAAAGCTTCCAAGGCATCAGGATTTTTCGCAAAGAAGTTATCAATATTCTCAAAACCGAGTTCTCTTTCACCGAAAAAGAAGACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KAP U-box

Protein Analysis

753

Amino Acids

83.98

Weight (kDa)

5.3

Isoelectric Point (pI)

44.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 268 - 336 1.3e-17 U-box domain
ARM_PUB PF25598 499 - 693 2.8e-12 Plant U-box protein ARM repeat region
KAP PF05804 503 - 638 1.2e-07 Kinesin-associated protein (KAP)
Arm_2 PF04826 506 - 604 1.1e-06 Armadillo-like
ARM_ARMC5 PF24768 527 - 688 8.1e-07 ARMC5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 971
AccI GTMKAC 2 cut(s) 1117, 1669
AciI CCGC 1 cut(s) 819
AclI AACGTT 1 cut(s) 969
AclWI GGATC 5 cut(s) 688, 839, 1134, 1143, 1156
AcsI RAATTY 8 cut(s) 84, 445, 697, 809, 1259, 1394, 1575, 1689
AcuI CTGAAG 2 cut(s) 408, 753
AfaI GTAC 4 cut(s) 232, 798, 842, 1003
AfiI CCNNNNNNNGG 5 cut(s) 669, 749, 971, 1303, 1759
AflII CTTAAG 1 cut(s) 356
AflIII ACRYGT 2 cut(s) 780, 1509
AjiI CACGTC 1 cut(s) 1510
AjnI CCWGG 4 cut(s) 222, 830, 1590, 1838
AluBI AGCT 6 cut(s) 794, 1282, 1349, 2129, 2140, 2180
AluI AGCT 6 cut(s) 794, 1282, 1349, 2129, 2140, 2180
Alw21I GWGCWC 3 cut(s) 1284, 1627, 1786
Alw26I GTCTC 3 cut(s) 253, 1241, 2072
AlwI GGATC 5 cut(s) 688, 839, 1134, 1143, 1156
AoxI GGCC 2 cut(s) 945, 1836
ApeKI GCWGC 4 cut(s) 113, 201, 362, 2126
ApoI RAATTY 8 cut(s) 84, 445, 697, 809, 1259, 1394, 1575, 1689
Asp700I GAANNNNTTC 1 cut(s) 39
AspLEI GCGC 1 cut(s) 1959
AspS9I GGNCC 2 cut(s) 946, 1836
AsuHPI GGTGA 4 cut(s) 578, 1009, 1514, 2235
AxyI CCTNAGG 1 cut(s) 2121
BaeGI GKGCMC 1 cut(s) 664
BaeI ACNNNNGTAYC 2 cut(s) 881, 914
BamHI GGATCC 1 cut(s) 1148
BanII GRGCYC 1 cut(s) 1284
BbsI GAAGAC 2 cut(s) 1512, 1782
Bbv12I GWGCWC 3 cut(s) 1284, 1627, 1786
BbvI GCAGC 4 cut(s) 125, 213, 349, 2138
BccI CCATC 7 cut(s) 391, 990, 1028, 1178, 1436, 1726, 1856
BciT130I CCWGG 4 cut(s) 224, 832, 1592, 1840
BciVI GTATCC 1 cut(s) 882
BclI TGATCA 1 cut(s) 1414
BcoDI GTCTC 3 cut(s) 253, 1241, 2072
BfaI CTAG 7 cut(s) 210, 1052, 1086, 1649, 1892, 2130, 2145
BfmI CTRYAG 1 cut(s) 1597
BfrI CTTAAG 1 cut(s) 356
BfuI GTATCC 1 cut(s) 882
BglII AGATCT 3 cut(s) 333, 536, 591
BisI GCNGC 4 cut(s) 114, 202, 363, 2127
BlsI GCNGC 4 cut(s) 115, 203, 364, 2128
Bme1390I CCNGG 4 cut(s) 224, 832, 1592, 1840
BmgBI CACGTC 1 cut(s) 1510
BmgT120I GGNCC 2 cut(s) 946, 1836
BmiI GGNNCC 5 cut(s) 312, 497, 1150, 1764, 1837
BmrFI CCNGG 4 cut(s) 224, 832, 1592, 1840
BmsI GCATC 9 cut(s) 388, 421, 449, 494, 1396, 1758, 1856, 1921, 2198
BpiI GAAGAC 2 cut(s) 1512, 1782
BplI GAGNNNNNCTC 2 cut(s) 405, 437
BpmI CTGGAG 1 cut(s) 1032
Bpu10I CCTNAGC 1 cut(s) 2115
BpuEI CTTGAG 5 cut(s) 235, 452, 789, 1329, 2092
BsaJI CCNNGG 1 cut(s) 2184
Bsc4I CCNNNNNNNGG 5 cut(s) 669, 749, 971, 1303, 1759
Bse1I ACTGG 7 cut(s) 314, 754, 948, 1161, 1795, 1903, 2168
Bse21I CCTNAGG 1 cut(s) 2121
Bse3DI GCAATG 4 cut(s) 855, 1080, 1869, 2058
BseBI CCWGG 4 cut(s) 224, 832, 1592, 1840
BseDI CCNNGG 1 cut(s) 2184
BseLI CCNNNNNNNGG 5 cut(s) 669, 749, 971, 1303, 1759
BseMI GCAATG 4 cut(s) 855, 1080, 1869, 2058
BseMII CTCAG 7 cut(s) 684, 1144, 1448, 1670, 1967, 2106, 2112
BseNI ACTGG 7 cut(s) 314, 754, 948, 1161, 1795, 1903, 2168
BseRI GAGGAG 1 cut(s) 792
BseSI GKGCMC 1 cut(s) 664
BseXI GCAGC 4 cut(s) 125, 213, 349, 2138
BseYI CCCAGC 1 cut(s) 398
BsgI GTGCAG 1 cut(s) 1220
BshFI GGCC 2 cut(s) 947, 1838
BsiHKAI GWGCWC 3 cut(s) 1284, 1627, 1786
BslFI GGGAC 1 cut(s) 2041
BslI CCNNNNNNNGG 5 cut(s) 669, 749, 971, 1303, 1759
BsmAI GTCTC 3 cut(s) 253, 1241, 2072
BsmBI CGTCTC 1 cut(s) 2072
BsmFI GGGAC 1 cut(s) 2041
BsmI GAATGC 1 cut(s) 57
BsnI GGCC 2 cut(s) 947, 1838
Bsp1286I GDGCHC 4 cut(s) 664, 1284, 1627, 1786
Bsp1407I TGTACA 1 cut(s) 230
BspACI CCGC 1 cut(s) 819
BspANI GGCC 2 cut(s) 947, 1838
BspCNI CTCAG 7 cut(s) 685, 1143, 1447, 1669, 1966, 2107, 2113
BspLI GGNNCC 5 cut(s) 312, 497, 1150, 1764, 1837
BspMAI CTGCAG 1 cut(s) 1601
BspPI GGATC 5 cut(s) 688, 839, 1134, 1143, 1156
BspQI GCTCTTC 1 cut(s) 480
BspTI CTTAAG 1 cut(s) 356
BsrDI GCAATG 4 cut(s) 855, 1080, 1869, 2058
BsrGI TGTACA 1 cut(s) 230
BsrI ACTGG 7 cut(s) 314, 754, 948, 1161, 1795, 1903, 2168
BssECI CCNNGG 1 cut(s) 2184
BssNAI GTATAC 1 cut(s) 1670
BssT1I CCWWGG 1 cut(s) 2184
Bst1107I GTATAC 1 cut(s) 1670
Bst2UI CCWGG 4 cut(s) 224, 832, 1592, 1840
Bst4CI ACNGT 5 cut(s) 47, 572, 1559, 1781, 1799
Bst6I CTCTTC 3 cut(s) 387, 480, 1075
BstAFI CTTAAG 1 cut(s) 356
BstAUI TGTACA 1 cut(s) 230
BstC8I GCNNGC 1 cut(s) 1923
BstHHI GCGC 1 cut(s) 1959
BstMAI GTCTC 3 cut(s) 253, 1241, 2072
BstMWI GCNNNNNNNGC 5 cut(s) 210, 1355, 1918, 1956, 2186
BstNI CCWGG 4 cut(s) 224, 832, 1592, 1840
BstNSI RCATGY 4 cut(s) 157, 784, 924, 1925
BstSCI CCNGG 4 cut(s) 222, 830, 1590, 1838
BstSFI CTRYAG 1 cut(s) 1597
BstSLI GKGCMC 1 cut(s) 664
BstV1I GCAGC 4 cut(s) 125, 213, 349, 2138
BstV2I GAAGAC 2 cut(s) 1512, 1782
BstX2I RGATCY 5 cut(s) 333, 536, 591, 1126, 1148
BstYI RGATCY 5 cut(s) 333, 536, 591, 1126, 1148
BstZ17I GTATAC 1 cut(s) 1670
Bsu36I CCTNAGG 1 cut(s) 2121
BsuI GTATCC 1 cut(s) 882
BsuRI GGCC 2 cut(s) 947, 1838
BtrI CACGTC 1 cut(s) 1510
BtsIMutI CAGTG 8 cut(s) 43, 321, 577, 731, 747, 1786, 1896, 2161
Cac8I GCNNGC 1 cut(s) 1923
CfoI GCGC 1 cut(s) 1959
Cfr13I GGNCC 2 cut(s) 946, 1836
Csp6I GTAC 4 cut(s) 231, 797, 841, 1002
CspCI CAANNNNNGTGG 2 cut(s) 1063, 1098
CviAII CATG 9 cut(s) 154, 781, 921, 976, 1059, 1922, 1981, 1985, 2259
CviQI GTAC 4 cut(s) 231, 797, 841, 1002
DraI TTTAAA 1 cut(s) 1450
Eam1104I CTCTTC 3 cut(s) 387, 480, 1075
EarI CTCTTC 3 cut(s) 387, 480, 1075
Ecl136II GAGCTC 1 cut(s) 1282
Eco130I CCWWGG 1 cut(s) 2184
Eco24I GRGCYC 1 cut(s) 1284
Eco32I GATATC 4 cut(s) 6, 825, 990, 1933
Eco53kI GAGCTC 1 cut(s) 1282
Eco57I CTGAAG 2 cut(s) 408, 753
Eco81I CCTNAGG 1 cut(s) 2121
EcoICRI GAGCTC 1 cut(s) 1282
EcoRI GAATTC 1 cut(s) 697
EcoRII CCWGG 4 cut(s) 222, 830, 1590, 1838
EcoRV GATATC 4 cut(s) 6, 825, 990, 1933
EcoT14I CCWWGG 1 cut(s) 2184
EcoT22I ATGCAT 4 cut(s) 155, 1411, 1871, 1986
EcoT38I GRGCYC 1 cut(s) 1284
ErhI CCWWGG 1 cut(s) 2184
Esp3I CGTCTC 1 cut(s) 2072
FaeI CATG 9 cut(s) 157, 784, 924, 979, 1062, 1925, 1984, 1988, 2262
FalI AAGNNNNNCTT 4 cut(s) 578, 610, 669, 701
FaqI GGGAC 1 cut(s) 2041
FatI CATG 9 cut(s) 153, 780, 920, 975, 1058, 1921, 1980, 1984, 2258
FauI CCCGC 1 cut(s) 812
FauNDI CATATG 2 cut(s) 874, 1411
FbaI TGATCA 1 cut(s) 1414
FblI GTMKAC 2 cut(s) 1117, 1669
Fnu4HI GCNGC 4 cut(s) 114, 202, 363, 2127
FriOI GRGCYC 1 cut(s) 1284
Fsp4HI GCNGC 4 cut(s) 114, 202, 363, 2127
FspBI CTAG 7 cut(s) 210, 1052, 1086, 1649, 1892, 2130, 2145
GlaI GCGC 1 cut(s) 1958
GluI GCNGC 4 cut(s) 114, 202, 363, 2127
GsaI CCCAGC 1 cut(s) 402
GsuI CTGGAG 1 cut(s) 1032
HaeIII GGCC 2 cut(s) 947, 1838
HhaI GCGC 1 cut(s) 1959
Hin1II CATG 9 cut(s) 157, 784, 924, 979, 1062, 1925, 1984, 1988, 2262
Hin6I GCGC 1 cut(s) 1957
HinP1I GCGC 1 cut(s) 1957
HindIII AAGCTT 2 cut(s) 1347, 2178
HphI GGTGA 4 cut(s) 578, 1009, 1514, 2235
Hpy166II GTNNAC 6 cut(s) 43, 231, 1002, 1118, 1588, 1670
Hpy8I GTNNAC 6 cut(s) 43, 231, 1002, 1118, 1588, 1670
HpyAV CCTTC 5 cut(s) 397, 973, 1717, 1826, 1946
HpyCH4III ACNGT 5 cut(s) 47, 572, 1559, 1781, 1799
HpyCH4IV ACGT 4 cut(s) 969, 1114, 1509, 2065
HpyF10VI GCNNNNNNNGC 5 cut(s) 210, 1355, 1918, 1956, 2186
HpySE526I ACGT 4 cut(s) 969, 1114, 1509, 2065
Hsp92II CATG 9 cut(s) 157, 784, 924, 979, 1062, 1925, 1984, 1988, 2262
HspAI GCGC 1 cut(s) 1957
Ksp22I TGATCA 1 cut(s) 1414
LguI GCTCTTC 1 cut(s) 480
LmnI GCTCC 4 cut(s) 280, 426, 501, 791
Lsp1109I GCAGC 4 cut(s) 125, 213, 349, 2138
LweI GCATC 9 cut(s) 388, 421, 449, 494, 1396, 1758, 1856, 1921, 2198
MaeI CTAG 7 cut(s) 210, 1052, 1086, 1649, 1892, 2130, 2145
MaeII ACGT 4 cut(s) 969, 1114, 1509, 2065
MaeIII GTNAC 6 cut(s) 566, 572, 776, 1015, 1604, 1970
MfeI CAATTG 1 cut(s) 168
MflI RGATCY 5 cut(s) 333, 536, 591, 1126, 1148
MhlI GDGCHC 4 cut(s) 664, 1284, 1627, 1786
MlyI GAGTC 3 cut(s) 215, 1023, 1646
MmeI TCCRAC 1 cut(s) 258
Mph1103I ATGCAT 4 cut(s) 155, 1411, 1871, 1986
MroXI GAANNNNTTC 1 cut(s) 39
MseI TTAA 7 cut(s) 357, 524, 543, 635, 903, 1320, 1449
MslI CAYNNNNRTG 2 cut(s) 429, 1989
MspCI CTTAAG 1 cut(s) 356
MspR9I CCNGG 4 cut(s) 224, 832, 1592, 1840
MunI CAATTG 1 cut(s) 168
Mva1269I GAATGC 1 cut(s) 57
MvaI CCWGG 4 cut(s) 224, 832, 1592, 1840
MwoI GCNNNNNNNGC 5 cut(s) 210, 1355, 1918, 1956, 2186
NdeI CATATG 2 cut(s) 874, 1411
NlaIII CATG 9 cut(s) 157, 784, 924, 979, 1062, 1925, 1984, 1988, 2262
NlaIV GGNNCC 5 cut(s) 312, 497, 1150, 1764, 1837
NmuCI GTSAC 3 cut(s) 566, 572, 1015
NsiI ATGCAT 4 cut(s) 155, 1411, 1871, 1986
NspI RCATGY 4 cut(s) 157, 784, 924, 1925
PaeI GCATGC 1 cut(s) 1925
PciI ACATGT 1 cut(s) 780
PciSI GCTCTTC 1 cut(s) 480
PctI GAATGC 1 cut(s) 57
PdmI GAANNNNTTC 1 cut(s) 39
PfeI GAWTC 9 cut(s) 16, 160, 298, 458, 961, 1214, 1292, 1538, 2102
PflMI CCANNNNNTGG 1 cut(s) 971
PkrI GCNGC 4 cut(s) 115, 203, 364, 2128
PleI GAGTC 3 cut(s) 214, 1022, 1646
PpsI GAGTC 3 cut(s) 214, 1022, 1646
PscI ACATGT 1 cut(s) 780
Psp124BI GAGCTC 1 cut(s) 1284
Psp1406I AACGTT 1 cut(s) 969
Psp6I CCWGG 4 cut(s) 222, 830, 1590, 1838
PspFI CCCAGC 1 cut(s) 398
PspGI CCWGG 4 cut(s) 222, 830, 1590, 1838
PspN4I GGNNCC 5 cut(s) 312, 497, 1150, 1764, 1837
PspPI GGNCC 2 cut(s) 946, 1836
PstI CTGCAG 1 cut(s) 1601
PsuI RGATCY 5 cut(s) 333, 536, 591, 1126, 1148
RsaI GTAC 4 cut(s) 232, 798, 842, 1003
RsaNI GTAC 4 cut(s) 231, 797, 841, 1002
RseI CAYNNNNRTG 2 cut(s) 429, 1989
SacI GAGCTC 1 cut(s) 1284
SapI GCTCTTC 1 cut(s) 480
SaqAI TTAA 7 cut(s) 357, 524, 543, 635, 903, 1320, 1449
SatI GCNGC 4 cut(s) 114, 202, 363, 2127
Sau96I GGNCC 2 cut(s) 946, 1836
SchI GAGTC 3 cut(s) 215, 1023, 1646
ScrFI CCNGG 4 cut(s) 224, 832, 1592, 1840
SduI GDGCHC 4 cut(s) 664, 1284, 1627, 1786
SfaNI GCATC 9 cut(s) 388, 421, 449, 494, 1396, 1758, 1856, 1921, 2198
SfcI CTRYAG 1 cut(s) 1597
SmiI ATTTAAAT 1 cut(s) 1450
SmiMI CAYNNNNRTG 2 cut(s) 429, 1989
SmlI CTYRAG 6 cut(s) 250, 356, 467, 804, 1344, 2071
SmoI CTYRAG 6 cut(s) 250, 356, 467, 804, 1344, 2071
SphI GCATGC 1 cut(s) 1925
SsiI CCGC 1 cut(s) 819
SspI AATATT 3 cut(s) 639, 1273, 2220
SspMI CTAG 7 cut(s) 210, 1052, 1086, 1649, 1892, 2130, 2145
SstI GAGCTC 1 cut(s) 1284
StyD4I CCNGG 4 cut(s) 222, 830, 1590, 1838
StyI CCWWGG 1 cut(s) 2184
SwaI ATTTAAAT 1 cut(s) 1450
TaaI ACNGT 5 cut(s) 47, 572, 1559, 1781, 1799
TaiI ACGT 4 cut(s) 972, 1117, 1512, 2068
TaqI TCGA 3 cut(s) 14, 580, 1710
TatI WGTACW 1 cut(s) 230
TfiI GAWTC 9 cut(s) 16, 160, 298, 458, 961, 1214, 1292, 1538, 2102
Tru1I TTAA 7 cut(s) 357, 524, 543, 635, 903, 1320, 1449
Tru9I TTAA 7 cut(s) 357, 524, 543, 635, 903, 1320, 1449
TscAI CASTG 8 cut(s) 50, 321, 577, 731, 754, 1786, 1903, 2168
TseFI GTSAC 3 cut(s) 566, 572, 1015
TseI GCWGC 4 cut(s) 113, 201, 362, 2126
Tsp45I GTSAC 3 cut(s) 566, 572, 1015
TspDTI ATGAA 6 cut(s) 48, 891, 945, 992, 1118, 1845
TspRI CASTG 8 cut(s) 50, 321, 577, 731, 754, 1786, 1903, 2168
Van91I CCANNNNNTGG 1 cut(s) 971
Vha464I CTTAAG 1 cut(s) 356
XapI RAATTY 8 cut(s) 84, 445, 697, 809, 1259, 1394, 1575, 1689
XbaI TCTAGA 1 cut(s) 1648
XceI RCATGY 4 cut(s) 157, 784, 924, 1925
XmiI GTMKAC 2 cut(s) 1117, 1669
XmnI GAANNNNTTC 1 cut(s) 39
XspI CTAG 7 cut(s) 210, 1052, 1086, 1649, 1892, 2130, 2145
Zsp2I ATGCAT 4 cut(s) 155, 1411, 1871, 1986
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.