Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0002237

Physical Location & Seq

Genomic Coordinates Reverse (-)

15574379 .. 15597893

Gene Structure 23515 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000017354

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3072 bp

ATGGCACTATCATTTGCACTGGATTTAATCGGAGGACAAGTATTTGAGCTGTTGTCTGAAAGTGTTACGCGAGCAGTGGACAGGAAAGCTGCGTTTGGAGACCTTCTCCAAGATATGAAATACACACTGAAGTCCTTAAGAAGACAGGCCGTGGTGATTCAACAGATACAAGAGTATAATCTGGAACTGAGTCTCCCAAATGATGAAATGATTGCTCTTGTAACACAAATGACAGTTGGAAGACAACTCATTGATAGGTTGTCAGGCATTAGATTTCGTTTGTGCAACTTCTGCTGCATAAATGATTTGGCCGACCAACTTAAAGAATTGAATAGTTCTCTTGAAAGTCTGTTTGACAACCTGCAGTTGGAGCAAGTAAGGGATGCTAAGGAGACCTTGAAATTGTCAAGGCAAAACCGCGATGATCTTGAGAAAATGAAGGTCATGTTGGAGAAGATACTGCAGATGATTGAAGATCCTGAAGTGAAGGGTCTACTGAAGAAGTTAGTGGAGCAGAATGCAGATGGATCAATGAAGAGCTCGACCTCAACCTCAACCTTGGGCTCAGGAAGTTGCACAGAAAATGAAGGGAATTGCGTAACAAAAGTGCCATCATCAAAATCACTGGTAGAAGCAGCATTTCGGGTACTACTTAATGCCGTTATAGAAGTGAAGGAGAGCACTATGATGTTTAGACCTGTCCTCCAACGTCTCAAATCTACTCTAGACTATTTACTGCCATTTATTGAAGATATGGTACAATGTAACAAGGCATTAGATCTTCCAAAAGAGGAACTAGAAAATATGAGAATACAGATGGAGAATGGTGTAATGCTTGTTGGCAAGTGCTTACATGTTCGTCGGTGGACAAGGAACAAAAAGTATATATACACCAACCAACTTTTTGAACTGGATGAATATCTTCAAGGGCAGTTAAGTATACTGCTAGTTCAGATAGCAAGGGATGTGAGGGAGACCTCGGTGTTGATAAGGAATATTAAGGAGGTGGTAGGGAGAAATAAAAGGAGTGGTATGGCAGAAAATCAACTTGAATATAAAGGTTGTTGTAATGTATCTCAACCTCCACTCCCTACTGTTGTATTCGTAACAAACATGCAAGGAACAAGGGATGTGAGGATGGAGACGTTGGTTTCTGTAAAGAATATCGAGGAGGGGGTCAAAGGAATGGAAGGGAAAAGTACGGTAGATGAAGTATCTGAAACTTTATCGCCTACTGTTGGAATTGAAGTACTCAACATGCAGGGAACAAAGGATGTGATCAAGGAGACACAGGCTTCAACAGAGAATACAAATGCAGAGGTCAATCTAATTGAAGGGAGAGCTGAGGCCCAGCTTGGCTCCATTGCATCTTCAGAGAGACAACAGCTGACAGAGGGAATCAGTGAAGTACATGGAACTTTAGGTCACTGTGAACTGGAAAATTCAGTATCTCAAGAGACCTTTTCTCCATGCCTTCCTAGTTCCATCCCTCTAGAGTTCAATGCATTGAAGAAGGAAAATGTATTTCCTGAGAGACCTGGCCAGCCTGACTGCCTTGATAAGGAGTACAAAATCAACTCTACAGTGAAAGAGCCTGTTTCCTTTAAGGTTGATCATTCAATGTACATTGAACTTTGGAAGTGGGTGGATAGAATTTCAAAGATATTTCCCGACATAGAAGCAGCAAGAACTCGAAGCGCAACGGGAATACAGGCTCTATGCATGTTGAATTGTGCAATTGAGAAAGCCAAGCAACTTGTTCAGTATTGCAGCGAGTCTAGCAAACTCGACCCGTTGATGATCCTTAAGGCTGCAACATTTAAACTGGATCCATCTAAAGAAGAGGCCGGCAAGGTCATCCGAGAATTGCTCCTTCTGCATCAAATTCAAAAGAAGATTCTGAACTCAAGATCTATTAAGACGTTGCTACAAAAAGTTGCTGACTGCACATGGAAACTTAATATTAGTCTATTAGATGAGCAAACAGGGAGTGCCCCTGTGAGGCAACAAGGATCACTTGCTTCTTTGAATTCTGGCGGTGTTGAAACTCCTGAGGAACAAAGTAACACGTTGCTTAGATCCGTACCTCGTGAGGAGTTTTCAAGCGGAACTTCCAGATTGATGTACGAACCTGGACGTGGATACAGAAGACAGAAGTGGTTTGATGAGGGTCATGATACTGGCCCAAAGGCTAATACTGAACTAGCCGACCTTTCATTGACTCCAAATAATGCCATGAAGGGGTCAATATCAGAGTGGTTTTCGATGTATGGAATCACCATTCCAACCCCAAGTATGGAACTAGAAACCCTTGACTCACGGGAAAACAGTTCCATTGATAGTTCAGTCAGTTCTACCAATAAGACAGATGGCTTGGGTTTGGTTCAGACTGATGATGATTCTCTAAAATACCAATATGCTAAAAAATGTGAGACAGATTCGAAATGTCTGTCTAATATTAGTAAACTCAAATGGGAAGACCAATGCAAGGTCGTTGACGACGTTCAAGACCATTTGAAATGCAATGCTGAAGCTTTGGATTCTATGTCATCTGAGAATTTTGCTGAACCTCTCATCAGTTTTTTAAGGGATGCACAGGATCTGGACGATGTAAAAGCTCAGAGAGATGGATTTCAATTATTACCGGCCTTTGTGAGCAAAAACAGGATTTCTATTGCTGAAACTGATGGATGCATTGGTTCTGTTGTTGAAGTCCTAAATACTGGAAGTAGAGAGCATCAAGAGCACGCGGTTGATATCCTCCTTTCTTTATGCTCTGAGAGCATTGACTATCGTCGCTTGGTCATGCATGAGGGTGTTATTCCTGCTCTTGTCCTTCCATCTACAAATGGGACAGAGAGAGCAAGGGTAACTTCAATGGAAGTTCTACGTCTCTTAAGAGATACTGATTATGTCCATGAGCAAGAATCTGCTGAACCTGAGCCTGAGGCAGCTAGAGACGATGTAAATCACTCAAATGCAGAGAAAGCATCAAAGGCATCAGGAATTTTCGGAAAGAAAATATTGATTTTCTCAATACCTAGTTCCCTTTCACCAGGAAATATGGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

KAP U-box

Protein Analysis

1024

Amino Acids

114.3

Weight (kDa)

5.14

Isoelectric Point (pI)

42.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RPW8	PF05659	12 - 143	1.1e-11	Arabidopsis broad-spectrum mildew resistance protein RPW8
RPW8	PF05659	210 - 329	3.5e-23	Arabidopsis broad-spectrum mildew resistance protein RPW8

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002237)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G36550
fragaria_vesca	FvH4_1g13090 FvH4_1g13090
malus_domestica	MD02G1145600.v1.1 MD15G1259700.v1.1
prunus_persica	Prupe.7G156200_v2.0.a1 Prupe.7G156200_v2.0.a1 Prupe.7G156200_v2.0.a1 Prupe.7G156200_v2.0.a1 Prupe.7G156200_v2.0.a1 Prupe.7G156200_v2.0.a1 Prupe.7G156200_v2.0.a1 Prupe.7G156200_v2.0.a1 Prupe.7G156200_v2.0.a1 Prupe.7G156200_v2.0.a1 Prupe.7G156200_v2.0.a1
pyrus_communis	pycom02g11520 pycom15g22690
rosa_chinensis	RchiOBHm_Chr2g0101271 RchiOBHm_Chr2g0104721 RchiOBHm_Chr2g0104731
rosa_laevigata	RLG00000017066 RLG00000017354
rosa_multiflora	Rmu_sc0006314.1_g000019
rosa_roxburghii	Rroxscaffold_2G00141450
rosa_rugosa	Rorug02G0099600 Rorug02G0122000 Rorug02G0122100 Rorug02G0122200
rosa_samantha	Rh2AG145600 Rh2AG172300 Rh2BG151000 Rh2BG179900 Rh2CG151400 Rh2CG177400 Rh2DG151100 Rh2DG178600
rosa_wichuraiana	Rw0G008640 Rw0G008650 Rw2G011360 Rw2G013490 Rw2G013550 Rw2G013560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	369
AccI	GTMKAC	2 cut(s)	493, 940
AccII	CGCG	3 cut(s)	70, 420, 2750
AciI	CCGC	4 cut(s)	418, 2037, 2106, 2750
AclWI	GGATC	8 cut(s)	470, 535, 1795, 1823, 1836, 2020, 2073, 2607
AcoI	YGGCCR	2 cut(s)	309, 1540
AcsI	RAATTY	6 cut(s)	1441, 1653, 1884, 2029, 2557, 3006
AcuI	CTGAAG	5 cut(s)	149, 501, 518, 1356, 2550
AfaI	GTAC	9 cut(s)	648, 759, 1201, 1251, 1410, 1568, 1625, 2085, 2126
AfiI	CCNNNNNNNGG	8 cut(s)	367, 1238, 1561, 2001, 2138, 2241, 2296, 2488
AflII	CTTAAG	3 cut(s)	136, 1805, 2896
AflIII	ACRYGT	2 cut(s)	853, 2067
AjiI	CACGTC	1 cut(s)	2138
AjnI	CCWGG	3 cut(s)	1537, 2131, 3055
AjuI	GAANNNNNNNTTGG	2 cut(s)	431, 463
AloI	GAACNNNNNNTCC	2 cut(s)	177, 209
AluBI	AGCT	9 cut(s)	49, 89, 540, 1343, 1354, 1387, 2534, 2618, 2954
AluI	AGCT	9 cut(s)	49, 89, 540, 1343, 1354, 1387, 2534, 2618, 2954
Alw21I	GWGCWC	3 cut(s)	542, 683, 2748
AlwI	GGATC	8 cut(s)	470, 535, 1795, 1823, 1836, 2020, 2073, 2607
AlwNI	CAGNNNCTG	1 cut(s)	2602
AoxI	GGCC	7 cut(s)	147, 309, 1347, 1540, 1845, 2182, 2646
ApeKI	GCWGC	7 cut(s)	89, 294, 635, 1682, 1770, 1811, 2951
ApoI	RAATTY	6 cut(s)	1441, 1653, 1884, 2029, 2557, 3006
ArsI	GACNNNNNNTTYG	2 cut(s)	2435, 2467
Asp700I	GAANNNNTTC	1 cut(s)	921
AspLEI	GCGC	1 cut(s)	1700
AspS9I	GGNCC	2 cut(s)	1348, 2183
AsuHPI	GGTGA	3 cut(s)	166, 2269, 3045
AsuII	TTCGAA	1 cut(s)	2441
AxyI	CCTNAGG	2 cut(s)	2052, 2946
BaeGI	GKGCMC	1 cut(s)	1996
BaeI	ACNNNNGTAYC	2 cut(s)	158, 191
BalI	TGGCCA	1 cut(s)	1542
BamHI	GGATCC	1 cut(s)	1828
BanII	GRGCYC	2 cut(s)	542, 566
BarI	GAAGNNNNNNTAC	2 cut(s)	741, 773
BauI	CACGAG	1 cut(s)	2088
BbsI	GAAGAC	4 cut(s)	148, 247, 2155, 2484
Bbv12I	GWGCWC	3 cut(s)	542, 683, 2748
BbvCI	CCTCAGC	1 cut(s)	1344
BbvI	GCAGC	7 cut(s)	76, 281, 647, 1694, 1782, 1798, 2963
BceAI	ACGGC	2 cut(s)	134, 644
BciT130I	CCWGG	3 cut(s)	1539, 2133, 3057
BciVI	GTATCC	1 cut(s)	2135
BclI	TGATCA	2 cut(s)	1278, 1612
BfmI	CTRYAG	3 cut(s)	362, 461, 1581
BfrI	CTTAAG	3 cut(s)	136, 1805, 2896
BfuAI	ACCTGC	1 cut(s)	369
BfuI	GTATCC	1 cut(s)	2135
BglII	AGATCT	2 cut(s)	778, 1910
BisI	GCNGC	7 cut(s)	90, 295, 636, 1683, 1771, 1812, 2952
BlsI	GCNGC	7 cut(s)	91, 296, 637, 1684, 1772, 1813, 2953
BmcAI	AGTACT	1 cut(s)	1251
Bme1390I	CCNGG	3 cut(s)	1539, 2133, 3057
BmgBI	CACGTC	1 cut(s)	2138
BmgT120I	GGNCC	2 cut(s)	1348, 2183
BmiI	GGNNCC	2 cut(s)	1360, 1830
BmrFI	CCNGG	3 cut(s)	1539, 2133, 3057
BmsI	GCATC	8 cut(s)	373, 1376, 1888, 2581, 2681, 2746, 2999, 3008
BoxI	GACNNNNGTC	1 cut(s)	2793
BpiI	GAAGAC	4 cut(s)	148, 247, 2155, 2484
BplI	GAGNNNNNCTC	4 cut(s)	90, 122, 1854, 1886
Bpu10I	CCTNAGC	4 cut(s)	387, 565, 1344, 2940
Bpu14I	TTCGAA	1 cut(s)	2441
BpuEI	CTTGAG	3 cut(s)	449, 1437, 1891
BsaBI	GATNNNNATC	2 cut(s)	918, 2967
BsaI	GGTCTC	5 cut(s)	93, 386, 968, 1451, 1528
BsaJI	CCNNGG	3 cut(s)	150, 558, 978
Bsc4I	CCNNNNNNNGG	8 cut(s)	367, 1238, 1561, 2001, 2138, 2241, 2296, 2488
Bse118I	RCCGGY	2 cut(s)	1847, 2644
Bse1I	ACTGG	7 cut(s)	24, 630, 915, 1440, 1830, 2185, 2728
Bse21I	CCTNAGG	2 cut(s)	2052, 2946
Bse3DI	GCAATG	2 cut(s)	1362, 2530
Bse8I	GATNNNNATC	2 cut(s)	918, 2967
BseBI	CCWGG	3 cut(s)	1539, 2133, 3057
BseDI	CCNNGG	3 cut(s)	150, 558, 978
BseJI	GATNNNNATC	2 cut(s)	918, 2967
BseLI	CCNNNNNNNGG	8 cut(s)	367, 1238, 1561, 2001, 2138, 2241, 2296, 2488
BseMI	GCAATG	2 cut(s)	1362, 2530
BseNI	ACTGG	7 cut(s)	24, 630, 915, 1440, 1830, 2185, 2728
BseRI	GAGGAG	2 cut(s)	1184, 2108
BseSI	GKGCMC	1 cut(s)	1996
BseXI	GCAGC	7 cut(s)	76, 281, 647, 1694, 1782, 1798, 2963
BseYI	CCCAGC	1 cut(s)	1350
BsgI	GTGCAG	1 cut(s)	1930
Bsh1236I	CGCG	3 cut(s)	70, 420, 2750
BshFI	GGCC	7 cut(s)	149, 311, 1349, 1542, 1847, 2184, 2648
BsiHKAI	GWGCWC	3 cut(s)	542, 683, 2748
BsiSI	CCGG	2 cut(s)	1848, 2645
BslFI	GGGAC	1 cut(s)	2866
BslI	CCNNNNNNNGG	8 cut(s)	367, 1238, 1561, 2001, 2138, 2241, 2296, 2488
BsmBI	CGTCTC	4 cut(s)	716, 1136, 2897, 2952
BsmFI	GGGAC	1 cut(s)	2866
BsmI	GAATGC	1 cut(s)	523
BsnI	GGCC	7 cut(s)	149, 311, 1349, 1542, 1847, 2184, 2648
Bso31I	GGTCTC	5 cut(s)	93, 386, 968, 1451, 1528
Bsp119I	TTCGAA	1 cut(s)	2441
Bsp1286I	GDGCHC	5 cut(s)	542, 566, 683, 1996, 2748
Bsp1407I	TGTACA	1 cut(s)	1623
BspACI	CCGC	4 cut(s)	418, 2037, 2106, 2750
BspANI	GGCC	7 cut(s)	149, 311, 1349, 1542, 1847, 2184, 2648
BspFNI	CGCG	3 cut(s)	70, 420, 2750
BspHI	TCATGA	1 cut(s)	2173
BspLI	GGNNCC	2 cut(s)	1360, 1830
BspMAI	CTGCAG	2 cut(s)	366, 465
BspMI	ACCTGC	1 cut(s)	369
BspPI	GGATC	8 cut(s)	470, 535, 1795, 1823, 1836, 2020, 2073, 2607
BspQI	GCTCTTC	1 cut(s)	530
BspT104I	TTCGAA	1 cut(s)	2441
BspTI	CTTAAG	3 cut(s)	136, 1805, 2896
BspTNI	GGTCTC	5 cut(s)	93, 386, 968, 1451, 1528
BsrDI	GCAATG	2 cut(s)	1362, 2530
BsrFI	RCCGGY	2 cut(s)	1847, 2644
BsrGI	TGTACA	1 cut(s)	1623
BsrI	ACTGG	7 cut(s)	24, 630, 915, 1440, 1830, 2185, 2728
BssAI	RCCGGY	2 cut(s)	1847, 2644
BssECI	CCNNGG	3 cut(s)	150, 558, 978
BssNAI	GTATAC	1 cut(s)	941
BssSI	CACGAG	1 cut(s)	2088
BssT1I	CCWWGG	1 cut(s)	558
Bst1107I	GTATAC	1 cut(s)	941
Bst2BI	CACGAG	1 cut(s)	2088
Bst2UI	CCWGG	3 cut(s)	1539, 2133, 3057
Bst4CI	ACNGT	7 cut(s)	235, 1096, 1204, 1237, 1430, 1585, 2330
Bst6I	CTCTTC	2 cut(s)	530, 1836
BstAFI	CTTAAG	3 cut(s)	136, 1805, 2896
BstAPI	GCANNNNNTGC	1 cut(s)	291
BstAUI	TGTACA	1 cut(s)	1623
BstBI	TTCGAA	1 cut(s)	2441
BstC8I	GCNNGC	4 cut(s)	72, 1544, 1849, 2748
BstDSI	CCRYGG	1 cut(s)	150
BstENI	CCTNNNNNAGG	1 cut(s)	1559
BstFNI	CGCG	3 cut(s)	70, 420, 2750
BstHHI	GCGC	1 cut(s)	1700
BstMWI	GCNNNNNNNGC	8 cut(s)	291, 370, 1779, 1876, 2743, 2781, 2987, 2996
BstNI	CCWGG	3 cut(s)	1539, 2133, 3057
BstNSI	RCATGY	4 cut(s)	857, 1117, 1261, 1726
BstPAI	GACNNNNGTC	1 cut(s)	2793
BstSCI	CCNGG	3 cut(s)	1537, 2131, 3055
BstSFI	CTRYAG	3 cut(s)	362, 461, 1581
BstSLI	GKGCMC	1 cut(s)	1996
BstUI	CGCG	3 cut(s)	70, 420, 2750
BstV1I	GCAGC	7 cut(s)	76, 281, 647, 1694, 1782, 1798, 2963
BstV2I	GAAGAC	4 cut(s)	148, 247, 2155, 2484
BstX2I	RGATCY	6 cut(s)	475, 778, 1828, 1910, 2078, 2599
BstYI	RGATCY	6 cut(s)	475, 778, 1828, 1910, 2078, 2599
BstZ17I	GTATAC	1 cut(s)	941
Bsu36I	CCTNAGG	2 cut(s)	2052, 2946
BsuI	GTATCC	1 cut(s)	2135
BsuRI	GGCC	7 cut(s)	149, 311, 1349, 1542, 1847, 2184, 2648
BtgI	CCRYGG	1 cut(s)	150
BtgZI	GCGATG	1 cut(s)	435
BtrI	CACGTC	1 cut(s)	2138
BtsI	GCAGTG	1 cut(s)	81
BtsIMutI	CAGTG	7 cut(s)	17, 81, 125, 623, 1408, 1426, 1590
BveI	ACCTGC	1 cut(s)	369
Cac8I	GCNNGC	4 cut(s)	72, 1544, 1849, 2748
CaiI	CAGNNNCTG	1 cut(s)	2602
CciI	TCATGA	1 cut(s)	2173
CfoI	GCGC	1 cut(s)	1700
Cfr10I	RCCGGY	2 cut(s)	1847, 2644
Cfr13I	GGNCC	2 cut(s)	1348, 2183
Csp6I	GTAC	9 cut(s)	647, 758, 1200, 1250, 1409, 1567, 1624, 2084, 2125
CviQI	GTAC	9 cut(s)	647, 758, 1200, 1250, 1409, 1567, 1624, 2084, 2125
DraI	TTTAAA	1 cut(s)	1822
EaeI	YGGCCR	2 cut(s)	309, 1540
Eam1104I	CTCTTC	2 cut(s)	530, 1836
EarI	CTCTTC	2 cut(s)	530, 1836
Ecl136II	GAGCTC	1 cut(s)	540
Eco130I	CCWWGG	1 cut(s)	558
Eco24I	GRGCYC	2 cut(s)	542, 566
Eco31I	GGTCTC	5 cut(s)	93, 386, 968, 1451, 1528
Eco32I	GATATC	1 cut(s)	2758
Eco53kI	GAGCTC	1 cut(s)	540
Eco57I	CTGAAG	5 cut(s)	149, 501, 518, 1356, 2550
Eco81I	CCTNAGG	2 cut(s)	2052, 2946
EcoICRI	GAGCTC	1 cut(s)	540
EcoNI	CCTNNNNNAGG	1 cut(s)	1559
EcoRI	GAATTC	1 cut(s)	2029
EcoRII	CCWGG	3 cut(s)	1537, 2131, 3055
EcoRV	GATATC	1 cut(s)	2758
EcoT14I	CCWWGG	1 cut(s)	558
EcoT22I	ATGCAT	4 cut(s)	1507, 1724, 2696, 2811
EcoT38I	GRGCYC	2 cut(s)	542, 566
ErhI	CCWWGG	1 cut(s)	558
Esp3I	CGTCTC	4 cut(s)	716, 1136, 2897, 2952
FalI	AAGNNNNNCTT	8 cut(s)	380, 412, 2001, 2033, 2095, 2127, 2857, 2889
FaqI	GGGAC	1 cut(s)	2866
FbaI	TGATCA	2 cut(s)	1278, 1612
FblI	GTMKAC	2 cut(s)	493, 940
Fnu4HI	GCNGC	7 cut(s)	90, 295, 636, 1683, 1771, 1812, 2952
FriOI	GRGCYC	2 cut(s)	542, 566
Fsp4HI	GCNGC	7 cut(s)	90, 295, 636, 1683, 1771, 1812, 2952
GlaI	GCGC	1 cut(s)	1699
GluI	GCNGC	7 cut(s)	90, 295, 636, 1683, 1771, 1812, 2952
GsaI	CCCAGC	1 cut(s)	1354
HaeIII	GGCC	7 cut(s)	149, 311, 1349, 1542, 1847, 2184, 2648
HapII	CCGG	2 cut(s)	1848, 2645
HhaI	GCGC	1 cut(s)	1700
Hin6I	GCGC	1 cut(s)	1698
HinP1I	GCGC	1 cut(s)	1698
HincII	GTYRAC	1 cut(s)	2497
HindII	GTYRAC	1 cut(s)	2497
HindIII	AAGCTT	1 cut(s)	2532
HpaII	CCGG	2 cut(s)	1848, 2645
HphI	GGTGA	3 cut(s)	166, 2269, 3045
Hpy166II	GTNNAC	7 cut(s)	79, 494, 867, 941, 1433, 2465, 2497
Hpy8I	GTNNAC	7 cut(s)	79, 494, 867, 941, 1433, 2465, 2497
Hpy99I	CGWCG	3 cut(s)	864, 2504, 2799
HpyCH4III	ACNGT	7 cut(s)	235, 1096, 1204, 1237, 1430, 1585, 2330
HpyCH4IV	ACGT	7 cut(s)	709, 1145, 1922, 2069, 2137, 2502, 2890
HpyF10VI	GCNNNNNNNGC	8 cut(s)	291, 370, 1779, 1876, 2743, 2781, 2987, 2996
HpySE526I	ACGT	7 cut(s)	709, 1145, 1922, 2069, 2137, 2502, 2890
HspAI	GCGC	1 cut(s)	1698
KroI	GCCGGC	1 cut(s)	1847
KroNI	GCCGGC	1 cut(s)	1849
Ksp22I	TGATCA	2 cut(s)	1278, 1612
LguI	GCTCTTC	1 cut(s)	530
LmnI	GCTCC	4 cut(s)	370, 511, 1364, 1875
Lsp1109I	GCAGC	7 cut(s)	76, 281, 647, 1694, 1782, 1798, 2963
LweI	GCATC	8 cut(s)	373, 1376, 1888, 2581, 2681, 2746, 2999, 3008
MaeII	ACGT	7 cut(s)	709, 1145, 1922, 2069, 2137, 2502, 2890
MaeIII	GTNAC	8 cut(s)	64, 220, 598, 764, 1105, 1424, 2063, 2869
MfeI	CAATTG	1 cut(s)	1737
MflI	RGATCY	6 cut(s)	475, 778, 1828, 1910, 2078, 2599
MhlI	GDGCHC	5 cut(s)	542, 566, 683, 1996, 2748
MlsI	TGGCCA	1 cut(s)	1542
MluNI	TGGCCA	1 cut(s)	1542
MlyI	GAGTC	4 cut(s)	199, 1784, 2215, 2309
MmeI	TCCRAC	6 cut(s)	217, 348, 429, 730, 1219, 2309
Mox20I	TGGCCA	1 cut(s)	1542
Mph1103I	ATGCAT	4 cut(s)	1507, 1724, 2696, 2811
MroNI	GCCGGC	1 cut(s)	1847
MroXI	GAANNNNTTC	1 cut(s)	921
MscI	TGGCCA	1 cut(s)	1542
MslI	CAYNNNNRTG	3 cut(s)	686, 2814, 2976
Msp20I	TGGCCA	1 cut(s)	1542
MspA1I	CMGCKG	1 cut(s)	1387
MspCI	CTTAAG	3 cut(s)	136, 1805, 2896
MspI	CCGG	2 cut(s)	1848, 2645
MspR9I	CCNGG	3 cut(s)	1539, 2133, 3057
MunI	CAATTG	1 cut(s)	1737
Mva1269I	GAATGC	1 cut(s)	523
MvaI	CCWGG	3 cut(s)	1539, 2133, 3057
MvnI	CGCG	3 cut(s)	70, 420, 2750
MwoI	GCNNNNNNNGC	8 cut(s)	291, 370, 1779, 1876, 2743, 2781, 2987, 2996
NaeI	GCCGGC	1 cut(s)	1849
NgoMIV	GCCGGC	1 cut(s)	1847
NlaIV	GGNNCC	2 cut(s)	1360, 1830
NmuCI	GTSAC	1 cut(s)	1424
NsiI	ATGCAT	4 cut(s)	1507, 1724, 2696, 2811
NspI	RCATGY	4 cut(s)	857, 1117, 1261, 1726
NspV	TTCGAA	1 cut(s)	2441
PagI	TCATGA	1 cut(s)	2173
PciI	ACATGT	1 cut(s)	853
PciSI	GCTCTTC	1 cut(s)	530
PcsI	WCGNNNNNNNCGW	1 cut(s)	2499
PctI	GAATGC	1 cut(s)	523
PdiI	GCCGGC	1 cut(s)	1849
PdmI	GAANNNNTTC	1 cut(s)	921
PfeI	GAWTC	8 cut(s)	157, 1398, 1897, 2274, 2399, 2438, 2540, 2927
PkrI	GCNGC	7 cut(s)	91, 296, 637, 1684, 1772, 1813, 2953
PleI	GAGTC	4 cut(s)	198, 1783, 2215, 2309
PpsI	GAGTC	4 cut(s)	198, 1783, 2215, 2309
PscI	ACATGT	1 cut(s)	853
PshAI	GACNNNNGTC	1 cut(s)	2793
Psp124BI	GAGCTC	1 cut(s)	542
Psp6I	CCWGG	3 cut(s)	1537, 2131, 3055
PspFI	CCCAGC	1 cut(s)	1350
PspGI	CCWGG	3 cut(s)	1537, 2131, 3055
PspN4I	GGNNCC	2 cut(s)	1360, 1830
PspPI	GGNCC	2 cut(s)	1348, 2183
PstI	CTGCAG	2 cut(s)	366, 465
PstNI	CAGNNNCTG	1 cut(s)	2602
PsuI	RGATCY	6 cut(s)	475, 778, 1828, 1910, 2078, 2599
PvuII	CAGCTG	1 cut(s)	1387
RsaI	GTAC	9 cut(s)	648, 759, 1201, 1251, 1410, 1568, 1625, 2085, 2126
RsaNI	GTAC	9 cut(s)	647, 758, 1200, 1250, 1409, 1567, 1624, 2084, 2125
RseI	CAYNNNNRTG	3 cut(s)	686, 2814, 2976
SacI	GAGCTC	1 cut(s)	542
SapI	GCTCTTC	1 cut(s)	530
SatI	GCNGC	7 cut(s)	90, 295, 636, 1683, 1771, 1812, 2952
Sau96I	GGNCC	2 cut(s)	1348, 2183
ScaI	AGTACT	1 cut(s)	1251
SchI	GAGTC	4 cut(s)	199, 1784, 2215, 2309
ScrFI	CCNGG	3 cut(s)	1539, 2133, 3057
SduI	GDGCHC	5 cut(s)	542, 566, 683, 1996, 2748
SfaNI	GCATC	8 cut(s)	373, 1376, 1888, 2581, 2681, 2746, 2999, 3008
SfcI	CTRYAG	3 cut(s)	362, 461, 1581
SfuI	TTCGAA	1 cut(s)	2441
SmiMI	CAYNNNNRTG	3 cut(s)	686, 2814, 2976
SmlI	CTYRAG	6 cut(s)	136, 428, 1452, 1805, 1906, 2896
SmoI	CTYRAG	6 cut(s)	136, 428, 1452, 1805, 1906, 2896
SsiI	CCGC	4 cut(s)	418, 2037, 2106, 2750
SspI	AATATT	4 cut(s)	997, 1963, 2458, 3024
SstI	GAGCTC	1 cut(s)	542
StyD4I	CCNGG	3 cut(s)	1537, 2131, 3055
StyI	CCWWGG	1 cut(s)	558
TaaI	ACNGT	7 cut(s)	235, 1096, 1204, 1237, 1430, 1585, 2330
TaiI	ACGT	7 cut(s)	712, 1148, 1925, 2072, 2140, 2505, 2893
TaqI	TCGA	6 cut(s)	542, 1167, 1693, 1788, 2264, 2441
TatI	WGTACW	4 cut(s)	1249, 1408, 1566, 1623
TfiI	GAWTC	8 cut(s)	157, 1398, 1897, 2274, 2399, 2438, 2540, 2927
TscAI	CASTG	7 cut(s)	24, 81, 132, 630, 1408, 1433, 1590
TseFI	GTSAC	1 cut(s)	1424
TseI	GCWGC	7 cut(s)	89, 294, 635, 1682, 1770, 1811, 2951
Tsp45I	GTSAC	1 cut(s)	1424
TspDTI	ATGAA	9 cut(s)	131, 219, 452, 548, 600, 930, 1224, 2205, 2252
TspGWI	ACGGA	1 cut(s)	2071
TspRI	CASTG	7 cut(s)	24, 81, 132, 630, 1408, 1433, 1590
Vha464I	CTTAAG	3 cut(s)	136, 1805, 2896
XagI	CCTNNNNNAGG	1 cut(s)	1559
XapI	RAATTY	6 cut(s)	1441, 1653, 1884, 2029, 2557, 3006
XbaI	TCTAGA	2 cut(s)	724, 1492
XceI	RCATGY	4 cut(s)	857, 1117, 1261, 1726
XmiI	GTMKAC	2 cut(s)	493, 940
XmnI	GAANNNNTTC	1 cut(s)	921
ZrmI	AGTACT	1 cut(s)	1251
Zsp2I	ATGCAT	4 cut(s)	1507, 1724, 2696, 2811

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.