MD15G1312200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002086
Physical Location & Seq
Reverse (-)
31451968 .. 31455126
3159 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1312200.v1.1.491

Sequence Viewer

Length: 531 bp
ATGGCAGTCTCCTTCACTCGTTTGCCATGGTGTTGGTGGGGTGGGACAGAGAAGGAGCCTGCTAACAATGGCTCCTCAGTGAATTCTTCTTTTGAGTGTGGTTTTGGTGGTATGAGGGACCCCGGGACGGTTCAATTCCCTTCCGTTAAGGGCACGAAAATGGCCTCGTCACCGCGAAAGGTTAAGAGGAAATGGCTGAGTAGGGAAGAGAGAAGGGTTGATAGGGAGTATGATATTGTTATTGTACCATCAGATGGTGGTGACATTTTGTCAGGGTCGGAGTCCGATGACTCAGACTGGTCTATTGGGTGGTTGGAGCCTCATGGCCCGGGTTTCCAGAGTGACGATGACAAATCGGATAATAGTTATGCAGTGCTGGTCCCTTGCTACAGCCCTGGCTGCAAGGAGGTGGTGGAGGATTCCAACAATGAGCTCTTGAGTGCCATCAAGAACCTCCCTGATGAATTCTCTTCTGATTGCAAGAACTATATGGAACAGTGGCTTTCTTCTCTTCAGAACTTCGATGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

177

Amino Acids

19.59

Weight (kDa)

4.53

Isoelectric Point (pI)

59.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 254
AccII CGCG 1 cut(s) 175
AciI CCGC 1 cut(s) 173
AcsI RAATTY 2 cut(s) 82, 464
AcuI CTGAAG 1 cut(s) 497
AfaI GTAC 1 cut(s) 246
AfiI CCNNNNNNNGG 2 cut(s) 127, 254
AgsI TTSAA 1 cut(s) 134
AhdI GACNNNNNGTC 1 cut(s) 268
AjnI CCWGG 1 cut(s) 394
AluBI AGCT 1 cut(s) 433
AluI AGCT 1 cut(s) 433
Alw21I GWGCWC 1 cut(s) 435
Alw26I GTCTC 1 cut(s) 13
Ama87I CYCGRG 2 cut(s) 122, 328
AoxI GGCC 2 cut(s) 162, 325
ApeKI GCWGC 1 cut(s) 399
ApoI RAATTY 2 cut(s) 82, 464
AspS9I GGNCC 3 cut(s) 118, 326, 379
AsuC2I CCSGG 4 cut(s) 123, 124, 329, 330
AsuHPI GGTGA 2 cut(s) 162, 272
AvaI CYCGRG 2 cut(s) 122, 328
AvaII GGWCC 2 cut(s) 118, 379
BaeGI GKGCMC 1 cut(s) 155
BanII GRGCYC 1 cut(s) 435
Bbv12I GWGCWC 1 cut(s) 435
BbvI GCAGC 1 cut(s) 386
BccI CCATC 3 cut(s) 248, 256, 452
BciT130I CCWGG 1 cut(s) 396
BcnI CCSGG 4 cut(s) 123, 124, 329, 330
BcoDI GTCTC 1 cut(s) 13
BfmI CTRYAG 1 cut(s) 388
BisI GCNGC 1 cut(s) 400
BlsI GCNGC 1 cut(s) 401
Bme1390I CCNGG 5 cut(s) 123, 124, 329, 330, 396
Bme18I GGWCC 2 cut(s) 118, 379
BmeRI GACNNNNNGTC 1 cut(s) 268
BmeT110I CYCGRG 2 cut(s) 122, 328
BmgT120I GGNCC 3 cut(s) 118, 326, 379
BmiI GGNNCC 6 cut(s) 57, 73, 119, 120, 318, 381
BmrFI CCNGG 5 cut(s) 123, 124, 329, 330, 396
BmsI GCATC 1 cut(s) 514
BpuEI CTTGAG 1 cut(s) 457
BpuMI CCSGG 4 cut(s) 123, 124, 329, 330
BsaJI CCNNGG 5 cut(s) 26, 121, 122, 328, 394
BsaXI ACNNNNNCTCC 2 cut(s) 56, 86
Bsc4I CCNNNNNNNGG 2 cut(s) 127, 254
Bse1I ACTGG 1 cut(s) 302
BseBI CCWGG 1 cut(s) 396
BseDI CCNNGG 5 cut(s) 26, 121, 122, 328, 394
BseLI CCNNNNNNNGG 2 cut(s) 127, 254
BseMII CTCAG 3 cut(s) 90, 188, 306
BseNI ACTGG 1 cut(s) 302
BseRI GAGGAG 1 cut(s) 64
BseSI GKGCMC 1 cut(s) 155
BseXI GCAGC 1 cut(s) 386
Bsh1236I CGCG 1 cut(s) 175
BshFI GGCC 2 cut(s) 164, 327
BsiHKAI GWGCWC 1 cut(s) 435
BsiHKCI CYCGRG 2 cut(s) 122, 328
BsiSI CCGG 2 cut(s) 123, 329
BslFI GGGAC 4 cut(s) 58, 131, 139, 365
BslI CCNNNNNNNGG 2 cut(s) 127, 254
BsmAI GTCTC 1 cut(s) 13
BsmFI GGGAC 4 cut(s) 58, 131, 139, 365
BsnI GGCC 2 cut(s) 164, 327
BsoBI CYCGRG 2 cut(s) 122, 328
Bsp1286I GDGCHC 2 cut(s) 155, 435
Bsp19I CCATGG 1 cut(s) 26
BspACI CCGC 1 cut(s) 173
BspANI GGCC 2 cut(s) 164, 327
BspCNI CTCAG 3 cut(s) 89, 189, 305
BspFNI CGCG 1 cut(s) 175
BspLI GGNNCC 6 cut(s) 57, 73, 119, 120, 318, 381
BsrI ACTGG 1 cut(s) 302
BssECI CCNNGG 5 cut(s) 26, 121, 122, 328, 394
BssT1I CCWWGG 1 cut(s) 26
Bst2UI CCWGG 1 cut(s) 396
Bst4CI ACNGT 2 cut(s) 130, 498
Bst6I CTCTTC 3 cut(s) 201, 475, 516
BstC8I GCNNGC 1 cut(s) 60
BstDEI CTNAG 3 cut(s) 76, 197, 292
BstDSI CCRYGG 1 cut(s) 26
BstFNI CGCG 1 cut(s) 175
BstMAI GTCTC 1 cut(s) 13
BstMWI GCNNNNNNNGC 1 cut(s) 399
BstNI CCWGG 1 cut(s) 396
BstSCI CCNGG 5 cut(s) 121, 122, 327, 328, 394
BstSFI CTRYAG 1 cut(s) 388
BstSLI GKGCMC 1 cut(s) 155
BstUI CGCG 1 cut(s) 175
BstV1I GCAGC 1 cut(s) 386
BstXI CCANNNNNNTGG 1 cut(s) 33
BsuRI GGCC 2 cut(s) 164, 327
BtgI CCRYGG 1 cut(s) 26
BtsI GCAGTG 1 cut(s) 378
BtsIMutI CAGTG 3 cut(s) 84, 378, 503
Cac8I GCNNGC 1 cut(s) 60
Cfr13I GGNCC 3 cut(s) 118, 326, 379
Cfr9I CCCGGG 2 cut(s) 122, 328
Csp6I GTAC 1 cut(s) 245
CviAII CATG 2 cut(s) 27, 323
CviQI GTAC 1 cut(s) 245
DdeI CTNAG 3 cut(s) 76, 197, 292
DriI GACNNNNNGTC 1 cut(s) 268
Eam1104I CTCTTC 3 cut(s) 201, 475, 516
Eam1105I GACNNNNNGTC 1 cut(s) 268
EarI CTCTTC 3 cut(s) 201, 475, 516
Ecl136II GAGCTC 1 cut(s) 433
Eco130I CCWWGG 1 cut(s) 26
Eco24I GRGCYC 1 cut(s) 435
Eco47I GGWCC 2 cut(s) 118, 379
Eco53kI GAGCTC 1 cut(s) 433
Eco57I CTGAAG 1 cut(s) 497
Eco88I CYCGRG 2 cut(s) 122, 328
EcoICRI GAGCTC 1 cut(s) 433
EcoO109I RGGNCCY 1 cut(s) 118
EcoRI GAATTC 2 cut(s) 82, 464
EcoRII CCWGG 1 cut(s) 394
EcoT14I CCWWGG 1 cut(s) 26
EcoT22I ATGCAT 1 cut(s) 529
EcoT38I GRGCYC 1 cut(s) 435
ErhI CCWWGG 1 cut(s) 26
FaeI CATG 2 cut(s) 30, 326
FaiI YATR 8 cut(s) 28, 113, 231, 324, 369, 489, 491, 529
FaqI GGGAC 4 cut(s) 58, 131, 139, 365
FatI CATG 2 cut(s) 26, 322
Fnu4HI GCNGC 1 cut(s) 400
FriOI GRGCYC 1 cut(s) 435
Fsp4HI GCNGC 1 cut(s) 400
GluI GCNGC 1 cut(s) 400
HaeIII GGCC 2 cut(s) 164, 327
HapII CCGG 2 cut(s) 123, 329
Hin1II CATG 2 cut(s) 30, 326
HinfI GANTC 3 cut(s) 281, 290, 419
HpaII CCGG 2 cut(s) 123, 329
HphI GGTGA 2 cut(s) 162, 272
Hpy188I TCNGA 7 cut(s) 253, 280, 286, 295, 358, 475, 516
Hpy188III TCNNGA 3 cut(s) 337, 436, 448
HpyAV CCTTC 4 cut(s) 22, 46, 150, 207
HpyCH4III ACNGT 2 cut(s) 130, 498
HpyCH4V TGCA 4 cut(s) 371, 402, 480, 527
HpyF10VI GCNNNNNNNGC 1 cut(s) 399
HpyF3I CTNAG 3 cut(s) 76, 197, 292
Hsp92II CATG 2 cut(s) 30, 326
KflI GGGWCCC 1 cut(s) 118
LmnI GCTCC 3 cut(s) 55, 77, 316
Lsp1109I GCAGC 1 cut(s) 386
LweI GCATC 1 cut(s) 514
MaeIII GTNAC 3 cut(s) 168, 260, 341
MboII GAAGA 5 cut(s) 78, 218, 462, 498, 503
MhlI GDGCHC 2 cut(s) 155, 435
MluCI AATT 3 cut(s) 82, 134, 464
MlyI GAGTC 2 cut(s) 284, 290
MmeI TCCRAC 3 cut(s) 258, 294, 447
MnlI CCTC 8 cut(s) 85, 108, 175, 180, 330, 400, 409, 464
Mph1103I ATGCAT 1 cut(s) 529
MseI TTAA 2 cut(s) 147, 183
MslI CAYNNNNRTG 1 cut(s) 158
MspI CCGG 2 cut(s) 123, 329
MspR9I CCNGG 5 cut(s) 123, 124, 329, 330, 396
MvaI CCWGG 1 cut(s) 396
MvnI CGCG 1 cut(s) 175
MwoI GCNNNNNNNGC 1 cut(s) 399
NciI CCSGG 4 cut(s) 123, 124, 329, 330
NcoI CCATGG 1 cut(s) 26
NlaIII CATG 2 cut(s) 30, 326
NlaIV GGNNCC 6 cut(s) 57, 73, 119, 120, 318, 381
NmuCI GTSAC 3 cut(s) 168, 260, 341
NsiI ATGCAT 1 cut(s) 529
PfeI GAWTC 1 cut(s) 419
PflMI CCANNNNNTGG 1 cut(s) 254
PkrI GCNGC 1 cut(s) 401
PleI GAGTC 2 cut(s) 284, 289
PpsI GAGTC 2 cut(s) 284, 289
PpuMI RGGWCCY 1 cut(s) 118
Psp124BI GAGCTC 1 cut(s) 435
Psp5II RGGWCCY 1 cut(s) 118
Psp6I CCWGG 1 cut(s) 394
PspGI CCWGG 1 cut(s) 394
PspN4I GGNNCC 6 cut(s) 57, 73, 119, 120, 318, 381
PspPI GGNCC 3 cut(s) 118, 326, 379
PspPPI RGGWCCY 1 cut(s) 118
RsaI GTAC 1 cut(s) 246
RsaNI GTAC 1 cut(s) 245
RseI CAYNNNNRTG 1 cut(s) 158
SacI GAGCTC 1 cut(s) 435
SaqAI TTAA 2 cut(s) 147, 183
SatI GCNGC 1 cut(s) 400
Sau96I GGNCC 3 cut(s) 118, 326, 379
SchI GAGTC 2 cut(s) 284, 290
ScrFI CCNGG 5 cut(s) 123, 124, 329, 330, 396
SduI GDGCHC 2 cut(s) 155, 435
SetI ASST 4 cut(s) 183, 411, 435, 456
SfaNI GCATC 1 cut(s) 514
SfcI CTRYAG 1 cut(s) 388
SinI GGWCC 2 cut(s) 118, 379
SmaI CCCGGG 2 cut(s) 124, 330
SmiMI CAYNNNNRTG 1 cut(s) 158
SmlI CTYRAG 1 cut(s) 436
SmoI CTYRAG 1 cut(s) 436
Sse9I AATT 3 cut(s) 82, 134, 464
SsiI CCGC 1 cut(s) 173
SstI GAGCTC 1 cut(s) 435
StyD4I CCNGG 5 cut(s) 121, 122, 327, 328, 394
StyI CCWWGG 1 cut(s) 26
TaaI ACNGT 2 cut(s) 130, 498
TaqI TCGA 1 cut(s) 522
TasI AATT 3 cut(s) 82, 134, 464
TfiI GAWTC 1 cut(s) 419
Tru1I TTAA 2 cut(s) 147, 183
Tru9I TTAA 2 cut(s) 147, 183
TscAI CASTG 3 cut(s) 84, 378, 503
TseFI GTSAC 3 cut(s) 168, 260, 341
TseI GCWGC 1 cut(s) 399
Tsp45I GTSAC 3 cut(s) 168, 260, 341
TspDTI ATGAA 1 cut(s) 477
TspGWI ACGGA 1 cut(s) 133
TspMI CCCGGG 2 cut(s) 122, 328
TspRI CASTG 3 cut(s) 84, 378, 503
Van91I CCANNNNNTGG 1 cut(s) 254
VpaK11BI GGWCC 2 cut(s) 118, 379
XapI RAATTY 2 cut(s) 82, 464
XcmI CCANNNNNNNNNTGG 1 cut(s) 33
XmaI CCCGGG 2 cut(s) 122, 328
Zsp2I ATGCAT 1 cut(s) 529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.