pycom15g35330

PB1 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0002525
Physical Location & Seq
Forward (+)
35262917 .. 35263978
1062 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g35330.1

Sequence Viewer

Length: 1062 bp
ATGATGGTCGAGTACGACCGGCTGTACCGCGCTTCAGCTAAGCCCGCCAGGCTCCGACTCTTTCTCTTCCCTCTTGACAGCAACACTTCCGCTAGCTTCGGCTCCAGCGAGACCAAGTCCGAGCGCCAGTGGTTCGTCGACGCTTTGAATTCGGTTCAGATCGGGTCTCCCGATGGATTCTCTCCGACGGCTCCGGTCACGGCCTCGCCGACAAACCCCGATTTCCTGTTCGGATTCGATAAGGGATACACGGCTGTACCGGTTGCGAAATTTCCCGACTCGGTCACTCCGCCGACGGTTCCCGATTTGATTGTGAAAAGCACTCTGGTTGGATCTGGGGATCGCCACACCGGAGAAGCGGTGATGTCTCAGGCGGAGATTCAGAGGCAGATCCAGGAGTTGCAGAGGATGCAAATCGTTGCTGGTCACGAGCCGACCACCATCTATCACCGGAAAAGCGACGAAATGGGAAACCCTAGGGGTTACACCGGCGATTATTACACACAGAAACTTCCAGAGAACATTCAACCGGCGCAAGGATCGTCTCCGGTGGGAGTGCCACAACCTGTGCCATTTCCAACTGCGTATATGCCAGAGAGGCACATGAACACTGGGGGTTATCCGGTGACGACTGCCCCATCTGGACCGGAGCAGCCCGTTTATTTCATTTCTACTCCGACCGGTGTATACCAGGCTCCCGCCCCTAGACCGGCGACCGGTCCAGTTGGTCAGGCATACTATGGAGTACCGCAGAGGATGGCACCAGAGGTGTACCAGGACGCTCAGGTGTACAATACTGCCGTGGCAGCACAGCAAGCACAACAAGCTCATCAGGCACAACAAATTCAGCAGGCTGCAGCTTATAGTGAAGGGTTTGCACAGGTTGGGTATGATGCCGTCGGGAGGCAGGTATACTACACTGCAGCAGGAGGTGTCGTTTCTTCATATCAGACGGTTGCTCCGGTGCCCGTAGATGGGAGACAAGCTGCTGCTGGTGTATTGAATCAAATTCAAGATGGTAAGGTTGTTGTTTCCATGGCACCCCAAGCGTCTGCTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

354

Amino Acids

37.9

Weight (kDa)

5.56

Isoelectric Point (pI)

48.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 898
AccB1I GGYRCC 3 cut(s) 760, 964, 1039
AccI GTMKAC 3 cut(s) 138, 687, 912
AccII CGCG 1 cut(s) 30
AciI CCGC 8 cut(s) 28, 45, 90, 290, 359, 374, 699, 749
AclWI GGATC 4 cut(s) 340, 348, 385, 547
AcsI RAATTY 4 cut(s) 148, 269, 843, 1008
AcuI CTGAAG 1 cut(s) 18
AfaI GTAC 6 cut(s) 14, 26, 258, 747, 773, 791
AfiI CCNNNNNNNGG 6 cut(s) 536, 709, 716, 903, 974, 975
AgeI ACCGGT 3 cut(s) 259, 680, 716
AgsI TTSAA 4 cut(s) 148, 527, 1003, 1013
AjnI CCWGG 4 cut(s) 47, 393, 690, 774
AluBI AGCT 5 cut(s) 38, 96, 827, 860, 986
AluI AGCT 5 cut(s) 38, 96, 827, 860, 986
Alw26I GTCTC 5 cut(s) 104, 171, 372, 549, 973
AlwI GGATC 4 cut(s) 340, 348, 385, 547
AoxI GGCC 1 cut(s) 201
ApeKI GCWGC 7 cut(s) 652, 806, 854, 857, 923, 986, 989
ApoI RAATTY 4 cut(s) 148, 269, 843, 1008
AsiGI ACCGGT 3 cut(s) 259, 680, 716
AspA2I CCTAGG 1 cut(s) 476
AspLEI GCGC 3 cut(s) 32, 126, 535
AspS9I GGNCC 2 cut(s) 644, 719
AsuHPI GGTGA 3 cut(s) 373, 440, 637
AsuNHI GCTAGC 1 cut(s) 92
AvaII GGWCC 2 cut(s) 644, 719
AvrII CCTAGG 1 cut(s) 476
BaeGI GKGCMC 1 cut(s) 969
BaeI ACNNNNGTAYC 2 cut(s) 240, 273
BanI GGYRCC 3 cut(s) 760, 964, 1039
BauI CACGAG 1 cut(s) 428
BbvI GCAGC 7 cut(s) 664, 818, 841, 869, 935, 973, 976
BccI CCATC 6 cut(s) 167, 449, 646, 751, 968, 1010
BceAI ACGGC 5 cut(s) 204, 216, 267, 785, 881
BciT130I CCWGG 4 cut(s) 49, 395, 692, 776
BciVI GTATCC 1 cut(s) 239
BcoDI GTCTC 5 cut(s) 104, 171, 372, 549, 973
BfaI CTAG 3 cut(s) 93, 477, 705
BfmI CTRYAG 2 cut(s) 855, 921
BfoI RGCGCY 1 cut(s) 127
BfuAI ACCTGC 1 cut(s) 898
BfuI GTATCC 1 cut(s) 239
BglI GCCNNNNNGGC 2 cut(s) 49, 598
BisI GCNGC 7 cut(s) 653, 807, 855, 858, 924, 987, 990
BlnI CCTAGG 1 cut(s) 476
BlpI GCTNAGC 1 cut(s) 39
BlsI GCNGC 7 cut(s) 654, 808, 856, 859, 925, 988, 991
Bme1390I CCNGG 4 cut(s) 49, 395, 692, 776
Bme18I GGWCC 2 cut(s) 644, 719
BmgT120I GGNCC 2 cut(s) 644, 719
BmiI GGNNCC 8 cut(s) 53, 103, 192, 300, 696, 762, 966, 1041
BmrFI CCNGG 4 cut(s) 49, 395, 692, 776
BmrI ACTGGG 1 cut(s) 621
BmsI GCATC 2 cut(s) 399, 883
BmtI GCTAGC 1 cut(s) 96
BmuI ACTGGG 1 cut(s) 621
BpmI CTGGAG 1 cut(s) 88
Bpu10I CCTNAGC 1 cut(s) 783
Bpu1102I GCTNAGC 1 cut(s) 39
BsaBI GATNNNNATC 1 cut(s) 413
BsaI GGTCTC 2 cut(s) 104, 171
BsaJI CCNNGG 3 cut(s) 476, 801, 1035
BsaXI ACNNNNNCTCC 6 cut(s) 345, 375, 921, 943, 951, 973
Bsc4I CCNNNNNNNGG 6 cut(s) 536, 709, 716, 903, 974, 975
Bse118I RCCGGY 7 cut(s) 18, 259, 488, 529, 680, 709, 716
Bse1I ACTGG 3 cut(s) 127, 616, 722
Bse8I GATNNNNATC 1 cut(s) 413
BseBI CCWGG 4 cut(s) 49, 395, 692, 776
BseDI CCNNGG 3 cut(s) 476, 801, 1035
BseGI GGATG 2 cut(s) 414, 762
BseJI GATNNNNATC 1 cut(s) 413
BseLI CCNNNNNNNGG 6 cut(s) 536, 709, 716, 903, 974, 975
BseMII CTCAG 2 cut(s) 383, 797
BseNI ACTGG 3 cut(s) 127, 616, 722
BseSI GKGCMC 1 cut(s) 969
BseXI GCAGC 7 cut(s) 664, 818, 841, 869, 935, 973, 976
Bsh1236I CGCG 1 cut(s) 30
Bsh1285I CGRYCG 3 cut(s) 19, 681, 717
BshFI GGCC 1 cut(s) 203
BshNI GGYRCC 3 cut(s) 760, 964, 1039
BshTI ACCGGT 3 cut(s) 259, 680, 716
BsiEI CGRYCG 3 cut(s) 19, 681, 717
BslI CCNNNNNNNGG 6 cut(s) 536, 709, 716, 903, 974, 975
BsmAI GTCTC 5 cut(s) 104, 171, 372, 549, 973
BsmBI CGTCTC 1 cut(s) 549
BsnI GGCC 1 cut(s) 203
Bso31I GGTCTC 2 cut(s) 104, 171
Bsp1286I GDGCHC 1 cut(s) 969
Bsp1407I TGTACA 1 cut(s) 789
Bsp143I GATC 5 cut(s) 159, 332, 340, 390, 539
Bsp1720I GCTNAGC 1 cut(s) 39
Bsp19I CCATGG 1 cut(s) 1035
BspACI CCGC 8 cut(s) 28, 45, 90, 290, 359, 374, 699, 749
BspANI GGCC 1 cut(s) 203
BspCNI CTCAG 2 cut(s) 382, 796
BspFNI CGCG 1 cut(s) 30
BspLI GGNNCC 8 cut(s) 53, 103, 192, 300, 696, 762, 966, 1041
BspMAI CTGCAG 2 cut(s) 859, 925
BspMI ACCTGC 1 cut(s) 898
BspOI GCTAGC 1 cut(s) 96
BspPI GGATC 4 cut(s) 340, 348, 385, 547
BspT107I GGYRCC 3 cut(s) 760, 964, 1039
BspTNI GGTCTC 2 cut(s) 104, 171
BsrFI RCCGGY 7 cut(s) 18, 259, 488, 529, 680, 709, 716
BsrGI TGTACA 1 cut(s) 789
BsrI ACTGG 3 cut(s) 127, 616, 722
BssAI RCCGGY 7 cut(s) 18, 259, 488, 529, 680, 709, 716
BssECI CCNNGG 3 cut(s) 476, 801, 1035
BssMI GATC 5 cut(s) 159, 332, 340, 390, 539
BssNAI GTATAC 2 cut(s) 688, 913
BssSI CACGAG 1 cut(s) 428
BssT1I CCWWGG 2 cut(s) 476, 1035
Bst1107I GTATAC 2 cut(s) 688, 913
Bst2BI CACGAG 1 cut(s) 428
Bst2UI CCWGG 4 cut(s) 49, 395, 692, 776
Bst4CI ACNGT 2 cut(s) 298, 955
Bst6I CTCTTC 1 cut(s) 71
BstAPI GCANNNNNTGC 1 cut(s) 409
BstAUI TGTACA 1 cut(s) 789
BstC8I GCNNGC 4 cut(s) 45, 94, 816, 852
BstDEI CTNAG 3 cut(s) 39, 369, 783
BstDSI CCRYGG 2 cut(s) 801, 1035
BstF5I GGATG 2 cut(s) 414, 762
BstFNI CGCG 1 cut(s) 30
BstH2I RGCGCY 1 cut(s) 127
BstHHI GCGC 3 cut(s) 32, 126, 535
BstKTI GATC 5 cut(s) 162, 335, 343, 393, 542
BstMAI GTCTC 5 cut(s) 104, 171, 372, 549, 973
BstMBI GATC 5 cut(s) 159, 332, 340, 390, 539
BstMCI CGRYCG 3 cut(s) 19, 681, 717
BstMWI GCNNNNNNNGC 9 cut(s) 44, 49, 409, 598, 806, 815, 824, 833, 1046
BstNI CCWGG 4 cut(s) 49, 395, 692, 776
BstSCI CCNGG 4 cut(s) 47, 393, 690, 774
BstSFI CTRYAG 2 cut(s) 855, 921
BstSLI GKGCMC 1 cut(s) 969
BstUI CGCG 1 cut(s) 30
BstV1I GCAGC 7 cut(s) 664, 818, 841, 869, 935, 973, 976
BstX2I RGATCY 2 cut(s) 332, 390
BstYI RGATCY 2 cut(s) 332, 390
BstZ17I GTATAC 2 cut(s) 688, 913
BsuI GTATCC 1 cut(s) 239
BsuRI GGCC 1 cut(s) 203
BtgI CCRYGG 2 cut(s) 801, 1035
BtsCI GGATG 2 cut(s) 414, 762
BtsI GCAGTG 1 cut(s) 918
BtsIMutI CAGTG 3 cut(s) 134, 609, 918
BveI ACCTGC 1 cut(s) 898
Cac8I GCNNGC 4 cut(s) 45, 94, 816, 852
CfoI GCGC 3 cut(s) 32, 126, 535
Cfr10I RCCGGY 7 cut(s) 18, 259, 488, 529, 680, 709, 716
Cfr13I GGNCC 2 cut(s) 644, 719
CseI GACGC 3 cut(s) 149, 788, 1038
Csp6I GTAC 6 cut(s) 13, 25, 257, 746, 772, 790
CspAI ACCGGT 3 cut(s) 259, 680, 716
CviAII CATG 2 cut(s) 604, 1036
CviQI GTAC 6 cut(s) 13, 25, 257, 746, 772, 790
DdeI CTNAG 3 cut(s) 39, 369, 783
DpnI GATC 5 cut(s) 161, 334, 342, 392, 541
DpnII GATC 5 cut(s) 159, 332, 340, 390, 539
Eam1104I CTCTTC 1 cut(s) 71
EarI CTCTTC 1 cut(s) 71
EciI GGCGGA 2 cut(s) 279, 389
Eco130I CCWWGG 2 cut(s) 476, 1035
Eco31I GGTCTC 2 cut(s) 104, 171
Eco47I GGWCC 2 cut(s) 644, 719
Eco57I CTGAAG 1 cut(s) 18
EcoRI GAATTC 1 cut(s) 148
EcoRII CCWGG 4 cut(s) 47, 393, 690, 774
EcoT14I CCWWGG 2 cut(s) 476, 1035
ErhI CCWWGG 2 cut(s) 476, 1035
Esp3I CGTCTC 1 cut(s) 549
FaeI CATG 2 cut(s) 607, 1039
FatI CATG 2 cut(s) 603, 1035
FauI CCCGC 2 cut(s) 52, 706
FblI GTMKAC 3 cut(s) 138, 687, 912
Fnu4HI GCNGC 7 cut(s) 653, 807, 855, 858, 924, 987, 990
FokI GGATG 2 cut(s) 421, 769
Fsp4HI GCNGC 7 cut(s) 653, 807, 855, 858, 924, 987, 990
FspBI CTAG 3 cut(s) 93, 477, 705
GlaI GCGC 3 cut(s) 31, 125, 534
GluI GCNGC 7 cut(s) 653, 807, 855, 858, 924, 987, 990
GsuI CTGGAG 1 cut(s) 88
HaeII RGCGCY 1 cut(s) 127
HaeIII GGCC 1 cut(s) 203
HgaI GACGC 3 cut(s) 149, 788, 1038
HhaI GCGC 3 cut(s) 32, 126, 535
Hin1II CATG 2 cut(s) 607, 1039
Hin6I GCGC 3 cut(s) 30, 124, 533
HinP1I GCGC 3 cut(s) 30, 124, 533
HincII GTYRAC 1 cut(s) 139
HindII GTYRAC 1 cut(s) 139
HinfI GANTC 6 cut(s) 57, 177, 234, 278, 379, 1003
HphI GGTGA 3 cut(s) 373, 440, 637
Hpy166II GTNNAC 5 cut(s) 139, 688, 772, 790, 913
Hpy188I TCNGA 8 cut(s) 56, 121, 159, 186, 233, 384, 678, 951
Hpy188III TCNNGA 9 cut(s) 74, 170, 275, 302, 428, 515, 642, 901, 1013
Hpy8I GTNNAC 5 cut(s) 139, 688, 772, 790, 913
Hpy99I CGWCG 6 cut(s) 140, 143, 190, 298, 464, 902
HpyAV CCTTC 1 cut(s) 863
HpyCH4III ACNGT 2 cut(s) 298, 955
HpyCH4V TGCA 5 cut(s) 403, 412, 857, 878, 923
HpyF10VI GCNNNNNNNGC 9 cut(s) 44, 49, 409, 598, 806, 815, 824, 833, 1046
HpyF3I CTNAG 3 cut(s) 39, 369, 783
Hsp92II CATG 2 cut(s) 607, 1039
HspAI GCGC 3 cut(s) 30, 124, 533
Kzo9I GATC 5 cut(s) 159, 332, 340, 390, 539
LmnI GCTCC 6 cut(s) 57, 107, 196, 649, 700, 964
Lsp1109I GCAGC 7 cut(s) 664, 818, 841, 869, 935, 973, 976
LweI GCATC 2 cut(s) 399, 883
MaeI CTAG 3 cut(s) 93, 477, 705
MaeIII GTNAC 5 cut(s) 196, 283, 425, 482, 625
MalI GATC 5 cut(s) 161, 334, 342, 392, 541
MboI GATC 5 cut(s) 159, 332, 340, 390, 539
MboII GAAGA 2 cut(s) 58, 933
MflI RGATCY 2 cut(s) 332, 390
MhlI GDGCHC 1 cut(s) 969
MluCI AATT 4 cut(s) 148, 269, 843, 1008
MlyI GAGTC 2 cut(s) 51, 272
MmeI TCCRAC 5 cut(s) 79, 209, 310, 602, 701
MnlI CCTC 9 cut(s) 81, 214, 378, 399, 591, 747, 760, 897, 923
MspR9I CCNGG 4 cut(s) 49, 395, 692, 776
MvaI CCWGG 4 cut(s) 49, 395, 692, 776
MvnI CGCG 1 cut(s) 30
MwoI GCNNNNNNNGC 9 cut(s) 44, 49, 409, 598, 806, 815, 824, 833, 1046
NcoI CCATGG 1 cut(s) 1035
NdeII GATC 5 cut(s) 159, 332, 340, 390, 539
NheI GCTAGC 1 cut(s) 92
NlaIII CATG 2 cut(s) 607, 1039
NlaIV GGNNCC 8 cut(s) 53, 103, 192, 300, 696, 762, 966, 1041
NmuCI GTSAC 4 cut(s) 196, 283, 425, 625
PcsI WCGNNNNNNNCGW 3 cut(s) 105, 168, 206
PfeI GAWTC 4 cut(s) 177, 234, 379, 1003
PflFI GACNNNGTC 2 cut(s) 115, 281
PfoI TCCNGGA 1 cut(s) 393
PinAI ACCGGT 3 cut(s) 259, 680, 716
PkrI GCNGC 7 cut(s) 654, 808, 856, 859, 925, 988, 991
PleI GAGTC 2 cut(s) 51, 272
PpsI GAGTC 2 cut(s) 51, 272
Psp6I CCWGG 4 cut(s) 47, 393, 690, 774
PspGI CCWGG 4 cut(s) 47, 393, 690, 774
PspN4I GGNNCC 8 cut(s) 53, 103, 192, 300, 696, 762, 966, 1041
PspPI GGNCC 2 cut(s) 644, 719
PstI CTGCAG 2 cut(s) 859, 925
PsuI RGATCY 2 cut(s) 332, 390
PsyI GACNNNGTC 2 cut(s) 115, 281
RsaI GTAC 6 cut(s) 14, 26, 258, 747, 773, 791
RsaNI GTAC 6 cut(s) 13, 25, 257, 746, 772, 790
SalI GTCGAC 1 cut(s) 137
SatI GCNGC 7 cut(s) 653, 807, 855, 858, 924, 987, 990
Sau3AI GATC 5 cut(s) 159, 332, 340, 390, 539
Sau96I GGNCC 2 cut(s) 644, 719
SchI GAGTC 2 cut(s) 51, 272
ScrFI CCNGG 4 cut(s) 49, 395, 692, 776
SduI GDGCHC 1 cut(s) 969
SfaNI GCATC 2 cut(s) 399, 883
SfcI CTRYAG 2 cut(s) 855, 921
SgrAI CRCCGGYG 1 cut(s) 488
SgrDI CGTCGACG 1 cut(s) 137
SinI GGWCC 2 cut(s) 644, 719
Sse9I AATT 4 cut(s) 148, 269, 843, 1008
SsiI CCGC 8 cut(s) 28, 45, 90, 290, 359, 374, 699, 749
SspMI CTAG 3 cut(s) 93, 477, 705
StyD4I CCNGG 4 cut(s) 47, 393, 690, 774
StyI CCWWGG 2 cut(s) 476, 1035
TaaI ACNGT 2 cut(s) 298, 955
TaqI TCGA 3 cut(s) 9, 138, 237
TaqII GACCGA 1 cut(s) 271
TasI AATT 4 cut(s) 148, 269, 843, 1008
TatI WGTACW 1 cut(s) 789
TfiI GAWTC 4 cut(s) 177, 234, 379, 1003
TscAI CASTG 3 cut(s) 134, 616, 925
TseFI GTSAC 4 cut(s) 196, 283, 425, 625
TseI GCWGC 7 cut(s) 652, 806, 854, 857, 923, 986, 989
Tsp45I GTSAC 4 cut(s) 196, 283, 425, 625
TspDTI ATGAA 3 cut(s) 620, 655, 933
TspRI CASTG 3 cut(s) 134, 616, 925
Tth111I GACNNNGTC 2 cut(s) 115, 281
VpaK11BI GGWCC 2 cut(s) 644, 719
XapI RAATTY 4 cut(s) 148, 269, 843, 1008
XmaJI CCTAGG 1 cut(s) 476
XmiI GTMKAC 3 cut(s) 138, 687, 912
XspI CTAG 3 cut(s) 93, 477, 705
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.