pycom16g16420

prefoldin subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0003635
Physical Location & Seq
Forward (+)
12340907 .. 12341281
375 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g16420.1

Sequence Viewer

Length: 375 bp
ATGAGGTCTGAGCTCAACCAAATCTACTCGAAAATTACTGAGCTAGAGATGGAAGCGAGCGAGCACTCACTGGTCATCAATGCCATCCAGCCACTTGATCCGTCCAGGCGATGCTACCGGATGATTGGAGGTGTTCTGGTGGAGAGAACCATCAAGGAGGTCCTGCCAGCCGTGCAGCGCAACAAAGAAGGTATTGAGGAGGTTATTGCCAGGCTGAATGAGGCATTGGAGAAGAAGAAAAAGGAAATCTCTGATTTTGAGGCTAAGTACAAGATCAGGATACGGAAGGAGCAGGGTGAGGAGAAGGATGATAGCGCTAGGAAAGAAGGGACTGCTCAGGGAGTCCTGGTTGGCCCTGCCGGTGGAAGCGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006457 GO:0008150 GO:0009987 GO:0044183 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prefoldin_2

Protein Analysis

125

Amino Acids

13.92

Weight (kDa)

6.63

Isoelectric Point (pI)

41.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prefoldin_2 PF01920 2 - 96 3e-24 Prefoldin subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 369
AclWI GGATC 1 cut(s) 92
AfaI GTAC 1 cut(s) 269
AfeI AGCGCT 1 cut(s) 316
AfiI CCNNNNNNNGG 1 cut(s) 362
AjnI CCWGG 3 cut(s) 104, 209, 345
AjuI GAANNNNNNNTTGG 2 cut(s) 209, 241
AluBI AGCT 2 cut(s) 13, 43
AluI AGCT 2 cut(s) 13, 43
Alw21I GWGCWC 2 cut(s) 15, 66
AlwI GGATC 1 cut(s) 92
Aor51HI AGCGCT 1 cut(s) 316
AoxI GGCC 1 cut(s) 352
ApeKI GCWGC 1 cut(s) 175
AspLEI GCGC 2 cut(s) 180, 317
AspS9I GGNCC 2 cut(s) 160, 353
AsuHPI GGTGA 1 cut(s) 308
AvaII GGWCC 1 cut(s) 160
BanII GRGCYC 1 cut(s) 15
Bbv12I GWGCWC 2 cut(s) 15, 66
BbvI GCAGC 1 cut(s) 187
BccI CCATC 3 cut(s) 43, 92, 158
BceAI ACGGC 1 cut(s) 155
BciT130I CCWGG 3 cut(s) 106, 211, 347
BciVI GTATCC 1 cut(s) 273
BfaI CTAG 2 cut(s) 44, 318
BfoI RGCGCY 1 cut(s) 318
BfuI GTATCC 1 cut(s) 273
BisI GCNGC 1 cut(s) 176
BlsI GCNGC 1 cut(s) 177
Bme1390I CCNGG 3 cut(s) 106, 211, 347
Bme18I GGWCC 1 cut(s) 160
BmgT120I GGNCC 2 cut(s) 160, 353
BmrFI CCNGG 3 cut(s) 106, 211, 347
BmsI GCATC 1 cut(s) 101
Bpu10I CCTNAGC 1 cut(s) 336
BsaWI WCCGGW 1 cut(s) 117
BsaXI ACNNNNNCTCC 2 cut(s) 333, 363
Bsc4I CCNNNNNNNGG 1 cut(s) 362
Bse118I RCCGGY 1 cut(s) 359
Bse1I ACTGG 1 cut(s) 75
BseBI CCWGG 3 cut(s) 106, 211, 347
BseGI GGATG 3 cut(s) 84, 126, 313
BseLI CCNNNNNNNGG 1 cut(s) 362
BseMII CTCAG 2 cut(s) 30, 350
BseNI ACTGG 1 cut(s) 75
BseRI GAGGAG 2 cut(s) 212, 314
BseXI GCAGC 1 cut(s) 187
BsgI GTGCAG 1 cut(s) 194
BshFI GGCC 1 cut(s) 354
BsiHKAI GWGCWC 2 cut(s) 15, 66
BsiSI CCGG 2 cut(s) 118, 360
BslFI GGGAC 1 cut(s) 343
BslI CCNNNNNNNGG 1 cut(s) 362
BsmFI GGGAC 1 cut(s) 343
BsnI GGCC 1 cut(s) 354
Bsp1286I GDGCHC 2 cut(s) 15, 66
Bsp143I GATC 2 cut(s) 97, 273
BspACI CCGC 1 cut(s) 369
BspANI GGCC 1 cut(s) 354
BspCNI CTCAG 2 cut(s) 31, 349
BspPI GGATC 1 cut(s) 92
BsrFI RCCGGY 1 cut(s) 359
BsrI ACTGG 1 cut(s) 75
BssAI RCCGGY 1 cut(s) 359
BssMI GATC 2 cut(s) 97, 273
Bst2UI CCWGG 3 cut(s) 106, 211, 347
BstC8I GCNNGC 3 cut(s) 58, 62, 168
BstDEI CTNAG 4 cut(s) 9, 39, 264, 336
BstF5I GGATG 3 cut(s) 84, 126, 313
BstH2I RGCGCY 1 cut(s) 318
BstHHI GCGC 2 cut(s) 180, 317
BstKTI GATC 2 cut(s) 100, 276
BstMBI GATC 2 cut(s) 97, 273
BstMWI GCNNNNNNNGC 1 cut(s) 172
BstNI CCWGG 3 cut(s) 106, 211, 347
BstSCI CCNGG 3 cut(s) 104, 209, 345
BstV1I GCAGC 1 cut(s) 187
BsuI GTATCC 1 cut(s) 273
BsuRI GGCC 1 cut(s) 354
BtgZI GCGATG 1 cut(s) 124
BtsCI GGATG 3 cut(s) 84, 126, 313
BtsIMutI CAGTG 1 cut(s) 68
Cac8I GCNNGC 3 cut(s) 58, 62, 168
CfoI GCGC 2 cut(s) 180, 317
Cfr10I RCCGGY 1 cut(s) 359
Cfr13I GGNCC 2 cut(s) 160, 353
Csp6I GTAC 1 cut(s) 268
CviJI RGCY 7 cut(s) 13, 43, 91, 170, 214, 263, 354
CviKI_1 RGCY 7 cut(s) 13, 43, 91, 170, 214, 263, 354
CviQI GTAC 1 cut(s) 268
DdeI CTNAG 4 cut(s) 9, 39, 264, 336
DpnI GATC 2 cut(s) 99, 275
DpnII GATC 2 cut(s) 97, 273
Ecl136II GAGCTC 1 cut(s) 13
Eco24I GRGCYC 1 cut(s) 15
Eco47I GGWCC 1 cut(s) 160
Eco47III AGCGCT 1 cut(s) 316
Eco53kI GAGCTC 1 cut(s) 13
EcoICRI GAGCTC 1 cut(s) 13
EcoO109I RGGNCCY 1 cut(s) 160
EcoRII CCWGG 3 cut(s) 104, 209, 345
EcoT38I GRGCYC 1 cut(s) 15
FaqI GGGAC 1 cut(s) 343
Fnu4HI GCNGC 1 cut(s) 176
FokI GGATG 3 cut(s) 71, 133, 320
FriOI GRGCYC 1 cut(s) 15
Fsp4HI GCNGC 1 cut(s) 176
FspBI CTAG 2 cut(s) 44, 318
GlaI GCGC 2 cut(s) 179, 316
GluI GCNGC 1 cut(s) 176
HaeII RGCGCY 1 cut(s) 318
HaeIII GGCC 1 cut(s) 354
HapII CCGG 2 cut(s) 118, 360
HhaI GCGC 2 cut(s) 180, 317
Hin6I GCGC 2 cut(s) 178, 315
HinP1I GCGC 2 cut(s) 178, 315
HinfI GANTC 1 cut(s) 342
HpaII CCGG 2 cut(s) 118, 360
HphI GGTGA 1 cut(s) 308
Hpy188I TCNGA 2 cut(s) 10, 253
Hpy188III TCNNGA 1 cut(s) 277
HpyAV CCTTC 4 cut(s) 182, 280, 298, 320
HpyCH4V TGCA 1 cut(s) 175
HpyF10VI GCNNNNNNNGC 1 cut(s) 172
HpyF3I CTNAG 4 cut(s) 9, 39, 264, 336
HspAI GCGC 2 cut(s) 178, 315
Kzo9I GATC 2 cut(s) 97, 273
LmnI GCTCC 1 cut(s) 289
Lsp1109I GCAGC 1 cut(s) 187
LweI GCATC 1 cut(s) 101
MaeI CTAG 2 cut(s) 44, 318
MalI GATC 2 cut(s) 99, 275
MboI GATC 2 cut(s) 97, 273
MboII GAAGA 2 cut(s) 244, 247
MhlI GDGCHC 2 cut(s) 15, 66
MluCI AATT 1 cut(s) 33
MlyI GAGTC 1 cut(s) 351
MnlI CCTC 7 cut(s) 122, 151, 190, 193, 214, 253, 292
MspI CCGG 2 cut(s) 118, 360
MspR9I CCNGG 3 cut(s) 106, 211, 347
MvaI CCWGG 3 cut(s) 106, 211, 347
MwoI GCNNNNNNNGC 1 cut(s) 172
NdeII GATC 2 cut(s) 97, 273
PkrI GCNGC 1 cut(s) 177
PleI GAGTC 1 cut(s) 350
PpsI GAGTC 1 cut(s) 350
PpuMI RGGWCCY 1 cut(s) 160
Psp124BI GAGCTC 1 cut(s) 15
Psp5II RGGWCCY 1 cut(s) 160
Psp6I CCWGG 3 cut(s) 104, 209, 345
PspGI CCWGG 3 cut(s) 104, 209, 345
PspPI GGNCC 2 cut(s) 160, 353
PspPPI RGGWCCY 1 cut(s) 160
RsaI GTAC 1 cut(s) 269
RsaNI GTAC 1 cut(s) 268
SacI GAGCTC 1 cut(s) 15
SatI GCNGC 1 cut(s) 176
Sau3AI GATC 2 cut(s) 97, 273
Sau96I GGNCC 2 cut(s) 160, 353
SchI GAGTC 1 cut(s) 351
ScrFI CCNGG 3 cut(s) 106, 211, 347
SduI GDGCHC 2 cut(s) 15, 66
SetI ASST 7 cut(s) 8, 15, 45, 133, 162, 193, 204
SfaNI GCATC 1 cut(s) 101
SinI GGWCC 1 cut(s) 160
Sse9I AATT 1 cut(s) 33
SsiI CCGC 1 cut(s) 369
SspMI CTAG 2 cut(s) 44, 318
SstI GAGCTC 1 cut(s) 15
StyD4I CCNGG 3 cut(s) 104, 209, 345
TaqI TCGA 1 cut(s) 29
TasI AATT 1 cut(s) 33
TatI WGTACW 1 cut(s) 267
TscAI CASTG 1 cut(s) 75
TseI GCWGC 1 cut(s) 175
TspGWI ACGGA 2 cut(s) 90, 298
TspRI CASTG 1 cut(s) 75
VpaK11BI GGWCC 1 cut(s) 160
XspI CTAG 2 cut(s) 44, 318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.