Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0006982

Physical Location & Seq

Genomic Coordinates Forward (+)

7815415 .. 7818468

Gene Structure 3054 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1111300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1317 bp

ATGAAACAATCGTCGAAGGAAGCTGAAGAGGCGAGGCAGAAGCAGATTGACGATTGGCTTCCGGTCACTTCTGCTAGGAATGCCAAATGGTGGTACTCAGCTTTCCACAATGTCACTGCCATGGTTGGGGCTGGTGTCCTTAGCCTTCCCTATGCCATGGCCGAGCTTGGATGGGGTCCTGGCATTGCTGTCGTGCTTCTGTCATGGATCATCACCCTATATACACTATGGCAAATGGTTGAGATGCATGAAATGGTGCCGGGGAAGCGGTTCGATCGGTACCACGAGCTGGGACAGCATGCCTTTGGAGAGAAGCTGGGCCTCTGGGTCGTTGTGCCCCAACAGCTAATTGTGGAAGTTGGTGTCAATATTGTCTATATGGTCACAGGAGGAAAGTCATTGAAGAAGGTCCATGATGTGATTTGCCCTAGCTGCAAAGACATCAAACTCACCTTATGGATTTTGATTTTTGCTTCTGCCCACTTTGTTCTCTCCCATCTCCCCAACTTAAACTCCGTCTCTGGTATCTCGCTTGCTGCCGCAGTCATGTCCTTGAGTTACTCAACGATTGCGTGGGGAGCTGCTATTCACAAGGGGAAGGTACCGAACGTGGACTACAGCTACAAAGCTAAATCCAATGTCGGAGCTGTCTTCAACTTCTTTAGTGGGTTGGGAGACATAGCATTTGCCTACGCTGGCCACAATGTCGTTTTGGAGATCCAAGCAACGATTCCTTCCACACCCGAGAAGCCTTCCAAGGGACCAATGTGGAAAGGAGTTGTTGTGGCATACATTGTGGTGGCTTTGTGCTACTTCCCTGTTTGTTTTGTTGGCTACTATATTTACGGAAACTCTGTTGAAGACAACATCCTCATCTCACTCGAAAAACCTGTCTGGCTAATTGCAGCAGCTAACATATTTGTTGTGATCCATGTCATTGGAAGCTACCAAGTTTTCGCGATGGGAGTTTTTGACATGGTTGAAACTTTTCTGGTCAAGAAAATGAACTTCCCGCCTTCTTTCACCCTTCGCTTTATTACCCGCACGATATATGTTGCAATTACCATGGTCATTGGTATGGCCATCCCTTTCTTTGGTGGCCTTCTCGGATTCTTTGGAGGATTTGCTTTCGCCCCAACCACATACTTCCTTCCCTGCATCATGTGGCTCGCCATCTACAAACCGAGACGTTTCAGTCTATCTTGGTGCATAAACTGGACCTGCATTATACTGGGAGCTACCTTGATGATTCTAGCACCCATCGGTGCCCTACGGAGTATCATCTTGCAATCGAAAACCTACACATTCTTCTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003333 GO:0003674 GO:0005215 GO:0005342 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006865 GO:0008150 GO:0008509 GO:0008514 GO:0015075 GO:0015171 GO:0015318 GO:0015711 GO:0015849 GO:0016020 GO:0022857 GO:0034220 GO:0044464 GO:0046942 GO:0046943 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0071944 GO:0098656 GO:1903825 GO:1905039

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Aa_trans

Protein Analysis

439

Amino Acids

48.62

Weight (kDa)

9.06

Isoelectric Point (pI)

38.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Aa_trans	PF01490	27 - 423	3.1e-106	Transmembrane amino acid transporter protein
Trp_Tyr_perm	PF03222	32 - 142	1e-07	Tryptophan/tyrosine permease family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0006982)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G24400 AT1G67640
fragaria_vesca	FvH4_4g24381 FvH4_4g24381 FvH4_4g24390
malus_domestica	MD13G1111700.v1.1 MD16G1111300.v1.1
prunus_persica	Prupe.1G233800_v2.0.a1
pyrus_communis	pycom16g09430
rosa_chinensis	RchiOBHm_Chr4g0431231 RchiOBHm_Chr4g0431241
rosa_laevigata	RLG00000006916 RLG00000006917
rosa_multiflora	Rmu_sc0005258.1_g000008 Rmu_sc0039160.1_g000001
rosa_roxburghii	Rroxscaffold_5G00372770 Rroxscaffold_5G00372780
rosa_rugosa	Rorug04G0249400 Rorug04G0249500 Rorug04G0249600
rosa_samantha	Rh4AG305500 Rh4BG312800 Rh4BG312900
rosa_wichuraiana	Rw4G026550 Rw4G026560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1194
Acc36I	ACCTGC	1 cut(s)	1229
Acc65I	GGTACC	2 cut(s)	279, 601
AccB1I	GGYRCC	4 cut(s)	256, 279, 601, 1265
AccB7I	CCANNNNNTGG	2 cut(s)	90, 289
AccII	CGCG	1 cut(s)	959
AciI	CCGC	4 cut(s)	268, 540, 1013, 1042
AclWI	GGATC	3 cut(s)	215, 712, 922
AcoI	YGGCCR	3 cut(s)	159, 697, 1080
AcuI	CTGAAG	1 cut(s)	45
AfaI	GTAC	3 cut(s)	95, 281, 603
AfiI	CCNNNNNNNGG	5 cut(s)	90, 126, 289, 758, 1094
AgsI	TTSAA	4 cut(s)	403, 655, 860, 983
AjnI	CCWGG	1 cut(s)	178
AjuI	GAANNNNNNNTTGG	2 cut(s)	714, 746
Alw26I	GTCTC	3 cut(s)	523, 669, 1180
AlwI	GGATC	3 cut(s)	215, 712, 922
Ama87I	CYCGRG	1 cut(s)	743
AoxI	GGCC	5 cut(s)	159, 319, 697, 1080, 1099
ApeKI	GCWGC	5 cut(s)	432, 536, 581, 905, 908
ArsI	GACNNNNNNTTYG	4 cut(s)	668, 700, 876, 908
Asp700I	GAANNNNTTC	2 cut(s)	269, 987
Asp718I	GGTACC	2 cut(s)	279, 601
AspS9I	GGNCC	5 cut(s)	176, 319, 409, 761, 1218
AsuC2I	CCSGG	1 cut(s)	261
AsuHPI	GGTGA	3 cut(s)	205, 442, 1015
AvaI	CYCGRG	1 cut(s)	743
AvaII	GGWCC	4 cut(s)	176, 409, 761, 1218
BaeGI	GKGCMC	2 cut(s)	339, 1270
BalI	TGGCCA	2 cut(s)	699, 1082
BanI	GGYRCC	4 cut(s)	256, 279, 601, 1265
BauI	CACGAG	1 cut(s)	284
BbsI	GAAGAC	2 cut(s)	643, 867
BbvI	GCAGC	5 cut(s)	419, 523, 568, 917, 920
BccI	CCATC	6 cut(s)	165, 504, 955, 1091, 1181, 1268
BcgI	CGANNNNNNTGC	2 cut(s)	172, 206
BciT130I	CCWGG	1 cut(s)	180
BcnI	CCSGG	1 cut(s)	261
BcoDI	GTCTC	3 cut(s)	523, 669, 1180
BfaI	CTAG	3 cut(s)	75, 429, 1253
BfmI	CTRYAG	1 cut(s)	616
BfuAI	ACCTGC	1 cut(s)	1229
BisI	GCNGC	6 cut(s)	433, 537, 540, 582, 906, 909
BlsI	GCNGC	6 cut(s)	434, 538, 541, 583, 907, 910
Bme1390I	CCNGG	2 cut(s)	180, 261
Bme18I	GGWCC	4 cut(s)	176, 409, 761, 1218
BmeT110I	CYCGRG	1 cut(s)	743
BmgT120I	GGNCC	5 cut(s)	176, 319, 409, 761, 1218
BmiI	GGNNCC	6 cut(s)	177, 258, 281, 603, 762, 1267
BmrFI	CCNGG	2 cut(s)	180, 261
BmrI	ACTGGG	1 cut(s)	1241
BmsI	GCATC	2 cut(s)	234, 1167
BmuI	ACTGGG	1 cut(s)	1241
BpiI	GAAGAC	2 cut(s)	643, 867
Bpu10I	CCTNAGC	1 cut(s)	140
BpuEI	CTTGAG	1 cut(s)	574
BpuMI	CCSGG	1 cut(s)	261
BsaJI	CCNNGG	5 cut(s)	120, 156, 260, 756, 1065
BsaWI	WCCGGW	1 cut(s)	61
BsaXI	ACNNNNNCTCC	4 cut(s)	497, 527, 768, 798
Bsc4I	CCNNNNNNNGG	5 cut(s)	90, 126, 289, 758, 1094
Bse1I	ACTGG	2 cut(s)	1220, 1236
Bse3DI	GCAATG	1 cut(s)	183
BseBI	CCWGG	1 cut(s)	180
BseDI	CCNNGG	5 cut(s)	120, 156, 260, 756, 1065
BseGI	GGATG	3 cut(s)	176, 867, 1083
BseLI	CCNNNNNNNGG	5 cut(s)	90, 126, 289, 758, 1094
BseMI	GCAATG	1 cut(s)	183
BseMII	CTCAG	1 cut(s)	111
BseNI	ACTGG	2 cut(s)	1220, 1236
BseSI	GKGCMC	2 cut(s)	339, 1270
BseXI	GCAGC	5 cut(s)	419, 523, 568, 917, 920
BseYI	CCCAGC	2 cut(s)	289, 316
Bsh1236I	CGCG	1 cut(s)	959
Bsh1285I	CGRYCG	1 cut(s)	277
BshFI	GGCC	5 cut(s)	161, 321, 699, 1082, 1101
BshNI	GGYRCC	4 cut(s)	256, 279, 601, 1265
BsiEI	CGRYCG	1 cut(s)	277
BsiHKCI	CYCGRG	1 cut(s)	743
BsiSI	CCGG	2 cut(s)	62, 260
BslFI	GGGAC	2 cut(s)	306, 774
BslI	CCNNNNNNNGG	5 cut(s)	90, 126, 289, 758, 1094
BsmAI	GTCTC	3 cut(s)	523, 669, 1180
BsmBI	CGTCTC	2 cut(s)	523, 1180
BsmFI	GGGAC	2 cut(s)	306, 774
BsmI	GAATGC	1 cut(s)	85
BsnI	GGCC	5 cut(s)	161, 321, 699, 1082, 1101
BsoBI	CYCGRG	1 cut(s)	743
Bsp1286I	GDGCHC	2 cut(s)	339, 1270
Bsp143I	GATC	4 cut(s)	207, 274, 717, 927
Bsp19I	CCATGG	3 cut(s)	120, 156, 1065
Bsp68I	TCGCGA	1 cut(s)	959
BspACI	CCGC	4 cut(s)	268, 540, 1013, 1042
BspANI	GGCC	5 cut(s)	161, 321, 699, 1082, 1101
BspCNI	CTCAG	1 cut(s)	110
BspFNI	CGCG	1 cut(s)	959
BspLI	GGNNCC	6 cut(s)	177, 258, 281, 603, 762, 1267
BspMI	ACCTGC	1 cut(s)	1229
BspPI	GGATC	3 cut(s)	215, 712, 922
BspT107I	GGYRCC	4 cut(s)	256, 279, 601, 1265
BsrDI	GCAATG	1 cut(s)	183
BsrI	ACTGG	2 cut(s)	1220, 1236
BssECI	CCNNGG	5 cut(s)	120, 156, 260, 756, 1065
BssMI	GATC	4 cut(s)	207, 274, 717, 927
BssSI	CACGAG	1 cut(s)	284
BssT1I	CCWWGG	4 cut(s)	120, 156, 756, 1065
Bst2BI	CACGAG	1 cut(s)	284
Bst2UI	CCWGG	1 cut(s)	180
Bst6I	CTCTTC	1 cut(s)	21
BstC8I	GCNNGC	4 cut(s)	300, 534, 697, 1170
BstDEI	CTNAG	2 cut(s)	97, 140
BstDSI	CCRYGG	3 cut(s)	120, 156, 1065
BstF5I	GGATG	3 cut(s)	176, 867, 1083
BstFNI	CGCG	1 cut(s)	959
BstKTI	GATC	4 cut(s)	210, 277, 720, 930
BstMAI	GTCTC	3 cut(s)	523, 669, 1180
BstMBI	GATC	4 cut(s)	207, 274, 717, 927
BstMCI	CGRYCG	1 cut(s)	277
BstMWI	GCNNNNNNNGC	7 cut(s)	29, 80, 265, 295, 343, 432, 578
BstNI	CCWGG	1 cut(s)	180
BstNSI	RCATGY	1 cut(s)	302
BstSCI	CCNGG	2 cut(s)	178, 259
BstSFI	CTRYAG	1 cut(s)	616
BstSLI	GKGCMC	2 cut(s)	339, 1270
BstUI	CGCG	1 cut(s)	959
BstV1I	GCAGC	5 cut(s)	419, 523, 568, 917, 920
BstV2I	GAAGAC	2 cut(s)	643, 867
BstX2I	RGATCY	1 cut(s)	717
BstXI	CCANNNNNNTGG	1 cut(s)	938
BstYI	RGATCY	1 cut(s)	717
BsuRI	GGCC	5 cut(s)	161, 321, 699, 1082, 1101
BtgI	CCRYGG	3 cut(s)	120, 156, 1065
BtgZI	GCGATG	1 cut(s)	974
BtsCI	GGATG	3 cut(s)	176, 867, 1083
BtsI	GCAGTG	1 cut(s)	114
BtsIMutI	CAGTG	1 cut(s)	114
BtuMI	TCGCGA	1 cut(s)	959
BveI	ACCTGC	1 cut(s)	1229
Cac8I	GCNNGC	4 cut(s)	300, 534, 697, 1170
Cfr13I	GGNCC	5 cut(s)	176, 319, 409, 761, 1218
Csp6I	GTAC	3 cut(s)	94, 280, 602
CviQI	GTAC	3 cut(s)	94, 280, 602
DdeI	CTNAG	2 cut(s)	97, 140
DpnI	GATC	4 cut(s)	209, 276, 719, 929
DpnII	GATC	4 cut(s)	207, 274, 717, 927
DrdI	GACNNNNNNGTC	1 cut(s)	1194
DseDI	GACNNNNNNGTC	1 cut(s)	1194
EaeI	YGGCCR	3 cut(s)	159, 697, 1080
Eam1104I	CTCTTC	1 cut(s)	21
EarI	CTCTTC	1 cut(s)	21
Eco130I	CCWWGG	4 cut(s)	120, 156, 756, 1065
Eco47I	GGWCC	4 cut(s)	176, 409, 761, 1218
Eco57I	CTGAAG	1 cut(s)	45
Eco88I	CYCGRG	1 cut(s)	743
EcoO109I	RGGNCCY	1 cut(s)	176
EcoRII	CCWGG	1 cut(s)	178
EcoT14I	CCWWGG	4 cut(s)	120, 156, 756, 1065
EcoT22I	ATGCAT	1 cut(s)	249
ErhI	CCWWGG	4 cut(s)	120, 156, 756, 1065
Esp3I	CGTCTC	2 cut(s)	523, 1180
FaqI	GGGAC	2 cut(s)	306, 774
FauI	CCCGC	2 cut(s)	1020, 1049
Fnu4HI	GCNGC	6 cut(s)	433, 537, 540, 582, 906, 909
FokI	GGATG	3 cut(s)	183, 854, 1070
Fsp4HI	GCNGC	6 cut(s)	433, 537, 540, 582, 906, 909
FspBI	CTAG	3 cut(s)	75, 429, 1253
GluI	GCNGC	6 cut(s)	433, 537, 540, 582, 906, 909
GsaI	CCCAGC	2 cut(s)	293, 320
HaeIII	GGCC	5 cut(s)	161, 321, 699, 1082, 1101
HapII	CCGG	2 cut(s)	62, 260
HinfI	GANTC	3 cut(s)	730, 1110, 1249
HpaII	CCGG	2 cut(s)	62, 260
HphI	GGTGA	3 cut(s)	205, 442, 1015
Hpy166II	GTNNAC	1 cut(s)	613
Hpy188I	TCNGA	2 cut(s)	644, 1109
Hpy188III	TCNNGA	2 cut(s)	958, 997
Hpy8I	GTNNAC	1 cut(s)	613
Hpy99I	CGWCG	1 cut(s)	16
HpyCH4IV	ACGT	2 cut(s)	609, 1189
HpyCH4V	TGCA	8 cut(s)	247, 435, 905, 1058, 1158, 1209, 1224, 1288
HpyF10VI	GCNNNNNNNGC	7 cut(s)	29, 80, 265, 295, 343, 432, 578
HpyF3I	CTNAG	2 cut(s)	97, 140
HpySE526I	ACGT	2 cut(s)	609, 1189
KpnI	GGTACC	2 cut(s)	283, 605
Kzo9I	GATC	4 cut(s)	207, 274, 717, 927
LmnI	GCTCC	3 cut(s)	578, 644, 1235
Lsp1109I	GCAGC	5 cut(s)	419, 523, 568, 917, 920
LweI	GCATC	2 cut(s)	234, 1167
MaeI	CTAG	3 cut(s)	75, 429, 1253
MaeII	ACGT	2 cut(s)	609, 1189
MaeIII	GTNAC	4 cut(s)	64, 112, 382, 557
MalI	GATC	4 cut(s)	209, 276, 719, 929
MboI	GATC	4 cut(s)	207, 274, 717, 927
MboII	GAAGA	5 cut(s)	38, 415, 643, 872, 1300
MflI	RGATCY	1 cut(s)	717
MhlI	GDGCHC	2 cut(s)	339, 1270
MlsI	TGGCCA	2 cut(s)	699, 1082
MluCI	AATT	3 cut(s)	348, 900, 1059
MluNI	TGGCCA	2 cut(s)	699, 1082
MmeI	TCCRAC	1 cut(s)	622
MnlI	CCTC	6 cut(s)	22, 27, 332, 383, 881, 1112
Mox20I	TGGCCA	2 cut(s)	699, 1082
Mph1103I	ATGCAT	1 cut(s)	249
MroXI	GAANNNNTTC	2 cut(s)	269, 987
MscI	TGGCCA	2 cut(s)	699, 1082
MseI	TTAA	2 cut(s)	509, 1315
MslI	CAYNNNNRTG	3 cut(s)	119, 797, 1076
Msp20I	TGGCCA	2 cut(s)	699, 1082
MspI	CCGG	2 cut(s)	62, 260
MspR9I	CCNGG	2 cut(s)	180, 261
Mva1269I	GAATGC	1 cut(s)	85
MvaI	CCWGG	1 cut(s)	180
MvnI	CGCG	1 cut(s)	959
MwoI	GCNNNNNNNGC	7 cut(s)	29, 80, 265, 295, 343, 432, 578
NciI	CCSGG	1 cut(s)	261
NcoI	CCATGG	3 cut(s)	120, 156, 1065
NdeII	GATC	4 cut(s)	207, 274, 717, 927
NlaIV	GGNNCC	6 cut(s)	177, 258, 281, 603, 762, 1267
NmeAIII	GCCGAG	1 cut(s)	187
NmuCI	GTSAC	3 cut(s)	64, 112, 382
NruI	TCGCGA	1 cut(s)	959
NsiI	ATGCAT	1 cut(s)	249
NspI	RCATGY	1 cut(s)	302
PaeI	GCATGC	1 cut(s)	302
PctI	GAATGC	1 cut(s)	85
PdmI	GAANNNNTTC	2 cut(s)	269, 987
PfeI	GAWTC	3 cut(s)	730, 1110, 1249
PflMI	CCANNNNNTGG	2 cut(s)	90, 289
PkrI	GCNGC	6 cut(s)	434, 538, 541, 583, 907, 910
Ple19I	CGATCG	1 cut(s)	277
PpuMI	RGGWCCY	1 cut(s)	176
Psp5II	RGGWCCY	1 cut(s)	176
Psp6I	CCWGG	1 cut(s)	178
PspFI	CCCAGC	2 cut(s)	289, 316
PspGI	CCWGG	1 cut(s)	178
PspN4I	GGNNCC	6 cut(s)	177, 258, 281, 603, 762, 1267
PspPI	GGNCC	5 cut(s)	176, 319, 409, 761, 1218
PspPPI	RGGWCCY	1 cut(s)	176
PsrI	GAACNNNNNNTAC	2 cut(s)	599, 631
PsuI	RGATCY	1 cut(s)	717
PvuI	CGATCG	1 cut(s)	277
RruI	TCGCGA	1 cut(s)	959
RsaI	GTAC	3 cut(s)	95, 281, 603
RsaNI	GTAC	3 cut(s)	94, 280, 602
RseI	CAYNNNNRTG	3 cut(s)	119, 797, 1076
SaqAI	TTAA	2 cut(s)	509, 1315
SatI	GCNGC	6 cut(s)	433, 537, 540, 582, 906, 909
Sau3AI	GATC	4 cut(s)	207, 274, 717, 927
Sau96I	GGNCC	5 cut(s)	176, 319, 409, 761, 1218
ScrFI	CCNGG	2 cut(s)	180, 261
SduI	GDGCHC	2 cut(s)	339, 1270
SfaNI	GCATC	2 cut(s)	234, 1167
SfcI	CTRYAG	1 cut(s)	616
SinI	GGWCC	4 cut(s)	176, 409, 761, 1218
SmiMI	CAYNNNNRTG	3 cut(s)	119, 797, 1076
SmlI	CTYRAG	1 cut(s)	553
SmoI	CTYRAG	1 cut(s)	553
SphI	GCATGC	1 cut(s)	302
Sse9I	AATT	3 cut(s)	348, 900, 1059
SsiI	CCGC	4 cut(s)	268, 540, 1013, 1042
SspI	AATATT	1 cut(s)	370
SspMI	CTAG	3 cut(s)	75, 429, 1253
StyD4I	CCNGG	2 cut(s)	178, 259
StyI	CCWWGG	4 cut(s)	120, 156, 756, 1065
TaiI	ACGT	2 cut(s)	612, 1192
TaqI	TCGA	4 cut(s)	14, 273, 882, 1292
TasI	AATT	3 cut(s)	348, 900, 1059
TauI	GCSGC	1 cut(s)	542
TfiI	GAWTC	3 cut(s)	730, 1110, 1249
Tru1I	TTAA	2 cut(s)	509, 1315
Tru9I	TTAA	2 cut(s)	509, 1315
TscAI	CASTG	1 cut(s)	121
TseFI	GTSAC	3 cut(s)	64, 112, 382
TseI	GCWGC	5 cut(s)	432, 536, 581, 905, 908
Tsp45I	GTSAC	3 cut(s)	64, 112, 382
TspDTI	ATGAA	3 cut(s)	17, 264, 1019
TspGWI	ACGGA	3 cut(s)	505, 861, 1288
TspRI	CASTG	1 cut(s)	121
Van91I	CCANNNNNTGG	2 cut(s)	90, 289
VpaK11BI	GGWCC	4 cut(s)	176, 409, 761, 1218
XceI	RCATGY	1 cut(s)	302
XmnI	GAANNNNTTC	2 cut(s)	269, 987
XspI	CTAG	3 cut(s)	75, 429, 1253
Zsp2I	ATGCAT	1 cut(s)	249

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.