MD16G1194400.v1.1

prefoldin subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0003635
Physical Location & Seq
Forward (+)
17238617 .. 17241273
2657 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1194400.v1.1.491

Sequence Viewer

Length: 468 bp
ATGCGATATACTGGAATCATGGCCACTAGTAGAGCTGAAGGGGATCAAAAGGAATATGTAAACGAACAAGCCGTCGCAAATAAGTTTGCTGATATGAGGTCTGAGCTCAACCAAATCTACTCGAAAATTACCGAGCTAGAGATGGAAGCGAGCGAGCACTCACTGGTCATCAATGCCATCCAGCCACTTGATCCGTCCAGGCGATGCTACCGGATGATCGGAGGTGTTCTGGTGGAGAGAACTATCAAGGAGGTCCTGCCAGCTGTGCAGCGCAACAAAGAAGGTATTGAGGAGGTTATTGCCAGGCTGAATGAGGCATTGGAGAAGAAGAAAAAGGAAATCTCTGATTTTGAGGCAAAGTACAAGATCAGGATACGGAAGGAGCAGGGTGAGGAGAAGGATGATAGCGCTCGGAAAGAAGGGACTGCTCAGGGAGTCCTGGTTGGCCCTGCCGGTGGAAGCGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006457 GO:0008150 GO:0009987 GO:0044183 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prefoldin_2

Protein Analysis

156

Amino Acids

17.4

Weight (kDa)

6.02

Isoelectric Point (pI)

37.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prefoldin_2 PF01920 23 - 127 3.5e-27 Prefoldin subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 462
AclWI GGATC 2 cut(s) 51, 185
AcoI YGGCCR 1 cut(s) 21
AcuI CTGAAG 1 cut(s) 57
AfaI GTAC 1 cut(s) 362
AfeI AGCGCT 1 cut(s) 409
AfiI CCNNNNNNNGG 1 cut(s) 455
AhlI ACTAGT 1 cut(s) 26
AjnI CCWGG 3 cut(s) 197, 302, 438
AjuI GAANNNNNNNTTGG 2 cut(s) 302, 334
AluBI AGCT 4 cut(s) 35, 106, 136, 263
AluI AGCT 4 cut(s) 35, 106, 136, 263
Alw21I GWGCWC 2 cut(s) 108, 159
AlwI GGATC 2 cut(s) 51, 185
Aor51HI AGCGCT 1 cut(s) 409
AoxI GGCC 2 cut(s) 21, 445
ApeKI GCWGC 1 cut(s) 268
ArsI GACNNNNNNTTYG 2 cut(s) 57, 89
AspLEI GCGC 2 cut(s) 273, 410
AspS9I GGNCC 2 cut(s) 253, 446
AsuHPI GGTGA 1 cut(s) 401
AvaII GGWCC 1 cut(s) 253
BalI TGGCCA 1 cut(s) 23
BanII GRGCYC 1 cut(s) 108
Bbv12I GWGCWC 2 cut(s) 108, 159
BbvI GCAGC 1 cut(s) 280
BccI CCATC 2 cut(s) 136, 185
BceAI ACGGC 1 cut(s) 56
BciT130I CCWGG 3 cut(s) 199, 304, 440
BciVI GTATCC 1 cut(s) 366
BcuI ACTAGT 1 cut(s) 26
BfaI CTAG 2 cut(s) 27, 137
BfoI RGCGCY 1 cut(s) 411
BfuI GTATCC 1 cut(s) 366
BisI GCNGC 1 cut(s) 269
BlsI GCNGC 1 cut(s) 270
Bme1390I CCNGG 3 cut(s) 199, 304, 440
Bme18I GGWCC 1 cut(s) 253
BmgT120I GGNCC 2 cut(s) 253, 446
BmrFI CCNGG 3 cut(s) 199, 304, 440
BmsI GCATC 1 cut(s) 194
Bpu10I CCTNAGC 1 cut(s) 429
BsaWI WCCGGW 1 cut(s) 210
BsaXI ACNNNNNCTCC 2 cut(s) 426, 456
Bsc4I CCNNNNNNNGG 1 cut(s) 455
Bse118I RCCGGY 1 cut(s) 452
Bse1I ACTGG 2 cut(s) 16, 168
BseBI CCWGG 3 cut(s) 199, 304, 440
BseGI GGATG 3 cut(s) 177, 219, 406
BseLI CCNNNNNNNGG 1 cut(s) 455
BseMII CTCAG 2 cut(s) 93, 443
BseNI ACTGG 2 cut(s) 16, 168
BseRI GAGGAG 2 cut(s) 305, 407
BseXI GCAGC 1 cut(s) 280
BsgI GTGCAG 1 cut(s) 287
BshFI GGCC 2 cut(s) 23, 447
BsiHKAI GWGCWC 2 cut(s) 108, 159
BsiSI CCGG 2 cut(s) 211, 453
BslFI GGGAC 1 cut(s) 436
BslI CCNNNNNNNGG 1 cut(s) 455
BsmFI GGGAC 1 cut(s) 436
BsnI GGCC 2 cut(s) 23, 447
Bsp1286I GDGCHC 2 cut(s) 108, 159
Bsp143I GATC 4 cut(s) 43, 190, 216, 366
BspACI CCGC 1 cut(s) 462
BspANI GGCC 2 cut(s) 23, 447
BspCNI CTCAG 2 cut(s) 94, 442
BspPI GGATC 2 cut(s) 51, 185
BsrFI RCCGGY 1 cut(s) 452
BsrI ACTGG 2 cut(s) 16, 168
BssAI RCCGGY 1 cut(s) 452
BssMI GATC 4 cut(s) 43, 190, 216, 366
Bst2UI CCWGG 3 cut(s) 199, 304, 440
BstC8I GCNNGC 3 cut(s) 151, 155, 261
BstDEI CTNAG 2 cut(s) 102, 429
BstF5I GGATG 3 cut(s) 177, 219, 406
BstH2I RGCGCY 1 cut(s) 411
BstHHI GCGC 2 cut(s) 273, 410
BstKTI GATC 4 cut(s) 46, 193, 219, 369
BstMBI GATC 4 cut(s) 43, 190, 216, 366
BstMWI GCNNNNNNNGC 1 cut(s) 265
BstNI CCWGG 3 cut(s) 199, 304, 440
BstSCI CCNGG 3 cut(s) 197, 302, 438
BstV1I GCAGC 1 cut(s) 280
BsuI GTATCC 1 cut(s) 366
BsuRI GGCC 2 cut(s) 23, 447
BtgZI GCGATG 1 cut(s) 217
BtsCI GGATG 3 cut(s) 177, 219, 406
BtsIMutI CAGTG 1 cut(s) 161
Cac8I GCNNGC 3 cut(s) 151, 155, 261
CfoI GCGC 2 cut(s) 273, 410
Cfr10I RCCGGY 1 cut(s) 452
Cfr13I GGNCC 2 cut(s) 253, 446
Csp6I GTAC 1 cut(s) 361
CviAII CATG 1 cut(s) 19
CviJI RGCY 9 cut(s) 23, 35, 71, 106, 136, 184, 263, 307, 447
CviKI_1 RGCY 9 cut(s) 23, 35, 71, 106, 136, 184, 263, 307, 447
CviQI GTAC 1 cut(s) 361
DdeI CTNAG 2 cut(s) 102, 429
DpnI GATC 4 cut(s) 45, 192, 218, 368
DpnII GATC 4 cut(s) 43, 190, 216, 366
EaeI YGGCCR 1 cut(s) 21
Ecl136II GAGCTC 1 cut(s) 106
Eco24I GRGCYC 1 cut(s) 108
Eco47I GGWCC 1 cut(s) 253
Eco47III AGCGCT 1 cut(s) 409
Eco53kI GAGCTC 1 cut(s) 106
Eco57I CTGAAG 1 cut(s) 57
EcoICRI GAGCTC 1 cut(s) 106
EcoO109I RGGNCCY 1 cut(s) 253
EcoRII CCWGG 3 cut(s) 197, 302, 438
EcoT38I GRGCYC 1 cut(s) 108
FaeI CATG 1 cut(s) 22
FaiI YATR 4 cut(s) 9, 20, 57, 95
FaqI GGGAC 1 cut(s) 436
FatI CATG 1 cut(s) 18
Fnu4HI GCNGC 1 cut(s) 269
FokI GGATG 3 cut(s) 164, 226, 413
FriOI GRGCYC 1 cut(s) 108
Fsp4HI GCNGC 1 cut(s) 269
FspBI CTAG 2 cut(s) 27, 137
GlaI GCGC 2 cut(s) 272, 409
GluI GCNGC 1 cut(s) 269
HaeII RGCGCY 1 cut(s) 411
HaeIII GGCC 2 cut(s) 23, 447
HapII CCGG 2 cut(s) 211, 453
HhaI GCGC 2 cut(s) 273, 410
Hin1II CATG 1 cut(s) 22
Hin6I GCGC 2 cut(s) 271, 408
HinP1I GCGC 2 cut(s) 271, 408
HinfI GANTC 2 cut(s) 15, 435
HpaII CCGG 2 cut(s) 211, 453
HphI GGTGA 1 cut(s) 401
Hpy166II GTNNAC 1 cut(s) 61
Hpy188I TCNGA 4 cut(s) 103, 221, 346, 414
Hpy188III TCNNGA 1 cut(s) 370
Hpy8I GTNNAC 1 cut(s) 61
Hpy99I CGWCG 1 cut(s) 77
HpyAV CCTTC 5 cut(s) 32, 275, 373, 391, 413
HpyCH4V TGCA 1 cut(s) 268
HpyF10VI GCNNNNNNNGC 1 cut(s) 265
HpyF3I CTNAG 2 cut(s) 102, 429
Hsp92II CATG 1 cut(s) 22
HspAI GCGC 2 cut(s) 271, 408
Kzo9I GATC 4 cut(s) 43, 190, 216, 366
LmnI GCTCC 1 cut(s) 382
Lsp1109I GCAGC 1 cut(s) 280
LweI GCATC 1 cut(s) 194
MaeI CTAG 2 cut(s) 27, 137
MalI GATC 4 cut(s) 45, 192, 218, 368
MboI GATC 4 cut(s) 43, 190, 216, 366
MboII GAAGA 2 cut(s) 337, 340
MhlI GDGCHC 2 cut(s) 108, 159
MlsI TGGCCA 1 cut(s) 23
MluCI AATT 1 cut(s) 126
MluNI TGGCCA 1 cut(s) 23
MlyI GAGTC 1 cut(s) 444
MnlI CCTC 8 cut(s) 90, 215, 244, 283, 286, 307, 346, 385
Mox20I TGGCCA 1 cut(s) 23
MscI TGGCCA 1 cut(s) 23
Msp20I TGGCCA 1 cut(s) 23
MspA1I CMGCKG 1 cut(s) 263
MspI CCGG 2 cut(s) 211, 453
MspR9I CCNGG 3 cut(s) 199, 304, 440
MvaI CCWGG 3 cut(s) 199, 304, 440
MwoI GCNNNNNNNGC 1 cut(s) 265
NdeII GATC 4 cut(s) 43, 190, 216, 366
NlaIII CATG 1 cut(s) 22
PcsI WCGNNNNNNNCGW 1 cut(s) 69
PfeI GAWTC 1 cut(s) 15
PkrI GCNGC 1 cut(s) 270
PleI GAGTC 1 cut(s) 443
PpsI GAGTC 1 cut(s) 443
PpuMI RGGWCCY 1 cut(s) 253
Psp124BI GAGCTC 1 cut(s) 108
Psp5II RGGWCCY 1 cut(s) 253
Psp6I CCWGG 3 cut(s) 197, 302, 438
PspGI CCWGG 3 cut(s) 197, 302, 438
PspPI GGNCC 2 cut(s) 253, 446
PspPPI RGGWCCY 1 cut(s) 253
PvuII CAGCTG 1 cut(s) 263
RsaI GTAC 1 cut(s) 362
RsaNI GTAC 1 cut(s) 361
SacI GAGCTC 1 cut(s) 108
SatI GCNGC 1 cut(s) 269
Sau3AI GATC 4 cut(s) 43, 190, 216, 366
Sau96I GGNCC 2 cut(s) 253, 446
SchI GAGTC 1 cut(s) 444
ScrFI CCNGG 3 cut(s) 199, 304, 440
SduI GDGCHC 2 cut(s) 108, 159
SetI ASST 9 cut(s) 37, 101, 108, 138, 226, 255, 265, 286, 297
SfaNI GCATC 1 cut(s) 194
SinI GGWCC 1 cut(s) 253
SpeI ACTAGT 1 cut(s) 26
Sse9I AATT 1 cut(s) 126
SsiI CCGC 1 cut(s) 462
SspMI CTAG 2 cut(s) 27, 137
SstI GAGCTC 1 cut(s) 108
StyD4I CCNGG 3 cut(s) 197, 302, 438
TaqI TCGA 1 cut(s) 122
TasI AATT 1 cut(s) 126
TatI WGTACW 1 cut(s) 360
TfiI GAWTC 1 cut(s) 15
TscAI CASTG 1 cut(s) 168
TseI GCWGC 1 cut(s) 268
TspGWI ACGGA 2 cut(s) 183, 391
TspRI CASTG 1 cut(s) 168
VpaK11BI GGWCC 1 cut(s) 253
XspI CTAG 2 cut(s) 27, 137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.