MD17G1107200.v1.1

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0004659
Physical Location & Seq
Forward (+)
9230305 .. 9230988
684 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1107200.v1.1.491

Sequence Viewer

Length: 684 bp
ATGCGGCGAGGAGGCAGAGCCACTGCGGCTGCTGCAGCAGCAGCAGGAGTCCAATTATGCAAGCCCAAGACGGCGGTGGCCGCGGCGGTACAGGAAATTAGATTCCGGGGCGTGCGGAAGCGACCGTGGGGCCGATTCGCCGCCGAGATCAGAGACCCGTGGAAGAAGACTCGGGTCTGGCTCGGCACCTTCGACTCGGCCGAGGACGCGGCCCGGGCATATGACGCCGCCGCTCGGTCCCTCCGGGGGCCCAAGGCCAAGACTAATTTCCCTATCCCCGCCGAATACGACAACAACCACCACCACCTACCTCCTCCGTCCCATCTTTCTCTCTACCCAGACGACACGGTTTGCGATGTCGTCCAGGCCAGCCGGCCCACCTCCAGCAGTCTCAGCAGCACCGTCGAGTCCTTCAGTGGGCCCCGCCCCCGGCCTAGGCCCACATCCGACCCCCAGCCGCAGCGGAGACCGGTGCTCCCGGTTTCCCCCGAGGACTGCCACAGCGACTGCGACTCCTCGTCATCTGTCGTTGACGACGGCGATGATTGCGTCCTGATGTCGTCGTTCCGGAGAATCTCGCCCCTGCCGTTCGATCTCAACCTGCCGCCGCCGATGGACGGCCTCGATTTATTCAAATCCGGCGACGACGATTACGATCTTCAAGCCACCGCTTTGTGCCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

228

Amino Acids

24.74

Weight (kDa)

6.95

Isoelectric Point (pI)

64.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 34 - 83 6.1e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 609
AccB1I GGYRCC 1 cut(s) 185
AccBSI CCGCTC 1 cut(s) 233
AccII CGCG 2 cut(s) 83, 209
AccIII TCCGGA 1 cut(s) 567
AcoI YGGCCR 2 cut(s) 78, 198
AcuI CTGAAG 1 cut(s) 397
AcyI GRCGYC 1 cut(s) 225
AfaI GTAC 1 cut(s) 90
AfiI CCNNNNNNNGG 6 cut(s) 234, 246, 417, 429, 435, 617
AgeI ACCGGT 1 cut(s) 469
AgsI TTSAA 2 cut(s) 634, 662
AhdI GACNNNNNGTC 1 cut(s) 517
AjnI CCWGG 1 cut(s) 363
AjuI GAANNNNNNNTTGG 2 cut(s) 251, 283
Alw21I GWGCWC 1 cut(s) 477
Alw26I GTCTC 3 cut(s) 147, 395, 460
AlwNI CAGNNNCTG 1 cut(s) 507
Ama87I CYCGRG 3 cut(s) 171, 213, 488
Aor13HI TCCGGA 1 cut(s) 567
ApaI GGGCCC 2 cut(s) 252, 423
ApeKI GCWGC 7 cut(s) 29, 32, 35, 38, 41, 396, 460
AsiGI ACCGGT 1 cut(s) 469
AspA2I CCTAGG 1 cut(s) 434
AspS9I GGNCC 9 cut(s) 130, 211, 237, 248, 249, 375, 419, 420, 438
AsuC2I CCSGG 6 cut(s) 107, 214, 215, 245, 430, 479
AvaI CYCGRG 3 cut(s) 171, 213, 488
AvaII GGWCC 1 cut(s) 237
AvrII CCTAGG 1 cut(s) 434
BaeGI GKGCMC 2 cut(s) 252, 423
BanI GGYRCC 1 cut(s) 185
BanII GRGCYC 2 cut(s) 252, 423
BbsI GAAGAC 1 cut(s) 173
Bbv12I GWGCWC 1 cut(s) 477
BbvI GCAGC 7 cut(s) 16, 19, 47, 50, 53, 408, 472
BccI CCATC 2 cut(s) 330, 607
BceAI ACGGC 4 cut(s) 87, 553, 571, 634
BcgI CGANNNNNNTGC 2 cut(s) 385, 419
BciT130I CCWGG 1 cut(s) 365
BcnI CCSGG 6 cut(s) 107, 214, 215, 245, 430, 479
BcoDI GTCTC 3 cut(s) 147, 395, 460
BfaI CTAG 1 cut(s) 435
BfmI CTRYAG 1 cut(s) 33
BfuAI ACCTGC 1 cut(s) 609
BglI GCCNNNNNGGC 1 cut(s) 26
BlnI CCTAGG 1 cut(s) 434
Bme1390I CCNGG 7 cut(s) 107, 214, 215, 245, 365, 430, 479
Bme18I GGWCC 1 cut(s) 237
BmeRI GACNNNNNGTC 1 cut(s) 517
BmeT110I CYCGRG 3 cut(s) 171, 213, 488
BmgT120I GGNCC 9 cut(s) 130, 211, 237, 248, 249, 375, 419, 420, 438
BmiI GGNNCC 7 cut(s) 131, 187, 239, 249, 250, 421, 422
BmrFI CCNGG 7 cut(s) 107, 214, 215, 245, 365, 430, 479
BoxI GACNNNNGTC 1 cut(s) 173
BpiI GAAGAC 1 cut(s) 173
BpmI CTGGAG 1 cut(s) 367
BpuMI CCSGG 6 cut(s) 107, 214, 215, 245, 430, 479
BsaBI GATNNNNATC 1 cut(s) 654
BsaHI GRCGYC 1 cut(s) 225
BsaI GGTCTC 2 cut(s) 147, 460
BsaWI WCCGGW 2 cut(s) 469, 567
BsaXI ACNNNNNCTCC 4 cut(s) 459, 489, 497, 527
Bsc4I CCNNNNNNNGG 6 cut(s) 234, 246, 417, 429, 435, 617
Bse118I RCCGGY 2 cut(s) 372, 469
Bse8I GATNNNNATC 1 cut(s) 654
BseAI TCCGGA 1 cut(s) 567
BseBI CCWGG 1 cut(s) 365
BseGI GGATG 1 cut(s) 443
BseJI GATNNNNATC 1 cut(s) 654
BseLI CCNNNNNNNGG 6 cut(s) 234, 246, 417, 429, 435, 617
BseMII CTCAG 1 cut(s) 406
BseRI GAGGAG 3 cut(s) 24, 303, 505
BseSI GKGCMC 2 cut(s) 252, 423
BseX3I CGGCCG 1 cut(s) 198
BseXI GCAGC 7 cut(s) 16, 19, 47, 50, 53, 408, 472
BseYI CCCAGC 1 cut(s) 453
Bsh1236I CGCG 2 cut(s) 83, 209
Bsh1285I CGRYCG 2 cut(s) 125, 201
BshNI GGYRCC 1 cut(s) 185
BshTI ACCGGT 1 cut(s) 469
BsiEI CGRYCG 2 cut(s) 125, 201
BsiHKAI GWGCWC 1 cut(s) 477
BsiHKCI CYCGRG 3 cut(s) 171, 213, 488
BsiSI CCGG 9 cut(s) 106, 214, 244, 373, 430, 470, 479, 568, 639
BslFI GGGAC 2 cut(s) 223, 304
BslI CCNNNNNNNGG 6 cut(s) 234, 246, 417, 429, 435, 617
BsmAI GTCTC 3 cut(s) 147, 395, 460
BsmFI GGGAC 2 cut(s) 223, 304
Bso31I GGTCTC 2 cut(s) 147, 460
BsoBI CYCGRG 3 cut(s) 171, 213, 488
Bsp120I GGGCCC 2 cut(s) 248, 419
Bsp1286I GDGCHC 3 cut(s) 252, 423, 477
Bsp13I TCCGGA 1 cut(s) 567
Bsp143I GATC 3 cut(s) 147, 592, 655
BspCNI CTCAG 1 cut(s) 405
BspEI TCCGGA 1 cut(s) 567
BspFNI CGCG 2 cut(s) 83, 209
BspLI GGNNCC 7 cut(s) 131, 187, 239, 249, 250, 421, 422
BspMAI CTGCAG 1 cut(s) 37
BspMI ACCTGC 1 cut(s) 609
BspT107I GGYRCC 1 cut(s) 185
BspTNI GGTCTC 2 cut(s) 147, 460
BsrBI CCGCTC 1 cut(s) 233
BsrFI RCCGGY 2 cut(s) 372, 469
BssAI RCCGGY 2 cut(s) 372, 469
BssMI GATC 3 cut(s) 147, 592, 655
BssNI GRCGYC 1 cut(s) 225
BssT1I CCWWGG 2 cut(s) 252, 434
Bst2UI CCWGG 1 cut(s) 365
Bst4CI ACNGT 3 cut(s) 126, 349, 403
BstACI GRCGYC 1 cut(s) 225
BstC8I GCNNGC 4 cut(s) 62, 113, 370, 374
BstDEI CTNAG 1 cut(s) 392
BstDSI CCRYGG 3 cut(s) 81, 125, 158
BstF5I GGATG 1 cut(s) 443
BstFNI CGCG 2 cut(s) 83, 209
BstKTI GATC 3 cut(s) 150, 595, 658
BstMAI GTCTC 3 cut(s) 147, 395, 460
BstMBI GATC 3 cut(s) 147, 592, 655
BstMCI CGRYCG 2 cut(s) 125, 201
BstNI CCWGG 1 cut(s) 365
BstPAI GACNNNNGTC 1 cut(s) 173
BstSCI CCNGG 7 cut(s) 105, 212, 213, 243, 363, 428, 477
BstSFI CTRYAG 1 cut(s) 33
BstSLI GKGCMC 2 cut(s) 252, 423
BstUI CGCG 2 cut(s) 83, 209
BstV1I GCAGC 7 cut(s) 16, 19, 47, 50, 53, 408, 472
BstV2I GAAGAC 1 cut(s) 173
BstZI CGGCCG 1 cut(s) 198
BtgI CCRYGG 3 cut(s) 81, 125, 158
BtgZI GCGATG 2 cut(s) 369, 555
BtsCI GGATG 1 cut(s) 443
BtsI GCAGTG 1 cut(s) 21
BtsIMutI CAGTG 2 cut(s) 21, 421
BveI ACCTGC 1 cut(s) 609
Cac8I GCNNGC 4 cut(s) 62, 113, 370, 374
CaiI CAGNNNCTG 1 cut(s) 507
Cfr10I RCCGGY 2 cut(s) 372, 469
Cfr13I GGNCC 9 cut(s) 130, 211, 237, 248, 249, 375, 419, 420, 438
Cfr42I CCGCGG 1 cut(s) 84
Cfr9I CCCGGG 1 cut(s) 213
CseI GACGC 3 cut(s) 215, 233, 538
Csp6I GTAC 1 cut(s) 89
CspAI ACCGGT 1 cut(s) 469
CviQI GTAC 1 cut(s) 89
DdeI CTNAG 1 cut(s) 392
DpnI GATC 3 cut(s) 149, 594, 657
DpnII GATC 3 cut(s) 147, 592, 655
DriI GACNNNNNGTC 1 cut(s) 517
EaeI YGGCCR 2 cut(s) 78, 198
EagI CGGCCG 1 cut(s) 198
Eam1105I GACNNNNNGTC 1 cut(s) 517
EclXI CGGCCG 1 cut(s) 198
Eco130I CCWWGG 2 cut(s) 252, 434
Eco24I GRGCYC 2 cut(s) 252, 423
Eco31I GGTCTC 2 cut(s) 147, 460
Eco47I GGWCC 1 cut(s) 237
Eco52I CGGCCG 1 cut(s) 198
Eco57I CTGAAG 1 cut(s) 397
Eco88I CYCGRG 3 cut(s) 171, 213, 488
EcoO109I RGGNCCY 2 cut(s) 248, 420
EcoRII CCWGG 1 cut(s) 363
EcoT14I CCWWGG 2 cut(s) 252, 434
EcoT38I GRGCYC 2 cut(s) 252, 423
ErhI CCWWGG 2 cut(s) 252, 434
FaiI YATR 3 cut(s) 58, 220, 222
FaqI GGGAC 2 cut(s) 223, 304
FauI CCCGC 2 cut(s) 286, 431
FauNDI CATATG 1 cut(s) 220
FokI GGATG 1 cut(s) 430
FriOI GRGCYC 2 cut(s) 252, 423
FspBI CTAG 1 cut(s) 435
GsaI CCCAGC 1 cut(s) 457
GsuI CTGGAG 1 cut(s) 367
HapII CCGG 9 cut(s) 106, 214, 244, 373, 430, 470, 479, 568, 639
HgaI GACGC 3 cut(s) 215, 233, 538
Hin1I GRCGYC 1 cut(s) 225
HincII GTYRAC 1 cut(s) 532
HindII GTYRAC 1 cut(s) 532
HinfI GANTC 8 cut(s) 48, 102, 135, 169, 194, 407, 512, 573
HpaII CCGG 9 cut(s) 106, 214, 244, 373, 430, 470, 479, 568, 639
Hpy166II GTNNAC 1 cut(s) 532
Hpy188I TCNGA 2 cut(s) 152, 448
Hpy188III TCNNGA 2 cut(s) 553, 568
Hpy8I GTNNAC 1 cut(s) 532
Hpy99I CGWCG 5 cut(s) 407, 539, 565, 647, 650
HpyAV CCTTC 2 cut(s) 199, 421
HpyCH4III ACNGT 3 cut(s) 126, 349, 403
HpyCH4V TGCA 2 cut(s) 35, 60
HpyF3I CTNAG 1 cut(s) 392
Hsp92I GRCGYC 1 cut(s) 225
Kpn2I TCCGGA 1 cut(s) 567
KroI GCCGGC 1 cut(s) 372
KroNI GCCGGC 1 cut(s) 374
KspI CCGCGG 1 cut(s) 84
Kzo9I GATC 3 cut(s) 147, 592, 655
LmnI GCTCC 1 cut(s) 480
Lsp1109I GCAGC 7 cut(s) 16, 19, 47, 50, 53, 408, 472
MaeI CTAG 1 cut(s) 435
MalI GATC 3 cut(s) 149, 594, 657
MbiI CCGCTC 1 cut(s) 233
MboI GATC 3 cut(s) 147, 592, 655
MboII GAAGA 3 cut(s) 175, 178, 650
MhlI GDGCHC 3 cut(s) 252, 423, 477
MluCI AATT 3 cut(s) 53, 96, 265
MlyI GAGTC 5 cut(s) 57, 163, 188, 416, 506
MmeI TCCRAC 1 cut(s) 471
MnlI CCTC 9 cut(s) 5, 196, 251, 321, 324, 391, 484, 526, 632
MroI TCCGGA 1 cut(s) 567
MroNI GCCGGC 1 cut(s) 372
MspA1I CMGCKG 2 cut(s) 83, 463
MspI CCGG 9 cut(s) 106, 214, 244, 373, 430, 470, 479, 568, 639
MspR9I CCNGG 7 cut(s) 107, 214, 215, 245, 365, 430, 479
MvaI CCWGG 1 cut(s) 365
MvnI CGCG 2 cut(s) 83, 209
NaeI GCCGGC 1 cut(s) 374
NciI CCSGG 6 cut(s) 107, 214, 215, 245, 430, 479
NdeI CATATG 1 cut(s) 220
NdeII GATC 3 cut(s) 147, 592, 655
NgoMIV GCCGGC 1 cut(s) 372
NlaIV GGNNCC 7 cut(s) 131, 187, 239, 249, 250, 421, 422
NmeAIII GCCGAG 4 cut(s) 162, 169, 176, 226
PcsI WCGNNNNNNNCGW 4 cut(s) 189, 198, 584, 651
PdiI GCCGGC 1 cut(s) 374
PfeI GAWTC 3 cut(s) 102, 135, 573
PinAI ACCGGT 1 cut(s) 469
PleI GAGTC 5 cut(s) 56, 163, 188, 415, 506
PpsI GAGTC 5 cut(s) 56, 163, 188, 415, 506
PshAI GACNNNNGTC 1 cut(s) 173
Psp6I CCWGG 1 cut(s) 363
PspFI CCCAGC 1 cut(s) 453
PspGI CCWGG 1 cut(s) 363
PspN4I GGNNCC 7 cut(s) 131, 187, 239, 249, 250, 421, 422
PspOMI GGGCCC 2 cut(s) 248, 419
PspPI GGNCC 9 cut(s) 130, 211, 237, 248, 249, 375, 419, 420, 438
PstI CTGCAG 1 cut(s) 37
PstNI CAGNNNCTG 1 cut(s) 507
RsaI GTAC 1 cut(s) 90
RsaNI GTAC 1 cut(s) 89
SacII CCGCGG 1 cut(s) 84
Sau3AI GATC 3 cut(s) 147, 592, 655
Sau96I GGNCC 9 cut(s) 130, 211, 237, 248, 249, 375, 419, 420, 438
SchI GAGTC 5 cut(s) 57, 163, 188, 416, 506
ScrFI CCNGG 7 cut(s) 107, 214, 215, 245, 365, 430, 479
SduI GDGCHC 3 cut(s) 252, 423, 477
SetI ASST 5 cut(s) 191, 309, 313, 383, 603
SfcI CTRYAG 1 cut(s) 33
Sfr303I CCGCGG 1 cut(s) 84
SgrBI CCGCGG 1 cut(s) 84
SinI GGWCC 1 cut(s) 237
SmaI CCCGGG 1 cut(s) 215
SrfI GCCCGGGC 1 cut(s) 215
Sse9I AATT 3 cut(s) 53, 96, 265
SspMI CTAG 1 cut(s) 435
StyD4I CCNGG 7 cut(s) 105, 212, 213, 243, 363, 428, 477
StyI CCWWGG 2 cut(s) 252, 434
TaaI ACNGT 3 cut(s) 126, 349, 403
TaqI TCGA 4 cut(s) 192, 405, 591, 624
TaqII GACCGA 1 cut(s) 225
TasI AATT 3 cut(s) 53, 96, 265
TfiI GAWTC 3 cut(s) 102, 135, 573
TscAI CASTG 2 cut(s) 28, 421
TseI GCWGC 7 cut(s) 29, 32, 35, 38, 41, 396, 460
TspGWI ACGGA 1 cut(s) 306
TspMI CCCGGG 1 cut(s) 213
TspRI CASTG 2 cut(s) 28, 421
VpaK11BI GGWCC 1 cut(s) 237
XcmI CCANNNNNNNNNTGG 1 cut(s) 73
XmaI CCCGGG 1 cut(s) 213
XmaJI CCTAGG 1 cut(s) 434
XspI CTAG 1 cut(s) 435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.