RLG00000035073

phosphoribosylformylglycinamidine synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0006960
Physical Location & Seq
Forward (+)
60298276 .. 60298752
477 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035073

Sequence Viewer

Length: 384 bp
ATGTTGGAGAAAGCAGCGATTAGGTGGCTTTACTCTTTCATGATTGTGAACAATTGCCCTGATCTTGAGGATGTTCTGTATCTGAAGCGAGTGTTTGAGGGAGTTCAGGGCCTTCTTGATGATGAACTGGTCTCTGGTGGCCATGATATTAGTGATTGTAGACTTCTGGTCTATGCCGTTGAGATGGCATTTGCTCGGAATTGTGGCATTAATTTGGACGAAAGTACCTTATTTCATAAAGGACATATGGGAGGAAGTGTTGGTGGTAGTGGCAGTGATGGGGGTGTTACAGCAGACTTTGGCTGCAACGTGGGTAGTGATGGTGGTGGCAATGGCGAGGATGGGGTTGAAGTGCTGCGGCGATGGGGAGGGGAGAAGAGATGA

Protein Analysis

128

Amino Acids

13.6

Weight (kDa)

4.61

Isoelectric Point (pI)

38.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AIRS_C PF02769 22 - 81 2.5e-06 AIR synthase related protein, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 160
AciI CCGC 1 cut(s) 358
AcoI YGGCCR 1 cut(s) 139
AcuI CTGAAG 1 cut(s) 104
AfaI GTAC 1 cut(s) 226
AgsI TTSAA 1 cut(s) 350
AhdI GACNNNNNGTC 1 cut(s) 167
Alw26I GTCTC 1 cut(s) 136
AoxI GGCC 2 cut(s) 109, 139
ApeKI GCWGC 3 cut(s) 14, 303, 355
AseI ATTAAT 1 cut(s) 210
AspS9I GGNCC 1 cut(s) 109
BaeI ACNNNNGTAYC 2 cut(s) 208, 241
BalI TGGCCA 1 cut(s) 141
BbvI GCAGC 3 cut(s) 26, 290, 342
BccI CCATC 5 cut(s) 178, 272, 314, 335, 357
BceAI ACGGC 1 cut(s) 161
BcoDI GTCTC 1 cut(s) 136
BisI GCNGC 4 cut(s) 15, 304, 356, 359
BlsI GCNGC 4 cut(s) 16, 305, 357, 360
BmeRI GACNNNNNGTC 1 cut(s) 167
BmgT120I GGNCC 1 cut(s) 109
BpuEI CTTGAG 1 cut(s) 86
BsaI GGTCTC 1 cut(s) 136
BsaXI ACNNNNNCTCC 2 cut(s) 243, 273
Bse1I ACTGG 1 cut(s) 132
Bse3DI GCAATG 1 cut(s) 337
BseGI GGATG 2 cut(s) 76, 346
BseMI GCAATG 1 cut(s) 337
BseNI ACTGG 1 cut(s) 132
BseXI GCAGC 3 cut(s) 26, 290, 342
BshFI GGCC 2 cut(s) 111, 141
BsmAI GTCTC 1 cut(s) 136
BsnI GGCC 2 cut(s) 111, 141
Bso31I GGTCTC 1 cut(s) 136
Bsp143I GATC 1 cut(s) 61
BspACI CCGC 1 cut(s) 358
BspANI GGCC 2 cut(s) 111, 141
BspHI TCATGA 1 cut(s) 39
BspTNI GGTCTC 1 cut(s) 136
BsrDI GCAATG 1 cut(s) 337
BsrI ACTGG 1 cut(s) 132
BssMI GATC 1 cut(s) 61
Bst6I CTCTTC 1 cut(s) 371
BstF5I GGATG 2 cut(s) 76, 346
BstKTI GATC 1 cut(s) 64
BstMAI GTCTC 1 cut(s) 136
BstMBI GATC 1 cut(s) 61
BstV1I GCAGC 3 cut(s) 26, 290, 342
BsuRI GGCC 2 cut(s) 111, 141
BtgZI GCGATG 1 cut(s) 376
BtsCI GGATG 2 cut(s) 76, 346
BtsI GCAGTG 1 cut(s) 280
BtsIMutI CAGTG 1 cut(s) 280
CciI TCATGA 1 cut(s) 39
Cfr13I GGNCC 1 cut(s) 109
Csp6I GTAC 1 cut(s) 225
CviAII CATG 2 cut(s) 40, 143
CviJI RGCY 4 cut(s) 28, 111, 141, 303
CviKI_1 RGCY 4 cut(s) 28, 111, 141, 303
CviQI GTAC 1 cut(s) 225
DpnI GATC 1 cut(s) 63
DpnII GATC 1 cut(s) 61
DriI GACNNNNNGTC 1 cut(s) 167
EaeI YGGCCR 1 cut(s) 139
Eam1104I CTCTTC 1 cut(s) 371
Eam1105I GACNNNNNGTC 1 cut(s) 167
EarI CTCTTC 1 cut(s) 371
Eco31I GGTCTC 1 cut(s) 136
Eco57I CTGAAG 1 cut(s) 104
EcoO109I RGGNCCY 1 cut(s) 109
FaeI CATG 2 cut(s) 43, 146
FaiI YATR 6 cut(s) 41, 144, 174, 237, 246, 248
FatI CATG 2 cut(s) 39, 142
FauNDI CATATG 1 cut(s) 246
FblI GTMKAC 1 cut(s) 160
Fnu4HI GCNGC 4 cut(s) 15, 304, 356, 359
FokI GGATG 2 cut(s) 83, 353
Fsp4HI GCNGC 4 cut(s) 15, 304, 356, 359
GluI GCNGC 4 cut(s) 15, 304, 356, 359
HaeIII GGCC 2 cut(s) 111, 141
Hin1II CATG 2 cut(s) 43, 146
Hpy166II GTNNAC 2 cut(s) 49, 161
Hpy188I TCNGA 2 cut(s) 84, 198
Hpy188III TCNNGA 3 cut(s) 40, 65, 116
Hpy8I GTNNAC 2 cut(s) 49, 161
HpyAV CCTTC 1 cut(s) 122
HpyCH4IV ACGT 1 cut(s) 309
HpyCH4V TGCA 1 cut(s) 306
HpySE526I ACGT 1 cut(s) 309
Hsp92II CATG 2 cut(s) 43, 146
Kzo9I GATC 1 cut(s) 61
LpnPI CCDG 5 cut(s) 72, 92, 113, 120, 152
Lsp1109I GCAGC 3 cut(s) 26, 290, 342
MaeII ACGT 1 cut(s) 309
MaeIII GTNAC 1 cut(s) 286
MalI GATC 1 cut(s) 63
MboI GATC 1 cut(s) 61
MfeI CAATTG 1 cut(s) 52
MlsI TGGCCA 1 cut(s) 141
MluCI AATT 3 cut(s) 52, 199, 211
MluNI TGGCCA 1 cut(s) 141
MnlI CCTC 5 cut(s) 61, 91, 245, 331, 362
Mox20I TGGCCA 1 cut(s) 141
MscI TGGCCA 1 cut(s) 141
MseI TTAA 1 cut(s) 210
MslI CAYNNNNRTG 1 cut(s) 44
Msp20I TGGCCA 1 cut(s) 141
MunI CAATTG 1 cut(s) 52
NdeI CATATG 1 cut(s) 246
NdeII GATC 1 cut(s) 61
NlaIII CATG 2 cut(s) 43, 146
PagI TCATGA 1 cut(s) 39
PkrI GCNGC 4 cut(s) 16, 305, 357, 360
PshBI ATTAAT 1 cut(s) 210
PspPI GGNCC 1 cut(s) 109
RsaI GTAC 1 cut(s) 226
RsaNI GTAC 1 cut(s) 225
RseI CAYNNNNRTG 1 cut(s) 44
SaqAI TTAA 1 cut(s) 210
SatI GCNGC 4 cut(s) 15, 304, 356, 359
Sau3AI GATC 1 cut(s) 61
Sau96I GGNCC 1 cut(s) 109
SetI ASST 3 cut(s) 26, 230, 312
SmiMI CAYNNNNRTG 1 cut(s) 44
SmlI CTYRAG 1 cut(s) 65
SmoI CTYRAG 1 cut(s) 65
Sse9I AATT 3 cut(s) 52, 199, 211
SsiI CCGC 1 cut(s) 358
TaiI ACGT 1 cut(s) 312
TasI AATT 3 cut(s) 52, 199, 211
TauI GCSGC 1 cut(s) 361
Tru1I TTAA 1 cut(s) 210
Tru9I TTAA 1 cut(s) 210
TscAI CASTG 1 cut(s) 280
TseI GCWGC 3 cut(s) 14, 303, 355
TspDTI ATGAA 3 cut(s) 28, 138, 224
TspRI CASTG 1 cut(s) 280
VspI ATTAAT 1 cut(s) 210
XmiI GTMKAC 1 cut(s) 160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.