Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0006982

Physical Location & Seq

Genomic Coordinates Forward (+)

6319436 .. 6322158

Gene Structure 2723 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom16g09430.4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1326 bp

ATGTTTGAAGGGCGTCAGAGGAAGCTGAAGAGGCGAGGCAGAAGCAGAAGCAGATTGACGATTGGCTTCCGGTCACTTCTGCTAGGAATGCCAAATGGTGGTACTCAGCTTTCCACAATGTCACTGCCATGGTTGGGGCTGGTGTCCTCAGCCTTCCCTATGCCATGGCTGAGCTTGGATGGGGTCCTGGTATTGCTGTCATGCTTCTGTCATGGATCGATCACCCTATACACACTATGGCAAATGGTTGAGATGCATGAAATGGTGCCGGGGAAGCGGTTCGATCGGTACCACGAGCTGGGACAGCATGCCTTTGGGGAAAAGCTGGGGCTCTGGGTCGTTGTGCCCCAACAGCTAATTGTGGAAGTTGGTGTCAATATTGTCTATATGGTCACAGGAGGAAAATCATTGAAGAAGGTCCATGATGTGATTTGCCCTAGCTGCAAAGACATCAAACTCACCTTATGGATTTTGATTTTTGCTTCTGCCCACTTTGTTCTCTCCCATCTCCCCAACTTAAACTCCGTCTCTGGTATCTCGCTTGCTGCCGCAGTCATGTCCTTGAGTTACTCAACGATTGCGTGGGGAGCTGCTATTCACAAGGGGAAGGTACCGAACGTGGACTACAGCTACAAAGCTAAATCCAATGTCGGAGCTGCCTTCAACTTCTTTAGTGGGTTGGGAGACATAGCATTTGCCTACGCTGGCCACAATGTCGTTTTGGAGATCCAAGCAACGATTCCTTCCACACCCGAGAAGCCTTCCAAGGGACCAATGTGGAAAGGAGTTATTGTAGCATACATTGTGGTGGCTTTGTGCTACTTCCCTGTTTGTTTTGTTGGCTACTATATTTACGGAAACTCTGTTGAAGACAACATCCTCATCTCACTCGAAAAACCTGTCTGGCTAATTGCAGCAGCTAACATGTTTGTTGTGATCCATGTCATTGGAAGCTACCAAGTTTTCGCAATGGGAGTTTTTGACATGGTTGAAACTTTTCTGGTCAAGAAAATGAACTTCCCACCTTCTTTCACCCTTCGCTTTATTACTCGCACGATATATGTTGCAATTACCATGGTCATTGGTATGGCCATCCCTTTCTTTGGTGGCCTTCTCGGATTCTTTGGAGGATTTGCTTTCGCCCCAACCACATACTTCCTTCCCTGCATCATGTGGCTCGCCATATACAAACCGAGACGTTTCAGTCTATCTTGGTGCATAAACTGGACCTGCATTATACTGGGAGTTACCTTGATGATTCTAGCACCCATCGGTGCCCTACGGAGTATCATCTTGCAATCGAAAACCTACACATTCTTCTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003333 GO:0003674 GO:0005215 GO:0005342 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006865 GO:0008150 GO:0008509 GO:0008514 GO:0015075 GO:0015171 GO:0015318 GO:0015711 GO:0015849 GO:0016020 GO:0022857 GO:0034220 GO:0044464 GO:0046942 GO:0046943 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0071944 GO:0098656 GO:1903825 GO:1905039

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Aa_trans

Protein Analysis

442

Amino Acids

49.0

Weight (kDa)

9.5

Isoelectric Point (pI)

42.35

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006982)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G24400 AT1G67640
fragaria_vesca	FvH4_4g24381 FvH4_4g24381 FvH4_4g24390
malus_domestica	MD13G1111700.v1.1 MD16G1111300.v1.1
prunus_persica	Prupe.1G233800_v2.0.a1
pyrus_communis	pycom16g09430
rosa_chinensis	RchiOBHm_Chr4g0431231 RchiOBHm_Chr4g0431241
rosa_laevigata	RLG00000006916 RLG00000006917
rosa_multiflora	Rmu_sc0005258.1_g000008 Rmu_sc0039160.1_g000001
rosa_roxburghii	Rroxscaffold_5G00372770 Rroxscaffold_5G00372780
rosa_rugosa	Rorug04G0249400 Rorug04G0249500 Rorug04G0249600
rosa_samantha	Rh4AG305500 Rh4BG312800 Rh4BG312900
rosa_wichuraiana	Rw4G026550 Rw4G026560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1203
Acc36I	ACCTGC	1 cut(s)	1238
Acc65I	GGTACC	2 cut(s)	288, 610
AccB1I	GGYRCC	4 cut(s)	265, 288, 610, 1274
AccB7I	CCANNNNNTGG	2 cut(s)	98, 298
AciI	CCGC	2 cut(s)	277, 549
AclWI	GGATC	3 cut(s)	223, 721, 931
AcoI	YGGCCR	2 cut(s)	706, 1089
AcuI	CTGAAG	1 cut(s)	47
AcyI	GRCGYC	1 cut(s)	13
AfaI	GTAC	3 cut(s)	103, 290, 612
AfiI	CCNNNNNNNGG	5 cut(s)	98, 134, 298, 767, 1103
AflIII	ACRYGT	1 cut(s)	924
AgsI	TTSAA	5 cut(s)	8, 412, 664, 869, 992
AjnI	CCWGG	1 cut(s)	186
AjuI	GAANNNNNNNTTGG	2 cut(s)	723, 755
Alw26I	GTCTC	3 cut(s)	532, 678, 1189
AlwI	GGATC	3 cut(s)	223, 721, 931
Ama87I	CYCGRG	1 cut(s)	752
AoxI	GGCC	3 cut(s)	706, 1089, 1108
ApeKI	GCWGC	6 cut(s)	441, 545, 590, 656, 914, 917
ArsI	GACNNNNNNTTYG	4 cut(s)	677, 709, 885, 917
Asp700I	GAANNNNTTC	2 cut(s)	278, 996
Asp718I	GGTACC	2 cut(s)	288, 610
AspS9I	GGNCC	4 cut(s)	184, 418, 770, 1227
AsuC2I	CCSGG	1 cut(s)	270
AsuHPI	GGTGA	3 cut(s)	214, 451, 1024
AvaI	CYCGRG	1 cut(s)	752
AvaII	GGWCC	4 cut(s)	184, 418, 770, 1227
BaeGI	GKGCMC	2 cut(s)	348, 1279
BalI	TGGCCA	2 cut(s)	708, 1091
BanI	GGYRCC	4 cut(s)	265, 288, 610, 1274
BanII	GRGCYC	1 cut(s)	333
BauI	CACGAG	1 cut(s)	293
BbsI	GAAGAC	1 cut(s)	876
BbvCI	CCTCAGC	1 cut(s)	148
BbvI	GCAGC	6 cut(s)	428, 532, 577, 643, 926, 929
BccI	CCATC	4 cut(s)	173, 513, 1100, 1277
BciT130I	CCWGG	1 cut(s)	188
BcnI	CCSGG	1 cut(s)	270
BcoDI	GTCTC	3 cut(s)	532, 678, 1189
BfaI	CTAG	3 cut(s)	83, 438, 1262
BfmI	CTRYAG	1 cut(s)	625
BfuAI	ACCTGC	1 cut(s)	1238
BisI	GCNGC	7 cut(s)	442, 546, 549, 591, 657, 915, 918
BlpI	GCTNAGC	1 cut(s)	170
BlsI	GCNGC	7 cut(s)	443, 547, 550, 592, 658, 916, 919
Bme1390I	CCNGG	2 cut(s)	188, 270
Bme18I	GGWCC	4 cut(s)	184, 418, 770, 1227
BmeT110I	CYCGRG	1 cut(s)	752
BmgT120I	GGNCC	4 cut(s)	184, 418, 770, 1227
BmiI	GGNNCC	6 cut(s)	185, 267, 290, 612, 771, 1276
BmrFI	CCNGG	2 cut(s)	188, 270
BmrI	ACTGGG	1 cut(s)	1250
BmsI	GCATC	2 cut(s)	243, 1176
BmuI	ACTGGG	1 cut(s)	1250
BpiI	GAAGAC	1 cut(s)	876
Bpu10I	CCTNAGC	1 cut(s)	148
Bpu1102I	GCTNAGC	1 cut(s)	170
BpuEI	CTTGAG	1 cut(s)	583
BpuMI	CCSGG	1 cut(s)	270
Bsa29I	ATCGAT	1 cut(s)	218
BsaHI	GRCGYC	1 cut(s)	13
BsaJI	CCNNGG	5 cut(s)	128, 164, 269, 765, 1074
BsaWI	WCCGGW	1 cut(s)	69
BsaXI	ACNNNNNCTCC	4 cut(s)	506, 536, 777, 807
Bsc4I	CCNNNNNNNGG	5 cut(s)	98, 134, 298, 767, 1103
Bse1I	ACTGG	2 cut(s)	1229, 1245
Bse3DI	GCAATG	1 cut(s)	975
BseBI	CCWGG	1 cut(s)	188
BseCI	ATCGAT	1 cut(s)	218
BseDI	CCNNGG	5 cut(s)	128, 164, 269, 765, 1074
BseGI	GGATG	3 cut(s)	184, 876, 1092
BseLI	CCNNNNNNNGG	5 cut(s)	98, 134, 298, 767, 1103
BseMI	GCAATG	1 cut(s)	975
BseMII	CTCAG	3 cut(s)	119, 161, 162
BseNI	ACTGG	2 cut(s)	1229, 1245
BseSI	GKGCMC	2 cut(s)	348, 1279
BseXI	GCAGC	6 cut(s)	428, 532, 577, 643, 926, 929
BseYI	CCCAGC	2 cut(s)	298, 325
Bsh1285I	CGRYCG	1 cut(s)	286
BshFI	GGCC	3 cut(s)	708, 1091, 1110
BshNI	GGYRCC	4 cut(s)	265, 288, 610, 1274
BshVI	ATCGAT	1 cut(s)	218
BsiEI	CGRYCG	1 cut(s)	286
BsiHKCI	CYCGRG	1 cut(s)	752
BsiSI	CCGG	2 cut(s)	70, 269
BslFI	GGGAC	2 cut(s)	315, 783
BslI	CCNNNNNNNGG	5 cut(s)	98, 134, 298, 767, 1103
BsmAI	GTCTC	3 cut(s)	532, 678, 1189
BsmBI	CGTCTC	2 cut(s)	532, 1189
BsmFI	GGGAC	2 cut(s)	315, 783
BsmI	GAATGC	1 cut(s)	93
BsnI	GGCC	3 cut(s)	708, 1091, 1110
BsoBI	CYCGRG	1 cut(s)	752
Bsp1286I	GDGCHC	3 cut(s)	333, 348, 1279
Bsp143I	GATC	5 cut(s)	215, 219, 283, 726, 936
Bsp1720I	GCTNAGC	1 cut(s)	170
Bsp19I	CCATGG	3 cut(s)	128, 164, 1074
BspACI	CCGC	2 cut(s)	277, 549
BspANI	GGCC	3 cut(s)	708, 1091, 1110
BspCNI	CTCAG	3 cut(s)	118, 161, 162
BspDI	ATCGAT	1 cut(s)	218
BspLI	GGNNCC	6 cut(s)	185, 267, 290, 612, 771, 1276
BspMI	ACCTGC	1 cut(s)	1238
BspPI	GGATC	3 cut(s)	223, 721, 931
BspT107I	GGYRCC	4 cut(s)	265, 288, 610, 1274
BsrDI	GCAATG	1 cut(s)	975
BsrI	ACTGG	2 cut(s)	1229, 1245
BssECI	CCNNGG	5 cut(s)	128, 164, 269, 765, 1074
BssMI	GATC	5 cut(s)	215, 219, 283, 726, 936
BssNI	GRCGYC	1 cut(s)	13
BssSI	CACGAG	1 cut(s)	293
BssT1I	CCWWGG	4 cut(s)	128, 164, 765, 1074
Bst2BI	CACGAG	1 cut(s)	293
Bst2UI	CCWGG	1 cut(s)	188
Bst6I	CTCTTC	1 cut(s)	23
BstACI	GRCGYC	1 cut(s)	13
BstC8I	GCNNGC	4 cut(s)	309, 543, 706, 1179
BstDEI	CTNAG	3 cut(s)	105, 148, 170
BstDSI	CCRYGG	3 cut(s)	128, 164, 1074
BstF5I	GGATG	3 cut(s)	184, 876, 1092
BstKTI	GATC	5 cut(s)	218, 222, 286, 729, 939
BstMAI	GTCTC	3 cut(s)	532, 678, 1189
BstMBI	GATC	5 cut(s)	215, 219, 283, 726, 936
BstMCI	CGRYCG	1 cut(s)	286
BstMWI	GCNNNNNNNGC	7 cut(s)	31, 88, 274, 304, 352, 441, 587
BstNI	CCWGG	1 cut(s)	188
BstNSI	RCATGY	2 cut(s)	311, 928
BstSCI	CCNGG	2 cut(s)	186, 268
BstSFI	CTRYAG	1 cut(s)	625
BstSLI	GKGCMC	2 cut(s)	348, 1279
BstV1I	GCAGC	6 cut(s)	428, 532, 577, 643, 926, 929
BstV2I	GAAGAC	1 cut(s)	876
BstX2I	RGATCY	1 cut(s)	726
BstXI	CCANNNNNNTGG	1 cut(s)	947
BstYI	RGATCY	1 cut(s)	726
Bsu15I	ATCGAT	1 cut(s)	218
BsuRI	GGCC	3 cut(s)	708, 1091, 1110
BsuTUI	ATCGAT	1 cut(s)	218
BtgI	CCRYGG	3 cut(s)	128, 164, 1074
BtsCI	GGATG	3 cut(s)	184, 876, 1092
BtsI	GCAGTG	1 cut(s)	122
BtsIMutI	CAGTG	1 cut(s)	122
BveI	ACCTGC	1 cut(s)	1238
Cac8I	GCNNGC	4 cut(s)	309, 543, 706, 1179
Cfr13I	GGNCC	4 cut(s)	184, 418, 770, 1227
ClaI	ATCGAT	1 cut(s)	218
CseI	GACGC	1 cut(s)	2
Csp6I	GTAC	3 cut(s)	102, 289, 611
CviQI	GTAC	3 cut(s)	102, 289, 611
DdeI	CTNAG	3 cut(s)	105, 148, 170
DpnI	GATC	5 cut(s)	217, 221, 285, 728, 938
DpnII	GATC	5 cut(s)	215, 219, 283, 726, 936
DrdI	GACNNNNNNGTC	1 cut(s)	1203
DseDI	GACNNNNNNGTC	1 cut(s)	1203
EaeI	YGGCCR	2 cut(s)	706, 1089
Eam1104I	CTCTTC	1 cut(s)	23
EarI	CTCTTC	1 cut(s)	23
Eco130I	CCWWGG	4 cut(s)	128, 164, 765, 1074
Eco24I	GRGCYC	1 cut(s)	333
Eco47I	GGWCC	4 cut(s)	184, 418, 770, 1227
Eco57I	CTGAAG	1 cut(s)	47
Eco88I	CYCGRG	1 cut(s)	752
EcoO109I	RGGNCCY	1 cut(s)	184
EcoRII	CCWGG	1 cut(s)	186
EcoT14I	CCWWGG	4 cut(s)	128, 164, 765, 1074
EcoT22I	ATGCAT	1 cut(s)	258
EcoT38I	GRGCYC	1 cut(s)	333
ErhI	CCWWGG	4 cut(s)	128, 164, 765, 1074
Esp3I	CGTCTC	2 cut(s)	532, 1189
FaqI	GGGAC	2 cut(s)	315, 783
Fnu4HI	GCNGC	7 cut(s)	442, 546, 549, 591, 657, 915, 918
FokI	GGATG	3 cut(s)	191, 863, 1079
FriOI	GRGCYC	1 cut(s)	333
Fsp4HI	GCNGC	7 cut(s)	442, 546, 549, 591, 657, 915, 918
FspBI	CTAG	3 cut(s)	83, 438, 1262
GluI	GCNGC	7 cut(s)	442, 546, 549, 591, 657, 915, 918
GsaI	CCCAGC	2 cut(s)	302, 329
HaeIII	GGCC	3 cut(s)	708, 1091, 1110
HapII	CCGG	2 cut(s)	70, 269
HgaI	GACGC	1 cut(s)	2
Hin1I	GRCGYC	1 cut(s)	13
HinfI	GANTC	3 cut(s)	739, 1119, 1258
HpaII	CCGG	2 cut(s)	70, 269
HphI	GGTGA	3 cut(s)	214, 451, 1024
Hpy166II	GTNNAC	1 cut(s)	622
Hpy188I	TCNGA	3 cut(s)	18, 653, 1118
Hpy188III	TCNNGA	1 cut(s)	1006
Hpy8I	GTNNAC	1 cut(s)	622
HpyCH4IV	ACGT	2 cut(s)	618, 1198
HpyCH4V	TGCA	8 cut(s)	256, 444, 914, 1067, 1167, 1218, 1233, 1297
HpyF10VI	GCNNNNNNNGC	7 cut(s)	31, 88, 274, 304, 352, 441, 587
HpyF3I	CTNAG	3 cut(s)	105, 148, 170
HpySE526I	ACGT	2 cut(s)	618, 1198
Hsp92I	GRCGYC	1 cut(s)	13
KpnI	GGTACC	2 cut(s)	292, 614
Kzo9I	GATC	5 cut(s)	215, 219, 283, 726, 936
LmnI	GCTCC	2 cut(s)	587, 653
Lsp1109I	GCAGC	6 cut(s)	428, 532, 577, 643, 926, 929
LweI	GCATC	2 cut(s)	243, 1176
MaeI	CTAG	3 cut(s)	83, 438, 1262
MaeII	ACGT	2 cut(s)	618, 1198
MaeIII	GTNAC	5 cut(s)	72, 120, 391, 566, 1246
MalI	GATC	5 cut(s)	217, 221, 285, 728, 938
MboI	GATC	5 cut(s)	215, 219, 283, 726, 936
MboII	GAAGA	4 cut(s)	40, 424, 881, 1309
MflI	RGATCY	1 cut(s)	726
MhlI	GDGCHC	3 cut(s)	333, 348, 1279
MlsI	TGGCCA	2 cut(s)	708, 1091
MluCI	AATT	3 cut(s)	357, 909, 1068
MluNI	TGGCCA	2 cut(s)	708, 1091
MmeI	TCCRAC	1 cut(s)	631
MnlI	CCTC	7 cut(s)	12, 24, 29, 157, 392, 890, 1121
Mox20I	TGGCCA	2 cut(s)	708, 1091
Mph1103I	ATGCAT	1 cut(s)	258
MroXI	GAANNNNTTC	2 cut(s)	278, 996
MscI	TGGCCA	2 cut(s)	708, 1091
MseI	TTAA	2 cut(s)	518, 1324
MslI	CAYNNNNRTG	3 cut(s)	127, 806, 1085
Msp20I	TGGCCA	2 cut(s)	708, 1091
MspI	CCGG	2 cut(s)	70, 269
MspR9I	CCNGG	2 cut(s)	188, 270
Mva1269I	GAATGC	1 cut(s)	93
MvaI	CCWGG	1 cut(s)	188
MwoI	GCNNNNNNNGC	7 cut(s)	31, 88, 274, 304, 352, 441, 587
NciI	CCSGG	1 cut(s)	270
NcoI	CCATGG	3 cut(s)	128, 164, 1074
NdeII	GATC	5 cut(s)	215, 219, 283, 726, 936
NlaIV	GGNNCC	6 cut(s)	185, 267, 290, 612, 771, 1276
NmuCI	GTSAC	3 cut(s)	72, 120, 391
NsiI	ATGCAT	1 cut(s)	258
NspI	RCATGY	2 cut(s)	311, 928
PaeI	GCATGC	1 cut(s)	311
PciI	ACATGT	1 cut(s)	924
PctI	GAATGC	1 cut(s)	93
PdmI	GAANNNNTTC	2 cut(s)	278, 996
PfeI	GAWTC	3 cut(s)	739, 1119, 1258
PflMI	CCANNNNNTGG	2 cut(s)	98, 298
PkrI	GCNGC	7 cut(s)	443, 547, 550, 592, 658, 916, 919
Ple19I	CGATCG	1 cut(s)	286
PpuMI	RGGWCCY	1 cut(s)	184
PscI	ACATGT	1 cut(s)	924
Psp5II	RGGWCCY	1 cut(s)	184
Psp6I	CCWGG	1 cut(s)	186
PspFI	CCCAGC	2 cut(s)	298, 325
PspGI	CCWGG	1 cut(s)	186
PspN4I	GGNNCC	6 cut(s)	185, 267, 290, 612, 771, 1276
PspPI	GGNCC	4 cut(s)	184, 418, 770, 1227
PspPPI	RGGWCCY	1 cut(s)	184
PsrI	GAACNNNNNNTAC	2 cut(s)	608, 640
PsuI	RGATCY	1 cut(s)	726
PvuI	CGATCG	1 cut(s)	286
RsaI	GTAC	3 cut(s)	103, 290, 612
RsaNI	GTAC	3 cut(s)	102, 289, 611
RseI	CAYNNNNRTG	3 cut(s)	127, 806, 1085
SaqAI	TTAA	2 cut(s)	518, 1324
SatI	GCNGC	7 cut(s)	442, 546, 549, 591, 657, 915, 918
Sau3AI	GATC	5 cut(s)	215, 219, 283, 726, 936
Sau96I	GGNCC	4 cut(s)	184, 418, 770, 1227
ScrFI	CCNGG	2 cut(s)	188, 270
SduI	GDGCHC	3 cut(s)	333, 348, 1279
SfaNI	GCATC	2 cut(s)	243, 1176
SfcI	CTRYAG	1 cut(s)	625
SinI	GGWCC	4 cut(s)	184, 418, 770, 1227
SmiMI	CAYNNNNRTG	3 cut(s)	127, 806, 1085
SmlI	CTYRAG	1 cut(s)	562
SmoI	CTYRAG	1 cut(s)	562
SphI	GCATGC	1 cut(s)	311
Sse9I	AATT	3 cut(s)	357, 909, 1068
SsiI	CCGC	2 cut(s)	277, 549
SspI	AATATT	1 cut(s)	379
SspMI	CTAG	3 cut(s)	83, 438, 1262
StyD4I	CCNGG	2 cut(s)	186, 268
StyI	CCWWGG	4 cut(s)	128, 164, 765, 1074
TaiI	ACGT	2 cut(s)	621, 1201
TaqI	TCGA	4 cut(s)	218, 282, 891, 1301
TasI	AATT	3 cut(s)	357, 909, 1068
TauI	GCSGC	1 cut(s)	551
TfiI	GAWTC	3 cut(s)	739, 1119, 1258
Tru1I	TTAA	2 cut(s)	518, 1324
Tru9I	TTAA	2 cut(s)	518, 1324
TscAI	CASTG	1 cut(s)	129
TseFI	GTSAC	3 cut(s)	72, 120, 391
TseI	GCWGC	6 cut(s)	441, 545, 590, 656, 914, 917
Tsp45I	GTSAC	3 cut(s)	72, 120, 391
TspDTI	ATGAA	2 cut(s)	273, 1028
TspGWI	ACGGA	3 cut(s)	514, 870, 1297
TspRI	CASTG	1 cut(s)	129
Van91I	CCANNNNNTGG	2 cut(s)	98, 298
VpaK11BI	GGWCC	4 cut(s)	184, 418, 770, 1227
XceI	RCATGY	2 cut(s)	311, 928
XmnI	GAANNNNTTC	2 cut(s)	278, 996
XspI	CTAG	3 cut(s)	83, 438, 1262
Zsp2I	ATGCAT	1 cut(s)	258

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.