pycom15g32850

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009029
Physical Location & Seq
Forward (+)
32109373 .. 32110344
972 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g32850.1

Sequence Viewer

Length: 972 bp
ATGGACGGTTTCGATCGCCTCCCCGACTCGCTCATCCTCCAGATCTTCGACTCGGTCTCCGACATCAAGACCCTCATACGCTGCCGCGCCGTTTCCAAGCGATTCAACTCGCTCGTCCCCCACACAGACTCGCTCATCCTAACCGTCGACCGCGTCATATCCTCCGATTCAGACTCCGACTCGGAAAGCGACGACGTTTCGCACCTCCTCGTCGCCTTCCTCAAATCAATCCTCAAATCGCTCCAGGACCTAGTCTCGGCCAAGCCCGACCCGACCCAGACCCGCTCTCAGAATTCCCCCGCCCAGATCCTCCGCTCCTTCCACCGCGTCCGAAGCCTCTCGATCGAGCTCCCGTCGGGCGATCTCCGGTTAGAGAAGGGGACGGTGCTGAAATGGCGAGCTGAATTCGGGAAAACTCTGAAGACGTGCGTGATCCTAGGGTTCCGATCCGTCGTCGCCAGCGGGGACAATCCTGTGGCCGGAGAGGAAGCGGACTTCGCCGGAGGTCTGAAGGTGAGGGTGGTGTGGACGATTAGCGCGCTGATCGCGGCGTCGGCGAGGCACTACCTGCTGAAGGAGGTGGTGAGGGAGCAGAGAGGGCTGGAATCGCTGGTTCTGAGGGACAGAGAAGGAGAAGGCACGGTGGTGATGAGGAAGGAGGGGCTGAGAGAGTGCAGGAGGGAGGACGCGTGTGGGGAGGCGGAGGAGGTGGAGGAGGAAGAGGGATGGGATAGAGGGAGGAGCAAGGTGCCGAGCGTGAGGATGAGGATGAGGCACGTAGCGAGGGTGGAGCTGAAGGGCGGGGTATGGGTGGAGGGGGCCACACTTGTTGTGGTGAGGCCGAGTTTGGGGGATGACGTGGAGGATGGGGATTTGGCGATGGAGGCGTTTGGGAATGATGGGATGTACGGGGAGGTTGTAGTGGAGCTGCTCAAGGCCAAGAGTTATTTACTGGAAATGAACTCTTTTTAG

Protein Analysis

324

Amino Acids

35.83

Weight (kDa)

5.38

Isoelectric Point (pI)

46.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 4 - 41 1.2e-07 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 576
AccB1I GGYRCC 1 cut(s) 748
AccBSI CCGCTC 2 cut(s) 285, 315
AccI GTMKAC 1 cut(s) 147
AccII CGCG 6 cut(s) 87, 153, 327, 539, 548, 689
AclWI GGATC 3 cut(s) 301, 427, 441
AcoI YGGCCR 2 cut(s) 258, 477
AcsI RAATTY 2 cut(s) 292, 404
AcuI CTGAAG 4 cut(s) 440, 530, 593, 815
AcyI GRCGYC 1 cut(s) 551
AfaI GTAC 1 cut(s) 908
AfiI CCNNNNNNNGG 4 cut(s) 256, 479, 574, 848
AflIII ACRYGT 1 cut(s) 687
AgsI TTSAA 1 cut(s) 106
AjiI CACGTC 2 cut(s) 426, 859
AjnI CCWGG 1 cut(s) 243
AleI CACNNNNGTG 1 cut(s) 644
AluBI AGCT 4 cut(s) 349, 401, 793, 928
AluI AGCT 4 cut(s) 349, 401, 793, 928
Alw21I GWGCWC 1 cut(s) 351
Alw26I GTCTC 2 cut(s) 61, 259
AlwI GGATC 3 cut(s) 301, 427, 441
AoxI GGCC 5 cut(s) 258, 477, 819, 839, 936
ApeKI GCWGC 2 cut(s) 81, 928
ApoI RAATTY 2 cut(s) 292, 404
AspA2I CCTAGG 1 cut(s) 436
AspLEI GCGC 3 cut(s) 89, 539, 541
AspS9I GGNCC 2 cut(s) 247, 819
AsuHPI GGTGA 4 cut(s) 526, 595, 658, 847
AvaII GGWCC 1 cut(s) 247
AvrII CCTAGG 1 cut(s) 436
BanI GGYRCC 1 cut(s) 748
BanII GRGCYC 1 cut(s) 351
BbsI GAAGAC 1 cut(s) 428
Bbv12I GWGCWC 1 cut(s) 351
BbvI GCAGC 2 cut(s) 68, 915
BccI CCATC 4 cut(s) 720, 860, 874, 893
BceAI ACGGC 1 cut(s) 74
BciT130I CCWGG 1 cut(s) 245
BcoDI GTCTC 2 cut(s) 61, 259
BfaI CTAG 2 cut(s) 251, 437
BfuAI ACCTGC 1 cut(s) 576
BglII AGATCT 1 cut(s) 42
BisI GCNGC 4 cut(s) 82, 85, 549, 929
BlnI CCTAGG 1 cut(s) 436
BlsI GCNGC 4 cut(s) 83, 86, 550, 930
Bme1390I CCNGG 1 cut(s) 245
Bme18I GGWCC 1 cut(s) 247
BmgBI CACGTC 2 cut(s) 426, 859
BmgT120I GGNCC 2 cut(s) 247, 819
BmiI GGNNCC 3 cut(s) 443, 750, 820
BmrFI CCNGG 1 cut(s) 245
BpiI GAAGAC 1 cut(s) 428
BpmI CTGGAG 2 cut(s) 23, 227
BpuEI CTTGAG 1 cut(s) 917
BsaAI YACGTR 1 cut(s) 778
BsaHI GRCGYC 1 cut(s) 551
BsaI GGTCTC 1 cut(s) 61
BsaJI CCNNGG 1 cut(s) 436
BsaWI WCCGGW 1 cut(s) 366
BsaXI ACNNNNNCTCC 4 cut(s) 41, 71, 674, 704
Bsc4I CCNNNNNNNGG 4 cut(s) 256, 479, 574, 848
Bse1I ACTGG 1 cut(s) 957
BseBI CCWGG 1 cut(s) 245
BseDI CCNNGG 1 cut(s) 436
BseGI GGATG 8 cut(s) 33, 135, 731, 768, 774, 859, 871, 909
BseLI CCNNNNNNNGG 4 cut(s) 256, 479, 574, 848
BseMII CTCAG 3 cut(s) 302, 608, 656
BseNI ACTGG 1 cut(s) 957
BsePI GCGCGC 1 cut(s) 537
BseRI GAGGAG 4 cut(s) 197, 719, 728, 754
BseXI GCAGC 2 cut(s) 68, 915
BsgI GTGCAG 1 cut(s) 694
Bsh1236I CGCG 6 cut(s) 87, 153, 327, 539, 548, 689
Bsh1285I CGRYCG 3 cut(s) 16, 151, 345
BshFI GGCC 5 cut(s) 260, 479, 821, 841, 938
BshNI GGYRCC 1 cut(s) 748
BsiEI CGRYCG 3 cut(s) 16, 151, 345
BsiHKAI GWGCWC 1 cut(s) 351
BsiSI CCGG 3 cut(s) 367, 480, 501
BslFI GGGAC 4 cut(s) 101, 394, 479, 635
BslI CCNNNNNNNGG 4 cut(s) 256, 479, 574, 848
BsmAI GTCTC 2 cut(s) 61, 259
BsmFI GGGAC 4 cut(s) 101, 394, 479, 635
BsnI GGCC 5 cut(s) 260, 479, 821, 841, 938
Bso31I GGTCTC 1 cut(s) 61
Bsp1286I GDGCHC 1 cut(s) 351
Bsp143I GATC 8 cut(s) 13, 42, 306, 342, 361, 432, 446, 543
BspANI GGCC 5 cut(s) 260, 479, 821, 841, 938
BspCNI CTCAG 3 cut(s) 301, 609, 657
BspFNI CGCG 6 cut(s) 87, 153, 327, 539, 548, 689
BspLI GGNNCC 3 cut(s) 443, 750, 820
BspMI ACCTGC 1 cut(s) 576
BspPI GGATC 3 cut(s) 301, 427, 441
BspT107I GGYRCC 1 cut(s) 748
BspTNI GGTCTC 1 cut(s) 61
BsrBI CCGCTC 2 cut(s) 285, 315
BsrI ACTGG 1 cut(s) 957
BssECI CCNNGG 1 cut(s) 436
BssHII GCGCGC 1 cut(s) 537
BssMI GATC 8 cut(s) 13, 42, 306, 342, 361, 432, 446, 543
BssNI GRCGYC 1 cut(s) 551
BssT1I CCWWGG 1 cut(s) 436
Bst2UI CCWGG 1 cut(s) 245
Bst4CI ACNGT 4 cut(s) 8, 145, 385, 643
Bst6I CTCTTC 1 cut(s) 714
BstACI GRCGYC 1 cut(s) 551
BstAPI GCANNNNNTGC 1 cut(s) 568
BstBAI YACGTR 1 cut(s) 778
BstC8I GCNNGC 3 cut(s) 399, 460, 539
BstDEI CTNAG 3 cut(s) 288, 617, 665
BstENI CCTNNNNNAGG 1 cut(s) 572
BstF5I GGATG 8 cut(s) 33, 135, 731, 768, 774, 859, 871, 909
BstFNI CGCG 6 cut(s) 87, 153, 327, 539, 548, 689
BstHHI GCGC 3 cut(s) 89, 539, 541
BstKTI GATC 8 cut(s) 16, 45, 309, 345, 364, 435, 449, 546
BstMAI GTCTC 2 cut(s) 61, 259
BstMBI GATC 8 cut(s) 13, 42, 306, 342, 361, 432, 446, 543
BstMCI CGRYCG 3 cut(s) 16, 151, 345
BstMWI GCNNNNNNNGC 9 cut(s) 333, 394, 497, 545, 554, 568, 598, 607, 884
BstNI CCWGG 1 cut(s) 245
BstSCI CCNGG 1 cut(s) 243
BstUI CGCG 6 cut(s) 87, 153, 327, 539, 548, 689
BstV1I GCAGC 2 cut(s) 68, 915
BstV2I GAAGAC 1 cut(s) 428
BstX2I RGATCY 2 cut(s) 42, 306
BstYI RGATCY 2 cut(s) 42, 306
BsuRI GGCC 5 cut(s) 260, 479, 821, 841, 938
BtgZI GCGATG 1 cut(s) 893
BtrI CACGTC 2 cut(s) 426, 859
BtsCI GGATG 8 cut(s) 33, 135, 731, 768, 774, 859, 871, 909
BveI ACCTGC 1 cut(s) 576
Cac8I GCNNGC 3 cut(s) 399, 460, 539
CfoI GCGC 3 cut(s) 89, 539, 541
Cfr13I GGNCC 2 cut(s) 247, 819
CseI GACGC 4 cut(s) 142, 316, 540, 695
Csp6I GTAC 1 cut(s) 907
CspCI CAANNNNNGTGG 2 cut(s) 811, 846
CviQI GTAC 1 cut(s) 907
DdeI CTNAG 3 cut(s) 288, 617, 665
DpnI GATC 8 cut(s) 15, 44, 308, 344, 363, 434, 448, 545
DpnII GATC 8 cut(s) 13, 42, 306, 342, 361, 432, 446, 543
EaeI YGGCCR 2 cut(s) 258, 477
Eam1104I CTCTTC 1 cut(s) 714
EarI CTCTTC 1 cut(s) 714
EciI GGCGGA 1 cut(s) 716
Ecl136II GAGCTC 1 cut(s) 349
Eco130I CCWWGG 1 cut(s) 436
Eco24I GRGCYC 1 cut(s) 351
Eco31I GGTCTC 1 cut(s) 61
Eco47I GGWCC 1 cut(s) 247
Eco53kI GAGCTC 1 cut(s) 349
Eco57I CTGAAG 4 cut(s) 440, 530, 593, 815
EcoICRI GAGCTC 1 cut(s) 349
EcoNI CCTNNNNNAGG 1 cut(s) 572
EcoO109I RGGNCCY 1 cut(s) 247
EcoRI GAATTC 2 cut(s) 292, 404
EcoRII CCWGG 1 cut(s) 243
EcoT14I CCWWGG 1 cut(s) 436
EcoT38I GRGCYC 1 cut(s) 351
ErhI CCWWGG 1 cut(s) 436
FaiI YATR 3 cut(s) 77, 158, 808
FaqI GGGAC 4 cut(s) 101, 394, 479, 635
FauI CCCGC 4 cut(s) 290, 307, 455, 794
FblI GTMKAC 1 cut(s) 147
Fnu4HI GCNGC 4 cut(s) 82, 85, 549, 929
FokI GGATG 8 cut(s) 20, 122, 738, 775, 781, 866, 878, 916
FriOI GRGCYC 1 cut(s) 351
Fsp4HI GCNGC 4 cut(s) 82, 85, 549, 929
FspBI CTAG 2 cut(s) 251, 437
GlaI GCGC 3 cut(s) 88, 538, 540
GluI GCNGC 4 cut(s) 82, 85, 549, 929
GsuI CTGGAG 2 cut(s) 23, 227
HaeIII GGCC 5 cut(s) 260, 479, 821, 841, 938
HapII CCGG 3 cut(s) 367, 480, 501
HgaI GACGC 4 cut(s) 142, 316, 540, 695
HhaI GCGC 3 cut(s) 89, 539, 541
Hin1I GRCGYC 1 cut(s) 551
Hin6I GCGC 3 cut(s) 87, 537, 539
HinP1I GCGC 3 cut(s) 87, 537, 539
HincII GTYRAC 1 cut(s) 148
HindII GTYRAC 1 cut(s) 148
HinfI GANTC 8 cut(s) 26, 50, 102, 128, 167, 173, 179, 605
HpaII CCGG 3 cut(s) 367, 480, 501
HphI GGTGA 4 cut(s) 526, 595, 658, 847
Hpy166II GTNNAC 2 cut(s) 148, 528
Hpy188III TCNNGA 4 cut(s) 40, 67, 340, 409
Hpy8I GTNNAC 2 cut(s) 148, 528
Hpy99I CGWCG 8 cut(s) 149, 194, 197, 215, 358, 455, 458, 556
HpyAV CCTTC 9 cut(s) 226, 328, 370, 505, 568, 623, 629, 649, 790
HpyCH4III ACNGT 4 cut(s) 8, 145, 385, 643
HpyCH4IV ACGT 4 cut(s) 195, 425, 777, 858
HpyCH4V TGCA 1 cut(s) 675
HpyF10VI GCNNNNNNNGC 9 cut(s) 333, 394, 497, 545, 554, 568, 598, 607, 884
HpyF3I CTNAG 3 cut(s) 288, 617, 665
HpySE526I ACGT 4 cut(s) 195, 425, 777, 858
Hsp92I GRCGYC 1 cut(s) 551
HspAI GCGC 3 cut(s) 87, 537, 539
Kzo9I GATC 8 cut(s) 13, 42, 306, 342, 361, 432, 446, 543
LmnI GCTCC 7 cut(s) 246, 320, 354, 589, 741, 790, 925
Lsp1109I GCAGC 2 cut(s) 68, 915
MaeI CTAG 2 cut(s) 251, 437
MaeII ACGT 4 cut(s) 195, 425, 777, 858
MalI GATC 8 cut(s) 15, 44, 308, 344, 363, 434, 448, 545
MbiI CCGCTC 2 cut(s) 285, 315
MboI GATC 8 cut(s) 13, 42, 306, 342, 361, 432, 446, 543
MboII GAAGA 3 cut(s) 37, 433, 731
MflI RGATCY 2 cut(s) 42, 306
MhlI GDGCHC 1 cut(s) 351
MluCI AATT 2 cut(s) 292, 404
MluI ACGCGT 1 cut(s) 687
MlyI GAGTC 5 cut(s) 20, 44, 122, 167, 173
MmeI TCCRAC 2 cut(s) 84, 201
MslI CAYNNNNRTG 1 cut(s) 644
MspA1I CMGCKG 1 cut(s) 462
MspI CCGG 3 cut(s) 367, 480, 501
MspR9I CCNGG 1 cut(s) 245
MvaI CCWGG 1 cut(s) 245
MvnI CGCG 6 cut(s) 87, 153, 327, 539, 548, 689
MwoI GCNNNNNNNGC 9 cut(s) 333, 394, 497, 545, 554, 568, 598, 607, 884
NdeII GATC 8 cut(s) 13, 42, 306, 342, 361, 432, 446, 543
NlaIV GGNNCC 3 cut(s) 443, 750, 820
NmeAIII GCCGAG 3 cut(s) 236, 777, 867
OliI CACNNNNGTG 1 cut(s) 644
PauI GCGCGC 1 cut(s) 537
PfeI GAWTC 3 cut(s) 102, 167, 605
PflFI GACNNNGTC 3 cut(s) 53, 152, 251
PfoI TCCNGGA 1 cut(s) 243
PkrI GCNGC 4 cut(s) 83, 86, 550, 930
Ple19I CGATCG 2 cut(s) 16, 345
PleI GAGTC 5 cut(s) 20, 44, 122, 167, 173
PpsI GAGTC 5 cut(s) 20, 44, 122, 167, 173
Ppu21I YACGTR 1 cut(s) 778
PpuMI RGGWCCY 1 cut(s) 247
Psp124BI GAGCTC 1 cut(s) 351
Psp5II RGGWCCY 1 cut(s) 247
Psp6I CCWGG 1 cut(s) 243
PspGI CCWGG 1 cut(s) 243
PspN4I GGNNCC 3 cut(s) 443, 750, 820
PspPI GGNCC 2 cut(s) 247, 819
PspPPI RGGWCCY 1 cut(s) 247
PsuI RGATCY 2 cut(s) 42, 306
PsyI GACNNNGTC 3 cut(s) 53, 152, 251
PteI GCGCGC 1 cut(s) 537
PvuI CGATCG 2 cut(s) 16, 345
RsaI GTAC 1 cut(s) 908
RsaNI GTAC 1 cut(s) 907
RseI CAYNNNNRTG 1 cut(s) 644
SacI GAGCTC 1 cut(s) 351
SalI GTCGAC 1 cut(s) 146
SatI GCNGC 4 cut(s) 82, 85, 549, 929
Sau3AI GATC 8 cut(s) 13, 42, 306, 342, 361, 432, 446, 543
Sau96I GGNCC 2 cut(s) 247, 819
SchI GAGTC 5 cut(s) 20, 44, 122, 167, 173
ScrFI CCNGG 1 cut(s) 245
SduI GDGCHC 1 cut(s) 351
SinI GGWCC 1 cut(s) 247
SmiMI CAYNNNNRTG 1 cut(s) 644
SmlI CTYRAG 1 cut(s) 932
SmoI CTYRAG 1 cut(s) 932
Sse9I AATT 2 cut(s) 292, 404
SspMI CTAG 2 cut(s) 251, 437
SstI GAGCTC 1 cut(s) 351
StyD4I CCNGG 1 cut(s) 243
StyI CCWWGG 1 cut(s) 436
TaaI ACNGT 4 cut(s) 8, 145, 385, 643
TaiI ACGT 4 cut(s) 198, 428, 780, 861
TaqI TCGA 5 cut(s) 12, 48, 147, 341, 345
TaqII GACCGA 1 cut(s) 43
TasI AATT 2 cut(s) 292, 404
TauI GCSGC 2 cut(s) 87, 551
TfiI GAWTC 3 cut(s) 102, 167, 605
TseI GCWGC 2 cut(s) 81, 928
TspGWI ACGGA 1 cut(s) 439
Tth111I GACNNNGTC 3 cut(s) 53, 152, 251
VpaK11BI GGWCC 1 cut(s) 247
XagI CCTNNNNNAGG 1 cut(s) 572
XapI RAATTY 2 cut(s) 292, 404
XcmI CCANNNNNNNNNTGG 1 cut(s) 829
XmaJI CCTAGG 1 cut(s) 436
XmiI GTMKAC 1 cut(s) 147
XspI CTAG 2 cut(s) 251, 437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.