Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0002521

Physical Location & Seq

Genomic Coordinates Forward (+)

14441598 .. 14446972

Gene Structure 5375 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1044900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2802 bp

ATGTCATTAAAGCATACTTGTACGAATGATTTTCCTACGTTGTACTGCTACAACTCCCAAGCTCCCTCCTATATTTCTGAGCGATCGATATCCTTCACAGCTTGTAATGGGAAGGCAACCCGAAGCTTCTGGCAATCAGTAAAAGTGCGCGTTAACCATCCGTCATCGGAGAAAATTCAGGCGGCGCAGCCAGCTTTCCGGCGACCTGGTCAAACTTGGATTTCCAATCCATTTCGAGACGTTTTGAAGATGGAGAAGGAACAAGAGATTGAGTGGATCAAAGCTCAGAACATTGGAATAAGCGTAGACCTTGTTGCTGCAGCCAAACAGCAGCTTCAGTTTCTTGCTGCTGTTGATCGGAATCGATATCTCTATGAAGGGCCTGCTCTTGAAAGGGCAATCTATAGGTACAATGCTTGTTGGCTCCCGATGCTTGCCAAACATTCAGAGTCCCAGATTTCTGAAGGACCTTTGGTTGTACCTCTTGATTGTGAATGGATTTGGCATTGTCACAGGCTAAATCCAGTTCAATACAAGATCGACTGTGAGAAACTTTATGGAAAGATCCTTGACAATTCTAATGTTTTATCGTCTGTTCAAGACTCTTGTAAAAGGCAAACTGAAGAAACTTGGAATAGTCTGTATCCAGAAGAACCATACGACTTAGACTTAAAGAAGGTGTTCTCAGAGGATATATCTGAAAGATCTGCTCGACTTGAAAAAAGCACCAAATATGATCTGGTTTCAGCTGTTAAAAGACAGAGTCCTTTCTTTTACCAGGTATCCAGACCCCACATGAACCATGATCTATTTATCGAAGGGGCTGTGGCTAGATATAAAGGCTTTCTTCATCTAATCAAGAGAAACAGAGAGAAGTCTGTAAAACGTTTCTGTGTTCCGACATACGATGTTGACCTTATCTGGCATACTCACCAATTGCATCCTGTATCTTATTGTAAAGACCTGAATGAACTAATGGGCAAGGTATTGGAGCATGATGACATGGACTCTGACAGAACTAAAGGGAAGAAACTTGATGTTGGGTTTTCTGGAACTACCAAGCAATGGGAAGAGACCTTTGGTATCAGGTATTGGAAGGCAGGAGCAATGCATAGAGGTAGTGCTCCATGTCCTGTTATGACCACTCCTTACAAGTCTAGCATGATGAGCAAGGATGTAGCTGCATCCATCGAGTATCAGAAACTAATTCAGCTTCCAGAGGTTAAATCTGTGGAGGTCCTTTTGGAGTGTGTGGAAGTTAGAAACTTACCAGAAGGGCACAAAGGAAGTCTCTTTGTCACATTTAGTAAGACCCAAAATGATTATTTCTTTAACGCAAAGCGGAGACTGAGTATTTTGTCCGAGTCTGGAGAGAAACAGGTTGCTTGTTTCCAATGTGAACCTACTGGAGAGCTGGTTTTTGAACTGATATCTCATTCACCTTCCCACTTACCAATGATAAGGACCTATAAAACATTAGGTTCTACTTCACTCTCTCTTCAAGACTTTCTGGTCCCACTCTCTAAATTAGATGCAGAAAAATGGTTGGAGGTAGTGCCAAGTTCTGGAACTGTGAACTCAAAACCAATCTACCTTCGTGTTGCCGTGTCATTTACTATTCCAGCCTTGGCTCAACGCACATTCAACATGGTTCGTTCTCGACCATTGTCCAAAAGTTCTTGCTTTTTACCACTTCCTGGGAAGGTAGTAGATGCCAAGAATTTGACTCATGTGATTGATGAAACTGGTACAAAGCTTATCAGCCTGCAAATGAGGCATCCTGAAAAGGCAAAGGCAAGGGAAAACACCATTCTTGGGAAAGAGGTGATTGGAATCACCAAGTCTGGAAAAATATCTACTCTTGCAAAGTCTGTTGGGACTGGCTGGACTTTGATGGATTCTCACTGGTCCCTTCATCGTGAAAAGAATTCCAATGGAGATGGCCACCTCTTTGTTCTCCACGGAGAGAAGATGGTCAAAATATTCCACAGAAGAAAGCTGGACTATGAACCTAAACATTGCGACAAACATAAAAGTGAACAAGATTTCATGACCTTGGTTGAATTCTCTGCTGAAAATCCTTATGGCAAAGCAGTGGCATTGCTTGACCTAAAATCCGGATGTGTCCAGGTAAAGGAAGAATGGATTCTAGTTCTTGGGATCACGCTGGCTTTTATATTATCTGATATGCTGAAGAAGGAAAGATATGACATTTTTGCTGTTAATGCCAAGGAGATGGGCAGTGTAGCTGAGGAAATTAATGAGAACCATGACTCCCATGAGGGAGTCAAAACAACCAACTTGACCTCTTCAGGAGTAACCGAGGTTGAGCAGAACAATGGTGTGGCTGAAGGACATGCAATGATGCTGAAAAAAAGTGGAGGATGTGGTAGTGGCTGTGGAGATGTAACAAGGAGTGAGGCTTCTGCAGGATGTGGCAGTGATTGCGGTGGAGGATGTGGGAGCATGGTGAAGAGTGGTGGCTGTGGCAGTGGTTGTGGTGGTGGCTGTGGAAGCATGCTGAGGAGTGGGGGCTGCGGTGGATGTGGAGGTAGTGGTGGTTGTGGCGGTGGCTGTGGAAGCATGCTGAAGAGTGGGGGCTGCGGTGGCTGTGGAGGTAGTGGTGGTTGTGGCGGTGGCTGTGGAAGCATGCTGAAGAGTGGTGGCTGCGGTGGCTGTGGTGGTTGTGGTGGTGGATGTGGAAGCCCTTTCAAGAGCAGTGAAAACACTTCTGGTAACTCGTGCGCCGAGGAACACCAAAGGGGAACACCCAGTCATGTGAGTGAGGGAGCAGTTGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006970 GO:0008150 GO:0009628 GO:0009787 GO:0009966 GO:0009987 GO:0010646 GO:0010928 GO:0016020 GO:0023051 GO:0033554 GO:0044464 GO:0048580 GO:0048583 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051716 GO:0065007 GO:0071214 GO:0071470 GO:0071944 GO:0104004 GO:1901419 GO:1905957 GO:2000026 GO:2000028 GO:2000241

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GRDP-like

Protein Analysis

934

Amino Acids

102.2

Weight (kDa)

8.3

Isoelectric Point (pI)

44.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GRDP-like	PF07173	75 - 181	2.4e-09	Glycine-rich domain-containing protein-like
GRDP-like	PF07173	177 - 321	6.8e-58	Glycine-rich domain-containing protein-like
C2_GRDP	PF25334	408 - 540	2.4e-59	C2 domain of glycine-rich domain-containing protein
GRDP_C	PF25335	578 - 711	4.9e-38	Glycine Rich Domain Protein C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0002521)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G22660 AT2G22660 AT2G22660 AT4G37900 AT4G37900
fragaria_vesca	FvH4_1g18970 FvH4_1g18970 FvH4_1g18970 FvH4_5g33890 FvH4_5g33890 FvH4_5g33890
malus_domestica	MD01G1044900.v1.1 MD08G1208800.v1.1 MD08G1209100.v1.1
prunus_persica	Prupe.1G539900_v2.0.a1 Prupe.6G209600_v2.0.a1
pyrus_communis	pycom08g18020
rosa_chinensis	RchiOBHm_Chr2g0109991 RchiOBHm_Chr7g0233251 RchiOBHm_Chr7g0233261
rosa_laevigata	RLG00000001282 RLG00000017776
rosa_multiflora	Rmu_co8210630.1_g000001 Rmu_sc0004650.1_g000001 Rmu_sc0008314.1_g000009 Rmu_sc0035251.1_g000001 Rmu_sc0035252.1_g000001
rosa_roxburghii	Rroxscaffold_2G00133800
rosa_rugosa	Rorug02G0165000 Rorug07G0279600
rosa_samantha	Rh2AG216500 Rh2BG226400 Rh2CG218700 Rh2DG222100 Rh7AG435600 Rh7AG435700 Rh7BG408000 Rh7CG454700 Rh7CG454800 Rh7DG423900
rosa_wichuraiana	Rw2G016730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1511
AccB7I	CCANNNNNTGG	3 cut(s)	1065, 1565, 1697
AccI	GTMKAC	1 cut(s)	306
AccII	CGCG	1 cut(s)	150
AccIII	TCCGGA	1 cut(s)	2117
AciI	CCGC	8 cut(s)	182, 1342, 2448, 2538, 2568, 2604, 2634, 2670
AclI	AACGTT	1 cut(s)	886
AclWI	GGATC	3 cut(s)	284, 559, 2168
AcoI	YGGCCR	1 cut(s)	1942
AcsI	RAATTY	4 cut(s)	174, 1720, 1927, 2063
AcuI	CTGAAG	8 cut(s)	320, 483, 642, 2213, 2295, 2370, 2609, 2675
AfaI	GTAC	5 cut(s)	22, 44, 410, 480, 1750
AfiI	CCNNNNNNNGG	5 cut(s)	1065, 1565, 1697, 1815, 2134
AjnI	CCWGG	4 cut(s)	205, 777, 1696, 2127
AjuI	GAANNNNNNNTTGG	2 cut(s)	1062, 1094
AloI	GAACNNNNNNTCC	2 cut(s)	2258, 2290
Alw21I	GWGCWC	1 cut(s)	1126
Alw26I	GTCTC	4 cut(s)	231, 1067, 1295, 1339
AlwI	GGATC	3 cut(s)	284, 559, 2168
Aor13HI	TCCGGA	1 cut(s)	2117
AoxI	GGCC	2 cut(s)	380, 1942
ApeKI	GCWGC	9 cut(s)	187, 317, 320, 331, 347, 1181, 2535, 2601, 2667
ApoI	RAATTY	4 cut(s)	174, 1720, 1927, 2063
AseI	ATTAAT	1 cut(s)	2259
Asp700I	GAANNNNTTC	2 cut(s)	680, 2145
AspLEI	GCGC	3 cut(s)	150, 187, 2747
AspS9I	GGNCC	6 cut(s)	380, 467, 1237, 1464, 1513, 1908
AsuHPI	GGTGA	5 cut(s)	923, 1431, 1828, 1837, 2482
AvaII	GGWCC	5 cut(s)	467, 1237, 1464, 1513, 1908
BaeGI	GKGCMC	1 cut(s)	1281
BalI	TGGCCA	1 cut(s)	1944
BauI	CACGAG	1 cut(s)	2740
Bbv12I	GWGCWC	1 cut(s)	1126
BbvCI	CCTCAGC	2 cut(s)	2250, 2522
BbvI	GCAGC	9 cut(s)	199, 304, 332, 334, 343, 1168, 2522, 2588, 2654
BccI	CCATC	7 cut(s)	165, 244, 1196, 1888, 1934, 1966, 2230
BceAI	ACGGC	1 cut(s)	1589
BciT130I	CCWGG	4 cut(s)	207, 779, 1698, 2129
BciVI	GTATCC	2 cut(s)	654, 793
BcoDI	GTCTC	4 cut(s)	231, 1067, 1295, 1339
BfaI	CTAG	3 cut(s)	831, 1158, 2150
BfmI	CTRYAG	3 cut(s)	318, 403, 2427
BfuI	GTATCC	2 cut(s)	654, 793
BglII	AGATCT	1 cut(s)	704
Bme1390I	CCNGG	4 cut(s)	207, 779, 1698, 2129
Bme18I	GGWCC	5 cut(s)	467, 1237, 1464, 1513, 1908
BmgT120I	GGNCC	6 cut(s)	380, 467, 1237, 1464, 1513, 1908
BmiI	GGNNCC	3 cut(s)	425, 1515, 1910
BmrFI	CCNGG	4 cut(s)	207, 779, 1698, 2129
BmrI	ACTGGG	1 cut(s)	2766
BmsI	GCATC	7 cut(s)	420, 949, 1193, 1522, 1702, 1786, 2355
BmuI	ACTGGG	1 cut(s)	2766
BoxI	GACNNNNGTC	1 cut(s)	1666
BpmI	CTGGAG	2 cut(s)	1389, 1428
Bpu10I	CCTNAGC	2 cut(s)	2250, 2522
Bsa29I	ATCGAT	2 cut(s)	86, 364
BsaBI	GATNNNNATC	3 cut(s)	88, 360, 1832
BsaI	GGTCTC	1 cut(s)	1067
BsaJI	CCNNGG	7 cut(s)	1626, 1697, 1960, 2055, 2229, 2322, 2748
BsaWI	WCCGGW	1 cut(s)	2117
BsaXI	ACNNNNNCTCC	6 cut(s)	1336, 1366, 1401, 1431, 2258, 2288
Bsc4I	CCNNNNNNNGG	5 cut(s)	1065, 1565, 1697, 1815, 2134
Bse1I	ACTGG	6 cut(s)	524, 1411, 1750, 1885, 1910, 2772
Bse3DI	GCAATG	5 cut(s)	1070, 1113, 2017, 2099, 2367
Bse8I	GATNNNNATC	3 cut(s)	88, 360, 1832
BseAI	TCCGGA	1 cut(s)	2117
BseBI	CCWGG	4 cut(s)	207, 779, 1698, 2129
BseCI	ATCGAT	2 cut(s)	86, 364
BseDI	CCNNGG	7 cut(s)	1626, 1697, 1960, 2055, 2229, 2322, 2748
BseJI	GATNNNNATC	3 cut(s)	88, 360, 1832
BseLI	CCNNNNNNNGG	5 cut(s)	1065, 1565, 1697, 1815, 2134
BseMI	GCAATG	5 cut(s)	1070, 1113, 2017, 2099, 2367
BseMII	CTCAG	6 cut(s)	69, 299, 699, 1340, 2241, 2513
BseNI	ACTGG	6 cut(s)	524, 1411, 1750, 1885, 1910, 2772
BseRI	GAGGAG	1 cut(s)	2539
BseSI	GKGCMC	1 cut(s)	1281
BseXI	GCAGC	9 cut(s)	199, 304, 332, 334, 343, 1168, 2522, 2588, 2654
Bsh1236I	CGCG	1 cut(s)	150
Bsh1285I	CGRYCG	1 cut(s)	86
BshFI	GGCC	2 cut(s)	382, 1944
BshVI	ATCGAT	2 cut(s)	86, 364
BsiEI	CGRYCG	1 cut(s)	86
BsiHKAI	GWGCWC	1 cut(s)	1126
BsiSI	CCGG	2 cut(s)	199, 2118
BslFI	GGGAC	4 cut(s)	436, 1499, 1891, 1894
BslI	CCNNNNNNNGG	5 cut(s)	1065, 1565, 1697, 1815, 2134
BsmAI	GTCTC	4 cut(s)	231, 1067, 1295, 1339
BsmBI	CGTCTC	1 cut(s)	231
BsmFI	GGGAC	4 cut(s)	436, 1499, 1891, 1894
BsnI	GGCC	2 cut(s)	382, 1944
Bso31I	GGTCTC	1 cut(s)	1067
Bsp1286I	GDGCHC	2 cut(s)	1126, 1281
Bsp13I	TCCGGA	1 cut(s)	2117
Bsp143I	GATC	9 cut(s)	83, 276, 355, 537, 564, 704, 736, 805, 2160
BspACI	CCGC	8 cut(s)	182, 1342, 2448, 2538, 2568, 2604, 2634, 2670
BspANI	GGCC	2 cut(s)	382, 1944
BspCNI	CTCAG	6 cut(s)	70, 298, 698, 1341, 2242, 2514
BspDI	ATCGAT	2 cut(s)	86, 364
BspEI	TCCGGA	1 cut(s)	2117
BspFNI	CGCG	1 cut(s)	150
BspHI	TCATGA	1 cut(s)	2049
BspLI	GGNNCC	3 cut(s)	425, 1515, 1910
BspMAI	CTGCAG	2 cut(s)	322, 2431
BspPI	GGATC	3 cut(s)	284, 559, 2168
BspTNI	GGTCTC	1 cut(s)	1067
BsrDI	GCAATG	5 cut(s)	1070, 1113, 2017, 2099, 2367
BsrI	ACTGG	6 cut(s)	524, 1411, 1750, 1885, 1910, 2772
BssECI	CCNNGG	7 cut(s)	1626, 1697, 1960, 2055, 2229, 2322, 2748
BssMI	GATC	9 cut(s)	83, 276, 355, 537, 564, 704, 736, 805, 2160
BssSI	CACGAG	1 cut(s)	2740
BssT1I	CCWWGG	3 cut(s)	1626, 2055, 2229
Bst2BI	CACGAG	1 cut(s)	2740
Bst2UI	CCWGG	4 cut(s)	207, 779, 1698, 2129
Bst4CI	ACNGT	2 cut(s)	545, 1573
Bst6I	CTCTTC	6 cut(s)	1065, 1503, 2314, 2468, 2585, 2651
BstAPI	GCANNNNNTGC	1 cut(s)	2445
BstC8I	GCNNGC	8 cut(s)	192, 384, 435, 1766, 2169, 2519, 2585, 2651
BstDEI	CTNAG	7 cut(s)	78, 285, 664, 685, 1349, 2250, 2522
BstDSI	CCRYGG	1 cut(s)	1960
BstFNI	CGCG	1 cut(s)	150
BstHHI	GCGC	3 cut(s)	150, 187, 2747
BstKTI	GATC	9 cut(s)	86, 279, 358, 540, 567, 707, 739, 808, 2163
BstMAI	GTCTC	4 cut(s)	231, 1067, 1295, 1339
BstMBI	GATC	9 cut(s)	83, 276, 355, 537, 564, 704, 736, 805, 2160
BstMCI	CGRYCG	1 cut(s)	86
BstNI	CCWGG	4 cut(s)	207, 779, 1698, 2129
BstNSI	RCATGY	4 cut(s)	2360, 2521, 2587, 2653
BstPAI	GACNNNNGTC	1 cut(s)	1666
BstSCI	CCNGG	4 cut(s)	205, 777, 1696, 2127
BstSFI	CTRYAG	3 cut(s)	318, 403, 2427
BstSLI	GKGCMC	1 cut(s)	1281
BstUI	CGCG	1 cut(s)	150
BstV1I	GCAGC	9 cut(s)	199, 304, 332, 334, 343, 1168, 2522, 2588, 2654
BstX2I	RGATCY	2 cut(s)	564, 704
BstXI	CCANNNNNNTGG	1 cut(s)	2236
BstYI	RGATCY	2 cut(s)	564, 704
Bsu15I	ATCGAT	2 cut(s)	86, 364
BsuI	GTATCC	2 cut(s)	654, 793
BsuRI	GGCC	2 cut(s)	382, 1944
BsuTUI	ATCGAT	2 cut(s)	86, 364
BtgI	CCRYGG	1 cut(s)	1960
BtsI	GCAGTG	5 cut(s)	2100, 2248, 2446, 2497, 2725
BtsIMutI	CAGTG	6 cut(s)	1903, 2100, 2248, 2446, 2497, 2725
Cac8I	GCNNGC	8 cut(s)	192, 384, 435, 1766, 2169, 2519, 2585, 2651
CciI	TCATGA	1 cut(s)	2049
CfoI	GCGC	3 cut(s)	150, 187, 2747
Cfr13I	GGNCC	6 cut(s)	380, 467, 1237, 1464, 1513, 1908
ClaI	ATCGAT	2 cut(s)	86, 364
CsiI	ACCWGGT	2 cut(s)	205, 777
Csp6I	GTAC	5 cut(s)	21, 43, 409, 479, 1749
CviQI	GTAC	5 cut(s)	21, 43, 409, 479, 1749
DdeI	CTNAG	7 cut(s)	78, 285, 664, 685, 1349, 2250, 2522
DpnI	GATC	9 cut(s)	85, 278, 357, 539, 566, 706, 738, 807, 2162
DpnII	GATC	9 cut(s)	83, 276, 355, 537, 564, 704, 736, 805, 2160
DrdI	GACNNNNNNGTC	1 cut(s)	1511
DseDI	GACNNNNNNGTC	1 cut(s)	1511
EaeI	YGGCCR	1 cut(s)	1942
Eam1104I	CTCTTC	6 cut(s)	1065, 1503, 2314, 2468, 2585, 2651
EarI	CTCTTC	6 cut(s)	1065, 1503, 2314, 2468, 2585, 2651
Eco130I	CCWWGG	3 cut(s)	1626, 2055, 2229
Eco31I	GGTCTC	1 cut(s)	1067
Eco32I	GATATC	3 cut(s)	90, 368, 1431
Eco47I	GGWCC	5 cut(s)	467, 1237, 1464, 1513, 1908
Eco57I	CTGAAG	8 cut(s)	320, 483, 642, 2213, 2295, 2370, 2609, 2675
EcoO109I	RGGNCCY	4 cut(s)	380, 467, 1237, 1464
EcoRI	GAATTC	2 cut(s)	1927, 2063
EcoRII	CCWGG	4 cut(s)	205, 777, 1696, 2127
EcoRV	GATATC	3 cut(s)	90, 368, 1431
EcoT14I	CCWWGG	3 cut(s)	1626, 2055, 2229
EcoT22I	ATGCAT	1 cut(s)	1113
ErhI	CCWWGG	3 cut(s)	1626, 2055, 2229
Esp3I	CGTCTC	1 cut(s)	231
FalI	AAGNNNNNCTT	2 cut(s)	831, 863
FaqI	GGGAC	4 cut(s)	436, 1499, 1891, 1894
FblI	GTMKAC	1 cut(s)	306
FspBI	CTAG	3 cut(s)	831, 1158, 2150
GlaI	GCGC	3 cut(s)	149, 186, 2746
GsuI	CTGGAG	2 cut(s)	1389, 1428
HaeIII	GGCC	2 cut(s)	382, 1944
HapII	CCGG	2 cut(s)	199, 2118
HhaI	GCGC	3 cut(s)	150, 187, 2747
Hin6I	GCGC	3 cut(s)	148, 185, 2745
HinP1I	GCGC	3 cut(s)	148, 185, 2745
HincII	GTYRAC	2 cut(s)	154, 913
HindII	GTYRAC	2 cut(s)	154, 913
HindIII	AAGCTT	2 cut(s)	124, 1754
HpaI	GTTAAC	1 cut(s)	154
HpaII	CCGG	2 cut(s)	199, 2118
HphI	GGTGA	5 cut(s)	923, 1431, 1828, 1837, 2482
Hpy166II	GTNNAC	6 cut(s)	154, 307, 913, 1400, 1576, 2039
Hpy8I	GTNNAC	6 cut(s)	154, 307, 913, 1400, 1576, 2039
HpyCH4III	ACNGT	2 cut(s)	545, 1573
HpyCH4IV	ACGT	3 cut(s)	38, 240, 886
HpyCH4V	TGCA	9 cut(s)	320, 940, 1111, 1184, 1535, 1768, 1865, 2360, 2429
HpyF3I	CTNAG	7 cut(s)	78, 285, 664, 685, 1349, 2250, 2522
HpySE526I	ACGT	3 cut(s)	38, 240, 886
HspAI	GCGC	3 cut(s)	148, 185, 2745
Kpn2I	TCCGGA	1 cut(s)	2117
KspAI	GTTAAC	1 cut(s)	154
Kzo9I	GATC	9 cut(s)	83, 276, 355, 537, 564, 704, 736, 805, 2160
LmnI	GCTCC	7 cut(s)	67, 429, 991, 1103, 1129, 2463, 2789
Lsp1109I	GCAGC	9 cut(s)	199, 304, 332, 334, 343, 1168, 2522, 2588, 2654
LweI	GCATC	7 cut(s)	420, 949, 1193, 1522, 1702, 1786, 2355
MabI	ACCWGGT	2 cut(s)	205, 777
MaeI	CTAG	3 cut(s)	831, 1158, 2150
MaeII	ACGT	3 cut(s)	38, 240, 886
MaeIII	GTNAC	5 cut(s)	509, 1297, 2317, 2407, 2735
MalI	GATC	9 cut(s)	85, 278, 357, 539, 566, 706, 738, 807, 2162
MboI	GATC	9 cut(s)	83, 276, 355, 537, 564, 704, 736, 805, 2160
MfeI	CAATTG	1 cut(s)	935
MflI	RGATCY	2 cut(s)	564, 704
MhlI	GDGCHC	2 cut(s)	1126, 1281
MlsI	TGGCCA	1 cut(s)	1944
MluCI	AATT	9 cut(s)	174, 574, 935, 1206, 1526, 1720, 1927, 2063, 2256
MluNI	TGGCCA	1 cut(s)	1944
MlyI	GAGTC	8 cut(s)	458, 596, 772, 1001, 1373, 1720, 2267, 2295
MmeI	TCCRAC	2 cut(s)	923, 1527
Mox20I	TGGCCA	1 cut(s)	1944
Mph1103I	ATGCAT	1 cut(s)	1113
MroI	TCCGGA	1 cut(s)	2117
MroXI	GAANNNNTTC	2 cut(s)	680, 2145
MscI	TGGCCA	1 cut(s)	1944
MseI	TTAA	9 cut(s)	8, 153, 671, 753, 1224, 1332, 2223, 2259, 2800
MslI	CAYNNNNRTG	2 cut(s)	2034, 2781
Msp20I	TGGCCA	1 cut(s)	1944
MspA1I	CMGCKG	1 cut(s)	749
MspI	CCGG	2 cut(s)	199, 2118
MspR9I	CCNGG	4 cut(s)	207, 779, 1698, 2129
MunI	CAATTG	1 cut(s)	935
MvaI	CCWGG	4 cut(s)	207, 779, 1698, 2129
MvnI	CGCG	1 cut(s)	150
NdeII	GATC	9 cut(s)	83, 276, 355, 537, 564, 704, 736, 805, 2160
NlaIV	GGNNCC	3 cut(s)	425, 1515, 1910
NmeAIII	GCCGAG	1 cut(s)	2773
NmuCI	GTSAC	2 cut(s)	509, 1297
NsiI	ATGCAT	1 cut(s)	1113
NspI	RCATGY	4 cut(s)	2360, 2521, 2587, 2653
PaeI	GCATGC	3 cut(s)	2521, 2587, 2653
PagI	TCATGA	1 cut(s)	2049
PdmI	GAANNNNTTC	2 cut(s)	680, 2145
PfeI	GAWTC	4 cut(s)	361, 1833, 1898, 2146
PflFI	GACNNNGTC	2 cut(s)	207, 762
PflMI	CCANNNNNTGG	3 cut(s)	1065, 1565, 1697
Ple19I	CGATCG	1 cut(s)	86
PleI	GAGTC	8 cut(s)	457, 596, 771, 1001, 1372, 1720, 2267, 2294
PpsI	GAGTC	8 cut(s)	457, 596, 771, 1001, 1372, 1720, 2267, 2294
PpuMI	RGGWCCY	3 cut(s)	467, 1237, 1464
PshAI	GACNNNNGTC	1 cut(s)	1666
PshBI	ATTAAT	1 cut(s)	2259
Psp1406I	AACGTT	1 cut(s)	886
Psp5II	RGGWCCY	3 cut(s)	467, 1237, 1464
Psp6I	CCWGG	4 cut(s)	205, 777, 1696, 2127
PspGI	CCWGG	4 cut(s)	205, 777, 1696, 2127
PspN4I	GGNNCC	3 cut(s)	425, 1515, 1910
PspPI	GGNCC	6 cut(s)	380, 467, 1237, 1464, 1513, 1908
PspPPI	RGGWCCY	3 cut(s)	467, 1237, 1464
PstI	CTGCAG	2 cut(s)	322, 2431
PsuI	RGATCY	2 cut(s)	564, 704
PsyI	GACNNNGTC	2 cut(s)	207, 762
PvuI	CGATCG	1 cut(s)	86
PvuII	CAGCTG	1 cut(s)	749
RsaI	GTAC	5 cut(s)	22, 44, 410, 480, 1750
RsaNI	GTAC	5 cut(s)	21, 43, 409, 479, 1749
RseI	CAYNNNNRTG	2 cut(s)	2034, 2781
SaqAI	TTAA	9 cut(s)	8, 153, 671, 753, 1224, 1332, 2223, 2259, 2800
Sau3AI	GATC	9 cut(s)	83, 276, 355, 537, 564, 704, 736, 805, 2160
Sau96I	GGNCC	6 cut(s)	380, 467, 1237, 1464, 1513, 1908
SchI	GAGTC	8 cut(s)	458, 596, 772, 1001, 1373, 1720, 2267, 2295
ScrFI	CCNGG	4 cut(s)	207, 779, 1698, 2129
SduI	GDGCHC	2 cut(s)	1126, 1281
SexAI	ACCWGGT	2 cut(s)	205, 777
SfaNI	GCATC	7 cut(s)	420, 949, 1193, 1522, 1702, 1786, 2355
SfcI	CTRYAG	3 cut(s)	318, 403, 2427
SinI	GGWCC	5 cut(s)	467, 1237, 1464, 1513, 1908
SmiMI	CAYNNNNRTG	2 cut(s)	2034, 2781
SphI	GCATGC	3 cut(s)	2521, 2587, 2653
Sse9I	AATT	9 cut(s)	174, 574, 935, 1206, 1526, 1720, 1927, 2063, 2256
SsiI	CCGC	8 cut(s)	182, 1342, 2448, 2538, 2568, 2604, 2634, 2670
SspI	AATATT	1 cut(s)	1983
SspMI	CTAG	3 cut(s)	831, 1158, 2150
StyD4I	CCNGG	4 cut(s)	205, 777, 1696, 2127
StyI	CCWWGG	3 cut(s)	1626, 2055, 2229
TaaI	ACNGT	2 cut(s)	545, 1573
TaiI	ACGT	3 cut(s)	41, 243, 889
TaqI	TCGA	8 cut(s)	86, 235, 364, 540, 712, 816, 1191, 1660
TasI	AATT	9 cut(s)	174, 574, 935, 1206, 1526, 1720, 1927, 2063, 2256
TatI	WGTACW	1 cut(s)	42
TauI	GCSGC	1 cut(s)	185
TfiI	GAWTC	4 cut(s)	361, 1833, 1898, 2146
Tru1I	TTAA	9 cut(s)	8, 153, 671, 753, 1224, 1332, 2223, 2259, 2800
Tru9I	TTAA	9 cut(s)	8, 153, 671, 753, 1224, 1332, 2223, 2259, 2800
TscAI	CASTG	6 cut(s)	1910, 2100, 2248, 2446, 2497, 2725
TseFI	GTSAC	2 cut(s)	509, 1297
TseI	GCWGC	9 cut(s)	187, 317, 320, 331, 347, 1181, 2535, 2601, 2667
Tsp45I	GTSAC	2 cut(s)	509, 1297
TspDTI	ATGAA	8 cut(s)	390, 812, 839, 984, 1755, 1904, 2022, 2038
TspGWI	ACGGA	2 cut(s)	150, 1977
TspRI	CASTG	6 cut(s)	1910, 2100, 2248, 2446, 2497, 2725
Tth111I	GACNNNGTC	2 cut(s)	207, 762
Van91I	CCANNNNNTGG	3 cut(s)	1065, 1565, 1697
VpaK11BI	GGWCC	5 cut(s)	467, 1237, 1464, 1513, 1908
VspI	ATTAAT	1 cut(s)	2259
XapI	RAATTY	4 cut(s)	174, 1720, 1927, 2063
XceI	RCATGY	4 cut(s)	2360, 2521, 2587, 2653
XcmI	CCANNNNNNNNNTGG	1 cut(s)	736
XmiI	GTMKAC	1 cut(s)	306
XmnI	GAANNNNTTC	2 cut(s)	680, 2145
XspI	CTAG	3 cut(s)	831, 1158, 2150
Zsp2I	ATGCAT	1 cut(s)	1113

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.