RchiOBHm_Chr7g0233221

PB1 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0002525
Physical Location & Seq
Forward (+)
57659465 .. 57662536
3072 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20903

Sequence Viewer

Length: 1443 bp
ATGGAGAACTACTCCTACCCGGACTCCCGCGAGTCGTCCCCACGCTCCCGCGAAATCGACTGCGACACTCCCTCCTGGGACGATCAGCCTCCCTCCTCCGCCGCCGCCGCCGCCACCTACAAGGTCAAGCTCATGTGCAGCTACGGCGGCAAAATCCAGCCCCGGCCCCACGACAACCAGCTCGCCTACGTCGGCGGCGACACCAAAATCCTCGCCGTCGATCGCAGCATCAAGCTCTCCGCCTTCATTTCCAAGCTCTCCTCCCTCTGCGAAACCCCCGACGGCGCCGTCTGCTTCAAGTACCAGCTCCCCGGCGAGGATCTCGACGCCCTCATCTCCGTCACCAACGACGAGGACCTCGACCACATGATGATCGAGTACGACCGCCTCTACCGCGCCTCCGCTAAGCCCGCGCGTCTCAGACTCTTCCTCTTCCCGATCAACCCTTCCGCTCCGGCCAGCTTCGGCTCCAACGAGTCCAAGTCCGAGCGCCAGTGGTTCGTCGACGCCTTGAATTCCGTTCAGATCCAGTCCCCGGAAGGCTCCTCTCCCACCGCTCCGGTCTCCGCCTCCTCCACCAACCCCGATTTCCTCTTCGGATTCGACAAGGGATACGCCGCCGCCGCCGCTCCCGTCGCGAAATTGCCTGATTCCGTCACGCCTCCGACTGTTCCTGATTCGCTTGTGAAGAACACGCTCGCCGCCGGACCGGAATTGGTCTCGGAGCCGACGCTGTCTCAATTCGAGATTCAGAGGCAGATTCAGGAGCTACACAGAATGCAAATTGCTGCCGGTCACGAGCCGACTATCTATCAGAGGAAAAACGAAGAGGTTCCGAACCCTCGGGCTTACACAGCTGATTATTACTCTCAGAAGCTTCCAGAAAACATAGCTGCTCCGGCGCCACCGGGAGCCGCTCCTGTTCCAATGCCGGCACACATGCAATTTCCGACTTCTTATATGCCGGAAAGGCACATAAACACTGGAAGTTATCAGGTGGCGGCGGCTCCCGGTGGAGGCGAGCAGCCTGTGTACCTCATTTCCACACCAGCTGGTGTATACCAGGCTCCGGCTCATAGACCGGCCACTGGACCAGTCGGGCAGGCTTACTATGGTGTACCGCAGAGAATGGTTCCCGAGGTGTACCGTGACGCACAGGCATACAATACGGCAGTGGCAGCTCAGCAGCAGCAGCAGCAACAACAACAGCAGCAAGCAAAGGTGGCTGCTTACAGTGAAGGGTTTACACAAGTGGGTTATGACAATTCTGGGAGGCAAGTGTACTACACTACAGCCGGAGGCGCGGTGGCTTCATCTTACCAGACTGTGGCGCCAGTACCGGTTGCTGTTGATGCAAGACAAGGTGGTGGTGGCGCTTTGAATCAAATTCAAGACGGTAAGGTGCTAGGTGAAGAGCAGGAGGGCTACAGTAGGAAACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

480

Amino Acids

51.88

Weight (kDa)

5.16

Isoelectric Point (pI)

51.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 57 - 146 6.4e-16 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 287
AccB1I GGYRCC 3 cut(s) 284, 901, 1330
AccB7I CCANNNNNTGG 1 cut(s) 1327
AccBSI CCGCTC 4 cut(s) 452, 557, 629, 917
AccI GTMKAC 2 cut(s) 504, 1059
AccII CGCG 7 cut(s) 30, 51, 396, 413, 415, 638, 1304
AclWI GGATC 2 cut(s) 327, 520
AcoI YGGCCR 2 cut(s) 456, 1083
AcsI RAATTY 2 cut(s) 514, 1386
AcyI GRCGYC 5 cut(s) 285, 327, 507, 902, 1331
AfaI GTAC 7 cut(s) 302, 380, 1034, 1119, 1145, 1283, 1338
AfiI CCNNNNNNNGG 6 cut(s) 316, 535, 1016, 1069, 1088, 1327
AgeI ACCGGT 1 cut(s) 1339
AgsI TTSAA 4 cut(s) 298, 514, 1381, 1391
AjnI CCWGG 2 cut(s) 74, 1062
AjuI GAANNNNNNNTTGG 2 cut(s) 572, 604
Alw26I GTCTC 4 cut(s) 422, 568, 724, 741
AlwI GGATC 2 cut(s) 327, 520
Ama87I CYCGRG 2 cut(s) 843, 1136
AoxI GGCC 3 cut(s) 164, 456, 1083
ApoI RAATTY 2 cut(s) 514, 1386
AsiGI ACCGGT 1 cut(s) 1339
Asp700I GAANNNNTTC 1 cut(s) 831
AspLEI GCGC 8 cut(s) 287, 398, 415, 492, 904, 1304, 1333, 1376
AspS9I GGNCC 4 cut(s) 165, 355, 707, 1091
AsuC2I CCSGG 6 cut(s) 20, 163, 312, 536, 909, 1011
AsuHPI GGTGA 2 cut(s) 334, 1421
AvaI CYCGRG 2 cut(s) 843, 1136
AvaII GGWCC 3 cut(s) 355, 707, 1091
BanI GGYRCC 3 cut(s) 284, 901, 1330
BauI CACGAG 1 cut(s) 797
BceAI ACGGC 5 cut(s) 160, 200, 272, 298, 1185
BciT130I CCWGG 2 cut(s) 76, 1064
BciVI GTATCC 1 cut(s) 605
BcnI CCSGG 6 cut(s) 20, 163, 312, 536, 909, 1011
BcoDI GTCTC 4 cut(s) 422, 568, 724, 741
BfaI CTAG 1 cut(s) 1406
BfmI CTRYAG 2 cut(s) 1290, 1426
BfoI RGCGCY 5 cut(s) 288, 493, 905, 1334, 1377
BfuI GTATCC 1 cut(s) 605
BglI GCCNNNNNGGC 1 cut(s) 970
BlpI GCTNAGC 2 cut(s) 405, 1182
Bme1390I CCNGG 8 cut(s) 20, 76, 163, 312, 536, 909, 1011, 1064
Bme18I GGWCC 3 cut(s) 355, 707, 1091
BmeT110I CYCGRG 2 cut(s) 843, 1136
BmgT120I GGNCC 4 cut(s) 165, 355, 707, 1091
BmrFI CCNGG 8 cut(s) 20, 76, 163, 312, 536, 909, 1011, 1064
BmsI GCATC 2 cut(s) 237, 1342
BplI GAGNNNNNCTC 1 cut(s) 28
Bpu1102I GCTNAGC 2 cut(s) 405, 1182
BpuMI CCSGG 6 cut(s) 20, 163, 312, 536, 909, 1011
BsaHI GRCGYC 5 cut(s) 285, 327, 507, 902, 1331
BsaI GGTCTC 2 cut(s) 568, 724
BsaJI CCNNGG 6 cut(s) 75, 161, 310, 534, 842, 1137
BsaWI WCCGGW 3 cut(s) 559, 709, 1339
BsaXI ACNNNNNCTCC 8 cut(s) 8, 38, 56, 86, 383, 413, 1290, 1320
Bsc4I CCNNNNNNNGG 6 cut(s) 316, 535, 1016, 1069, 1088, 1327
Bse118I RCCGGY 4 cut(s) 791, 931, 1081, 1339
Bse1I ACTGG 6 cut(s) 493, 529, 988, 1093, 1094, 1334
BseBI CCWGG 2 cut(s) 76, 1064
BseDI CCNNGG 6 cut(s) 75, 161, 310, 534, 842, 1137
BseLI CCNNNNNNNGG 6 cut(s) 316, 535, 1016, 1069, 1088, 1327
BseMII CTCAG 3 cut(s) 433, 884, 1196
BseNI ACTGG 6 cut(s) 493, 529, 988, 1093, 1094, 1334
BseRI GAGGAG 4 cut(s) 85, 250, 535, 562
BsgI GTGCAG 1 cut(s) 157
Bsh1236I CGCG 7 cut(s) 30, 51, 396, 413, 415, 638, 1304
Bsh1285I CGRYCG 2 cut(s) 223, 385
BshFI GGCC 3 cut(s) 166, 458, 1085
BshNI GGYRCC 3 cut(s) 284, 901, 1330
BshTI ACCGGT 1 cut(s) 1339
BsiEI CGRYCG 2 cut(s) 223, 385
BsiHKCI CYCGRG 2 cut(s) 843, 1136
BslFI GGGAC 3 cut(s) 22, 92, 517
BslI CCNNNNNNNGG 6 cut(s) 316, 535, 1016, 1069, 1088, 1327
BsmAI GTCTC 4 cut(s) 422, 568, 724, 741
BsmBI CGTCTC 1 cut(s) 422
BsmFI GGGAC 3 cut(s) 22, 92, 517
BsmI GAATGC 1 cut(s) 783
BsnI GGCC 3 cut(s) 166, 458, 1085
Bso31I GGTCTC 2 cut(s) 568, 724
BsoBI CYCGRG 2 cut(s) 843, 1136
Bsp143I GATC 6 cut(s) 82, 220, 319, 372, 438, 525
Bsp1720I GCTNAGC 2 cut(s) 405, 1182
Bsp68I TCGCGA 1 cut(s) 638
BspANI GGCC 3 cut(s) 166, 458, 1085
BspCNI CTCAG 3 cut(s) 432, 883, 1195
BspFNI CGCG 7 cut(s) 30, 51, 396, 413, 415, 638, 1304
BspPI GGATC 2 cut(s) 327, 520
BspQI GCTCTTC 1 cut(s) 1407
BspT107I GGYRCC 3 cut(s) 284, 901, 1330
BspTNI GGTCTC 2 cut(s) 568, 724
BsrBI CCGCTC 4 cut(s) 452, 557, 629, 917
BsrFI RCCGGY 4 cut(s) 791, 931, 1081, 1339
BsrI ACTGG 6 cut(s) 493, 529, 988, 1093, 1094, 1334
BssAI RCCGGY 4 cut(s) 791, 931, 1081, 1339
BssECI CCNNGG 6 cut(s) 75, 161, 310, 534, 842, 1137
BssMI GATC 6 cut(s) 82, 220, 319, 372, 438, 525
BssNAI GTATAC 1 cut(s) 1060
BssNI GRCGYC 5 cut(s) 285, 327, 507, 902, 1331
BssSI CACGAG 1 cut(s) 797
Bst1107I GTATAC 1 cut(s) 1060
Bst2BI CACGAG 1 cut(s) 797
Bst2UI CCWGG 2 cut(s) 76, 1064
Bst4CI ACNGT 7 cut(s) 670, 1148, 1235, 1327, 1397, 1430, 1440
Bst6I CTCTTC 5 cut(s) 431, 437, 599, 822, 1407
BstACI GRCGYC 5 cut(s) 285, 327, 507, 902, 1331
BstC8I GCNNGC 8 cut(s) 183, 411, 460, 699, 933, 1022, 1104, 1215
BstDEI CTNAG 4 cut(s) 405, 419, 870, 1182
BstFNI CGCG 7 cut(s) 30, 51, 396, 413, 415, 638, 1304
BstH2I RGCGCY 5 cut(s) 288, 493, 905, 1334, 1377
BstHHI GCGC 8 cut(s) 287, 398, 415, 492, 904, 1304, 1333, 1376
BstKTI GATC 6 cut(s) 85, 223, 322, 375, 441, 528
BstMAI GTCTC 4 cut(s) 422, 568, 724, 741
BstMBI GATC 6 cut(s) 82, 220, 319, 372, 438, 525
BstMCI CGRYCG 2 cut(s) 223, 385
BstNI CCWGG 2 cut(s) 76, 1064
BstNSI RCATGY 1 cut(s) 943
BstSCI CCNGG 8 cut(s) 18, 74, 161, 310, 534, 907, 1009, 1062
BstSFI CTRYAG 2 cut(s) 1290, 1426
BstUI CGCG 7 cut(s) 30, 51, 396, 413, 415, 638, 1304
BstX2I RGATCY 2 cut(s) 319, 525
BstYI RGATCY 2 cut(s) 319, 525
BstZ17I GTATAC 1 cut(s) 1060
BsuI GTATCC 1 cut(s) 605
BsuRI GGCC 3 cut(s) 166, 458, 1085
BtsI GCAGTG 1 cut(s) 1179
BtsIMutI CAGTG 5 cut(s) 500, 981, 1086, 1179, 1240
BtuMI TCGCGA 1 cut(s) 638
Cac8I GCNNGC 8 cut(s) 183, 411, 460, 699, 933, 1022, 1104, 1215
CfoI GCGC 8 cut(s) 287, 398, 415, 492, 904, 1304, 1333, 1376
Cfr10I RCCGGY 4 cut(s) 791, 931, 1081, 1339
Cfr13I GGNCC 4 cut(s) 165, 355, 707, 1091
CpoI CGGWCCG 1 cut(s) 707
CseI GACGC 5 cut(s) 335, 404, 515, 739, 1160
Csp6I GTAC 7 cut(s) 301, 379, 1033, 1118, 1144, 1282, 1337
CspAI ACCGGT 1 cut(s) 1339
CspI CGGWCCG 1 cut(s) 707
CviAII CATG 3 cut(s) 133, 367, 940
CviQI GTAC 7 cut(s) 301, 379, 1033, 1118, 1144, 1282, 1337
DdeI CTNAG 4 cut(s) 405, 419, 870, 1182
DinI GGCGCC 3 cut(s) 286, 903, 1332
DpnI GATC 6 cut(s) 84, 222, 321, 374, 440, 527
DpnII GATC 6 cut(s) 82, 220, 319, 372, 438, 525
DrdI GACNNNNNNGTC 1 cut(s) 287
DseDI GACNNNNNNGTC 1 cut(s) 287
EaeI YGGCCR 2 cut(s) 456, 1083
Eam1104I CTCTTC 5 cut(s) 431, 437, 599, 822, 1407
EarI CTCTTC 5 cut(s) 431, 437, 599, 822, 1407
EciI GGCGGA 3 cut(s) 88, 229, 556
Eco31I GGTCTC 2 cut(s) 568, 724
Eco47I GGWCC 3 cut(s) 355, 707, 1091
Eco88I CYCGRG 2 cut(s) 843, 1136
EcoO109I RGGNCCY 1 cut(s) 355
EcoRI GAATTC 1 cut(s) 514
EcoRII CCWGG 2 cut(s) 74, 1062
EgeI GGCGCC 3 cut(s) 286, 903, 1332
EheI GGCGCC 3 cut(s) 286, 903, 1332
Esp3I CGTCTC 1 cut(s) 422
FaeI CATG 3 cut(s) 136, 370, 943
FaqI GGGAC 3 cut(s) 22, 92, 517
FatI CATG 3 cut(s) 132, 366, 939
FauI CCCGC 3 cut(s) 35, 56, 418
FblI GTMKAC 2 cut(s) 504, 1059
FspBI CTAG 1 cut(s) 1406
GlaI GCGC 8 cut(s) 286, 397, 414, 491, 903, 1303, 1332, 1375
HaeII RGCGCY 5 cut(s) 288, 493, 905, 1334, 1377
HaeIII GGCC 3 cut(s) 166, 458, 1085
HgaI GACGC 5 cut(s) 335, 404, 515, 739, 1160
HhaI GCGC 8 cut(s) 287, 398, 415, 492, 904, 1304, 1333, 1376
Hin1I GRCGYC 5 cut(s) 285, 327, 507, 902, 1331
Hin1II CATG 3 cut(s) 136, 370, 943
Hin6I GCGC 8 cut(s) 285, 396, 413, 490, 902, 1302, 1331, 1374
HinP1I GCGC 8 cut(s) 285, 396, 413, 490, 902, 1302, 1331, 1374
HincII GTYRAC 1 cut(s) 505
HindII GTYRAC 1 cut(s) 505
HindIII AAGCTT 1 cut(s) 875
HphI GGTGA 2 cut(s) 334, 1421
Hpy166II GTNNAC 7 cut(s) 505, 1033, 1060, 1118, 1144, 1245, 1282
Hpy8I GTNNAC 7 cut(s) 505, 1033, 1060, 1118, 1144, 1245, 1282
Hpy99I CGWCG 9 cut(s) 194, 221, 284, 329, 353, 506, 509, 638, 733
HpyAV CCTTC 4 cut(s) 253, 456, 533, 1232
HpyCH4III ACNGT 7 cut(s) 670, 1148, 1235, 1327, 1397, 1430, 1440
HpyCH4IV ACGT 1 cut(s) 189
HpyCH4V TGCA 4 cut(s) 138, 781, 943, 1355
HpyF3I CTNAG 4 cut(s) 405, 419, 870, 1182
HpySE526I ACGT 1 cut(s) 189
Hsp92I GRCGYC 5 cut(s) 285, 327, 507, 902, 1331
Hsp92II CATG 3 cut(s) 136, 370, 943
HspAI GCGC 8 cut(s) 285, 396, 413, 490, 902, 1302, 1331, 1374
KasI GGCGCC 3 cut(s) 284, 901, 1330
KroI GCCGGC 1 cut(s) 931
KroNI GCCGGC 1 cut(s) 933
Kzo9I GATC 6 cut(s) 82, 220, 319, 372, 438, 525
LguI GCTCTTC 1 cut(s) 1407
LweI GCATC 2 cut(s) 237, 1342
MaeI CTAG 1 cut(s) 1406
MaeII ACGT 1 cut(s) 189
MaeIII GTNAC 4 cut(s) 340, 655, 794, 1148
MalI GATC 6 cut(s) 84, 222, 321, 374, 440, 527
MbiI CCGCTC 4 cut(s) 452, 557, 629, 917
MboI GATC 6 cut(s) 82, 220, 319, 372, 438, 525
MboII GAAGA 6 cut(s) 418, 424, 586, 700, 839, 1424
MflI RGATCY 2 cut(s) 319, 525
MluCI AATT 8 cut(s) 514, 641, 713, 740, 783, 944, 1264, 1386
Mly113I GGCGCC 3 cut(s) 285, 902, 1331
MlyI GAGTC 4 cut(s) 17, 41, 417, 485
MmeI TCCRAC 3 cut(s) 495, 689, 974
MroNI GCCGGC 1 cut(s) 931
MroXI GAANNNNTTC 1 cut(s) 831
MspA1I CMGCKG 2 cut(s) 857, 1052
MspR9I CCNGG 8 cut(s) 20, 76, 163, 312, 536, 909, 1011, 1064
Mva1269I GAATGC 1 cut(s) 783
MvaI CCWGG 2 cut(s) 76, 1064
MvnI CGCG 7 cut(s) 30, 51, 396, 413, 415, 638, 1304
NaeI GCCGGC 1 cut(s) 933
NarI GGCGCC 3 cut(s) 285, 902, 1331
NciI CCSGG 6 cut(s) 20, 163, 312, 536, 909, 1011
NdeII GATC 6 cut(s) 82, 220, 319, 372, 438, 525
NgoMIV GCCGGC 1 cut(s) 931
NlaIII CATG 3 cut(s) 136, 370, 943
NmuCI GTSAC 4 cut(s) 340, 655, 794, 1148
NruI TCGCGA 1 cut(s) 638
NspI RCATGY 1 cut(s) 943
PciSI GCTCTTC 1 cut(s) 1407
PcsI WCGNNNNNNNCGW 3 cut(s) 195, 357, 471
PctI GAATGC 1 cut(s) 783
PdiI GCCGGC 1 cut(s) 933
PdmI GAANNNNTTC 1 cut(s) 831
PfeI GAWTC 6 cut(s) 600, 650, 677, 748, 760, 1381
PflFI GACNNNGTC 1 cut(s) 733
PflMI CCANNNNNTGG 1 cut(s) 1327
PinAI ACCGGT 1 cut(s) 1339
Ple19I CGATCG 1 cut(s) 223
PleI GAGTC 4 cut(s) 17, 40, 417, 484
PluTI GGCGCC 3 cut(s) 288, 905, 1334
PpsI GAGTC 4 cut(s) 17, 40, 417, 484
PpuMI RGGWCCY 1 cut(s) 355
Psp5II RGGWCCY 1 cut(s) 355
Psp6I CCWGG 2 cut(s) 74, 1062
PspGI CCWGG 2 cut(s) 74, 1062
PspPI GGNCC 4 cut(s) 165, 355, 707, 1091
PspPPI RGGWCCY 1 cut(s) 355
PsrI GAACNNNNNNTAC 1 cut(s) 31
PsuI RGATCY 2 cut(s) 319, 525
PsyI GACNNNGTC 1 cut(s) 733
PvuI CGATCG 1 cut(s) 223
PvuII CAGCTG 2 cut(s) 857, 1052
RruI TCGCGA 1 cut(s) 638
RsaI GTAC 7 cut(s) 302, 380, 1034, 1119, 1145, 1283, 1338
RsaNI GTAC 7 cut(s) 301, 379, 1033, 1118, 1144, 1282, 1337
Rsr2I CGGWCCG 1 cut(s) 707
RsrII CGGWCCG 1 cut(s) 707
SalI GTCGAC 1 cut(s) 503
SapI GCTCTTC 1 cut(s) 1407
Sau3AI GATC 6 cut(s) 82, 220, 319, 372, 438, 525
Sau96I GGNCC 4 cut(s) 165, 355, 707, 1091
SchI GAGTC 4 cut(s) 17, 41, 417, 485
ScrFI CCNGG 8 cut(s) 20, 76, 163, 312, 536, 909, 1011, 1064
SfaNI GCATC 2 cut(s) 237, 1342
SfcI CTRYAG 2 cut(s) 1290, 1426
SfoI GGCGCC 3 cut(s) 286, 903, 1332
SgrDI CGTCGACG 1 cut(s) 503
SinI GGWCC 3 cut(s) 355, 707, 1091
Sse9I AATT 8 cut(s) 514, 641, 713, 740, 783, 944, 1264, 1386
SspDI GGCGCC 3 cut(s) 284, 901, 1330
SspMI CTAG 1 cut(s) 1406
StyD4I CCNGG 8 cut(s) 18, 74, 161, 310, 534, 907, 1009, 1062
TaaI ACNGT 7 cut(s) 670, 1148, 1235, 1327, 1397, 1430, 1440
TaiI ACGT 1 cut(s) 192
TaqI TCGA 8 cut(s) 57, 219, 324, 360, 375, 504, 603, 744
TasI AATT 8 cut(s) 514, 641, 713, 740, 783, 944, 1264, 1386
TatI WGTACW 1 cut(s) 1281
TfiI GAWTC 6 cut(s) 600, 650, 677, 748, 760, 1381
TscAI CASTG 5 cut(s) 500, 988, 1093, 1179, 1240
TseFI GTSAC 4 cut(s) 340, 655, 794, 1148
Tsp45I GTSAC 4 cut(s) 340, 655, 794, 1148
TspDTI ATGAA 2 cut(s) 235, 1302
TspGWI ACGGA 3 cut(s) 328, 508, 643
TspRI CASTG 5 cut(s) 500, 988, 1093, 1179, 1240
Tth111I GACNNNGTC 1 cut(s) 733
Van91I CCANNNNNTGG 1 cut(s) 1327
VpaK11BI GGWCC 3 cut(s) 355, 707, 1091
XapI RAATTY 2 cut(s) 514, 1386
XceI RCATGY 1 cut(s) 943
XmiI GTMKAC 2 cut(s) 504, 1059
XmnI GAANNNNTTC 1 cut(s) 831
XspI CTAG 1 cut(s) 1406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.