MD17G1160300.v1.1

PB1 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0002525
Physical Location & Seq
Reverse (-)
15670120 .. 15670539
420 bp
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UTR
Exon/CDS
Intron
MD17G1160300.v1.1.491

Sequence Viewer

Length: 420 bp
ATGAAATCGACTGCGACACCCTTTCGTGAGACTACCAAACGCCCTTTCACAACCTCCAACTACAAAGTTAAGTTCATGTGCAGCTACTGCAGCAAAATCCAGCCTCGCCCCCATGACCACCAGCTTGCCTACGTCAGCGGTGACACCAAAATCCTCGTTGTTAATCGCAATATCAAGCTTTTTGTTTTCATCTCTAAGCTCTCCTCCCTCTGTGACACCTCTAATGACGCCGTTTGCTTCAAGTACCAGCTTCCTGGCAAAGATCTTGACATTCTCATATCTGTCACCAACGACGAAGACCTCGACCACATGATGGTCGAGTACGGTCGACTCTACAGCGCTTCCGCTAAGCCTACTAGGCTCCAACTCTTTCTTTTCCCTCTTGACAGCAACACTTCGTGTAGCTTCAGCTCCAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

140

Amino Acids

15.8

Weight (kDa)

8.81

Isoelectric Point (pI)

29.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 39 - 127 1e-12 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 313
AccI GTMKAC 1 cut(s) 328
AciI CCGC 2 cut(s) 138, 345
AcuI CTGAAG 1 cut(s) 391
AcyI GRCGYC 1 cut(s) 228
AdeI CACNNNGTG 1 cut(s) 399
AfaI GTAC 2 cut(s) 245, 323
AfeI AGCGCT 1 cut(s) 340
AfiI CCNNNNNNNGG 1 cut(s) 313
AgsI TTSAA 1 cut(s) 241
AjnI CCWGG 1 cut(s) 253
AjuI GAANNNNNNNTTGG 2 cut(s) 29, 61
AluBI AGCT 8 cut(s) 84, 124, 178, 199, 250, 405, 411, 417
AluI AGCT 8 cut(s) 84, 124, 178, 199, 250, 405, 411, 417
Alw26I GTCTC 1 cut(s) 23
AlwNI CAGNNNCTG 1 cut(s) 87
Aor51HI AGCGCT 1 cut(s) 340
ApeKI GCWGC 2 cut(s) 81, 90
AspLEI GCGC 1 cut(s) 341
AsuHPI GGTGA 2 cut(s) 152, 277
BbsI GAAGAC 1 cut(s) 303
BbvI GCAGC 2 cut(s) 93, 102
BccI CCATC 1 cut(s) 307
BceAI ACGGC 1 cut(s) 215
BciT130I CCWGG 1 cut(s) 255
BcoDI GTCTC 1 cut(s) 23
BfaI CTAG 2 cut(s) 357, 418
BfmI CTRYAG 2 cut(s) 88, 334
BfoI RGCGCY 1 cut(s) 342
BglI GCCNNNNNGGC 1 cut(s) 358
BglII AGATCT 1 cut(s) 262
BisI GCNGC 2 cut(s) 82, 91
BlpI GCTNAGC 1 cut(s) 348
BlsI GCNGC 2 cut(s) 83, 92
Bme1390I CCNGG 1 cut(s) 255
BmiI GGNNCC 1 cut(s) 362
BmrFI CCNGG 1 cut(s) 255
BpiI GAAGAC 1 cut(s) 303
BpmI CTGGAG 1 cut(s) 397
Bpu1102I GCTNAGC 1 cut(s) 348
BsaHI GRCGYC 1 cut(s) 228
Bsc4I CCNNNNNNNGG 1 cut(s) 313
BseBI CCWGG 1 cut(s) 255
BseLI CCNNNNNNNGG 1 cut(s) 313
BseRI GAGGAG 1 cut(s) 193
BseXI GCAGC 2 cut(s) 93, 102
BsgI GTGCAG 1 cut(s) 100
Bsh1285I CGRYCG 1 cut(s) 328
BsiEI CGRYCG 1 cut(s) 328
BslI CCNNNNNNNGG 1 cut(s) 313
BsmAI GTCTC 1 cut(s) 23
Bsp143I GATC 1 cut(s) 262
Bsp1720I GCTNAGC 1 cut(s) 348
BspACI CCGC 2 cut(s) 138, 345
BspLI GGNNCC 1 cut(s) 362
BspMAI CTGCAG 1 cut(s) 92
BssMI GATC 1 cut(s) 262
BssNI GRCGYC 1 cut(s) 228
Bst2UI CCWGG 1 cut(s) 255
Bst4CI ACNGT 1 cut(s) 326
BstACI GRCGYC 1 cut(s) 228
BstAPI GCANNNNNTGC 1 cut(s) 87
BstC8I GCNNGC 1 cut(s) 126
BstDEI CTNAG 2 cut(s) 195, 348
BstH2I RGCGCY 1 cut(s) 342
BstHHI GCGC 1 cut(s) 341
BstKTI GATC 1 cut(s) 265
BstMAI GTCTC 1 cut(s) 23
BstMBI GATC 1 cut(s) 262
BstMCI CGRYCG 1 cut(s) 328
BstMWI GCNNNNNNNGC 3 cut(s) 87, 90, 358
BstNI CCWGG 1 cut(s) 255
BstSCI CCNGG 1 cut(s) 253
BstSFI CTRYAG 2 cut(s) 88, 334
BstV1I GCAGC 2 cut(s) 93, 102
BstV2I GAAGAC 1 cut(s) 303
BstX2I RGATCY 1 cut(s) 262
BstXI CCANNNNNNTGG 1 cut(s) 254
BstYI RGATCY 1 cut(s) 262
Cac8I GCNNGC 1 cut(s) 126
CaiI CAGNNNCTG 1 cut(s) 87
CfoI GCGC 1 cut(s) 341
CseI GACGC 1 cut(s) 236
Csp6I GTAC 2 cut(s) 244, 322
CviAII CATG 3 cut(s) 76, 113, 310
CviQI GTAC 2 cut(s) 244, 322
DdeI CTNAG 2 cut(s) 195, 348
DpnI GATC 1 cut(s) 264
DpnII GATC 1 cut(s) 262
DraIII CACNNNGTG 1 cut(s) 399
Eco47III AGCGCT 1 cut(s) 340
Eco57I CTGAAG 1 cut(s) 391
EcoRII CCWGG 1 cut(s) 253
FaeI CATG 3 cut(s) 79, 116, 313
FaiI YATR 4 cut(s) 77, 114, 278, 311
FatI CATG 3 cut(s) 75, 112, 309
FblI GTMKAC 1 cut(s) 328
Fnu4HI GCNGC 2 cut(s) 82, 91
Fsp4HI GCNGC 2 cut(s) 82, 91
FspBI CTAG 2 cut(s) 357, 418
GlaI GCGC 1 cut(s) 340
GluI GCNGC 2 cut(s) 82, 91
GsuI CTGGAG 1 cut(s) 397
HaeII RGCGCY 1 cut(s) 342
HgaI GACGC 1 cut(s) 236
HhaI GCGC 1 cut(s) 341
Hin1I GRCGYC 1 cut(s) 228
Hin1II CATG 3 cut(s) 79, 116, 313
Hin6I GCGC 1 cut(s) 339
HinP1I GCGC 1 cut(s) 339
HincII GTYRAC 1 cut(s) 329
HindII GTYRAC 1 cut(s) 329
HindIII AAGCTT 1 cut(s) 176
HinfI GANTC 1 cut(s) 330
HphI GGTGA 2 cut(s) 152, 277
Hpy166II GTNNAC 1 cut(s) 329
Hpy188III TCNNGA 3 cut(s) 26, 266, 383
Hpy8I GTNNAC 1 cut(s) 329
Hpy99I CGWCG 1 cut(s) 296
HpyCH4III ACNGT 1 cut(s) 326
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 2 cut(s) 81, 90
HpyF10VI GCNNNNNNNGC 3 cut(s) 87, 90, 358
HpyF3I CTNAG 2 cut(s) 195, 348
HpySE526I ACGT 1 cut(s) 132
Hsp92I GRCGYC 1 cut(s) 228
Hsp92II CATG 3 cut(s) 79, 116, 313
HspAI GCGC 1 cut(s) 339
Kzo9I GATC 1 cut(s) 262
LmnI GCTCC 2 cut(s) 366, 416
LpnPI CCDG 5 cut(s) 113, 134, 240, 260, 267
Lsp1109I GCAGC 2 cut(s) 93, 102
MaeI CTAG 2 cut(s) 357, 418
MaeII ACGT 1 cut(s) 132
MaeIII GTNAC 3 cut(s) 140, 212, 283
MalI GATC 1 cut(s) 264
MboI GATC 1 cut(s) 262
MboII GAAGA 1 cut(s) 308
MflI RGATCY 1 cut(s) 262
MlyI GAGTC 1 cut(s) 324
MmeI TCCRAC 2 cut(s) 81, 388
MnlI CCTC 8 cut(s) 64, 114, 164, 214, 218, 229, 311, 390
MseI TTAA 2 cut(s) 69, 162
MspA1I CMGCKG 1 cut(s) 138
MspR9I CCNGG 1 cut(s) 255
MvaI CCWGG 1 cut(s) 255
MwoI GCNNNNNNNGC 3 cut(s) 87, 90, 358
NdeII GATC 1 cut(s) 262
NlaIII CATG 3 cut(s) 79, 116, 313
NlaIV GGNNCC 1 cut(s) 362
NmuCI GTSAC 3 cut(s) 140, 212, 283
PcsI WCGNNNNNNNCGW 1 cut(s) 300
PflMI CCANNNNNTGG 1 cut(s) 313
PkrI GCNGC 2 cut(s) 83, 92
PleI GAGTC 1 cut(s) 324
PpsI GAGTC 1 cut(s) 324
Psp6I CCWGG 1 cut(s) 253
PspGI CCWGG 1 cut(s) 253
PspN4I GGNNCC 1 cut(s) 362
PstI CTGCAG 1 cut(s) 92
PstNI CAGNNNCTG 1 cut(s) 87
PsuI RGATCY 1 cut(s) 262
RsaI GTAC 2 cut(s) 245, 323
RsaNI GTAC 2 cut(s) 244, 322
SalI GTCGAC 1 cut(s) 327
SaqAI TTAA 2 cut(s) 69, 162
SatI GCNGC 2 cut(s) 82, 91
Sau3AI GATC 1 cut(s) 262
SchI GAGTC 1 cut(s) 324
ScrFI CCNGG 1 cut(s) 255
SfcI CTRYAG 2 cut(s) 88, 334
SsiI CCGC 2 cut(s) 138, 345
SspMI CTAG 2 cut(s) 357, 418
StyD4I CCNGG 1 cut(s) 253
TaaI ACNGT 1 cut(s) 326
TaiI ACGT 1 cut(s) 135
TaqI TCGA 4 cut(s) 8, 303, 318, 328
Tru1I TTAA 2 cut(s) 69, 162
Tru9I TTAA 2 cut(s) 69, 162
TseFI GTSAC 3 cut(s) 140, 212, 283
TseI GCWGC 2 cut(s) 81, 90
Tsp45I GTSAC 3 cut(s) 140, 212, 283
TspDTI ATGAA 3 cut(s) 17, 64, 178
Van91I CCANNNNNTGG 1 cut(s) 313
XmiI GTMKAC 1 cut(s) 328
XspI CTAG 2 cut(s) 357, 418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.