pycom01g04160

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0005653
Physical Location & Seq
Forward (+)
3157820 .. 3163712
5893 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4206 bp
ATGGAAATAAATGAGCATGCGACTATAAGAATGAGTACTCAAAAGATCAATAGCTTATTGCATGTCAAAAGTACACAGTTTTTCTTTATGAAGTGCATTTGGACTGTTTTCGACATTTATGGAAACAAACGAGTTAGGACCATAAGAATGAGTACTCAAAAGATCAAAAGCTTGGATTTTGAATTTAATTCATCTGGGTTTTTATGTGGCGTCTCTATCTATATAAATGTGAAAATTTACATAATTGCAGTAATAGAAAGGAGCAGTAGAGTGGCTGCAGCCCACAGAAGCGCAAACAAGAGAAAAAAAAAATATTGGGAGAGCTTGGTTGCGAGTTTTAAAAGAGTTGGGATCTGGAGGCCTCAAAATTCAAACATCTGGCAACTAAGGGATATTGGGTTGAATACAAATAAAGCAGTGTTAGCTGAGTTGTTGAAATGTTTGCCGCAGCGACTCCACAATTACAGGAGGGTCGGTCATATTCAACAATATCTCAATTCATCTTGCTTTGCTTGCATTAGTAATTATTATACTATACTAATACTATTGTTACGCCTTTTTTTAAGTTCTGACCGTCATTCTGTATCTCAAAAAACTATTAGTAATTTAGTACGTCGTTTTTACATACATTTTATAGGGTATAGAAAAAATTTCACAGTTTTGGGTGAAACAACAAAAATGAGCTTATTCTCACAGTACAACATAAACAGCTGTTTTTCAAAGCACAGCAATACCAAACCCACTGTGTTTTGTTTGATTAAACCAATTTTTTCAAAGCACAACAATACCAAACCAGTTTTTTTTTTTTTTTTTTTTTCTCGTAAGAAGCTAAACATAGTGTGTAAAAATCATTTTCAATATTGGCTTTTAGTTAACAATTTTGCTTTCATTACCAAACGACGAAAATTACAAACTGAGAACAAAATAGGCTTTTGGTTATTTCCTTCTTTCTATTTTATTTTATTAATTTATAACTATAAAGAATTAGCCAAATTAATTAATTTAAATTTAGTGTTGATATTTTGTTTGCTATTTTGGAATTATTCAGTTGAATTGCTTTGCTTTGGAATGTGGATGCTTCATCCTGTCTGCATAGTTAACGAGAAAAACTCGGTCCGTGAATTGGGGACTTTGAGGTCTGGAGGGCCTCAAGTGACAGATATGCTGAGCTCTGATCAATCTTATGACGAAGAAAATGATGTGTTCTTCGACTCGGAGGACTTGTCACCTGCACGATCACTCGTAGTGAGTGAGGAGTACAAAATTTGGATGTATGAGCCACCAAGTGTTAAGGAGAGACGGGAGAGTTTTCTGCGAAAAATGGATTTAGTTGAGTTTGATTCTACAAAGTTTGCTTCTGTGGATATGGGATTGGAAAGAGTTTCGGAATGCAGTGGAGCTTCCCCAAGCTCTTGCATTTCGCCTTCTAGTCGTCATCAAGCAAACTTTATGTATAATACATTGGATAGTAACATACAAGGTAGCTCAAATTTGGCCTGGACTCACGATGCTGTTCATATTGCTTCACCTTCTGCTCGGAGAGAAGCTGAGGCTCCAGAAAAAGAATATCGGAATTCACAGGAGGGTGAAAAGAAGACGAAAGTTTGGTGGAAACAGTTTATAAACAAGAGGAAGAGAAGAGGAGGTAAAGTTGTGTCAAAGGTATCGAAACCAGATACTGAAATACCGAAACAAAATAGGATGAAGGTCAAGCAGAATGGTAAGAAGTGCATGGAGTTCACTGCACTATGCAGTGGGCAAGAAATTCATGCGCACAGGGGGTTCATTGGGGTGATGAAGTTTAGTCCCGATGGCCAGTATGTCGCAACTGGCGGTGAAGATGGGATTGTGTGCATCTGGCGTGTTACATTAGCAGATGCTTCTTGTACCTACTTAACACCTCAAGGGAACTTTGATAGCAAACTTAAGAAAATGAAGTCTGGTTATCGCGTGATCTTTCCTGAAAAGGGTTTTCGAATTCAGGAGTCACCGCTGCAAGAGTTTCATGGCCATTCCAGCGATGTATTAGACCTGGCTTGGTCGAGTTCAAATTATCTTCTTTCTTCGTCCATGGATAAAACTGTTCGTCTGTGGGATGTTAGTTTCGGTCACTGCCTAAATGTTTTCGATCACAATGACTATGTGACGTGCATTCAGTTCAATCCTGTCAACGACAATTATTTCATAAGTGGATCGATAGATGGGAAAGTTCGAGTTTGGGGACTCTCTGATAACCGAGTTGCTGACTGGGCTGATGTTCGAGATGTTATAACTGCTATATGTTATCAACCAGATGGAAAAGGTTTCGTGGTTGGTTCTGTTACAGGCACTTGCCACTTCTATGAAGTATCAGGGGACTATCTTCAGCTGGTTGTGCGAATGCGTATTCATGGTAGGAAGAAAACCTCCGGCAACCAAATCACTGGCATCAAGGAAAAGTCGCAGAGAGTTATGATAACATCAGAAGACTCGAAAGTCCGTATATTTGACGGAGTAGAACTTATAAGGAAATACAAAGGTGTGGATGCTTACCATATCTTGATTTGGGTAGATGATATGATACCCCGTTCTCTTGCAGCATATGCTCAAATTTGCATCTTACTAGTACATAGTTCGGGAAGTCAGACGTCAGCTTCTTTTACATCGAGCGGGAAACATATAATTTCAGTTGGAGAGGACTCGCGTGTTTATTTGTGGGACTATGACGAGCATTGTGTCTCATCATCATCCAAACAAACAAAATCTGTTCAATCCTGTGAGCATTTCTTATGCGAAGGTGTGTCTGTCGCGGTACCTTGGCCAGGCATGAGAAGAGAACAAAGGAGCTTGGAAACACAAGGGCATCTGGAGGCTGCTCCGGGAAGCAGAGATTCGCAATGTTTTTCTCTTGGAAATTGGTTTTTCTTAAATGGTCCGTGCAGGGGCGACTCTGCAACTTGGCCCGAAGAGAAACTTCCTCTGCCTTTATGGGATTTACCGGTCATACGAGATGAAAATGGTCAACATCACCGGCAACGTCAAGTTCATCATCGTCAGCAGCAGCAGCAAAATAAAGCACTCAATCGCACCGCTGAATCGGAACCATGGGGACTTGTCATTGTAACTGCTGGATGTGACGGAACAATCAGGACATTCCATAACTACGGATTGCCAGTCAGATTCAACACGCAACGCAACAAGAGTTCAGTCCCCACATTTCCATGTTTTTTCATGTCAGTCGAGCGTCCTTTTTCAATCCCTACATTTTTTTTTTTTTTTTTTTTTGAATATCAATTTAACGCCGACAACATAGGGAATTCGTACAATTACACAGTCCAAGGAATACTCATATTGTACAATTTCAATTATTGTTTTAGCCAATCATGTATAGAAAGGGCGCTTCAAAAGCATCCTTACCTACAGCTTTTGAGCTATGGATCGTCCTTGTATTTTGAGAACAATGCCAGATCACAGCTTACTCACCAATTCGTCTGTCCTGGTGAATCTTTTCTATCAAGCTCCCAAGAGCTGAGAAAGTGTCCTGACAGGGACGACAAATTGAGGTATGCAGCCGACTATATTACGACGCAGTTCTTCCCCATAAAAATCCTCATCGAAGTCGTGAAGCAGCCATGGCTCCTGCATTTCAAACTACATGTAAGAAAACAACTAAAGTGCTTGCAAAGGCAACCGATACTCATGACCATTTTGAAGACGCCACGCGTTGATAATCCAGCCCAACCAAATAAAATATACCCCAGAGGGATGTTCGGAAAGAAGAGATGCATATCCTTCTGGATAAATTTTGCTGGGGAAAAAGAATGTCATCTCAAACTAAGAACAGCAGTTATAACCCTTTTCATGAGTCAATTTCATATTATATATTTTACCCTATTTTTAGACTTTCGACTTCTTCTGGAGCAAAACATGGTCAAATGGACGAATTTTGGAGTAATTCCAATTAGAGGATACCAATGCTGGAATAATGACGTTTTGGGCTTAACTTGCGTTTTTGGAACCCAAGATGACCTCGAATGGGTTTCTGGCCTTCTGGAGATGTGTTCAGAACGTCCTGATCGTCAAAACAAGCTATCATGGGCAGGATTTGGAGGATATTTGAAGCTAAAACATGGCTGGACAAGTACTTGTTTATTATTATTTAGTTTCTTAGTGGAAATAAAATACTTGGCTGTTAGGGTTTGTGCGTTGGCCTTAGGGAGATATTAA

Protein Analysis

1402

Amino Acids

161.96

Weight (kDa)

9.28

Isoelectric Point (pI)

46.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_CAF1B_HIR1 PF24105 571 - 748 5.7e-17 CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3 PF25174 576 - 623 3.1e-06 THOC3 beta-propeller domain
WD40 PF00400 588 - 620 2.3e-06 WD domain, G-beta repeat
WD40_Gbeta PF25391 629 - 788 5.5e-10 G protein beta WD-40 repeat protein
WD40_CDC20-Fz PF24807 659 - 828 4.8e-12 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 661 - 741 2.9e-13 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 662 - 786 1.5e-14 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 663 - 835 4.5e-19 THOC3 beta-propeller domain
WD40 PF00400 663 - 699 4.5e-09 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 664 - 914 3.4e-27 WDR5 beta-propeller domain
WDR55 PF24796 665 - 830 5.3e-09 WDR55
Beta-prop_WDR36-Utp21_2nd PF25168 666 - 725 6.7e-09 WDR36/Utp21 second beta-propeller domain
EIF3I PF24805 666 - 832 1.3e-06 EIF3I
Beta-prop_WDR3_2nd PF25172 667 - 740 5e-09 WDR3 second beta-propeller domain
Beta-prop_Aladin PF25460 671 - 783 6.8e-07 Aladin seven-bladed propeller
Beta-prop_WDR90_POC16_2nd PF23393 686 - 818 1.3e-09 WDR90/POC16, second beta-propeller
Beta-prop_THOC3 PF25174 711 - 926 1.3e-12 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 722 - 834 8.7e-08 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 5 cut(s) 972, 1622, 2270, 2504, 3830
AarI CACCTGC 1 cut(s) 1237
AasI GACNNNNNNGTC 2 cut(s) 1135, 2474
AatII GACGTC 1 cut(s) 2630
Acc16I TGCGCA 1 cut(s) 1773
Acc36I ACCTGC 1 cut(s) 1237
Acc65I GGTACC 1 cut(s) 2791
AccB1I GGYRCC 1 cut(s) 2791
AccBSI CCGCTC 1 cut(s) 2649
AccII CGCG 4 cut(s) 1950, 2683, 2789, 3702
AciI CCGC 6 cut(s) 446, 1833, 1991, 2649, 2789, 3069
AclWI GGATC 3 cut(s) 359, 2200, 3424
AcoI YGGCCR 3 cut(s) 1813, 2008, 2798
AcuI CTGAAG 1 cut(s) 2348
AcyI GRCGYC 3 cut(s) 210, 2627, 3695
AdeI CACNNNGTG 1 cut(s) 1286
AfiI CCNNNNNNNGG 6 cut(s) 1123, 1967, 2801, 2921, 3944, 4015
AflII CTTAAG 1 cut(s) 1925
AflIII ACRYGT 2 cut(s) 3634, 3700
AgeI ACCGGT 1 cut(s) 2977
AhlI ACTAGT 1 cut(s) 2602
AjiI CACGTC 1 cut(s) 2148
AjnI CCWGG 4 cut(s) 1496, 2031, 2800, 3475
Alw21I GWGCWC 1 cut(s) 1172
Alw26I GTCTC 3 cut(s) 217, 1291, 2722
AlwI GGATC 3 cut(s) 359, 2200, 3424
AlwNI CAGNNNCTG 1 cut(s) 1679
AoxI GGCC 9 cut(s) 359, 1145, 1494, 1813, 2008, 2798, 2939, 4024, 4188
AseI ATTAAT 3 cut(s) 965, 995, 999
AsiGI ACCGGT 1 cut(s) 2977
Asp700I GAANNNNTTC 1 cut(s) 3486
Asp718I GGTACC 1 cut(s) 2791
AspLEI GCGC 3 cut(s) 293, 1774, 3379
AspS9I GGNCC 5 cut(s) 138, 1114, 1145, 2912, 2940
AsuC2I CCSGG 1 cut(s) 2859
AsuII TTCGAA 1 cut(s) 1975
AvaII GGWCC 3 cut(s) 138, 1114, 2912
AxyI CCTNAGG 1 cut(s) 4192
BalI TGGCCA 3 cut(s) 1815, 2010, 2800
BanI GGYRCC 1 cut(s) 2791
BanII GRGCYC 1 cut(s) 1172
BbsI GAAGAC 3 cut(s) 1601, 2472, 3698
Bbv12I GWGCWC 1 cut(s) 1172
BbvCI CCTCAGC 1 cut(s) 1548
BccI CCATC 4 cut(s) 1805, 1835, 2195, 2288
BciT130I CCWGG 4 cut(s) 1498, 2033, 2802, 3477
BciVI GTATCC 1 cut(s) 3941
BclI TGATCA 1 cut(s) 1174
BcnI CCSGG 1 cut(s) 2859
BcoDI GTCTC 3 cut(s) 217, 1291, 2722
BcuI ACTAGT 1 cut(s) 2602
BfaI CTAG 2 cut(s) 1428, 2603
BfmI CTRYAG 2 cut(s) 276, 3398
BfoI RGCGCY 1 cut(s) 3380
BfrI CTTAAG 1 cut(s) 1925
BfuAI ACCTGC 1 cut(s) 1237
BfuI GTATCC 1 cut(s) 3941
BlpI GCTNAGC 1 cut(s) 1166
BmcAI AGTACT 3 cut(s) 37, 154, 4123
Bme1390I CCNGG 5 cut(s) 1498, 2033, 2802, 2859, 3477
Bme18I GGWCC 3 cut(s) 138, 1114, 2912
BmgBI CACGTC 1 cut(s) 2148
BmgT120I GGNCC 5 cut(s) 138, 1114, 1145, 2912, 2940
BmiI GGNNCC 5 cut(s) 1554, 2793, 3081, 3617, 3997
BmrFI CCNGG 5 cut(s) 1498, 2033, 2802, 2859, 3477
BmrI ACTGGG 1 cut(s) 2257
BmuI ACTGGG 1 cut(s) 2257
BpiI GAAGAC 3 cut(s) 1601, 2472, 3698
BplI GAGNNNNNCTC 2 cut(s) 1094, 1126
BpmI CTGGAG 6 cut(s) 376, 1161, 1539, 2867, 3917, 4052
Bpu10I CCTNAGC 1 cut(s) 1548
Bpu1102I GCTNAGC 1 cut(s) 1166
Bpu14I TTCGAA 1 cut(s) 1975
BpuEI CTTGAG 2 cut(s) 1134, 1887
BpuMI CCSGG 1 cut(s) 2859
Bsa29I ATCGAT 1 cut(s) 2195
BsaBI GATNNNNATC 1 cut(s) 3766
BsaHI GRCGYC 3 cut(s) 210, 2627, 3695
BsaJI CCNNGG 5 cut(s) 2070, 2795, 3083, 3316, 3611
BsaWI WCCGGW 1 cut(s) 2977
BsaXI ACNNNNNCTCC 6 cut(s) 460, 490, 1208, 1238, 1635, 1665
Bsc4I CCNNNNNNNGG 6 cut(s) 1123, 1967, 2801, 2921, 3944, 4015
Bse118I RCCGGY 2 cut(s) 2977, 3009
Bse1I ACTGG 6 cut(s) 794, 1816, 1834, 2252, 2428, 3152
Bse21I CCTNAGG 1 cut(s) 4192
Bse3DI GCAATG 1 cut(s) 2882
Bse8I GATNNNNATC 1 cut(s) 3766
BseBI CCWGG 4 cut(s) 1498, 2033, 2802, 3477
BseCI ATCGAT 1 cut(s) 2195
BseDI CCNNGG 5 cut(s) 2070, 2795, 3083, 3316, 3611
BseJI GATNNNNATC 1 cut(s) 3766
BseLI CCNNNNNNNGG 6 cut(s) 1123, 1967, 2801, 2921, 3944, 4015
BseMI GCAATG 1 cut(s) 2882
BseMII CTCAG 5 cut(s) 417, 906, 1157, 1539, 3500
BseNI ACTGG 6 cut(s) 794, 1816, 1834, 2252, 2428, 3152
BseRI GAGGAG 2 cut(s) 1268, 1656
BseYI CCCAGC 1 cut(s) 3788
BsgI GTGCAG 3 cut(s) 1215, 1728, 2938
Bsh1236I CGCG 4 cut(s) 1950, 2683, 2789, 3702
BshFI GGCC 9 cut(s) 361, 1147, 1496, 1815, 2010, 2800, 2941, 4026, 4190
BshNI GGYRCC 1 cut(s) 2791
BshTI ACCGGT 1 cut(s) 2977
BshVI ATCGAT 1 cut(s) 2195
BsiHKAI GWGCWC 1 cut(s) 1172
BsiSI CCGG 4 cut(s) 2409, 2858, 2978, 3010
BslFI GGGAC 8 cut(s) 1141, 1791, 2235, 2369, 2711, 3102, 3173, 3542
BslI CCNNNNNNNGG 6 cut(s) 1123, 1967, 2801, 2921, 3944, 4015
BsmAI GTCTC 3 cut(s) 217, 1291, 2722
BsmBI CGTCTC 2 cut(s) 217, 1291
BsmFI GGGAC 8 cut(s) 1141, 1791, 2235, 2369, 2711, 3102, 3173, 3542
BsmI GAATGC 3 cut(s) 1394, 2151, 2385
BsnI GGCC 9 cut(s) 361, 1147, 1496, 1815, 2010, 2800, 2941, 4026, 4190
Bsp119I TTCGAA 1 cut(s) 1975
Bsp1286I GDGCHC 1 cut(s) 1172
Bsp1407I TGTACA 1 cut(s) 3333
Bsp1720I GCTNAGC 1 cut(s) 1166
Bsp19I CCATGG 3 cut(s) 2070, 3083, 3611
BspACI CCGC 6 cut(s) 446, 1833, 1991, 2649, 2789, 3069
BspANI GGCC 9 cut(s) 361, 1147, 1496, 1815, 2010, 2800, 2941, 4026, 4190
BspCNI CTCAG 5 cut(s) 418, 907, 1158, 1540, 3501
BspDI ATCGAT 1 cut(s) 2195
BspFNI CGCG 4 cut(s) 1950, 2683, 2789, 3702
BspHI TCATGA 2 cut(s) 3678, 3840
BspLI GGNNCC 5 cut(s) 1554, 2793, 3081, 3617, 3997
BspMAI CTGCAG 1 cut(s) 280
BspMI ACCTGC 1 cut(s) 1237
BspPI GGATC 3 cut(s) 359, 2200, 3424
BspT104I TTCGAA 1 cut(s) 1975
BspT107I GGYRCC 1 cut(s) 2791
BspTI CTTAAG 1 cut(s) 1925
BsrBI CCGCTC 1 cut(s) 2649
BsrDI GCAATG 1 cut(s) 2882
BsrFI RCCGGY 2 cut(s) 2977, 3009
BsrGI TGTACA 1 cut(s) 3333
BsrI ACTGG 6 cut(s) 794, 1816, 1834, 2252, 2428, 3152
BssAI RCCGGY 2 cut(s) 2977, 3009
BssECI CCNNGG 5 cut(s) 2070, 2795, 3083, 3316, 3611
BssNI GRCGYC 3 cut(s) 210, 2627, 3695
BssT1I CCWWGG 5 cut(s) 2070, 2795, 3083, 3316, 3611
Bst2UI CCWGG 4 cut(s) 1498, 2033, 2802, 3477
Bst4CI ACNGT 9 cut(s) 78, 106, 575, 658, 696, 745, 1617, 2083, 3313
Bst6I CTCTTC 5 cut(s) 1628, 1633, 2806, 2940, 3752
BstACI GRCGYC 3 cut(s) 210, 2627, 3695
BstAFI CTTAAG 1 cut(s) 1925
BstAPI GCANNNNNTGC 1 cut(s) 2582
BstAUI TGTACA 1 cut(s) 3333
BstBI TTCGAA 1 cut(s) 1975
BstC8I GCNNGC 3 cut(s) 18, 514, 3659
BstDEI CTNAG 9 cut(s) 386, 426, 915, 1166, 1548, 3509, 3815, 4147, 4192
BstDSI CCRYGG 3 cut(s) 2070, 3083, 3611
BstENI CCTNNNNNAGG 1 cut(s) 2799
BstFNI CGCG 4 cut(s) 1950, 2683, 2789, 3702
BstH2I RGCGCY 1 cut(s) 3380
BstHHI GCGC 3 cut(s) 293, 1774, 3379
BstMAI GTCTC 3 cut(s) 217, 1291, 2722
BstNI CCWGG 4 cut(s) 1498, 2033, 2802, 3477
BstNSI RCATGY 3 cut(s) 20, 65, 3638
BstSCI CCNGG 5 cut(s) 1496, 2031, 2800, 2857, 3475
BstSFI CTRYAG 2 cut(s) 276, 3398
BstUI CGCG 4 cut(s) 1950, 2683, 2789, 3702
BstV2I GAAGAC 3 cut(s) 1601, 2472, 3698
BstX2I RGATCY 1 cut(s) 351
BstXI CCANNNNNNTGG 1 cut(s) 2423
BstYI RGATCY 1 cut(s) 351
Bsu15I ATCGAT 1 cut(s) 2195
Bsu36I CCTNAGG 1 cut(s) 4192
BsuI GTATCC 1 cut(s) 3941
BsuRI GGCC 9 cut(s) 361, 1147, 1496, 1815, 2010, 2800, 2941, 4026, 4190
BsuTUI ATCGAT 1 cut(s) 2195
BtgI CCRYGG 3 cut(s) 2070, 3083, 3611
BtgZI GCGATG 1 cut(s) 2034
BtrI CACGTC 1 cut(s) 2148
BtsI GCAGTG 5 cut(s) 423, 1399, 1740, 1759, 2110
BtsIMutI CAGTG 7 cut(s) 423, 741, 1399, 1740, 1759, 2110, 2421
BveI ACCTGC 1 cut(s) 1237
Cac8I GCNNGC 3 cut(s) 18, 514, 3659
CaiI CAGNNNCTG 1 cut(s) 1679
CciI TCATGA 2 cut(s) 3678, 3840
CfoI GCGC 3 cut(s) 293, 1774, 3379
Cfr10I RCCGGY 2 cut(s) 2977, 3009
Cfr13I GGNCC 5 cut(s) 138, 1114, 1145, 2912, 2940
ClaI ATCGAT 1 cut(s) 2195
CpoI CGGWCCG 1 cut(s) 1114
CseI GACGC 4 cut(s) 199, 3212, 3574, 3703
CspAI ACCGGT 1 cut(s) 2977
CspI CGGWCCG 1 cut(s) 1114
DdeI CTNAG 9 cut(s) 386, 426, 915, 1166, 1548, 3509, 3815, 4147, 4192
DraI TTTAAA 2 cut(s) 340, 1005
DraIII CACNNNGTG 1 cut(s) 1286
DrdI GACNNNNNNGTC 2 cut(s) 1135, 2474
DseDI GACNNNNNNGTC 2 cut(s) 1135, 2474
EaeI YGGCCR 3 cut(s) 1813, 2008, 2798
Eam1104I CTCTTC 5 cut(s) 1628, 1633, 2806, 2940, 3752
EarI CTCTTC 5 cut(s) 1628, 1633, 2806, 2940, 3752
Ecl136II GAGCTC 1 cut(s) 1170
Eco130I CCWWGG 5 cut(s) 2070, 2795, 3083, 3316, 3611
Eco147I AGGCCT 1 cut(s) 361
Eco24I GRGCYC 1 cut(s) 1172
Eco47I GGWCC 3 cut(s) 138, 1114, 2912
Eco53kI GAGCTC 1 cut(s) 1170
Eco57I CTGAAG 1 cut(s) 2348
Eco81I CCTNAGG 1 cut(s) 4192
EcoICRI GAGCTC 1 cut(s) 1170
EcoNI CCTNNNNNAGG 1 cut(s) 2799
EcoO109I RGGNCCY 1 cut(s) 1145
EcoRI GAATTC 3 cut(s) 1573, 1977, 3295
EcoRII CCWGG 4 cut(s) 1496, 2031, 2800, 3475
EcoT14I CCWWGG 5 cut(s) 2070, 2795, 3083, 3316, 3611
EcoT22I ATGCAT 1 cut(s) 3767
EcoT38I GRGCYC 1 cut(s) 1172
ErhI CCWWGG 5 cut(s) 2070, 2795, 3083, 3316, 3611
Esp3I CGTCTC 2 cut(s) 217, 1291
FalI AAGNNNNNCTT 2 cut(s) 2937, 2969
FaqI GGGAC 8 cut(s) 1141, 1791, 2235, 2369, 2711, 3102, 3173, 3542
FauI CCCGC 1 cut(s) 2642
FauNDI CATATG 1 cut(s) 2581
FbaI TGATCA 1 cut(s) 1174
FriOI GRGCYC 1 cut(s) 1172
FspAI RTGCGCAY 1 cut(s) 1773
FspBI CTAG 2 cut(s) 1428, 2603
FspI TGCGCA 1 cut(s) 1773
GlaI GCGC 3 cut(s) 292, 1773, 3378
GsaI CCCAGC 1 cut(s) 3792
GsuI CTGGAG 6 cut(s) 376, 1161, 1539, 2867, 3917, 4052
HaeII RGCGCY 1 cut(s) 3380
HaeIII GGCC 9 cut(s) 361, 1147, 1496, 1815, 2010, 2800, 2941, 4026, 4190
HapII CCGG 4 cut(s) 2409, 2858, 2978, 3010
HgaI GACGC 4 cut(s) 199, 3212, 3574, 3703
HhaI GCGC 3 cut(s) 293, 1774, 3379
Hin1I GRCGYC 3 cut(s) 210, 2627, 3695
Hin6I GCGC 3 cut(s) 291, 1772, 3377
HinP1I GCGC 3 cut(s) 291, 1772, 3377
HincII GTYRAC 4 cut(s) 874, 1099, 2170, 3002
HindII GTYRAC 4 cut(s) 874, 1099, 2170, 3002
HindIII AAGCTT 1 cut(s) 169
HpaI GTTAAC 2 cut(s) 874, 1099
HpaII CCGG 4 cut(s) 2409, 2858, 2978, 3010
Hpy166II GTNNAC 6 cut(s) 74, 874, 1099, 1740, 2170, 3002
Hpy8I GTNNAC 6 cut(s) 74, 874, 1099, 1740, 2170, 3002
Hpy99I CGWCG 3 cut(s) 618, 903, 3568
HpyAV CCTTC 7 cut(s) 954, 1434, 1539, 1699, 2768, 3781, 4037
HpyCH4III ACNGT 9 cut(s) 78, 106, 575, 658, 696, 745, 1617, 2083, 3313
HpyCH4IV ACGT 6 cut(s) 613, 2147, 2627, 3016, 3969, 4048
HpyF3I CTNAG 9 cut(s) 386, 426, 915, 1166, 1548, 3509, 3815, 4147, 4192
HpySE526I ACGT 6 cut(s) 613, 2147, 2627, 3016, 3969, 4048
Hsp92I GRCGYC 3 cut(s) 210, 2627, 3695
HspAI GCGC 3 cut(s) 291, 1772, 3377
KpnI GGTACC 1 cut(s) 2795
Ksp22I TGATCA 1 cut(s) 1174
KspAI GTTAAC 2 cut(s) 874, 1099
LmnI GCTCC 8 cut(s) 261, 1397, 1558, 2823, 2860, 3503, 3621, 3898
MaeI CTAG 2 cut(s) 1428, 2603
MaeII ACGT 6 cut(s) 613, 2147, 2627, 3016, 3969, 4048
MbiI CCGCTC 1 cut(s) 2649
MflI RGATCY 1 cut(s) 351
MhlI GDGCHC 1 cut(s) 1172
MlsI TGGCCA 3 cut(s) 1815, 2010, 2800
MluI ACGCGT 1 cut(s) 3700
MluNI TGGCCA 3 cut(s) 1815, 2010, 2800
MlyI GAGTC 9 cut(s) 447, 1205, 1495, 1994, 2217, 2462, 2672, 2921, 3853
MmeI TCCRAC 1 cut(s) 2650
Mox20I TGGCCA 3 cut(s) 1815, 2010, 2800
Mph1103I ATGCAT 1 cut(s) 3767
MroXI GAANNNNTTC 1 cut(s) 3486
MscI TGGCCA 3 cut(s) 1815, 2010, 2800
MslI CAYNNNNRTG 4 cut(s) 146, 1790, 2340, 3199
Msp20I TGGCCA 3 cut(s) 1815, 2010, 2800
MspA1I CMGCKG 4 cut(s) 711, 1993, 2368, 3071
MspCI CTTAAG 1 cut(s) 1925
MspI CCGG 4 cut(s) 2409, 2858, 2978, 3010
MspR9I CCNGG 5 cut(s) 1498, 2033, 2802, 2859, 3477
Mva1269I GAATGC 3 cut(s) 1394, 2151, 2385
MvaI CCWGG 4 cut(s) 1498, 2033, 2802, 3477
MvnI CGCG 4 cut(s) 1950, 2683, 2789, 3702
NciI CCSGG 1 cut(s) 2859
NcoI CCATGG 3 cut(s) 2070, 3083, 3611
NdeI CATATG 1 cut(s) 2581
NlaIV GGNNCC 5 cut(s) 1554, 2793, 3081, 3617, 3997
NmuCI GTSAC 6 cut(s) 1153, 1224, 1986, 2108, 2143, 3113
NsbI TGCGCA 1 cut(s) 1773
NsiI ATGCAT 1 cut(s) 3767
NspI RCATGY 3 cut(s) 20, 65, 3638
NspV TTCGAA 1 cut(s) 1975
PacI TTAATTAA 1 cut(s) 999
PaeI GCATGC 1 cut(s) 20
PagI TCATGA 2 cut(s) 3678, 3840
PaqCI CACCTGC 1 cut(s) 1237
PceI AGGCCT 1 cut(s) 361
PciI ACATGT 1 cut(s) 3634
PcsI WCGNNNNNNNCGW 1 cut(s) 4054
PctI GAATGC 3 cut(s) 1394, 2151, 2385
PdmI GAANNNNTTC 1 cut(s) 3486
PfeI GAWTC 5 cut(s) 1340, 2870, 3074, 3159, 3482
PfoI TCCNGGA 1 cut(s) 2857
PinAI ACCGGT 1 cut(s) 2977
PleI GAGTC 9 cut(s) 447, 1205, 1495, 1993, 2217, 2462, 2672, 2921, 3852
PpsI GAGTC 9 cut(s) 447, 1205, 1495, 1993, 2217, 2462, 2672, 2921, 3852
PscI ACATGT 1 cut(s) 3634
PshBI ATTAAT 3 cut(s) 965, 995, 999
PsiI TTATAA 5 cut(s) 972, 1622, 2270, 2504, 3830
Psp124BI GAGCTC 1 cut(s) 1172
Psp6I CCWGG 4 cut(s) 1496, 2031, 2800, 3475
PspFI CCCAGC 1 cut(s) 3788
PspGI CCWGG 4 cut(s) 1496, 2031, 2800, 3475
PspN4I GGNNCC 5 cut(s) 1554, 2793, 3081, 3617, 3997
PspPI GGNCC 5 cut(s) 138, 1114, 1145, 2912, 2940
PstI CTGCAG 1 cut(s) 280
PstNI CAGNNNCTG 1 cut(s) 1679
PsuI RGATCY 1 cut(s) 351
PvuII CAGCTG 2 cut(s) 711, 2368
RseI CAYNNNNRTG 4 cut(s) 146, 1790, 2340, 3199
Rsr2I CGGWCCG 1 cut(s) 1114
RsrII CGGWCCG 1 cut(s) 1114
SacI GAGCTC 1 cut(s) 1172
Sau96I GGNCC 5 cut(s) 138, 1114, 1145, 2912, 2940
ScaI AGTACT 3 cut(s) 37, 154, 4123
SchI GAGTC 9 cut(s) 447, 1205, 1495, 1994, 2217, 2462, 2672, 2921, 3853
ScrFI CCNGG 5 cut(s) 1498, 2033, 2802, 2859, 3477
SduI GDGCHC 1 cut(s) 1172
SfcI CTRYAG 2 cut(s) 276, 3398
SfuI TTCGAA 1 cut(s) 1975
SinI GGWCC 3 cut(s) 138, 1114, 2912
SmiI ATTTAAAT 1 cut(s) 1005
SmiMI CAYNNNNRTG 4 cut(s) 146, 1790, 2340, 3199
SmlI CTYRAG 3 cut(s) 1149, 1902, 1925
SmoI CTYRAG 3 cut(s) 1149, 1902, 1925
SpeI ACTAGT 1 cut(s) 2602
SphI GCATGC 1 cut(s) 20
SseBI AGGCCT 1 cut(s) 361
SsiI CCGC 6 cut(s) 446, 1833, 1991, 2649, 2789, 3069
SspI AATATT 2 cut(s) 314, 860
SspMI CTAG 2 cut(s) 1428, 2603
SstI GAGCTC 1 cut(s) 1172
StuI AGGCCT 1 cut(s) 361
StyD4I CCNGG 5 cut(s) 1496, 2031, 2800, 2857, 3475
StyI CCWWGG 5 cut(s) 2070, 2795, 3083, 3316, 3611
SwaI ATTTAAAT 1 cut(s) 1005
TaaI ACNGT 9 cut(s) 78, 106, 575, 658, 696, 745, 1617, 2083, 3313
TaiI ACGT 6 cut(s) 616, 2150, 2630, 3019, 3972, 4051
TaqII GACCGA 3 cut(s) 464, 1102, 2096
TatI WGTACW 8 cut(s) 35, 71, 152, 696, 1257, 2605, 3333, 4121
TauI GCSGC 1 cut(s) 448
TfiI GAWTC 5 cut(s) 1340, 2870, 3074, 3159, 3482
TscAI CASTG 7 cut(s) 423, 748, 1399, 1747, 1759, 2117, 2428
TseFI GTSAC 6 cut(s) 1153, 1224, 1986, 2108, 2143, 3113
Tsp45I GTSAC 6 cut(s) 1153, 1224, 1986, 2108, 2143, 3113
TspGWI ACGGA 6 cut(s) 1106, 2468, 2505, 2904, 3132, 3159
TspRI CASTG 7 cut(s) 423, 748, 1399, 1747, 1759, 2117, 2428
Vha464I CTTAAG 1 cut(s) 1925
VpaK11BI GGWCC 3 cut(s) 138, 1114, 2912
VspI ATTAAT 3 cut(s) 965, 995, 999
XagI CCTNNNNNAGG 1 cut(s) 2799
XceI RCATGY 3 cut(s) 20, 65, 3638
XmnI GAANNNNTTC 1 cut(s) 3486
XspI CTAG 2 cut(s) 1428, 2603
ZraI GACGTC 1 cut(s) 2628
ZrmI AGTACT 3 cut(s) 37, 154, 4123
Zsp2I ATGCAT 1 cut(s) 3767
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.