Rh2DG222100

Glycine-rich domain-containing protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0002521
Physical Location & Seq
Reverse (-)
21389725 .. 21395684
5960 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG222100.1

Sequence Viewer

Length: 2565 bp
ATGGAGATGGAGATGGAGATGGAGAAGGAACAGGAGCTTGAGTGGACCAAAGCGCAGAGCATTGGAATAAGCGTAGACCTTGTGGCTGCAGCAAAAAAGCAGCTTCAGTTTCTTGCTGCTGTTGATAGGAATCGGTATCTGTACGAAGGGCTGGCTCTTAACAGGGCTATCTATAGGTACAACTCTTGTTGGCTTCCATTGCTTGCCAAACATTCAGACTCCCAGGTTTCTGAGGGACCTTTGGTTGTACCTCTTGATTGTGAATGGATTTGGCATTGTCACAGGCTAAATCCGGTTCGATACAAGACTGACTGTGAAGAACTTTATGGAAAGATTCTTGACAACTCGAATGTTGTTTCCTCTGTTGAAGGTTCTTGTAAAAGTAAAACTGAAGAAATTTGGAATAGTCTGTATCCAGCAGAACCCTACAACTTCAACTTACAAAAGGCATTGTCCGAGGATATATCTGAAAGGATTTCCAAACTTGACAAATGCACCAAATATGATCTGGTTTCAGCTGTTAAAAGACAATCTCCTTTCTTTTACCAGGTATCTCCACCCCATATGAACCATGATCTGTTTCTTGAAGGAGCTGCGTCTAGATATAAAGGGTTTCTTCAGTTAATCAAGAGCAATAATGAGAAGTCTCTACGACGTTTCTGCGTTCCAACATATGATATTGATCTTATCTGGCATACTCACCAATTACATCCTGTATCCTATTGTAAAGATCTGCATGAACTCCTTGGAAAGGTATTGGAGCATGACGACATGGATTCGGACAGAACTAAAGGGAAGAAGCTGGATACTGGGTTCTCTGTAACCACCAAGCAATGGGAAGAGGCATTTGGTACCAGGTATTGGAGAGCAGGGGCAATGTATAGAGGCAGGGCTCCATCTCCTCTCACAACCACTCCTTATCAGTCTAACATCACTAGCAAGGATGTAATTGCATGTCCTGATCATCAGAAAGTAATTGAGCTTCCAGAGGTGAAAGCTGTGGAGGTCCTTTTGGAGTTTCTGGAAGTTAGGAACTTACCAGAAGGACACAAAGGGCTTCTCTTTGCCTCATTCAGTAAGACTACAAAGGATATATTTTTCAATGCTAAGCGGCGACTCAGCATTTTTTCTGAGTTTGGAGAGAAACAGGTTGCTTCTTTCCAGTGTGAACCTACTGGAGAGCTGCTTTTTGAACTCATGTCCCATTCACCTTTATATATACCAATGAAAAGGGCATATAAAACATTAGGTTCTGCTTCGTTCTCTCTTCAAGACTGTCTAGTCCCACCCTCTAAATTATATGCAGAAAAATGGTTAGAGATGGTGCCAAGCTCTAAAGTTGGGAACTTACAACCAATCTACATGCGATTCGCTATGTCTTTTACTATTCCAACCATAGCACAACACACACTTCATGTGGTTCGTTCTCGACCGTTTTCAAAAAGTTCTTGCTTTTTCCCCTTTGCTGGGAAGAATCAAGATGCCAAGAATTGGACACAAGTGATTGATGAAACTGGAACAGAGGTCCTCCGCCTGCAAATGAGGGATACTGAAAAGGAAAAGGTAAAGGGAATCAGCGTTCCAAGGAAAGAGGTGGTTGGAATTACCAAGTCTGGGAAAATATGTACTCTTGCAGAATTTGTGGGGACTGGTTGGTCTTTGATAGATTCACACTGGTCCCTTCATCGTGAGAAAAATTCCGAGGGATACCTCTTTTTGCTAAAGGGAAAAAAGATGGTCAAATTCTTCCCAGGCAGAAAGCTGGATTATGAACATAAGCATTATGAGGAAAATACAACTGAAGATAAAACTGAACAACACTTCGTGACCTTAGTTGAATTCTCTGCTGAAGATCCTTATGGCAAAGCAGTGGCATTGCTTGACCTAAAATCCGGGTGCATCAAGGTAAAGGAAGAGTCAATAACAGTTCCTGGGGTCATAGTGGCTTTTATACTGTCGGATAAGCTAAAGAAGGAAAGATATGACGGGTTTGCTGTTAACCCTGCGGAGATGGGTAGTGTCGAGGAAGAAATTAAAGCGAACCCTGAGGAAGGCAAAGGCACCAACTTGAGCTCTTCTGGAGCAAGTGTAGTTAACCTTAAATCTGAGGTGGTTGAAGGAAATGTAGTGACGTCTCAAAAAGGTGGAGGCTGTGGTGGAGCATGTGGCAGTGGGTGTGGAAATGCAACAAGGAGTGCCGGCGCTGGTGGATGTGGAAGTGGTTGTGGTGGAGGATGCGGAAACATGGAGAAGAGTAGCGCTTGCGGCAGTGGATGTGGTGGAGGATGTGGAAACTTGGTGAAGAGTGGCGGCTGTGGTGGTGGCTGTGGAGGTGGCTGTGGTGGTGGCTGTGGAGGTGGTTGTGGAAACATACTGAAGAGTGGTGGCTGTGGTGGCTGTGGCGGCGGCTGTGGGAACATACTGAAGAGTGGTGGCTGCGGTGGGTGTGGTGGTAGCGGTGGTTGTGGTAGTGGGTGCGGAGGCTTATTCAAGGGTAATGGTTATCATGAAAATACATCTGGTAACTCATGTGCCGAAGACCACCCAAAGGCAGTAGTTGTTTAA

Protein Analysis

854

Amino Acids

93.04

Weight (kDa)

7.41

Isoelectric Point (pI)

43.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRDP-like PF07173 11 - 104 1.1e-09 Glycine-rich domain-containing protein-like
GRDP-like PF07173 100 - 244 7.3e-58 Glycine-rich domain-containing protein-like
C2_GRDP PF25334 331 - 463 1.1e-53 C2 domain of glycine-rich domain-containing protein
GRDP_C PF25335 501 - 636 1.6e-36 Glycine Rich Domain Protein C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1280, 1654
AatII GACGTC 1 cut(s) 2135
Acc65I GGTACC 1 cut(s) 851
AccB1I GGYRCC 3 cut(s) 851, 1324, 2060
AccB7I CCANNNNNTGG 3 cut(s) 834, 861, 1491
AccI GTMKAC 1 cut(s) 75
AclWI GGATC 1 cut(s) 1847
AcsI RAATTY 5 cut(s) 396, 1637, 1696, 1742, 1838
AcuI CTGAAG 7 cut(s) 89, 411, 602, 1821, 1869, 2396, 2444
AcyI GRCGYC 1 cut(s) 2132
AdeI CACNNNGTG 1 cut(s) 1825
AfaI GTAC 5 cut(s) 143, 179, 249, 853, 1627
AfeI AGCGCT 1 cut(s) 2260
AfiI CCNNNNNNNGG 9 cut(s) 751, 834, 861, 1466, 1467, 1491, 1614, 2051, 2548
AjnI CCWGG 5 cut(s) 222, 546, 854, 1750, 1930
AjuI GAANNNNNNNTTGG 2 cut(s) 831, 863
AloI GAACNNNNNNTCC 4 cut(s) 797, 829, 1563, 1595
Alw21I GWGCWC 1 cut(s) 2075
Alw26I GTCTC 2 cut(s) 651, 2139
AlwI GGATC 1 cut(s) 1847
AlwNI CAGNNNCTG 1 cut(s) 1931
Aor51HI AGCGCT 1 cut(s) 2260
ApeKI GCWGC 7 cut(s) 86, 89, 100, 116, 593, 1183, 2436
ApoI RAATTY 5 cut(s) 396, 1637, 1696, 1742, 1838
Asp718I GGTACC 1 cut(s) 851
AspLEI GCGC 3 cut(s) 55, 2204, 2261
AspS9I GGNCC 5 cut(s) 45, 236, 1006, 1525, 1677
AsuC2I CCSGG 1 cut(s) 1894
AsuHPI GGTGA 4 cut(s) 692, 1003, 1200, 2311
AvaII GGWCC 5 cut(s) 45, 236, 1006, 1525, 1677
AxyI CCTNAGG 1 cut(s) 2046
BanI GGYRCC 3 cut(s) 851, 1324, 2060
BanII GRGCYC 2 cut(s) 895, 2075
BarI GAAGNNNNNNTAC 2 cut(s) 966, 998
BbsI GAAGAC 1 cut(s) 2544
Bbv12I GWGCWC 1 cut(s) 2075
BbvI GCAGC 7 cut(s) 73, 101, 103, 112, 580, 1170, 2423
BccI CCATC 6 cut(s) 7, 13, 904, 1315, 1729, 2005
BcgI CGANNNNNNTGC 2 cut(s) 642, 676
BciT130I CCWGG 5 cut(s) 224, 548, 856, 1752, 1932
BciVI GTATCC 5 cut(s) 423, 727, 799, 1540, 1700
BclI TGATCA 1 cut(s) 961
BcnI CCSGG 1 cut(s) 1894
BcoDI GTCTC 2 cut(s) 651, 2139
BfaI CTAG 3 cut(s) 600, 936, 1280
BfmI CTRYAG 2 cut(s) 87, 172
BfoI RGCGCY 2 cut(s) 2205, 2262
BfuI GTATCC 5 cut(s) 423, 727, 799, 1540, 1700
BglII AGATCT 1 cut(s) 730
BlpI GCTNAGC 1 cut(s) 1107
Bme1390I CCNGG 6 cut(s) 224, 548, 856, 1752, 1894, 1932
Bme18I GGWCC 5 cut(s) 45, 236, 1006, 1525, 1677
BmgT120I GGNCC 5 cut(s) 45, 236, 1006, 1525, 1677
BmiI GGNNCC 6 cut(s) 237, 853, 894, 1326, 1679, 2062
BmrFI CCNGG 6 cut(s) 224, 548, 856, 1752, 1894, 1932
BmrI ACTGGG 1 cut(s) 819
BmsI GCATC 3 cut(s) 1471, 1908, 2225
BmuI ACTGGG 1 cut(s) 819
BpiI GAAGAC 1 cut(s) 2544
BpmI CTGGAG 2 cut(s) 1197, 2100
Bpu1102I GCTNAGC 1 cut(s) 1107
BpuEI CTTGAG 2 cut(s) 59, 2089
BpuMI CCSGG 1 cut(s) 1894
BsaBI GATNNNNATC 2 cut(s) 129, 681
BsaHI GRCGYC 1 cut(s) 2132
BsaJI CCNNGG 7 cut(s) 222, 456, 745, 1583, 1701, 1750, 1931
BsaWI WCCGGW 1 cut(s) 292
BsaXI ACNNNNNCTCC 6 cut(s) 26, 56, 898, 928, 2073, 2103
Bsc4I CCNNNNNNNGG 9 cut(s) 751, 834, 861, 1466, 1467, 1491, 1614, 2051, 2548
Bse118I RCCGGY 1 cut(s) 2198
Bse1I ACTGG 6 cut(s) 814, 1162, 1180, 1519, 1654, 1679
Bse21I CCTNAGG 1 cut(s) 2046
Bse3DI GCAATG 4 cut(s) 197, 839, 882, 1874
Bse8I GATNNNNATC 2 cut(s) 129, 681
BseBI CCWGG 5 cut(s) 224, 548, 856, 1752, 1932
BseDI CCNNGG 7 cut(s) 222, 456, 745, 1583, 1701, 1750, 1931
BseGI GGATG 6 cut(s) 709, 949, 2216, 2240, 2279, 2291
BseJI GATNNNNATC 2 cut(s) 129, 681
BseLI CCNNNNNNNGG 9 cut(s) 751, 834, 861, 1466, 1467, 1491, 1614, 2051, 2548
BseMI GCAATG 4 cut(s) 197, 839, 882, 1874
BseMII CTCAG 5 cut(s) 222, 1122, 1132, 2037, 2097
BseNI ACTGG 6 cut(s) 814, 1162, 1180, 1519, 1654, 1679
BseRI GAGGAG 1 cut(s) 891
BseXI GCAGC 7 cut(s) 73, 101, 103, 112, 580, 1170, 2423
BseYI CCCAGC 1 cut(s) 1466
Bsh1285I CGRYCG 1 cut(s) 1433
BshNI GGYRCC 3 cut(s) 851, 1324, 2060
BsiEI CGRYCG 1 cut(s) 1433
BsiHKAI GWGCWC 1 cut(s) 2075
BsiSI CCGG 3 cut(s) 293, 1893, 2199
BslFI GGGAC 5 cut(s) 249, 1186, 1268, 1660, 1663
BslI CCNNNNNNNGG 9 cut(s) 751, 834, 861, 1466, 1467, 1491, 1614, 2051, 2548
BsmAI GTCTC 2 cut(s) 651, 2139
BsmBI CGTCTC 1 cut(s) 2139
BsmFI GGGAC 5 cut(s) 249, 1186, 1268, 1660, 1663
Bsp1286I GDGCHC 2 cut(s) 895, 2075
Bsp143I GATC 6 cut(s) 505, 574, 682, 730, 961, 1852
Bsp1720I GCTNAGC 1 cut(s) 1107
BspCNI CTCAG 5 cut(s) 223, 1123, 1131, 2038, 2098
BspHI TCATGA 1 cut(s) 2506
BspLI GGNNCC 6 cut(s) 237, 853, 894, 1326, 1679, 2062
BspMAI CTGCAG 1 cut(s) 91
BspPI GGATC 1 cut(s) 1847
BspQI GCTCTTC 1 cut(s) 2080
BspT107I GGYRCC 3 cut(s) 851, 1324, 2060
BsrDI GCAATG 4 cut(s) 197, 839, 882, 1874
BsrFI RCCGGY 1 cut(s) 2198
BsrI ACTGG 6 cut(s) 814, 1162, 1180, 1519, 1654, 1679
BssAI RCCGGY 1 cut(s) 2198
BssECI CCNNGG 7 cut(s) 222, 456, 745, 1583, 1701, 1750, 1931
BssMI GATC 6 cut(s) 505, 574, 682, 730, 961, 1852
BssNI GRCGYC 1 cut(s) 2132
BssT1I CCWWGG 2 cut(s) 745, 1583
Bst2UI CCWGG 5 cut(s) 224, 548, 856, 1752, 1932
Bst4CI ACNGT 5 cut(s) 314, 1277, 1434, 1927, 1956
Bst6I CTCTTC 8 cut(s) 834, 1272, 1908, 2080, 2246, 2297, 2372, 2420
BstACI GRCGYC 1 cut(s) 2132
BstC8I GCNNGC 5 cut(s) 153, 204, 1535, 2200, 2263
BstDEI CTNAG 7 cut(s) 231, 1107, 1118, 1131, 1831, 2046, 2106
BstENI CCTNNNNNAGG 2 cut(s) 749, 2049
BstF5I GGATG 6 cut(s) 709, 949, 2216, 2240, 2279, 2291
BstH2I RGCGCY 2 cut(s) 2205, 2262
BstHHI GCGC 3 cut(s) 55, 2204, 2261
BstKTI GATC 6 cut(s) 508, 577, 685, 733, 964, 1855
BstMAI GTCTC 2 cut(s) 651, 2139
BstMBI GATC 6 cut(s) 505, 574, 682, 730, 961, 1852
BstMCI CGRYCG 1 cut(s) 1433
BstMWI GCNNNNNNNGC 4 cut(s) 199, 2265, 2394, 2403
BstNI CCWGG 5 cut(s) 224, 548, 856, 1752, 1932
BstNSI RCATGY 3 cut(s) 957, 1366, 2166
BstSCI CCNGG 6 cut(s) 222, 546, 854, 1750, 1892, 1930
BstSFI CTRYAG 2 cut(s) 87, 172
BstV1I GCAGC 7 cut(s) 73, 101, 103, 112, 580, 1170, 2423
BstV2I GAAGAC 1 cut(s) 2544
BstX2I RGATCY 2 cut(s) 730, 1852
BstYI RGATCY 2 cut(s) 730, 1852
Bsu36I CCTNAGG 1 cut(s) 2046
BsuI GTATCC 5 cut(s) 423, 727, 799, 1540, 1700
BtsCI GGATG 6 cut(s) 709, 949, 2216, 2240, 2279, 2291
BtsI GCAGTG 3 cut(s) 1875, 2176, 2275
BtsIMutI CAGTG 5 cut(s) 1169, 1672, 1875, 2176, 2275
Cac8I GCNNGC 5 cut(s) 153, 204, 1535, 2200, 2263
CaiI CAGNNNCTG 1 cut(s) 1931
CciI TCATGA 1 cut(s) 2506
CfoI GCGC 3 cut(s) 55, 2204, 2261
Cfr10I RCCGGY 1 cut(s) 2198
Cfr13I GGNCC 5 cut(s) 45, 236, 1006, 1525, 1677
CseI GACGC 1 cut(s) 585
CsiI ACCWGGT 2 cut(s) 546, 854
Csp6I GTAC 5 cut(s) 142, 178, 248, 852, 1626
CviQI GTAC 5 cut(s) 142, 178, 248, 852, 1626
DdeI CTNAG 7 cut(s) 231, 1107, 1118, 1131, 1831, 2046, 2106
DpnI GATC 6 cut(s) 507, 576, 684, 732, 963, 1854
DpnII GATC 6 cut(s) 505, 574, 682, 730, 961, 1852
DraIII CACNNNGTG 1 cut(s) 1825
DrdI GACNNNNNNGTC 2 cut(s) 1280, 1654
DseDI GACNNNNNNGTC 2 cut(s) 1280, 1654
Eam1104I CTCTTC 8 cut(s) 834, 1272, 1908, 2080, 2246, 2297, 2372, 2420
EarI CTCTTC 8 cut(s) 834, 1272, 1908, 2080, 2246, 2297, 2372, 2420
EciI GGCGGA 1 cut(s) 1520
Ecl136II GAGCTC 1 cut(s) 2073
Eco130I CCWWGG 2 cut(s) 745, 1583
Eco24I GRGCYC 2 cut(s) 895, 2075
Eco47I GGWCC 5 cut(s) 45, 236, 1006, 1525, 1677
Eco47III AGCGCT 1 cut(s) 2260
Eco53kI GAGCTC 1 cut(s) 2073
Eco57I CTGAAG 7 cut(s) 89, 411, 602, 1821, 1869, 2396, 2444
Eco81I CCTNAGG 1 cut(s) 2046
EcoICRI GAGCTC 1 cut(s) 2073
EcoNI CCTNNNNNAGG 2 cut(s) 749, 2049
EcoO109I RGGNCCY 3 cut(s) 236, 1006, 1525
EcoRI GAATTC 1 cut(s) 1838
EcoRII CCWGG 5 cut(s) 222, 546, 854, 1750, 1930
EcoT14I CCWWGG 2 cut(s) 745, 1583
EcoT38I GRGCYC 2 cut(s) 895, 2075
ErhI CCWWGG 2 cut(s) 745, 1583
Esp3I CGTCTC 1 cut(s) 2139
FalI AAGNNNNNCTT 2 cut(s) 600, 632
FaqI GGGAC 5 cut(s) 249, 1186, 1268, 1660, 1663
FauNDI CATATG 2 cut(s) 564, 673
FbaI TGATCA 1 cut(s) 961
FblI GTMKAC 1 cut(s) 75
FokI GGATG 6 cut(s) 696, 956, 2223, 2247, 2286, 2298
FriOI GRGCYC 2 cut(s) 895, 2075
FspBI CTAG 3 cut(s) 600, 936, 1280
GlaI GCGC 3 cut(s) 54, 2203, 2260
GsaI CCCAGC 1 cut(s) 1470
GsuI CTGGAG 2 cut(s) 1197, 2100
HaeII RGCGCY 2 cut(s) 2205, 2262
HapII CCGG 3 cut(s) 293, 1893, 2199
HgaI GACGC 1 cut(s) 585
HhaI GCGC 3 cut(s) 55, 2204, 2261
Hin1I GRCGYC 1 cut(s) 2132
Hin6I GCGC 3 cut(s) 53, 2202, 2259
HinP1I GCGC 3 cut(s) 53, 2202, 2259
HincII GTYRAC 2 cut(s) 1999, 2095
HindII GTYRAC 2 cut(s) 1999, 2095
HpaI GTTAAC 2 cut(s) 1999, 2095
HpaII CCGG 3 cut(s) 293, 1893, 2199
HphI GGTGA 4 cut(s) 692, 1003, 1200, 2311
Hpy166II GTNNAC 5 cut(s) 45, 76, 1169, 1999, 2095
Hpy8I GTNNAC 5 cut(s) 45, 76, 1169, 1999, 2095
Hpy99I CGWCG 1 cut(s) 657
HpyAV CCTTC 9 cut(s) 19, 140, 362, 581, 1037, 1691, 1966, 2045, 2111
HpyCH4III ACNGT 5 cut(s) 314, 1277, 1434, 1927, 1956
HpyCH4IV ACGT 2 cut(s) 655, 2132
HpyCH4V TGCA 9 cut(s) 89, 495, 736, 953, 1304, 1537, 1634, 1899, 2186
HpyF10VI GCNNNNNNNGC 4 cut(s) 199, 2265, 2394, 2403
HpyF3I CTNAG 7 cut(s) 231, 1107, 1118, 1131, 1831, 2046, 2106
HpySE526I ACGT 2 cut(s) 655, 2132
Hsp92I GRCGYC 1 cut(s) 2132
HspAI GCGC 3 cut(s) 53, 2202, 2259
KpnI GGTACC 1 cut(s) 855
KroI GCCGGC 1 cut(s) 2198
KroNI GCCGGC 1 cut(s) 2200
Ksp22I TGATCA 1 cut(s) 961
KspAI GTTAAC 2 cut(s) 1999, 2095
Kzo9I GATC 6 cut(s) 505, 574, 682, 730, 961, 1852
LguI GCTCTTC 1 cut(s) 2080
LmnI GCTCC 6 cut(s) 34, 590, 760, 898, 2081, 2159
Lsp1109I GCAGC 7 cut(s) 73, 101, 103, 112, 580, 1170, 2423
LweI GCATC 3 cut(s) 1471, 1908, 2225
MabI ACCWGGT 2 cut(s) 546, 854
MaeI CTAG 3 cut(s) 600, 936, 1280
MaeII ACGT 2 cut(s) 655, 2132
MaeIII GTNAC 5 cut(s) 278, 820, 1825, 2128, 2522
MalI GATC 6 cut(s) 507, 576, 684, 732, 963, 1854
MboI GATC 6 cut(s) 505, 574, 682, 730, 961, 1852
MflI RGATCY 2 cut(s) 730, 1852
MhlI GDGCHC 2 cut(s) 895, 2075
MlyI GAGTC 3 cut(s) 212, 1110, 1925
MmeI TCCRAC 4 cut(s) 692, 1415, 1579, 1938
MroNI GCCGGC 1 cut(s) 2198
MseI TTAA 8 cut(s) 159, 522, 623, 1998, 2034, 2094, 2100, 2563
MspA1I CMGCKG 1 cut(s) 518
MspI CCGG 3 cut(s) 293, 1893, 2199
MspR9I CCNGG 6 cut(s) 224, 548, 856, 1752, 1894, 1932
MvaI CCWGG 5 cut(s) 224, 548, 856, 1752, 1932
MwoI GCNNNNNNNGC 4 cut(s) 199, 2265, 2394, 2403
NaeI GCCGGC 1 cut(s) 2200
NciI CCSGG 1 cut(s) 1894
NdeI CATATG 2 cut(s) 564, 673
NdeII GATC 6 cut(s) 505, 574, 682, 730, 961, 1852
NgoMIV GCCGGC 1 cut(s) 2198
NlaIV GGNNCC 6 cut(s) 237, 853, 894, 1326, 1679, 2062
NmuCI GTSAC 3 cut(s) 278, 1825, 2128
NspI RCATGY 3 cut(s) 957, 1366, 2166
PagI TCATGA 1 cut(s) 2506
PciSI GCTCTTC 1 cut(s) 2080
PdiI GCCGGC 1 cut(s) 2200
PfeI GAWTC 7 cut(s) 130, 334, 776, 1368, 1474, 1572, 1667
PflMI CCANNNNNTGG 3 cut(s) 834, 861, 1491
PleI GAGTC 3 cut(s) 212, 1110, 1924
PpsI GAGTC 3 cut(s) 212, 1110, 1924
PpuMI RGGWCCY 3 cut(s) 236, 1006, 1525
Psp124BI GAGCTC 1 cut(s) 2075
Psp5II RGGWCCY 3 cut(s) 236, 1006, 1525
Psp6I CCWGG 5 cut(s) 222, 546, 854, 1750, 1930
PspFI CCCAGC 1 cut(s) 1466
PspGI CCWGG 5 cut(s) 222, 546, 854, 1750, 1930
PspN4I GGNNCC 6 cut(s) 237, 853, 894, 1326, 1679, 2062
PspPI GGNCC 5 cut(s) 45, 236, 1006, 1525, 1677
PspPPI RGGWCCY 3 cut(s) 236, 1006, 1525
PstI CTGCAG 1 cut(s) 91
PstNI CAGNNNCTG 1 cut(s) 1931
PsuI RGATCY 2 cut(s) 730, 1852
PvuII CAGCTG 1 cut(s) 518
RsaI GTAC 5 cut(s) 143, 179, 249, 853, 1627
RsaNI GTAC 5 cut(s) 142, 178, 248, 852, 1626
SacI GAGCTC 1 cut(s) 2075
SapI GCTCTTC 1 cut(s) 2080
SaqAI TTAA 8 cut(s) 159, 522, 623, 1998, 2034, 2094, 2100, 2563
Sau3AI GATC 6 cut(s) 505, 574, 682, 730, 961, 1852
Sau96I GGNCC 5 cut(s) 45, 236, 1006, 1525, 1677
SchI GAGTC 3 cut(s) 212, 1110, 1925
ScrFI CCNGG 6 cut(s) 224, 548, 856, 1752, 1894, 1932
SduI GDGCHC 2 cut(s) 895, 2075
SexAI ACCWGGT 2 cut(s) 546, 854
SfaNI GCATC 3 cut(s) 1471, 1908, 2225
SfcI CTRYAG 2 cut(s) 87, 172
SinI GGWCC 5 cut(s) 45, 236, 1006, 1525, 1677
SmlI CTYRAG 2 cut(s) 38, 2068
SmoI CTYRAG 2 cut(s) 38, 2068
SspMI CTAG 3 cut(s) 600, 936, 1280
SstI GAGCTC 1 cut(s) 2075
StyD4I CCNGG 6 cut(s) 222, 546, 854, 1750, 1892, 1930
StyI CCWWGG 2 cut(s) 745, 1583
TaaI ACNGT 5 cut(s) 314, 1277, 1434, 1927, 1956
TaiI ACGT 2 cut(s) 658, 2135
TaqI TCGA 4 cut(s) 298, 347, 1429, 2022
TatI WGTACW 1 cut(s) 1625
TauI GCSGC 5 cut(s) 1114, 2268, 2313, 2406, 2409
TfiI GAWTC 7 cut(s) 130, 334, 776, 1368, 1474, 1572, 1667
Tru1I TTAA 8 cut(s) 159, 522, 623, 1998, 2034, 2094, 2100, 2563
Tru9I TTAA 8 cut(s) 159, 522, 623, 1998, 2034, 2094, 2100, 2563
TscAI CASTG 5 cut(s) 1169, 1679, 1875, 2176, 2275
TseFI GTSAC 3 cut(s) 278, 1825, 2128
TseI GCWGC 7 cut(s) 86, 89, 100, 116, 593, 1183, 2436
Tsp45I GTSAC 3 cut(s) 278, 1825, 2128
TspDTI ATGAA 8 cut(s) 581, 753, 1241, 1403, 1524, 1673, 1785, 2523
TspRI CASTG 5 cut(s) 1169, 1679, 1875, 2176, 2275
Van91I CCANNNNNTGG 3 cut(s) 834, 861, 1491
VpaK11BI GGWCC 5 cut(s) 45, 236, 1006, 1525, 1677
XagI CCTNNNNNAGG 2 cut(s) 749, 2049
XapI RAATTY 5 cut(s) 396, 1637, 1696, 1742, 1838
XbaI TCTAGA 1 cut(s) 599
XceI RCATGY 3 cut(s) 957, 1366, 2166
XcmI CCANNNNNNNNNTGG 1 cut(s) 505
XmiI GTMKAC 1 cut(s) 75
XspI CTAG 3 cut(s) 600, 936, 1280
ZraI GACGTC 1 cut(s) 2133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.