pycom01g10830

Protein SDE2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0009131
Physical Location & Seq
Forward (+)
11611015 .. 11611955
941 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 528 bp
ATGCCCTGGTCAAGGATCGATCTTGATATTGAGAAGAATGAGATGGTTGGACCAGTGATTTCCGAAGAGAAGATAGTTTGGAGTGCAAGTGTTACTTGTTCAGATCCTGATGTGGTTTCGGGAGCTGAAGCAGAGCAAGATAGAAAGATAGGTTGCAATGGACCTGAGATGGGAAATTTGGAGAAAGTAGTTGGTCAATCTGAGAAGGTTTCTAGTTCAGGGCATGGGCAAGAAATTGAAAGTGCATCACTTGTTGCTGAAGCCAATGGTTCTTCAGAGTCCAATCCAGAAGTTCGGGAGGAAACAGTTGCGAGTGGAGACACTGCAGTGATGGAAAAGCCACTGAATTTTGATGAGTATAATTCAGCAGCAGAAATGGAGGTTCTTGGCCGGGAAAGGTTAAAGTCTGAACTGCAAACTCGTGGGTTAAAATGTGGGGGTACTCTACAGGAGCAAGCTGCGAGGCTTTTCATGCTAAAAACTACCCCAATCGAGAAGATTCCAAAGAAGCTCCTTGCAAAGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Telomere_Sde2 Telomere_Sde2_2

Protein Analysis

176

Amino Acids

18.98

Weight (kDa)

4.76

Isoelectric Point (pI)

37.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SDE2_2C PF13297 96 - 174 1.2e-27 Replication stress response SDE2 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 23, 98
AcoI YGGCCR 1 cut(s) 388
AcsI RAATTY 2 cut(s) 175, 346
AcuI CTGAAG 3 cut(s) 147, 258, 279
AfaI GTAC 1 cut(s) 442
AfiI CCNNNNNNNGG 2 cut(s) 12, 170
AgsI TTSAA 1 cut(s) 239
AjnI CCWGG 1 cut(s) 5
AleI CACNNNNGTG 1 cut(s) 326
AluBI AGCT 3 cut(s) 125, 458, 511
AluI AGCT 3 cut(s) 125, 458, 511
Alw26I GTCTC 1 cut(s) 312
AlwI GGATC 2 cut(s) 23, 98
AlwNI CAGNNNCTG 1 cut(s) 107
AoxI GGCC 1 cut(s) 388
ApeKI GCWGC 2 cut(s) 368, 458
ApoI RAATTY 2 cut(s) 175, 346
AspS9I GGNCC 2 cut(s) 50, 161
AsuC2I CCSGG 1 cut(s) 392
AvaII GGWCC 2 cut(s) 50, 161
BauI CACGAG 1 cut(s) 420
BbvI GCAGC 2 cut(s) 380, 445
BccI CCATC 3 cut(s) 37, 163, 325
BciT130I CCWGG 1 cut(s) 7
BcnI CCSGG 1 cut(s) 392
BcoDI GTCTC 1 cut(s) 312
BfaI CTAG 1 cut(s) 213
BfmI CTRYAG 2 cut(s) 324, 446
BisI GCNGC 2 cut(s) 369, 459
BlsI GCNGC 2 cut(s) 370, 460
Bme1390I CCNGG 2 cut(s) 7, 392
Bme18I GGWCC 2 cut(s) 50, 161
BmgT120I GGNCC 2 cut(s) 50, 161
BmrFI CCNGG 2 cut(s) 7, 392
BmsI GCATC 1 cut(s) 254
BpuMI CCSGG 1 cut(s) 392
Bsa29I ATCGAT 1 cut(s) 18
BsaJI CCNNGG 1 cut(s) 5
BsaXI ACNNNNNCTCC 2 cut(s) 73, 103
Bsc4I CCNNNNNNNGG 2 cut(s) 12, 170
Bse1I ACTGG 1 cut(s) 53
Bse3DI GCAATG 1 cut(s) 163
BseBI CCWGG 1 cut(s) 7
BseCI ATCGAT 1 cut(s) 18
BseDI CCNNGG 1 cut(s) 5
BseLI CCNNNNNNNGG 2 cut(s) 12, 170
BseMI GCAATG 1 cut(s) 163
BseMII CTCAG 2 cut(s) 156, 192
BseNI ACTGG 1 cut(s) 53
BseXI GCAGC 2 cut(s) 380, 445
BshFI GGCC 1 cut(s) 390
BshVI ATCGAT 1 cut(s) 18
BsiSI CCGG 1 cut(s) 391
BslI CCNNNNNNNGG 2 cut(s) 12, 170
BsmAI GTCTC 1 cut(s) 312
BsnI GGCC 1 cut(s) 390
Bsp143I GATC 3 cut(s) 15, 19, 103
BspANI GGCC 1 cut(s) 390
BspCNI CTCAG 2 cut(s) 157, 193
BspDI ATCGAT 1 cut(s) 18
BspMAI CTGCAG 1 cut(s) 328
BspPI GGATC 2 cut(s) 23, 98
BsrDI GCAATG 1 cut(s) 163
BsrI ACTGG 1 cut(s) 53
BssECI CCNNGG 1 cut(s) 5
BssMI GATC 3 cut(s) 15, 19, 103
BssSI CACGAG 1 cut(s) 420
Bst2BI CACGAG 1 cut(s) 420
Bst2UI CCWGG 1 cut(s) 7
Bst4CI ACNGT 1 cut(s) 307
Bst6I CTCTTC 1 cut(s) 60
BstC8I GCNNGC 1 cut(s) 456
BstDEI CTNAG 2 cut(s) 165, 201
BstENI CCTNNNNNAGG 1 cut(s) 10
BstKTI GATC 3 cut(s) 18, 22, 106
BstMAI GTCTC 1 cut(s) 312
BstMBI GATC 3 cut(s) 15, 19, 103
BstMWI GCNNNNNNNGC 1 cut(s) 472
BstNI CCWGG 1 cut(s) 7
BstSCI CCNGG 2 cut(s) 5, 390
BstSFI CTRYAG 2 cut(s) 324, 446
BstV1I GCAGC 2 cut(s) 380, 445
BstX2I RGATCY 1 cut(s) 103
BstYI RGATCY 1 cut(s) 103
Bsu15I ATCGAT 1 cut(s) 18
BsuRI GGCC 1 cut(s) 390
BsuTUI ATCGAT 1 cut(s) 18
BtsI GCAGTG 2 cut(s) 321, 333
BtsIMutI CAGTG 4 cut(s) 60, 321, 333, 341
Cac8I GCNNGC 1 cut(s) 456
CaiI CAGNNNCTG 1 cut(s) 107
Cfr13I GGNCC 2 cut(s) 50, 161
ClaI ATCGAT 1 cut(s) 18
Csp6I GTAC 1 cut(s) 441
CviAII CATG 2 cut(s) 224, 472
CviJI RGCY 7 cut(s) 125, 263, 340, 390, 458, 466, 511
CviKI_1 RGCY 7 cut(s) 125, 263, 340, 390, 458, 466, 511
CviQI GTAC 1 cut(s) 441
DdeI CTNAG 2 cut(s) 165, 201
DpnI GATC 3 cut(s) 17, 21, 105
DpnII GATC 3 cut(s) 15, 19, 103
EaeI YGGCCR 1 cut(s) 388
Eam1104I CTCTTC 1 cut(s) 60
EarI CTCTTC 1 cut(s) 60
Eco47I GGWCC 2 cut(s) 50, 161
Eco57I CTGAAG 3 cut(s) 147, 258, 279
EcoNI CCTNNNNNAGG 1 cut(s) 10
EcoRII CCWGG 1 cut(s) 5
FaeI CATG 2 cut(s) 227, 475
FaiI YATR 3 cut(s) 225, 360, 473
FalI AAGNNNNNCTT 2 cut(s) 79, 111
FatI CATG 2 cut(s) 223, 471
Fnu4HI GCNGC 2 cut(s) 369, 459
Fsp4HI GCNGC 2 cut(s) 369, 459
FspBI CTAG 1 cut(s) 213
GluI GCNGC 2 cut(s) 369, 459
HaeIII GGCC 1 cut(s) 390
HapII CCGG 1 cut(s) 391
Hin1II CATG 2 cut(s) 227, 475
HinfI GANTC 2 cut(s) 278, 499
HpaII CCGG 1 cut(s) 391
Hpy188I TCNGA 5 cut(s) 64, 103, 202, 277, 409
Hpy188III TCNNGA 6 cut(s) 23, 107, 120, 287, 296, 493
HpyAV CCTTC 1 cut(s) 199
HpyCH4III ACNGT 1 cut(s) 307
HpyCH4V TGCA 6 cut(s) 86, 156, 245, 326, 415, 518
HpyF10VI GCNNNNNNNGC 1 cut(s) 472
HpyF3I CTNAG 2 cut(s) 165, 201
Hsp92II CATG 2 cut(s) 227, 475
Kzo9I GATC 3 cut(s) 15, 19, 103
LmnI GCTCC 3 cut(s) 122, 451, 516
LpnPI CCDG 8 cut(s) 19, 66, 120, 177, 204, 300, 404, 434
Lsp1109I GCAGC 2 cut(s) 380, 445
LweI GCATC 1 cut(s) 254
MaeI CTAG 1 cut(s) 213
MaeIII GTNAC 1 cut(s) 91
MalI GATC 3 cut(s) 17, 21, 105
MboI GATC 3 cut(s) 15, 19, 103
MboII GAAGA 5 cut(s) 46, 77, 82, 264, 508
MflI RGATCY 1 cut(s) 103
MluCI AATT 4 cut(s) 175, 234, 346, 361
MlyI GAGTC 1 cut(s) 287
MmeI TCCRAC 1 cut(s) 28
MnlI CCTC 3 cut(s) 292, 373, 456
MseI TTAA 2 cut(s) 401, 428
MslI CAYNNNNRTG 1 cut(s) 326
MspI CCGG 1 cut(s) 391
MspR9I CCNGG 2 cut(s) 7, 392
MvaI CCWGG 1 cut(s) 7
MwoI GCNNNNNNNGC 1 cut(s) 472
NciI CCSGG 1 cut(s) 392
NdeII GATC 3 cut(s) 15, 19, 103
NlaIII CATG 2 cut(s) 227, 475
OliI CACNNNNGTG 1 cut(s) 326
PfeI GAWTC 1 cut(s) 499
PkrI GCNGC 2 cut(s) 370, 460
PleI GAGTC 1 cut(s) 286
PpsI GAGTC 1 cut(s) 286
Psp6I CCWGG 1 cut(s) 5
PspGI CCWGG 1 cut(s) 5
PspPI GGNCC 2 cut(s) 50, 161
PstI CTGCAG 1 cut(s) 328
PstNI CAGNNNCTG 1 cut(s) 107
PsuI RGATCY 1 cut(s) 103
RsaI GTAC 1 cut(s) 442
RsaNI GTAC 1 cut(s) 441
RseI CAYNNNNRTG 1 cut(s) 326
SaqAI TTAA 2 cut(s) 401, 428
SatI GCNGC 2 cut(s) 369, 459
Sau3AI GATC 3 cut(s) 15, 19, 103
Sau96I GGNCC 2 cut(s) 50, 161
SchI GAGTC 1 cut(s) 287
ScrFI CCNGG 2 cut(s) 7, 392
SetI ASST 8 cut(s) 127, 154, 166, 210, 384, 401, 460, 513
SfaNI GCATC 1 cut(s) 254
SfcI CTRYAG 2 cut(s) 324, 446
SinI GGWCC 2 cut(s) 50, 161
SmiMI CAYNNNNRTG 1 cut(s) 326
Sse9I AATT 4 cut(s) 175, 234, 346, 361
SspMI CTAG 1 cut(s) 213
StyD4I CCNGG 2 cut(s) 5, 390
TaaI ACNGT 1 cut(s) 307
TaqI TCGA 2 cut(s) 18, 492
TasI AATT 4 cut(s) 175, 234, 346, 361
TfiI GAWTC 1 cut(s) 499
Tru1I TTAA 2 cut(s) 401, 428
Tru9I TTAA 2 cut(s) 401, 428
TscAI CASTG 4 cut(s) 60, 328, 333, 348
TseI GCWGC 2 cut(s) 368, 458
TspDTI ATGAA 1 cut(s) 460
TspRI CASTG 4 cut(s) 60, 328, 333, 348
VpaK11BI GGWCC 2 cut(s) 50, 161
XagI CCTNNNNNAGG 1 cut(s) 10
XapI RAATTY 2 cut(s) 175, 346
XspI CTAG 1 cut(s) 213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.