Rw7G040370

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0009029
Physical Location & Seq
Reverse (-)
62663714 .. 62665277
1564 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G040370.1

Sequence Viewer

Length: 993 bp
ATGGAAATGGACGGCTTCGATCGGCTCCCGGACCCGGTGATCCTCCAGATCTTCGACCTGGTCTCGGACACCAAGACCCTCATTCGCTGCCTCGCAGTTTCGAAACGCTTCAACTCCCTCGTCCCCCACACCGACTCCCTAGTCCTCACCGTCGACCGCGTCATCTCCTCCGACTCCGACTCCGACTCAGACGACGACGTTTCCAACCTCCTCGCCTCCGCCTTCCGCTACATCCTCAAGTCTCTCCACAGCCTCGTCTGCCCCAAGCCCAACAACAACAACAACAACAGCCCGACCCGGACCCGGATCCCCAACTGCCCCGCCCAGATCCTCCGCTGCTTCCACCGCGTCAAGCGCCTCGCGATCGACCTCCCCTCCGGCGACCTCCGCCTCGAGAAGGGGACCGTTCTCAAGTGGAGAGCCGAGTTCGGGAAAACCCTCCGGACCTGCGTCATCCTAGGGTTCCGATCCGTCGGGGAGACGTCGTCGCCGGTGACGGCCGGAGCTGAGCCCGACTTAACCGGCGGGCTGAAGGTGCGGGTGGTGTGGACGATCAGTGCTCTGATCGCGGCGTCGGCGAGGCACTATTTGTTGAAGGAGGTGGTGAGGGAGCAGAGCGGGCTCGAATGTCTGGTTCTGAGGGACAGAGAGGCGGAGGGGACGGTGGTGATGGAGAAGGAGGGGTTGAGGGAGTGTAGAGAAGAAGGCGAGGAGTTTGCTGATGAGGAGGAGGGGTTGGATAGGTGCAGGACTAGAGTGCCTAGTGTGAGGATGAGGATGAGGCATGTGCCGAGATTGGAGCTCAAAGGCGGGCTTTCGGTCGAGGGTGCGACGCTCGTGGTGGTCCGGCCCAGTTTGGGAGGCGGCAGTGGCGACATGGCGGTGGAGGATGGGGAATTGGCGTTGAGGGCATTTGGGAATGGTTTGTACGGGGAGGTTGCGCAGAAGCTGCTCAAGGCCAGAAGCTATTTGCTGGAAATGAACTCGTTCTGA

Protein Analysis

330

Amino Acids

36.48

Weight (kDa)

6.28

Isoelectric Point (pI)

40.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 6 - 43 1.1e-07 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 140
AatII GACGTC 1 cut(s) 485
Acc16I TGCGCA 1 cut(s) 942
Acc36I ACCTGC 1 cut(s) 455
AccBSI CCGCTC 1 cut(s) 618
AccI GTMKAC 1 cut(s) 153
AccII CGCG 4 cut(s) 159, 348, 362, 569
AccIII TCCGGA 1 cut(s) 441
AclWI GGATC 5 cut(s) 34, 301, 314, 322, 462
AcoI YGGCCR 1 cut(s) 498
AcuI CTGAAG 1 cut(s) 551
AcyI GRCGYC 2 cut(s) 482, 572
AfaI GTAC 1 cut(s) 929
AfiI CCNNNNNNNGG 6 cut(s) 34, 64, 303, 397, 429, 857
AgsI TTSAA 2 cut(s) 112, 595
AjnI CCWGG 1 cut(s) 57
AluBI AGCT 4 cut(s) 506, 802, 949, 966
AluI AGCT 4 cut(s) 506, 802, 949, 966
Alw21I GWGCWC 2 cut(s) 562, 804
Alw26I GTCTC 3 cut(s) 67, 246, 473
AlwI GGATC 5 cut(s) 34, 301, 314, 322, 462
AlwNI CAGNNNCTG 1 cut(s) 949
Ama87I CYCGRG 1 cut(s) 392
Aor13HI TCCGGA 1 cut(s) 441
AoxI GGCC 3 cut(s) 498, 848, 957
ApeKI GCWGC 3 cut(s) 87, 336, 949
Asp700I GAANNNNTTC 2 cut(s) 107, 986
AspA2I CCTAGG 1 cut(s) 457
AspLEI GCGC 2 cut(s) 357, 943
AspS9I GGNCC 6 cut(s) 31, 300, 402, 444, 844, 849
AsuC2I CCSGG 4 cut(s) 29, 35, 298, 304
AsuHPI GGTGA 5 cut(s) 49, 139, 505, 616, 679
AsuII TTCGAA 1 cut(s) 101
AvaI CYCGRG 1 cut(s) 392
AvaII GGWCC 5 cut(s) 31, 300, 402, 444, 844
AvrII CCTAGG 1 cut(s) 457
BamHI GGATCC 1 cut(s) 306
BanII GRGCYC 3 cut(s) 513, 624, 804
BauI CACGAG 1 cut(s) 836
Bbv12I GWGCWC 2 cut(s) 562, 804
BbvI GCAGC 3 cut(s) 74, 323, 936
BccI CCATC 2 cut(s) 664, 884
BceAI ACGGC 2 cut(s) 28, 513
BcgI CGANNNNNNTGC 2 cut(s) 698, 732
BciT130I CCWGG 1 cut(s) 59
BcnI CCSGG 4 cut(s) 29, 35, 298, 304
BcoDI GTCTC 3 cut(s) 67, 246, 473
BfaI CTAG 4 cut(s) 140, 458, 753, 762
BfoI RGCGCY 1 cut(s) 358
BfuAI ACCTGC 1 cut(s) 455
BglII AGATCT 1 cut(s) 48
BisI GCNGC 5 cut(s) 88, 337, 570, 865, 950
BlnI CCTAGG 1 cut(s) 457
BlpI GCTNAGC 1 cut(s) 507
BlsI GCNGC 5 cut(s) 89, 338, 571, 866, 951
Bme1390I CCNGG 5 cut(s) 29, 35, 59, 298, 304
Bme18I GGWCC 5 cut(s) 31, 300, 402, 444, 844
BmeT110I CYCGRG 1 cut(s) 392
BmgT120I GGNCC 6 cut(s) 31, 300, 402, 444, 844, 849
BmiI GGNNCC 6 cut(s) 26, 33, 302, 308, 403, 464
BmrFI CCNGG 5 cut(s) 29, 35, 59, 298, 304
BmrI ACTGGG 1 cut(s) 846
BmuI ACTGGG 1 cut(s) 846
BoxI GACNNNNGTC 1 cut(s) 449
BpmI CTGGAG 1 cut(s) 29
Bpu1102I GCTNAGC 1 cut(s) 507
Bpu14I TTCGAA 1 cut(s) 101
BpuEI CTTGAG 3 cut(s) 221, 395, 938
BpuMI CCSGG 4 cut(s) 29, 35, 298, 304
BsaHI GRCGYC 2 cut(s) 482, 572
BsaI GGTCTC 1 cut(s) 67
BsaJI CCNNGG 1 cut(s) 457
BsaWI WCCGGW 1 cut(s) 441
Bsc4I CCNNNNNNNGG 6 cut(s) 34, 64, 303, 397, 429, 857
Bse118I RCCGGY 2 cut(s) 490, 521
Bse1I ACTGG 1 cut(s) 852
BseAI TCCGGA 1 cut(s) 441
BseBI CCWGG 1 cut(s) 59
BseDI CCNNGG 1 cut(s) 457
BseGI GGATG 5 cut(s) 231, 453, 777, 783, 895
BseLI CCNNNNNNNGG 6 cut(s) 34, 64, 303, 397, 429, 857
BseMII CTCAG 3 cut(s) 201, 498, 629
BseNI ACTGG 1 cut(s) 852
BseRI GAGGAG 5 cut(s) 157, 200, 725, 740, 743
BseX3I CGGCCG 1 cut(s) 498
BseXI GCAGC 3 cut(s) 74, 323, 936
BsgI GTGCAG 1 cut(s) 766
Bsh1236I CGCG 4 cut(s) 159, 348, 362, 569
Bsh1285I CGRYCG 5 cut(s) 22, 157, 366, 501, 822
BshFI GGCC 3 cut(s) 500, 850, 959
BsiEI CGRYCG 5 cut(s) 22, 157, 366, 501, 822
BsiHKAI GWGCWC 2 cut(s) 562, 804
BsiHKCI CYCGRG 1 cut(s) 392
BslFI GGGAC 4 cut(s) 107, 415, 656, 673
BslI CCNNNNNNNGG 6 cut(s) 34, 64, 303, 397, 429, 857
BsmAI GTCTC 3 cut(s) 67, 246, 473
BsmBI CGTCTC 1 cut(s) 473
BsmFI GGGAC 4 cut(s) 107, 415, 656, 673
BsnI GGCC 3 cut(s) 500, 850, 959
Bso31I GGTCTC 1 cut(s) 67
BsoBI CYCGRG 1 cut(s) 392
Bsp119I TTCGAA 1 cut(s) 101
Bsp1286I GDGCHC 4 cut(s) 513, 562, 624, 804
Bsp13I TCCGGA 1 cut(s) 441
Bsp143I GATC 9 cut(s) 19, 39, 48, 306, 327, 363, 467, 552, 564
Bsp1720I GCTNAGC 1 cut(s) 507
Bsp68I TCGCGA 1 cut(s) 362
BspANI GGCC 3 cut(s) 500, 850, 959
BspCNI CTCAG 3 cut(s) 200, 499, 630
BspEI TCCGGA 1 cut(s) 441
BspFNI CGCG 4 cut(s) 159, 348, 362, 569
BspLI GGNNCC 6 cut(s) 26, 33, 302, 308, 403, 464
BspMI ACCTGC 1 cut(s) 455
BspPI GGATC 5 cut(s) 34, 301, 314, 322, 462
BspT104I TTCGAA 1 cut(s) 101
BspTNI GGTCTC 1 cut(s) 67
BsrBI CCGCTC 1 cut(s) 618
BsrFI RCCGGY 2 cut(s) 490, 521
BsrI ACTGG 1 cut(s) 852
BssAI RCCGGY 2 cut(s) 490, 521
BssECI CCNNGG 1 cut(s) 457
BssMI GATC 9 cut(s) 19, 39, 48, 306, 327, 363, 467, 552, 564
BssNI GRCGYC 2 cut(s) 482, 572
BssSI CACGAG 1 cut(s) 836
BssT1I CCWWGG 1 cut(s) 457
Bst2BI CACGAG 1 cut(s) 836
Bst2UI CCWGG 1 cut(s) 59
Bst4CI ACNGT 3 cut(s) 151, 406, 664
BstACI GRCGYC 2 cut(s) 482, 572
BstAPI GCANNNNNTGC 1 cut(s) 949
BstBI TTCGAA 1 cut(s) 101
BstC8I GCNNGC 3 cut(s) 527, 620, 812
BstDEI CTNAG 3 cut(s) 187, 507, 638
BstENI CCTNNNNNAGG 1 cut(s) 395
BstF5I GGATG 5 cut(s) 231, 453, 777, 783, 895
BstFNI CGCG 4 cut(s) 159, 348, 362, 569
BstH2I RGCGCY 1 cut(s) 358
BstHHI GCGC 2 cut(s) 357, 943
BstKTI GATC 9 cut(s) 22, 42, 51, 309, 330, 366, 470, 555, 567
BstMAI GTCTC 3 cut(s) 67, 246, 473
BstMBI GATC 9 cut(s) 19, 39, 48, 306, 327, 363, 467, 552, 564
BstMCI CGRYCG 5 cut(s) 22, 157, 366, 501, 822
BstNI CCWGG 1 cut(s) 59
BstNSI RCATGY 1 cut(s) 788
BstPAI GACNNNNGTC 1 cut(s) 449
BstSCI CCNGG 5 cut(s) 27, 33, 57, 296, 302
BstUI CGCG 4 cut(s) 159, 348, 362, 569
BstV1I GCAGC 3 cut(s) 74, 323, 936
BstX2I RGATCY 3 cut(s) 48, 306, 327
BstYI RGATCY 3 cut(s) 48, 306, 327
BstZI CGGCCG 1 cut(s) 498
BsuRI GGCC 3 cut(s) 500, 850, 959
BtsCI GGATG 5 cut(s) 231, 453, 777, 783, 895
BtsI GCAGTG 1 cut(s) 874
BtsIMutI CAGTG 2 cut(s) 562, 874
BtuMI TCGCGA 1 cut(s) 362
BveI ACCTGC 1 cut(s) 455
Cac8I GCNNGC 3 cut(s) 527, 620, 812
CaiI CAGNNNCTG 1 cut(s) 949
CfoI GCGC 2 cut(s) 357, 943
Cfr10I RCCGGY 2 cut(s) 490, 521
Cfr13I GGNCC 6 cut(s) 31, 300, 402, 444, 844, 849
CseI GACGC 5 cut(s) 148, 337, 439, 561, 841
CsiI ACCWGGT 1 cut(s) 57
Csp6I GTAC 1 cut(s) 928
CviAII CATG 2 cut(s) 785, 877
CviQI GTAC 1 cut(s) 928
DdeI CTNAG 3 cut(s) 187, 507, 638
DpnI GATC 9 cut(s) 21, 41, 50, 308, 329, 365, 469, 554, 566
DpnII GATC 9 cut(s) 19, 39, 48, 306, 327, 363, 467, 552, 564
DrdI GACNNNNNNGTC 1 cut(s) 140
DseDI GACNNNNNNGTC 1 cut(s) 140
EaeI YGGCCR 1 cut(s) 498
EagI CGGCCG 1 cut(s) 498
EciI GGCGGA 3 cut(s) 208, 377, 668
Ecl136II GAGCTC 1 cut(s) 802
EclXI CGGCCG 1 cut(s) 498
Eco130I CCWWGG 1 cut(s) 457
Eco24I GRGCYC 3 cut(s) 513, 624, 804
Eco31I GGTCTC 1 cut(s) 67
Eco47I GGWCC 5 cut(s) 31, 300, 402, 444, 844
Eco52I CGGCCG 1 cut(s) 498
Eco53kI GAGCTC 1 cut(s) 802
Eco57I CTGAAG 1 cut(s) 551
Eco88I CYCGRG 1 cut(s) 392
EcoICRI GAGCTC 1 cut(s) 802
EcoNI CCTNNNNNAGG 1 cut(s) 395
EcoRII CCWGG 1 cut(s) 57
EcoT14I CCWWGG 1 cut(s) 457
EcoT38I GRGCYC 3 cut(s) 513, 624, 804
ErhI CCWWGG 1 cut(s) 457
Esp3I CGTCTC 1 cut(s) 473
FaeI CATG 2 cut(s) 788, 880
FaiI YATR 2 cut(s) 786, 878
FalI AAGNNNNNCTT 2 cut(s) 798, 830
FaqI GGGAC 4 cut(s) 107, 415, 656, 673
FatI CATG 2 cut(s) 784, 876
FauI CCCGC 5 cut(s) 328, 518, 531, 611, 803
FblI GTMKAC 1 cut(s) 153
Fnu4HI GCNGC 5 cut(s) 88, 337, 570, 865, 950
FokI GGATG 5 cut(s) 218, 440, 784, 790, 902
FriOI GRGCYC 3 cut(s) 513, 624, 804
Fsp4HI GCNGC 5 cut(s) 88, 337, 570, 865, 950
FspBI CTAG 4 cut(s) 140, 458, 753, 762
FspI TGCGCA 1 cut(s) 942
GlaI GCGC 2 cut(s) 356, 942
GluI GCNGC 5 cut(s) 88, 337, 570, 865, 950
GsuI CTGGAG 1 cut(s) 29
HaeII RGCGCY 1 cut(s) 358
HaeIII GGCC 3 cut(s) 500, 850, 959
HgaI GACGC 5 cut(s) 148, 337, 439, 561, 841
HhaI GCGC 2 cut(s) 357, 943
Hin1I GRCGYC 2 cut(s) 482, 572
Hin1II CATG 2 cut(s) 788, 880
Hin6I GCGC 2 cut(s) 355, 941
HinP1I GCGC 2 cut(s) 355, 941
HincII GTYRAC 1 cut(s) 154
HindII GTYRAC 1 cut(s) 154
HinfI GANTC 4 cut(s) 134, 173, 179, 185
HphI GGTGA 5 cut(s) 49, 139, 505, 616, 679
Hpy166II GTNNAC 2 cut(s) 154, 549
Hpy188I TCNGA 9 cut(s) 67, 172, 178, 184, 190, 467, 564, 639, 992
Hpy188III TCNNGA 5 cut(s) 46, 361, 394, 430, 442
Hpy8I GTNNAC 2 cut(s) 154, 549
Hpy99I CGWCG 8 cut(s) 155, 197, 200, 476, 487, 490, 577, 835
HpyAV CCTTC 6 cut(s) 232, 391, 526, 589, 670, 698
HpyCH4III ACNGT 3 cut(s) 151, 406, 664
HpyCH4IV ACGT 2 cut(s) 198, 482
HpyCH4V TGCA 1 cut(s) 747
HpyF3I CTNAG 3 cut(s) 187, 507, 638
HpySE526I ACGT 2 cut(s) 198, 482
Hsp92I GRCGYC 2 cut(s) 482, 572
Hsp92II CATG 2 cut(s) 788, 880
HspAI GCGC 2 cut(s) 355, 941
Kpn2I TCCGGA 1 cut(s) 441
Kzo9I GATC 9 cut(s) 19, 39, 48, 306, 327, 363, 467, 552, 564
LmnI GCTCC 4 cut(s) 30, 503, 610, 799
Lsp1109I GCAGC 3 cut(s) 74, 323, 936
MabI ACCWGGT 1 cut(s) 57
MaeI CTAG 4 cut(s) 140, 458, 753, 762
MaeII ACGT 2 cut(s) 198, 482
MaeIII GTNAC 1 cut(s) 493
MalI GATC 9 cut(s) 21, 41, 50, 308, 329, 365, 469, 554, 566
MbiI CCGCTC 1 cut(s) 618
MboI GATC 9 cut(s) 19, 39, 48, 306, 327, 363, 467, 552, 564
MboII GAAGA 2 cut(s) 43, 713
MflI RGATCY 3 cut(s) 48, 306, 327
MhlI GDGCHC 4 cut(s) 513, 562, 624, 804
MluCI AATT 1 cut(s) 896
MlyI GAGTC 4 cut(s) 128, 167, 173, 179
MmeI TCCRAC 5 cut(s) 195, 201, 207, 228, 717
MroI TCCGGA 1 cut(s) 441
MroXI GAANNNNTTC 2 cut(s) 107, 986
MseI TTAA 1 cut(s) 518
MslI CAYNNNNRTG 1 cut(s) 881
MspA1I CMGCKG 1 cut(s) 336
MspR9I CCNGG 5 cut(s) 29, 35, 59, 298, 304
MvaI CCWGG 1 cut(s) 59
MvnI CGCG 4 cut(s) 159, 348, 362, 569
NciI CCSGG 4 cut(s) 29, 35, 298, 304
NdeII GATC 9 cut(s) 19, 39, 48, 306, 327, 363, 467, 552, 564
NlaIII CATG 2 cut(s) 788, 880
NlaIV GGNNCC 6 cut(s) 26, 33, 302, 308, 403, 464
NmeAIII GCCGAG 2 cut(s) 448, 816
NmuCI GTSAC 1 cut(s) 493
NruI TCGCGA 1 cut(s) 362
NsbI TGCGCA 1 cut(s) 942
NspI RCATGY 1 cut(s) 788
NspV TTCGAA 1 cut(s) 101
PaeR7I CTCGAG 1 cut(s) 392
PdmI GAANNNNTTC 2 cut(s) 107, 986
PflFI GACNNNGTC 3 cut(s) 59, 158, 484
PfoI TCCNGGA 1 cut(s) 27
PkrI GCNGC 5 cut(s) 89, 338, 571, 866, 951
Ple19I CGATCG 2 cut(s) 22, 366
PleI GAGTC 4 cut(s) 128, 167, 173, 179
PpsI GAGTC 4 cut(s) 128, 167, 173, 179
PshAI GACNNNNGTC 1 cut(s) 449
Psp124BI GAGCTC 1 cut(s) 804
Psp6I CCWGG 1 cut(s) 57
PspGI CCWGG 1 cut(s) 57
PspN4I GGNNCC 6 cut(s) 26, 33, 302, 308, 403, 464
PspPI GGNCC 6 cut(s) 31, 300, 402, 444, 844, 849
PstNI CAGNNNCTG 1 cut(s) 949
PsuI RGATCY 3 cut(s) 48, 306, 327
PsyI GACNNNGTC 3 cut(s) 59, 158, 484
PvuI CGATCG 2 cut(s) 22, 366
RruI TCGCGA 1 cut(s) 362
RsaI GTAC 1 cut(s) 929
RsaNI GTAC 1 cut(s) 928
RseI CAYNNNNRTG 1 cut(s) 881
SacI GAGCTC 1 cut(s) 804
SalI GTCGAC 1 cut(s) 152
SaqAI TTAA 1 cut(s) 518
SatI GCNGC 5 cut(s) 88, 337, 570, 865, 950
Sau3AI GATC 9 cut(s) 19, 39, 48, 306, 327, 363, 467, 552, 564
Sau96I GGNCC 6 cut(s) 31, 300, 402, 444, 844, 849
SchI GAGTC 4 cut(s) 128, 167, 173, 179
ScrFI CCNGG 5 cut(s) 29, 35, 59, 298, 304
SduI GDGCHC 4 cut(s) 513, 562, 624, 804
SexAI ACCWGGT 1 cut(s) 57
Sfr274I CTCGAG 1 cut(s) 392
SfuI TTCGAA 1 cut(s) 101
SgrAI CRCCGGYG 1 cut(s) 490
SinI GGWCC 5 cut(s) 31, 300, 402, 444, 844
SlaI CTCGAG 1 cut(s) 392
SmiMI CAYNNNNRTG 1 cut(s) 881
SmlI CTYRAG 4 cut(s) 236, 392, 410, 953
SmoI CTYRAG 4 cut(s) 236, 392, 410, 953
Sse9I AATT 1 cut(s) 896
SspMI CTAG 4 cut(s) 140, 458, 753, 762
SstI GAGCTC 1 cut(s) 804
StyD4I CCNGG 5 cut(s) 27, 33, 57, 296, 302
StyI CCWWGG 1 cut(s) 457
TaaI ACNGT 3 cut(s) 151, 406, 664
TaiI ACGT 2 cut(s) 201, 485
TaqI TCGA 8 cut(s) 18, 54, 101, 153, 366, 393, 624, 822
TaqII GACCGA 1 cut(s) 808
TasI AATT 1 cut(s) 896
TauI GCSGC 2 cut(s) 572, 867
Tru1I TTAA 1 cut(s) 518
Tru9I TTAA 1 cut(s) 518
TscAI CASTG 2 cut(s) 562, 874
TseFI GTSAC 1 cut(s) 493
TseI GCWGC 3 cut(s) 87, 336, 949
Tsp45I GTSAC 1 cut(s) 493
TspGWI ACGGA 1 cut(s) 460
TspRI CASTG 2 cut(s) 562, 874
Tth111I GACNNNGTC 3 cut(s) 59, 158, 484
VpaK11BI GGWCC 5 cut(s) 31, 300, 402, 444, 844
XagI CCTNNNNNAGG 1 cut(s) 395
XceI RCATGY 1 cut(s) 788
XhoI CTCGAG 1 cut(s) 392
XmaJI CCTAGG 1 cut(s) 457
XmiI GTMKAC 1 cut(s) 153
XmnI GAANNNNTTC 2 cut(s) 107, 986
XspI CTAG 4 cut(s) 140, 458, 753, 762
ZraI GACGTC 1 cut(s) 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.