RchiOBHm_Chr7g0239491

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0009029
Physical Location & Seq
Reverse (-)
64996238 .. 64997632
1395 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ21463

Sequence Viewer

Length: 987 bp
ATGGAAATGGACGGCTTCGATCGGCTCCCGGACCCGGTGATCCTCCAGATCTTCGACCTGGTCTCGGACACCAAGACCCTCATTCGCTGCCTCGCCGTTTCGAAACGCTTCAACTCCCTCGTCCCCCACACCGACTCCCTAGTCCTCACCGTCGACCGCGTCATCTCCTCCGACTCCGACTCCGACTCAGACGACGACGTTTCCAACCTCCTCGCCTCCGCCTTCCGCTACATCCTCAAGTCTCTCCACAGCCTCGTCTGCCCCAAGCCCAACAACAACAACAACCCGACCCGGACCCGGATCCCCAACTGCCCCGCCCAGATCCTCCGCTGCTTCCACCGCGTCAAGCGCCTCGCGATCGACCTCCCCTCCGGCGACCTCCGCCTCGAGAAGGGGACCGTTCTCAAGTGGAGAGCCGAGTTCGGGAAAACCCTCCGGACCTGCGTCATCCTAGGGTTCCGATCCGTCGGGGAGACTCCGTCGCAGGTGACGGCCGGAGCTGAGCCCGACTTAACCGGCGGGCTCAAGGTGCGGGTGGTGTGGACGATCAGTGCTCTGATCGCGGCGTCGGCGAGGCACTATTTGTTGAAAGAGGTGGTGAGGGAGCAGAGCGGGCTCGAATGTCTGGTTCTGAGGGACAGAGAGGCGGAGGGGACGGTGGTGATGGAGAAGGAGGGGTTGAGGGAGTGTAGAGAAGAAGGTGAGGAGTTTGCTGATGAGGACGAGGGGTTGGATAGGTGCAGGACTAGAGTGCCTAGTGTGAGGATGAGGATGAGGCATGTGCCCAGATTGGAGCTCAAAGGCGGGCTTTGGGTCGAGGGTGCGACGCTCGTGGTGGTCCGGCCCAGTTTGGTAGGCGGCGGTGGCGACATGGCGGTGGAGGATGGGGAATTGGCGTTGAGGGCATTTGGGAATGGTTTGTATGGGGAGGTTGCGCAGAAGCTGCTCAAGGCCAGAAGCTATTTGCTGGAAATGAACTCATTCTGA

Protein Analysis

328

Amino Acids

36.42

Weight (kDa)

6.28

Isoelectric Point (pI)

36.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 6 - 43 1.1e-07 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 475
AasI GACNNNNNNGTC 1 cut(s) 140
Acc16I TGCGCA 1 cut(s) 936
Acc36I ACCTGC 2 cut(s) 449, 475
AccBSI CCGCTC 1 cut(s) 612
AccI GTMKAC 1 cut(s) 153
AccII CGCG 4 cut(s) 159, 342, 356, 563
AccIII TCCGGA 1 cut(s) 435
AclWI GGATC 5 cut(s) 34, 295, 308, 316, 456
AcoI YGGCCR 1 cut(s) 492
AcyI GRCGYC 1 cut(s) 566
AfiI CCNNNNNNNGG 5 cut(s) 34, 64, 297, 391, 423
AgsI TTSAA 2 cut(s) 112, 589
AjnI CCWGG 1 cut(s) 57
AluBI AGCT 4 cut(s) 500, 796, 943, 960
AluI AGCT 4 cut(s) 500, 796, 943, 960
Alw21I GWGCWC 2 cut(s) 556, 798
Alw26I GTCTC 3 cut(s) 67, 246, 467
AlwI GGATC 5 cut(s) 34, 295, 308, 316, 456
AlwNI CAGNNNCTG 1 cut(s) 943
Ama87I CYCGRG 1 cut(s) 386
Aor13HI TCCGGA 1 cut(s) 435
AoxI GGCC 3 cut(s) 492, 842, 951
ApeKI GCWGC 3 cut(s) 87, 330, 943
Asp700I GAANNNNTTC 2 cut(s) 107, 980
AspA2I CCTAGG 1 cut(s) 451
AspLEI GCGC 2 cut(s) 351, 937
AspS9I GGNCC 6 cut(s) 31, 294, 396, 438, 838, 843
AsuC2I CCSGG 4 cut(s) 29, 35, 292, 298
AsuHPI GGTGA 6 cut(s) 49, 139, 499, 610, 673, 713
AsuII TTCGAA 1 cut(s) 101
AvaI CYCGRG 1 cut(s) 386
AvaII GGWCC 5 cut(s) 31, 294, 396, 438, 838
AvrII CCTAGG 1 cut(s) 451
BaeGI GKGCMC 1 cut(s) 786
BamHI GGATCC 1 cut(s) 300
BanII GRGCYC 4 cut(s) 507, 525, 618, 798
BauI CACGAG 1 cut(s) 830
Bbv12I GWGCWC 2 cut(s) 556, 798
BbvI GCAGC 3 cut(s) 74, 317, 930
BccI CCATC 2 cut(s) 658, 878
BceAI ACGGC 3 cut(s) 28, 80, 507
BciT130I CCWGG 1 cut(s) 59
BcnI CCSGG 4 cut(s) 29, 35, 292, 298
BcoDI GTCTC 3 cut(s) 67, 246, 467
BfaI CTAG 4 cut(s) 140, 452, 747, 756
BfoI RGCGCY 1 cut(s) 352
BfuAI ACCTGC 2 cut(s) 449, 475
BglII AGATCT 1 cut(s) 48
BisI GCNGC 5 cut(s) 88, 331, 564, 859, 944
BlnI CCTAGG 1 cut(s) 451
BlpI GCTNAGC 1 cut(s) 501
BlsI GCNGC 5 cut(s) 89, 332, 565, 860, 945
Bme1390I CCNGG 5 cut(s) 29, 35, 59, 292, 298
Bme18I GGWCC 5 cut(s) 31, 294, 396, 438, 838
BmeT110I CYCGRG 1 cut(s) 386
BmgT120I GGNCC 6 cut(s) 31, 294, 396, 438, 838, 843
BmiI GGNNCC 6 cut(s) 26, 33, 296, 302, 397, 458
BmrFI CCNGG 5 cut(s) 29, 35, 59, 292, 298
BmrI ACTGGG 1 cut(s) 840
BmuI ACTGGG 1 cut(s) 840
BoxI GACNNNNGTC 1 cut(s) 443
BpmI CTGGAG 1 cut(s) 29
Bpu1102I GCTNAGC 1 cut(s) 501
Bpu14I TTCGAA 1 cut(s) 101
BpuEI CTTGAG 4 cut(s) 221, 389, 509, 932
BpuMI CCSGG 4 cut(s) 29, 35, 292, 298
BsaHI GRCGYC 1 cut(s) 566
BsaI GGTCTC 1 cut(s) 67
BsaJI CCNNGG 1 cut(s) 451
BsaWI WCCGGW 1 cut(s) 435
BsaXI ACNNNNNCTCC 8 cut(s) 119, 149, 164, 194, 353, 383, 464, 494
Bsc4I CCNNNNNNNGG 5 cut(s) 34, 64, 297, 391, 423
Bse118I RCCGGY 1 cut(s) 515
Bse1I ACTGG 1 cut(s) 846
BseAI TCCGGA 1 cut(s) 435
BseBI CCWGG 1 cut(s) 59
BseDI CCNNGG 1 cut(s) 451
BseGI GGATG 5 cut(s) 231, 447, 771, 777, 889
BseLI CCNNNNNNNGG 5 cut(s) 34, 64, 297, 391, 423
BseMII CTCAG 3 cut(s) 201, 492, 623
BseNI ACTGG 1 cut(s) 846
BseRI GAGGAG 3 cut(s) 157, 200, 719
BseSI GKGCMC 1 cut(s) 786
BseX3I CGGCCG 1 cut(s) 492
BseXI GCAGC 3 cut(s) 74, 317, 930
BsgI GTGCAG 1 cut(s) 760
Bsh1236I CGCG 4 cut(s) 159, 342, 356, 563
Bsh1285I CGRYCG 4 cut(s) 22, 157, 360, 495
BshFI GGCC 3 cut(s) 494, 844, 953
BsiEI CGRYCG 4 cut(s) 22, 157, 360, 495
BsiHKAI GWGCWC 2 cut(s) 556, 798
BsiHKCI CYCGRG 1 cut(s) 386
BsiSI CCGG 9 cut(s) 29, 35, 292, 298, 372, 436, 495, 516, 841
BslFI GGGAC 4 cut(s) 107, 409, 650, 667
BslI CCNNNNNNNGG 5 cut(s) 34, 64, 297, 391, 423
BsmAI GTCTC 3 cut(s) 67, 246, 467
BsmFI GGGAC 4 cut(s) 107, 409, 650, 667
BsnI GGCC 3 cut(s) 494, 844, 953
Bso31I GGTCTC 1 cut(s) 67
BsoBI CYCGRG 1 cut(s) 386
Bsp119I TTCGAA 1 cut(s) 101
Bsp1286I GDGCHC 6 cut(s) 507, 525, 556, 618, 786, 798
Bsp13I TCCGGA 1 cut(s) 435
Bsp143I GATC 9 cut(s) 19, 39, 48, 300, 321, 357, 461, 546, 558
Bsp1720I GCTNAGC 1 cut(s) 501
Bsp68I TCGCGA 1 cut(s) 356
BspANI GGCC 3 cut(s) 494, 844, 953
BspCNI CTCAG 3 cut(s) 200, 493, 624
BspEI TCCGGA 1 cut(s) 435
BspFNI CGCG 4 cut(s) 159, 342, 356, 563
BspLI GGNNCC 6 cut(s) 26, 33, 296, 302, 397, 458
BspMI ACCTGC 2 cut(s) 449, 475
BspPI GGATC 5 cut(s) 34, 295, 308, 316, 456
BspT104I TTCGAA 1 cut(s) 101
BspTNI GGTCTC 1 cut(s) 67
BsrBI CCGCTC 1 cut(s) 612
BsrFI RCCGGY 1 cut(s) 515
BsrI ACTGG 1 cut(s) 846
BssAI RCCGGY 1 cut(s) 515
BssECI CCNNGG 1 cut(s) 451
BssMI GATC 9 cut(s) 19, 39, 48, 300, 321, 357, 461, 546, 558
BssNI GRCGYC 1 cut(s) 566
BssSI CACGAG 1 cut(s) 830
BssT1I CCWWGG 1 cut(s) 451
Bst2BI CACGAG 1 cut(s) 830
Bst2UI CCWGG 1 cut(s) 59
Bst4CI ACNGT 3 cut(s) 151, 400, 658
BstACI GRCGYC 1 cut(s) 566
BstAPI GCANNNNNTGC 1 cut(s) 943
BstBI TTCGAA 1 cut(s) 101
BstC8I GCNNGC 3 cut(s) 521, 614, 806
BstDEI CTNAG 3 cut(s) 187, 501, 632
BstENI CCTNNNNNAGG 1 cut(s) 389
BstF5I GGATG 5 cut(s) 231, 447, 771, 777, 889
BstFNI CGCG 4 cut(s) 159, 342, 356, 563
BstH2I RGCGCY 1 cut(s) 352
BstHHI GCGC 2 cut(s) 351, 937
BstKTI GATC 9 cut(s) 22, 42, 51, 303, 324, 360, 464, 549, 561
BstMAI GTCTC 3 cut(s) 67, 246, 467
BstMBI GATC 9 cut(s) 19, 39, 48, 300, 321, 357, 461, 546, 558
BstMCI CGRYCG 4 cut(s) 22, 157, 360, 495
BstNI CCWGG 1 cut(s) 59
BstNSI RCATGY 1 cut(s) 782
BstPAI GACNNNNGTC 1 cut(s) 443
BstSCI CCNGG 5 cut(s) 27, 33, 57, 290, 296
BstSLI GKGCMC 1 cut(s) 786
BstUI CGCG 4 cut(s) 159, 342, 356, 563
BstV1I GCAGC 3 cut(s) 74, 317, 930
BstX2I RGATCY 3 cut(s) 48, 300, 321
BstYI RGATCY 3 cut(s) 48, 300, 321
BstZI CGGCCG 1 cut(s) 492
BsuRI GGCC 3 cut(s) 494, 844, 953
BtsCI GGATG 5 cut(s) 231, 447, 771, 777, 889
BtsIMutI CAGTG 1 cut(s) 556
BtuMI TCGCGA 1 cut(s) 356
BveI ACCTGC 2 cut(s) 449, 475
Cac8I GCNNGC 3 cut(s) 521, 614, 806
CaiI CAGNNNCTG 1 cut(s) 943
CfoI GCGC 2 cut(s) 351, 937
Cfr10I RCCGGY 1 cut(s) 515
Cfr13I GGNCC 6 cut(s) 31, 294, 396, 438, 838, 843
CseI GACGC 5 cut(s) 148, 331, 433, 555, 835
CsiI ACCWGGT 1 cut(s) 57
CviAII CATG 2 cut(s) 779, 871
DdeI CTNAG 3 cut(s) 187, 501, 632
DpnI GATC 9 cut(s) 21, 41, 50, 302, 323, 359, 463, 548, 560
DpnII GATC 9 cut(s) 19, 39, 48, 300, 321, 357, 461, 546, 558
DrdI GACNNNNNNGTC 1 cut(s) 140
DseDI GACNNNNNNGTC 1 cut(s) 140
EaeI YGGCCR 1 cut(s) 492
EagI CGGCCG 1 cut(s) 492
EciI GGCGGA 3 cut(s) 208, 371, 662
Ecl136II GAGCTC 1 cut(s) 796
EclXI CGGCCG 1 cut(s) 492
Eco130I CCWWGG 1 cut(s) 451
Eco24I GRGCYC 4 cut(s) 507, 525, 618, 798
Eco31I GGTCTC 1 cut(s) 67
Eco47I GGWCC 5 cut(s) 31, 294, 396, 438, 838
Eco52I CGGCCG 1 cut(s) 492
Eco53kI GAGCTC 1 cut(s) 796
Eco88I CYCGRG 1 cut(s) 386
EcoICRI GAGCTC 1 cut(s) 796
EcoNI CCTNNNNNAGG 1 cut(s) 389
EcoRII CCWGG 1 cut(s) 57
EcoT14I CCWWGG 1 cut(s) 451
EcoT38I GRGCYC 4 cut(s) 507, 525, 618, 798
ErhI CCWWGG 1 cut(s) 451
FaeI CATG 2 cut(s) 782, 874
FaiI YATR 3 cut(s) 780, 872, 924
FalI AAGNNNNNCTT 2 cut(s) 792, 824
FaqI GGGAC 4 cut(s) 107, 409, 650, 667
FatI CATG 2 cut(s) 778, 870
FauI CCCGC 5 cut(s) 322, 512, 525, 605, 797
FblI GTMKAC 1 cut(s) 153
Fnu4HI GCNGC 5 cut(s) 88, 331, 564, 859, 944
FokI GGATG 5 cut(s) 218, 434, 778, 784, 896
FriOI GRGCYC 4 cut(s) 507, 525, 618, 798
Fsp4HI GCNGC 5 cut(s) 88, 331, 564, 859, 944
FspBI CTAG 4 cut(s) 140, 452, 747, 756
FspI TGCGCA 1 cut(s) 936
GlaI GCGC 2 cut(s) 350, 936
GluI GCNGC 5 cut(s) 88, 331, 564, 859, 944
GsuI CTGGAG 1 cut(s) 29
HaeII RGCGCY 1 cut(s) 352
HaeIII GGCC 3 cut(s) 494, 844, 953
HapII CCGG 9 cut(s) 29, 35, 292, 298, 372, 436, 495, 516, 841
HgaI GACGC 5 cut(s) 148, 331, 433, 555, 835
HhaI GCGC 2 cut(s) 351, 937
Hin1I GRCGYC 1 cut(s) 566
Hin1II CATG 2 cut(s) 782, 874
Hin6I GCGC 2 cut(s) 349, 935
HinP1I GCGC 2 cut(s) 349, 935
HincII GTYRAC 1 cut(s) 154
HindII GTYRAC 1 cut(s) 154
HinfI GANTC 5 cut(s) 134, 173, 179, 185, 475
HpaII CCGG 9 cut(s) 29, 35, 292, 298, 372, 436, 495, 516, 841
HphI GGTGA 6 cut(s) 49, 139, 499, 610, 673, 713
Hpy166II GTNNAC 2 cut(s) 154, 543
Hpy188I TCNGA 9 cut(s) 67, 172, 178, 184, 190, 461, 558, 633, 986
Hpy188III TCNNGA 5 cut(s) 46, 355, 388, 424, 436
Hpy8I GTNNAC 2 cut(s) 154, 543
Hpy99I CGWCG 7 cut(s) 155, 197, 200, 470, 484, 571, 829
HpyAV CCTTC 4 cut(s) 232, 385, 664, 692
HpyCH4III ACNGT 3 cut(s) 151, 400, 658
HpyCH4IV ACGT 1 cut(s) 198
HpyCH4V TGCA 1 cut(s) 741
HpyF3I CTNAG 3 cut(s) 187, 501, 632
HpySE526I ACGT 1 cut(s) 198
Hsp92I GRCGYC 1 cut(s) 566
Hsp92II CATG 2 cut(s) 782, 874
HspAI GCGC 2 cut(s) 349, 935
Kpn2I TCCGGA 1 cut(s) 435
Kzo9I GATC 9 cut(s) 19, 39, 48, 300, 321, 357, 461, 546, 558
LmnI GCTCC 4 cut(s) 30, 497, 604, 793
Lsp1109I GCAGC 3 cut(s) 74, 317, 930
MabI ACCWGGT 1 cut(s) 57
MaeI CTAG 4 cut(s) 140, 452, 747, 756
MaeII ACGT 1 cut(s) 198
MaeIII GTNAC 1 cut(s) 487
MalI GATC 9 cut(s) 21, 41, 50, 302, 323, 359, 463, 548, 560
MbiI CCGCTC 1 cut(s) 612
MboI GATC 9 cut(s) 19, 39, 48, 300, 321, 357, 461, 546, 558
MboII GAAGA 2 cut(s) 43, 707
MflI RGATCY 3 cut(s) 48, 300, 321
MhlI GDGCHC 6 cut(s) 507, 525, 556, 618, 786, 798
MluCI AATT 1 cut(s) 890
MlyI GAGTC 5 cut(s) 128, 167, 173, 179, 469
MmeI TCCRAC 5 cut(s) 195, 201, 207, 228, 711
MroI TCCGGA 1 cut(s) 435
MroXI GAANNNNTTC 2 cut(s) 107, 980
MseI TTAA 1 cut(s) 512
MslI CAYNNNNRTG 1 cut(s) 875
MspA1I CMGCKG 1 cut(s) 330
MspI CCGG 9 cut(s) 29, 35, 292, 298, 372, 436, 495, 516, 841
MspR9I CCNGG 5 cut(s) 29, 35, 59, 292, 298
MvaI CCWGG 1 cut(s) 59
MvnI CGCG 4 cut(s) 159, 342, 356, 563
NciI CCSGG 4 cut(s) 29, 35, 292, 298
NdeII GATC 9 cut(s) 19, 39, 48, 300, 321, 357, 461, 546, 558
NlaIII CATG 2 cut(s) 782, 874
NlaIV GGNNCC 6 cut(s) 26, 33, 296, 302, 397, 458
NmeAIII GCCGAG 1 cut(s) 442
NmuCI GTSAC 1 cut(s) 487
NruI TCGCGA 1 cut(s) 356
NsbI TGCGCA 1 cut(s) 936
NspI RCATGY 1 cut(s) 782
NspV TTCGAA 1 cut(s) 101
PaeR7I CTCGAG 1 cut(s) 386
PaqCI CACCTGC 1 cut(s) 475
PdmI GAANNNNTTC 2 cut(s) 107, 980
PflFI GACNNNGTC 3 cut(s) 59, 158, 478
PfoI TCCNGGA 1 cut(s) 27
PkrI GCNGC 5 cut(s) 89, 332, 565, 860, 945
Ple19I CGATCG 2 cut(s) 22, 360
PleI GAGTC 5 cut(s) 128, 167, 173, 179, 469
PpsI GAGTC 5 cut(s) 128, 167, 173, 179, 469
PshAI GACNNNNGTC 1 cut(s) 443
Psp124BI GAGCTC 1 cut(s) 798
Psp6I CCWGG 1 cut(s) 57
PspGI CCWGG 1 cut(s) 57
PspN4I GGNNCC 6 cut(s) 26, 33, 296, 302, 397, 458
PspPI GGNCC 6 cut(s) 31, 294, 396, 438, 838, 843
PstNI CAGNNNCTG 1 cut(s) 943
PsuI RGATCY 3 cut(s) 48, 300, 321
PsyI GACNNNGTC 3 cut(s) 59, 158, 478
PvuI CGATCG 2 cut(s) 22, 360
RruI TCGCGA 1 cut(s) 356
RseI CAYNNNNRTG 1 cut(s) 875
SacI GAGCTC 1 cut(s) 798
SalI GTCGAC 1 cut(s) 152
SaqAI TTAA 1 cut(s) 512
SatI GCNGC 5 cut(s) 88, 331, 564, 859, 944
Sau3AI GATC 9 cut(s) 19, 39, 48, 300, 321, 357, 461, 546, 558
Sau96I GGNCC 6 cut(s) 31, 294, 396, 438, 838, 843
SchI GAGTC 5 cut(s) 128, 167, 173, 179, 469
ScrFI CCNGG 5 cut(s) 29, 35, 59, 292, 298
SduI GDGCHC 6 cut(s) 507, 525, 556, 618, 786, 798
SexAI ACCWGGT 1 cut(s) 57
Sfr274I CTCGAG 1 cut(s) 386
SfuI TTCGAA 1 cut(s) 101
SinI GGWCC 5 cut(s) 31, 294, 396, 438, 838
SlaI CTCGAG 1 cut(s) 386
SmiMI CAYNNNNRTG 1 cut(s) 875
SmlI CTYRAG 5 cut(s) 236, 386, 404, 524, 947
SmoI CTYRAG 5 cut(s) 236, 386, 404, 524, 947
Sse9I AATT 1 cut(s) 890
SspMI CTAG 4 cut(s) 140, 452, 747, 756
SstI GAGCTC 1 cut(s) 798
StyD4I CCNGG 5 cut(s) 27, 33, 57, 290, 296
StyI CCWWGG 1 cut(s) 451
TaaI ACNGT 3 cut(s) 151, 400, 658
TaiI ACGT 1 cut(s) 201
TaqI TCGA 8 cut(s) 18, 54, 101, 153, 360, 387, 618, 816
TasI AATT 1 cut(s) 890
TauI GCSGC 2 cut(s) 566, 861
Tru1I TTAA 1 cut(s) 512
Tru9I TTAA 1 cut(s) 512
TscAI CASTG 1 cut(s) 556
TseFI GTSAC 1 cut(s) 487
TseI GCWGC 3 cut(s) 87, 330, 943
Tsp45I GTSAC 1 cut(s) 487
TspGWI ACGGA 2 cut(s) 454, 468
TspRI CASTG 1 cut(s) 556
Tth111I GACNNNGTC 3 cut(s) 59, 158, 478
VpaK11BI GGWCC 5 cut(s) 31, 294, 396, 438, 838
XagI CCTNNNNNAGG 1 cut(s) 389
XceI RCATGY 1 cut(s) 782
XhoI CTCGAG 1 cut(s) 386
XmaJI CCTAGG 1 cut(s) 451
XmiI GTMKAC 1 cut(s) 153
XmnI GAANNNNTTC 2 cut(s) 107, 980
XspI CTAG 4 cut(s) 140, 452, 747, 756
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.