Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2A

Orthogroup ID

OG0002521

Physical Location & Seq

Genomic Coordinates Reverse (-)

22074704 .. 22080129

Gene Structure 5426 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2AG216500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2727 bp

ATGGATGATGGTAGAGGAAGTGTGCGTGAGGAACCAGGAAGAGTCGTACTTTGTACATTGTCAAGATCCCACGCTATAAAATATCTGACTGAAACTCGTAAGAAAGCAGAAGCTTCAATCAGTCTCGGACCCCACGTTAACCGTCGTCGGAACTATTTTCAGATGGAGATGGAGATGGAGATGGAGAAGGAACAGGAGCTTGAGTGGACCAAAGCGCAGAGCATTGGAATAAGCGTAGACCTTGTGGCTGCAGCAAAAAAGCAGCTTCAGTTTCTTGCTGCTGTTGATAGGAATCGGTATCTGTACGAAGGGCTGGCTCTTAACAGGGCTATCTATAGGTACAACTCTTGTTGGCTTCCATTGCTTGCCAAACATTCAGACTCCCAGGTTTCTGAGGGACCTTTGGTTGTACCTCTTGATTGTGAATGGATTTGGCATTGTCACAGGCTAAATCCGGTTCGATACAAGACTGACTGTGAAGAACTTTATGGAAAGATTCTTGACAACTCGAATGTTGTTTCCTCTGTTGAAGGTTCTTGTAAAAGTAAAACTGAAGAAATTTGGAATAGTCTGTATCCAGCAGAACCCTACAACTTCAACTTACAAAAGGCATTGTCCGAGGATATATCTGAAAGGATTTCCAAACTTGACAAATGCACCAAATATGATCTGGTTTCAGCTGTTAAAAGACAATCTCCTTTCTTTTACCAGGTATCTCCACCCCATATGAACCATGATCTGTTTCTTGAAGGAGCTGCGTCTAGATATAAAGGGTTTCTTCAGTTAATCAAGAGCAATAATGAGAAGTCTCTACGACGTTTCTGCGTTCCAACATATGATATTGATCTTATCTGGCATACTCACCAATTACATCCTGTATCCTATTGTAAAGATCTGCATGAACTCCTTGGAAAGGTATTGGAGCATGACGACATGGATTCGGACAGAACTAAAGGGAAGAAGCTGGATACTGGGTTCTCTGTAACCACCAAGCAATGGGAAGAGGCATTTGGTACCAGGTATTGGAGAGCAGGGGCAATGTATAGAGGCAGGGCTCCATCTCCTCTCACAACCACTCCTTATCAGTCTAACATCACTAGCAAGGATGTAATTGCATGTCCTGATCATCAGAAAGTAATTGAGCTTCCAGAGGTGAAAGCTGTGGAGGTCCTTTTGGAGTTTCTGGAAGTTAGGAACTTACCAGAAGGACACAAAGGGCTTCTCTTTGCCTCATTCAGTAAGACTACAAAGGATATATTTTTCAATGCTAAGCGGCGACTCAGCATTTTTTCTGAGTTTGGAGAGAAACAGGTTGCTTCTTTCCAGTGTGAACCTACTGGAGAGCTGCTTTTTGAACTCATGTCCCATTCACCTTTATATATACCAATGAAAAGGGCATATAAAACATTAGGTTCTGCTTCGTTCTCTCTTCAAGACTGTCTAGTCCCACCCTCTAAATTATATGCAGAAAAATGGTTAGAGATGGTGCCAAGCTCTAAAGTTGGGAACTTACAACCAATCTACATGCGATTCGCTATGTCTTTTACTATTCCAACCATAGCACAACACACACTTCATGTGGTTCGTTCTCGACCGTTTTCAAAAAGTTCTTGCTTTTTCCCCTTTGCTGGGAAGAATCAAGATGCCAAGAATTGGACACAAGTGATTGATGAAACTGGAACAGAGGTCCTCCGCCTGCAAATGAGGGATACTGAAAAGGAAAAGGTAAAGGGAATCAGCGTTCCAAGGAAAGAGGTGGTTGGAATTACCAAGTCTGGGAAAATATGTACTCTTGCAGAATTTGTGGGGACTGGTTGGTCTTTGATAGATTCACACTGGTCCCTTCATCGTGAGAAAAATTCCGAGGGATACCTCTTTTTGCTAAAGGGAAAAAAGATGGTCAAATTCTTCCCAGGCAGAAAGCTGGATTATGAACATAAGCATTATGAGGAAAATACAACTGAAGATAAAACTGAACAACACTTCGTGACCTTAGTTGAATTCTCTGCTGAAGATCCTTATGGCAAAGCAGTGGCATTGCTTGACCTAAAATCCGGGTGCATCAAGGTAAAGGAAGAGTCAATAACAGTTCCTGGGGTCATAGTGGCTTTTATACTGTCGGATAAGCTAAAGAAGGAAAGATATGACGGGTTTGCTGTTAACCCTGCGGAGATGGGTAGTGTCGAGGAAGAAATTAAAGCGAACCCTGAGGAAGGCAAAGGCACCAACTTGAGCTCTTCTGGAGCAAGTGTAGTTAACCTTAAATCTGAGGTGGTTGAAGGAAATGTAGTGACGTCTCAAAAAGGTGGAGGCTGTGGTGGAGCATGTGGCAGTGGGTGTGGAAATGCAACAAGGAGTGCCGGCGCTGGTGGATGTGGAAGTGGTTGTGGTGGAGGATGCGGAAACATGGAGAAGAGTAGCGCTTGCGGCAGTGGATGTGGTGGAGGATGTGGAAACTTGGTGAAGAGTGGCGGCTGTGGTGGTGGCTGTGGAGGTGGCTGTGGTGGTGGCTGTGGAGGTGGTTGTGGAAACATACTGAAGAGTGGTGGCTGTGGTGGCTGTGGCGGCGGCTGTGGGAACATACTGAAGAGTGGTGGCTGCGGTGGGTGTGGTGGTAGCGGTGGTTGTGGTAGTGGGTGCGGAGGCTTATTCAAGGGTAATGGTTATCATGAAAATACATCTGGTAACTCATGTGCCGAAGACCACCCAAAGGCAGTAGTTGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006970 GO:0008150 GO:0009628 GO:0009787 GO:0009966 GO:0009987 GO:0010646 GO:0010928 GO:0016020 GO:0023051 GO:0033554 GO:0044464 GO:0048580 GO:0048583 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051716 GO:0065007 GO:0071214 GO:0071470 GO:0071944 GO:0104004 GO:1901419 GO:1905957 GO:2000026 GO:2000028 GO:2000241

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GRDP-like

Protein Analysis

908

Amino Acids

99.21

Weight (kDa)

8.09

Isoelectric Point (pI)

44.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GRDP-like	PF07173	51 - 158	1.3e-10	Glycine-rich domain-containing protein-like
GRDP-like	PF07173	154 - 298	8e-58	Glycine-rich domain-containing protein-like
C2_GRDP	PF25334	385 - 517	1.2e-53	C2 domain of glycine-rich domain-containing protein
GRDP_C	PF25335	555 - 690	1.8e-36	Glycine Rich Domain Protein C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002521)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G22660 AT2G22660 AT2G22660 AT4G37900 AT4G37900
fragaria_vesca	FvH4_1g18970 FvH4_1g18970 FvH4_1g18970 FvH4_5g33890 FvH4_5g33890 FvH4_5g33890
malus_domestica	MD01G1044900.v1.1 MD08G1208800.v1.1 MD08G1209100.v1.1
prunus_persica	Prupe.1G539900_v2.0.a1 Prupe.6G209600_v2.0.a1
pyrus_communis	pycom08g18020
rosa_chinensis	RchiOBHm_Chr2g0109991 RchiOBHm_Chr7g0233251 RchiOBHm_Chr7g0233261
rosa_laevigata	RLG00000001282 RLG00000017776
rosa_multiflora	Rmu_co8210630.1_g000001 Rmu_sc0004650.1_g000001 Rmu_sc0008314.1_g000009 Rmu_sc0035251.1_g000001 Rmu_sc0035252.1_g000001
rosa_roxburghii	Rroxscaffold_2G00133800
rosa_rugosa	Rorug02G0165000 Rorug07G0279600
rosa_samantha	Rh2AG216500 Rh2BG226400 Rh2CG218700 Rh2DG222100 Rh7AG435600 Rh7AG435700 Rh7BG408000 Rh7CG454700 Rh7CG454800 Rh7DG423900
rosa_wichuraiana	Rw2G016730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	1442, 1816
AatII	GACGTC	1 cut(s)	2297
Acc65I	GGTACC	1 cut(s)	1013
AccB1I	GGYRCC	3 cut(s)	1013, 1486, 2222
AccB7I	CCANNNNNTGG	3 cut(s)	996, 1023, 1653
AccI	GTMKAC	1 cut(s)	237
AclWI	GGATC	2 cut(s)	60, 2009
AcsI	RAATTY	5 cut(s)	558, 1799, 1858, 1904, 2000
AcuI	CTGAAG	7 cut(s)	251, 573, 764, 1983, 2031, 2558, 2606
AcyI	GRCGYC	1 cut(s)	2294
AdeI	CACNNNGTG	1 cut(s)	1987
AfaI	GTAC	7 cut(s)	48, 55, 305, 341, 411, 1015, 1789
AfeI	AGCGCT	1 cut(s)	2422
AfiI	CCNNNNNNNGG	9 cut(s)	913, 996, 1023, 1628, 1629, 1653, 1776, 2213, 2710
AjnI	CCWGG	6 cut(s)	34, 384, 708, 1016, 1912, 2092
AjuI	GAANNNNNNNTTGG	2 cut(s)	993, 1025
AloI	GAACNNNNNNTCC	4 cut(s)	959, 991, 1725, 1757
Alw21I	GWGCWC	1 cut(s)	2237
Alw26I	GTCTC	3 cut(s)	128, 813, 2301
AlwI	GGATC	2 cut(s)	60, 2009
AlwNI	CAGNNNCTG	1 cut(s)	2093
Aor51HI	AGCGCT	1 cut(s)	2422
ApeKI	GCWGC	7 cut(s)	248, 251, 262, 278, 755, 1345, 2598
ApoI	RAATTY	5 cut(s)	558, 1799, 1858, 1904, 2000
Asp718I	GGTACC	1 cut(s)	1013
AspLEI	GCGC	3 cut(s)	217, 2366, 2423
AspS9I	GGNCC	6 cut(s)	128, 207, 398, 1168, 1687, 1839
AsuC2I	CCSGG	1 cut(s)	2056
AsuHPI	GGTGA	4 cut(s)	854, 1165, 1362, 2473
AvaII	GGWCC	6 cut(s)	128, 207, 398, 1168, 1687, 1839
AxyI	CCTNAGG	1 cut(s)	2208
BanI	GGYRCC	3 cut(s)	1013, 1486, 2222
BanII	GRGCYC	2 cut(s)	1057, 2237
BarI	GAAGNNNNNNTAC	2 cut(s)	1128, 1160
BbsI	GAAGAC	1 cut(s)	2706
Bbv12I	GWGCWC	1 cut(s)	2237
BbvI	GCAGC	7 cut(s)	235, 263, 265, 274, 742, 1332, 2585
BccI	CCATC	9 cut(s)	2, 157, 163, 169, 175, 1066, 1477, 1891, 2167
BcgI	CGANNNNNNTGC	2 cut(s)	804, 838
BciT130I	CCWGG	6 cut(s)	36, 386, 710, 1018, 1914, 2094
BciVI	GTATCC	5 cut(s)	585, 889, 961, 1702, 1862
BclI	TGATCA	1 cut(s)	1123
BcnI	CCSGG	1 cut(s)	2056
BcoDI	GTCTC	3 cut(s)	128, 813, 2301
BfaI	CTAG	3 cut(s)	762, 1098, 1442
BfmI	CTRYAG	2 cut(s)	249, 334
BfoI	RGCGCY	2 cut(s)	2367, 2424
BfuI	GTATCC	5 cut(s)	585, 889, 961, 1702, 1862
BglII	AGATCT	1 cut(s)	892
BlpI	GCTNAGC	1 cut(s)	1269
Bme1390I	CCNGG	7 cut(s)	36, 386, 710, 1018, 1914, 2056, 2094
Bme18I	GGWCC	6 cut(s)	128, 207, 398, 1168, 1687, 1839
BmgT120I	GGNCC	6 cut(s)	128, 207, 398, 1168, 1687, 1839
BmiI	GGNNCC	8 cut(s)	33, 130, 399, 1015, 1056, 1488, 1841, 2224
BmrFI	CCNGG	7 cut(s)	36, 386, 710, 1018, 1914, 2056, 2094
BmrI	ACTGGG	1 cut(s)	981
BmsI	GCATC	3 cut(s)	1633, 2070, 2387
BmuI	ACTGGG	1 cut(s)	981
BpiI	GAAGAC	1 cut(s)	2706
BpmI	CTGGAG	2 cut(s)	1359, 2262
Bpu1102I	GCTNAGC	1 cut(s)	1269
BpuEI	CTTGAG	2 cut(s)	221, 2251
BpuMI	CCSGG	1 cut(s)	2056
BsaBI	GATNNNNATC	2 cut(s)	291, 843
BsaHI	GRCGYC	1 cut(s)	2294
BsaJI	CCNNGG	7 cut(s)	384, 618, 907, 1745, 1863, 1912, 2093
BsaWI	WCCGGW	1 cut(s)	454
BsaXI	ACNNNNNCTCC	6 cut(s)	188, 218, 1060, 1090, 2235, 2265
Bsc4I	CCNNNNNNNGG	9 cut(s)	913, 996, 1023, 1628, 1629, 1653, 1776, 2213, 2710
Bse118I	RCCGGY	1 cut(s)	2360
Bse1I	ACTGG	6 cut(s)	976, 1324, 1342, 1681, 1816, 1841
Bse21I	CCTNAGG	1 cut(s)	2208
Bse3DI	GCAATG	4 cut(s)	359, 1001, 1044, 2036
Bse8I	GATNNNNATC	2 cut(s)	291, 843
BseBI	CCWGG	6 cut(s)	36, 386, 710, 1018, 1914, 2094
BseDI	CCNNGG	7 cut(s)	384, 618, 907, 1745, 1863, 1912, 2093
BseGI	GGATG	7 cut(s)	10, 871, 1111, 2378, 2402, 2441, 2453
BseJI	GATNNNNATC	2 cut(s)	291, 843
BseLI	CCNNNNNNNGG	9 cut(s)	913, 996, 1023, 1628, 1629, 1653, 1776, 2213, 2710
BseMI	GCAATG	4 cut(s)	359, 1001, 1044, 2036
BseMII	CTCAG	5 cut(s)	384, 1284, 1294, 2199, 2259
BseNI	ACTGG	6 cut(s)	976, 1324, 1342, 1681, 1816, 1841
BseRI	GAGGAG	1 cut(s)	1053
BseXI	GCAGC	7 cut(s)	235, 263, 265, 274, 742, 1332, 2585
BseYI	CCCAGC	1 cut(s)	1628
Bsh1285I	CGRYCG	1 cut(s)	1595
BshNI	GGYRCC	3 cut(s)	1013, 1486, 2222
BsiEI	CGRYCG	1 cut(s)	1595
BsiHKAI	GWGCWC	1 cut(s)	2237
BsiSI	CCGG	3 cut(s)	455, 2055, 2361
BslFI	GGGAC	5 cut(s)	411, 1348, 1430, 1822, 1825
BslI	CCNNNNNNNGG	9 cut(s)	913, 996, 1023, 1628, 1629, 1653, 1776, 2213, 2710
BsmAI	GTCTC	3 cut(s)	128, 813, 2301
BsmBI	CGTCTC	1 cut(s)	2301
BsmFI	GGGAC	5 cut(s)	411, 1348, 1430, 1822, 1825
Bsp1286I	GDGCHC	2 cut(s)	1057, 2237
Bsp1407I	TGTACA	1 cut(s)	53
Bsp143I	GATC	7 cut(s)	65, 667, 736, 844, 892, 1123, 2014
Bsp1720I	GCTNAGC	1 cut(s)	1269
BspCNI	CTCAG	5 cut(s)	385, 1285, 1293, 2200, 2260
BspHI	TCATGA	1 cut(s)	2668
BspLI	GGNNCC	8 cut(s)	33, 130, 399, 1015, 1056, 1488, 1841, 2224
BspMAI	CTGCAG	1 cut(s)	253
BspPI	GGATC	2 cut(s)	60, 2009
BspQI	GCTCTTC	1 cut(s)	2242
BspT107I	GGYRCC	3 cut(s)	1013, 1486, 2222
BsrDI	GCAATG	4 cut(s)	359, 1001, 1044, 2036
BsrFI	RCCGGY	1 cut(s)	2360
BsrGI	TGTACA	1 cut(s)	53
BsrI	ACTGG	6 cut(s)	976, 1324, 1342, 1681, 1816, 1841
BssAI	RCCGGY	1 cut(s)	2360
BssECI	CCNNGG	7 cut(s)	384, 618, 907, 1745, 1863, 1912, 2093
BssMI	GATC	7 cut(s)	65, 667, 736, 844, 892, 1123, 2014
BssNI	GRCGYC	1 cut(s)	2294
BssT1I	CCWWGG	2 cut(s)	907, 1745
Bst2UI	CCWGG	6 cut(s)	36, 386, 710, 1018, 1914, 2094
Bst4CI	ACNGT	6 cut(s)	143, 476, 1439, 1596, 2089, 2118
Bst6I	CTCTTC	9 cut(s)	34, 996, 1434, 2070, 2242, 2408, 2459, 2534, 2582
BstACI	GRCGYC	1 cut(s)	2294
BstAUI	TGTACA	1 cut(s)	53
BstC8I	GCNNGC	5 cut(s)	315, 366, 1697, 2362, 2425
BstDEI	CTNAG	7 cut(s)	393, 1269, 1280, 1293, 1993, 2208, 2268
BstENI	CCTNNNNNAGG	2 cut(s)	911, 2211
BstF5I	GGATG	7 cut(s)	10, 871, 1111, 2378, 2402, 2441, 2453
BstH2I	RGCGCY	2 cut(s)	2367, 2424
BstHHI	GCGC	3 cut(s)	217, 2366, 2423
BstKTI	GATC	7 cut(s)	68, 670, 739, 847, 895, 1126, 2017
BstMAI	GTCTC	3 cut(s)	128, 813, 2301
BstMBI	GATC	7 cut(s)	65, 667, 736, 844, 892, 1123, 2014
BstMCI	CGRYCG	1 cut(s)	1595
BstMWI	GCNNNNNNNGC	4 cut(s)	361, 2427, 2556, 2565
BstNI	CCWGG	6 cut(s)	36, 386, 710, 1018, 1914, 2094
BstNSI	RCATGY	3 cut(s)	1119, 1528, 2328
BstSCI	CCNGG	7 cut(s)	34, 384, 708, 1016, 1912, 2054, 2092
BstSFI	CTRYAG	2 cut(s)	249, 334
BstV1I	GCAGC	7 cut(s)	235, 263, 265, 274, 742, 1332, 2585
BstV2I	GAAGAC	1 cut(s)	2706
BstX2I	RGATCY	3 cut(s)	65, 892, 2014
BstYI	RGATCY	3 cut(s)	65, 892, 2014
Bsu36I	CCTNAGG	1 cut(s)	2208
BsuI	GTATCC	5 cut(s)	585, 889, 961, 1702, 1862
BtsCI	GGATG	7 cut(s)	10, 871, 1111, 2378, 2402, 2441, 2453
BtsI	GCAGTG	3 cut(s)	2037, 2338, 2437
BtsIMutI	CAGTG	5 cut(s)	1331, 1834, 2037, 2338, 2437
Cac8I	GCNNGC	5 cut(s)	315, 366, 1697, 2362, 2425
CaiI	CAGNNNCTG	1 cut(s)	2093
CciI	TCATGA	1 cut(s)	2668
CfoI	GCGC	3 cut(s)	217, 2366, 2423
Cfr10I	RCCGGY	1 cut(s)	2360
Cfr13I	GGNCC	6 cut(s)	128, 207, 398, 1168, 1687, 1839
CseI	GACGC	1 cut(s)	747
CsiI	ACCWGGT	2 cut(s)	708, 1016
Csp6I	GTAC	7 cut(s)	47, 54, 304, 340, 410, 1014, 1788
CviQI	GTAC	7 cut(s)	47, 54, 304, 340, 410, 1014, 1788
DdeI	CTNAG	7 cut(s)	393, 1269, 1280, 1293, 1993, 2208, 2268
DpnI	GATC	7 cut(s)	67, 669, 738, 846, 894, 1125, 2016
DpnII	GATC	7 cut(s)	65, 667, 736, 844, 892, 1123, 2014
DraIII	CACNNNGTG	1 cut(s)	1987
DrdI	GACNNNNNNGTC	2 cut(s)	1442, 1816
DseDI	GACNNNNNNGTC	2 cut(s)	1442, 1816
Eam1104I	CTCTTC	9 cut(s)	34, 996, 1434, 2070, 2242, 2408, 2459, 2534, 2582
EarI	CTCTTC	9 cut(s)	34, 996, 1434, 2070, 2242, 2408, 2459, 2534, 2582
EciI	GGCGGA	1 cut(s)	1682
Ecl136II	GAGCTC	1 cut(s)	2235
Eco130I	CCWWGG	2 cut(s)	907, 1745
Eco24I	GRGCYC	2 cut(s)	1057, 2237
Eco47I	GGWCC	6 cut(s)	128, 207, 398, 1168, 1687, 1839
Eco47III	AGCGCT	1 cut(s)	2422
Eco53kI	GAGCTC	1 cut(s)	2235
Eco57I	CTGAAG	7 cut(s)	251, 573, 764, 1983, 2031, 2558, 2606
Eco81I	CCTNAGG	1 cut(s)	2208
EcoICRI	GAGCTC	1 cut(s)	2235
EcoNI	CCTNNNNNAGG	2 cut(s)	911, 2211
EcoO109I	RGGNCCY	3 cut(s)	398, 1168, 1687
EcoRI	GAATTC	1 cut(s)	2000
EcoRII	CCWGG	6 cut(s)	34, 384, 708, 1016, 1912, 2092
EcoT14I	CCWWGG	2 cut(s)	907, 1745
EcoT38I	GRGCYC	2 cut(s)	1057, 2237
ErhI	CCWWGG	2 cut(s)	907, 1745
Esp3I	CGTCTC	1 cut(s)	2301
FalI	AAGNNNNNCTT	2 cut(s)	762, 794
FaqI	GGGAC	5 cut(s)	411, 1348, 1430, 1822, 1825
FauNDI	CATATG	2 cut(s)	726, 835
FbaI	TGATCA	1 cut(s)	1123
FblI	GTMKAC	1 cut(s)	237
FokI	GGATG	7 cut(s)	17, 858, 1118, 2385, 2409, 2448, 2460
FriOI	GRGCYC	2 cut(s)	1057, 2237
FspBI	CTAG	3 cut(s)	762, 1098, 1442
GlaI	GCGC	3 cut(s)	216, 2365, 2422
GsaI	CCCAGC	1 cut(s)	1632
GsuI	CTGGAG	2 cut(s)	1359, 2262
HaeII	RGCGCY	2 cut(s)	2367, 2424
HapII	CCGG	3 cut(s)	455, 2055, 2361
HgaI	GACGC	1 cut(s)	747
HhaI	GCGC	3 cut(s)	217, 2366, 2423
Hin1I	GRCGYC	1 cut(s)	2294
Hin6I	GCGC	3 cut(s)	215, 2364, 2421
HinP1I	GCGC	3 cut(s)	215, 2364, 2421
HincII	GTYRAC	3 cut(s)	139, 2161, 2257
HindII	GTYRAC	3 cut(s)	139, 2161, 2257
HindIII	AAGCTT	1 cut(s)	111
HpaI	GTTAAC	3 cut(s)	139, 2161, 2257
HpaII	CCGG	3 cut(s)	455, 2055, 2361
HphI	GGTGA	4 cut(s)	854, 1165, 1362, 2473
Hpy166II	GTNNAC	6 cut(s)	139, 207, 238, 1331, 2161, 2257
Hpy8I	GTNNAC	6 cut(s)	139, 207, 238, 1331, 2161, 2257
Hpy99I	CGWCG	3 cut(s)	147, 150, 819
HpyAV	CCTTC	9 cut(s)	181, 302, 524, 743, 1199, 1853, 2128, 2207, 2273
HpyCH4III	ACNGT	6 cut(s)	143, 476, 1439, 1596, 2089, 2118
HpyCH4IV	ACGT	3 cut(s)	135, 817, 2294
HpyCH4V	TGCA	9 cut(s)	251, 657, 898, 1115, 1466, 1699, 1796, 2061, 2348
HpyF10VI	GCNNNNNNNGC	4 cut(s)	361, 2427, 2556, 2565
HpyF3I	CTNAG	7 cut(s)	393, 1269, 1280, 1293, 1993, 2208, 2268
HpySE526I	ACGT	3 cut(s)	135, 817, 2294
Hsp92I	GRCGYC	1 cut(s)	2294
HspAI	GCGC	3 cut(s)	215, 2364, 2421
KpnI	GGTACC	1 cut(s)	1017
KroI	GCCGGC	1 cut(s)	2360
KroNI	GCCGGC	1 cut(s)	2362
Ksp22I	TGATCA	1 cut(s)	1123
KspAI	GTTAAC	3 cut(s)	139, 2161, 2257
Kzo9I	GATC	7 cut(s)	65, 667, 736, 844, 892, 1123, 2014
LguI	GCTCTTC	1 cut(s)	2242
LmnI	GCTCC	6 cut(s)	196, 752, 922, 1060, 2243, 2321
Lsp1109I	GCAGC	7 cut(s)	235, 263, 265, 274, 742, 1332, 2585
LweI	GCATC	3 cut(s)	1633, 2070, 2387
MabI	ACCWGGT	2 cut(s)	708, 1016
MaeI	CTAG	3 cut(s)	762, 1098, 1442
MaeII	ACGT	3 cut(s)	135, 817, 2294
MaeIII	GTNAC	5 cut(s)	440, 982, 1987, 2290, 2684
MalI	GATC	7 cut(s)	67, 669, 738, 846, 894, 1125, 2016
MboI	GATC	7 cut(s)	65, 667, 736, 844, 892, 1123, 2014
MflI	RGATCY	3 cut(s)	65, 892, 2014
MhlI	GDGCHC	2 cut(s)	1057, 2237
MlyI	GAGTC	4 cut(s)	51, 374, 1272, 2087
MmeI	TCCRAC	5 cut(s)	128, 854, 1577, 1741, 2100
MroNI	GCCGGC	1 cut(s)	2360
MseI	TTAA	9 cut(s)	138, 321, 684, 785, 2160, 2196, 2256, 2262, 2725
MspA1I	CMGCKG	1 cut(s)	680
MspI	CCGG	3 cut(s)	455, 2055, 2361
MspR9I	CCNGG	7 cut(s)	36, 386, 710, 1018, 1914, 2056, 2094
MvaI	CCWGG	6 cut(s)	36, 386, 710, 1018, 1914, 2094
MwoI	GCNNNNNNNGC	4 cut(s)	361, 2427, 2556, 2565
NaeI	GCCGGC	1 cut(s)	2362
NciI	CCSGG	1 cut(s)	2056
NdeI	CATATG	2 cut(s)	726, 835
NdeII	GATC	7 cut(s)	65, 667, 736, 844, 892, 1123, 2014
NgoMIV	GCCGGC	1 cut(s)	2360
NlaIV	GGNNCC	8 cut(s)	33, 130, 399, 1015, 1056, 1488, 1841, 2224
NmuCI	GTSAC	3 cut(s)	440, 1987, 2290
NspI	RCATGY	3 cut(s)	1119, 1528, 2328
PagI	TCATGA	1 cut(s)	2668
PciSI	GCTCTTC	1 cut(s)	2242
PcsI	WCGNNNNNNNCGW	1 cut(s)	132
PdiI	GCCGGC	1 cut(s)	2362
PfeI	GAWTC	7 cut(s)	292, 496, 938, 1530, 1636, 1734, 1829
PflMI	CCANNNNNTGG	3 cut(s)	996, 1023, 1653
PleI	GAGTC	4 cut(s)	50, 374, 1272, 2086
PpsI	GAGTC	4 cut(s)	50, 374, 1272, 2086
PpuMI	RGGWCCY	3 cut(s)	398, 1168, 1687
Psp124BI	GAGCTC	1 cut(s)	2237
Psp5II	RGGWCCY	3 cut(s)	398, 1168, 1687
Psp6I	CCWGG	6 cut(s)	34, 384, 708, 1016, 1912, 2092
PspFI	CCCAGC	1 cut(s)	1628
PspGI	CCWGG	6 cut(s)	34, 384, 708, 1016, 1912, 2092
PspN4I	GGNNCC	8 cut(s)	33, 130, 399, 1015, 1056, 1488, 1841, 2224
PspPI	GGNCC	6 cut(s)	128, 207, 398, 1168, 1687, 1839
PspPPI	RGGWCCY	3 cut(s)	398, 1168, 1687
PstI	CTGCAG	1 cut(s)	253
PstNI	CAGNNNCTG	1 cut(s)	2093
PsuI	RGATCY	3 cut(s)	65, 892, 2014
PvuII	CAGCTG	1 cut(s)	680
RsaI	GTAC	7 cut(s)	48, 55, 305, 341, 411, 1015, 1789
RsaNI	GTAC	7 cut(s)	47, 54, 304, 340, 410, 1014, 1788
SacI	GAGCTC	1 cut(s)	2237
SapI	GCTCTTC	1 cut(s)	2242
SaqAI	TTAA	9 cut(s)	138, 321, 684, 785, 2160, 2196, 2256, 2262, 2725
Sau3AI	GATC	7 cut(s)	65, 667, 736, 844, 892, 1123, 2014
Sau96I	GGNCC	6 cut(s)	128, 207, 398, 1168, 1687, 1839
SchI	GAGTC	4 cut(s)	51, 374, 1272, 2087
ScrFI	CCNGG	7 cut(s)	36, 386, 710, 1018, 1914, 2056, 2094
SduI	GDGCHC	2 cut(s)	1057, 2237
SexAI	ACCWGGT	2 cut(s)	708, 1016
SfaNI	GCATC	3 cut(s)	1633, 2070, 2387
SfcI	CTRYAG	2 cut(s)	249, 334
SinI	GGWCC	6 cut(s)	128, 207, 398, 1168, 1687, 1839
SmlI	CTYRAG	2 cut(s)	200, 2230
SmoI	CTYRAG	2 cut(s)	200, 2230
SspMI	CTAG	3 cut(s)	762, 1098, 1442
SstI	GAGCTC	1 cut(s)	2237
StyD4I	CCNGG	7 cut(s)	34, 384, 708, 1016, 1912, 2054, 2092
StyI	CCWWGG	2 cut(s)	907, 1745
TaaI	ACNGT	6 cut(s)	143, 476, 1439, 1596, 2089, 2118
TaiI	ACGT	3 cut(s)	138, 820, 2297
TaqI	TCGA	4 cut(s)	460, 509, 1591, 2184
TatI	WGTACW	2 cut(s)	53, 1787
TauI	GCSGC	5 cut(s)	1276, 2430, 2475, 2568, 2571
TfiI	GAWTC	7 cut(s)	292, 496, 938, 1530, 1636, 1734, 1829
Tru1I	TTAA	9 cut(s)	138, 321, 684, 785, 2160, 2196, 2256, 2262, 2725
Tru9I	TTAA	9 cut(s)	138, 321, 684, 785, 2160, 2196, 2256, 2262, 2725
TscAI	CASTG	5 cut(s)	1331, 1841, 2037, 2338, 2437
TseFI	GTSAC	3 cut(s)	440, 1987, 2290
TseI	GCWGC	7 cut(s)	248, 251, 262, 278, 755, 1345, 2598
Tsp45I	GTSAC	3 cut(s)	440, 1987, 2290
TspDTI	ATGAA	8 cut(s)	743, 915, 1403, 1565, 1686, 1835, 1947, 2685
TspRI	CASTG	5 cut(s)	1331, 1841, 2037, 2338, 2437
Van91I	CCANNNNNTGG	3 cut(s)	996, 1023, 1653
VpaK11BI	GGWCC	6 cut(s)	128, 207, 398, 1168, 1687, 1839
XagI	CCTNNNNNAGG	2 cut(s)	911, 2211
XapI	RAATTY	5 cut(s)	558, 1799, 1858, 1904, 2000
XbaI	TCTAGA	1 cut(s)	761
XceI	RCATGY	3 cut(s)	1119, 1528, 2328
XcmI	CCANNNNNNNNNTGG	1 cut(s)	667
XmiI	GTMKAC	1 cut(s)	237
XspI	CTAG	3 cut(s)	762, 1098, 1442
ZraI	GACGTC	1 cut(s)	2295

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.