RLG00000017776

Glycine-rich domain-containing protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0002521
Physical Location & Seq
Reverse (-)
21072365 .. 21079151
6787 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017776

Sequence Viewer

Length: 3255 bp
ATGGCTGATGAGGATAGAAACCTAGTGGAAATTTTGGAGGAGCATGCCGAGATTGACATTGCGAAATATATCAATTATGTAAGTGCCCCAGAGGCAGGTGCTACTGCAACGTTTTCTGGTACCACGCGTGACACCTTTGATGGAAAAGCAGTACTGGAGTTGAGATACGAAGCATATGTTCCCATGGCGATACGATGCATGAAATCCATTTGTTCATCTGCTAGATCCTCCTGGAATCTTGTCTCCATTGCAGTTGCCCACCGCCTTGGCCCAGTTCCTGTTGGTAAAACGAGTGTTTTCATTGCAGTCTCATCTGTTCATCGAGTTGATTCCTTAGATGCTTGTAAGTTTATAATTGATGAGATAAAGGCGTCAGTTCCAATATGGAAGAAGGAGGTATATACTAATGGAGAGGTTTGGAAGGAGAACTTAGAGTTCTTGGAGAGGAGGTTGGATCTCGGGAAAAAAGAAGGCATATGTAATGAGAAAGACTCTGAAGCACAAAGACATATTAGGAAGAGCTGCTGTGGTGCTAAGGTAAAGGTTGTAGAAGAAGGAGATTCTGAAACCGGGAAGAGTCGTACTTTGTACATTGTCAAGATCACACGCTATAAATATCTGACTGCAACTCGTAAGAAAGCAGAAGCTTCAATCAGTCTCGGACCCCACGCTAACCGTCGTCGGAACTATTTTCAGATAGAGATAGAGATGGAGATGGAGATGGAGAAGGAACAGGAGCTTGAGTGGACCAAAGCACAGAGCATTGGAATAAGCGTAGACCTTGTGGCTGCAGCAAAAAAGCAGCTTCAGTTTCTTGCTGCTGTTGATAGGAATCGGTTTCTGTACGAAGGGCCGGCTCTTAACAGGGCTATCTATAGGTACAACGCTTGTTGGCTTCCGTTGCTTGCCAAACATTCAGACTCCCAGGTTTCTGAGGGACCTTTGGTTGTACCTCTTGATTGTGAATGGATTTGGCATTGTCACAGGCTAAATCCGGTTCGATACAAGACTGACTGTGAAGAACTTTATGGAAAGATTCTTGACAACTCGAATGTTGTTTCCACTGTTGAAGGTTCTTGTAAAAGTAAAACTGAAGAAATTTGGAATAGTCTGTATCCAGCAGAACCCTACAACTTCAACTTACAAAAGGCATTGTCCGAGGATATATCTGAAAGGATTTCCAAACTTGACAAATGCACCAAATATGATCTGGTTTCAGCTGTTAAAAGACAATCTCCTTTCTTTTACCAGGTATCTCCACCCCATATGAACCATGATCTGTTTCTTGAAGGAGCTGCGTCTAGATATAAAGGGTTTCTTCATTTAATCAAGAGCAATAATGAGAAGTCTCTACGACGTTTCTGCGTTCCGACATATGATATTGATCTTATCTGGCATACTCACCAATTGCATCCTGTATCCTATTGTAAAGATCTGCATGAACTACTTGGAAAGGTATTGGAGCATGACGACATGGATTCGGACAGAACAAAAGGGAAGAAGCTGGATACTGGTTTCTCTGTAACCACCAAGCAATGGGAAGAGGCATTTGGTACCAGGTATTGGAGAGCAGGGGCAATGTATAGAGGCAGGGCTCCATCTCCTCTCACAACCACTCCTTATCAGTCTAACATCATTAGCAAGGATGTTATTGCATGTCCTGATCATCAGAAAGTAATTGAGCTTCCAGAGGTGAAAGCTGTGGAGGTCCTTTTGGAGTTTCTGGAAGTTAGGAACTTACCAGAAGGACACAAAGGGCTTCTCTTTGCCTCATTCAGTAAGACTACAAAGGATATATTTTTCAATGCTAAGCGGCGACTCAGCATTTTTTCCGAGTTTGGAGAGAAACAGGTTGCTTCTTTCCAGTGTGAACCTACTGGAGAGCTGCTTTTTGAACTCATGTCCCATTCACCTTTATATATACCAATGAAAAGGGCATATAAAACATTAGGTTCTGCTTCGTTCTCTCTTCAAGACTGTCTAGTCCCACCCTCTAAATTATATGCGGAAAAATGGTTGGAGATGGTGCCAAGCTCTAAAGTTGGGAACTTAAAACCAATCTACATGCGGTTTGCTATGTCTTTTACTATTCCAACCATAGCACAACACACACTTCATGTGGTTCGTTCTCGACCATTTTCAAAAAGTTCTTGCTTTTTCCCCTTTGCTGGGAAGAATCAAGATGCCAAGAATTGGACACAAGTGATTGATGAAACTGGAACAGAGGTCCTCCGCCTGCAAATGAGGGATACTGAAAAGGAAAAGGTAAAGGGAATCAGCGTTCCAAGGAAAGAGGTGGTTGGAATTACTAAGACTGGGAAAATATGTACTCTTGCAGAATTTGTGGGGACTGGTTGGTCTTTGATAGATTCACACTGGTCCCTTCATCGTGAGAAAAATTCCGAGGGATACCTCTTTTTGCTAAAGGGAAAAAAGATGGTCAAATTCTTCCCAGGCAGAAAGCTGGATTATGAACATAAGAATTATGAGGAACATACAACTGAAGATAAAACTGAACAACACTTCATGACCGTAGTTGAATTCTCTGCTGAAGATCCTTATGGCAAAGCAGTGGCATTGCTTGACCTAAAATCTGGGTGCATCAAGGTAAAGGAAGAATCAATAACAGTTCCTGGGGTCGTAATGGCTTTTATACTGTCGGATAAGCTAAAGAAGGAAAGATATGACGGATTTGCTGTTAATGCTGCGGAGATGGGTAGTGTCGAGGAAGAAATTAAAGCGAACCCTGAGGAAGGCAAAGGAACCAACTTGAGCTCTTCTGGAGCAAGTGCAGTTAACCTTAAATCTGAGGTGGTTGAAGGAAATGTAGTGACGTCTCAAAAAGGTGGAGGCTGTGGTGGAGCATGTGGCAGTGGGTGTGGAAATGCAACAAGGAGTGCCGGCGCTGGTGGATGTGGAAGTGGCTGTGGTGGAGGATGTGGAAACATGGAGAAGAGTAGCGGTTGCGGCAGTGGATGTGGTGGAGGATGTGGAAACTTGGTGAAGAGTGGTGGCTGTGGAGGCGGCTGTGGGGGTGGCTGTGGAAACATACTGAAGAGTGGTGGCTGTGGTGGCTGTGGCAGCGGCTGTGGCGGTGGCTGTGGGAACATACTGAAGAGTGGTGGCTGCGGTGGGTGTGGTGGTAGCGGTGGTTGTGGTGGTGGGTGCGGAGGCTTATTCAAGGGTAATGGTTATCATGAAAATACATCTGGTAACTCATGTGCCGAAGACCACCCGAAGGCAGTAGTTGTTTAA

Protein Analysis

1085

Amino Acids

119.03

Weight (kDa)

8.04

Isoelectric Point (pI)

41.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MoaE PF02391 15 - 124 6.3e-36 MoaE protein
GRDP-like PF07173 229 - 338 4.7e-10 Glycine-rich domain-containing protein-like
GRDP-like PF07173 334 - 478 1.6e-57 Glycine-rich domain-containing protein-like
C2_GRDP PF25334 565 - 697 6.7e-54 C2 domain of glycine-rich domain-containing protein
GRDP_C PF25335 735 - 870 3.9e-36 Glycine Rich Domain Protein C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 353
AarI CACCTGC 1 cut(s) 86
AasI GACNNNNNNGTC 2 cut(s) 1982, 2356
AatII GACGTC 1 cut(s) 2837
Acc36I ACCTGC 1 cut(s) 86
Acc65I GGTACC 2 cut(s) 119, 1553
AccB1I GGYRCC 3 cut(s) 119, 1553, 2026
AccB7I CCANNNNNTGG 3 cut(s) 1536, 1563, 2193
AccI GTMKAC 1 cut(s) 777
AccII CGCG 1 cut(s) 127
AclI AACGTT 1 cut(s) 110
AclWI GGATC 3 cut(s) 219, 462, 2549
AcsI RAATTY 6 cut(s) 30, 1098, 2339, 2398, 2444, 2540
AcuI CTGAAG 7 cut(s) 516, 791, 1113, 2523, 2571, 3074, 3134
AcyI GRCGYC 2 cut(s) 371, 2834
AfaI GTAC 9 cut(s) 121, 153, 583, 590, 845, 881, 951, 1555, 2329
AfiI CCNNNNNNNGG 8 cut(s) 95, 1536, 1563, 2168, 2169, 2193, 2753, 3238
AflIII ACRYGT 1 cut(s) 125
AjnI CCWGG 6 cut(s) 230, 924, 1248, 1556, 2452, 2632
AjuI GAANNNNNNNTTGG 2 cut(s) 1533, 1565
AloI GAACNNNNNNTCC 2 cut(s) 2265, 2297
Alw21I GWGCWC 1 cut(s) 2777
Alw26I GTCTC 5 cut(s) 247, 313, 662, 1353, 2841
AlwI GGATC 3 cut(s) 219, 462, 2549
AlwNI CAGNNNCTG 3 cut(s) 278, 2633, 3087
Ama87I CYCGRG 1 cut(s) 458
AoxI GGCC 2 cut(s) 268, 851
ApoI RAATTY 6 cut(s) 30, 1098, 2339, 2398, 2444, 2540
Asp718I GGTACC 2 cut(s) 119, 1553
AspLEI GCGC 1 cut(s) 2906
AspS9I GGNCC 8 cut(s) 269, 662, 747, 851, 938, 1708, 2227, 2379
AsuC2I CCSGG 1 cut(s) 571
AsuHPI GGTGA 4 cut(s) 1394, 1705, 1902, 3013
AvaI CYCGRG 1 cut(s) 458
AvaII GGWCC 6 cut(s) 662, 747, 938, 1708, 2227, 2379
AxyI CCTNAGG 1 cut(s) 2748
BaeGI GKGCMC 1 cut(s) 88
BanI GGYRCC 3 cut(s) 119, 1553, 2026
BanII GRGCYC 2 cut(s) 1597, 2777
BarI GAAGNNNNNNTAC 2 cut(s) 1668, 1700
BbsI GAAGAC 1 cut(s) 3234
Bbv12I GWGCWC 1 cut(s) 2777
BccI CCATC 8 cut(s) 134, 703, 709, 715, 1606, 2017, 2431, 2707
BcgI CGANNNNNNTGC 2 cut(s) 1344, 1378
BciT130I CCWGG 6 cut(s) 232, 926, 1250, 1558, 2454, 2634
BciVI GTATCC 5 cut(s) 1125, 1429, 1501, 2242, 2402
BclI TGATCA 1 cut(s) 1663
BcnI CCSGG 1 cut(s) 571
BcoDI GTCTC 5 cut(s) 247, 313, 662, 1353, 2841
BfaI CTAG 4 cut(s) 23, 222, 1302, 1982
BfmI CTRYAG 2 cut(s) 789, 874
BfoI RGCGCY 1 cut(s) 2907
BfuAI ACCTGC 1 cut(s) 86
BfuI GTATCC 5 cut(s) 1125, 1429, 1501, 2242, 2402
BglI GCCNNNNNGGC 1 cut(s) 92
BglII AGATCT 1 cut(s) 1432
BlpI GCTNAGC 1 cut(s) 1809
BmcAI AGTACT 1 cut(s) 153
Bme1390I CCNGG 7 cut(s) 232, 571, 926, 1250, 1558, 2454, 2634
Bme18I GGWCC 6 cut(s) 662, 747, 938, 1708, 2227, 2379
BmeT110I CYCGRG 1 cut(s) 458
BmgT120I GGNCC 8 cut(s) 269, 662, 747, 851, 938, 1708, 2227, 2379
BmiI GGNNCC 8 cut(s) 121, 664, 939, 1555, 1596, 2028, 2381, 2764
BmrFI CCNGG 7 cut(s) 232, 571, 926, 1250, 1558, 2454, 2634
BmrI ACTGGG 2 cut(s) 266, 2325
BmsI GCATC 5 cut(s) 185, 328, 1420, 2173, 2610
BmuI ACTGGG 2 cut(s) 266, 2325
BpiI GAAGAC 1 cut(s) 3234
BplI GAGNNNNNCTC 2 cut(s) 476, 508
BpmI CTGGAG 3 cut(s) 176, 1899, 2802
Bpu10I CCTNAGC 1 cut(s) 534
Bpu1102I GCTNAGC 1 cut(s) 1809
BpuEI CTTGAG 2 cut(s) 761, 2791
BpuMI CCSGG 1 cut(s) 571
BsaBI GATNNNNATC 2 cut(s) 831, 1383
BsaHI GRCGYC 2 cut(s) 371, 2834
BsaJI CCNNGG 8 cut(s) 183, 265, 924, 1158, 2285, 2403, 2452, 2633
BsaWI WCCGGW 1 cut(s) 994
BsaXI ACNNNNNCTCC 6 cut(s) 434, 464, 728, 758, 1600, 1630
Bsc4I CCNNNNNNNGG 8 cut(s) 95, 1536, 1563, 2168, 2169, 2193, 2753, 3238
Bse118I RCCGGY 2 cut(s) 853, 2900
Bse1I ACTGG 9 cut(s) 159, 272, 1516, 1864, 1882, 2221, 2320, 2356, 2381
Bse21I CCTNAGG 1 cut(s) 2748
Bse3DI GCAATG 6 cut(s) 57, 246, 300, 1541, 1584, 2576
Bse8I GATNNNNATC 2 cut(s) 831, 1383
BseBI CCWGG 6 cut(s) 232, 926, 1250, 1558, 2454, 2634
BseDI CCNNGG 8 cut(s) 183, 265, 924, 1158, 2285, 2403, 2452, 2633
BseGI GGATG 6 cut(s) 1411, 1651, 2918, 2942, 2981, 2993
BseJI GATNNNNATC 2 cut(s) 831, 1383
BseLI CCNNNNNNNGG 8 cut(s) 95, 1536, 1563, 2168, 2169, 2193, 2753, 3238
BseMI GCAATG 6 cut(s) 57, 246, 300, 1541, 1584, 2576
BseMII CTCAG 4 cut(s) 924, 1834, 2739, 2799
BseNI ACTGG 9 cut(s) 159, 272, 1516, 1864, 1882, 2221, 2320, 2356, 2381
BseRI GAGGAG 3 cut(s) 53, 460, 1593
BseSI GKGCMC 1 cut(s) 88
BseYI CCCAGC 1 cut(s) 2168
BsgI GTGCAG 1 cut(s) 2811
Bsh1236I CGCG 1 cut(s) 127
BshFI GGCC 2 cut(s) 270, 853
BshNI GGYRCC 3 cut(s) 119, 1553, 2026
BsiHKAI GWGCWC 1 cut(s) 2777
BsiHKCI CYCGRG 1 cut(s) 458
BsiSI CCGG 4 cut(s) 570, 854, 995, 2901
BslFI GGGAC 5 cut(s) 951, 1888, 1970, 2362, 2365
BslI CCNNNNNNNGG 8 cut(s) 95, 1536, 1563, 2168, 2169, 2193, 2753, 3238
BsmAI GTCTC 5 cut(s) 247, 313, 662, 1353, 2841
BsmBI CGTCTC 1 cut(s) 2841
BsmFI GGGAC 5 cut(s) 951, 1888, 1970, 2362, 2365
BsnI GGCC 2 cut(s) 270, 853
BsoBI CYCGRG 1 cut(s) 458
Bsp1286I GDGCHC 3 cut(s) 88, 1597, 2777
Bsp1407I TGTACA 1 cut(s) 588
Bsp143I GATC 9 cut(s) 224, 454, 600, 1207, 1276, 1384, 1432, 1663, 2554
Bsp1720I GCTNAGC 1 cut(s) 1809
Bsp19I CCATGG 1 cut(s) 183
BspANI GGCC 2 cut(s) 270, 853
BspCNI CTCAG 4 cut(s) 925, 1833, 2740, 2800
BspFNI CGCG 1 cut(s) 127
BspHI TCATGA 2 cut(s) 2526, 3196
BspLI GGNNCC 8 cut(s) 121, 664, 939, 1555, 1596, 2028, 2381, 2764
BspMAI CTGCAG 1 cut(s) 793
BspMI ACCTGC 1 cut(s) 86
BspPI GGATC 3 cut(s) 219, 462, 2549
BspQI GCTCTTC 2 cut(s) 512, 2782
BspT107I GGYRCC 3 cut(s) 119, 1553, 2026
BsrDI GCAATG 6 cut(s) 57, 246, 300, 1541, 1584, 2576
BsrFI RCCGGY 2 cut(s) 853, 2900
BsrGI TGTACA 1 cut(s) 588
BsrI ACTGG 9 cut(s) 159, 272, 1516, 1864, 1882, 2221, 2320, 2356, 2381
BssAI RCCGGY 2 cut(s) 853, 2900
BssECI CCNNGG 8 cut(s) 183, 265, 924, 1158, 2285, 2403, 2452, 2633
BssMI GATC 9 cut(s) 224, 454, 600, 1207, 1276, 1384, 1432, 1663, 2554
BssNI GRCGYC 2 cut(s) 371, 2834
BssT1I CCWWGG 3 cut(s) 183, 265, 2285
Bst2UI CCWGG 6 cut(s) 232, 926, 1250, 1558, 2454, 2634
Bst4CI ACNGT 7 cut(s) 677, 1016, 1066, 1979, 2533, 2629, 2658
Bst6I CTCTTC 9 cut(s) 512, 569, 1536, 1974, 2782, 2948, 2999, 3050, 3110
BstACI GRCGYC 2 cut(s) 371, 2834
BstAUI TGTACA 1 cut(s) 588
BstC8I GCNNGC 5 cut(s) 45, 855, 906, 2237, 2902
BstDEI CTNAG 9 cut(s) 334, 430, 534, 933, 1809, 1820, 2310, 2748, 2808
BstDSI CCRYGG 1 cut(s) 183
BstENI CCTNNNNNAGG 1 cut(s) 2751
BstF5I GGATG 6 cut(s) 1411, 1651, 2918, 2942, 2981, 2993
BstFNI CGCG 1 cut(s) 127
BstH2I RGCGCY 1 cut(s) 2907
BstHHI GCGC 1 cut(s) 2906
BstKTI GATC 9 cut(s) 227, 457, 603, 1210, 1279, 1387, 1435, 1666, 2557
BstMAI GTCTC 5 cut(s) 247, 313, 662, 1353, 2841
BstMBI GATC 9 cut(s) 224, 454, 600, 1207, 1276, 1384, 1432, 1663, 2554
BstMWI GCNNNNNNNGC 8 cut(s) 92, 901, 2702, 2967, 3021, 3072, 3081, 3090
BstNI CCWGG 6 cut(s) 232, 926, 1250, 1558, 2454, 2634
BstNSI RCATGY 4 cut(s) 47, 1659, 2068, 2868
BstSCI CCNGG 7 cut(s) 230, 569, 924, 1248, 1556, 2452, 2632
BstSFI CTRYAG 2 cut(s) 789, 874
BstSLI GKGCMC 1 cut(s) 88
BstUI CGCG 1 cut(s) 127
BstV2I GAAGAC 1 cut(s) 3234
BstX2I RGATCY 4 cut(s) 224, 454, 1432, 2554
BstXI CCANNNNNNTGG 1 cut(s) 266
BstYI RGATCY 4 cut(s) 224, 454, 1432, 2554
Bsu36I CCTNAGG 1 cut(s) 2748
BsuI GTATCC 5 cut(s) 1125, 1429, 1501, 2242, 2402
BsuRI GGCC 2 cut(s) 270, 853
BtgI CCRYGG 1 cut(s) 183
BtsCI GGATG 6 cut(s) 1411, 1651, 2918, 2942, 2981, 2993
BtsI GCAGTG 3 cut(s) 2577, 2878, 2977
BtsIMutI CAGTG 6 cut(s) 1062, 1871, 2374, 2577, 2878, 2977
BveI ACCTGC 1 cut(s) 86
Cac8I GCNNGC 5 cut(s) 45, 855, 906, 2237, 2902
CaiI CAGNNNCTG 3 cut(s) 278, 2633, 3087
CciI TCATGA 2 cut(s) 2526, 3196
CfoI GCGC 1 cut(s) 2906
Cfr10I RCCGGY 2 cut(s) 853, 2900
Cfr13I GGNCC 8 cut(s) 269, 662, 747, 851, 938, 1708, 2227, 2379
CseI GACGC 2 cut(s) 360, 1287
CsiI ACCWGGT 2 cut(s) 1248, 1556
Csp6I GTAC 9 cut(s) 120, 152, 582, 589, 844, 880, 950, 1554, 2328
CviQI GTAC 9 cut(s) 120, 152, 582, 589, 844, 880, 950, 1554, 2328
DdeI CTNAG 9 cut(s) 334, 430, 534, 933, 1809, 1820, 2310, 2748, 2808
DpnI GATC 9 cut(s) 226, 456, 602, 1209, 1278, 1386, 1434, 1665, 2556
DpnII GATC 9 cut(s) 224, 454, 600, 1207, 1276, 1384, 1432, 1663, 2554
DrdI GACNNNNNNGTC 2 cut(s) 1982, 2356
DseDI GACNNNNNNGTC 2 cut(s) 1982, 2356
Eam1104I CTCTTC 9 cut(s) 512, 569, 1536, 1974, 2782, 2948, 2999, 3050, 3110
EarI CTCTTC 9 cut(s) 512, 569, 1536, 1974, 2782, 2948, 2999, 3050, 3110
EciI GGCGGA 1 cut(s) 2222
Ecl136II GAGCTC 1 cut(s) 2775
Eco130I CCWWGG 3 cut(s) 183, 265, 2285
Eco24I GRGCYC 2 cut(s) 1597, 2777
Eco47I GGWCC 6 cut(s) 662, 747, 938, 1708, 2227, 2379
Eco53kI GAGCTC 1 cut(s) 2775
Eco57I CTGAAG 7 cut(s) 516, 791, 1113, 2523, 2571, 3074, 3134
Eco81I CCTNAGG 1 cut(s) 2748
Eco88I CYCGRG 1 cut(s) 458
EcoICRI GAGCTC 1 cut(s) 2775
EcoNI CCTNNNNNAGG 1 cut(s) 2751
EcoO109I RGGNCCY 3 cut(s) 938, 1708, 2227
EcoRI GAATTC 1 cut(s) 2540
EcoRII CCWGG 6 cut(s) 230, 924, 1248, 1556, 2452, 2632
EcoT14I CCWWGG 3 cut(s) 183, 265, 2285
EcoT22I ATGCAT 1 cut(s) 200
EcoT38I GRGCYC 2 cut(s) 1597, 2777
ErhI CCWWGG 3 cut(s) 183, 265, 2285
Esp3I CGTCTC 1 cut(s) 2841
FalI AAGNNNNNCTT 4 cut(s) 413, 445, 1302, 1334
FaqI GGGAC 5 cut(s) 951, 1888, 1970, 2362, 2365
FauNDI CATATG 4 cut(s) 175, 476, 1266, 1375
FbaI TGATCA 1 cut(s) 1663
FblI GTMKAC 1 cut(s) 777
FokI GGATG 6 cut(s) 1398, 1658, 2925, 2949, 2988, 3000
FriOI GRGCYC 2 cut(s) 1597, 2777
FspBI CTAG 4 cut(s) 23, 222, 1302, 1982
GlaI GCGC 1 cut(s) 2905
GsaI CCCAGC 1 cut(s) 2172
GsuI CTGGAG 3 cut(s) 176, 1899, 2802
HaeII RGCGCY 1 cut(s) 2907
HaeIII GGCC 2 cut(s) 270, 853
HapII CCGG 4 cut(s) 570, 854, 995, 2901
HgaI GACGC 2 cut(s) 360, 1287
HhaI GCGC 1 cut(s) 2906
Hin1I GRCGYC 2 cut(s) 371, 2834
Hin6I GCGC 1 cut(s) 2904
HinP1I GCGC 1 cut(s) 2904
HincII GTYRAC 1 cut(s) 2797
HindII GTYRAC 1 cut(s) 2797
HindIII AAGCTT 1 cut(s) 645
HpaI GTTAAC 1 cut(s) 2797
HpaII CCGG 4 cut(s) 570, 854, 995, 2901
HphI GGTGA 4 cut(s) 1394, 1705, 1902, 3013
Hpy166II GTNNAC 4 cut(s) 747, 778, 1871, 2797
Hpy8I GTNNAC 4 cut(s) 747, 778, 1871, 2797
Hpy99I CGWCG 3 cut(s) 681, 684, 1359
HpyCH4III ACNGT 7 cut(s) 677, 1016, 1066, 1979, 2533, 2629, 2658
HpyCH4IV ACGT 3 cut(s) 110, 1357, 2834
HpyF10VI GCNNNNNNNGC 8 cut(s) 92, 901, 2702, 2967, 3021, 3072, 3081, 3090
HpyF3I CTNAG 9 cut(s) 334, 430, 534, 933, 1809, 1820, 2310, 2748, 2808
HpySE526I ACGT 3 cut(s) 110, 1357, 2834
Hsp92I GRCGYC 2 cut(s) 371, 2834
HspAI GCGC 1 cut(s) 2904
KpnI GGTACC 2 cut(s) 123, 1557
KroI GCCGGC 2 cut(s) 853, 2900
KroNI GCCGGC 2 cut(s) 855, 2902
Ksp22I TGATCA 1 cut(s) 1663
KspAI GTTAAC 1 cut(s) 2797
Kzo9I GATC 9 cut(s) 224, 454, 600, 1207, 1276, 1384, 1432, 1663, 2554
LguI GCTCTTC 2 cut(s) 512, 2782
LmnI GCTCC 7 cut(s) 40, 736, 1292, 1462, 1600, 2783, 2861
LweI GCATC 5 cut(s) 185, 328, 1420, 2173, 2610
MabI ACCWGGT 2 cut(s) 1248, 1556
MaeI CTAG 4 cut(s) 23, 222, 1302, 1982
MaeII ACGT 3 cut(s) 110, 1357, 2834
MaeIII GTNAC 5 cut(s) 128, 980, 1522, 2830, 3212
MalI GATC 9 cut(s) 226, 456, 602, 1209, 1278, 1386, 1434, 1665, 2556
MboI GATC 9 cut(s) 224, 454, 600, 1207, 1276, 1384, 1432, 1663, 2554
MfeI CAATTG 1 cut(s) 1406
MflI RGATCY 4 cut(s) 224, 454, 1432, 2554
MhlI GDGCHC 3 cut(s) 88, 1597, 2777
MluI ACGCGT 1 cut(s) 125
MlyI GAGTC 4 cut(s) 485, 586, 914, 1812
MmeI TCCRAC 7 cut(s) 432, 662, 1394, 1998, 2117, 2281, 2640
Mph1103I ATGCAT 1 cut(s) 200
MroNI GCCGGC 2 cut(s) 853, 2900
MseI TTAA 9 cut(s) 861, 1224, 1325, 2051, 2700, 2736, 2796, 2802, 3253
MspA1I CMGCKG 2 cut(s) 1220, 3084
MspI CCGG 4 cut(s) 570, 854, 995, 2901
MspR9I CCNGG 7 cut(s) 232, 571, 926, 1250, 1558, 2454, 2634
MunI CAATTG 1 cut(s) 1406
MvaI CCWGG 6 cut(s) 232, 926, 1250, 1558, 2454, 2634
MvnI CGCG 1 cut(s) 127
MwoI GCNNNNNNNGC 8 cut(s) 92, 901, 2702, 2967, 3021, 3072, 3081, 3090
NaeI GCCGGC 2 cut(s) 855, 2902
NciI CCSGG 1 cut(s) 571
NcoI CCATGG 1 cut(s) 183
NdeI CATATG 4 cut(s) 175, 476, 1266, 1375
NdeII GATC 9 cut(s) 224, 454, 600, 1207, 1276, 1384, 1432, 1663, 2554
NgoMIV GCCGGC 2 cut(s) 853, 2900
NlaIV GGNNCC 8 cut(s) 121, 664, 939, 1555, 1596, 2028, 2381, 2764
NmeAIII GCCGAG 1 cut(s) 73
NmuCI GTSAC 3 cut(s) 128, 980, 2830
NsiI ATGCAT 1 cut(s) 200
NspI RCATGY 4 cut(s) 47, 1659, 2068, 2868
PaeI GCATGC 1 cut(s) 47
PagI TCATGA 2 cut(s) 2526, 3196
PaqCI CACCTGC 1 cut(s) 86
PciSI GCTCTTC 2 cut(s) 512, 2782
PdiI GCCGGC 2 cut(s) 855, 2902
PflMI CCANNNNNTGG 3 cut(s) 1536, 1563, 2193
PfoI TCCNGGA 1 cut(s) 230
PleI GAGTC 4 cut(s) 485, 585, 914, 1812
PpsI GAGTC 4 cut(s) 485, 585, 914, 1812
PpuMI RGGWCCY 3 cut(s) 938, 1708, 2227
PsiI TTATAA 1 cut(s) 353
Psp124BI GAGCTC 1 cut(s) 2777
Psp1406I AACGTT 1 cut(s) 110
Psp5II RGGWCCY 3 cut(s) 938, 1708, 2227
Psp6I CCWGG 6 cut(s) 230, 924, 1248, 1556, 2452, 2632
PspFI CCCAGC 1 cut(s) 2168
PspGI CCWGG 6 cut(s) 230, 924, 1248, 1556, 2452, 2632
PspN4I GGNNCC 8 cut(s) 121, 664, 939, 1555, 1596, 2028, 2381, 2764
PspPI GGNCC 8 cut(s) 269, 662, 747, 851, 938, 1708, 2227, 2379
PspPPI RGGWCCY 3 cut(s) 938, 1708, 2227
PstI CTGCAG 1 cut(s) 793
PstNI CAGNNNCTG 3 cut(s) 278, 2633, 3087
PsuI RGATCY 4 cut(s) 224, 454, 1432, 2554
PvuII CAGCTG 1 cut(s) 1220
RsaI GTAC 9 cut(s) 121, 153, 583, 590, 845, 881, 951, 1555, 2329
RsaNI GTAC 9 cut(s) 120, 152, 582, 589, 844, 880, 950, 1554, 2328
SacI GAGCTC 1 cut(s) 2777
SapI GCTCTTC 2 cut(s) 512, 2782
SaqAI TTAA 9 cut(s) 861, 1224, 1325, 2051, 2700, 2736, 2796, 2802, 3253
Sau3AI GATC 9 cut(s) 224, 454, 600, 1207, 1276, 1384, 1432, 1663, 2554
Sau96I GGNCC 8 cut(s) 269, 662, 747, 851, 938, 1708, 2227, 2379
ScaI AGTACT 1 cut(s) 153
SchI GAGTC 4 cut(s) 485, 586, 914, 1812
ScrFI CCNGG 7 cut(s) 232, 571, 926, 1250, 1558, 2454, 2634
SduI GDGCHC 3 cut(s) 88, 1597, 2777
SexAI ACCWGGT 2 cut(s) 1248, 1556
SfaNI GCATC 5 cut(s) 185, 328, 1420, 2173, 2610
SfcI CTRYAG 2 cut(s) 789, 874
SinI GGWCC 6 cut(s) 662, 747, 938, 1708, 2227, 2379
SmlI CTYRAG 2 cut(s) 740, 2770
SmoI CTYRAG 2 cut(s) 740, 2770
SphI GCATGC 1 cut(s) 47
SspMI CTAG 4 cut(s) 23, 222, 1302, 1982
SstI GAGCTC 1 cut(s) 2777
StyD4I CCNGG 7 cut(s) 230, 569, 924, 1248, 1556, 2452, 2632
StyI CCWWGG 3 cut(s) 183, 265, 2285
TaaI ACNGT 7 cut(s) 677, 1016, 1066, 1979, 2533, 2629, 2658
TaiI ACGT 3 cut(s) 113, 1360, 2837
TaqI TCGA 5 cut(s) 322, 1000, 1049, 2131, 2724
TatI WGTACW 3 cut(s) 151, 588, 2327
TauI GCSGC 4 cut(s) 1816, 2970, 3027, 3087
Tru1I TTAA 9 cut(s) 861, 1224, 1325, 2051, 2700, 2736, 2796, 2802, 3253
Tru9I TTAA 9 cut(s) 861, 1224, 1325, 2051, 2700, 2736, 2796, 2802, 3253
TscAI CASTG 6 cut(s) 1069, 1871, 2381, 2577, 2878, 2977
TseFI GTSAC 3 cut(s) 128, 980, 2830
Tsp45I GTSAC 3 cut(s) 128, 980, 2830
TspGWI ACGGA 2 cut(s) 888, 2703
TspRI CASTG 6 cut(s) 1069, 1871, 2381, 2577, 2878, 2977
Van91I CCANNNNNTGG 3 cut(s) 1536, 1563, 2193
VpaK11BI GGWCC 6 cut(s) 662, 747, 938, 1708, 2227, 2379
XagI CCTNNNNNAGG 1 cut(s) 2751
XapI RAATTY 6 cut(s) 30, 1098, 2339, 2398, 2444, 2540
XbaI TCTAGA 1 cut(s) 1301
XceI RCATGY 4 cut(s) 47, 1659, 2068, 2868
XcmI CCANNNNNNNNNTGG 1 cut(s) 1207
XmiI GTMKAC 1 cut(s) 777
XspI CTAG 4 cut(s) 23, 222, 1302, 1982
ZraI GACGTC 1 cut(s) 2835
ZrmI AGTACT 1 cut(s) 153
Zsp2I ATGCAT 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.