pycom05g04200

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0002525
Physical Location & Seq
Reverse (-)
4938281 .. 4940369
2089 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g04200.1

Sequence Viewer

Length: 1422 bp
ATGGAAAACTACTCATACAACTCCTATCCCGAGTCCGGTAACTCGTCCCCACGGTCCCGAGATATCGATTTCGAGAACACTCCGCCATGGGAGGTCGACGCAGCCAATTCTCAGACCCAAAGCTACAAGGCCAAGTTCATGTGCAGCTACGGCGGAAAGATCGATCTGCCTCGCCCGCACGACAACCAGCTCTGCTACGTCGGCGGTGAGACCAAGATTTTCTCCGTCGATCGCAACATCAAGTTCGCCGCCATCATTTCCAAGCTCTCCTCCCTCTCCGAGTACGACGTGTCGTTCAAGTACCAACTCCCCGGCGAAGACCTCGACGCCTTGATCTCCGTTACCAACGACGACGACTTGGAGCACATGATGCACGAGAACGATCGCCTCTACCGGGCTTCCACTAGGCCCGCCCGCATGAGATTGTTCCTCTTTAATGCGAGCCCCAACGGTGGATTCGGGTCGGATGATGGAAGGTCCAATAAGGATCGGTTCGTCGAGGCTCTTAATTCGGGTCCCACCCAGGCGCCCTACCTAAATTCCAAGCCTCCGGTCCAAAATAATGTGGAATTTCTCTGCGGCATGGAAAAGGGTGTCGTCCCTGCTGCCGTGGCGCCTCCTCCTCCTCCAGCGCCGATACCGGAACAAGTTGCGCCCCCGCCTGACTTTGTCAGATCGGGCCACACAGAACGGGTTATCGGATCGGATCCGGTGGTGAGTCCGGTGGAGTTCCAAAGGCAATTGCAGAGGCTACAAATCGGAGAACAAGATCAGCAAGCTATGTACGGGAGAAAGAACGACGACAATCTCGTTGGAGGCTATAGCGGCTCTGGAGATTATTACGTACAGAAGATGCCGGAGAAGGCTCCTCCGGTGACGGCTGCGCCGCCAGCTGGCTACTGGCCGGAGATTACTAGCGGTGGGTTTCCGGGGATAGGAGCTCCGGAACAGCCGATGTACATGTTTACATCCGCTCCAGGAGGCGGGGCAGTGTACCACGCACAGCCACCGATGGTACGACCGGTAAACGGGCCACCGGGTCAAGAGTACTATCACGTGCAGCAGCGAGTGGGGTCTGAAATTTACCGGGAACAGCCGCCGAGGTACAATGTAGTGCCACAACTGCAGCAGCAGCAGCCGAGTACTCTCCCACCACAGCCGCCAAAATACGTGGCGACGTACGCCGACACAACACCGTATGCACAGGTGGCGTACGATAGCACGACGGGGAGGCAGGTATACTATAGTGCGCCGGGAGGGGTTGTGCAGCAACAGCAGCAGGCGTATCAAGCCGTGGGCCCCGCAGCGGTGGGCGGAGAAATGCGAGCGGCTGGCGCGTTGGGTCAGGAGGAATTGTCAAATGGGTACAATCTCCGGTGCCAAATCTTTGATCTGGATATAATTCGCAAAAACAAGCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

474

Amino Acids

52.04

Weight (kDa)

5.24

Isoelectric Point (pI)

54.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 60 - 146 2.6e-16 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1225
AccB1I GGYRCC 3 cut(s) 526, 613, 1377
AccBSI CCGCTC 2 cut(s) 974, 1328
AccI GTMKAC 2 cut(s) 96, 1239
AccII CGCG 1 cut(s) 1337
AccIII TCCGGA 1 cut(s) 943
AclWI GGATC 4 cut(s) 495, 701, 709, 714
AcoI YGGCCR 1 cut(s) 902
AcsI RAATTY 3 cut(s) 538, 569, 1080
AcvI CACGTG 1 cut(s) 1057
AcyI GRCGYC 3 cut(s) 327, 527, 614
AfiI CCNNNNNNNGG 8 cut(s) 35, 394, 452, 893, 935, 983, 1028, 1306
AflIII ACRYGT 2 cut(s) 288, 960
AgeI ACCGGT 1 cut(s) 1021
AgsI TTSAA 1 cut(s) 298
AjiI CACGTC 1 cut(s) 289
AjnI CCWGG 2 cut(s) 522, 976
AjuI GAANNNNNNNTTGG 2 cut(s) 440, 472
AluBI AGCT 7 cut(s) 123, 147, 190, 265, 779, 893, 941
AluI AGCT 7 cut(s) 123, 147, 190, 265, 779, 893, 941
Alw21I GWGCWC 2 cut(s) 366, 943
Alw26I GTCTC 1 cut(s) 203
AlwI GGATC 4 cut(s) 495, 701, 709, 714
Ama87I CYCGRG 2 cut(s) 29, 57
Aor13HI TCCGGA 1 cut(s) 943
AoxI GGCC 6 cut(s) 129, 407, 679, 902, 1031, 1297
ApaI GGGCCC 1 cut(s) 1301
ApoI RAATTY 3 cut(s) 538, 569, 1080
AsiGI ACCGGT 1 cut(s) 1021
AspLEI GCGC 7 cut(s) 529, 616, 634, 655, 886, 1252, 1337
AspS9I GGNCC 9 cut(s) 54, 408, 477, 515, 553, 679, 1031, 1297, 1298
AsuC2I CCSGG 6 cut(s) 312, 395, 930, 1038, 1088, 1254
AsuHPI GGTGA 3 cut(s) 218, 727, 886
AvaI CYCGRG 2 cut(s) 29, 57
AvaII GGWCC 4 cut(s) 54, 477, 515, 553
BaeGI GKGCMC 1 cut(s) 1301
BamHI GGATCC 1 cut(s) 706
BanI GGYRCC 3 cut(s) 526, 613, 1377
BanII GRGCYC 3 cut(s) 446, 943, 1301
BauI CACGAG 1 cut(s) 374
BbrPI CACGTG 1 cut(s) 1057
BbsI GAAGAC 1 cut(s) 324
Bbv12I GWGCWC 2 cut(s) 366, 943
BccI CCATC 3 cut(s) 260, 464, 1006
BceAI ACGGC 4 cut(s) 166, 593, 894, 1277
BciT130I CCWGG 2 cut(s) 524, 978
BcnI CCSGG 6 cut(s) 312, 395, 930, 1038, 1088, 1254
BcoDI GTCTC 1 cut(s) 203
BfaI CTAG 2 cut(s) 405, 915
BfmI CTRYAG 3 cut(s) 820, 1124, 1243
BfoI RGCGCY 3 cut(s) 530, 617, 635
BfuAI ACCTGC 1 cut(s) 1225
BmcAI AGTACT 2 cut(s) 1049, 1144
Bme1390I CCNGG 8 cut(s) 312, 395, 524, 930, 978, 1038, 1088, 1254
Bme18I GGWCC 4 cut(s) 54, 477, 515, 553
BmeT110I CYCGRG 2 cut(s) 29, 57
BmgBI CACGTC 1 cut(s) 289
BmgT120I GGNCC 9 cut(s) 54, 408, 477, 515, 553, 679, 1031, 1297, 1298
BmrFI CCNGG 8 cut(s) 312, 395, 524, 930, 978, 1038, 1088, 1254
BmsI GCATC 2 cut(s) 360, 843
BpiI GAAGAC 1 cut(s) 324
BpmI CTGGAG 3 cut(s) 612, 852, 960
BpuMI CCSGG 6 cut(s) 312, 395, 930, 1038, 1088, 1254
Bsa29I ATCGAT 2 cut(s) 66, 162
BsaAI YACGTR 3 cut(s) 844, 1057, 1171
BsaHI GRCGYC 3 cut(s) 327, 527, 614
BsaI GGTCTC 1 cut(s) 203
BsaJI CCNNGG 8 cut(s) 50, 86, 310, 522, 609, 929, 1100, 1293
BsaWI WCCGGW 9 cut(s) 35, 550, 640, 709, 721, 871, 943, 1021, 1374
BsaXI ACNNNNNCTCC 6 cut(s) 958, 988, 1248, 1278, 1340, 1370
Bsc4I CCNNNNNNNGG 8 cut(s) 35, 394, 452, 893, 935, 983, 1028, 1306
Bse118I RCCGGY 1 cut(s) 1021
Bse1I ACTGG 1 cut(s) 905
BseAI TCCGGA 1 cut(s) 943
BseBI CCWGG 2 cut(s) 524, 978
BseCI ATCGAT 2 cut(s) 66, 162
BseDI CCNNGG 8 cut(s) 50, 86, 310, 522, 609, 929, 1100, 1293
BseGI GGATG 2 cut(s) 472, 968
BseLI CCNNNNNNNGG 8 cut(s) 35, 394, 452, 893, 935, 983, 1028, 1306
BseMII CTCAG 1 cut(s) 125
BseNI ACTGG 1 cut(s) 905
BseRI GAGGAG 5 cut(s) 259, 609, 612, 615, 858
BseSI GKGCMC 1 cut(s) 1301
BsgI GTGCAG 3 cut(s) 163, 1079, 1286
Bsh1236I CGCG 1 cut(s) 1337
Bsh1285I CGRYCG 3 cut(s) 232, 385, 1022
BshFI GGCC 6 cut(s) 131, 409, 681, 904, 1033, 1299
BshNI GGYRCC 3 cut(s) 526, 613, 1377
BshTI ACCGGT 1 cut(s) 1021
BshVI ATCGAT 2 cut(s) 66, 162
BsiEI CGRYCG 3 cut(s) 232, 385, 1022
BsiHKAI GWGCWC 2 cut(s) 366, 943
BsiHKCI CYCGRG 2 cut(s) 29, 57
BsiWI CGTACG 2 cut(s) 1179, 1212
BslFI GGGAC 4 cut(s) 31, 40, 501, 584
BslI CCNNNNNNNGG 8 cut(s) 35, 394, 452, 893, 935, 983, 1028, 1306
BsmAI GTCTC 1 cut(s) 203
BsmFI GGGAC 4 cut(s) 31, 40, 501, 584
BsnI GGCC 6 cut(s) 131, 409, 681, 904, 1033, 1299
Bso31I GGTCTC 1 cut(s) 203
BsoBI CYCGRG 2 cut(s) 29, 57
Bsp120I GGGCCC 1 cut(s) 1297
Bsp1286I GDGCHC 4 cut(s) 366, 446, 943, 1301
Bsp13I TCCGGA 1 cut(s) 943
Bsp1407I TGTACA 1 cut(s) 957
Bsp19I CCATGG 1 cut(s) 86
BspANI GGCC 6 cut(s) 131, 409, 681, 904, 1033, 1299
BspCNI CTCAG 1 cut(s) 124
BspDI ATCGAT 2 cut(s) 66, 162
BspEI TCCGGA 1 cut(s) 943
BspFNI CGCG 1 cut(s) 1337
BspMAI CTGCAG 1 cut(s) 1128
BspMI ACCTGC 1 cut(s) 1225
BspPI GGATC 4 cut(s) 495, 701, 709, 714
BspT107I GGYRCC 3 cut(s) 526, 613, 1377
BspTNI GGTCTC 1 cut(s) 203
BsrBI CCGCTC 2 cut(s) 974, 1328
BsrFI RCCGGY 1 cut(s) 1021
BsrGI TGTACA 1 cut(s) 957
BsrI ACTGG 1 cut(s) 905
BssAI RCCGGY 1 cut(s) 1021
BssECI CCNNGG 8 cut(s) 50, 86, 310, 522, 609, 929, 1100, 1293
BssNAI GTATAC 1 cut(s) 1240
BssNI GRCGYC 3 cut(s) 327, 527, 614
BssSI CACGAG 1 cut(s) 374
BssT1I CCWWGG 1 cut(s) 86
Bst1107I GTATAC 1 cut(s) 1240
Bst2BI CACGAG 1 cut(s) 374
Bst2UI CCWGG 2 cut(s) 524, 978
Bst4CI ACNGT 3 cut(s) 54, 452, 1197
BstACI GRCGYC 3 cut(s) 327, 527, 614
BstAPI GCANNNNNTGC 1 cut(s) 370
BstAUI TGTACA 1 cut(s) 957
BstBAI YACGTR 3 cut(s) 844, 1057, 1171
BstDEI CTNAG 1 cut(s) 111
BstDSI CCRYGG 4 cut(s) 50, 86, 609, 1293
BstF5I GGATG 2 cut(s) 472, 968
BstFNI CGCG 1 cut(s) 1337
BstH2I RGCGCY 3 cut(s) 530, 617, 635
BstHHI GCGC 7 cut(s) 529, 616, 634, 655, 886, 1252, 1337
BstMAI GTCTC 1 cut(s) 203
BstMCI CGRYCG 3 cut(s) 232, 385, 1022
BstNI CCWGG 2 cut(s) 524, 978
BstNSI RCATGY 1 cut(s) 964
BstSCI CCNGG 8 cut(s) 310, 393, 522, 928, 976, 1036, 1086, 1252
BstSFI CTRYAG 3 cut(s) 820, 1124, 1243
BstSLI GKGCMC 1 cut(s) 1301
BstSNI TACGTA 1 cut(s) 844
BstUI CGCG 1 cut(s) 1337
BstV2I GAAGAC 1 cut(s) 324
BstX2I RGATCY 1 cut(s) 706
BstYI RGATCY 1 cut(s) 706
BstZ17I GTATAC 1 cut(s) 1240
Bsu15I ATCGAT 2 cut(s) 66, 162
BsuRI GGCC 6 cut(s) 131, 409, 681, 904, 1033, 1299
BsuTUI ATCGAT 2 cut(s) 66, 162
BtgI CCRYGG 4 cut(s) 50, 86, 609, 1293
BtrI CACGTC 1 cut(s) 289
BtsCI GGATG 2 cut(s) 472, 968
BtsI GCAGTG 1 cut(s) 996
BtsIMutI CAGTG 1 cut(s) 996
BveI ACCTGC 1 cut(s) 1225
CfoI GCGC 7 cut(s) 529, 616, 634, 655, 886, 1252, 1337
Cfr10I RCCGGY 1 cut(s) 1021
Cfr13I GGNCC 9 cut(s) 54, 408, 477, 515, 553, 679, 1031, 1297, 1298
ClaI ATCGAT 2 cut(s) 66, 162
CseI GACGC 2 cut(s) 107, 335
CspAI ACCGGT 1 cut(s) 1021
CspCI CAANNNNNGTGG 2 cut(s) 1152, 1187
CviAII CATG 6 cut(s) 87, 139, 367, 418, 583, 961
DdeI CTNAG 1 cut(s) 111
DinI GGCGCC 2 cut(s) 528, 615
EaeI YGGCCR 1 cut(s) 902
EciI GGCGGA 3 cut(s) 72, 168, 1329
Ecl136II GAGCTC 1 cut(s) 941
Eco105I TACGTA 1 cut(s) 844
Eco130I CCWWGG 1 cut(s) 86
Eco24I GRGCYC 3 cut(s) 446, 943, 1301
Eco31I GGTCTC 1 cut(s) 203
Eco32I GATATC 1 cut(s) 64
Eco47I GGWCC 4 cut(s) 54, 477, 515, 553
Eco53kI GAGCTC 1 cut(s) 941
Eco72I CACGTG 1 cut(s) 1057
Eco88I CYCGRG 2 cut(s) 29, 57
EcoICRI GAGCTC 1 cut(s) 941
EcoO109I RGGNCCY 2 cut(s) 515, 1298
EcoRII CCWGG 2 cut(s) 522, 976
EcoRV GATATC 1 cut(s) 64
EcoT14I CCWWGG 1 cut(s) 86
EcoT38I GRGCYC 3 cut(s) 446, 943, 1301
EgeI GGCGCC 2 cut(s) 528, 615
EheI GGCGCC 2 cut(s) 528, 615
ErhI CCWWGG 1 cut(s) 86
FaeI CATG 6 cut(s) 90, 142, 370, 421, 586, 964
FaqI GGGAC 4 cut(s) 31, 40, 501, 584
FatI CATG 6 cut(s) 86, 138, 366, 417, 582, 960
FauI CCCGC 6 cut(s) 183, 418, 422, 666, 977, 1309
FblI GTMKAC 2 cut(s) 96, 1239
FokI GGATG 2 cut(s) 479, 955
FriOI GRGCYC 3 cut(s) 446, 943, 1301
FspBI CTAG 2 cut(s) 405, 915
GlaI GCGC 7 cut(s) 528, 615, 633, 654, 885, 1251, 1336
GsuI CTGGAG 3 cut(s) 612, 852, 960
HaeII RGCGCY 3 cut(s) 530, 617, 635
HaeIII GGCC 6 cut(s) 131, 409, 681, 904, 1033, 1299
HgaI GACGC 2 cut(s) 107, 335
HhaI GCGC 7 cut(s) 529, 616, 634, 655, 886, 1252, 1337
Hin1I GRCGYC 3 cut(s) 327, 527, 614
Hin1II CATG 6 cut(s) 90, 142, 370, 421, 586, 964
Hin6I GCGC 7 cut(s) 527, 614, 632, 653, 884, 1250, 1335
HinP1I GCGC 7 cut(s) 527, 614, 632, 653, 884, 1250, 1335
HincII GTYRAC 1 cut(s) 97
HindII GTYRAC 1 cut(s) 97
HinfI GANTC 3 cut(s) 32, 456, 718
HphI GGTGA 3 cut(s) 218, 727, 886
Hpy166II GTNNAC 5 cut(s) 97, 966, 994, 1027, 1240
Hpy188I TCNGA 8 cut(s) 114, 280, 466, 674, 701, 706, 761, 1078
Hpy188III TCNNGA 8 cut(s) 29, 57, 73, 831, 944, 1043, 1346, 1394
Hpy8I GTNNAC 5 cut(s) 97, 966, 994, 1027, 1240
HpyAV CCTTC 2 cut(s) 468, 856
HpyCH4III ACNGT 3 cut(s) 54, 452, 1197
HpyCH4IV ACGT 6 cut(s) 198, 288, 843, 1056, 1170, 1178
HpyCH4V TGCA 7 cut(s) 144, 373, 745, 1060, 1126, 1202, 1267
HpyF3I CTNAG 1 cut(s) 111
HpySE526I ACGT 6 cut(s) 198, 288, 843, 1056, 1170, 1178
Hsp92I GRCGYC 3 cut(s) 327, 527, 614
Hsp92II CATG 6 cut(s) 90, 142, 370, 421, 586, 964
HspAI GCGC 7 cut(s) 527, 614, 632, 653, 884, 1250, 1335
KasI GGCGCC 2 cut(s) 526, 613
KflI GGGWCCC 1 cut(s) 515
Kpn2I TCCGGA 1 cut(s) 943
LmnI GCTCC 5 cut(s) 361, 871, 938, 946, 979
LweI GCATC 2 cut(s) 360, 843
MaeI CTAG 2 cut(s) 405, 915
MaeII ACGT 6 cut(s) 198, 288, 843, 1056, 1170, 1178
MaeIII GTNAC 3 cut(s) 38, 340, 874
MbiI CCGCTC 2 cut(s) 974, 1328
MboII GAAGA 2 cut(s) 329, 862
MfeI CAATTG 1 cut(s) 740
MflI RGATCY 1 cut(s) 706
MhlI GDGCHC 4 cut(s) 366, 446, 943, 1301
MluCI AATT 8 cut(s) 106, 508, 538, 569, 740, 1080, 1352, 1401
Mly113I GGCGCC 2 cut(s) 527, 614
MlyI GAGTC 2 cut(s) 41, 727
MmeI TCCRAC 2 cut(s) 444, 793
MroI TCCGGA 1 cut(s) 943
MseI TTAA 3 cut(s) 435, 507, 1420
MspA1I CMGCKG 2 cut(s) 893, 1307
MspR9I CCNGG 8 cut(s) 312, 395, 524, 930, 978, 1038, 1088, 1254
MunI CAATTG 1 cut(s) 740
MvaI CCWGG 2 cut(s) 524, 978
MvnI CGCG 1 cut(s) 1337
NarI GGCGCC 2 cut(s) 527, 614
NciI CCSGG 6 cut(s) 312, 395, 930, 1038, 1088, 1254
NcoI CCATGG 1 cut(s) 86
NlaIII CATG 6 cut(s) 90, 142, 370, 421, 586, 964
NmeAIII GCCGAG 2 cut(s) 1125, 1164
NmuCI GTSAC 1 cut(s) 874
NspI RCATGY 1 cut(s) 964
PciI ACATGT 1 cut(s) 960
PcsI WCGNNNNNNNCGW 1 cut(s) 807
PfeI GAWTC 1 cut(s) 456
Pfl23II CGTACG 2 cut(s) 1179, 1212
PflFI GACNNNGTC 1 cut(s) 668
PfoI TCCNGGA 1 cut(s) 976
PinAI ACCGGT 1 cut(s) 1021
Ple19I CGATCG 2 cut(s) 232, 385
PleI GAGTC 2 cut(s) 40, 726
PluTI GGCGCC 2 cut(s) 530, 617
PmaCI CACGTG 1 cut(s) 1057
PmlI CACGTG 1 cut(s) 1057
PpsI GAGTC 2 cut(s) 40, 726
Ppu21I YACGTR 3 cut(s) 844, 1057, 1171
PpuMI RGGWCCY 1 cut(s) 515
PscI ACATGT 1 cut(s) 960
Psp124BI GAGCTC 1 cut(s) 943
Psp5II RGGWCCY 1 cut(s) 515
Psp6I CCWGG 2 cut(s) 522, 976
PspCI CACGTG 1 cut(s) 1057
PspGI CCWGG 2 cut(s) 522, 976
PspLI CGTACG 2 cut(s) 1179, 1212
PspOMI GGGCCC 1 cut(s) 1297
PspPI GGNCC 9 cut(s) 54, 408, 477, 515, 553, 679, 1031, 1297, 1298
PspPPI RGGWCCY 1 cut(s) 515
PstI CTGCAG 1 cut(s) 1128
PsuI RGATCY 1 cut(s) 706
PsyI GACNNNGTC 1 cut(s) 668
PvuI CGATCG 2 cut(s) 232, 385
PvuII CAGCTG 1 cut(s) 893
SacI GAGCTC 1 cut(s) 943
SalI GTCGAC 1 cut(s) 95
SaqAI TTAA 3 cut(s) 435, 507, 1420
Sau96I GGNCC 9 cut(s) 54, 408, 477, 515, 553, 679, 1031, 1297, 1298
ScaI AGTACT 2 cut(s) 1049, 1144
SchI GAGTC 2 cut(s) 41, 727
ScrFI CCNGG 8 cut(s) 312, 395, 524, 930, 978, 1038, 1088, 1254
SduI GDGCHC 4 cut(s) 366, 446, 943, 1301
SfaNI GCATC 2 cut(s) 360, 843
SfcI CTRYAG 3 cut(s) 820, 1124, 1243
SfoI GGCGCC 2 cut(s) 528, 615
SinI GGWCC 4 cut(s) 54, 477, 515, 553
SnaBI TACGTA 1 cut(s) 844
Sse9I AATT 8 cut(s) 106, 508, 538, 569, 740, 1080, 1352, 1401
SspDI GGCGCC 2 cut(s) 526, 613
SspMI CTAG 2 cut(s) 405, 915
SstI GAGCTC 1 cut(s) 943
StyD4I CCNGG 8 cut(s) 310, 393, 522, 928, 976, 1036, 1086, 1252
StyI CCWWGG 1 cut(s) 86
TaaI ACNGT 3 cut(s) 54, 452, 1197
TaiI ACGT 6 cut(s) 201, 291, 846, 1059, 1173, 1181
TaqI TCGA 7 cut(s) 66, 72, 96, 162, 228, 324, 498
TasI AATT 8 cut(s) 106, 508, 538, 569, 740, 1080, 1352, 1401
TatI WGTACW 3 cut(s) 957, 1047, 1142
TauI GCSGC 7 cut(s) 251, 582, 828, 889, 1099, 1162, 1331
TfiI GAWTC 1 cut(s) 456
Tru1I TTAA 3 cut(s) 435, 507, 1420
Tru9I TTAA 3 cut(s) 435, 507, 1420
TscAI CASTG 1 cut(s) 996
TseFI GTSAC 1 cut(s) 874
Tsp45I GTSAC 1 cut(s) 874
TspDTI ATGAA 1 cut(s) 127
TspGWI ACGGA 2 cut(s) 214, 328
TspRI CASTG 1 cut(s) 996
Tth111I GACNNNGTC 1 cut(s) 668
VpaK11BI GGWCC 4 cut(s) 54, 477, 515, 553
XapI RAATTY 3 cut(s) 538, 569, 1080
XceI RCATGY 1 cut(s) 964
XcmI CCANNNNNNNNNTGG 1 cut(s) 897
XmiI GTMKAC 2 cut(s) 96, 1239
XspI CTAG 2 cut(s) 405, 915
ZrmI AGTACT 2 cut(s) 1049, 1144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.