pycom02g09580

Bifunctional nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0007911
Physical Location & Seq
Reverse (-)
6619065 .. 6621322
2258 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 978 bp
ATGCTCGGAGCCGGAGCTCAATGCCGCGGCCCGGATCGCTTAGCTGCCATCCGTCCGATTCCAAACACTTCTTCTTCTTCCTCCTCCTCTTCGCGCCACTTGGTCTGCACGGTTCGGTTAGGGTTTATCAGATCACCAAAACGGTGCAATTCGCTCAAATCGCTCCGCATTGCCTGCTGCAACTCTTCCTCTCCCTCCAGATCCAGCAAAGCCGACGATCATCACGATTTCGACTACATCCAAGCTTCCCTCCTCCTCTCAGAAACTATATCTCACTACCGTATGCAAAGGCGAGGTTTTCAAGAAGAGACGAAATGGCAGTCATCTGGTAAAATGCGTCCCTTTTCCGTCCGAGCCAGCCGACAACATAATTTTTTCGATACATTAGGCCGAAACTTTGTCCAGCGTTTCCAAAGTCCGACGATTTTTCTCAAGATTTCTTGTGATGGAGACTTTTTGCTTCCAATTGTTGTAGGCGAATTCGCGATCGAAAATCTCATAGACGGCCAATGGGGAGATGAGAATGGGGACTCCCCGGATCAATTTCACTTTATGAGAAATCTCGTTGACAAACTCGGCTATCAAGTGAATATGGTGAGAATTACGGAAAGAGTGCGGAATAATTACTTTGCCAGATTGTACTTTAGCAAGCCAGGGGAAAATGGCTTTCTTAGTGTTGATGCACGCCCATCAGACGCCATAAATTTCGCACATAGATGCAAGGCACCCATATATGTAAACAAACAGATTGTCTTAACAGATGCAGTGAGGATCAGTTATGGGATGGGCGGAGGGCGCGTGACAAAGTCTTGTTATGATGTATCTCTTGACAGCGCTATAGAGGGCCCAGATTCTTTAATTCAAGAACTTGGTTTGGTCAAGAATATCAATCTAGCTATCAAAGAGGAAAGATATAAAGATGCAGCCATATGGAGAGACGAACTATTCAAGCTTCGCAAGTCAATTCAAGGGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNase-RNase UVR

Protein Analysis

326

Amino Acids

36.69

Weight (kDa)

9.34

Isoelectric Point (pI)

47.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BFN_dom PF02577 150 - 256 7.2e-19 Bifunctional nuclease domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 724
AccII CGCG 4 cut(s) 27, 94, 485, 798
AciI CCGC 5 cut(s) 25, 27, 166, 616, 789
AclWI GGATC 4 cut(s) 42, 195, 546, 779
AcoI YGGCCR 1 cut(s) 505
AcsI RAATTY 2 cut(s) 479, 703
AcyI GRCGYC 1 cut(s) 696
AfaI GTAC 1 cut(s) 641
AfeI AGCGCT 1 cut(s) 835
AfiI CCNNNNNNNGG 1 cut(s) 31
AgsI TTSAA 4 cut(s) 302, 863, 949, 968
AjnI CCWGG 1 cut(s) 652
AjuI GAANNNNNNNTTGG 2 cut(s) 55, 87
AluBI AGCT 5 cut(s) 17, 44, 245, 896, 952
AluI AGCT 5 cut(s) 17, 44, 245, 896, 952
Alw21I GWGCWC 1 cut(s) 19
Alw26I GTCTC 3 cut(s) 302, 444, 930
AlwI GGATC 4 cut(s) 42, 195, 546, 779
Aor51HI AGCGCT 1 cut(s) 835
AoxI GGCC 5 cut(s) 28, 388, 505, 844, 971
ApaI GGGCCC 1 cut(s) 848
ApeKI GCWGC 3 cut(s) 44, 177, 923
ApoI RAATTY 2 cut(s) 479, 703
ArsI GACNNNNNNTTYG 4 cut(s) 305, 337, 735, 767
AspLEI GCGC 3 cut(s) 96, 798, 836
AspS9I GGNCC 4 cut(s) 29, 844, 845, 971
AsuC2I CCSGG 2 cut(s) 32, 536
AsuHPI GGTGA 2 cut(s) 126, 607
BaeGI GKGCMC 1 cut(s) 848
BanI GGYRCC 1 cut(s) 724
BanII GRGCYC 2 cut(s) 19, 848
Bbv12I GWGCWC 1 cut(s) 19
BbvI GCAGC 3 cut(s) 31, 164, 935
BccI CCATC 4 cut(s) 56, 440, 697, 778
BceAI ACGGC 1 cut(s) 520
BciT130I CCWGG 1 cut(s) 654
BcnI CCSGG 2 cut(s) 32, 536
BcoDI GTCTC 3 cut(s) 302, 444, 930
BfaI CTAG 1 cut(s) 893
BfmI CTRYAG 1 cut(s) 837
BfoI RGCGCY 1 cut(s) 837
BisI GCNGC 5 cut(s) 25, 28, 45, 178, 924
BlpI GCTNAGC 1 cut(s) 40
BlsI GCNGC 5 cut(s) 26, 29, 46, 179, 925
Bme1390I CCNGG 3 cut(s) 32, 536, 654
BmgT120I GGNCC 4 cut(s) 29, 844, 845, 971
BmiI GGNNCC 3 cut(s) 10, 726, 846
BmrFI CCNGG 3 cut(s) 32, 536, 654
BmsI GCATC 4 cut(s) 670, 707, 751, 910
BpmI CTGGAG 1 cut(s) 181
Bpu1102I GCTNAGC 1 cut(s) 40
BpuEI CTTGAG 1 cut(s) 416
BpuMI CCSGG 2 cut(s) 32, 536
BsaHI GRCGYC 1 cut(s) 696
BsaJI CCNNGG 3 cut(s) 25, 534, 653
BsaXI ACNNNNNCTCC 2 cut(s) 783, 813
Bsc4I CCNNNNNNNGG 1 cut(s) 31
Bse3DI GCAATG 1 cut(s) 168
BseBI CCWGG 1 cut(s) 654
BseDI CCNNGG 3 cut(s) 25, 534, 653
BseGI GGATG 3 cut(s) 48, 237, 789
BseLI CCNNNNNNNGG 1 cut(s) 31
BseMI GCAATG 1 cut(s) 168
BseMII CTCAG 1 cut(s) 273
BseRI GAGGAG 4 cut(s) 73, 76, 242, 245
BseSI GKGCMC 1 cut(s) 848
BseXI GCAGC 3 cut(s) 31, 164, 935
BsgI GTGCAG 1 cut(s) 91
Bsh1236I CGCG 4 cut(s) 27, 94, 485, 798
Bsh1285I CGRYCG 1 cut(s) 489
BshFI GGCC 5 cut(s) 30, 390, 507, 846, 973
BshNI GGYRCC 1 cut(s) 724
BsiEI CGRYCG 1 cut(s) 489
BsiHKAI GWGCWC 1 cut(s) 19
BsiSI CCGG 3 cut(s) 12, 32, 536
BslFI GGGAC 2 cut(s) 324, 542
BslI CCNNNNNNNGG 1 cut(s) 31
BsmAI GTCTC 3 cut(s) 302, 444, 930
BsmBI CGTCTC 2 cut(s) 302, 930
BsmFI GGGAC 2 cut(s) 324, 542
BsnI GGCC 5 cut(s) 30, 390, 507, 846, 973
Bsp120I GGGCCC 1 cut(s) 844
Bsp1286I GDGCHC 2 cut(s) 19, 848
Bsp143I GATC 7 cut(s) 34, 131, 200, 217, 486, 538, 771
Bsp1720I GCTNAGC 1 cut(s) 40
Bsp68I TCGCGA 1 cut(s) 485
BspACI CCGC 5 cut(s) 25, 27, 166, 616, 789
BspANI GGCC 5 cut(s) 30, 390, 507, 846, 973
BspCNI CTCAG 1 cut(s) 272
BspFNI CGCG 4 cut(s) 27, 94, 485, 798
BspLI GGNNCC 3 cut(s) 10, 726, 846
BspPI GGATC 4 cut(s) 42, 195, 546, 779
BspT107I GGYRCC 1 cut(s) 724
BsrDI GCAATG 1 cut(s) 168
BssECI CCNNGG 3 cut(s) 25, 534, 653
BssMI GATC 7 cut(s) 34, 131, 200, 217, 486, 538, 771
BssNI GRCGYC 1 cut(s) 696
Bst2UI CCWGG 1 cut(s) 654
Bst4CI ACNGT 3 cut(s) 112, 144, 281
Bst6I CTCTTC 3 cut(s) 94, 190, 300
BstACI GRCGYC 1 cut(s) 696
BstAPI GCANNNNNTGC 1 cut(s) 174
BstC8I GCNNGC 4 cut(s) 175, 358, 650, 685
BstDEI CTNAG 3 cut(s) 40, 259, 671
BstDSI CCRYGG 1 cut(s) 25
BstF5I GGATG 3 cut(s) 48, 237, 789
BstFNI CGCG 4 cut(s) 27, 94, 485, 798
BstH2I RGCGCY 1 cut(s) 837
BstHHI GCGC 3 cut(s) 96, 798, 836
BstKTI GATC 7 cut(s) 37, 134, 203, 220, 489, 541, 774
BstMAI GTCTC 3 cut(s) 302, 444, 930
BstMBI GATC 7 cut(s) 34, 131, 200, 217, 486, 538, 771
BstMCI CGRYCG 1 cut(s) 489
BstMWI GCNNNNNNNGC 4 cut(s) 36, 160, 174, 795
BstNI CCWGG 1 cut(s) 654
BstSCI CCNGG 3 cut(s) 30, 534, 652
BstSFI CTRYAG 1 cut(s) 837
BstSLI GKGCMC 1 cut(s) 848
BstUI CGCG 4 cut(s) 27, 94, 485, 798
BstV1I GCAGC 3 cut(s) 31, 164, 935
BstX2I RGATCY 1 cut(s) 200
BstYI RGATCY 1 cut(s) 200
BsuRI GGCC 5 cut(s) 30, 390, 507, 846, 973
BtgI CCRYGG 1 cut(s) 25
BtsCI GGATG 3 cut(s) 48, 237, 789
BtsI GCAGTG 1 cut(s) 771
BtsIMutI CAGTG 1 cut(s) 771
BtuMI TCGCGA 1 cut(s) 485
Cac8I GCNNGC 4 cut(s) 175, 358, 650, 685
CfoI GCGC 3 cut(s) 96, 798, 836
Cfr13I GGNCC 4 cut(s) 29, 844, 845, 971
Cfr42I CCGCGG 1 cut(s) 28
CseI GACGC 2 cut(s) 326, 704
Csp6I GTAC 1 cut(s) 640
CviQI GTAC 1 cut(s) 640
DdeI CTNAG 3 cut(s) 40, 259, 671
DpnI GATC 7 cut(s) 36, 133, 202, 219, 488, 540, 773
DpnII GATC 7 cut(s) 34, 131, 200, 217, 486, 538, 771
EaeI YGGCCR 1 cut(s) 505
Eam1104I CTCTTC 3 cut(s) 94, 190, 300
EarI CTCTTC 3 cut(s) 94, 190, 300
EciI GGCGGA 1 cut(s) 804
Ecl136II GAGCTC 1 cut(s) 17
Eco24I GRGCYC 2 cut(s) 19, 848
Eco47III AGCGCT 1 cut(s) 835
Eco53kI GAGCTC 1 cut(s) 17
EcoICRI GAGCTC 1 cut(s) 17
EcoO109I RGGNCCY 2 cut(s) 844, 971
EcoRI GAATTC 1 cut(s) 479
EcoRII CCWGG 1 cut(s) 652
EcoT38I GRGCYC 2 cut(s) 19, 848
Esp3I CGTCTC 2 cut(s) 302, 930
FaqI GGGAC 2 cut(s) 324, 542
FauNDI CATATG 1 cut(s) 929
Fnu4HI GCNGC 5 cut(s) 25, 28, 45, 178, 924
FokI GGATG 3 cut(s) 35, 224, 796
FriOI GRGCYC 2 cut(s) 19, 848
Fsp4HI GCNGC 5 cut(s) 25, 28, 45, 178, 924
FspBI CTAG 1 cut(s) 893
GlaI GCGC 3 cut(s) 95, 797, 835
GluI GCNGC 5 cut(s) 25, 28, 45, 178, 924
GsuI CTGGAG 1 cut(s) 181
HaeII RGCGCY 1 cut(s) 837
HaeIII GGCC 5 cut(s) 30, 390, 507, 846, 973
HapII CCGG 3 cut(s) 12, 32, 536
HgaI GACGC 2 cut(s) 326, 704
HhaI GCGC 3 cut(s) 96, 798, 836
Hin1I GRCGYC 1 cut(s) 696
Hin6I GCGC 3 cut(s) 94, 796, 834
HinP1I GCGC 3 cut(s) 94, 796, 834
HincII GTYRAC 1 cut(s) 568
HindII GTYRAC 1 cut(s) 568
HindIII AAGCTT 2 cut(s) 243, 950
HinfI GANTC 3 cut(s) 58, 530, 851
HpaII CCGG 3 cut(s) 12, 32, 536
HphI GGTGA 2 cut(s) 126, 607
Hpy166II GTNNAC 2 cut(s) 568, 739
Hpy188I TCNGA 7 cut(s) 8, 57, 131, 262, 353, 420, 694
Hpy188III TCNNGA 8 cut(s) 198, 224, 302, 433, 484, 827, 863, 880
Hpy8I GTNNAC 2 cut(s) 568, 739
Hpy99I CGWCG 2 cut(s) 218, 424
HpyCH4III ACNGT 3 cut(s) 112, 144, 281
HpyCH4V TGCA 8 cut(s) 108, 147, 180, 286, 683, 720, 764, 923
HpyF10VI GCNNNNNNNGC 4 cut(s) 36, 160, 174, 795
HpyF3I CTNAG 3 cut(s) 40, 259, 671
Hsp92I GRCGYC 1 cut(s) 696
HspAI GCGC 3 cut(s) 94, 796, 834
KspI CCGCGG 1 cut(s) 28
Kzo9I GATC 7 cut(s) 34, 131, 200, 217, 486, 538, 771
LmnI GCTCC 3 cut(s) 8, 14, 168
Lsp1109I GCAGC 3 cut(s) 31, 164, 935
LweI GCATC 4 cut(s) 670, 707, 751, 910
MaeI CTAG 1 cut(s) 893
MaeIII GTNAC 1 cut(s) 799
MalI GATC 7 cut(s) 36, 133, 202, 219, 488, 540, 773
MboI GATC 7 cut(s) 34, 131, 200, 217, 486, 538, 771
MboII GAAGA 6 cut(s) 63, 66, 69, 81, 177, 317
MfeI CAATTG 1 cut(s) 465
MflI RGATCY 1 cut(s) 200
MhlI GDGCHC 2 cut(s) 19, 848
MlyI GAGTC 1 cut(s) 524
MmeI TCCRAC 1 cut(s) 443
MseI TTAA 2 cut(s) 755, 857
MslI CAYNNNNRTG 1 cut(s) 715
MspA1I CMGCKG 1 cut(s) 27
MspI CCGG 3 cut(s) 12, 32, 536
MspR9I CCNGG 3 cut(s) 32, 536, 654
MunI CAATTG 1 cut(s) 465
MvaI CCWGG 1 cut(s) 654
MvnI CGCG 4 cut(s) 27, 94, 485, 798
MwoI GCNNNNNNNGC 4 cut(s) 36, 160, 174, 795
NciI CCSGG 2 cut(s) 32, 536
NdeI CATATG 1 cut(s) 929
NdeII GATC 7 cut(s) 34, 131, 200, 217, 486, 538, 771
NlaIV GGNNCC 3 cut(s) 10, 726, 846
NmeAIII GCCGAG 1 cut(s) 555
NmuCI GTSAC 1 cut(s) 799
NruI TCGCGA 1 cut(s) 485
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PfeI GAWTC 2 cut(s) 58, 851
PflFI GACNNNGTC 1 cut(s) 805
PkrI GCNGC 5 cut(s) 26, 29, 46, 179, 925
Ple19I CGATCG 1 cut(s) 489
PleI GAGTC 1 cut(s) 524
PpsI GAGTC 1 cut(s) 524
Psp124BI GAGCTC 1 cut(s) 19
Psp6I CCWGG 1 cut(s) 652
PspGI CCWGG 1 cut(s) 652
PspN4I GGNNCC 3 cut(s) 10, 726, 846
PspOMI GGGCCC 1 cut(s) 844
PspPI GGNCC 4 cut(s) 29, 844, 845, 971
PsuI RGATCY 1 cut(s) 200
PsyI GACNNNGTC 1 cut(s) 805
PvuI CGATCG 1 cut(s) 489
RruI TCGCGA 1 cut(s) 485
RsaI GTAC 1 cut(s) 641
RsaNI GTAC 1 cut(s) 640
RseI CAYNNNNRTG 1 cut(s) 715
SacI GAGCTC 1 cut(s) 19
SacII CCGCGG 1 cut(s) 28
SaqAI TTAA 2 cut(s) 755, 857
SatI GCNGC 5 cut(s) 25, 28, 45, 178, 924
Sau3AI GATC 7 cut(s) 34, 131, 200, 217, 486, 538, 771
Sau96I GGNCC 4 cut(s) 29, 844, 845, 971
SchI GAGTC 1 cut(s) 524
ScrFI CCNGG 3 cut(s) 32, 536, 654
SduI GDGCHC 2 cut(s) 19, 848
SetI ASST 6 cut(s) 19, 46, 247, 298, 898, 954
SfaNI GCATC 4 cut(s) 670, 707, 751, 910
SfcI CTRYAG 1 cut(s) 837
Sfr303I CCGCGG 1 cut(s) 28
SgrBI CCGCGG 1 cut(s) 28
SmiMI CAYNNNNRTG 1 cut(s) 715
SmlI CTYRAG 1 cut(s) 431
SmoI CTYRAG 1 cut(s) 431
SsiI CCGC 5 cut(s) 25, 27, 166, 616, 789
SspMI CTAG 1 cut(s) 893
SstI GAGCTC 1 cut(s) 19
StyD4I CCNGG 3 cut(s) 30, 534, 652
TaaI ACNGT 3 cut(s) 112, 144, 281
TaqI TCGA 3 cut(s) 231, 378, 489
TatI WGTACW 1 cut(s) 639
TauI GCSGC 2 cut(s) 27, 30
TfiI GAWTC 2 cut(s) 58, 851
Tru1I TTAA 2 cut(s) 755, 857
Tru9I TTAA 2 cut(s) 755, 857
TscAI CASTG 1 cut(s) 771
TseFI GTSAC 1 cut(s) 799
TseI GCWGC 3 cut(s) 44, 177, 923
Tsp45I GTSAC 1 cut(s) 799
TspGWI ACGGA 3 cut(s) 41, 337, 620
TspRI CASTG 1 cut(s) 771
Tth111I GACNNNGTC 1 cut(s) 805
XapI RAATTY 2 cut(s) 479, 703
XspI CTAG 1 cut(s) 893
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.