pycom02g05480

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0002500
Physical Location & Seq
Reverse (-)
3668800 .. 3676426
7627 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4017 bp
ATGGCTCAAAGCGCCCCGCTTCTTCTCCACCTTTTCATCCTCAACCTCTTCATCAGCTTCGCCATAAACCCTAGCCTCTGCGCTGATTCCGACGATGAATTTTCAATTCTCGCGTTCGAAATTCACGGGGACTATTCGCCTCCTTCTCCGCCGCCGGTTCCGCCGCCGCCCCACCCTCCCCCGCTCTCGTGTCAGGGGGGTCTCAATGGAATTGGGTCGCTGGACACCATTTGTGAGCTGAATTCCAGTTTGATTCTTGAAGATAATGTGTATATAGAAGGAAATGGGAGCTTATTTATACTTTCCGGTGTTAATTTGAGCTGCCCATTTTTGGGTTGCGAAATTGTGGTTAACATTAGTGGGGAATTCAGTTTAGGTAGAAATTCAATGATTATTGCCGGGTTAGTTACGGTTAATGCTTCGAATGCGAGCTTTTTAACTGGTTCAGTGGTAAATGTTACGGCATTGGCGGGTGCACCCCCTGCGCAGACTAGTGGCACCCCGGACGGTGTTCAGGGTTCTGGTGGAGGGCATGGCGGGAGAGGTGCTAGTTGTGTGATTGATAATACGAAGCTCCCGGACGATGTTTGGGGTGGGGATCCGTATGCATGGTCATCGTTAAGTGAGCCGGTGAGTTATGGGAGTAAGGGTGGAACAACTAGTAAGGATGAGAAGTATGGTGGGGAAGGAGGTGGGAGGATTTGGTTGGAGGCAAGAAGTTCAATTGAACTTAGTGGGAGTGTTTTGGCTGATGGCGGTGATGGTGGGATTAAGGGTGGTGGAGGGTCAGGGGGCAGCATATTTGTCAAAGCTCGTAGAATGATAAGTGCAGTGGGGGGTAATGGATTTGCTGGAGGGGGAGGTGGAAGAGTTTCCATCAAAGTTTTCAGCAGGCGTGATAATACAGATATCTTTGCTCATGGGGGAAGGAGTTTAGGCTGTCTGGAAAATGCAGGTGCTGCAGGGACATATTATGATGCTGTACCTCGGAGACTTATAGTCAATAATCATAACTTGTCCACACAGACTGACACGCTTCTGTTAGAGTTTCCCAAACAGCCACTTTGGACAAATGTTGATATCCAAAATCATGCCAAGGCTTTGGTTCCTTTGTATTGGAGCCGTGTTCAGGTTCGAGGTCAAATTCGTTTATCATGTGGTGCAGTTTTGAGTTTTGGTCTTGCACATTTTGCTTCATCAGAATTTGAGTTAATGGCAGAAGAGCTTCTGATGAGTGATTCTGTTATCAAGATATTTGGGGCTCTTCGAATGTCTGTCAAAATGCACTTGATGTGGAATTCTAAAATGCTTATAGATGGTCTTGCTGATGCAATTGTGGCAACATCCTTGCTTGAGGCAAGCAATGTAGTGGTTCTCAGGATCTGTCGAGTTGAAGATGTTATTATTCAAGGCAGTATAACTGGATCTGTTATACATTTTCACTGGGTTAGAGCTGTAGAGGTCCACTCTTCTGGGGTAATTAGTGCATCTGGCCTAGGCTGCCCTGGTGGGATTGGTAGAGGAAAGTTTTTCACAAATGGGCTTGGTGGCGGTGGTGGGCATGGTGGCAAAGGTGGGGATGGGTATTACGATGGAAATTTTATTGACGGTGGTGTTTCCTATGGAGATGCTGATTTGCCGTGTGAACTTGGTAGTGGTAGTGGAAATGATAGCCTAGCTGGTGCAACTGCAGGTGGTGGTATCATTGTAATGGGTTCATTGGAGCGCTCATTGTCAAGTTTGTCTCTTGATGGTTCACTTAGATCCGACGGAGAATCCTTTGGAGAAAAATATTTGGAGAAAAATGATAGGGTCTTTACAAATATAGGTCCTGGGGGTGGTTCTGGTGGAACTATTCTATTGTTTGTTCAAACATTGGCACTTGGTAATTCTTCTACAATCTCAACTGTTGGTGGACATGGTAGTCCTAGTGGTGGTGGCGGAGGAGGTGGTGGAAGGATTCACTTTCACTGGTCAGATATACCAGTTGGGGATGCATATCTACCCACAGCAAGTGTGAATGGAAGCATCATTACTGGGGGAGGCTTCGGTAGAGGTCATGGTCAGGCTGGACAAAATGGGACTGTTACTGGAAAGGCCTGTCCCAGAGGGCTTTATGGTATCTTTTGTGAGGAATGCCCTGTTGGCACCTTTAAGAATGTGAGTGGCTCTGATAGAGCCCTTTGTCATGCTTGCCCATCTTTGGAGCTTCCACATCGTGCCATATATGTCATGGTTCGAGGTGGTGTCACCGAAACTCCATGTCCGCATAAGTGCATTTCTGACAGATATCACATGCCTAAGTGTTATACAGCATTTGAAGAGTTGGTTTATACTTTTGGCGGACCATGGTGGTTTGGTTTTATTCTGTTAAGCATCCTCATCCTGTTAGCTCTTGTGCTTAGTGTTGCACGGATGAAGTATGTTACTGGAGACGAATTACCGACTCCTCTACCTGCTCGACAGGGCTCTCGTTTAGATAATTCCTTCCCTTTCCTGGAATCGTTGAATGAGGTTTTGGAAACAAATAGAAACGAGGAATCTCAGAGTCATGTGCATAGGATGTTTTTCATGGGGCCAAATACATTCAGTGAACCTTGGCATCTACCTCATTCCCCTCCAGAACAAGTCACAGAGATTGTGTATGAGGCTGCATTCAATAGATTTGTGGACGAGATAAATGGTTTAGCTGCTTATCAGTGGTGGGAAGGATCAGTCTACAGCATTCTTTGCGTGTTTGCCTATCCACTTGCCTGGTCATGGTTACAGAGTCGAAGGAAAAAGAAGTTACAACAACTTCGTGAATATGTTAGATCAGAATATGATCATTCGTGTTTGCGTTCTTGTCGTTCACGTGCACTGTATGAAGGACTCAAGGTAGCTGCAACTTCTGATTTAATGCTTGCATACGTGGACTTTTACCTTGGTGGAGATGAGAAAAGAGCTGGTCTTCCTCCCCGTCTTCATCAGCGATTTCCATTGTCATTAATTTTTGGAGGAGATGGAAGTTACATGGCGCCTTTCCATCTTTGCAGTGATAACATTCTCACTAGCCTCATGAGTCAGAGTATTCCACCTACCATCTGGTATCGGCTTGTAGCTGGTCTAAATGCTCAACTGCGATTGGTTCGGCGTGGTCACTTAAAGTTAACTTTTGGTCTTGTTATCAGCTGGCTTGAAACGCATGCTAACCCTACTCTTAGGCCATATGGCGTACATGTTGATCTTGCCTGGTTTCAGCCAACAGCGTCTGGGTATAGCCAGTTTGGACTTTTGATATATGCTATCGACAACGAAAGTGTGCCACCAACACTGGAGGCTCAAGATGCATTGTTGCCACGGGATTATCTGTCACGTACGCCAAGAACTCATTGGGAAAACCCATTTGAGCATTTGAGACTCATCGACCATTGGATGTCACAGAAAAGATTTTCTGGAGGCATTTTACACAGCAAGAGCTTAAGGACATTTAAAGAGAAGACAGCTATATTTTATCCGTATTCTTTCATAGTTTATAATGGCAAACCGGTTGGCCATCAGGATCTTGTCGGTTTGTTCATCTCTATTCTACTCTTGGGAGATTTTAGCATAGTCTTGCTCAGTTTGCTGCAGCTATATTCTATTTCACTGTTGAACTTTTTCTTAGTCTTATTTATGCTTCCTCTCGGTCTACTGTTCCCCTTCCCGGCTGGAATCAGTGCTTTGTTTAGTCGTGGACCTAAACGGGCAGCAGGTCTTACGCGTATATATGCTTTATGGAACATCACATCCTTGATCAATGTCGGTGTTGCATTTACGTGTGGATTGATTCATTATGCGGCCCACTCGAGGAAAAAGCACTCCAACTTTCAGTCCTGGAATTTTAGCATGGATGAAAGTGAATGGTGGGTGCTTCCCTGTGGCCTGGCACTGTGTAAACTCGTGCAGTCGCGGCTCATCGACTGCCATGTGGCGAACCAGGAAATCCAGGATCACTCATTGTACAGCAATGACCCCGACGTTTTCTGGCAGTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1339

Amino Acids

144.13

Weight (kDa)

6.22

Isoelectric Point (pI)

45.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8003 PF26010 696 - 770 7.9e-35 Domain of unknown function (DUF8003)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3516
AarI CACCTGC 2 cut(s) 944, 1682
AasI GACNNNNNNGTC 1 cut(s) 1923
Acc16I TGCGCA 1 cut(s) 486
Acc36I ACCTGC 4 cut(s) 944, 1682, 2464, 3722
AccB1I GGYRCC 3 cut(s) 497, 2147, 3016
AccBSI CCGCTC 1 cut(s) 184
AccI GTMKAC 2 cut(s) 2718, 3670
AccII CGCG 3 cut(s) 113, 3742, 3931
AclWI GGATC 8 cut(s) 593, 606, 1388, 1432, 1758, 2719, 3549, 3978
AcoI YGGCCR 1 cut(s) 3532
AcvI CACGTG 1 cut(s) 2855
AcyI GRCGYC 1 cut(s) 3017
AdeI CACNNNGTG 1 cut(s) 2219
AfaI GTAC 4 cut(s) 984, 3216, 3358, 3983
AfeI AGCGCT 1 cut(s) 1727
AflII CTTAAG 1 cut(s) 3460
AflIII ACRYGT 3 cut(s) 3217, 3740, 3797
AgeI ACCGGT 1 cut(s) 3526
AhdI GACNNNNNGTC 1 cut(s) 998
AhlI ACTAGT 2 cut(s) 491, 659
AjnI CCWGG 9 cut(s) 1504, 1831, 2496, 2753, 3230, 3854, 3903, 3957, 3966
AjuI GAANNNNNNNTTGG 4 cut(s) 2127, 2159, 2501, 2533
AloI GAACNNNNNNTCC 2 cut(s) 3752, 3784
Alw21I GWGCWC 2 cut(s) 478, 2860
Alw26I GTCTC 5 cut(s) 206, 985, 1749, 2427, 3391
Alw44I GTGCAC 2 cut(s) 474, 2856
AlwI GGATC 8 cut(s) 593, 606, 1388, 1432, 1758, 2719, 3549, 3978
AlwNI CAGNNNCTG 3 cut(s) 959, 1383, 3251
Ama87I CYCGRG 1 cut(s) 3826
Aor51HI AGCGCT 1 cut(s) 1727
AoxI GGCC 7 cut(s) 1492, 2097, 2576, 3203, 3532, 3819, 3901
ApaLI GTGCAC 2 cut(s) 474, 2856
AseI ATTAAT 1 cut(s) 2987
AsiGI ACCGGT 1 cut(s) 3526
Asp700I GAANNNNTTC 3 cut(s) 871, 1224, 3638
AspA2I CCTAGG 1 cut(s) 1495
AspLEI GCGC 5 cut(s) 14, 83, 487, 1728, 3019
AspS9I GGNCC 6 cut(s) 1462, 1829, 2345, 2576, 3716, 3820
AsuC2I CCSGG 4 cut(s) 400, 503, 578, 3686
AsuHPI GGTGA 3 cut(s) 643, 770, 2242
AsuII TTCGAA 3 cut(s) 117, 422, 1267
AvaI CYCGRG 1 cut(s) 3826
AvaII GGWCC 4 cut(s) 1462, 1829, 2345, 3716
AvrII CCTAGG 1 cut(s) 1495
BaeGI GKGCMC 2 cut(s) 478, 2860
BalI TGGCCA 1 cut(s) 3534
BamHI GGATCC 1 cut(s) 598
BanI GGYRCC 3 cut(s) 497, 2147, 3016
BanII GRGCYC 3 cut(s) 1264, 2182, 2471
BarI GAAGNNNNNNTAC 2 cut(s) 2017, 2049
BauI CACGAG 2 cut(s) 187, 3920
BbrPI CACGTG 1 cut(s) 2855
BbsI GAAGAC 3 cut(s) 2942, 2954, 3485
Bbv12I GWGCWC 2 cut(s) 478, 2860
BceAI ACGGC 3 cut(s) 477, 1107, 1624
BcgI CGANNNNNNTGC 2 cut(s) 597, 631
BciT130I CCWGG 9 cut(s) 1506, 1833, 2498, 2755, 3232, 3856, 3905, 3959, 3968
BclI TGATCA 2 cut(s) 2824, 3774
BcnI CCSGG 4 cut(s) 400, 503, 578, 3686
BcoDI GTCTC 5 cut(s) 206, 985, 1749, 2427, 3391
BcuI ACTAGT 2 cut(s) 491, 659
BfaI CTAG 8 cut(s) 72, 492, 549, 660, 1496, 1676, 1929, 3051
BfmI CTRYAG 5 cut(s) 960, 1455, 1689, 2719, 3608
BfoI RGCGCY 3 cut(s) 15, 1729, 3020
BfrI CTTAAG 1 cut(s) 3460
BfuAI ACCTGC 4 cut(s) 944, 1682, 2464, 3722
BglI GCCNNNNNGGC 1 cut(s) 2145
BlnI CCTAGG 1 cut(s) 1495
Bme18I GGWCC 4 cut(s) 1462, 1829, 2345, 3716
BmeRI GACNNNNNGTC 1 cut(s) 998
BmeT110I CYCGRG 1 cut(s) 3826
BmgT120I GGNCC 6 cut(s) 1462, 1829, 2345, 2576, 3716, 3820
BmiI GGNNCC 8 cut(s) 159, 499, 600, 1107, 1121, 2149, 2577, 3018
BmrI ACTGGG 2 cut(s) 1453, 2046
BmsI GCATC 9 cut(s) 967, 1318, 1496, 1618, 1984, 2037, 2385, 2611, 3316
BmuI ACTGGG 2 cut(s) 1453, 2046
BpiI GAAGAC 3 cut(s) 2942, 2954, 3485
BplI GAGNNNNNCTC 2 cut(s) 1451, 1483
BpmI CTGGAG 5 cut(s) 873, 2451, 2604, 3335, 3456
Bpu14I TTCGAA 3 cut(s) 117, 422, 1267
BpuEI CTTGAG 3 cut(s) 1373, 2858, 3306
BpuMI CCSGG 4 cut(s) 400, 503, 578, 3686
BsaAI YACGTR 4 cut(s) 2855, 2911, 3356, 3798
BsaBI GATNNNNATC 1 cut(s) 1998
BsaHI GRCGYC 1 cut(s) 3017
BsaI GGTCTC 1 cut(s) 206
BsaWI WCCGGW 2 cut(s) 305, 3526
BsaXI ACNNNNNCTCC 4 cut(s) 634, 664, 2242, 2272
Bse118I RCCGGY 3 cut(s) 154, 628, 3526
Bse3DI GCAATG 2 cut(s) 1369, 3994
Bse8I GATNNNNATC 1 cut(s) 1998
BseBI CCWGG 9 cut(s) 1506, 1833, 2498, 2755, 3232, 3856, 3905, 3959, 3968
BseJI GATNNNNATC 1 cut(s) 1998
BseMI GCAATG 2 cut(s) 1369, 3994
BseMII CTCAG 3 cut(s) 1390, 2558, 3613
BseRI GAGGAG 3 cut(s) 1959, 2439, 3012
BseSI GKGCMC 2 cut(s) 478, 2860
BsgI GTGCAG 3 cut(s) 849, 1182, 3944
Bsh1236I CGCG 3 cut(s) 113, 3742, 3931
BshFI GGCC 7 cut(s) 1494, 2099, 2578, 3205, 3534, 3821, 3903
BshNI GGYRCC 3 cut(s) 497, 2147, 3016
BshTI ACCGGT 1 cut(s) 3526
BsiHKAI GWGCWC 2 cut(s) 478, 2860
BsiHKCI CYCGRG 1 cut(s) 3826
BsiSI CCGG 8 cut(s) 155, 306, 399, 503, 578, 629, 3527, 3686
BsiWI CGTACG 1 cut(s) 3356
BslFI GGGAC 4 cut(s) 143, 979, 2088, 2095
BsmAI GTCTC 5 cut(s) 206, 985, 1749, 2427, 3391
BsmBI CGTCTC 1 cut(s) 2427
BsmFI GGGAC 4 cut(s) 143, 979, 2088, 2095
BsmI GAATGC 4 cut(s) 430, 2141, 2654, 2724
BsnI GGCC 7 cut(s) 1494, 2099, 2578, 3205, 3534, 3821, 3903
Bso31I GGTCTC 1 cut(s) 206
BsoBI CYCGRG 1 cut(s) 3826
Bsp119I TTCGAA 3 cut(s) 117, 422, 1267
Bsp1286I GDGCHC 5 cut(s) 478, 1264, 2182, 2471, 2860
Bsp1407I TGTACA 1 cut(s) 3981
Bsp19I CCATGG 1 cut(s) 2348
BspANI GGCC 7 cut(s) 1494, 2099, 2578, 3205, 3534, 3821, 3903
BspCNI CTCAG 3 cut(s) 1389, 2557, 3612
BspFNI CGCG 3 cut(s) 113, 3742, 3931
BspHI TCATGA 2 cut(s) 3057, 4013
BspLI GGNNCC 8 cut(s) 159, 499, 600, 1107, 1121, 2149, 2577, 3018
BspMAI CTGCAG 3 cut(s) 964, 1693, 3612
BspMI ACCTGC 4 cut(s) 944, 1682, 2464, 3722
BspPI GGATC 8 cut(s) 593, 606, 1388, 1432, 1758, 2719, 3549, 3978
BspQI GCTCTTC 2 cut(s) 1215, 1269
BspT104I TTCGAA 3 cut(s) 117, 422, 1267
BspT107I GGYRCC 3 cut(s) 497, 2147, 3016
BspTI CTTAAG 1 cut(s) 3460
BspTNI GGTCTC 1 cut(s) 206
BsrBI CCGCTC 1 cut(s) 184
BsrDI GCAATG 2 cut(s) 1369, 3994
BsrFI RCCGGY 3 cut(s) 154, 628, 3526
BsrGI TGTACA 1 cut(s) 3981
BssAI RCCGGY 3 cut(s) 154, 628, 3526
BssNI GRCGYC 1 cut(s) 3017
BssSI CACGAG 2 cut(s) 187, 3920
BssT1I CCWWGG 5 cut(s) 1095, 1495, 2348, 2597, 2923
Bst2BI CACGAG 2 cut(s) 187, 3920
Bst2UI CCWGG 9 cut(s) 1506, 1833, 2498, 2755, 3232, 3856, 3905, 3959, 3968
Bst4CI ACNGT 9 cut(s) 412, 509, 1610, 1909, 2086, 2862, 3630, 3675, 3912
Bst6I CTCTTC 6 cut(s) 53, 862, 1215, 1269, 1474, 2316
BstACI GRCGYC 1 cut(s) 3017
BstAFI CTTAAG 1 cut(s) 3460
BstAPI GCANNNNNTGC 4 cut(s) 482, 959, 1190, 2730
BstAUI TGTACA 1 cut(s) 3981
BstBAI YACGTR 4 cut(s) 2855, 2911, 3356, 3798
BstBI TTCGAA 3 cut(s) 117, 422, 1267
BstC8I GCNNGC 7 cut(s) 430, 893, 1360, 2194, 2904, 3173, 3186
BstDEI CTNAG 9 cut(s) 731, 1376, 1760, 2301, 2402, 2544, 3200, 3599, 3643
BstDSI CCRYGG 2 cut(s) 2348, 3338
BstFNI CGCG 3 cut(s) 113, 3742, 3931
BstH2I RGCGCY 3 cut(s) 15, 1729, 3020
BstHHI GCGC 5 cut(s) 14, 83, 487, 1728, 3019
BstMAI GTCTC 5 cut(s) 206, 985, 1749, 2427, 3391
BstNI CCWGG 9 cut(s) 1506, 1833, 2498, 2755, 3232, 3856, 3905, 3959, 3968
BstNSI RCATGY 3 cut(s) 2299, 3188, 3221
BstSFI CTRYAG 5 cut(s) 960, 1455, 1689, 2719, 3608
BstSLI GKGCMC 2 cut(s) 478, 2860
BstUI CGCG 3 cut(s) 113, 3742, 3931
BstV2I GAAGAC 3 cut(s) 2942, 2954, 3485
BstX2I RGATCY 5 cut(s) 598, 1380, 1424, 1763, 3541
BstXI CCANNNNNNTGG 3 cut(s) 1102, 1472, 2754
BstYI RGATCY 5 cut(s) 598, 1380, 1424, 1763, 3541
BsuRI GGCC 7 cut(s) 1494, 2099, 2578, 3205, 3534, 3821, 3903
BtgI CCRYGG 2 cut(s) 2348, 3338
BtsI GCAGTG 2 cut(s) 837, 3040
BveI ACCTGC 4 cut(s) 944, 1682, 2464, 3722
Cac8I GCNNGC 7 cut(s) 430, 893, 1360, 2194, 2904, 3173, 3186
CaiI CAGNNNCTG 3 cut(s) 959, 1383, 3251
CciI TCATGA 2 cut(s) 3057, 4013
CfoI GCGC 5 cut(s) 14, 83, 487, 1728, 3019
Cfr10I RCCGGY 3 cut(s) 154, 628, 3526
Cfr13I GGNCC 6 cut(s) 1462, 1829, 2345, 2576, 3716, 3820
CseI GACGC 1 cut(s) 3237
Csp6I GTAC 4 cut(s) 983, 3215, 3357, 3982
CspAI ACCGGT 1 cut(s) 3526
CviQI GTAC 4 cut(s) 983, 3215, 3357, 3982
DdeI CTNAG 9 cut(s) 731, 1376, 1760, 2301, 2402, 2544, 3200, 3599, 3643
DinI GGCGCC 1 cut(s) 3018
DraI TTTAAA 1 cut(s) 3472
DraIII CACNNNGTG 1 cut(s) 2219
DrdI GACNNNNNNGTC 1 cut(s) 1923
DriI GACNNNNNGTC 1 cut(s) 998
DseDI GACNNNNNNGTC 1 cut(s) 1923
EaeI YGGCCR 1 cut(s) 3532
Eam1104I CTCTTC 6 cut(s) 53, 862, 1215, 1269, 1474, 2316
Eam1105I GACNNNNNGTC 1 cut(s) 998
EarI CTCTTC 6 cut(s) 53, 862, 1215, 1269, 1474, 2316
EciI GGCGGA 4 cut(s) 138, 150, 1956, 2358
Eco130I CCWWGG 5 cut(s) 1095, 1495, 2348, 2597, 2923
Eco147I AGGCCT 1 cut(s) 2099
Eco24I GRGCYC 3 cut(s) 1264, 2182, 2471
Eco31I GGTCTC 1 cut(s) 206
Eco32I GATATC 3 cut(s) 910, 1081, 2291
Eco47I GGWCC 4 cut(s) 1462, 1829, 2345, 3716
Eco47III AGCGCT 1 cut(s) 1727
Eco72I CACGTG 1 cut(s) 2855
Eco88I CYCGRG 1 cut(s) 3826
EcoO109I RGGNCCY 1 cut(s) 1829
EcoRI GAATTC 3 cut(s) 241, 365, 1297
EcoRII CCWGG 9 cut(s) 1504, 1831, 2496, 2753, 3230, 3854, 3903, 3957, 3966
EcoRV GATATC 3 cut(s) 910, 1081, 2291
EcoT14I CCWWGG 5 cut(s) 1095, 1495, 2348, 2597, 2923
EcoT22I ATGCAT 3 cut(s) 610, 1999, 3331
EcoT38I GRGCYC 3 cut(s) 1264, 2182, 2471
EgeI GGCGCC 1 cut(s) 3018
EheI GGCGCC 1 cut(s) 3018
ErhI CCWWGG 5 cut(s) 1095, 1495, 2348, 2597, 2923
Esp3I CGTCTC 1 cut(s) 2427
FaqI GGGAC 4 cut(s) 143, 979, 2088, 2095
FauI CCCGC 4 cut(s) 24, 189, 463, 530
FauNDI CATATG 1 cut(s) 3208
FbaI TGATCA 2 cut(s) 2824, 3774
FblI GTMKAC 2 cut(s) 2718, 3670
FriOI GRGCYC 3 cut(s) 1264, 2182, 2471
FspBI CTAG 8 cut(s) 72, 492, 549, 660, 1496, 1676, 1929, 3051
FspI TGCGCA 1 cut(s) 486
GlaI GCGC 5 cut(s) 13, 82, 486, 1727, 3018
GsuI CTGGAG 5 cut(s) 873, 2451, 2604, 3335, 3456
HaeII RGCGCY 3 cut(s) 15, 1729, 3020
HaeIII GGCC 7 cut(s) 1494, 2099, 2578, 3205, 3534, 3821, 3903
HapII CCGG 8 cut(s) 155, 306, 399, 503, 578, 629, 3527, 3686
HgaI GACGC 1 cut(s) 3237
HhaI GCGC 5 cut(s) 14, 83, 487, 1728, 3019
Hin1I GRCGYC 1 cut(s) 3017
Hin6I GCGC 5 cut(s) 12, 81, 485, 1726, 3017
HinP1I GCGC 5 cut(s) 12, 81, 485, 1726, 3017
HincII GTYRAC 2 cut(s) 352, 3150
HindII GTYRAC 2 cut(s) 352, 3150
HpaI GTTAAC 2 cut(s) 352, 3150
HpaII CCGG 8 cut(s) 155, 306, 399, 503, 578, 629, 3527, 3686
HphI GGTGA 3 cut(s) 643, 770, 2242
Hpy99I CGWCG 3 cut(s) 95, 1772, 4001
HpyCH4III ACNGT 9 cut(s) 412, 509, 1610, 1909, 2086, 2862, 3630, 3675, 3912
HpyCH4IV ACGT 5 cut(s) 2854, 2910, 3355, 3797, 3999
HpyF3I CTNAG 9 cut(s) 731, 1376, 1760, 2301, 2402, 2544, 3200, 3599, 3643
HpySE526I ACGT 5 cut(s) 2854, 2910, 3355, 3797, 3999
Hsp92I GRCGYC 1 cut(s) 3017
HspAI GCGC 5 cut(s) 12, 81, 485, 1726, 3017
KasI GGCGCC 1 cut(s) 3016
Ksp22I TGATCA 2 cut(s) 2824, 3774
KspAI GTTAAC 2 cut(s) 352, 3150
LguI GCTCTTC 2 cut(s) 1215, 1269
LmnI GCTCC 5 cut(s) 288, 579, 1119, 1723, 2206
LweI GCATC 9 cut(s) 967, 1318, 1496, 1618, 1984, 2037, 2385, 2611, 3316
MaeI CTAG 8 cut(s) 72, 492, 549, 660, 1496, 1676, 1929, 3051
MaeII ACGT 5 cut(s) 2854, 2910, 3355, 3797, 3999
MbiI CCGCTC 1 cut(s) 184
MfeI CAATTG 2 cut(s) 723, 1332
MflI RGATCY 5 cut(s) 598, 1380, 1424, 1763, 3541
MhlI GDGCHC 5 cut(s) 478, 1264, 2182, 2471, 2860
MlsI TGGCCA 1 cut(s) 3534
MluI ACGCGT 1 cut(s) 3740
MluNI TGGCCA 1 cut(s) 3534
Mly113I GGCGCC 1 cut(s) 3017
MlyI GAGTC 6 cut(s) 2440, 2557, 2779, 2865, 3070, 3393
MmeI TCCRAC 4 cut(s) 114, 687, 1791, 3867
Mox20I TGGCCA 1 cut(s) 3534
Mph1103I ATGCAT 3 cut(s) 610, 1999, 3331
MroXI GAANNNNTTC 3 cut(s) 871, 1224, 3638
MscI TGGCCA 1 cut(s) 3534
MslI CAYNNNNRTG 2 cut(s) 1709, 3796
Msp20I TGGCCA 1 cut(s) 3534
MspA1I CMGCKG 1 cut(s) 3171
MspCI CTTAAG 1 cut(s) 3460
MspI CCGG 8 cut(s) 155, 306, 399, 503, 578, 629, 3527, 3686
MunI CAATTG 2 cut(s) 723, 1332
Mva1269I GAATGC 4 cut(s) 430, 2141, 2654, 2724
MvaI CCWGG 9 cut(s) 1506, 1833, 2498, 2755, 3232, 3856, 3905, 3959, 3968
MvnI CGCG 3 cut(s) 113, 3742, 3931
NarI GGCGCC 1 cut(s) 3017
NciI CCSGG 4 cut(s) 400, 503, 578, 3686
NcoI CCATGG 1 cut(s) 2348
NdeI CATATG 1 cut(s) 3208
NlaIV GGNNCC 8 cut(s) 159, 499, 600, 1107, 1121, 2149, 2577, 3018
NmuCI GTSAC 5 cut(s) 2248, 2629, 3137, 3351, 3417
NsbI TGCGCA 1 cut(s) 486
NsiI ATGCAT 3 cut(s) 610, 1999, 3331
NspI RCATGY 3 cut(s) 2299, 3188, 3221
NspV TTCGAA 3 cut(s) 117, 422, 1267
PaeI GCATGC 1 cut(s) 3188
PaeR7I CTCGAG 1 cut(s) 3826
PagI TCATGA 2 cut(s) 3057, 4013
PaqCI CACCTGC 2 cut(s) 944, 1682
PceI AGGCCT 1 cut(s) 2099
PciI ACATGT 1 cut(s) 3217
PciSI GCTCTTC 2 cut(s) 1215, 1269
PctI GAATGC 4 cut(s) 430, 2141, 2654, 2724
PdmI GAANNNNTTC 3 cut(s) 871, 1224, 3638
PfeI GAWTC 9 cut(s) 86, 253, 1238, 1775, 1960, 2501, 2540, 3693, 3808
Pfl23II CGTACG 1 cut(s) 3356
PfoI TCCNGGA 4 cut(s) 576, 2496, 3854, 3966
PinAI ACCGGT 1 cut(s) 3526
PleI GAGTC 6 cut(s) 2440, 2556, 2778, 2865, 3069, 3393
PluTI GGCGCC 1 cut(s) 3020
PmaCI CACGTG 1 cut(s) 2855
PmlI CACGTG 1 cut(s) 2855
PpsI GAGTC 6 cut(s) 2440, 2556, 2778, 2865, 3069, 3393
Ppu21I YACGTR 4 cut(s) 2855, 2911, 3356, 3798
PpuMI RGGWCCY 1 cut(s) 1829
PscI ACATGT 1 cut(s) 3217
PshBI ATTAAT 1 cut(s) 2987
PsiI TTATAA 1 cut(s) 3516
Psp5II RGGWCCY 1 cut(s) 1829
Psp6I CCWGG 9 cut(s) 1504, 1831, 2496, 2753, 3230, 3854, 3903, 3957, 3966
PspCI CACGTG 1 cut(s) 2855
PspGI CCWGG 9 cut(s) 1504, 1831, 2496, 2753, 3230, 3854, 3903, 3957, 3966
PspLI CGTACG 1 cut(s) 3356
PspN4I GGNNCC 8 cut(s) 159, 499, 600, 1107, 1121, 2149, 2577, 3018
PspPI GGNCC 6 cut(s) 1462, 1829, 2345, 2576, 3716, 3820
PspPPI RGGWCCY 1 cut(s) 1829
PspXI VCTCGAGB 1 cut(s) 3826
PstI CTGCAG 3 cut(s) 964, 1693, 3612
PstNI CAGNNNCTG 3 cut(s) 959, 1383, 3251
PsuI RGATCY 5 cut(s) 598, 1380, 1424, 1763, 3541
PvuII CAGCTG 1 cut(s) 3171
RsaI GTAC 4 cut(s) 984, 3216, 3358, 3983
RsaNI GTAC 4 cut(s) 983, 3215, 3357, 3982
RseI CAYNNNNRTG 2 cut(s) 1709, 3796
SapI GCTCTTC 2 cut(s) 1215, 1269
Sau96I GGNCC 6 cut(s) 1462, 1829, 2345, 2576, 3716, 3820
SchI GAGTC 6 cut(s) 2440, 2557, 2779, 2865, 3070, 3393
SduI GDGCHC 5 cut(s) 478, 1264, 2182, 2471, 2860
SfaNI GCATC 9 cut(s) 967, 1318, 1496, 1618, 1984, 2037, 2385, 2611, 3316
SfcI CTRYAG 5 cut(s) 960, 1455, 1689, 2719, 3608
SfoI GGCGCC 1 cut(s) 3018
Sfr274I CTCGAG 1 cut(s) 3826
SfuI TTCGAA 3 cut(s) 117, 422, 1267
SinI GGWCC 4 cut(s) 1462, 1829, 2345, 3716
SlaI CTCGAG 1 cut(s) 3826
SmiMI CAYNNNNRTG 2 cut(s) 1709, 3796
SmlI CTYRAG 5 cut(s) 1352, 2873, 3321, 3460, 3826
SmoI CTYRAG 5 cut(s) 1352, 2873, 3321, 3460, 3826
SpeI ACTAGT 2 cut(s) 491, 659
SphI GCATGC 1 cut(s) 3188
SseBI AGGCCT 1 cut(s) 2099
SspDI GGCGCC 1 cut(s) 3016
SspI AATATT 1 cut(s) 1793
SspMI CTAG 8 cut(s) 72, 492, 549, 660, 1496, 1676, 1929, 3051
StuI AGGCCT 1 cut(s) 2099
StyI CCWWGG 5 cut(s) 1095, 1495, 2348, 2597, 2923
TaaI ACNGT 9 cut(s) 412, 509, 1610, 1909, 2086, 2862, 3630, 3675, 3912
TaiI ACGT 5 cut(s) 2857, 2913, 3358, 3800, 4002
TaqII GACCGA 1 cut(s) 3656
TatI WGTACW 1 cut(s) 3981
TauI GCSGC 5 cut(s) 154, 166, 169, 3821, 3934
TfiI GAWTC 9 cut(s) 86, 253, 1238, 1775, 1960, 2501, 2540, 3693, 3808
TseFI GTSAC 5 cut(s) 2248, 2629, 3137, 3351, 3417
Tsp45I GTSAC 5 cut(s) 2248, 2629, 3137, 3351, 3417
TspGWI ACGGA 4 cut(s) 591, 1785, 2428, 3486
Vha464I CTTAAG 1 cut(s) 3460
VneI GTGCAC 2 cut(s) 474, 2856
VpaK11BI GGWCC 4 cut(s) 1462, 1829, 2345, 3716
VspI ATTAAT 1 cut(s) 2987
XceI RCATGY 3 cut(s) 2299, 3188, 3221
XcmI CCANNNNNNNNNTGG 3 cut(s) 2230, 3081, 3369
XhoI CTCGAG 1 cut(s) 3826
XmaJI CCTAGG 1 cut(s) 1495
XmiI GTMKAC 2 cut(s) 2718, 3670
XmnI GAANNNNTTC 3 cut(s) 871, 1224, 3638
XspI CTAG 8 cut(s) 72, 492, 549, 660, 1496, 1676, 1929, 3051
Zsp2I ATGCAT 3 cut(s) 610, 1999, 3331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.