MD16G1094300.v1.1

Glycosyl hydrolases family 17

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0009684
Physical Location & Seq
Forward (+)
6528035 .. 6528475
441 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1094300.v1.1.491

Sequence Viewer

Length: 441 bp
ATGGTCAACTCCTACCCCTACTTTAGCTACCTACACAACAAGAACTACGTCTCTCTTGGATACGCGTTGTTTCGAGCCACCAATGAGGTGGTTAACGACGACGAGTTGGTGTATAGTAATCTTTTCGACGCGAGTATGGATGATTTTGTGAGTGTGATGGAAAGGGAGAGAGTTGTTGGAGTTCCCGTGGTGGTTTCGGAGATGGGGTGGCCGACCGACGGAGGGGAGGCCGTTAGTGTGGAGAATTCGAGGGCGTACAATGCGGAGGTGGTGAGACGGGCGGTGGAAGGGGTTGGGACGCCAAGGAGGCCGGGAGTTGGAGCGGAGGTTTTCTTGTTTGATTTGTTTGATGAGAATGAGAAGTATGGGGAAGAATTTGAGAGGCATTTCGGGATTTTTGGGCCTGACGGGCTCAAGGCTTATGACCTTAATTTCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_17

Protein Analysis

147

Amino Acids

16.49

Weight (kDa)

4.41

Isoelectric Point (pI)

28.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 1 - 145 1.7e-38 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 323
AccII CGCG 2 cut(s) 65, 131
AciI CCGC 3 cut(s) 263, 281, 323
AcoI YGGCCR 1 cut(s) 209
AcsI RAATTY 2 cut(s) 244, 374
AcyI GRCGYC 1 cut(s) 299
AfaI GTAC 1 cut(s) 257
AfiI CCNNNNNNNGG 3 cut(s) 218, 222, 317
AflIII ACRYGT 1 cut(s) 63
AgsI TTSAA 1 cut(s) 436
AluBI AGCT 1 cut(s) 27
AluI AGCT 1 cut(s) 27
Alw26I GTCTC 2 cut(s) 55, 268
AoxI GGCC 4 cut(s) 209, 228, 308, 401
ApoI RAATTY 2 cut(s) 244, 374
AspS9I GGNCC 1 cut(s) 401
AsuC2I CCSGG 1 cut(s) 312
AsuHPI GGTGA 1 cut(s) 283
BanII GRGCYC 1 cut(s) 414
BccI CCATC 2 cut(s) 151, 196
BceAI ACGGC 1 cut(s) 215
BciVI GTATCC 1 cut(s) 53
BcnI CCSGG 1 cut(s) 312
BcoDI GTCTC 2 cut(s) 55, 268
BfuI GTATCC 1 cut(s) 53
BglI GCCNNNNNGGC 2 cut(s) 307, 409
Bme1390I CCNGG 1 cut(s) 312
BmgT120I GGNCC 1 cut(s) 401
BmrFI CCNGG 1 cut(s) 312
BpuEI CTTGAG 1 cut(s) 398
BpuMI CCSGG 1 cut(s) 312
BsaHI GRCGYC 1 cut(s) 299
BsaJI CCNNGG 2 cut(s) 186, 302
BsaXI ACNNNNNCTCC 6 cut(s) 171, 191, 201, 221, 312, 342
Bsc4I CCNNNNNNNGG 3 cut(s) 218, 222, 317
BseDI CCNNGG 2 cut(s) 186, 302
BseGI GGATG 1 cut(s) 145
BseLI CCNNNNNNNGG 3 cut(s) 218, 222, 317
Bsh1236I CGCG 2 cut(s) 65, 131
Bsh1285I CGRYCG 1 cut(s) 216
BshFI GGCC 4 cut(s) 211, 230, 310, 403
BsiEI CGRYCG 1 cut(s) 216
BsiSI CCGG 1 cut(s) 311
BslFI GGGAC 1 cut(s) 310
BslI CCNNNNNNNGG 3 cut(s) 218, 222, 317
BsmAI GTCTC 2 cut(s) 55, 268
BsmBI CGTCTC 2 cut(s) 55, 268
BsmFI GGGAC 1 cut(s) 310
BsnI GGCC 4 cut(s) 211, 230, 310, 403
Bsp1286I GDGCHC 1 cut(s) 414
BspACI CCGC 3 cut(s) 263, 281, 323
BspANI GGCC 4 cut(s) 211, 230, 310, 403
BspFNI CGCG 2 cut(s) 65, 131
BsrBI CCGCTC 1 cut(s) 323
BssECI CCNNGG 2 cut(s) 186, 302
BssNI GRCGYC 1 cut(s) 299
BssT1I CCWWGG 1 cut(s) 302
BstACI GRCGYC 1 cut(s) 299
BstDSI CCRYGG 1 cut(s) 186
BstF5I GGATG 1 cut(s) 145
BstFNI CGCG 2 cut(s) 65, 131
BstMAI GTCTC 2 cut(s) 55, 268
BstMCI CGRYCG 1 cut(s) 216
BstMWI GCNNNNNNNGC 3 cut(s) 260, 307, 409
BstSCI CCNGG 1 cut(s) 310
BstUI CGCG 2 cut(s) 65, 131
BstXI CCANNNNNNTGG 1 cut(s) 88
BsuI GTATCC 1 cut(s) 53
BsuRI GGCC 4 cut(s) 211, 230, 310, 403
BtgI CCRYGG 1 cut(s) 186
BtsCI GGATG 1 cut(s) 145
Cfr13I GGNCC 1 cut(s) 401
CseI GACGC 2 cut(s) 137, 307
Csp6I GTAC 1 cut(s) 256
CviJI RGCY 8 cut(s) 27, 77, 211, 230, 310, 403, 412, 419
CviKI_1 RGCY 8 cut(s) 27, 77, 211, 230, 310, 403, 412, 419
CviQI GTAC 1 cut(s) 256
EaeI YGGCCR 1 cut(s) 209
Eco130I CCWWGG 1 cut(s) 302
Eco24I GRGCYC 1 cut(s) 414
EcoRI GAATTC 1 cut(s) 244
EcoT14I CCWWGG 1 cut(s) 302
EcoT38I GRGCYC 1 cut(s) 414
ErhI CCWWGG 1 cut(s) 302
Esp3I CGTCTC 2 cut(s) 55, 268
FaiI YATR 4 cut(s) 114, 137, 366, 423
FaqI GGGAC 1 cut(s) 310
FokI GGATG 1 cut(s) 152
FriOI GRGCYC 1 cut(s) 414
HaeIII GGCC 4 cut(s) 211, 230, 310, 403
HapII CCGG 1 cut(s) 311
HgaI GACGC 2 cut(s) 137, 307
Hin1I GRCGYC 1 cut(s) 299
HincII GTYRAC 2 cut(s) 7, 94
HindII GTYRAC 2 cut(s) 7, 94
HpaI GTTAAC 1 cut(s) 94
HpaII CCGG 1 cut(s) 311
HphI GGTGA 1 cut(s) 283
Hpy166II GTNNAC 2 cut(s) 7, 94
Hpy188I TCNGA 1 cut(s) 199
Hpy188III TCNNGA 1 cut(s) 391
Hpy8I GTNNAC 2 cut(s) 7, 94
Hpy99I CGWCG 4 cut(s) 101, 104, 131, 221
HpyAV CCTTC 1 cut(s) 281
HpyCH4IV ACGT 1 cut(s) 48
HpyF10VI GCNNNNNNNGC 3 cut(s) 260, 307, 409
HpySE526I ACGT 1 cut(s) 48
Hsp92I GRCGYC 1 cut(s) 299
KspAI GTTAAC 1 cut(s) 94
LmnI GCTCC 1 cut(s) 320
LpnPI CCDG 2 cut(s) 324, 417
MaeII ACGT 1 cut(s) 48
MbiI CCGCTC 1 cut(s) 323
MboII GAAGA 1 cut(s) 383
MhlI GDGCHC 1 cut(s) 414
MluCI AATT 3 cut(s) 244, 374, 430
MluI ACGCGT 1 cut(s) 63
MmeI TCCRAC 2 cut(s) 157, 298
MnlI CCTC 8 cut(s) 79, 215, 220, 243, 259, 300, 319, 375
MseI TTAA 2 cut(s) 93, 429
MspI CCGG 1 cut(s) 311
MspR9I CCNGG 1 cut(s) 312
MvnI CGCG 2 cut(s) 65, 131
MwoI GCNNNNNNNGC 3 cut(s) 260, 307, 409
NciI CCSGG 1 cut(s) 312
PspPI GGNCC 1 cut(s) 401
RsaI GTAC 1 cut(s) 257
RsaNI GTAC 1 cut(s) 256
SaqAI TTAA 2 cut(s) 93, 429
Sau96I GGNCC 1 cut(s) 401
ScrFI CCNGG 1 cut(s) 312
SduI GDGCHC 1 cut(s) 414
SetI ASST 7 cut(s) 29, 33, 51, 90, 270, 330, 429
SmlI CTYRAG 1 cut(s) 413
SmoI CTYRAG 1 cut(s) 413
Sse9I AATT 3 cut(s) 244, 374, 430
SsiI CCGC 3 cut(s) 263, 281, 323
StyD4I CCNGG 1 cut(s) 310
StyI CCWWGG 1 cut(s) 302
TaiI ACGT 1 cut(s) 51
TaqI TCGA 3 cut(s) 73, 126, 248
TaqII GACCGA 1 cut(s) 230
TasI AATT 3 cut(s) 244, 374, 430
Tru1I TTAA 2 cut(s) 93, 429
Tru9I TTAA 2 cut(s) 93, 429
TspGWI ACGGA 1 cut(s) 234
XapI RAATTY 2 cut(s) 244, 374
XcmI CCANNNNNNNNNTGG 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.