Rh7DG467900

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0009029
Physical Location & Seq
Reverse (-)
66758266 .. 66759255
990 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG467900.1

Sequence Viewer

Length: 990 bp
ATGGACGGCTTCGATCGGCTCCCGGACCCGGTGATCCTCCAGATCTTCGACCTGGTCTCGGACACCAAGACCCTCATTCGCTGCCTCGCCGTTTCGAAACGCTTCAACTCCCTCGTCCCCCACACCGACTCCCTAGTCCTCACCGTTGACCGCGTCATCTCCTCCGACTCCGACTCCGACTCAGACGACGACGTTTCCAACCTCCTCGCCTCCGCCTTCCGCTACATCCTCAAGTCTCTCCACAGCCTCGTCTCCCCCAAGCCCAACAACAACAACGACAACGACAACCCGACCCGGACCCGGATCCCCAACTGCCCCGCCCAGATCCTCCGCTGCTTCCACCGCGTCAAGCGCCTTGCGATCGACCTCCCCTCCGGCGACCTCCGCCTCGAGAAGGGGACCGTTCTCAAGTGGAGAGCCGAGTTCGGGAAAACCCTCCGGACCTGCGTCATCCTAGGGTTCCGATCCGTCGGGGAGACTCAGTCGCCGGCGACGGCCGGAGCTGAGCCCGACTTCGCCGGCGGGCTGAAGGTGCGGGTGGTGTGGACGATCAGCGCTCTGATCGCGGCGTCGGCGAGGCACTATTTGCTGAAGGAGGTGGTGAGGGAGCAGAGCGGGCTCGAGTGTCTGGTTCTGAGGGACAGAGAGGCGGAGGGGACGGTGGTGATGGAGAAGGAGGGGTTGAGGGAGTGTAGAGAAGAAGGTGAGGAGTTTGCTGATGAGGAGGAGGGGTTGGATAGGTGCAGGACTAGAGTGCCTAGTGTGAGGATGAGGATGAGGCATGTGCCGAGATTGGAGCTCAAAGGCGGGCTTTGGGTCGAGGGTGCGACGCTCGTGGTGGTCCGGCCCAGTTTGGAAGGCGGCGGTGGCGACATGGCGGTGGAGGATGGGGAATTGGCGTTGAGGGCATTTGGGAATGGTTTGTATGGGGAGGTTGCGCAGAAGCTGCTCAAGGCCAGAAGCTATTTGCTGGAAATGAACTCATTCTGA

Protein Analysis

329

Amino Acids

36.51

Weight (kDa)

5.88

Isoelectric Point (pI)

41.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 4 - 41 1.1e-07 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 134
Acc16I TGCGCA 1 cut(s) 939
Acc36I ACCTGC 1 cut(s) 452
AccBSI CCGCTC 1 cut(s) 615
AccII CGCG 3 cut(s) 153, 345, 566
AccIII TCCGGA 1 cut(s) 438
AclWI GGATC 5 cut(s) 28, 298, 311, 319, 459
AcoI YGGCCR 1 cut(s) 495
AcuI CTGAAG 2 cut(s) 548, 611
AcyI GRCGYC 1 cut(s) 569
AfeI AGCGCT 1 cut(s) 556
AfiI CCNNNNNNNGG 5 cut(s) 28, 58, 300, 394, 426
AgsI TTSAA 1 cut(s) 106
AjnI CCWGG 1 cut(s) 51
AluBI AGCT 4 cut(s) 503, 799, 946, 963
AluI AGCT 4 cut(s) 503, 799, 946, 963
Alw21I GWGCWC 1 cut(s) 801
Alw26I GTCTC 4 cut(s) 61, 240, 256, 470
AlwI GGATC 5 cut(s) 28, 298, 311, 319, 459
AlwNI CAGNNNCTG 1 cut(s) 946
Ama87I CYCGRG 2 cut(s) 389, 620
Aor13HI TCCGGA 1 cut(s) 438
Aor51HI AGCGCT 1 cut(s) 556
AoxI GGCC 3 cut(s) 495, 845, 954
ApeKI GCWGC 3 cut(s) 81, 333, 946
Asp700I GAANNNNTTC 2 cut(s) 101, 983
AspA2I CCTAGG 1 cut(s) 454
AspLEI GCGC 3 cut(s) 354, 557, 940
AspS9I GGNCC 6 cut(s) 25, 297, 399, 441, 841, 846
AsuC2I CCSGG 4 cut(s) 23, 29, 295, 301
AsuHPI GGTGA 5 cut(s) 43, 133, 613, 676, 716
AsuII TTCGAA 1 cut(s) 95
AvaI CYCGRG 2 cut(s) 389, 620
AvaII GGWCC 5 cut(s) 25, 297, 399, 441, 841
AvrII CCTAGG 1 cut(s) 454
BamHI GGATCC 1 cut(s) 303
BanII GRGCYC 3 cut(s) 510, 621, 801
BauI CACGAG 1 cut(s) 833
Bbv12I GWGCWC 1 cut(s) 801
BbvI GCAGC 3 cut(s) 68, 320, 933
BccI CCATC 2 cut(s) 661, 881
BceAI ACGGC 3 cut(s) 22, 74, 510
BciT130I CCWGG 1 cut(s) 53
BcnI CCSGG 4 cut(s) 23, 29, 295, 301
BcoDI GTCTC 4 cut(s) 61, 240, 256, 470
BfaI CTAG 4 cut(s) 134, 455, 750, 759
BfoI RGCGCY 2 cut(s) 355, 558
BfuAI ACCTGC 1 cut(s) 452
BglII AGATCT 1 cut(s) 42
BisI GCNGC 5 cut(s) 82, 334, 567, 862, 947
BlnI CCTAGG 1 cut(s) 454
BlpI GCTNAGC 1 cut(s) 504
BlsI GCNGC 5 cut(s) 83, 335, 568, 863, 948
Bme1390I CCNGG 5 cut(s) 23, 29, 53, 295, 301
Bme18I GGWCC 5 cut(s) 25, 297, 399, 441, 841
BmeT110I CYCGRG 2 cut(s) 389, 620
BmgT120I GGNCC 6 cut(s) 25, 297, 399, 441, 841, 846
BmiI GGNNCC 6 cut(s) 20, 27, 299, 305, 400, 461
BmrFI CCNGG 5 cut(s) 23, 29, 53, 295, 301
BmrI ACTGGG 1 cut(s) 843
BmuI ACTGGG 1 cut(s) 843
BoxI GACNNNNGTC 1 cut(s) 446
BpmI CTGGAG 1 cut(s) 23
Bpu1102I GCTNAGC 1 cut(s) 504
Bpu14I TTCGAA 1 cut(s) 95
BpuEI CTTGAG 3 cut(s) 215, 392, 935
BpuMI CCSGG 4 cut(s) 23, 29, 295, 301
BsaHI GRCGYC 1 cut(s) 569
BsaI GGTCTC 1 cut(s) 61
BsaJI CCNNGG 1 cut(s) 454
BsaWI WCCGGW 1 cut(s) 438
Bsc4I CCNNNNNNNGG 5 cut(s) 28, 58, 300, 394, 426
Bse118I RCCGGY 2 cut(s) 487, 518
Bse1I ACTGG 1 cut(s) 849
BseAI TCCGGA 1 cut(s) 438
BseBI CCWGG 1 cut(s) 53
BseDI CCNNGG 1 cut(s) 454
BseGI GGATG 5 cut(s) 225, 450, 774, 780, 892
BseLI CCNNNNNNNGG 5 cut(s) 28, 58, 300, 394, 426
BseMII CTCAG 4 cut(s) 195, 494, 495, 626
BseNI ACTGG 1 cut(s) 849
BseRI GAGGAG 5 cut(s) 151, 194, 722, 737, 740
BseX3I CGGCCG 1 cut(s) 495
BseXI GCAGC 3 cut(s) 68, 320, 933
BsgI GTGCAG 1 cut(s) 763
Bsh1236I CGCG 3 cut(s) 153, 345, 566
Bsh1285I CGRYCG 3 cut(s) 16, 363, 498
BshFI GGCC 3 cut(s) 497, 847, 956
BsiEI CGRYCG 3 cut(s) 16, 363, 498
BsiHKAI GWGCWC 1 cut(s) 801
BsiHKCI CYCGRG 2 cut(s) 389, 620
BslFI GGGAC 4 cut(s) 101, 412, 653, 670
BslI CCNNNNNNNGG 5 cut(s) 28, 58, 300, 394, 426
BsmAI GTCTC 4 cut(s) 61, 240, 256, 470
BsmBI CGTCTC 1 cut(s) 256
BsmFI GGGAC 4 cut(s) 101, 412, 653, 670
BsnI GGCC 3 cut(s) 497, 847, 956
Bso31I GGTCTC 1 cut(s) 61
BsoBI CYCGRG 2 cut(s) 389, 620
Bsp119I TTCGAA 1 cut(s) 95
Bsp1286I GDGCHC 3 cut(s) 510, 621, 801
Bsp13I TCCGGA 1 cut(s) 438
Bsp143I GATC 9 cut(s) 13, 33, 42, 303, 324, 360, 464, 549, 561
Bsp1720I GCTNAGC 1 cut(s) 504
BspANI GGCC 3 cut(s) 497, 847, 956
BspCNI CTCAG 4 cut(s) 194, 493, 496, 627
BspEI TCCGGA 1 cut(s) 438
BspFNI CGCG 3 cut(s) 153, 345, 566
BspLI GGNNCC 6 cut(s) 20, 27, 299, 305, 400, 461
BspMI ACCTGC 1 cut(s) 452
BspPI GGATC 5 cut(s) 28, 298, 311, 319, 459
BspT104I TTCGAA 1 cut(s) 95
BspTNI GGTCTC 1 cut(s) 61
BsrBI CCGCTC 1 cut(s) 615
BsrFI RCCGGY 2 cut(s) 487, 518
BsrI ACTGG 1 cut(s) 849
BssAI RCCGGY 2 cut(s) 487, 518
BssECI CCNNGG 1 cut(s) 454
BssMI GATC 9 cut(s) 13, 33, 42, 303, 324, 360, 464, 549, 561
BssNI GRCGYC 1 cut(s) 569
BssSI CACGAG 1 cut(s) 833
BssT1I CCWWGG 1 cut(s) 454
Bst2BI CACGAG 1 cut(s) 833
Bst2UI CCWGG 1 cut(s) 53
Bst4CI ACNGT 3 cut(s) 145, 403, 661
BstACI GRCGYC 1 cut(s) 569
BstAPI GCANNNNNTGC 2 cut(s) 586, 946
BstBI TTCGAA 1 cut(s) 95
BstC8I GCNNGC 5 cut(s) 489, 520, 524, 617, 809
BstDEI CTNAG 4 cut(s) 181, 480, 504, 635
BstENI CCTNNNNNAGG 1 cut(s) 392
BstF5I GGATG 5 cut(s) 225, 450, 774, 780, 892
BstFNI CGCG 3 cut(s) 153, 345, 566
BstH2I RGCGCY 2 cut(s) 355, 558
BstHHI GCGC 3 cut(s) 354, 557, 940
BstKTI GATC 9 cut(s) 16, 36, 45, 306, 327, 363, 467, 552, 564
BstMAI GTCTC 4 cut(s) 61, 240, 256, 470
BstMBI GATC 9 cut(s) 13, 33, 42, 303, 324, 360, 464, 549, 561
BstMCI CGRYCG 3 cut(s) 16, 363, 498
BstNI CCWGG 1 cut(s) 53
BstNSI RCATGY 1 cut(s) 785
BstPAI GACNNNNGTC 1 cut(s) 446
BstSCI CCNGG 5 cut(s) 21, 27, 51, 293, 299
BstUI CGCG 3 cut(s) 153, 345, 566
BstV1I GCAGC 3 cut(s) 68, 320, 933
BstX2I RGATCY 3 cut(s) 42, 303, 324
BstYI RGATCY 3 cut(s) 42, 303, 324
BstZI CGGCCG 1 cut(s) 495
BsuRI GGCC 3 cut(s) 497, 847, 956
BtsCI GGATG 5 cut(s) 225, 450, 774, 780, 892
BveI ACCTGC 1 cut(s) 452
Cac8I GCNNGC 5 cut(s) 489, 520, 524, 617, 809
CaiI CAGNNNCTG 1 cut(s) 946
CfoI GCGC 3 cut(s) 354, 557, 940
Cfr10I RCCGGY 2 cut(s) 487, 518
Cfr13I GGNCC 6 cut(s) 25, 297, 399, 441, 841, 846
CseI GACGC 5 cut(s) 142, 334, 436, 558, 838
CsiI ACCWGGT 1 cut(s) 51
CviAII CATG 2 cut(s) 782, 874
DdeI CTNAG 4 cut(s) 181, 480, 504, 635
DpnI GATC 9 cut(s) 15, 35, 44, 305, 326, 362, 466, 551, 563
DpnII GATC 9 cut(s) 13, 33, 42, 303, 324, 360, 464, 549, 561
DrdI GACNNNNNNGTC 1 cut(s) 134
DseDI GACNNNNNNGTC 1 cut(s) 134
EaeI YGGCCR 1 cut(s) 495
EagI CGGCCG 1 cut(s) 495
EciI GGCGGA 3 cut(s) 202, 374, 665
Ecl136II GAGCTC 1 cut(s) 799
EclXI CGGCCG 1 cut(s) 495
Eco130I CCWWGG 1 cut(s) 454
Eco24I GRGCYC 3 cut(s) 510, 621, 801
Eco31I GGTCTC 1 cut(s) 61
Eco47I GGWCC 5 cut(s) 25, 297, 399, 441, 841
Eco47III AGCGCT 1 cut(s) 556
Eco52I CGGCCG 1 cut(s) 495
Eco53kI GAGCTC 1 cut(s) 799
Eco57I CTGAAG 2 cut(s) 548, 611
Eco88I CYCGRG 2 cut(s) 389, 620
EcoICRI GAGCTC 1 cut(s) 799
EcoNI CCTNNNNNAGG 1 cut(s) 392
EcoRII CCWGG 1 cut(s) 51
EcoT14I CCWWGG 1 cut(s) 454
EcoT38I GRGCYC 3 cut(s) 510, 621, 801
ErhI CCWWGG 1 cut(s) 454
Esp3I CGTCTC 1 cut(s) 256
FaeI CATG 2 cut(s) 785, 877
FaiI YATR 3 cut(s) 783, 875, 927
FalI AAGNNNNNCTT 2 cut(s) 795, 827
FaqI GGGAC 4 cut(s) 101, 412, 653, 670
FatI CATG 2 cut(s) 781, 873
FauI CCCGC 5 cut(s) 325, 515, 528, 608, 800
Fnu4HI GCNGC 5 cut(s) 82, 334, 567, 862, 947
FokI GGATG 5 cut(s) 212, 437, 781, 787, 899
FriOI GRGCYC 3 cut(s) 510, 621, 801
Fsp4HI GCNGC 5 cut(s) 82, 334, 567, 862, 947
FspBI CTAG 4 cut(s) 134, 455, 750, 759
FspI TGCGCA 1 cut(s) 939
GlaI GCGC 3 cut(s) 353, 556, 939
GluI GCNGC 5 cut(s) 82, 334, 567, 862, 947
GsuI CTGGAG 1 cut(s) 23
HaeII RGCGCY 2 cut(s) 355, 558
HaeIII GGCC 3 cut(s) 497, 847, 956
HgaI GACGC 5 cut(s) 142, 334, 436, 558, 838
HhaI GCGC 3 cut(s) 354, 557, 940
Hin1I GRCGYC 1 cut(s) 569
Hin1II CATG 2 cut(s) 785, 877
Hin6I GCGC 3 cut(s) 352, 555, 938
HinP1I GCGC 3 cut(s) 352, 555, 938
HincII GTYRAC 1 cut(s) 148
HindII GTYRAC 1 cut(s) 148
HinfI GANTC 5 cut(s) 128, 167, 173, 179, 478
HphI GGTGA 5 cut(s) 43, 133, 613, 676, 716
Hpy166II GTNNAC 2 cut(s) 148, 546
Hpy188I TCNGA 9 cut(s) 61, 166, 172, 178, 184, 464, 561, 636, 989
Hpy188III TCNNGA 4 cut(s) 40, 391, 427, 439
Hpy8I GTNNAC 2 cut(s) 148, 546
Hpy99I CGWCG 6 cut(s) 191, 194, 473, 496, 574, 832
HpyAV CCTTC 7 cut(s) 226, 388, 523, 586, 667, 695, 851
HpyCH4III ACNGT 3 cut(s) 145, 403, 661
HpyCH4IV ACGT 1 cut(s) 192
HpyCH4V TGCA 1 cut(s) 744
HpyF3I CTNAG 4 cut(s) 181, 480, 504, 635
HpySE526I ACGT 1 cut(s) 192
Hsp92I GRCGYC 1 cut(s) 569
Hsp92II CATG 2 cut(s) 785, 877
HspAI GCGC 3 cut(s) 352, 555, 938
Kpn2I TCCGGA 1 cut(s) 438
KroI GCCGGC 2 cut(s) 487, 518
KroNI GCCGGC 2 cut(s) 489, 520
Kzo9I GATC 9 cut(s) 13, 33, 42, 303, 324, 360, 464, 549, 561
LmnI GCTCC 4 cut(s) 24, 500, 607, 796
Lsp1109I GCAGC 3 cut(s) 68, 320, 933
MabI ACCWGGT 1 cut(s) 51
MaeI CTAG 4 cut(s) 134, 455, 750, 759
MaeII ACGT 1 cut(s) 192
MalI GATC 9 cut(s) 15, 35, 44, 305, 326, 362, 466, 551, 563
MbiI CCGCTC 1 cut(s) 615
MboI GATC 9 cut(s) 13, 33, 42, 303, 324, 360, 464, 549, 561
MboII GAAGA 2 cut(s) 37, 710
MflI RGATCY 3 cut(s) 42, 303, 324
MhlI GDGCHC 3 cut(s) 510, 621, 801
MluCI AATT 1 cut(s) 893
MlyI GAGTC 5 cut(s) 122, 161, 167, 173, 472
MmeI TCCRAC 5 cut(s) 189, 195, 201, 222, 714
MreI CGCCGGCG 2 cut(s) 487, 518
MroI TCCGGA 1 cut(s) 438
MroNI GCCGGC 2 cut(s) 487, 518
MroXI GAANNNNTTC 2 cut(s) 101, 983
MslI CAYNNNNRTG 1 cut(s) 878
MspA1I CMGCKG 1 cut(s) 333
MspR9I CCNGG 5 cut(s) 23, 29, 53, 295, 301
MvaI CCWGG 1 cut(s) 53
MvnI CGCG 3 cut(s) 153, 345, 566
NaeI GCCGGC 2 cut(s) 489, 520
NciI CCSGG 4 cut(s) 23, 29, 295, 301
NdeII GATC 9 cut(s) 13, 33, 42, 303, 324, 360, 464, 549, 561
NgoMIV GCCGGC 2 cut(s) 487, 518
NlaIII CATG 2 cut(s) 785, 877
NlaIV GGNNCC 6 cut(s) 20, 27, 299, 305, 400, 461
NmeAIII GCCGAG 2 cut(s) 445, 813
NsbI TGCGCA 1 cut(s) 939
NspI RCATGY 1 cut(s) 785
NspV TTCGAA 1 cut(s) 95
PaeR7I CTCGAG 2 cut(s) 389, 620
PdiI GCCGGC 2 cut(s) 489, 520
PdmI GAANNNNTTC 2 cut(s) 101, 983
PflFI GACNNNGTC 3 cut(s) 53, 152, 481
PfoI TCCNGGA 1 cut(s) 21
PkrI GCNGC 5 cut(s) 83, 335, 568, 863, 948
Ple19I CGATCG 2 cut(s) 16, 363
PleI GAGTC 5 cut(s) 122, 161, 167, 173, 472
PpsI GAGTC 5 cut(s) 122, 161, 167, 173, 472
PshAI GACNNNNGTC 1 cut(s) 446
Psp124BI GAGCTC 1 cut(s) 801
Psp6I CCWGG 1 cut(s) 51
PspGI CCWGG 1 cut(s) 51
PspN4I GGNNCC 6 cut(s) 20, 27, 299, 305, 400, 461
PspPI GGNCC 6 cut(s) 25, 297, 399, 441, 841, 846
PspXI VCTCGAGB 1 cut(s) 620
PstNI CAGNNNCTG 1 cut(s) 946
PsuI RGATCY 3 cut(s) 42, 303, 324
PsyI GACNNNGTC 3 cut(s) 53, 152, 481
PvuI CGATCG 2 cut(s) 16, 363
RseI CAYNNNNRTG 1 cut(s) 878
SacI GAGCTC 1 cut(s) 801
SatI GCNGC 5 cut(s) 82, 334, 567, 862, 947
Sau3AI GATC 9 cut(s) 13, 33, 42, 303, 324, 360, 464, 549, 561
Sau96I GGNCC 6 cut(s) 25, 297, 399, 441, 841, 846
SchI GAGTC 5 cut(s) 122, 161, 167, 173, 472
ScrFI CCNGG 5 cut(s) 23, 29, 53, 295, 301
SduI GDGCHC 3 cut(s) 510, 621, 801
SexAI ACCWGGT 1 cut(s) 51
Sfr274I CTCGAG 2 cut(s) 389, 620
SfuI TTCGAA 1 cut(s) 95
SgrAI CRCCGGYG 2 cut(s) 487, 518
SinI GGWCC 5 cut(s) 25, 297, 399, 441, 841
SlaI CTCGAG 2 cut(s) 389, 620
SmiMI CAYNNNNRTG 1 cut(s) 878
SmlI CTYRAG 5 cut(s) 230, 389, 407, 620, 950
SmoI CTYRAG 5 cut(s) 230, 389, 407, 620, 950
Sse9I AATT 1 cut(s) 893
SspMI CTAG 4 cut(s) 134, 455, 750, 759
SstI GAGCTC 1 cut(s) 801
StyD4I CCNGG 5 cut(s) 21, 27, 51, 293, 299
StyI CCWWGG 1 cut(s) 454
TaaI ACNGT 3 cut(s) 145, 403, 661
TaiI ACGT 1 cut(s) 195
TaqI TCGA 7 cut(s) 12, 48, 95, 363, 390, 621, 819
TasI AATT 1 cut(s) 893
TauI GCSGC 2 cut(s) 569, 864
TseI GCWGC 3 cut(s) 81, 333, 946
TspGWI ACGGA 1 cut(s) 457
Tth111I GACNNNGTC 3 cut(s) 53, 152, 481
VpaK11BI GGWCC 5 cut(s) 25, 297, 399, 441, 841
XagI CCTNNNNNAGG 1 cut(s) 392
XceI RCATGY 1 cut(s) 785
XhoI CTCGAG 2 cut(s) 389, 620
XmaJI CCTAGG 1 cut(s) 454
XmnI GAANNNNTTC 2 cut(s) 101, 983
XspI CTAG 4 cut(s) 134, 455, 750, 759
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.