pycom08g18010

PB1 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0002525
Physical Location & Seq
Forward (+)
18238769 .. 18240016
1248 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g18010.2

Sequence Viewer

Length: 1062 bp
ATGATGATCGAGTACGACCGGCTCTACCGCGCTTCCGCTAAGCCCGCCAGGCTTCGACTCTTCCTCTTCCCTCTTGGCACCAACCCTTCCGCTAGCTTCGGCTCCAGCGAGACCAAGTCCGAGCGCCAGTGGTTCGTCGACGCTTTGAATTCCGTTCAGATCGGGTCTCCCGATGGCTCCTCTCCGACGGCTCCCGTCACGTCCTCGCCGACGAACCCCGATTTCTTGTTCGGATTCGATAAGGGATACAAGGCTGTGCCGGTTCCGAAATTTCCCGACTCTGTCACTCCGCCTACGGTTCCCGATTCGCTTGTGAAAAGCACTCTGGTTGGATCTGAGGATCGCCACACCGGAGAACCTGTGATATCTCCGGCGGAGATTCAGAGGCAGATCCAGGAGTTGCAGAGGTTGCAAATCGCTGCCAGTCACGAGCCGACCGCCATATATCACCGGAAAAGCGACGAAATGGGAAACCCTAGGGGTTACACCGGCGATTATTACACGCAAAAACTTCCAGACAACATTCAACCGGCGCAAGGATCGTCTCCGGTGGCAATGCCACCGCCTATGCAATTTTCAACTGCGTATATGCCAGAAAGGCACATGAACACTGGGGGTTATCCGGTGACGGCTGCCCCATCTGGACCGGAGCAGCCCGTTTATCTCATTTCGACTCCGACCGGTGTGTACCAGGCTCCCGCTCCTAGACCGGCAACTGGACCAGTTGGTCAGGCATACTATGGAGTACCGCAGAGGATGGCACCAGAGGTGTACCGGGACGCTCAGGTGTACAATAATGCAGTGGCAGCACAGCAAGCACAACAAATTCAGCAAGCACAACAAGGTCAGCAAATTGCAGCTTATAGTGAAGGGTTTGCACCGGTTGGGTATGACGCCGTCGGGAGGCAGGTATACTACACTGCAGCTGGAGGTGTCGTTTCTTCATACCAGACGGTGGCTCCGGTGGCTGTAGATGCAAGACAAGCTGCTGGTGGTGTATTGAATCAAATTCAAGACGGTAAAGTTGTTGTTTCCATGGCACCCCAAGCGTCTGCTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

354

Amino Acids

37.83

Weight (kDa)

5.84

Isoelectric Point (pI)

53.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 726
Acc36I ACCTGC 1 cut(s) 898
AccB1I GGYRCC 3 cut(s) 77, 760, 1039
AccB7I CCANNNNNTGG 1 cut(s) 955
AccBSI CCGCTC 1 cut(s) 701
AccI GTMKAC 2 cut(s) 138, 912
AccII CGCG 1 cut(s) 30
AclWI GGATC 4 cut(s) 340, 348, 385, 547
AcsI RAATTY 4 cut(s) 148, 269, 825, 1008
AcyI GRCGYC 1 cut(s) 894
AfaI GTAC 5 cut(s) 14, 689, 747, 773, 791
AfiI CCNNNNNNNGG 4 cut(s) 536, 716, 903, 955
AgeI ACCGGT 2 cut(s) 680, 880
AgsI TTSAA 5 cut(s) 148, 527, 579, 1003, 1013
AjiI CACGTC 1 cut(s) 201
AjnI CCWGG 3 cut(s) 47, 393, 690
AluBI AGCT 4 cut(s) 96, 860, 926, 986
AluI AGCT 4 cut(s) 96, 860, 926, 986
Alw26I GTCTC 3 cut(s) 104, 171, 549
AlwI GGATC 4 cut(s) 340, 348, 385, 547
ApeKI GCWGC 7 cut(s) 419, 632, 652, 806, 857, 923, 986
ApoI RAATTY 4 cut(s) 148, 269, 825, 1008
AsiGI ACCGGT 2 cut(s) 680, 880
AspA2I CCTAGG 1 cut(s) 476
AspLEI GCGC 3 cut(s) 32, 126, 535
AspS9I GGNCC 2 cut(s) 644, 719
AsuC2I CCSGG 1 cut(s) 776
AsuHPI GGTGA 2 cut(s) 440, 637
AsuNHI GCTAGC 1 cut(s) 92
AvaII GGWCC 2 cut(s) 644, 719
AvrII CCTAGG 1 cut(s) 476
BanI GGYRCC 3 cut(s) 77, 760, 1039
BauI CACGAG 1 cut(s) 428
BbvI GCAGC 7 cut(s) 406, 619, 664, 818, 869, 935, 973
BccI CCATC 3 cut(s) 167, 646, 751
BceAI ACGGC 3 cut(s) 204, 645, 881
BciT130I CCWGG 3 cut(s) 49, 395, 692
BciVI GTATCC 1 cut(s) 239
BcnI CCSGG 1 cut(s) 776
BcoDI GTCTC 3 cut(s) 104, 171, 549
BfaI CTAG 3 cut(s) 93, 477, 705
BfmI CTRYAG 2 cut(s) 921, 969
BfoI RGCGCY 1 cut(s) 127
BfuAI ACCTGC 1 cut(s) 898
BfuI GTATCC 1 cut(s) 239
BglI GCCNNNNNGGC 2 cut(s) 49, 598
BisI GCNGC 7 cut(s) 420, 633, 653, 807, 858, 924, 987
BlnI CCTAGG 1 cut(s) 476
BlpI GCTNAGC 1 cut(s) 39
BlsI GCNGC 7 cut(s) 421, 634, 654, 808, 859, 925, 988
Bme1390I CCNGG 4 cut(s) 49, 395, 692, 776
Bme18I GGWCC 2 cut(s) 644, 719
BmgBI CACGTC 1 cut(s) 201
BmgT120I GGNCC 2 cut(s) 644, 719
BmrFI CCNGG 4 cut(s) 49, 395, 692, 776
BmrI ACTGGG 1 cut(s) 621
BmsI GCATC 1 cut(s) 964
BmtI GCTAGC 1 cut(s) 96
BmuI ACTGGG 1 cut(s) 621
BpmI CTGGAG 2 cut(s) 88, 948
Bpu10I CCTNAGC 1 cut(s) 783
Bpu1102I GCTNAGC 1 cut(s) 39
BpuMI CCSGG 1 cut(s) 776
BsaHI GRCGYC 1 cut(s) 894
BsaI GGTCTC 2 cut(s) 104, 171
BsaJI CCNNGG 2 cut(s) 476, 1035
BsaWI WCCGGW 8 cut(s) 350, 450, 547, 622, 646, 680, 880, 961
BsaXI ACNNNNNCTCC 6 cut(s) 345, 375, 921, 943, 951, 973
Bsc4I CCNNNNNNNGG 4 cut(s) 536, 716, 903, 955
Bse118I RCCGGY 7 cut(s) 18, 259, 488, 529, 680, 709, 880
Bse1I ACTGG 5 cut(s) 127, 423, 616, 721, 722
Bse3DI GCAATG 1 cut(s) 561
BseBI CCWGG 3 cut(s) 49, 395, 692
BseDI CCNNGG 2 cut(s) 476, 1035
BseGI GGATG 1 cut(s) 762
BseLI CCNNNNNNNGG 4 cut(s) 536, 716, 903, 955
BseMI GCAATG 1 cut(s) 561
BseMII CTCAG 2 cut(s) 327, 797
BseNI ACTGG 5 cut(s) 127, 423, 616, 721, 722
BseRI GAGGAG 1 cut(s) 169
BseXI GCAGC 7 cut(s) 406, 619, 664, 818, 869, 935, 973
Bsh1236I CGCG 1 cut(s) 30
Bsh1285I CGRYCG 3 cut(s) 19, 438, 681
BshNI GGYRCC 3 cut(s) 77, 760, 1039
BshTI ACCGGT 2 cut(s) 680, 880
BsiEI CGRYCG 3 cut(s) 19, 438, 681
BslFI GGGAC 1 cut(s) 791
BslI CCNNNNNNNGG 4 cut(s) 536, 716, 903, 955
BsmAI GTCTC 3 cut(s) 104, 171, 549
BsmBI CGTCTC 1 cut(s) 549
BsmFI GGGAC 1 cut(s) 791
Bso31I GGTCTC 2 cut(s) 104, 171
Bsp1407I TGTACA 1 cut(s) 789
Bsp143I GATC 6 cut(s) 6, 159, 332, 340, 390, 539
Bsp1720I GCTNAGC 1 cut(s) 39
Bsp19I CCATGG 1 cut(s) 1035
BspCNI CTCAG 2 cut(s) 328, 796
BspFNI CGCG 1 cut(s) 30
BspMAI CTGCAG 1 cut(s) 925
BspMI ACCTGC 1 cut(s) 898
BspOI GCTAGC 1 cut(s) 96
BspPI GGATC 4 cut(s) 340, 348, 385, 547
BspT107I GGYRCC 3 cut(s) 77, 760, 1039
BspTNI GGTCTC 2 cut(s) 104, 171
BsrBI CCGCTC 1 cut(s) 701
BsrDI GCAATG 1 cut(s) 561
BsrFI RCCGGY 7 cut(s) 18, 259, 488, 529, 680, 709, 880
BsrGI TGTACA 1 cut(s) 789
BsrI ACTGG 5 cut(s) 127, 423, 616, 721, 722
BssAI RCCGGY 7 cut(s) 18, 259, 488, 529, 680, 709, 880
BssECI CCNNGG 2 cut(s) 476, 1035
BssMI GATC 6 cut(s) 6, 159, 332, 340, 390, 539
BssNAI GTATAC 1 cut(s) 913
BssNI GRCGYC 1 cut(s) 894
BssSI CACGAG 1 cut(s) 428
BssT1I CCWWGG 2 cut(s) 476, 1035
Bst1107I GTATAC 1 cut(s) 913
Bst2BI CACGAG 1 cut(s) 428
Bst2UI CCWGG 3 cut(s) 49, 395, 692
Bst4CI ACNGT 3 cut(s) 298, 955, 1019
Bst6I CTCTTC 2 cut(s) 65, 71
BstACI GRCGYC 1 cut(s) 894
BstAPI GCANNNNNTGC 1 cut(s) 409
BstAUI TGTACA 1 cut(s) 789
BstC8I GCNNGC 4 cut(s) 45, 94, 816, 834
BstDEI CTNAG 3 cut(s) 39, 336, 783
BstDSI CCRYGG 1 cut(s) 1035
BstF5I GGATG 1 cut(s) 762
BstFNI CGCG 1 cut(s) 30
BstH2I RGCGCY 1 cut(s) 127
BstHHI GCGC 3 cut(s) 32, 126, 535
BstKTI GATC 6 cut(s) 9, 162, 335, 343, 393, 542
BstMAI GTCTC 3 cut(s) 104, 171, 549
BstMBI GATC 6 cut(s) 6, 159, 332, 340, 390, 539
BstMCI CGRYCG 3 cut(s) 19, 438, 681
BstNI CCWGG 3 cut(s) 49, 395, 692
BstSCI CCNGG 4 cut(s) 47, 393, 690, 774
BstSFI CTRYAG 2 cut(s) 921, 969
BstUI CGCG 1 cut(s) 30
BstV1I GCAGC 7 cut(s) 406, 619, 664, 818, 869, 935, 973
BstX2I RGATCY 2 cut(s) 332, 390
BstYI RGATCY 2 cut(s) 332, 390
BstZ17I GTATAC 1 cut(s) 913
BsuI GTATCC 1 cut(s) 239
BtgI CCRYGG 1 cut(s) 1035
BtrI CACGTC 1 cut(s) 201
BtsCI GGATG 1 cut(s) 762
BtsI GCAGTG 2 cut(s) 807, 918
BtsIMutI CAGTG 4 cut(s) 134, 609, 807, 918
BveI ACCTGC 1 cut(s) 898
Cac8I GCNNGC 4 cut(s) 45, 94, 816, 834
CfoI GCGC 3 cut(s) 32, 126, 535
Cfr10I RCCGGY 7 cut(s) 18, 259, 488, 529, 680, 709, 880
Cfr13I GGNCC 2 cut(s) 644, 719
CseI GACGC 4 cut(s) 149, 788, 902, 1038
Csp6I GTAC 5 cut(s) 13, 688, 746, 772, 790
CspAI ACCGGT 2 cut(s) 680, 880
CviAII CATG 2 cut(s) 604, 1036
CviQI GTAC 5 cut(s) 13, 688, 746, 772, 790
DdeI CTNAG 3 cut(s) 39, 336, 783
DpnI GATC 6 cut(s) 8, 161, 334, 342, 392, 541
DpnII GATC 6 cut(s) 6, 159, 332, 340, 390, 539
DrdI GACNNNNNNGTC 1 cut(s) 726
DseDI GACNNNNNNGTC 1 cut(s) 726
Eam1104I CTCTTC 2 cut(s) 65, 71
EarI CTCTTC 2 cut(s) 65, 71
EciI GGCGGA 2 cut(s) 279, 389
Eco130I CCWWGG 2 cut(s) 476, 1035
Eco31I GGTCTC 2 cut(s) 104, 171
Eco32I GATATC 1 cut(s) 366
Eco47I GGWCC 2 cut(s) 644, 719
EcoRI GAATTC 1 cut(s) 148
EcoRII CCWGG 3 cut(s) 47, 393, 690
EcoRV GATATC 1 cut(s) 366
EcoT14I CCWWGG 2 cut(s) 476, 1035
ErhI CCWWGG 2 cut(s) 476, 1035
Esp3I CGTCTC 1 cut(s) 549
FaeI CATG 2 cut(s) 607, 1039
FaqI GGGAC 1 cut(s) 791
FatI CATG 2 cut(s) 603, 1035
FauI CCCGC 2 cut(s) 52, 706
FblI GTMKAC 2 cut(s) 138, 912
Fnu4HI GCNGC 7 cut(s) 420, 633, 653, 807, 858, 924, 987
FokI GGATG 1 cut(s) 769
Fsp4HI GCNGC 7 cut(s) 420, 633, 653, 807, 858, 924, 987
FspBI CTAG 3 cut(s) 93, 477, 705
GlaI GCGC 3 cut(s) 31, 125, 534
GluI GCNGC 7 cut(s) 420, 633, 653, 807, 858, 924, 987
GsuI CTGGAG 2 cut(s) 88, 948
HaeII RGCGCY 1 cut(s) 127
HgaI GACGC 4 cut(s) 149, 788, 902, 1038
HhaI GCGC 3 cut(s) 32, 126, 535
Hin1I GRCGYC 1 cut(s) 894
Hin1II CATG 2 cut(s) 607, 1039
Hin6I GCGC 3 cut(s) 30, 124, 533
HinP1I GCGC 3 cut(s) 30, 124, 533
HincII GTYRAC 1 cut(s) 139
HindII GTYRAC 1 cut(s) 139
HinfI GANTC 7 cut(s) 57, 234, 278, 305, 379, 673, 1003
HphI GGTGA 2 cut(s) 440, 637
Hpy166II GTNNAC 5 cut(s) 139, 688, 772, 790, 913
Hpy188I TCNGA 8 cut(s) 121, 159, 186, 233, 267, 337, 384, 678
Hpy188III TCNNGA 8 cut(s) 170, 275, 302, 428, 515, 642, 901, 1013
Hpy8I GTNNAC 5 cut(s) 139, 688, 772, 790, 913
Hpy99I CGWCG 6 cut(s) 140, 143, 190, 214, 464, 902
HpyAV CCTTC 2 cut(s) 96, 863
HpyCH4III ACNGT 3 cut(s) 298, 955, 1019
HpyCH4IV ACGT 1 cut(s) 200
HpyCH4V TGCA 8 cut(s) 403, 412, 571, 800, 857, 878, 923, 977
HpyF3I CTNAG 3 cut(s) 39, 336, 783
HpySE526I ACGT 1 cut(s) 200
Hsp92I GRCGYC 1 cut(s) 894
Hsp92II CATG 2 cut(s) 607, 1039
HspAI GCGC 3 cut(s) 30, 124, 533
Kzo9I GATC 6 cut(s) 6, 159, 332, 340, 390, 539
LmnI GCTCC 7 cut(s) 107, 182, 196, 649, 700, 706, 964
Lsp1109I GCAGC 7 cut(s) 406, 619, 664, 818, 869, 935, 973
LweI GCATC 1 cut(s) 964
MaeI CTAG 3 cut(s) 93, 477, 705
MaeII ACGT 1 cut(s) 200
MaeIII GTNAC 5 cut(s) 196, 283, 425, 482, 625
MalI GATC 6 cut(s) 8, 161, 334, 342, 392, 541
MbiI CCGCTC 1 cut(s) 701
MboI GATC 6 cut(s) 6, 159, 332, 340, 390, 539
MboII GAAGA 3 cut(s) 52, 58, 933
MflI RGATCY 2 cut(s) 332, 390
MluCI AATT 7 cut(s) 148, 269, 572, 825, 852, 1008, 1057
MlyI GAGTC 3 cut(s) 51, 272, 667
MmeI TCCRAC 3 cut(s) 209, 310, 701
MspA1I CMGCKG 1 cut(s) 926
MspR9I CCNGG 4 cut(s) 49, 395, 692, 776
MvaI CCWGG 3 cut(s) 49, 395, 692
MvnI CGCG 1 cut(s) 30
NciI CCSGG 1 cut(s) 776
NcoI CCATGG 1 cut(s) 1035
NdeII GATC 6 cut(s) 6, 159, 332, 340, 390, 539
NheI GCTAGC 1 cut(s) 92
NlaIII CATG 2 cut(s) 607, 1039
NmuCI GTSAC 4 cut(s) 196, 283, 425, 625
PcsI WCGNNNNNNNCGW 3 cut(s) 105, 168, 206
PfeI GAWTC 4 cut(s) 234, 305, 379, 1003
PflFI GACNNNGTC 3 cut(s) 115, 281, 896
PflMI CCANNNNNTGG 1 cut(s) 955
PfoI TCCNGGA 1 cut(s) 393
PinAI ACCGGT 2 cut(s) 680, 880
PkrI GCNGC 7 cut(s) 421, 634, 654, 808, 859, 925, 988
PleI GAGTC 3 cut(s) 51, 272, 667
PpsI GAGTC 3 cut(s) 51, 272, 667
Psp6I CCWGG 3 cut(s) 47, 393, 690
PspGI CCWGG 3 cut(s) 47, 393, 690
PspPI GGNCC 2 cut(s) 644, 719
PstI CTGCAG 1 cut(s) 925
PsuI RGATCY 2 cut(s) 332, 390
PsyI GACNNNGTC 3 cut(s) 115, 281, 896
PvuII CAGCTG 1 cut(s) 926
RsaI GTAC 5 cut(s) 14, 689, 747, 773, 791
RsaNI GTAC 5 cut(s) 13, 688, 746, 772, 790
SalI GTCGAC 1 cut(s) 137
SatI GCNGC 7 cut(s) 420, 633, 653, 807, 858, 924, 987
Sau3AI GATC 6 cut(s) 6, 159, 332, 340, 390, 539
Sau96I GGNCC 2 cut(s) 644, 719
SchI GAGTC 3 cut(s) 51, 272, 667
ScrFI CCNGG 4 cut(s) 49, 395, 692, 776
SfaNI GCATC 1 cut(s) 964
SfcI CTRYAG 2 cut(s) 921, 969
SgrAI CRCCGGYG 1 cut(s) 488
SgrDI CGTCGACG 1 cut(s) 137
SinI GGWCC 2 cut(s) 644, 719
Sse9I AATT 7 cut(s) 148, 269, 572, 825, 852, 1008, 1057
SspMI CTAG 3 cut(s) 93, 477, 705
StyD4I CCNGG 4 cut(s) 47, 393, 690, 774
StyI CCWWGG 2 cut(s) 476, 1035
TaaI ACNGT 3 cut(s) 298, 955, 1019
TaiI ACGT 1 cut(s) 203
TaqI TCGA 5 cut(s) 9, 55, 138, 237, 671
TasI AATT 7 cut(s) 148, 269, 572, 825, 852, 1008, 1057
TatI WGTACW 1 cut(s) 789
TfiI GAWTC 4 cut(s) 234, 305, 379, 1003
TscAI CASTG 4 cut(s) 134, 616, 807, 925
TseFI GTSAC 4 cut(s) 196, 283, 425, 625
TseI GCWGC 7 cut(s) 419, 632, 652, 806, 857, 923, 986
Tsp45I GTSAC 4 cut(s) 196, 283, 425, 625
TspDTI ATGAA 2 cut(s) 620, 933
TspGWI ACGGA 1 cut(s) 142
TspRI CASTG 4 cut(s) 134, 616, 807, 925
Tth111I GACNNNGTC 3 cut(s) 115, 281, 896
Van91I CCANNNNNTGG 1 cut(s) 955
VpaK11BI GGWCC 2 cut(s) 644, 719
XapI RAATTY 4 cut(s) 148, 269, 825, 1008
XmaJI CCTAGG 1 cut(s) 476
XmiI GTMKAC 2 cut(s) 138, 912
XspI CTAG 3 cut(s) 93, 477, 705
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.