Rroxscaffold_5G00372780

Lysine histidine transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0006982
Physical Location & Seq
Reverse (-)
54124264 .. 54126464
2201 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00372780.1

Sequence Viewer

Length: 753 bp
ATGCAGACGTGCAATCCGGTATCATTGATTTCTACCCTGTTTTCTTGGACTCTTGCTTGCAGTTACTCAACTATTGCATGGGCAGCTTCAATACACAAGGGGGTCGTACCAGATGTGGACTACAGCTACAGAGCCTCAACTACTCCTGGAACTGTGCTTAACTTCTTTGGTGGGTTGGGTGAGGTAGCATTTGCCTATGCTGGTCACAATGTGGTCCTGGAGATCCAAGCAACCATTCCTTCAACGCCGGAAAAGCCTTCCAAAGGTCCCATGTGGAAAGGAGTGCTTGTGGCATACATAGTTGTGGCTATATGCTACTTCCCTGTTGCTATTGTTGGGTTTTATGTCTTCGGAAATAGGGTTGATGACAACATACTCATCTCGCTGGAGAAACCAGCTTGGCTAATTGCAACTGCTAACATGTTTGTCGTAGTTCATGTGATTGGAAGCTATCAGATATATGCAATGGCGGTGTTTGACATGATTGAAACCTTTTTGGTTAAAAAAATGAACTTCTCACCCAGTTTCGTTCTCCGCTTTTTTACTCGAACTACATATGTTGCACCGTGGGGTGCAGTCGTCGGGAAGTCCCTTGACCTGACTTCTTCTCAAATGCGTGTGGACGAGGAGAGCACCAACCTCGTCACAAGATTATTTTCCATGCCTTTCGGAAAAGGATTTCTTGCCTTACGGGTAAGGGCTTGTGTTGTGATCTCTTGCGTCACCGAGTCGATACTCAATATTGTAGATTGA

Protein Analysis

250

Amino Acids

27.46

Weight (kDa)

7.61

Isoelectric Point (pI)

35.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 7 - 188 2.2e-36 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 470, 535
AclWI GGATC 1 cut(s) 217
AdeI CACNNNGTG 1 cut(s) 211
AfaI GTAC 1 cut(s) 108
AfiI CCNNNNNNNGG 1 cut(s) 263
AflIII ACRYGT 1 cut(s) 420
AgsI TTSAA 3 cut(s) 90, 243, 488
AjiI CACGTC 1 cut(s) 9
AjnI CCWGG 2 cut(s) 145, 216
AjuI GAANNNNNNNTTGG 2 cut(s) 219, 251
AluBI AGCT 4 cut(s) 86, 126, 398, 450
AluI AGCT 4 cut(s) 86, 126, 398, 450
Alw21I GWGCWC 1 cut(s) 635
AlwI GGATC 1 cut(s) 217
ApeKI GCWGC 1 cut(s) 83
AspS9I GGNCC 2 cut(s) 214, 266
AsuHPI GGTGA 3 cut(s) 191, 510, 715
AvaII GGWCC 2 cut(s) 214, 266
BbsI GAAGAC 1 cut(s) 340
Bbv12I GWGCWC 1 cut(s) 635
BbvI GCAGC 1 cut(s) 95
BcgI CGANNNNNNTGC 2 cut(s) 622, 656
BciT130I CCWGG 2 cut(s) 147, 218
BfmI CTRYAG 2 cut(s) 121, 127
BisI GCNGC 1 cut(s) 84
BlsI GCNGC 1 cut(s) 85
Bme1390I CCNGG 2 cut(s) 147, 218
Bme18I GGWCC 2 cut(s) 214, 266
BmgBI CACGTC 1 cut(s) 9
BmgT120I GGNCC 2 cut(s) 214, 266
BmiI GGNNCC 1 cut(s) 268
BmrFI CCNGG 2 cut(s) 147, 218
BmrI ACTGGG 1 cut(s) 516
BmuI ACTGGG 1 cut(s) 516
BpiI GAAGAC 1 cut(s) 340
BpmI CTGGAG 2 cut(s) 239, 407
BsaJI CCNNGG 1 cut(s) 566
BsaWI WCCGGW 1 cut(s) 16
BsaXI ACNNNNNCTCC 2 cut(s) 273, 303
Bsc4I CCNNNNNNNGG 1 cut(s) 263
Bse1I ACTGG 1 cut(s) 522
Bse3DI GCAATG 1 cut(s) 471
BseBI CCWGG 2 cut(s) 147, 218
BseDI CCNNGG 1 cut(s) 566
BseLI CCNNNNNNNGG 1 cut(s) 263
BseMI GCAATG 1 cut(s) 471
BseNI ACTGG 1 cut(s) 522
BseRI GAGGAG 1 cut(s) 641
BseXI GCAGC 1 cut(s) 95
BsgI GTGCAG 1 cut(s) 594
BsiHKAI GWGCWC 1 cut(s) 635
BsiSI CCGG 2 cut(s) 17, 248
BslFI GGGAC 2 cut(s) 252, 574
BslI CCNNNNNNNGG 1 cut(s) 263
BsmFI GGGAC 2 cut(s) 252, 574
Bsp1286I GDGCHC 1 cut(s) 635
Bsp143I GATC 2 cut(s) 222, 711
BspACI CCGC 2 cut(s) 470, 535
BspLI GGNNCC 1 cut(s) 268
BspPI GGATC 1 cut(s) 217
BsrDI GCAATG 1 cut(s) 471
BsrI ACTGG 1 cut(s) 522
BssECI CCNNGG 1 cut(s) 566
BssMI GATC 2 cut(s) 222, 711
Bst2UI CCWGG 2 cut(s) 147, 218
Bst4CI ACNGT 2 cut(s) 154, 567
BstC8I GCNNGC 1 cut(s) 58
BstDSI CCRYGG 1 cut(s) 566
BstENI CCTNNNNNAGG 1 cut(s) 261
BstKTI GATC 2 cut(s) 225, 714
BstMBI GATC 2 cut(s) 222, 711
BstMWI GCNNNNNNNGC 2 cut(s) 83, 253
BstNI CCWGG 2 cut(s) 147, 218
BstNSI RCATGY 1 cut(s) 424
BstSCI CCNGG 2 cut(s) 145, 216
BstSFI CTRYAG 2 cut(s) 121, 127
BstV1I GCAGC 1 cut(s) 95
BstV2I GAAGAC 1 cut(s) 340
BstX2I RGATCY 1 cut(s) 222
BstYI RGATCY 1 cut(s) 222
BtgI CCRYGG 1 cut(s) 566
BtrI CACGTC 1 cut(s) 9
Cac8I GCNNGC 1 cut(s) 58
Cfr13I GGNCC 2 cut(s) 214, 266
CseI GACGC 1 cut(s) 709
Csp6I GTAC 1 cut(s) 107
CviAII CATG 6 cut(s) 78, 271, 421, 437, 481, 661
CviJI RGCY 9 cut(s) 86, 126, 134, 256, 308, 398, 403, 450, 701
CviKI_1 RGCY 9 cut(s) 86, 126, 134, 256, 308, 398, 403, 450, 701
CviQI GTAC 1 cut(s) 107
DpnI GATC 2 cut(s) 224, 713
DpnII GATC 2 cut(s) 222, 711
DraIII CACNNNGTG 1 cut(s) 211
Eco47I GGWCC 2 cut(s) 214, 266
EcoNI CCTNNNNNAGG 1 cut(s) 261
EcoO109I RGGNCCY 1 cut(s) 266
EcoRII CCWGG 2 cut(s) 145, 216
FaeI CATG 6 cut(s) 81, 274, 424, 440, 484, 664
FalI AAGNNNNNCTT 4 cut(s) 270, 302, 666, 698
FaqI GGGAC 2 cut(s) 252, 574
FatI CATG 6 cut(s) 77, 270, 420, 436, 480, 660
FauNDI CATATG 1 cut(s) 556
Fnu4HI GCNGC 1 cut(s) 84
Fsp4HI GCNGC 1 cut(s) 84
GluI GCNGC 1 cut(s) 84
GsuI CTGGAG 2 cut(s) 239, 407
HapII CCGG 2 cut(s) 17, 248
HgaI GACGC 1 cut(s) 709
Hin1II CATG 6 cut(s) 81, 274, 424, 440, 484, 664
HinfI GANTC 2 cut(s) 49, 728
HpaII CCGG 2 cut(s) 17, 248
HphI GGTGA 3 cut(s) 191, 510, 715
Hpy166II GTNNAC 2 cut(s) 118, 622
Hpy188I TCNGA 3 cut(s) 353, 456, 671
Hpy188III TCNNGA 1 cut(s) 583
Hpy8I GTNNAC 2 cut(s) 118, 622
Hpy99I CGWCG 1 cut(s) 584
HpyAV CCTTC 2 cut(s) 249, 267
HpyCH4III ACNGT 2 cut(s) 154, 567
HpyCH4IV ACGT 1 cut(s) 8
HpyCH4V TGCA 8 cut(s) 4, 12, 60, 77, 410, 464, 563, 575
HpyF10VI GCNNNNNNNGC 2 cut(s) 83, 253
HpySE526I ACGT 1 cut(s) 8
Hsp92II CATG 6 cut(s) 81, 274, 424, 440, 484, 664
Kzo9I GATC 2 cut(s) 222, 711
Lsp1109I GCAGC 1 cut(s) 95
MaeII ACGT 1 cut(s) 8
MaeIII GTNAC 4 cut(s) 62, 203, 643, 721
MalI GATC 2 cut(s) 224, 713
MboI GATC 2 cut(s) 222, 711
MboII GAAGA 2 cut(s) 340, 597
MflI RGATCY 1 cut(s) 222
MhlI GDGCHC 1 cut(s) 635
MluCI AATT 1 cut(s) 405
MlyI GAGTC 2 cut(s) 43, 737
MnlI CCTC 4 cut(s) 145, 175, 619, 650
MseI TTAA 2 cut(s) 159, 501
MslI CAYNNNNRTG 1 cut(s) 302
MspI CCGG 2 cut(s) 17, 248
MspR9I CCNGG 2 cut(s) 147, 218
MvaI CCWGG 2 cut(s) 147, 218
MwoI GCNNNNNNNGC 2 cut(s) 83, 253
NdeI CATATG 1 cut(s) 556
NdeII GATC 2 cut(s) 222, 711
NlaIII CATG 6 cut(s) 81, 274, 424, 440, 484, 664
NlaIV GGNNCC 1 cut(s) 268
NmuCI GTSAC 3 cut(s) 203, 643, 721
NspI RCATGY 1 cut(s) 424
PciI ACATGT 1 cut(s) 420
PfoI TCCNGGA 2 cut(s) 145, 216
PkrI GCNGC 1 cut(s) 85
PleI GAGTC 2 cut(s) 43, 736
PpsI GAGTC 2 cut(s) 43, 736
PpuMI RGGWCCY 1 cut(s) 266
PscI ACATGT 1 cut(s) 420
Psp5II RGGWCCY 1 cut(s) 266
Psp6I CCWGG 2 cut(s) 145, 216
PspGI CCWGG 2 cut(s) 145, 216
PspN4I GGNNCC 1 cut(s) 268
PspPI GGNCC 2 cut(s) 214, 266
PspPPI RGGWCCY 1 cut(s) 266
PsuI RGATCY 1 cut(s) 222
RsaI GTAC 1 cut(s) 108
RsaNI GTAC 1 cut(s) 107
RseI CAYNNNNRTG 1 cut(s) 302
SaqAI TTAA 2 cut(s) 159, 501
SatI GCNGC 1 cut(s) 84
Sau3AI GATC 2 cut(s) 222, 711
Sau96I GGNCC 2 cut(s) 214, 266
SchI GAGTC 2 cut(s) 43, 737
ScrFI CCNGG 2 cut(s) 147, 218
SduI GDGCHC 1 cut(s) 635
SfcI CTRYAG 2 cut(s) 121, 127
SinI GGWCC 2 cut(s) 214, 266
SmiMI CAYNNNNRTG 1 cut(s) 302
Sse9I AATT 1 cut(s) 405
SsiI CCGC 2 cut(s) 470, 535
SspI AATATT 1 cut(s) 742
StyD4I CCNGG 2 cut(s) 145, 216
TaaI ACNGT 2 cut(s) 154, 567
TaiI ACGT 1 cut(s) 11
TaqI TCGA 2 cut(s) 547, 731
TasI AATT 1 cut(s) 405
Tru1I TTAA 2 cut(s) 159, 501
Tru9I TTAA 2 cut(s) 159, 501
TseFI GTSAC 3 cut(s) 203, 643, 721
TseI GCWGC 1 cut(s) 83
Tsp45I GTSAC 3 cut(s) 203, 643, 721
TspDTI ATGAA 2 cut(s) 425, 524
VpaK11BI GGWCC 2 cut(s) 214, 266
XagI CCTNNNNNAGG 1 cut(s) 261
XceI RCATGY 1 cut(s) 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.