Rroxscaffold_3G00227470

PB1 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0002525
Physical Location & Seq
Reverse (-)
11599945 .. 11602920
2976 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00227470.1

Sequence Viewer

Length: 1194 bp
ATGGAGAATTACTCCAACCACTCCTACCCGGACTCCCGCGAGTCGTCCCCACGCTCCCGCGAAATCGACTGCGACACTCCCTCCTGGGACGATCAGCCTCCCTCCTCCGCCGCCGCCGCCGCCGCCGCCACCTACAAGGTCAAGCTCATGTGCAGCTACGGCGGCAAAATCCAGCCCCGACCCCACGACAACCAGCTCGCCTACGTCGGCGGCGACACCAAAATCCTCGCCGTCGATCGCAGCATCAAGCTCTCCGCCTTCATTTCCAAGCTCTCCTCCCTCTGCGAAACCCCCGACGGCGCCGTCTGCTTCAAGTACCAGCTCCCCGGCGAGGATCTCGACGCCCTCATCTCCGTCACCAACGACGAGGACCTCGACCACATGATGATCGAGTACGACCGCCTCTACCGCGCCTCCGCTAAGCCCGCGCGTCTCAGGCTCTTCCTCTTCCCCCTCAACCCTTCCGCTCCGGCCAGCTTCGGCTCCAACGAGTCCAAGTCCGAGCGCCAGTGGTTCGTCGACGCCTTGAATTCCGTTCAGATCCAGTCCCCCGAAGGCTCCTCTCCCACCGCTCCGGTCTCCGCCTCCTCCACCAACCCCGATTTCCTCTTCGGATTCGACAAGGGATACGCCGCCGCCGCCGCCGCTCCCGTCGCGAAATTGCCTGATTCCGTCACGCCTCCGACGGTTCCTGATTCGCTTGTGAAGAACACGCTCGCCGCCGGACCGGAATTGGTCTCGGAGCCGACGCTGTCGCAATTCGAGATTCAGAGGCAGATTCAGGAGCTACACAGAATGCAAATTGCTGCCGGTCACGAGCCGACTATCTATCAGAGAAAAAACGATGAGGTTCCGAACCCTCGGGGTTACACAGCTGATTATTACTCTCAGAAGCTTCCAGAAAACATAGCTCCGGCGCCACCGGGAGCCGCTCCGGTTCCAATGCCGGCACACATGCAATTTCCGACTTCTTATATGCCGGAAAGGCACATAAACACCGGAAGTTATCAGGTGGCGGCGGCTCCCGGTGGAGGCGAGCAGCCTGTGTACCTCATTTCCACGCCAGCTGGTACTGTGGCGCCAGTACCGGTTGCTGTTGATGCAAGACAAGGTGGTGGTGGTGGCGCTTTGAATCAAATTCAAGACGGTAAGGTGCTAGGTGAAGAGCAGGAGGGCTACAGTAAGAAACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

397

Amino Acids

42.68

Weight (kDa)

5.04

Isoelectric Point (pI)

49.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 62 - 151 3.9e-16 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 302
AccB1I GGYRCC 3 cut(s) 299, 916, 1078
AccBSI CCGCTC 4 cut(s) 467, 572, 647, 932
AccI GTMKAC 1 cut(s) 519
AccII CGCG 6 cut(s) 39, 60, 411, 428, 430, 656
AclWI GGATC 2 cut(s) 342, 535
AcoI YGGCCR 1 cut(s) 471
AcsI RAATTY 2 cut(s) 529, 1137
AcyI GRCGYC 5 cut(s) 300, 342, 522, 917, 1079
AfaI GTAC 5 cut(s) 317, 395, 1049, 1072, 1086
AfiI CCNNNNNNNGG 2 cut(s) 331, 1031
AgeI ACCGGT 1 cut(s) 1087
AgsI TTSAA 4 cut(s) 313, 529, 1132, 1142
AjnI CCWGG 1 cut(s) 83
AjuI GAANNNNNNNTTGG 2 cut(s) 587, 619
Alw26I GTCTC 3 cut(s) 437, 583, 742
AlwI GGATC 2 cut(s) 342, 535
Ama87I CYCGRG 1 cut(s) 861
AoxI GGCC 1 cut(s) 471
ApeKI GCWGC 4 cut(s) 153, 240, 806, 1039
ApoI RAATTY 2 cut(s) 529, 1137
AsiGI ACCGGT 1 cut(s) 1087
AspLEI GCGC 7 cut(s) 302, 413, 430, 507, 919, 1081, 1127
AspS9I GGNCC 2 cut(s) 370, 725
AsuC2I CCSGG 4 cut(s) 29, 327, 924, 1026
AsuHPI GGTGA 2 cut(s) 349, 1172
AvaI CYCGRG 1 cut(s) 861
AvaII GGWCC 2 cut(s) 370, 725
BanI GGYRCC 3 cut(s) 299, 916, 1078
BauI CACGAG 1 cut(s) 815
BbvI GCAGC 4 cut(s) 165, 252, 793, 1051
BceAI ACGGC 4 cut(s) 175, 215, 287, 313
BciT130I CCWGG 1 cut(s) 85
BciVI GTATCC 1 cut(s) 620
BcnI CCSGG 4 cut(s) 29, 327, 924, 1026
BcoDI GTCTC 3 cut(s) 437, 583, 742
BfaI CTAG 1 cut(s) 1157
BfmI CTRYAG 1 cut(s) 1177
BfoI RGCGCY 5 cut(s) 303, 508, 920, 1082, 1128
BfuI GTATCC 1 cut(s) 620
BglI GCCNNNNNGGC 1 cut(s) 985
BlpI GCTNAGC 1 cut(s) 420
Bme1390I CCNGG 5 cut(s) 29, 85, 327, 924, 1026
Bme18I GGWCC 2 cut(s) 370, 725
BmeT110I CYCGRG 1 cut(s) 861
BmgT120I GGNCC 2 cut(s) 370, 725
BmrFI CCNGG 5 cut(s) 29, 85, 327, 924, 1026
BmsI GCATC 2 cut(s) 252, 1090
BplI GAGNNNNNCTC 1 cut(s) 28
Bpu1102I GCTNAGC 1 cut(s) 420
BpuMI CCSGG 4 cut(s) 29, 327, 924, 1026
BsaHI GRCGYC 5 cut(s) 300, 342, 522, 917, 1079
BsaI GGTCTC 2 cut(s) 583, 742
BsaJI CCNNGG 3 cut(s) 84, 325, 860
BsaWI WCCGGW 5 cut(s) 574, 727, 934, 998, 1087
BsaXI ACNNNNNCTCC 6 cut(s) 17, 47, 65, 95, 398, 428
Bsc4I CCNNNNNNNGG 2 cut(s) 331, 1031
Bse118I RCCGGY 3 cut(s) 809, 946, 1087
Bse1I ACTGG 3 cut(s) 508, 544, 1082
BseBI CCWGG 1 cut(s) 85
BseDI CCNNGG 3 cut(s) 84, 325, 860
BseLI CCNNNNNNNGG 2 cut(s) 331, 1031
BseMII CTCAG 2 cut(s) 448, 902
BseNI ACTGG 3 cut(s) 508, 544, 1082
BseRI GAGGAG 4 cut(s) 94, 265, 550, 577
BseXI GCAGC 4 cut(s) 165, 252, 793, 1051
BsgI GTGCAG 1 cut(s) 172
Bsh1236I CGCG 6 cut(s) 39, 60, 411, 428, 430, 656
Bsh1285I CGRYCG 2 cut(s) 238, 400
BshFI GGCC 1 cut(s) 473
BshNI GGYRCC 3 cut(s) 299, 916, 1078
BshTI ACCGGT 1 cut(s) 1087
BsiEI CGRYCG 2 cut(s) 238, 400
BsiHKCI CYCGRG 1 cut(s) 861
BslFI GGGAC 3 cut(s) 31, 101, 532
BslI CCNNNNNNNGG 2 cut(s) 331, 1031
BsmAI GTCTC 3 cut(s) 437, 583, 742
BsmBI CGTCTC 1 cut(s) 437
BsmFI GGGAC 3 cut(s) 31, 101, 532
BsmI GAATGC 1 cut(s) 801
BsnI GGCC 1 cut(s) 473
Bso31I GGTCTC 2 cut(s) 583, 742
BsoBI CYCGRG 1 cut(s) 861
Bsp143I GATC 5 cut(s) 91, 235, 334, 387, 540
Bsp1720I GCTNAGC 1 cut(s) 420
Bsp68I TCGCGA 1 cut(s) 656
BspANI GGCC 1 cut(s) 473
BspCNI CTCAG 2 cut(s) 447, 901
BspFNI CGCG 6 cut(s) 39, 60, 411, 428, 430, 656
BspPI GGATC 2 cut(s) 342, 535
BspQI GCTCTTC 2 cut(s) 446, 1158
BspT107I GGYRCC 3 cut(s) 299, 916, 1078
BspTNI GGTCTC 2 cut(s) 583, 742
BsrBI CCGCTC 4 cut(s) 467, 572, 647, 932
BsrFI RCCGGY 3 cut(s) 809, 946, 1087
BsrI ACTGG 3 cut(s) 508, 544, 1082
BssAI RCCGGY 3 cut(s) 809, 946, 1087
BssECI CCNNGG 3 cut(s) 84, 325, 860
BssMI GATC 5 cut(s) 91, 235, 334, 387, 540
BssNI GRCGYC 5 cut(s) 300, 342, 522, 917, 1079
BssSI CACGAG 1 cut(s) 815
Bst2BI CACGAG 1 cut(s) 815
Bst2UI CCWGG 1 cut(s) 85
Bst4CI ACNGT 5 cut(s) 688, 1075, 1148, 1181, 1191
Bst6I CTCTTC 4 cut(s) 446, 452, 614, 1158
BstACI GRCGYC 5 cut(s) 300, 342, 522, 917, 1079
BstC8I GCNNGC 7 cut(s) 198, 426, 475, 717, 948, 1037, 1065
BstDEI CTNAG 3 cut(s) 420, 434, 888
BstFNI CGCG 6 cut(s) 39, 60, 411, 428, 430, 656
BstH2I RGCGCY 5 cut(s) 303, 508, 920, 1082, 1128
BstHHI GCGC 7 cut(s) 302, 413, 430, 507, 919, 1081, 1127
BstKTI GATC 5 cut(s) 94, 238, 337, 390, 543
BstMAI GTCTC 3 cut(s) 437, 583, 742
BstMBI GATC 5 cut(s) 91, 235, 334, 387, 540
BstMCI CGRYCG 2 cut(s) 238, 400
BstNI CCWGG 1 cut(s) 85
BstNSI RCATGY 1 cut(s) 958
BstSCI CCNGG 5 cut(s) 27, 83, 325, 922, 1024
BstSFI CTRYAG 1 cut(s) 1177
BstUI CGCG 6 cut(s) 39, 60, 411, 428, 430, 656
BstV1I GCAGC 4 cut(s) 165, 252, 793, 1051
BstX2I RGATCY 2 cut(s) 334, 540
BstYI RGATCY 2 cut(s) 334, 540
BsuI GTATCC 1 cut(s) 620
BsuRI GGCC 1 cut(s) 473
BtsIMutI CAGTG 1 cut(s) 515
BtuMI TCGCGA 1 cut(s) 656
Cac8I GCNNGC 7 cut(s) 198, 426, 475, 717, 948, 1037, 1065
CfoI GCGC 7 cut(s) 302, 413, 430, 507, 919, 1081, 1127
Cfr10I RCCGGY 3 cut(s) 809, 946, 1087
Cfr13I GGNCC 2 cut(s) 370, 725
CpoI CGGWCCG 1 cut(s) 725
CseI GACGC 4 cut(s) 350, 419, 530, 757
Csp6I GTAC 5 cut(s) 316, 394, 1048, 1071, 1085
CspAI ACCGGT 1 cut(s) 1087
CspI CGGWCCG 1 cut(s) 725
CviAII CATG 3 cut(s) 148, 382, 955
CviQI GTAC 5 cut(s) 316, 394, 1048, 1071, 1085
DdeI CTNAG 3 cut(s) 420, 434, 888
DinI GGCGCC 3 cut(s) 301, 918, 1080
DpnI GATC 5 cut(s) 93, 237, 336, 389, 542
DpnII GATC 5 cut(s) 91, 235, 334, 387, 540
DrdI GACNNNNNNGTC 1 cut(s) 302
DseDI GACNNNNNNGTC 1 cut(s) 302
EaeI YGGCCR 1 cut(s) 471
Eam1104I CTCTTC 4 cut(s) 446, 452, 614, 1158
EarI CTCTTC 4 cut(s) 446, 452, 614, 1158
EciI GGCGGA 3 cut(s) 97, 244, 571
Eco31I GGTCTC 2 cut(s) 583, 742
Eco47I GGWCC 2 cut(s) 370, 725
Eco88I CYCGRG 1 cut(s) 861
EcoO109I RGGNCCY 1 cut(s) 370
EcoRI GAATTC 1 cut(s) 529
EcoRII CCWGG 1 cut(s) 83
EgeI GGCGCC 3 cut(s) 301, 918, 1080
EheI GGCGCC 3 cut(s) 301, 918, 1080
Esp3I CGTCTC 1 cut(s) 437
FaeI CATG 3 cut(s) 151, 385, 958
FaiI YATR 7 cut(s) 149, 383, 908, 956, 975, 977, 992
FaqI GGGAC 3 cut(s) 31, 101, 532
FatI CATG 3 cut(s) 147, 381, 954
FauI CCCGC 3 cut(s) 44, 65, 433
FblI GTMKAC 1 cut(s) 519
FspBI CTAG 1 cut(s) 1157
GlaI GCGC 7 cut(s) 301, 412, 429, 506, 918, 1080, 1126
HaeII RGCGCY 5 cut(s) 303, 508, 920, 1082, 1128
HaeIII GGCC 1 cut(s) 473
HgaI GACGC 4 cut(s) 350, 419, 530, 757
HhaI GCGC 7 cut(s) 302, 413, 430, 507, 919, 1081, 1127
Hin1I GRCGYC 5 cut(s) 300, 342, 522, 917, 1079
Hin1II CATG 3 cut(s) 151, 385, 958
Hin6I GCGC 7 cut(s) 300, 411, 428, 505, 917, 1079, 1125
HinP1I GCGC 7 cut(s) 300, 411, 428, 505, 917, 1079, 1125
HincII GTYRAC 1 cut(s) 520
HindII GTYRAC 1 cut(s) 520
HindIII AAGCTT 1 cut(s) 893
HinfI GANTC 9 cut(s) 32, 41, 491, 615, 668, 695, 766, 778, 1132
HphI GGTGA 2 cut(s) 349, 1172
Hpy166II GTNNAC 2 cut(s) 520, 1048
Hpy188III TCNNGA 8 cut(s) 338, 655, 692, 763, 782, 815, 899, 1142
Hpy8I GTNNAC 2 cut(s) 520, 1048
HpyAV CCTTC 3 cut(s) 268, 471, 548
HpyCH4III ACNGT 5 cut(s) 688, 1075, 1148, 1181, 1191
HpyCH4IV ACGT 1 cut(s) 204
HpyCH4V TGCA 4 cut(s) 153, 799, 958, 1103
HpyF3I CTNAG 3 cut(s) 420, 434, 888
HpySE526I ACGT 1 cut(s) 204
Hsp92I GRCGYC 5 cut(s) 300, 342, 522, 917, 1079
Hsp92II CATG 3 cut(s) 151, 385, 958
HspAI GCGC 7 cut(s) 300, 411, 428, 505, 917, 1079, 1125
KasI GGCGCC 3 cut(s) 299, 916, 1078
KroI GCCGGC 1 cut(s) 946
KroNI GCCGGC 1 cut(s) 948
Kzo9I GATC 5 cut(s) 91, 235, 334, 387, 540
LguI GCTCTTC 2 cut(s) 446, 1158
Lsp1109I GCAGC 4 cut(s) 165, 252, 793, 1051
LweI GCATC 2 cut(s) 252, 1090
MaeI CTAG 1 cut(s) 1157
MaeII ACGT 1 cut(s) 204
MaeIII GTNAC 4 cut(s) 355, 673, 812, 866
MalI GATC 5 cut(s) 93, 237, 336, 389, 542
MbiI CCGCTC 4 cut(s) 467, 572, 647, 932
MboI GATC 5 cut(s) 91, 235, 334, 387, 540
MboII GAAGA 5 cut(s) 433, 439, 601, 718, 1175
MflI RGATCY 2 cut(s) 334, 540
MluCI AATT 8 cut(s) 7, 529, 659, 731, 758, 801, 959, 1137
Mly113I GGCGCC 3 cut(s) 300, 917, 1079
MlyI GAGTC 3 cut(s) 26, 50, 500
MmeI TCCRAC 4 cut(s) 39, 510, 707, 989
MroNI GCCGGC 1 cut(s) 946
MspA1I CMGCKG 2 cut(s) 875, 1067
MspR9I CCNGG 5 cut(s) 29, 85, 327, 924, 1026
Mva1269I GAATGC 1 cut(s) 801
MvaI CCWGG 1 cut(s) 85
MvnI CGCG 6 cut(s) 39, 60, 411, 428, 430, 656
NaeI GCCGGC 1 cut(s) 948
NarI GGCGCC 3 cut(s) 300, 917, 1079
NciI CCSGG 4 cut(s) 29, 327, 924, 1026
NdeII GATC 5 cut(s) 91, 235, 334, 387, 540
NgoMIV GCCGGC 1 cut(s) 946
NlaIII CATG 3 cut(s) 151, 385, 958
NmuCI GTSAC 3 cut(s) 355, 673, 812
NruI TCGCGA 1 cut(s) 656
NspI RCATGY 1 cut(s) 958
PciSI GCTCTTC 2 cut(s) 446, 1158
PcsI WCGNNNNNNNCGW 3 cut(s) 210, 372, 486
PctI GAATGC 1 cut(s) 801
PdiI GCCGGC 1 cut(s) 948
PfeI GAWTC 6 cut(s) 615, 668, 695, 766, 778, 1132
PflFI GACNNNGTC 1 cut(s) 751
PinAI ACCGGT 1 cut(s) 1087
Ple19I CGATCG 1 cut(s) 238
PleI GAGTC 3 cut(s) 26, 49, 499
PluTI GGCGCC 3 cut(s) 303, 920, 1082
PpsI GAGTC 3 cut(s) 26, 49, 499
PpuMI RGGWCCY 1 cut(s) 370
Psp5II RGGWCCY 1 cut(s) 370
Psp6I CCWGG 1 cut(s) 83
PspGI CCWGG 1 cut(s) 83
PspPI GGNCC 2 cut(s) 370, 725
PspPPI RGGWCCY 1 cut(s) 370
PsuI RGATCY 2 cut(s) 334, 540
PsyI GACNNNGTC 1 cut(s) 751
PvuI CGATCG 1 cut(s) 238
PvuII CAGCTG 2 cut(s) 875, 1067
RruI TCGCGA 1 cut(s) 656
RsaI GTAC 5 cut(s) 317, 395, 1049, 1072, 1086
RsaNI GTAC 5 cut(s) 316, 394, 1048, 1071, 1085
Rsr2I CGGWCCG 1 cut(s) 725
RsrII CGGWCCG 1 cut(s) 725
SalI GTCGAC 1 cut(s) 518
SapI GCTCTTC 2 cut(s) 446, 1158
Sau3AI GATC 5 cut(s) 91, 235, 334, 387, 540
Sau96I GGNCC 2 cut(s) 370, 725
SchI GAGTC 3 cut(s) 26, 50, 500
ScrFI CCNGG 5 cut(s) 29, 85, 327, 924, 1026
SfaNI GCATC 2 cut(s) 252, 1090
SfcI CTRYAG 1 cut(s) 1177
SfoI GGCGCC 3 cut(s) 301, 918, 1080
SgrDI CGTCGACG 1 cut(s) 518
SinI GGWCC 2 cut(s) 370, 725
Sse9I AATT 8 cut(s) 7, 529, 659, 731, 758, 801, 959, 1137
SspDI GGCGCC 3 cut(s) 299, 916, 1078
SspMI CTAG 1 cut(s) 1157
StyD4I CCNGG 5 cut(s) 27, 83, 325, 922, 1024
TaaI ACNGT 5 cut(s) 688, 1075, 1148, 1181, 1191
TaiI ACGT 1 cut(s) 207
TaqI TCGA 8 cut(s) 66, 234, 339, 375, 390, 519, 618, 762
TasI AATT 8 cut(s) 7, 529, 659, 731, 758, 801, 959, 1137
TfiI GAWTC 6 cut(s) 615, 668, 695, 766, 778, 1132
TscAI CASTG 1 cut(s) 515
TseFI GTSAC 3 cut(s) 355, 673, 812
TseI GCWGC 4 cut(s) 153, 240, 806, 1039
Tsp45I GTSAC 3 cut(s) 355, 673, 812
TspDTI ATGAA 1 cut(s) 250
TspGWI ACGGA 3 cut(s) 343, 523, 661
TspRI CASTG 1 cut(s) 515
Tth111I GACNNNGTC 1 cut(s) 751
VpaK11BI GGWCC 2 cut(s) 370, 725
XapI RAATTY 2 cut(s) 529, 1137
XceI RCATGY 1 cut(s) 958
XmiI GTMKAC 1 cut(s) 519
XspI CTAG 1 cut(s) 1157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.