MD16G1094100.v1.1

Glycosyl hydrolases family 17

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0009684
Physical Location & Seq
Forward (+)
6506802 .. 6507242
441 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1094100.v1.1.491

Sequence Viewer

Length: 441 bp
ATGGTCAACTCCTACCCCTACTTTAGCTACCTACGCAACAAGAACTACGTCTCTCTTGAATACGCGTTGTTTCGAGCCACCAGTGAGGTCGTTAACGATGGTGAGTTGGTGTATAGTAATCTTTTCGACGCGAGTGTGGATGCTTTTGTGAGCGCGATGGAGAGGGAGGGAGTTGTTGGAGTTCCCGTGGTGGTTTCGGAGACGGGGTGGCCGACCGGCGGAGGGGAGGCCGCTAGTGTGGAGAATTCGAGGGCATACAATGCGGAGGTGGTGAGACGGGCGGTGGAAGGGGTTGGGACGCCGAGGAGGCCGGGGGTTGGAGTGGAGGTTTTCTTGTTTGATTTGTTTGATGAGAATGAGAAGCATGGGGAAGAGTTTGAGAGACATTTCGGGATTTTTGGGCCTGATGGGCTCAAGGCTTATGACCTTAATTTCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_17

Protein Analysis

147

Amino Acids

16.18

Weight (kDa)

4.53

Isoelectric Point (pI)

30.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 1 - 145 2.2e-44 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 65, 131, 155
AciI CCGC 4 cut(s) 219, 231, 263, 281
AcoI YGGCCR 1 cut(s) 209
AcsI RAATTY 1 cut(s) 244
AcyI GRCGYC 1 cut(s) 299
AfiI CCNNNNNNNGG 3 cut(s) 218, 222, 317
AflIII ACRYGT 1 cut(s) 63
AgsI TTSAA 2 cut(s) 59, 436
AluBI AGCT 1 cut(s) 27
AluI AGCT 1 cut(s) 27
Alw26I GTCTC 4 cut(s) 55, 194, 268, 376
AoxI GGCC 4 cut(s) 209, 228, 308, 401
ApoI RAATTY 1 cut(s) 244
AspLEI GCGC 1 cut(s) 155
AspS9I GGNCC 1 cut(s) 401
AsuC2I CCSGG 1 cut(s) 312
AsuHPI GGTGA 2 cut(s) 113, 283
BanII GRGCYC 1 cut(s) 414
BccI CCATC 3 cut(s) 92, 151, 401
BcnI CCSGG 1 cut(s) 312
BcoDI GTCTC 4 cut(s) 55, 194, 268, 376
BfaI CTAG 1 cut(s) 234
BglI GCCNNNNNGGC 2 cut(s) 307, 409
BisI GCNGC 1 cut(s) 231
BlsI GCNGC 1 cut(s) 232
Bme1390I CCNGG 1 cut(s) 312
BmgT120I GGNCC 1 cut(s) 401
BmrFI CCNGG 1 cut(s) 312
BmsI GCATC 1 cut(s) 130
BpuEI CTTGAG 1 cut(s) 398
BpuMI CCSGG 1 cut(s) 312
BsaHI GRCGYC 1 cut(s) 299
BsaJI CCNNGG 3 cut(s) 186, 302, 311
BsaXI ACNNNNNCTCC 6 cut(s) 171, 191, 201, 221, 312, 342
Bsc4I CCNNNNNNNGG 3 cut(s) 218, 222, 317
Bse118I RCCGGY 1 cut(s) 215
Bse1I ACTGG 1 cut(s) 81
BseDI CCNNGG 3 cut(s) 186, 302, 311
BseGI GGATG 1 cut(s) 145
BseLI CCNNNNNNNGG 3 cut(s) 218, 222, 317
BseNI ACTGG 1 cut(s) 81
BseRI GAGGAG 1 cut(s) 319
Bsh1236I CGCG 3 cut(s) 65, 131, 155
Bsh1285I CGRYCG 1 cut(s) 216
BshFI GGCC 4 cut(s) 211, 230, 310, 403
BsiEI CGRYCG 1 cut(s) 216
BsiSI CCGG 2 cut(s) 216, 311
BslFI GGGAC 1 cut(s) 310
BslI CCNNNNNNNGG 3 cut(s) 218, 222, 317
BsmAI GTCTC 4 cut(s) 55, 194, 268, 376
BsmBI CGTCTC 3 cut(s) 55, 194, 268
BsmFI GGGAC 1 cut(s) 310
BsnI GGCC 4 cut(s) 211, 230, 310, 403
Bsp1286I GDGCHC 1 cut(s) 414
BspACI CCGC 4 cut(s) 219, 231, 263, 281
BspANI GGCC 4 cut(s) 211, 230, 310, 403
BspFNI CGCG 3 cut(s) 65, 131, 155
BsrFI RCCGGY 1 cut(s) 215
BsrI ACTGG 1 cut(s) 81
BssAI RCCGGY 1 cut(s) 215
BssECI CCNNGG 3 cut(s) 186, 302, 311
BssNI GRCGYC 1 cut(s) 299
Bst6I CTCTTC 1 cut(s) 366
BstACI GRCGYC 1 cut(s) 299
BstAPI GCANNNNNTGC 1 cut(s) 260
BstDSI CCRYGG 1 cut(s) 186
BstF5I GGATG 1 cut(s) 145
BstFNI CGCG 3 cut(s) 65, 131, 155
BstHHI GCGC 1 cut(s) 155
BstMAI GTCTC 4 cut(s) 55, 194, 268, 376
BstMCI CGRYCG 1 cut(s) 216
BstMWI GCNNNNNNNGC 4 cut(s) 33, 260, 307, 409
BstSCI CCNGG 1 cut(s) 310
BstUI CGCG 3 cut(s) 65, 131, 155
BsuRI GGCC 4 cut(s) 211, 230, 310, 403
BtgI CCRYGG 1 cut(s) 186
BtgZI GCGATG 1 cut(s) 170
BtsCI GGATG 1 cut(s) 145
BtsIMutI CAGTG 1 cut(s) 88
CfoI GCGC 1 cut(s) 155
Cfr10I RCCGGY 1 cut(s) 215
Cfr13I GGNCC 1 cut(s) 401
CseI GACGC 2 cut(s) 137, 307
CviAII CATG 1 cut(s) 365
CviJI RGCY 8 cut(s) 27, 77, 211, 230, 310, 403, 412, 419
CviKI_1 RGCY 8 cut(s) 27, 77, 211, 230, 310, 403, 412, 419
EaeI YGGCCR 1 cut(s) 209
Eam1104I CTCTTC 1 cut(s) 366
EarI CTCTTC 1 cut(s) 366
EciI GGCGGA 1 cut(s) 234
Eco24I GRGCYC 1 cut(s) 414
EcoRI GAATTC 1 cut(s) 244
EcoT38I GRGCYC 1 cut(s) 414
Esp3I CGTCTC 3 cut(s) 55, 194, 268
FaeI CATG 1 cut(s) 368
FaiI YATR 4 cut(s) 114, 256, 366, 423
FaqI GGGAC 1 cut(s) 310
FatI CATG 1 cut(s) 364
Fnu4HI GCNGC 1 cut(s) 231
FokI GGATG 1 cut(s) 152
FriOI GRGCYC 1 cut(s) 414
Fsp4HI GCNGC 1 cut(s) 231
FspBI CTAG 1 cut(s) 234
GlaI GCGC 1 cut(s) 154
GluI GCNGC 1 cut(s) 231
HaeIII GGCC 4 cut(s) 211, 230, 310, 403
HapII CCGG 2 cut(s) 216, 311
HgaI GACGC 2 cut(s) 137, 307
HhaI GCGC 1 cut(s) 155
Hin1I GRCGYC 1 cut(s) 299
Hin1II CATG 1 cut(s) 368
Hin6I GCGC 1 cut(s) 153
HinP1I GCGC 1 cut(s) 153
HincII GTYRAC 2 cut(s) 7, 94
HindII GTYRAC 2 cut(s) 7, 94
HpaI GTTAAC 1 cut(s) 94
HpaII CCGG 2 cut(s) 216, 311
HphI GGTGA 2 cut(s) 113, 283
Hpy166II GTNNAC 2 cut(s) 7, 94
Hpy188I TCNGA 1 cut(s) 199
Hpy188III TCNNGA 2 cut(s) 56, 391
Hpy8I GTNNAC 2 cut(s) 7, 94
Hpy99I CGWCG 1 cut(s) 131
HpyAV CCTTC 1 cut(s) 281
HpyCH4IV ACGT 1 cut(s) 48
HpyF10VI GCNNNNNNNGC 4 cut(s) 33, 260, 307, 409
HpySE526I ACGT 1 cut(s) 48
Hsp92I GRCGYC 1 cut(s) 299
Hsp92II CATG 1 cut(s) 368
HspAI GCGC 1 cut(s) 153
KspAI GTTAAC 1 cut(s) 94
LpnPI CCDG 4 cut(s) 94, 229, 324, 417
LweI GCATC 1 cut(s) 130
MaeI CTAG 1 cut(s) 234
MaeII ACGT 1 cut(s) 48
MboII GAAGA 1 cut(s) 383
MhlI GDGCHC 1 cut(s) 414
MluCI AATT 2 cut(s) 244, 430
MluI ACGCGT 1 cut(s) 63
MmeI TCCRAC 2 cut(s) 157, 298
MseI TTAA 2 cut(s) 93, 429
MspI CCGG 2 cut(s) 216, 311
MspR9I CCNGG 1 cut(s) 312
MvnI CGCG 3 cut(s) 65, 131, 155
MwoI GCNNNNNNNGC 4 cut(s) 33, 260, 307, 409
NciI CCSGG 1 cut(s) 312
NlaIII CATG 1 cut(s) 368
NmeAIII GCCGAG 1 cut(s) 327
PcsI WCGNNNNNNNCGW 1 cut(s) 209
PkrI GCNGC 1 cut(s) 232
PspPI GGNCC 1 cut(s) 401
SaqAI TTAA 2 cut(s) 93, 429
SatI GCNGC 1 cut(s) 231
Sau96I GGNCC 1 cut(s) 401
ScrFI CCNGG 1 cut(s) 312
SduI GDGCHC 1 cut(s) 414
SetI ASST 7 cut(s) 29, 33, 51, 90, 270, 330, 429
SfaNI GCATC 1 cut(s) 130
SmlI CTYRAG 1 cut(s) 413
SmoI CTYRAG 1 cut(s) 413
Sse9I AATT 2 cut(s) 244, 430
SsiI CCGC 4 cut(s) 219, 231, 263, 281
SspMI CTAG 1 cut(s) 234
StyD4I CCNGG 1 cut(s) 310
TaiI ACGT 1 cut(s) 51
TaqI TCGA 3 cut(s) 73, 126, 248
TasI AATT 2 cut(s) 244, 430
TauI GCSGC 1 cut(s) 233
Tru1I TTAA 2 cut(s) 93, 429
Tru9I TTAA 2 cut(s) 93, 429
TscAI CASTG 1 cut(s) 88
TspRI CASTG 1 cut(s) 88
XapI RAATTY 1 cut(s) 244
XspI CTAG 1 cut(s) 234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.