pycom02g00480

NAD-specific glutamate dehydrogenase. Source PGD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0006960
Physical Location & Seq
Forward (+)
283310 .. 283513
204 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g00480.1

Sequence Viewer

Length: 204 bp
ATGCGGGTTCATCGCCACTTGGTTCTTCGCAGCGTCACCGATAAGCCGCTCGGTGTCGGTGAACGCCACATAGGACGGGGTTGTCCGGTTGCCTTGATCATTGGCGATGATCTCGACACGGTCGTTCTGCCAAACCCCAACGCAGCTGTAAGTTGTGCCGAGGTCGATGCCGATGGCCTTTCCTTCCGTCTTGGCCGCCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD-GH

Protein Analysis

68

Amino Acids

7.19

Weight (kDa)

6.89

Isoelectric Point (pI)

33.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD-GH PF10712 2 - 60 3.1e-06 NAD-specific glutamate dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 81
AccBSI CCGCTC 1 cut(s) 49
AciI CCGC 3 cut(s) 4, 47, 196
AcoI YGGCCR 1 cut(s) 193
AluBI AGCT 1 cut(s) 146
AluI AGCT 1 cut(s) 146
AoxI GGCC 2 cut(s) 175, 193
ApeKI GCWGC 2 cut(s) 30, 143
AsuHPI GGTGA 2 cut(s) 28, 71
BbvI GCAGC 2 cut(s) 42, 155
BccI CCATC 1 cut(s) 167
BcgI CGANNNNNNTGC 2 cut(s) 149, 183
BclI TGATCA 1 cut(s) 96
BisI GCNGC 4 cut(s) 31, 47, 144, 196
BlsI GCNGC 4 cut(s) 32, 48, 145, 197
BmsI GCATC 1 cut(s) 157
BsaJI CCNNGG 1 cut(s) 159
BsaWI WCCGGW 1 cut(s) 85
BseDI CCNNGG 1 cut(s) 159
BseXI GCAGC 2 cut(s) 42, 155
Bsh1285I CGRYCG 1 cut(s) 123
BshFI GGCC 2 cut(s) 177, 195
BsiEI CGRYCG 1 cut(s) 123
BsiSI CCGG 1 cut(s) 86
BsnI GGCC 2 cut(s) 177, 195
Bsp143I GATC 2 cut(s) 96, 109
BspACI CCGC 3 cut(s) 4, 47, 196
BspANI GGCC 2 cut(s) 177, 195
BsrBI CCGCTC 1 cut(s) 49
BssECI CCNNGG 1 cut(s) 159
BssMI GATC 2 cut(s) 96, 109
Bst4CI ACNGT 1 cut(s) 121
BstKTI GATC 2 cut(s) 99, 112
BstMBI GATC 2 cut(s) 96, 109
BstMCI CGRYCG 1 cut(s) 123
BstV1I GCAGC 2 cut(s) 42, 155
BsuRI GGCC 2 cut(s) 177, 195
BtgZI GCGATG 1 cut(s) 120
CseI GACGC 1 cut(s) 22
CviJI RGCY 4 cut(s) 46, 146, 177, 195
CviKI_1 RGCY 4 cut(s) 46, 146, 177, 195
DpnI GATC 2 cut(s) 98, 111
DpnII GATC 2 cut(s) 96, 109
DrdI GACNNNNNNGTC 1 cut(s) 81
DseDI GACNNNNNNGTC 1 cut(s) 81
EaeI YGGCCR 1 cut(s) 193
FaiI YATR 1 cut(s) 71
FbaI TGATCA 1 cut(s) 96
Fnu4HI GCNGC 4 cut(s) 31, 47, 144, 196
Fsp4HI GCNGC 4 cut(s) 31, 47, 144, 196
GluI GCNGC 4 cut(s) 31, 47, 144, 196
HaeIII GGCC 2 cut(s) 177, 195
HapII CCGG 1 cut(s) 86
HgaI GACGC 1 cut(s) 22
HpaII CCGG 1 cut(s) 86
HphI GGTGA 2 cut(s) 28, 71
Hpy166II GTNNAC 1 cut(s) 62
Hpy188III TCNNGA 1 cut(s) 113
Hpy8I GTNNAC 1 cut(s) 62
HpyAV CCTTC 1 cut(s) 193
HpyCH4III ACNGT 1 cut(s) 121
Ksp22I TGATCA 1 cut(s) 96
Kzo9I GATC 2 cut(s) 96, 109
LpnPI CCDG 1 cut(s) 99
Lsp1109I GCAGC 2 cut(s) 42, 155
LweI GCATC 1 cut(s) 157
MaeIII GTNAC 1 cut(s) 34
MalI GATC 2 cut(s) 98, 111
MbiI CCGCTC 1 cut(s) 49
MboI GATC 2 cut(s) 96, 109
MboII GAAGA 1 cut(s) 17
MnlI CCTC 1 cut(s) 154
MspA1I CMGCKG 1 cut(s) 146
MspI CCGG 1 cut(s) 86
NdeII GATC 2 cut(s) 96, 109
NmeAIII GCCGAG 1 cut(s) 184
NmuCI GTSAC 1 cut(s) 34
PcsI WCGNNNNNNNCGW 1 cut(s) 120
PflFI GACNNNGTC 1 cut(s) 119
PkrI GCNGC 4 cut(s) 32, 48, 145, 197
PsyI GACNNNGTC 1 cut(s) 119
PvuII CAGCTG 1 cut(s) 146
SatI GCNGC 4 cut(s) 31, 47, 144, 196
Sau3AI GATC 2 cut(s) 96, 109
SetI ASST 2 cut(s) 148, 165
SfaNI GCATC 1 cut(s) 157
SgeI CNNG 9 cut(s) 17, 31, 62, 89, 98, 106, 125, 130, 172
SsiI CCGC 3 cut(s) 4, 47, 196
TaaI ACNGT 1 cut(s) 121
TaqI TCGA 2 cut(s) 114, 165
TauI GCSGC 2 cut(s) 49, 198
TseFI GTSAC 1 cut(s) 34
TseI GCWGC 2 cut(s) 30, 143
Tsp45I GTSAC 1 cut(s) 34
TspGWI ACGGA 1 cut(s) 176
Tth111I GACNNNGTC 1 cut(s) 119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.