MD15G1366300.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0009029
Physical Location & Seq
Forward (+)
44486080 .. 44487042
963 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1366300.v1.1.491

Sequence Viewer

Length: 963 bp
ATGGACGGTTTCGATCGCCTCCCCGACTCGCTCATCCTCCAGATCTTCGACTCGGTCTCCGACATCAAGACCCTCATCCGCTGCCGCGCCGTTTCCAAGCGATTCAACTCGCTCGTCCCCCACACAGACTCGCTCATCCTAACCGTCGACCGCGTCATATCCTCCGATTCAGACTCCGACTCGGAAACAGACGACGTTTCGCACCTCCTCGTCGCCTTCCTCAAATCCATCCTCAAATCGCTCCAGGACCTAGTCTCGGCCAAGCCCGACCCGACCCAGACCCGCTCTCAGAATTCCCCCGCCCAGATCCTCCGCTCCTTCCACCGCGTCCGAAGCCTCTCGATCGAGCTCCCGTCGGGCGATCTCCGGTTAGAGAAGGGGACGGTGCTGAAATGGCGAGCTGAATTCGGGAAAACTCTGAAGACGTGCGTGATCCTAGGGTTCCGATCCGTCGTCGCCAGCGGGGACAATCCTGTGGCCGGAGAGGAAGCGGACTTCGCCGGAGGTCTGAAGGTGAGGGTGGTGTGGACGATTAGCGCGCTGATTGCGGCGTCGGCGAGGCACTACCTGCTGAAGGAGGTGGTGAGGGAGCAGAGAGGGCTGGAATCGCTGGTTCTGAGGGACAGAGAAGGAGAAGGCACGGTGGTGATGAGGAAGGAGGGGCTGAGAGAGTGCAGGAGGGAGGACGCGTGTGGGGAGGCGGAGGAGGAAGAGGGATGGGATCGAGGGAGGAGCAAAGTGCCGAGCGTGAGAATGAGGATGAGGCACGTGGCGAGGGTGGAGCTGAGGGGCGGGGTATGGGTGGAGGGGGCCACACTTGTTGTGGTGAGGCCGAGTTTGGGGGATGACGTGGAGGATGGGGATTTGGCGATGGAGGCGTTTGGGAATGATGGGATGTACGGGGAGGTTGTAGTGGAGCTGCTCAAGGCCAAGAGTTATTTACTGGAAATGAACTCTTTCTAA

Protein Analysis

321

Amino Acids

35.51

Weight (kDa)

5.59

Isoelectric Point (pI)

45.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 4 - 41 1.2e-07 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 576
AccBSI CCGCTC 2 cut(s) 285, 315
AccI GTMKAC 1 cut(s) 147
AccII CGCG 5 cut(s) 87, 153, 327, 539, 689
AclWI GGATC 4 cut(s) 301, 427, 441, 729
AcoI YGGCCR 2 cut(s) 258, 477
AcsI RAATTY 2 cut(s) 292, 404
AcuI CTGAAG 3 cut(s) 440, 530, 593
AcvI CACGTG 1 cut(s) 769
AcyI GRCGYC 1 cut(s) 551
AfaI GTAC 1 cut(s) 899
AfiI CCNNNNNNNGG 4 cut(s) 256, 479, 574, 839
AflIII ACRYGT 1 cut(s) 687
AgsI TTSAA 1 cut(s) 106
AjiI CACGTC 2 cut(s) 426, 850
AjnI CCWGG 1 cut(s) 243
AleI CACNNNNGTG 1 cut(s) 644
AluBI AGCT 4 cut(s) 349, 401, 784, 919
AluI AGCT 4 cut(s) 349, 401, 784, 919
Alw21I GWGCWC 1 cut(s) 351
Alw26I GTCTC 2 cut(s) 61, 259
AlwI GGATC 4 cut(s) 301, 427, 441, 729
AoxI GGCC 5 cut(s) 258, 477, 810, 830, 927
ApeKI GCWGC 2 cut(s) 81, 919
ApoI RAATTY 2 cut(s) 292, 404
Asp700I GAANNNNTTC 1 cut(s) 956
AspA2I CCTAGG 1 cut(s) 436
AspLEI GCGC 3 cut(s) 89, 539, 541
AspS9I GGNCC 2 cut(s) 247, 810
AsuHPI GGTGA 4 cut(s) 526, 595, 658, 838
AvaII GGWCC 1 cut(s) 247
AvrII CCTAGG 1 cut(s) 436
BanII GRGCYC 1 cut(s) 351
BbrPI CACGTG 1 cut(s) 769
BbsI GAAGAC 1 cut(s) 428
Bbv12I GWGCWC 1 cut(s) 351
BbvCI CCTCAGC 1 cut(s) 785
BbvI GCAGC 2 cut(s) 68, 906
BccI CCATC 5 cut(s) 236, 711, 851, 865, 884
BceAI ACGGC 1 cut(s) 74
BciT130I CCWGG 1 cut(s) 245
BcoDI GTCTC 2 cut(s) 61, 259
BfaI CTAG 2 cut(s) 251, 437
BfuAI ACCTGC 1 cut(s) 576
BglII AGATCT 1 cut(s) 42
BisI GCNGC 4 cut(s) 82, 85, 549, 920
BlnI CCTAGG 1 cut(s) 436
BlsI GCNGC 4 cut(s) 83, 86, 550, 921
Bme1390I CCNGG 1 cut(s) 245
Bme18I GGWCC 1 cut(s) 247
BmgBI CACGTC 2 cut(s) 426, 850
BmgT120I GGNCC 2 cut(s) 247, 810
BmiI GGNNCC 2 cut(s) 443, 811
BmrFI CCNGG 1 cut(s) 245
BpiI GAAGAC 1 cut(s) 428
BpmI CTGGAG 2 cut(s) 23, 227
Bpu10I CCTNAGC 1 cut(s) 785
BpuEI CTTGAG 1 cut(s) 908
BsaAI YACGTR 1 cut(s) 769
BsaHI GRCGYC 1 cut(s) 551
BsaI GGTCTC 1 cut(s) 61
BsaJI CCNNGG 1 cut(s) 436
BsaWI WCCGGW 1 cut(s) 366
BsaXI ACNNNNNCTCC 4 cut(s) 41, 71, 674, 704
Bsc4I CCNNNNNNNGG 4 cut(s) 256, 479, 574, 839
Bse1I ACTGG 1 cut(s) 948
BseBI CCWGG 1 cut(s) 245
BseDI CCNNGG 1 cut(s) 436
BseGI GGATG 9 cut(s) 33, 75, 135, 228, 722, 765, 850, 862, 900
BseLI CCNNNNNNNGG 4 cut(s) 256, 479, 574, 839
BseMII CTCAG 4 cut(s) 302, 608, 656, 776
BseNI ACTGG 1 cut(s) 948
BsePI GCGCGC 1 cut(s) 537
BseRI GAGGAG 3 cut(s) 197, 719, 745
BseXI GCAGC 2 cut(s) 68, 906
BsgI GTGCAG 1 cut(s) 694
Bsh1236I CGCG 5 cut(s) 87, 153, 327, 539, 689
Bsh1285I CGRYCG 3 cut(s) 16, 151, 345
BshFI GGCC 5 cut(s) 260, 479, 812, 832, 929
BsiEI CGRYCG 3 cut(s) 16, 151, 345
BsiHKAI GWGCWC 1 cut(s) 351
BsiSI CCGG 3 cut(s) 367, 480, 501
BslFI GGGAC 4 cut(s) 101, 394, 479, 635
BslI CCNNNNNNNGG 4 cut(s) 256, 479, 574, 839
BsmAI GTCTC 2 cut(s) 61, 259
BsmFI GGGAC 4 cut(s) 101, 394, 479, 635
BsnI GGCC 5 cut(s) 260, 479, 812, 832, 929
Bso31I GGTCTC 1 cut(s) 61
Bsp1286I GDGCHC 1 cut(s) 351
Bsp143I GATC 8 cut(s) 13, 42, 306, 342, 361, 432, 446, 721
BspANI GGCC 5 cut(s) 260, 479, 812, 832, 929
BspCNI CTCAG 4 cut(s) 301, 609, 657, 777
BspFNI CGCG 5 cut(s) 87, 153, 327, 539, 689
BspLI GGNNCC 2 cut(s) 443, 811
BspMI ACCTGC 1 cut(s) 576
BspPI GGATC 4 cut(s) 301, 427, 441, 729
BspTNI GGTCTC 1 cut(s) 61
BsrBI CCGCTC 2 cut(s) 285, 315
BsrI ACTGG 1 cut(s) 948
BssECI CCNNGG 1 cut(s) 436
BssHII GCGCGC 1 cut(s) 537
BssMI GATC 8 cut(s) 13, 42, 306, 342, 361, 432, 446, 721
BssNI GRCGYC 1 cut(s) 551
BssT1I CCWWGG 1 cut(s) 436
Bst2UI CCWGG 1 cut(s) 245
Bst4CI ACNGT 4 cut(s) 8, 145, 385, 643
Bst6I CTCTTC 1 cut(s) 705
BstACI GRCGYC 1 cut(s) 551
BstAPI GCANNNNNTGC 1 cut(s) 568
BstBAI YACGTR 1 cut(s) 769
BstC8I GCNNGC 3 cut(s) 399, 460, 539
BstDEI CTNAG 4 cut(s) 288, 617, 665, 785
BstENI CCTNNNNNAGG 1 cut(s) 572
BstF5I GGATG 9 cut(s) 33, 75, 135, 228, 722, 765, 850, 862, 900
BstFNI CGCG 5 cut(s) 87, 153, 327, 539, 689
BstHHI GCGC 3 cut(s) 89, 539, 541
BstKTI GATC 8 cut(s) 16, 45, 309, 345, 364, 435, 449, 724
BstMAI GTCTC 2 cut(s) 61, 259
BstMBI GATC 8 cut(s) 13, 42, 306, 342, 361, 432, 446, 721
BstMCI CGRYCG 3 cut(s) 16, 151, 345
BstMWI GCNNNNNNNGC 9 cut(s) 333, 394, 497, 545, 554, 568, 598, 607, 875
BstNI CCWGG 1 cut(s) 245
BstSCI CCNGG 1 cut(s) 243
BstUI CGCG 5 cut(s) 87, 153, 327, 539, 689
BstV1I GCAGC 2 cut(s) 68, 906
BstV2I GAAGAC 1 cut(s) 428
BstX2I RGATCY 2 cut(s) 42, 306
BstYI RGATCY 2 cut(s) 42, 306
BsuRI GGCC 5 cut(s) 260, 479, 812, 832, 929
BtgZI GCGATG 1 cut(s) 884
BtrI CACGTC 2 cut(s) 426, 850
BtsCI GGATG 9 cut(s) 33, 75, 135, 228, 722, 765, 850, 862, 900
BveI ACCTGC 1 cut(s) 576
Cac8I GCNNGC 3 cut(s) 399, 460, 539
CfoI GCGC 3 cut(s) 89, 539, 541
Cfr13I GGNCC 2 cut(s) 247, 810
CseI GACGC 4 cut(s) 142, 316, 540, 695
Csp6I GTAC 1 cut(s) 898
CspCI CAANNNNNGTGG 2 cut(s) 802, 837
CviQI GTAC 1 cut(s) 898
DdeI CTNAG 4 cut(s) 288, 617, 665, 785
DpnI GATC 8 cut(s) 15, 44, 308, 344, 363, 434, 448, 723
DpnII GATC 8 cut(s) 13, 42, 306, 342, 361, 432, 446, 721
EaeI YGGCCR 2 cut(s) 258, 477
Eam1104I CTCTTC 1 cut(s) 705
EarI CTCTTC 1 cut(s) 705
EciI GGCGGA 1 cut(s) 716
Ecl136II GAGCTC 1 cut(s) 349
Eco130I CCWWGG 1 cut(s) 436
Eco24I GRGCYC 1 cut(s) 351
Eco31I GGTCTC 1 cut(s) 61
Eco47I GGWCC 1 cut(s) 247
Eco53kI GAGCTC 1 cut(s) 349
Eco57I CTGAAG 3 cut(s) 440, 530, 593
Eco72I CACGTG 1 cut(s) 769
EcoICRI GAGCTC 1 cut(s) 349
EcoNI CCTNNNNNAGG 1 cut(s) 572
EcoO109I RGGNCCY 1 cut(s) 247
EcoRI GAATTC 2 cut(s) 292, 404
EcoRII CCWGG 1 cut(s) 243
EcoT14I CCWWGG 1 cut(s) 436
EcoT38I GRGCYC 1 cut(s) 351
ErhI CCWWGG 1 cut(s) 436
FaiI YATR 2 cut(s) 158, 799
FaqI GGGAC 4 cut(s) 101, 394, 479, 635
FauI CCCGC 4 cut(s) 290, 307, 455, 785
FblI GTMKAC 1 cut(s) 147
Fnu4HI GCNGC 4 cut(s) 82, 85, 549, 920
FokI GGATG 9 cut(s) 20, 62, 122, 215, 729, 772, 857, 869, 907
FriOI GRGCYC 1 cut(s) 351
Fsp4HI GCNGC 4 cut(s) 82, 85, 549, 920
FspBI CTAG 2 cut(s) 251, 437
GlaI GCGC 3 cut(s) 88, 538, 540
GluI GCNGC 4 cut(s) 82, 85, 549, 920
GsuI CTGGAG 2 cut(s) 23, 227
HaeIII GGCC 5 cut(s) 260, 479, 812, 832, 929
HapII CCGG 3 cut(s) 367, 480, 501
HgaI GACGC 4 cut(s) 142, 316, 540, 695
HhaI GCGC 3 cut(s) 89, 539, 541
Hin1I GRCGYC 1 cut(s) 551
Hin6I GCGC 3 cut(s) 87, 537, 539
HinP1I GCGC 3 cut(s) 87, 537, 539
HincII GTYRAC 1 cut(s) 148
HindII GTYRAC 1 cut(s) 148
HinfI GANTC 8 cut(s) 26, 50, 102, 128, 167, 173, 179, 605
HpaII CCGG 3 cut(s) 367, 480, 501
HphI GGTGA 4 cut(s) 526, 595, 658, 838
Hpy166II GTNNAC 2 cut(s) 148, 528
Hpy188III TCNNGA 4 cut(s) 40, 67, 340, 409
Hpy8I GTNNAC 2 cut(s) 148, 528
Hpy99I CGWCG 7 cut(s) 149, 197, 215, 358, 455, 458, 556
HpyAV CCTTC 8 cut(s) 226, 328, 370, 505, 568, 623, 629, 649
HpyCH4III ACNGT 4 cut(s) 8, 145, 385, 643
HpyCH4IV ACGT 4 cut(s) 195, 425, 768, 849
HpyCH4V TGCA 1 cut(s) 675
HpyF10VI GCNNNNNNNGC 9 cut(s) 333, 394, 497, 545, 554, 568, 598, 607, 875
HpyF3I CTNAG 4 cut(s) 288, 617, 665, 785
HpySE526I ACGT 4 cut(s) 195, 425, 768, 849
Hsp92I GRCGYC 1 cut(s) 551
HspAI GCGC 3 cut(s) 87, 537, 539
Kzo9I GATC 8 cut(s) 13, 42, 306, 342, 361, 432, 446, 721
LmnI GCTCC 7 cut(s) 246, 320, 354, 589, 732, 781, 916
Lsp1109I GCAGC 2 cut(s) 68, 906
MaeI CTAG 2 cut(s) 251, 437
MaeII ACGT 4 cut(s) 195, 425, 768, 849
MalI GATC 8 cut(s) 15, 44, 308, 344, 363, 434, 448, 723
MbiI CCGCTC 2 cut(s) 285, 315
MboI GATC 8 cut(s) 13, 42, 306, 342, 361, 432, 446, 721
MboII GAAGA 3 cut(s) 37, 433, 722
MflI RGATCY 2 cut(s) 42, 306
MhlI GDGCHC 1 cut(s) 351
MluCI AATT 2 cut(s) 292, 404
MluI ACGCGT 1 cut(s) 687
MlyI GAGTC 5 cut(s) 20, 44, 122, 167, 173
MmeI TCCRAC 2 cut(s) 84, 201
MroXI GAANNNNTTC 1 cut(s) 956
MslI CAYNNNNRTG 1 cut(s) 644
MspA1I CMGCKG 2 cut(s) 81, 462
MspI CCGG 3 cut(s) 367, 480, 501
MspR9I CCNGG 1 cut(s) 245
MvaI CCWGG 1 cut(s) 245
MvnI CGCG 5 cut(s) 87, 153, 327, 539, 689
MwoI GCNNNNNNNGC 9 cut(s) 333, 394, 497, 545, 554, 568, 598, 607, 875
NdeII GATC 8 cut(s) 13, 42, 306, 342, 361, 432, 446, 721
NlaIV GGNNCC 2 cut(s) 443, 811
NmeAIII GCCGAG 3 cut(s) 236, 768, 858
OliI CACNNNNGTG 1 cut(s) 644
PauI GCGCGC 1 cut(s) 537
PdmI GAANNNNTTC 1 cut(s) 956
PfeI GAWTC 3 cut(s) 102, 167, 605
PflFI GACNNNGTC 3 cut(s) 53, 152, 251
PfoI TCCNGGA 1 cut(s) 243
PkrI GCNGC 4 cut(s) 83, 86, 550, 921
Ple19I CGATCG 2 cut(s) 16, 345
PleI GAGTC 5 cut(s) 20, 44, 122, 167, 173
PmaCI CACGTG 1 cut(s) 769
PmlI CACGTG 1 cut(s) 769
PpsI GAGTC 5 cut(s) 20, 44, 122, 167, 173
Ppu21I YACGTR 1 cut(s) 769
PpuMI RGGWCCY 1 cut(s) 247
Psp124BI GAGCTC 1 cut(s) 351
Psp5II RGGWCCY 1 cut(s) 247
Psp6I CCWGG 1 cut(s) 243
PspCI CACGTG 1 cut(s) 769
PspGI CCWGG 1 cut(s) 243
PspN4I GGNNCC 2 cut(s) 443, 811
PspPI GGNCC 2 cut(s) 247, 810
PspPPI RGGWCCY 1 cut(s) 247
PsuI RGATCY 2 cut(s) 42, 306
PsyI GACNNNGTC 3 cut(s) 53, 152, 251
PteI GCGCGC 1 cut(s) 537
PvuI CGATCG 2 cut(s) 16, 345
RsaI GTAC 1 cut(s) 899
RsaNI GTAC 1 cut(s) 898
RseI CAYNNNNRTG 1 cut(s) 644
SacI GAGCTC 1 cut(s) 351
SalI GTCGAC 1 cut(s) 146
SatI GCNGC 4 cut(s) 82, 85, 549, 920
Sau3AI GATC 8 cut(s) 13, 42, 306, 342, 361, 432, 446, 721
Sau96I GGNCC 2 cut(s) 247, 810
SchI GAGTC 5 cut(s) 20, 44, 122, 167, 173
ScrFI CCNGG 1 cut(s) 245
SduI GDGCHC 1 cut(s) 351
SinI GGWCC 1 cut(s) 247
SmiMI CAYNNNNRTG 1 cut(s) 644
SmlI CTYRAG 1 cut(s) 923
SmoI CTYRAG 1 cut(s) 923
Sse9I AATT 2 cut(s) 292, 404
SspMI CTAG 2 cut(s) 251, 437
SstI GAGCTC 1 cut(s) 351
StyD4I CCNGG 1 cut(s) 243
StyI CCWWGG 1 cut(s) 436
TaaI ACNGT 4 cut(s) 8, 145, 385, 643
TaiI ACGT 4 cut(s) 198, 428, 771, 852
TaqI TCGA 6 cut(s) 12, 48, 147, 341, 345, 724
TaqII GACCGA 1 cut(s) 43
TasI AATT 2 cut(s) 292, 404
TauI GCSGC 2 cut(s) 87, 551
TfiI GAWTC 3 cut(s) 102, 167, 605
TseI GCWGC 2 cut(s) 81, 919
TspGWI ACGGA 1 cut(s) 439
Tth111I GACNNNGTC 3 cut(s) 53, 152, 251
VpaK11BI GGWCC 1 cut(s) 247
XagI CCTNNNNNAGG 1 cut(s) 572
XapI RAATTY 2 cut(s) 292, 404
XcmI CCANNNNNNNNNTGG 1 cut(s) 820
XmaJI CCTAGG 1 cut(s) 436
XmiI GTMKAC 1 cut(s) 147
XmnI GAANNNNTTC 1 cut(s) 956
XspI CTAG 2 cut(s) 251, 437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.