Rorug07G0279600

Glycine-rich domain-containing protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0002521
Physical Location & Seq
Forward (+)
26923432 .. 26927682
4251 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0279600.1

Sequence Viewer

Length: 516 bp
ATGGGGACGAGGCAAAGGGACTTGAACAAGTCCTTCAACCTCGCCATACGTTCTCTGCTCGCTCCCTGCTCTTTTCAGGAATTCTGTGAAGCGCTGCCGAGTTTCACAGCTGCTGAGCATGAACGTCTTCACAGGCTCTTTACTCAGGTCATGAGTTCTTTGCATGGGAATGTAGAGGACGAGTTTCAGCATATATGTCTCGAAACACAGGTTGGAACTGCAATGGATACTGTAGAGCAACTGGTGGAAGAACAAAGTCTGGATCCTTTGTTTTCAAATAAGACTAATGTAATGGATGTTGCTCACGATCTGTCAAGAACAACAAAGAAGGAGATCCAGTACTTGACAGAGATGCTTGAAAAGGCGGAGGCACAGAATCACCTTAAGCGGGATCGTATTGAACTGTTAAAGAAAGGAAGACCAGATATGTCGGACATGGCAAATGCTGTTGAGAAGTTGAAAAGTGGGATTTCAAGTTATGGAATGTATAACAGCAATGGGGTCCATGATTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRDP-like

Protein Analysis

171

Amino Acids

19.44

Weight (kDa)

5.48

Isoelectric Point (pI)

38.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nnf1 PF03980 43 - 136 1.5e-08 Nnf1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 365, 388
AclWI GGATC 4 cut(s) 257, 270, 328, 399
AcsI RAATTY 1 cut(s) 80
AfaI GTAC 1 cut(s) 341
AfeI AGCGCT 1 cut(s) 93
AfiI CCNNNNNNNGG 1 cut(s) 388
AflII CTTAAG 1 cut(s) 383
AgsI TTSAA 7 cut(s) 25, 37, 276, 359, 401, 460, 474
AjuI GAANNNNNNNTTGG 2 cut(s) 195, 227
AluBI AGCT 1 cut(s) 110
AluI AGCT 1 cut(s) 110
Alw26I GTCTC 1 cut(s) 203
AlwI GGATC 4 cut(s) 257, 270, 328, 399
AlwNI CAGNNNCTG 1 cut(s) 113
Aor51HI AGCGCT 1 cut(s) 93
ApeKI GCWGC 2 cut(s) 94, 110
ApoI RAATTY 1 cut(s) 80
Asp700I GAANNNNTTC 1 cut(s) 126
AspLEI GCGC 1 cut(s) 94
AspS9I GGNCC 1 cut(s) 502
AsuHPI GGTGA 1 cut(s) 371
AvaII GGWCC 1 cut(s) 502
BamHI GGATCC 1 cut(s) 262
BbsI GAAGAC 2 cut(s) 119, 424
BbvI GCAGC 2 cut(s) 81, 97
BciVI GTATCC 1 cut(s) 220
BcoDI GTCTC 1 cut(s) 203
BfmI CTRYAG 1 cut(s) 231
BfoI RGCGCY 1 cut(s) 95
BfrI CTTAAG 1 cut(s) 383
BfuI GTATCC 1 cut(s) 220
BisI GCNGC 2 cut(s) 95, 111
BlpI GCTNAGC 1 cut(s) 114
BlsI GCNGC 2 cut(s) 96, 112
BmcAI AGTACT 1 cut(s) 341
Bme18I GGWCC 1 cut(s) 502
BmgT120I GGNCC 1 cut(s) 502
BmiI GGNNCC 2 cut(s) 264, 503
BmsI GCATC 1 cut(s) 342
BpiI GAAGAC 2 cut(s) 119, 424
Bpu1102I GCTNAGC 1 cut(s) 114
BsaXI ACNNNNNCTCC 2 cut(s) 323, 353
Bsc4I CCNNNNNNNGG 1 cut(s) 388
Bse1I ACTGG 2 cut(s) 246, 337
Bse3DI GCAATG 2 cut(s) 228, 502
BseGI GGATG 1 cut(s) 301
BseLI CCNNNNNNNGG 1 cut(s) 388
BseMI GCAATG 2 cut(s) 228, 502
BseMII CTCAG 2 cut(s) 105, 158
BseNI ACTGG 2 cut(s) 246, 337
BseXI GCAGC 2 cut(s) 81, 97
BslFI GGGAC 2 cut(s) 19, 32
BslI CCNNNNNNNGG 1 cut(s) 388
BsmAI GTCTC 1 cut(s) 203
BsmFI GGGAC 2 cut(s) 19, 32
Bsp143I GATC 4 cut(s) 262, 307, 333, 391
Bsp1720I GCTNAGC 1 cut(s) 114
BspACI CCGC 2 cut(s) 365, 388
BspCNI CTCAG 2 cut(s) 106, 157
BspHI TCATGA 2 cut(s) 150, 512
BspLI GGNNCC 2 cut(s) 264, 503
BspPI GGATC 4 cut(s) 257, 270, 328, 399
BspTI CTTAAG 1 cut(s) 383
BsrDI GCAATG 2 cut(s) 228, 502
BsrI ACTGG 2 cut(s) 246, 337
BssMI GATC 4 cut(s) 262, 307, 333, 391
Bst4CI ACNGT 2 cut(s) 232, 405
BstAFI CTTAAG 1 cut(s) 383
BstC8I GCNNGC 1 cut(s) 60
BstDEI CTNAG 2 cut(s) 114, 144
BstF5I GGATG 1 cut(s) 301
BstH2I RGCGCY 1 cut(s) 95
BstHHI GCGC 1 cut(s) 94
BstKTI GATC 4 cut(s) 265, 310, 336, 394
BstMAI GTCTC 1 cut(s) 203
BstMBI GATC 4 cut(s) 262, 307, 333, 391
BstSFI CTRYAG 1 cut(s) 231
BstV1I GCAGC 2 cut(s) 81, 97
BstV2I GAAGAC 2 cut(s) 119, 424
BstX2I RGATCY 2 cut(s) 262, 333
BstYI RGATCY 2 cut(s) 262, 333
BsuI GTATCC 1 cut(s) 220
BtsCI GGATG 1 cut(s) 301
Cac8I GCNNGC 1 cut(s) 60
CaiI CAGNNNCTG 1 cut(s) 113
CciI TCATGA 2 cut(s) 150, 512
CfoI GCGC 1 cut(s) 94
Cfr13I GGNCC 1 cut(s) 502
Csp6I GTAC 1 cut(s) 340
CviAII CATG 6 cut(s) 119, 151, 164, 436, 506, 513
CviJI RGCY 2 cut(s) 110, 136
CviKI_1 RGCY 2 cut(s) 110, 136
CviQI GTAC 1 cut(s) 340
DdeI CTNAG 2 cut(s) 114, 144
DpnI GATC 4 cut(s) 264, 309, 335, 393
DpnII GATC 4 cut(s) 262, 307, 333, 391
EciI GGCGGA 1 cut(s) 380
Eco47I GGWCC 1 cut(s) 502
Eco47III AGCGCT 1 cut(s) 93
EcoRI GAATTC 1 cut(s) 80
FaeI CATG 6 cut(s) 122, 154, 167, 439, 509, 516
FaqI GGGAC 2 cut(s) 19, 32
FatI CATG 6 cut(s) 118, 150, 163, 435, 505, 512
FauI CCCGC 1 cut(s) 381
Fnu4HI GCNGC 2 cut(s) 95, 111
FokI GGATG 1 cut(s) 308
Fsp4HI GCNGC 2 cut(s) 95, 111
GlaI GCGC 1 cut(s) 93
GluI GCNGC 2 cut(s) 95, 111
HaeII RGCGCY 1 cut(s) 95
HhaI GCGC 1 cut(s) 94
Hin1II CATG 6 cut(s) 122, 154, 167, 439, 509, 516
Hin6I GCGC 1 cut(s) 92
HinP1I GCGC 1 cut(s) 92
HinfI GANTC 2 cut(s) 376, 509
HphI GGTGA 1 cut(s) 371
Hpy188I TCNGA 1 cut(s) 433
Hpy188III TCNNGA 7 cut(s) 77, 151, 200, 260, 305, 315, 513
HpyAV CCTTC 2 cut(s) 43, 322
HpyCH4III ACNGT 2 cut(s) 232, 405
HpyCH4IV ACGT 2 cut(s) 49, 124
HpyCH4V TGCA 2 cut(s) 163, 221
HpyF3I CTNAG 2 cut(s) 114, 144
HpySE526I ACGT 2 cut(s) 49, 124
Hsp92II CATG 6 cut(s) 122, 154, 167, 439, 509, 516
HspAI GCGC 1 cut(s) 92
Kzo9I GATC 4 cut(s) 262, 307, 333, 391
LmnI GCTCC 1 cut(s) 67
LpnPI CCDG 9 cut(s) 62, 79, 118, 131, 194, 227, 245, 350, 435
Lsp1109I GCAGC 2 cut(s) 81, 97
LweI GCATC 1 cut(s) 342
MaeII ACGT 2 cut(s) 49, 124
MalI GATC 4 cut(s) 264, 309, 335, 393
MboI GATC 4 cut(s) 262, 307, 333, 391
MboII GAAGA 3 cut(s) 119, 260, 429
MflI RGATCY 2 cut(s) 262, 333
MluCI AATT 1 cut(s) 80
MmeI TCCRAC 2 cut(s) 193, 411
MnlI CCTC 4 cut(s) 3, 50, 169, 361
MroXI GAANNNNTTC 1 cut(s) 126
MseI TTAA 2 cut(s) 384, 407
MslI CAYNNNNRTG 1 cut(s) 168
MspA1I CMGCKG 1 cut(s) 110
MspCI CTTAAG 1 cut(s) 383
NdeII GATC 4 cut(s) 262, 307, 333, 391
NlaIII CATG 6 cut(s) 122, 154, 167, 439, 509, 516
NlaIV GGNNCC 2 cut(s) 264, 503
NmeAIII GCCGAG 1 cut(s) 123
PagI TCATGA 2 cut(s) 150, 512
PdmI GAANNNNTTC 1 cut(s) 126
PfeI GAWTC 2 cut(s) 376, 509
PkrI GCNGC 2 cut(s) 96, 112
PspN4I GGNNCC 2 cut(s) 264, 503
PspPI GGNCC 1 cut(s) 502
PstNI CAGNNNCTG 1 cut(s) 113
PsuI RGATCY 2 cut(s) 262, 333
PvuII CAGCTG 1 cut(s) 110
RsaI GTAC 1 cut(s) 341
RsaNI GTAC 1 cut(s) 340
RseI CAYNNNNRTG 1 cut(s) 168
SaqAI TTAA 2 cut(s) 384, 407
SatI GCNGC 2 cut(s) 95, 111
Sau3AI GATC 4 cut(s) 262, 307, 333, 391
Sau96I GGNCC 1 cut(s) 502
ScaI AGTACT 1 cut(s) 341
SetI ASST 7 cut(s) 42, 52, 112, 127, 150, 213, 384
SfaNI GCATC 1 cut(s) 342
SfcI CTRYAG 1 cut(s) 231
SinI GGWCC 1 cut(s) 502
SmiMI CAYNNNNRTG 1 cut(s) 168
SmlI CTYRAG 1 cut(s) 383
SmoI CTYRAG 1 cut(s) 383
Sse9I AATT 1 cut(s) 80
SsiI CCGC 2 cut(s) 365, 388
TaaI ACNGT 2 cut(s) 232, 405
TaiI ACGT 2 cut(s) 52, 127
TaqI TCGA 1 cut(s) 201
TasI AATT 1 cut(s) 80
TatI WGTACW 1 cut(s) 339
TfiI GAWTC 2 cut(s) 376, 509
Tru1I TTAA 2 cut(s) 384, 407
Tru9I TTAA 2 cut(s) 384, 407
TseI GCWGC 2 cut(s) 94, 110
TspDTI ATGAA 2 cut(s) 135, 501
Vha464I CTTAAG 1 cut(s) 383
VpaK11BI GGWCC 1 cut(s) 502
XapI RAATTY 1 cut(s) 80
XmnI GAANNNNTTC 1 cut(s) 126
ZrmI AGTACT 1 cut(s) 341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.