Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0003218

Physical Location & Seq

Genomic Coordinates Forward (+)

10427111 .. 10432645

Gene Structure 5535 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1136100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1341 bp

ATGGTGATCGTGAGCAACAGTCGAAATTACAGTAACAAGGGAATGCGACTGGGGAAATACGAGCTCGGAAGGACACTTGGCGAGGGTAATTTCGGGAAAGTCAAATTTGCTAAGAACGTCGAGTCCGGCCAGTCCTTTGCCGTTAAGATTCTGGAGAAGACCAAAATTATTGACCTTAAATTCACCGACCAGATAAAGAATGAGATTGGAACATTGAAACTACTGAAGCATCCAAACGTTGTCAGATTACATGAGGTTGTAGCAAGCAAAACCAAGATTTACATGGTCCTCGAATATGTTACTGGCGGCGAATTGTTTGACAAAATTGCACACAACGGAAAACTTACAGAGACCGAAGGCAGGAAGCTTTTCCAACAATTAATCGACGGTGTGAGCTACTGTCACAACAAAGGCGTCTCCCATCGGGATCTTAAGCTGGAGAATGTTCTTGTCGATGCTAAGGGGAACATAAAGATATCGGACTTTGGCCTTAGCGCTTTGCCCCAGGATTTTCGGGAAGATGGTTTGCTTCATACAACCTGTGGAAGCCCCAACTATGTTGCTCCCGAGATTCTTGCTAACAAAGGTTATGATGGTGCCACCTCCGATGTATGGTCATGTGGTGTCATCTTATACGTCATTCTCACCGGATTTCTCCCTTTCGATGATAGAAATCTTGCAGTTCTCTATCAGAAGATCTTAAAGGGCGAAACTTCGATACCGAAATGGTTATCACCCGGTGCAAAAAACTTGCTCAAGAGGATTCTCGATCCCAACCCTGCTACCAGAATAACAATGACTGGCATCAAGCCAGACGAATGGTTCAAGCAGGATTACTCTCCTGTAAATCCTGATGAAGAAGAAGAAGATAAAAACGTCGATGATGAAGCTTTCTCAATACATGAAGTGCCATCTGAGGAGGAGAGGAGTCCAGACGCCCAGCACCCATACACGCATATCAATGCCTTTCAGTTGATCGGAATGTCCTCATGTCTAGACCTCTCCGGCTTCTTTGAGAAAGAGGATGTGTCTGAGAGGAAGATTAGATTTACGTCGAACCACTCTGCTAAAGATTTGCTCGAGAAAATCGAAGAAATTGTATTGGAGATGGGATATTGTGTCCAGAAGAAAAATGGAAGGTTGAAAGTGATGGAAGAACACAAGGGGGAGAGAAGTGCGTGCAGTCTATCAGTTGCAGCAGAGGTGTTTGAGATAAGCCCATCTTTATTTGTAGTAGAGTTGAGAAAATCATGTGGTGATTCTTCTGCATATAGACAGTTGTGTAAAAAGCTTTCAAATGATTTAGGTGTTCCCTCAAGCCAAGAGTTGCTGACTAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018107 GO:0018193 GO:0018209 GO:0018210 GO:0019538 GO:0023052 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0071944 GO:0097305 GO:0140096 GO:1901564 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko04068 ko04140 ko04150 ko04151 ko04152 ko04211 ko04213 ko04371 ko04530 ko04710 ko04714 ko04910 ko04920 ko04921 ko04922 ko04931 ko04932 ko05410 ko05418 map04068 map04140 map04150 map04151 map04152 map04211 map04213 map04371 map04530 map04710 map04714 map04910 map04920 map04921 map04922 map04931 map04932 map05410 map05418

Pfam Domains

Protein Families

KA1 NAF Pkinase

Protein Analysis

447

Amino Acids

49.93

Weight (kDa)

6.06

Isoelectric Point (pI)

41.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	20 - 275	1.4e-72	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	21 - 269	1.8e-40	Protein tyrosine and serine/threonine kinase
NAF	PF03822	318 - 376	6.1e-20	NAF domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003218)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G48260 AT1G48260 AT1G48260 AT1G48260 AT1G48260 AT3G17510 AT3G17510
fragaria_vesca	FvH4_1g17990 FvH4_4g21530 FvH4_4g21530 FvH4_4g21530
malus_domestica	MD13G1139700.v1.1 MD16G1136100.v1.1
prunus_persica	Prupe.1G105000_v2.0.a1 Prupe.1G105000_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0108461 RchiOBHm_Chr4g0427571
rosa_laevigata	RLG00000007224 RLG00000017671
rosa_multiflora	Rmu_co8240981.1_g000001 Rmu_sc0002372.1_g000045 Rmu_sc0009208.1_g000029 Rmu_sc0009627.1_g000006
rosa_roxburghii	Rroxscaffold_2G00135110 Rroxscaffold_5G00369230
rosa_rugosa	Rorug02G0153600 Rorug04G0218700 Rorug04G0218800
rosa_samantha	Rh2BG215200 Rh2CG206400 Rh2DG209500 Rh4AG275200 Rh4BG281100 Rh4CG296300 Rh4DG278400
rosa_wichuraiana	Rw2G016170 Rw4G023860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	596
AciI	CCGC	1 cut(s)	306
AclI	AACGTT	1 cut(s)	237
AclWI	GGATC	2 cut(s)	435, 764
AcoI	YGGCCR	1 cut(s)	127
AcsI	RAATTY	2 cut(s)	104, 179
AcuI	CTGAAG	1 cut(s)	245
AcyI	GRCGYC	2 cut(s)	414, 936
AdeI	CACNNNGTG	1 cut(s)	740
AfeI	AGCGCT	1 cut(s)	496
AfiI	CCNNNNNNNGG	2 cut(s)	360, 612
AflII	CTTAAG	1 cut(s)	431
AgsI	TTSAA	4 cut(s)	217, 826, 1144, 1296
AjnI	CCWGG	1 cut(s)	504
AloI	GAACNNNNNNTCC	2 cut(s)	107, 139
AluBI	AGCT	7 cut(s)	64, 367, 396, 436, 890, 1291, 1338
AluI	AGCT	7 cut(s)	64, 367, 396, 436, 890, 1291, 1338
Alw21I	GWGCWC	1 cut(s)	66
Alw26I	GTCTC	2 cut(s)	344, 421
AlwI	GGATC	2 cut(s)	435, 764
Ama87I	CYCGRG	2 cut(s)	566, 1079
Aor51HI	AGCGCT	1 cut(s)	496
AoxI	GGCC	2 cut(s)	127, 487
ApeKI	GCWGC	1 cut(s)	1196
ApoI	RAATTY	2 cut(s)	104, 179
AseI	ATTAAT	1 cut(s)	380
Asp700I	GAANNNNTTC	1 cut(s)	368
AspLEI	GCGC	1 cut(s)	497
AspS9I	GGNCC	1 cut(s)	286
AsuC2I	CCSGG	1 cut(s)	738
AsuHPI	GGTGA	5 cut(s)	16, 175, 637, 726, 1268
AvaI	CYCGRG	2 cut(s)	566, 1079
AvaII	GGWCC	1 cut(s)	286
BanI	GGYRCC	1 cut(s)	596
BanII	GRGCYC	1 cut(s)	66
BbsI	GAAGAC	1 cut(s)	164
Bbv12I	GWGCWC	1 cut(s)	66
BbvI	GCAGC	1 cut(s)	1208
BccI	CCATC	7 cut(s)	429, 515, 587, 919, 1102, 1144, 1228
BceAI	ACGGC	1 cut(s)	125
BcgI	CGANNNNNNTGC	2 cut(s)	557, 591
BciT130I	CCWGG	1 cut(s)	506
BcnI	CCSGG	1 cut(s)	738
BcoDI	GTCTC	2 cut(s)	344, 421
BfaI	CTAG	2 cut(s)	995, 1335
BfoI	RGCGCY	1 cut(s)	498
BfrI	CTTAAG	1 cut(s)	431
BglII	AGATCT	1 cut(s)	696
BisI	GCNGC	2 cut(s)	307, 1197
BlsI	GCNGC	2 cut(s)	308, 1198
Bme1390I	CCNGG	2 cut(s)	506, 738
Bme18I	GGWCC	1 cut(s)	286
BmeT110I	CYCGRG	2 cut(s)	566, 1079
BmgT120I	GGNCC	1 cut(s)	286
BmiI	GGNNCC	1 cut(s)	598
BmrFI	CCNGG	2 cut(s)	506, 738
BmrI	ACTGGG	1 cut(s)	59
BmsI	GCATC	3 cut(s)	238, 445, 813
BmuI	ACTGGG	1 cut(s)	59
BpiI	GAAGAC	1 cut(s)	164
BpmI	CTGGAG	2 cut(s)	173, 458
Bpu10I	CCTNAGC	2 cut(s)	459, 491
BpuEI	CTTGAG	2 cut(s)	740, 1300
BpuMI	CCSGG	1 cut(s)	738
BsaBI	GATNNNNATC	1 cut(s)	672
BsaHI	GRCGYC	2 cut(s)	414, 936
BsaI	GGTCTC	1 cut(s)	344
BsaJI	CCNNGG	1 cut(s)	504
BsaWI	WCCGGW	1 cut(s)	647
Bsc4I	CCNNNNNNNGG	2 cut(s)	360, 612
Bse1I	ACTGG	4 cut(s)	54, 130, 307, 805
Bse8I	GATNNNNATC	1 cut(s)	672
BseBI	CCWGG	1 cut(s)	506
BseDI	CCNNGG	1 cut(s)	504
BseGI	GGATG	2 cut(s)	229, 1030
BseJI	GATNNNNATC	1 cut(s)	672
BseLI	CCNNNNNNNGG	2 cut(s)	360, 612
BseMII	CTCAG	2 cut(s)	906, 1023
BseNI	ACTGG	4 cut(s)	54, 130, 307, 805
BseRI	GAGGAG	3 cut(s)	932, 935, 940
BseXI	GCAGC	1 cut(s)	1208
BseYI	CCCAGC	1 cut(s)	939
BsgI	GTGCAG	1 cut(s)	1201
BshFI	GGCC	2 cut(s)	129, 489
BshNI	GGYRCC	1 cut(s)	596
BsiHKAI	GWGCWC	1 cut(s)	66
BsiHKCI	CYCGRG	2 cut(s)	566, 1079
BsiSI	CCGG	4 cut(s)	126, 648, 738, 1005
BslI	CCNNNNNNNGG	2 cut(s)	360, 612
BsmAI	GTCTC	2 cut(s)	344, 421
BsmBI	CGTCTC	1 cut(s)	421
BsmI	GAATGC	1 cut(s)	48
BsnI	GGCC	2 cut(s)	129, 489
Bso31I	GGTCTC	1 cut(s)	344
BsoBI	CYCGRG	2 cut(s)	566, 1079
Bsp1286I	GDGCHC	1 cut(s)	66
Bsp143I	GATC	5 cut(s)	6, 427, 696, 769, 975
BspACI	CCGC	1 cut(s)	306
BspANI	GGCC	2 cut(s)	129, 489
BspCNI	CTCAG	2 cut(s)	907, 1024
BspLI	GGNNCC	1 cut(s)	598
BspPI	GGATC	2 cut(s)	435, 764
BspT107I	GGYRCC	1 cut(s)	596
BspTI	CTTAAG	1 cut(s)	431
BspTNI	GGTCTC	1 cut(s)	344
BsrI	ACTGG	4 cut(s)	54, 130, 307, 805
BssECI	CCNNGG	1 cut(s)	504
BssMI	GATC	5 cut(s)	6, 427, 696, 769, 975
BssNI	GRCGYC	2 cut(s)	414, 936
Bst2UI	CCWGG	1 cut(s)	506
Bst4CI	ACNGT	5 cut(s)	20, 32, 389, 401, 1278
BstACI	GRCGYC	2 cut(s)	414, 936
BstAFI	CTTAAG	1 cut(s)	431
BstC8I	GCNNGC	2 cut(s)	265, 1180
BstDEI	CTNAG	5 cut(s)	111, 459, 491, 915, 1032
BstF5I	GGATG	2 cut(s)	229, 1030
BstH2I	RGCGCY	1 cut(s)	498
BstHHI	GCGC	1 cut(s)	497
BstKTI	GATC	5 cut(s)	9, 430, 699, 772, 978
BstMAI	GTCTC	2 cut(s)	344, 421
BstMBI	GATC	5 cut(s)	6, 427, 696, 769, 975
BstNI	CCWGG	1 cut(s)	506
BstSCI	CCNGG	2 cut(s)	504, 736
BstV1I	GCAGC	1 cut(s)	1208
BstV2I	GAAGAC	1 cut(s)	164
BstX2I	RGATCY	2 cut(s)	427, 696
BstXI	CCANNNNNNTGG	1 cut(s)	819
BstYI	RGATCY	2 cut(s)	427, 696
BsuRI	GGCC	2 cut(s)	129, 489
BtsCI	GGATG	2 cut(s)	229, 1030
Cac8I	GCNNGC	2 cut(s)	265, 1180
CfoI	GCGC	1 cut(s)	497
Cfr13I	GGNCC	1 cut(s)	286
CseI	GACGC	2 cut(s)	403, 944
CviAII	CATG	6 cut(s)	251, 283, 618, 902, 990, 1251
DdeI	CTNAG	5 cut(s)	111, 459, 491, 915, 1032
DpnI	GATC	5 cut(s)	8, 429, 698, 771, 977
DpnII	GATC	5 cut(s)	6, 427, 696, 769, 975
DraIII	CACNNNGTG	1 cut(s)	740
EaeI	YGGCCR	1 cut(s)	127
Ecl136II	GAGCTC	1 cut(s)	64
Eco24I	GRGCYC	1 cut(s)	66
Eco31I	GGTCTC	1 cut(s)	344
Eco32I	GATATC	1 cut(s)	477
Eco47I	GGWCC	1 cut(s)	286
Eco47III	AGCGCT	1 cut(s)	496
Eco53kI	GAGCTC	1 cut(s)	64
Eco57I	CTGAAG	1 cut(s)	245
Eco88I	CYCGRG	2 cut(s)	566, 1079
EcoICRI	GAGCTC	1 cut(s)	64
EcoRII	CCWGG	1 cut(s)	504
EcoRV	GATATC	1 cut(s)	477
EcoT38I	GRGCYC	1 cut(s)	66
Esp3I	CGTCTC	1 cut(s)	421
FaeI	CATG	6 cut(s)	254, 286, 621, 905, 993, 1254
FalI	AAGNNNNNCTT	2 cut(s)	1207, 1239
FatI	CATG	6 cut(s)	250, 282, 617, 901, 989, 1250
Fnu4HI	GCNGC	2 cut(s)	307, 1197
FokI	GGATG	2 cut(s)	216, 1037
FriOI	GRGCYC	1 cut(s)	66
Fsp4HI	GCNGC	2 cut(s)	307, 1197
FspBI	CTAG	2 cut(s)	995, 1335
GlaI	GCGC	1 cut(s)	496
GluI	GCNGC	2 cut(s)	307, 1197
GsaI	CCCAGC	1 cut(s)	943
GsuI	CTGGAG	2 cut(s)	173, 458
HaeII	RGCGCY	1 cut(s)	498
HaeIII	GGCC	2 cut(s)	129, 489
HapII	CCGG	4 cut(s)	126, 648, 738, 1005
HgaI	GACGC	2 cut(s)	403, 944
HhaI	GCGC	1 cut(s)	497
Hin1I	GRCGYC	2 cut(s)	414, 936
Hin1II	CATG	6 cut(s)	254, 286, 621, 905, 993, 1254
Hin6I	GCGC	1 cut(s)	495
HinP1I	GCGC	1 cut(s)	495
HindIII	AAGCTT	3 cut(s)	365, 888, 1289
HinfI	GANTC	6 cut(s)	122, 148, 571, 763, 928, 1259
HpaII	CCGG	4 cut(s)	126, 648, 738, 1005
HphI	GGTGA	5 cut(s)	16, 175, 637, 726, 1268
Hpy188I	TCNGA	8 cut(s)	68, 245, 481, 607, 693, 916, 980, 1033
Hpy99I	CGWCG	4 cut(s)	122, 389, 881, 1057
HpyAV	CCTTC	3 cut(s)	63, 350, 1131
HpyCH4III	ACNGT	5 cut(s)	20, 32, 389, 401, 1278
HpyCH4IV	ACGT	5 cut(s)	117, 237, 636, 876, 1052
HpyCH4V	TGCA	6 cut(s)	329, 680, 743, 1182, 1196, 1268
HpyF3I	CTNAG	5 cut(s)	111, 459, 491, 915, 1032
HpySE526I	ACGT	5 cut(s)	117, 237, 636, 876, 1052
Hsp92I	GRCGYC	2 cut(s)	414, 936
Hsp92II	CATG	6 cut(s)	254, 286, 621, 905, 993, 1254
HspAI	GCGC	1 cut(s)	495
Kzo9I	GATC	5 cut(s)	6, 427, 696, 769, 975
LmnI	GCTCC	1 cut(s)	568
Lsp1109I	GCAGC	1 cut(s)	1208
LweI	GCATC	3 cut(s)	238, 445, 813
MaeI	CTAG	2 cut(s)	995, 1335
MaeII	ACGT	5 cut(s)	117, 237, 636, 876, 1052
MaeIII	GTNAC	3 cut(s)	32, 298, 401
MalI	GATC	5 cut(s)	8, 429, 698, 771, 977
MboI	GATC	5 cut(s)	6, 427, 696, 769, 975
MflI	RGATCY	2 cut(s)	427, 696
MhlI	GDGCHC	1 cut(s)	66
MluCI	AATT	9 cut(s)	25, 88, 104, 165, 179, 311, 324, 377, 1095
MlyI	GAGTC	2 cut(s)	131, 937
MmeI	TCCRAC	1 cut(s)	397
MroXI	GAANNNNTTC	1 cut(s)	368
MseI	TTAA	5 cut(s)	144, 177, 380, 432, 701
MslI	CAYNNNNRTG	1 cut(s)	960
MspCI	CTTAAG	1 cut(s)	431
MspI	CCGG	4 cut(s)	126, 648, 738, 1005
MspR9I	CCNGG	2 cut(s)	506, 738
Mva1269I	GAATGC	1 cut(s)	48
MvaI	CCWGG	1 cut(s)	506
NciI	CCSGG	1 cut(s)	738
NdeII	GATC	5 cut(s)	6, 427, 696, 769, 975
NlaIII	CATG	6 cut(s)	254, 286, 621, 905, 993, 1254
NlaIV	GGNNCC	1 cut(s)	598
NmuCI	GTSAC	1 cut(s)	401
PaeR7I	CTCGAG	1 cut(s)	1079
PcsI	WCGNNNNNNNCGW	1 cut(s)	1086
PctI	GAATGC	1 cut(s)	48
PdmI	GAANNNNTTC	1 cut(s)	368
PfeI	GAWTC	4 cut(s)	148, 571, 763, 1259
PkrI	GCNGC	2 cut(s)	308, 1198
PleI	GAGTC	2 cut(s)	130, 936
PpsI	GAGTC	2 cut(s)	130, 936
PshBI	ATTAAT	1 cut(s)	380
Psp124BI	GAGCTC	1 cut(s)	66
Psp1406I	AACGTT	1 cut(s)	237
Psp6I	CCWGG	1 cut(s)	504
PspFI	CCCAGC	1 cut(s)	939
PspGI	CCWGG	1 cut(s)	504
PspN4I	GGNNCC	1 cut(s)	598
PspPI	GGNCC	1 cut(s)	286
PsuI	RGATCY	2 cut(s)	427, 696
RseI	CAYNNNNRTG	1 cut(s)	960
SacI	GAGCTC	1 cut(s)	66
SaqAI	TTAA	5 cut(s)	144, 177, 380, 432, 701
SatI	GCNGC	2 cut(s)	307, 1197
Sau3AI	GATC	5 cut(s)	6, 427, 696, 769, 975
Sau96I	GGNCC	1 cut(s)	286
SchI	GAGTC	2 cut(s)	131, 937
ScrFI	CCNGG	2 cut(s)	506, 738
SduI	GDGCHC	1 cut(s)	66
SfaNI	GCATC	3 cut(s)	238, 445, 813
Sfr274I	CTCGAG	1 cut(s)	1079
SinI	GGWCC	1 cut(s)	286
SlaI	CTCGAG	1 cut(s)	1079
SmiMI	CAYNNNNRTG	1 cut(s)	960
SmlI	CTYRAG	4 cut(s)	431, 755, 1079, 1315
SmoI	CTYRAG	4 cut(s)	431, 755, 1079, 1315
Sse9I	AATT	9 cut(s)	25, 88, 104, 165, 179, 311, 324, 377, 1095
SsiI	CCGC	1 cut(s)	306
SspMI	CTAG	2 cut(s)	995, 1335
SstI	GAGCTC	1 cut(s)	66
StyD4I	CCNGG	2 cut(s)	504, 736
TaaI	ACNGT	5 cut(s)	20, 32, 389, 401, 1278
TaiI	ACGT	5 cut(s)	120, 240, 639, 879, 1055
TaqII	GACCGA	1 cut(s)	368
TasI	AATT	9 cut(s)	25, 88, 104, 165, 179, 311, 324, 377, 1095
TauI	GCSGC	1 cut(s)	309
TfiI	GAWTC	4 cut(s)	148, 571, 763, 1259
Tru1I	TTAA	5 cut(s)	144, 177, 380, 432, 701
Tru9I	TTAA	5 cut(s)	144, 177, 380, 432, 701
TseFI	GTSAC	1 cut(s)	401
TseI	GCWGC	1 cut(s)	1196
Tsp45I	GTSAC	1 cut(s)	401
TspDTI	ATGAA	4 cut(s)	521, 870, 900, 918
TspGWI	ACGGA	1 cut(s)	351
Vha464I	CTTAAG	1 cut(s)	431
VpaK11BI	GGWCC	1 cut(s)	286
VspI	ATTAAT	1 cut(s)	380
XapI	RAATTY	2 cut(s)	104, 179
XbaI	TCTAGA	1 cut(s)	994
XcmI	CCANNNNNNNNNTGG	2 cut(s)	280, 1130
XhoI	CTCGAG	1 cut(s)	1079
XmnI	GAANNNNTTC	1 cut(s)	368
XspI	CTAG	2 cut(s)	995, 1335

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.