Rw7G035810

PB1 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0002525
Physical Location & Seq
Forward (+)
53806153 .. 53808158
2006 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G035810.1

Sequence Viewer

Length: 1461 bp
ATGGAGAACTACTCCTACCCGGACTCCCGCGAGTCGTCCCCACGCTCCCGCGAAATCGACTGCGACACTCCCTCCTGGGACGATCAGCCTCCCTCCTCCGCCGCCGCCGCCTCCGCCGCCACCACCTACAAGGTCAAGCTCATGTGCAGCTACGGCGGCAAAATCCAGCCCCGGCCCCACGACAACCAGCTCGCCTACGTCGGCGGCGACACCAAAATCCTCGCCGTCGATCGCAGCATCAAGCTCTCCGCCTTCATTTCCAAGCTCTCCTCCCTCTGCGAAACCCCCGACGGCGCCGTCTGCTTCAAGTACCAGCTCCCCGGCGAGGATCTCGACGCCCTCATCTCCGTCACCAACGACGAGGACCTCGACCACATGATGATCGAGTACGACCGCCTCTACCGCGCCTCCGCTAAGCCCGCGCGTCTCAGGCTCTTCCTCTTCCCGATCAACCCTTCCGCTCCGGCCAGCTTCGGCTCCAACGAGTCCAAGTCCGAGCGCCAGTGGTTCGTCGACGCCTTGAATTCCGTTCAGATCCAGTCCCCGGAAGGCTCCTCTCCCACCGCGCCGGTCTCCGCCTCCTCCACCAACCCCGATTTCCTCTTCGGATTCGACAAGGGATACGCCGCCGCCGCCGCCGCTCCCGTCGCGAAATTGCCTGATTCCGTCGCGCCTCCGACTGTTCCTGATTCGCTTGTGAAGAACACGCTCGCCGCCGGACCGGAATTGGTCTCGGAGCCGACGCTGTCCCAATTCGAGATTCAGAGGCAGATTCAGGAGCTACACAGAATGCAAATTGCCGCCGGTCACGAGCCGACTATCTATCAGAGGAAAAACGATGAGGTTCCGAACCCTCGGGCTTACACAGCTGATTATTACTCTCAGAAGCTTCCAGAAAACATAGCTGCTCCGGCGCCACCGGGAGCCGCTCCTGTTCCAATGCCGGCACACATGCAATTTCCGACTTCTTATATGCCGGAAAGGCACATAAACACTGGAAGTTATCAGGTGGCGGCGGCTCCCGGTGGAGGCGAGCAGCCTGTGTACCTCATTTCCACACCAGCTGGTGTATACCAGGCTCCGGCTCATAGACCGGCCACTGGACCAGTCGGGCAGGCTTACTATGGTGTGCCGCAGAGAATGGTTCCCGAGGTGTACCGTGACGCACAGGCATACAATACGGCAGTGGCAGCTCAGCAGCAGCAGCAACAACAGCAGCAGCAGCAGCAGCAAGCAAAGGTGGCTGCTTACAGTGAAGGGTTTACACAAGTGGGTTATGACAATTCCGGGAGGCAAGTGTACTACACTACAGCCGGAGGCGCGGTGGCTTCATCTTACCAGACTGTGGCGCCAGTACCGGTTGCTGTTGATGCAAGACAAGGTGGTGGTGGTGGCGCTTTGAATCAAATTCAAGACGGTAAGGTTGTTGCCTCCATGGCTGCCCAAGCCTCTGCTATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

486

Amino Acids

52.09

Weight (kDa)

5.19

Isoelectric Point (pI)

50.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 60 - 149 6.5e-16 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 296
AccB1I GGYRCC 3 cut(s) 293, 913, 1348
AccB7I CCANNNNNTGG 1 cut(s) 1345
AccBSI CCGCTC 3 cut(s) 461, 641, 929
AccI GTMKAC 2 cut(s) 513, 1071
AccII CGCG 9 cut(s) 30, 51, 405, 422, 424, 566, 650, 671, 1322
AclWI GGATC 2 cut(s) 336, 529
AcoI YGGCCR 2 cut(s) 465, 1095
AcsI RAATTY 2 cut(s) 523, 1407
AcyI GRCGYC 5 cut(s) 294, 336, 516, 914, 1349
AfaI GTAC 6 cut(s) 311, 389, 1046, 1157, 1301, 1356
AfiI CCNNNNNNNGG 6 cut(s) 325, 544, 1028, 1081, 1100, 1345
AgeI ACCGGT 1 cut(s) 1357
AgsI TTSAA 4 cut(s) 307, 523, 1402, 1412
AjnI CCWGG 2 cut(s) 74, 1074
AjuI GAANNNNNNNTTGG 4 cut(s) 581, 613, 744, 776
Alw26I GTCTC 3 cut(s) 431, 577, 736
AlwI GGATC 2 cut(s) 336, 529
Ama87I CYCGRG 2 cut(s) 855, 1148
AoxI GGCC 3 cut(s) 173, 465, 1095
ApoI RAATTY 2 cut(s) 523, 1407
AsiGI ACCGGT 1 cut(s) 1357
AspS9I GGNCC 4 cut(s) 174, 364, 719, 1103
AsuC2I CCSGG 7 cut(s) 20, 172, 321, 545, 921, 1023, 1288
AsuHPI GGTGA 1 cut(s) 343
AvaI CYCGRG 2 cut(s) 855, 1148
AvaII GGWCC 3 cut(s) 364, 719, 1103
BanI GGYRCC 3 cut(s) 293, 913, 1348
BauI CACGAG 1 cut(s) 809
BceAI ACGGC 5 cut(s) 169, 209, 281, 307, 1197
BciT130I CCWGG 2 cut(s) 76, 1076
BciVI GTATCC 1 cut(s) 614
BcnI CCSGG 7 cut(s) 20, 172, 321, 545, 921, 1023, 1288
BcoDI GTCTC 3 cut(s) 431, 577, 736
BfmI CTRYAG 1 cut(s) 1308
BfoI RGCGCY 5 cut(s) 297, 502, 917, 1352, 1398
BfuI GTATCC 1 cut(s) 614
BglI GCCNNNNNGGC 2 cut(s) 982, 1436
BlpI GCTNAGC 2 cut(s) 414, 1194
Bme1390I CCNGG 9 cut(s) 20, 76, 172, 321, 545, 921, 1023, 1076, 1288
Bme18I GGWCC 3 cut(s) 364, 719, 1103
BmeT110I CYCGRG 2 cut(s) 855, 1148
BmgT120I GGNCC 4 cut(s) 174, 364, 719, 1103
BmrFI CCNGG 9 cut(s) 20, 76, 172, 321, 545, 921, 1023, 1076, 1288
BmsI GCATC 2 cut(s) 246, 1360
BplI GAGNNNNNCTC 1 cut(s) 28
Bpu1102I GCTNAGC 2 cut(s) 414, 1194
BpuMI CCSGG 7 cut(s) 20, 172, 321, 545, 921, 1023, 1288
BsaHI GRCGYC 5 cut(s) 294, 336, 516, 914, 1349
BsaI GGTCTC 2 cut(s) 577, 736
BsaJI CCNNGG 7 cut(s) 75, 170, 319, 543, 854, 1149, 1434
BsaWI WCCGGW 2 cut(s) 721, 1357
BsaXI ACNNNNNCTCC 8 cut(s) 8, 38, 56, 86, 392, 422, 1308, 1338
Bsc4I CCNNNNNNNGG 6 cut(s) 325, 544, 1028, 1081, 1100, 1345
Bse118I RCCGGY 5 cut(s) 568, 803, 943, 1093, 1357
Bse1I ACTGG 6 cut(s) 502, 538, 1000, 1105, 1106, 1352
BseBI CCWGG 2 cut(s) 76, 1076
BseDI CCNNGG 7 cut(s) 75, 170, 319, 543, 854, 1149, 1434
BseLI CCNNNNNNNGG 6 cut(s) 325, 544, 1028, 1081, 1100, 1345
BseMII CTCAG 3 cut(s) 442, 896, 1208
BseNI ACTGG 6 cut(s) 502, 538, 1000, 1105, 1106, 1352
BseRI GAGGAG 4 cut(s) 85, 259, 544, 571
BsgI GTGCAG 1 cut(s) 166
Bsh1236I CGCG 9 cut(s) 30, 51, 405, 422, 424, 566, 650, 671, 1322
Bsh1285I CGRYCG 2 cut(s) 232, 394
BshFI GGCC 3 cut(s) 175, 467, 1097
BshNI GGYRCC 3 cut(s) 293, 913, 1348
BshTI ACCGGT 1 cut(s) 1357
BsiEI CGRYCG 2 cut(s) 232, 394
BsiHKCI CYCGRG 2 cut(s) 855, 1148
BslFI GGGAC 4 cut(s) 22, 92, 526, 733
BslI CCNNNNNNNGG 6 cut(s) 325, 544, 1028, 1081, 1100, 1345
BsmAI GTCTC 3 cut(s) 431, 577, 736
BsmBI CGTCTC 1 cut(s) 431
BsmFI GGGAC 4 cut(s) 22, 92, 526, 733
BsmI GAATGC 1 cut(s) 795
BsnI GGCC 3 cut(s) 175, 467, 1097
Bso31I GGTCTC 2 cut(s) 577, 736
BsoBI CYCGRG 2 cut(s) 855, 1148
Bsp143I GATC 6 cut(s) 82, 229, 328, 381, 447, 534
Bsp1720I GCTNAGC 2 cut(s) 414, 1194
Bsp19I CCATGG 1 cut(s) 1434
Bsp68I TCGCGA 1 cut(s) 650
BspANI GGCC 3 cut(s) 175, 467, 1097
BspCNI CTCAG 3 cut(s) 441, 895, 1207
BspFNI CGCG 9 cut(s) 30, 51, 405, 422, 424, 566, 650, 671, 1322
BspPI GGATC 2 cut(s) 336, 529
BspQI GCTCTTC 1 cut(s) 440
BspT107I GGYRCC 3 cut(s) 293, 913, 1348
BspTNI GGTCTC 2 cut(s) 577, 736
BsrBI CCGCTC 3 cut(s) 461, 641, 929
BsrFI RCCGGY 5 cut(s) 568, 803, 943, 1093, 1357
BsrI ACTGG 6 cut(s) 502, 538, 1000, 1105, 1106, 1352
BssAI RCCGGY 5 cut(s) 568, 803, 943, 1093, 1357
BssECI CCNNGG 7 cut(s) 75, 170, 319, 543, 854, 1149, 1434
BssMI GATC 6 cut(s) 82, 229, 328, 381, 447, 534
BssNAI GTATAC 1 cut(s) 1072
BssNI GRCGYC 5 cut(s) 294, 336, 516, 914, 1349
BssSI CACGAG 1 cut(s) 809
BssT1I CCWWGG 1 cut(s) 1434
Bst1107I GTATAC 1 cut(s) 1072
Bst2BI CACGAG 1 cut(s) 809
Bst2UI CCWGG 2 cut(s) 76, 1076
Bst4CI ACNGT 5 cut(s) 682, 1160, 1253, 1345, 1418
Bst6I CTCTTC 3 cut(s) 440, 446, 608
BstACI GRCGYC 5 cut(s) 294, 336, 516, 914, 1349
BstC8I GCNNGC 8 cut(s) 192, 420, 469, 711, 945, 1034, 1116, 1233
BstDEI CTNAG 4 cut(s) 414, 428, 882, 1194
BstDSI CCRYGG 1 cut(s) 1434
BstFNI CGCG 9 cut(s) 30, 51, 405, 422, 424, 566, 650, 671, 1322
BstH2I RGCGCY 5 cut(s) 297, 502, 917, 1352, 1398
BstKTI GATC 6 cut(s) 85, 232, 331, 384, 450, 537
BstMAI GTCTC 3 cut(s) 431, 577, 736
BstMBI GATC 6 cut(s) 82, 229, 328, 381, 447, 534
BstMCI CGRYCG 2 cut(s) 232, 394
BstNI CCWGG 2 cut(s) 76, 1076
BstNSI RCATGY 1 cut(s) 955
BstSCI CCNGG 9 cut(s) 18, 74, 170, 319, 543, 919, 1021, 1074, 1286
BstSFI CTRYAG 1 cut(s) 1308
BstUI CGCG 9 cut(s) 30, 51, 405, 422, 424, 566, 650, 671, 1322
BstX2I RGATCY 2 cut(s) 328, 534
BstYI RGATCY 2 cut(s) 328, 534
BstZ17I GTATAC 1 cut(s) 1072
BsuI GTATCC 1 cut(s) 614
BsuRI GGCC 3 cut(s) 175, 467, 1097
BtgI CCRYGG 1 cut(s) 1434
BtsI GCAGTG 1 cut(s) 1191
BtsIMutI CAGTG 5 cut(s) 509, 993, 1098, 1191, 1258
BtuMI TCGCGA 1 cut(s) 650
Cac8I GCNNGC 8 cut(s) 192, 420, 469, 711, 945, 1034, 1116, 1233
Cfr10I RCCGGY 5 cut(s) 568, 803, 943, 1093, 1357
Cfr13I GGNCC 4 cut(s) 174, 364, 719, 1103
CpoI CGGWCCG 1 cut(s) 719
CseI GACGC 5 cut(s) 344, 413, 524, 751, 1172
Csp6I GTAC 6 cut(s) 310, 388, 1045, 1156, 1300, 1355
CspAI ACCGGT 1 cut(s) 1357
CspI CGGWCCG 1 cut(s) 719
CviAII CATG 4 cut(s) 142, 376, 952, 1435
CviQI GTAC 6 cut(s) 310, 388, 1045, 1156, 1300, 1355
DdeI CTNAG 4 cut(s) 414, 428, 882, 1194
DinI GGCGCC 3 cut(s) 295, 915, 1350
DpnI GATC 6 cut(s) 84, 231, 330, 383, 449, 536
DpnII GATC 6 cut(s) 82, 229, 328, 381, 447, 534
DrdI GACNNNNNNGTC 1 cut(s) 296
DseDI GACNNNNNNGTC 1 cut(s) 296
EaeI YGGCCR 2 cut(s) 465, 1095
Eam1104I CTCTTC 3 cut(s) 440, 446, 608
EarI CTCTTC 3 cut(s) 440, 446, 608
EciI GGCGGA 4 cut(s) 88, 103, 238, 565
Eco130I CCWWGG 1 cut(s) 1434
Eco31I GGTCTC 2 cut(s) 577, 736
Eco47I GGWCC 3 cut(s) 364, 719, 1103
Eco88I CYCGRG 2 cut(s) 855, 1148
EcoO109I RGGNCCY 1 cut(s) 364
EcoRI GAATTC 1 cut(s) 523
EcoRII CCWGG 2 cut(s) 74, 1074
EcoT14I CCWWGG 1 cut(s) 1434
EgeI GGCGCC 3 cut(s) 295, 915, 1350
EheI GGCGCC 3 cut(s) 295, 915, 1350
ErhI CCWWGG 1 cut(s) 1434
Esp3I CGTCTC 1 cut(s) 431
FaeI CATG 4 cut(s) 145, 379, 955, 1438
FaqI GGGAC 4 cut(s) 22, 92, 526, 733
FatI CATG 4 cut(s) 141, 375, 951, 1434
FauI CCCGC 3 cut(s) 35, 56, 427
FblI GTMKAC 2 cut(s) 513, 1071
HaeII RGCGCY 5 cut(s) 297, 502, 917, 1352, 1398
HaeIII GGCC 3 cut(s) 175, 467, 1097
HgaI GACGC 5 cut(s) 344, 413, 524, 751, 1172
Hin1I GRCGYC 5 cut(s) 294, 336, 516, 914, 1349
Hin1II CATG 4 cut(s) 145, 379, 955, 1438
HincII GTYRAC 1 cut(s) 514
HindII GTYRAC 1 cut(s) 514
HindIII AAGCTT 1 cut(s) 887
HinfI GANTC 9 cut(s) 23, 32, 485, 609, 662, 689, 760, 772, 1402
HphI GGTGA 1 cut(s) 343
Hpy166II GTNNAC 6 cut(s) 514, 1045, 1072, 1156, 1263, 1300
Hpy8I GTNNAC 6 cut(s) 514, 1045, 1072, 1156, 1263, 1300
HpyAV CCTTC 4 cut(s) 262, 465, 542, 1250
HpyCH4III ACNGT 5 cut(s) 682, 1160, 1253, 1345, 1418
HpyCH4IV ACGT 1 cut(s) 198
HpyCH4V TGCA 4 cut(s) 147, 793, 955, 1373
HpyF3I CTNAG 4 cut(s) 414, 428, 882, 1194
HpySE526I ACGT 1 cut(s) 198
Hsp92I GRCGYC 5 cut(s) 294, 336, 516, 914, 1349
Hsp92II CATG 4 cut(s) 145, 379, 955, 1438
KasI GGCGCC 3 cut(s) 293, 913, 1348
KroI GCCGGC 1 cut(s) 943
KroNI GCCGGC 1 cut(s) 945
Kzo9I GATC 6 cut(s) 82, 229, 328, 381, 447, 534
LguI GCTCTTC 1 cut(s) 440
LweI GCATC 2 cut(s) 246, 1360
MaeII ACGT 1 cut(s) 198
MaeIII GTNAC 3 cut(s) 349, 806, 1160
MalI GATC 6 cut(s) 84, 231, 330, 383, 449, 536
MbiI CCGCTC 3 cut(s) 461, 641, 929
MboI GATC 6 cut(s) 82, 229, 328, 381, 447, 534
MboII GAAGA 4 cut(s) 427, 433, 595, 712
MflI RGATCY 2 cut(s) 328, 534
MluCI AATT 8 cut(s) 523, 653, 725, 752, 795, 956, 1282, 1407
Mly113I GGCGCC 3 cut(s) 294, 914, 1349
MlyI GAGTC 3 cut(s) 17, 41, 494
MmeI TCCRAC 3 cut(s) 504, 701, 986
MroNI GCCGGC 1 cut(s) 943
MspA1I CMGCKG 2 cut(s) 869, 1064
MspR9I CCNGG 9 cut(s) 20, 76, 172, 321, 545, 921, 1023, 1076, 1288
Mva1269I GAATGC 1 cut(s) 795
MvaI CCWGG 2 cut(s) 76, 1076
MvnI CGCG 9 cut(s) 30, 51, 405, 422, 424, 566, 650, 671, 1322
NaeI GCCGGC 1 cut(s) 945
NarI GGCGCC 3 cut(s) 294, 914, 1349
NciI CCSGG 7 cut(s) 20, 172, 321, 545, 921, 1023, 1288
NcoI CCATGG 1 cut(s) 1434
NdeII GATC 6 cut(s) 82, 229, 328, 381, 447, 534
NgoMIV GCCGGC 1 cut(s) 943
NlaIII CATG 4 cut(s) 145, 379, 955, 1438
NmuCI GTSAC 3 cut(s) 349, 806, 1160
NruI TCGCGA 1 cut(s) 650
NspI RCATGY 1 cut(s) 955
PciSI GCTCTTC 1 cut(s) 440
PcsI WCGNNNNNNNCGW 3 cut(s) 204, 366, 480
PctI GAATGC 1 cut(s) 795
PdiI GCCGGC 1 cut(s) 945
PfeI GAWTC 6 cut(s) 609, 662, 689, 760, 772, 1402
PflFI GACNNNGTC 1 cut(s) 745
PflMI CCANNNNNTGG 1 cut(s) 1345
PfoI TCCNGGA 1 cut(s) 1286
PinAI ACCGGT 1 cut(s) 1357
Ple19I CGATCG 1 cut(s) 232
PleI GAGTC 3 cut(s) 17, 40, 493
PluTI GGCGCC 3 cut(s) 297, 917, 1352
PpsI GAGTC 3 cut(s) 17, 40, 493
PpuMI RGGWCCY 1 cut(s) 364
Psp5II RGGWCCY 1 cut(s) 364
Psp6I CCWGG 2 cut(s) 74, 1074
PspGI CCWGG 2 cut(s) 74, 1074
PspPI GGNCC 4 cut(s) 174, 364, 719, 1103
PspPPI RGGWCCY 1 cut(s) 364
PsrI GAACNNNNNNTAC 1 cut(s) 31
PsuI RGATCY 2 cut(s) 328, 534
PsyI GACNNNGTC 1 cut(s) 745
PvuI CGATCG 1 cut(s) 232
PvuII CAGCTG 2 cut(s) 869, 1064
RruI TCGCGA 1 cut(s) 650
RsaI GTAC 6 cut(s) 311, 389, 1046, 1157, 1301, 1356
RsaNI GTAC 6 cut(s) 310, 388, 1045, 1156, 1300, 1355
Rsr2I CGGWCCG 1 cut(s) 719
RsrII CGGWCCG 1 cut(s) 719
SalI GTCGAC 1 cut(s) 512
SapI GCTCTTC 1 cut(s) 440
Sau3AI GATC 6 cut(s) 82, 229, 328, 381, 447, 534
Sau96I GGNCC 4 cut(s) 174, 364, 719, 1103
SchI GAGTC 3 cut(s) 17, 41, 494
ScrFI CCNGG 9 cut(s) 20, 76, 172, 321, 545, 921, 1023, 1076, 1288
SfaNI GCATC 2 cut(s) 246, 1360
SfcI CTRYAG 1 cut(s) 1308
SfoI GGCGCC 3 cut(s) 295, 915, 1350
SgrDI CGTCGACG 1 cut(s) 512
SinI GGWCC 3 cut(s) 364, 719, 1103
Sse9I AATT 8 cut(s) 523, 653, 725, 752, 795, 956, 1282, 1407
SspDI GGCGCC 3 cut(s) 293, 913, 1348
StyD4I CCNGG 9 cut(s) 18, 74, 170, 319, 543, 919, 1021, 1074, 1286
StyI CCWWGG 1 cut(s) 1434
TaaI ACNGT 5 cut(s) 682, 1160, 1253, 1345, 1418
TaiI ACGT 1 cut(s) 201
TaqI TCGA 8 cut(s) 57, 228, 333, 369, 384, 513, 612, 756
TasI AATT 8 cut(s) 523, 653, 725, 752, 795, 956, 1282, 1407
TatI WGTACW 1 cut(s) 1299
TfiI GAWTC 6 cut(s) 609, 662, 689, 760, 772, 1402
TscAI CASTG 5 cut(s) 509, 1000, 1105, 1191, 1258
TseFI GTSAC 3 cut(s) 349, 806, 1160
Tsp45I GTSAC 3 cut(s) 349, 806, 1160
TspDTI ATGAA 2 cut(s) 244, 1320
TspGWI ACGGA 3 cut(s) 337, 517, 655
TspRI CASTG 5 cut(s) 509, 1000, 1105, 1191, 1258
Tth111I GACNNNGTC 1 cut(s) 745
Van91I CCANNNNNTGG 1 cut(s) 1345
VpaK11BI GGWCC 3 cut(s) 364, 719, 1103
XapI RAATTY 2 cut(s) 523, 1407
XceI RCATGY 1 cut(s) 955
XmiI GTMKAC 2 cut(s) 513, 1071
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.