MD15G1435200.v1.1

Spinocerebellar ataxia type 10 protein domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003909
Physical Location & Seq
Reverse (-)
53529833 .. 53533437
3605 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1435200.v1.1.491

Sequence Viewer

Length: 1482 bp
ATGGATAACACAGCACTGCAAGAGTGCTCTGTACCTGAAGATGTTCTCCGGAAAATTTTAAGTGTGGGAAACTCATCTACCCTCGTAAATTCGTTGGAAAATTTAATACAAGTTTGTAGAACTGCGGATGGTCGTGCAGGCCTTGCGTCTAAGGATATTCTTCCCTCTGTCATCCTGCTCATACAATCTCTCCCTTACCCTTCTGGTTGCCATCTTCTCACATTATGTTTAAAGCTCCTAAGAAATCTTTGTGCGGGAGAGATTGCAAATCAGGACTCATTTATTGAAAGAAATGGAGTGGCAATCATATTGAATGTTTTGAACTCCGCAAGTCTTTCTTCGGAGCCAGATACTGGGATCGTTCGGTTGGGGTTGCAAGTTCTCGCTAATGTTTCATTGGCTGGACAACGGCATCAGTGTGCAATTTGGCAGCAACTTTTTCCAAAAGAATTTGTTGCTATAGCAAGAGTTCAGAGCCGGGAAACTTGTGATCCCTTGTGCATGGTTATCTATGCTTGTTGTGATGGAAGCCCTGAACGGTTTGCCCAACTTTGCGGTGATCGTGGGATAACTATCGTGAAACAGATTGTAAAGACTACTGCTGCAGTTGGTTTCGGAGAAGATTGGTACAAGTTGATTCTTTCAAGAACCTGCTTAGAAGGACCTTACTTCCAGTCACTTTTCTCAAATTTAGGATTTGTTGGTGCTACTGAGAATGGCGAAGACACTGAATTTAGAGAAGACGTTTTCTCAACTGAGCAAGCATTTTTCTTGAGAAACATATCTGACATCTTGAATGAGCGGCTTCAAGAGATTACTGTGCCTAGTGATTTTGTACTGTGTGTCTTTGGGATATTTAAGAAATCTGCTGGGATTCTTAATTATGCCACAAGAGGCAAGTCTGGACTTCCAACAGGTTCTATTATGATAGATGTTCTAGGATACTCACTCACAATCCTGAGAGATGTATGTGCTCAGAAGTCCGTAAGAGGCTCTAACGAGGACCTGGGGGATGCTGTTGATGTTTTGCTGTCCCATGGCCTCATAGAATTACTTTTATGTTTGCTTCGTGATCTTGAGCCGACATCAGTGATGAGGAAAGCTATCAAGCAAGGTGAGGACCAAGATGGACCAAAGCCTTCTTCCTTAAAACCCTGCCCTTATAAAGGATTTCGGAGAGATATAGTGGCAGTCATTGGCAATTGCACATATAAAAGGAAGCTTGTACAAGATGAGATTAGACATAAGGATGGGATTCTTCTGCTCTTGCAACAGTGTGGCCTTGATGACGACAATCCGTACTTAAGGGAGTGGGGCATTTGGTGCGTGAGGAACTTATTCGAAGGCAACGAAGAAAACGAAAGGGTAGTGGCTGAATTGGAGCTTCAAGGGGCTGTTGATACACCAGAAATTGCTCAACTTGGTCTTCGAGTAGAGGTGAATCCAGAAACTAGACGGCCAAAGCTTGTTAACATCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Atx10homo_assoc

Protein Analysis

494

Amino Acids

54.41

Weight (kDa)

5.1

Isoelectric Point (pI)

45.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Atx10homo_assoc PF09759 391 - 484 2.5e-34 Spinocerebellar ataxia type 10 protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1164
Acc36I ACCTGC 1 cut(s) 659
AccB7I CCANNNNNTGG 1 cut(s) 353
AccBSI CCGCTC 1 cut(s) 802
AccIII TCCGGA 1 cut(s) 48
AciI CCGC 5 cut(s) 125, 254, 327, 555, 802
AclWI GGATC 2 cut(s) 365, 485
AcoI YGGCCR 1 cut(s) 1457
AcsI RAATTY 6 cut(s) 54, 88, 100, 449, 688, 731
AcuI CTGAAG 1 cut(s) 57
AfaI GTAC 5 cut(s) 33, 629, 837, 1227, 1301
AfiI CCNNNNNNNGG 2 cut(s) 353, 1166
AflII CTTAAG 1 cut(s) 1303
AgsI TTSAA 7 cut(s) 287, 313, 322, 645, 796, 809, 1388
AjnI CCWGG 1 cut(s) 1005
AjuI GAANNNNNNNTTGG 2 cut(s) 1126, 1158
AluBI AGCT 5 cut(s) 235, 1103, 1222, 1384, 1465
AluI AGCT 5 cut(s) 235, 1103, 1222, 1384, 1465
Alw21I GWGCWC 2 cut(s) 29, 976
AlwI GGATC 2 cut(s) 365, 485
AlwNI CAGNNNCTG 1 cut(s) 353
Aor13HI TCCGGA 1 cut(s) 48
AoxI GGCC 4 cut(s) 139, 1039, 1279, 1457
ApeKI GCWGC 2 cut(s) 430, 602
ApoI RAATTY 6 cut(s) 54, 88, 100, 449, 688, 731
Asp700I GAANNNNTTC 2 cut(s) 42, 1337
AspS9I GGNCC 4 cut(s) 662, 1003, 1120, 1130
AsuC2I CCSGG 1 cut(s) 479
AsuHPI GGTGA 3 cut(s) 569, 1127, 1450
AsuII TTCGAA 1 cut(s) 1341
AvaII GGWCC 4 cut(s) 662, 1003, 1120, 1130
BbsI GAAGAC 3 cut(s) 729, 747, 1418
Bbv12I GWGCWC 2 cut(s) 29, 976
BbvI GCAGC 2 cut(s) 442, 589
BccI CCATC 5 cut(s) 122, 219, 518, 1121, 1244
BceAI ACGGC 2 cut(s) 425, 1472
BciT130I CCWGG 1 cut(s) 1007
BciVI GTATCC 1 cut(s) 935
BcnI CCSGG 1 cut(s) 479
BfaI CTAG 3 cut(s) 825, 938, 1452
BfmI CTRYAG 2 cut(s) 459, 603
BfrI CTTAAG 1 cut(s) 1303
BfuAI ACCTGC 1 cut(s) 659
BfuI GTATCC 1 cut(s) 935
BisI GCNGC 3 cut(s) 431, 603, 803
BlsI GCNGC 3 cut(s) 432, 604, 804
Bme1390I CCNGG 2 cut(s) 479, 1007
Bme18I GGWCC 4 cut(s) 662, 1003, 1120, 1130
BmgT120I GGNCC 4 cut(s) 662, 1003, 1120, 1130
BmiI GGNNCC 1 cut(s) 345
BmrFI CCNGG 2 cut(s) 479, 1007
BmrI ACTGGG 1 cut(s) 363
BmsI GCATC 2 cut(s) 421, 1003
BmuI ACTGGG 1 cut(s) 363
BpiI GAAGAC 3 cut(s) 729, 747, 1418
Bpu14I TTCGAA 1 cut(s) 1341
BpuEI CTTGAG 2 cut(s) 793, 1097
BpuMI CCSGG 1 cut(s) 479
BsaBI GATNNNNATC 1 cut(s) 572
BsaJI CCNNGG 2 cut(s) 1006, 1036
BsaWI WCCGGW 1 cut(s) 48
BsaXI ACNNNNNCTCC 2 cut(s) 174, 204
Bsc4I CCNNNNNNNGG 2 cut(s) 353, 1166
Bse1I ACTGG 2 cut(s) 358, 673
Bse8I GATNNNNATC 1 cut(s) 572
BseAI TCCGGA 1 cut(s) 48
BseBI CCWGG 1 cut(s) 1007
BseDI CCNNGG 2 cut(s) 1006, 1036
BseGI GGATG 5 cut(s) 133, 171, 1018, 1255, 1473
BseJI GATNNNNATC 1 cut(s) 572
BseLI CCNNNNNNNGG 2 cut(s) 353, 1166
BseMII CTCAG 4 cut(s) 702, 747, 950, 989
BseNI ACTGG 2 cut(s) 358, 673
BseXI GCAGC 2 cut(s) 442, 589
BseYI CCCAGC 1 cut(s) 869
BsgI GTGCAG 1 cut(s) 156
BshFI GGCC 4 cut(s) 141, 1041, 1281, 1459
BsiHKAI GWGCWC 2 cut(s) 29, 976
BsiSI CCGG 2 cut(s) 49, 478
BslFI GGGAC 1 cut(s) 1018
BslI CCNNNNNNNGG 2 cut(s) 353, 1166
BsmFI GGGAC 1 cut(s) 1018
BsnI GGCC 4 cut(s) 141, 1041, 1281, 1459
Bsp119I TTCGAA 1 cut(s) 1341
Bsp1286I GDGCHC 2 cut(s) 29, 976
Bsp13I TCCGGA 1 cut(s) 48
Bsp1407I TGTACA 1 cut(s) 1225
Bsp143I GATC 4 cut(s) 357, 490, 559, 1072
Bsp19I CCATGG 1 cut(s) 1036
BspACI CCGC 5 cut(s) 125, 254, 327, 555, 802
BspANI GGCC 4 cut(s) 141, 1041, 1281, 1459
BspCNI CTCAG 4 cut(s) 703, 748, 951, 988
BspEI TCCGGA 1 cut(s) 48
BspLI GGNNCC 1 cut(s) 345
BspMAI CTGCAG 1 cut(s) 607
BspMI ACCTGC 1 cut(s) 659
BspPI GGATC 2 cut(s) 365, 485
BspT104I TTCGAA 1 cut(s) 1341
BspTI CTTAAG 1 cut(s) 1303
BsrBI CCGCTC 1 cut(s) 802
BsrGI TGTACA 1 cut(s) 1225
BsrI ACTGG 2 cut(s) 358, 673
BssECI CCNNGG 2 cut(s) 1006, 1036
BssMI GATC 4 cut(s) 357, 490, 559, 1072
BssT1I CCWWGG 1 cut(s) 1036
Bst2UI CCWGG 1 cut(s) 1007
Bst4CI ACNGT 4 cut(s) 540, 820, 840, 1275
BstAFI CTTAAG 1 cut(s) 1303
BstAPI GCANNNNNTGC 2 cut(s) 143, 1323
BstAUI TGTACA 1 cut(s) 1225
BstBI TTCGAA 1 cut(s) 1341
BstC8I GCNNGC 2 cut(s) 139, 762
BstDEI CTNAG 7 cut(s) 150, 239, 655, 711, 756, 959, 975
BstDSI CCRYGG 1 cut(s) 1036
BstENI CCTNNNNNAGG 1 cut(s) 1164
BstF5I GGATG 5 cut(s) 133, 171, 1018, 1255, 1473
BstKTI GATC 4 cut(s) 360, 493, 562, 1075
BstMBI GATC 4 cut(s) 357, 490, 559, 1072
BstMWI GCNNNNNNNGC 2 cut(s) 143, 1323
BstNI CCWGG 1 cut(s) 1007
BstSCI CCNGG 2 cut(s) 477, 1005
BstSFI CTRYAG 2 cut(s) 459, 603
BstV1I GCAGC 2 cut(s) 442, 589
BstV2I GAAGAC 3 cut(s) 729, 747, 1418
BsuI GTATCC 1 cut(s) 935
BsuRI GGCC 4 cut(s) 141, 1041, 1281, 1459
BtgI CCRYGG 1 cut(s) 1036
BtsCI GGATG 5 cut(s) 133, 171, 1018, 1255, 1473
BtsI GCAGTG 1 cut(s) 14
BtsIMutI CAGTG 5 cut(s) 14, 422, 726, 1095, 1280
BveI ACCTGC 1 cut(s) 659
Cac8I GCNNGC 2 cut(s) 139, 762
CaiI CAGNNNCTG 1 cut(s) 353
Cfr13I GGNCC 4 cut(s) 662, 1003, 1120, 1130
CseI GACGC 1 cut(s) 135
Csp6I GTAC 5 cut(s) 32, 628, 836, 1226, 1300
CviAII CATG 3 cut(s) 502, 1037, 1479
CviQI GTAC 5 cut(s) 32, 628, 836, 1226, 1300
DdeI CTNAG 7 cut(s) 150, 239, 655, 711, 756, 959, 975
DpnI GATC 4 cut(s) 359, 492, 561, 1074
DpnII GATC 4 cut(s) 357, 490, 559, 1072
DraI TTTAAA 1 cut(s) 231
EaeI YGGCCR 1 cut(s) 1457
Eco130I CCWWGG 1 cut(s) 1036
Eco147I AGGCCT 1 cut(s) 141
Eco47I GGWCC 4 cut(s) 662, 1003, 1120, 1130
Eco57I CTGAAG 1 cut(s) 57
EcoNI CCTNNNNNAGG 1 cut(s) 1164
EcoO109I RGGNCCY 2 cut(s) 662, 1003
EcoRII CCWGG 1 cut(s) 1005
EcoT14I CCWWGG 1 cut(s) 1036
ErhI CCWWGG 1 cut(s) 1036
FaeI CATG 3 cut(s) 505, 1040, 1482
FalI AAGNNNNNCTT 2 cut(s) 322, 354
FaqI GGGAC 1 cut(s) 1018
FatI CATG 3 cut(s) 501, 1036, 1478
FauI CCCGC 1 cut(s) 247
Fnu4HI GCNGC 3 cut(s) 431, 603, 803
FokI GGATG 5 cut(s) 140, 158, 1025, 1262, 1460
Fsp4HI GCNGC 3 cut(s) 431, 603, 803
FspBI CTAG 3 cut(s) 825, 938, 1452
GluI GCNGC 3 cut(s) 431, 603, 803
GsaI CCCAGC 1 cut(s) 873
HaeIII GGCC 4 cut(s) 141, 1041, 1281, 1459
HapII CCGG 2 cut(s) 49, 478
HgaI GACGC 1 cut(s) 135
Hin1II CATG 3 cut(s) 505, 1040, 1482
HincII GTYRAC 1 cut(s) 1471
HindII GTYRAC 1 cut(s) 1471
HindIII AAGCTT 2 cut(s) 1220, 1463
HinfI GANTC 5 cut(s) 275, 637, 874, 1255, 1441
HpaI GTTAAC 1 cut(s) 1471
HpaII CCGG 2 cut(s) 49, 478
HphI GGTGA 3 cut(s) 569, 1127, 1450
Hpy166II GTNNAC 1 cut(s) 1471
Hpy188I TCNGA 6 cut(s) 343, 474, 617, 787, 978, 1176
Hpy8I GTNNAC 1 cut(s) 1471
HpyAV CCTTC 4 cut(s) 210, 653, 1149, 1337
HpyCH4III ACNGT 4 cut(s) 540, 820, 840, 1275
HpyCH4IV ACGT 1 cut(s) 744
HpyCH4V TGCA 9 cut(s) 19, 137, 266, 376, 422, 501, 605, 1206, 1270
HpyF10VI GCNNNNNNNGC 2 cut(s) 143, 1323
HpyF3I CTNAG 7 cut(s) 150, 239, 655, 711, 756, 959, 975
HpySE526I ACGT 1 cut(s) 744
Hsp92II CATG 3 cut(s) 505, 1040, 1482
Kpn2I TCCGGA 1 cut(s) 48
KspAI GTTAAC 1 cut(s) 1471
Kzo9I GATC 4 cut(s) 357, 490, 559, 1072
LmnI GCTCC 3 cut(s) 240, 343, 1381
Lsp1109I GCAGC 2 cut(s) 442, 589
LweI GCATC 2 cut(s) 421, 1003
MaeI CTAG 3 cut(s) 825, 938, 1452
MaeII ACGT 1 cut(s) 744
MaeIII GTNAC 1 cut(s) 675
MalI GATC 4 cut(s) 359, 492, 561, 1074
MbiI CCGCTC 1 cut(s) 802
MboI GATC 4 cut(s) 357, 490, 559, 1072
MfeI CAATTG 1 cut(s) 1201
MhlI GDGCHC 2 cut(s) 29, 976
MlyI GAGTC 1 cut(s) 269
MmeI TCCRAC 2 cut(s) 75, 935
MroI TCCGGA 1 cut(s) 48
MroXI GAANNNNTTC 2 cut(s) 42, 1337
MseI TTAA 8 cut(s) 59, 104, 230, 858, 879, 1148, 1304, 1470
MslI CAYNNNNRTG 2 cut(s) 417, 1248
MspCI CTTAAG 1 cut(s) 1303
MspI CCGG 2 cut(s) 49, 478
MspR9I CCNGG 2 cut(s) 479, 1007
MunI CAATTG 1 cut(s) 1201
MvaI CCWGG 1 cut(s) 1007
MwoI GCNNNNNNNGC 2 cut(s) 143, 1323
NciI CCSGG 1 cut(s) 479
NcoI CCATGG 1 cut(s) 1036
NdeII GATC 4 cut(s) 357, 490, 559, 1072
NlaIII CATG 3 cut(s) 505, 1040, 1482
NlaIV GGNNCC 1 cut(s) 345
NmuCI GTSAC 1 cut(s) 675
NspV TTCGAA 1 cut(s) 1341
PceI AGGCCT 1 cut(s) 141
PcsI WCGNNNNNNNCGW 2 cut(s) 1347, 1356
PdmI GAANNNNTTC 2 cut(s) 42, 1337
PfeI GAWTC 4 cut(s) 637, 874, 1255, 1441
PflMI CCANNNNNTGG 1 cut(s) 353
PkrI GCNGC 3 cut(s) 432, 604, 804
PleI GAGTC 1 cut(s) 269
PpsI GAGTC 1 cut(s) 269
PpuMI RGGWCCY 2 cut(s) 662, 1003
PsiI TTATAA 1 cut(s) 1164
Psp5II RGGWCCY 2 cut(s) 662, 1003
Psp6I CCWGG 1 cut(s) 1005
PspFI CCCAGC 1 cut(s) 869
PspGI CCWGG 1 cut(s) 1005
PspN4I GGNNCC 1 cut(s) 345
PspPI GGNCC 4 cut(s) 662, 1003, 1120, 1130
PspPPI RGGWCCY 2 cut(s) 662, 1003
PstI CTGCAG 1 cut(s) 607
PstNI CAGNNNCTG 1 cut(s) 353
RsaI GTAC 5 cut(s) 33, 629, 837, 1227, 1301
RsaNI GTAC 5 cut(s) 32, 628, 836, 1226, 1300
RseI CAYNNNNRTG 2 cut(s) 417, 1248
SaqAI TTAA 8 cut(s) 59, 104, 230, 858, 879, 1148, 1304, 1470
SatI GCNGC 3 cut(s) 431, 603, 803
Sau3AI GATC 4 cut(s) 357, 490, 559, 1072
Sau96I GGNCC 4 cut(s) 662, 1003, 1120, 1130
SchI GAGTC 1 cut(s) 269
ScrFI CCNGG 2 cut(s) 479, 1007
SduI GDGCHC 2 cut(s) 29, 976
SfaNI GCATC 2 cut(s) 421, 1003
SfcI CTRYAG 2 cut(s) 459, 603
SfuI TTCGAA 1 cut(s) 1341
SinI GGWCC 4 cut(s) 662, 1003, 1120, 1130
SmiMI CAYNNNNRTG 2 cut(s) 417, 1248
SmlI CTYRAG 3 cut(s) 772, 1076, 1303
SmoI CTYRAG 3 cut(s) 772, 1076, 1303
SseBI AGGCCT 1 cut(s) 141
SsiI CCGC 5 cut(s) 125, 254, 327, 555, 802
SspMI CTAG 3 cut(s) 825, 938, 1452
StuI AGGCCT 1 cut(s) 141
StyD4I CCNGG 2 cut(s) 477, 1005
StyI CCWWGG 1 cut(s) 1036
TaaI ACNGT 4 cut(s) 540, 820, 840, 1275
TaiI ACGT 1 cut(s) 747
TaqI TCGA 2 cut(s) 1341, 1429
TatI WGTACW 2 cut(s) 835, 1225
TauI GCSGC 1 cut(s) 805
TfiI GAWTC 4 cut(s) 637, 874, 1255, 1441
Tru1I TTAA 8 cut(s) 59, 104, 230, 858, 879, 1148, 1304, 1470
Tru9I TTAA 8 cut(s) 59, 104, 230, 858, 879, 1148, 1304, 1470
TscAI CASTG 5 cut(s) 21, 422, 733, 1095, 1280
TseFI GTSAC 1 cut(s) 675
TseI GCWGC 2 cut(s) 430, 602
Tsp45I GTSAC 1 cut(s) 675
TspDTI ATGAA 1 cut(s) 384
TspGWI ACGGA 2 cut(s) 973, 1287
TspRI CASTG 5 cut(s) 21, 422, 733, 1095, 1280
Van91I CCANNNNNTGG 1 cut(s) 353
Vha464I CTTAAG 1 cut(s) 1303
VpaK11BI GGWCC 4 cut(s) 662, 1003, 1120, 1130
XagI CCTNNNNNAGG 1 cut(s) 1164
XapI RAATTY 6 cut(s) 54, 88, 100, 449, 688, 731
XmnI GAANNNNTTC 2 cut(s) 42, 1337
XspI CTAG 3 cut(s) 825, 938, 1452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.