pycom02g14610

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0002655
Physical Location & Seq
Forward (+)
11200587 .. 11201346
760 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 552 bp
ATGTTTCATTTTCTTGTTGATGCTTTGGTAGAATGGTGCATCACACTTACTTTTCTAGCGCGTTTGATTGCGTACATTGCAATTTTAAGTAAGATTTCTTTCATCAACCAAATATGTACTGCAGCATTGCTTGTGATTGTTGCATTCATGGTAATGAAGTACATAAACGAATATAAGGAGACCGGCTTAGAGGATGTAGGAGGCAGTGCTGAAACTGACCCGTTACAGCCTGCAGGAAAGACAATGCGATCAACATATGGGACATGTGAAGAGTGGGATATTGAATCCGGAAAGTGCCGCAGCAGCAGTAGTAATAGCAACAATGCCGTAATTATGAGCTCCTCAGAGGACTTGTATGACGGGAAAATATGTGCGATCTGCTACACTGAGCAGAGGGATTGTTTTTTGGTTCCTTGTGGCCACTGTGCTACCTGCTATGTCTGTGCAGAAAGAGATTACATGTTGCAGGATCTTTTACGGGGAGACCAAGACATGTCCAATATGTCGAAGATTCATACGGAAAGTAAGAAGATTATTTGCTGCATAGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4792 DUF4793 zf-C3HC4_3

Protein Analysis

184

Amino Acids

20.42

Weight (kDa)

4.82

Isoelectric Point (pI)

47.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 122 - 151 1.6e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 440
AccII CGCG 1 cut(s) 61
AccIII TCCGGA 1 cut(s) 287
AciI CCGC 1 cut(s) 298
AclWI GGATC 1 cut(s) 477
AcoI YGGCCR 1 cut(s) 418
AfaI GTAC 3 cut(s) 74, 118, 161
AflIII ACRYGT 3 cut(s) 263, 459, 492
AgsI TTSAA 1 cut(s) 284
AluBI AGCT 1 cut(s) 339
AluI AGCT 1 cut(s) 339
Alw21I GWGCWC 1 cut(s) 341
Alw26I GTCTC 2 cut(s) 173, 477
AlwI GGATC 1 cut(s) 477
Aor13HI TCCGGA 1 cut(s) 287
AoxI GGCC 1 cut(s) 418
ApeKI GCWGC 4 cut(s) 122, 300, 303, 540
AspLEI GCGC 1 cut(s) 61
BalI TGGCCA 1 cut(s) 420
BanII GRGCYC 1 cut(s) 341
Bbv12I GWGCWC 1 cut(s) 341
BbvI GCAGC 4 cut(s) 134, 312, 315, 527
BceAI ACGGC 1 cut(s) 311
BcoDI GTCTC 2 cut(s) 173, 477
BfaI CTAG 1 cut(s) 56
BfmI CTRYAG 2 cut(s) 120, 231
BfuAI ACCTGC 1 cut(s) 440
BisI GCNGC 5 cut(s) 123, 298, 301, 304, 541
BlsI GCNGC 5 cut(s) 124, 299, 302, 305, 542
BmiI GGNNCC 1 cut(s) 411
BmsI GCATC 2 cut(s) 10, 48
BsaI GGTCTC 2 cut(s) 173, 477
BsaWI WCCGGW 1 cut(s) 287
Bse118I RCCGGY 1 cut(s) 182
Bse3DI GCAATG 2 cut(s) 75, 125
BseAI TCCGGA 1 cut(s) 287
BseGI GGATG 1 cut(s) 199
BseMI GCAATG 2 cut(s) 75, 125
BseMII CTCAG 2 cut(s) 357, 378
BseRI GAGGAG 1 cut(s) 331
BseXI GCAGC 4 cut(s) 134, 312, 315, 527
BsgI GTGCAG 1 cut(s) 465
Bsh1236I CGCG 1 cut(s) 61
BshFI GGCC 1 cut(s) 420
BsiHKAI GWGCWC 1 cut(s) 341
BsiSI CCGG 2 cut(s) 183, 288
BslFI GGGAC 1 cut(s) 274
BsmAI GTCTC 2 cut(s) 173, 477
BsmFI GGGAC 1 cut(s) 274
BsmI GAATGC 1 cut(s) 143
BsnI GGCC 1 cut(s) 420
Bso31I GGTCTC 2 cut(s) 173, 477
Bsp1286I GDGCHC 1 cut(s) 341
Bsp13I TCCGGA 1 cut(s) 287
Bsp143I GATC 3 cut(s) 248, 375, 469
BspACI CCGC 1 cut(s) 298
BspANI GGCC 1 cut(s) 420
BspCNI CTCAG 2 cut(s) 356, 379
BspEI TCCGGA 1 cut(s) 287
BspFNI CGCG 1 cut(s) 61
BspLI GGNNCC 1 cut(s) 411
BspMAI CTGCAG 2 cut(s) 124, 235
BspMI ACCTGC 1 cut(s) 440
BspPI GGATC 1 cut(s) 477
BspTNI GGTCTC 2 cut(s) 173, 477
BsrDI GCAATG 2 cut(s) 75, 125
BsrFI RCCGGY 1 cut(s) 182
BssAI RCCGGY 1 cut(s) 182
BssMI GATC 3 cut(s) 248, 375, 469
Bst4CI ACNGT 1 cut(s) 425
Bst6I CTCTTC 1 cut(s) 264
BstC8I GCNNGC 1 cut(s) 231
BstDEI CTNAG 3 cut(s) 187, 343, 387
BstF5I GGATG 1 cut(s) 199
BstFNI CGCG 1 cut(s) 61
BstHHI GCGC 1 cut(s) 61
BstKTI GATC 3 cut(s) 251, 378, 472
BstMAI GTCTC 2 cut(s) 173, 477
BstMBI GATC 3 cut(s) 248, 375, 469
BstMWI GCNNNNNNNGC 2 cut(s) 77, 303
BstNSI RCATGY 3 cut(s) 267, 463, 496
BstSFI CTRYAG 2 cut(s) 120, 231
BstUI CGCG 1 cut(s) 61
BstV1I GCAGC 4 cut(s) 134, 312, 315, 527
BstX2I RGATCY 1 cut(s) 469
BstYI RGATCY 1 cut(s) 469
BsuRI GGCC 1 cut(s) 420
BtsCI GGATG 1 cut(s) 199
BtsI GCAGTG 1 cut(s) 211
BtsIMutI CAGTG 3 cut(s) 211, 384, 421
BveI ACCTGC 1 cut(s) 440
Cac8I GCNNGC 1 cut(s) 231
CfoI GCGC 1 cut(s) 61
Cfr10I RCCGGY 1 cut(s) 182
Csp6I GTAC 3 cut(s) 73, 117, 160
CviAII CATG 4 cut(s) 148, 264, 460, 493
CviJI RGCY 4 cut(s) 186, 229, 339, 420
CviKI_1 RGCY 4 cut(s) 186, 229, 339, 420
CviQI GTAC 3 cut(s) 73, 117, 160
DdeI CTNAG 3 cut(s) 187, 343, 387
DpnI GATC 3 cut(s) 250, 377, 471
DpnII GATC 3 cut(s) 248, 375, 469
EaeI YGGCCR 1 cut(s) 418
Eam1104I CTCTTC 1 cut(s) 264
EarI CTCTTC 1 cut(s) 264
Ecl136II GAGCTC 1 cut(s) 339
Eco24I GRGCYC 1 cut(s) 341
Eco31I GGTCTC 2 cut(s) 173, 477
Eco53kI GAGCTC 1 cut(s) 339
EcoICRI GAGCTC 1 cut(s) 339
EcoT38I GRGCYC 1 cut(s) 341
FaeI CATG 4 cut(s) 151, 267, 463, 496
FaqI GGGAC 1 cut(s) 274
FatI CATG 4 cut(s) 147, 263, 459, 492
FauNDI CATATG 1 cut(s) 256
Fnu4HI GCNGC 5 cut(s) 123, 298, 301, 304, 541
FokI GGATG 1 cut(s) 206
FriOI GRGCYC 1 cut(s) 341
Fsp4HI GCNGC 5 cut(s) 123, 298, 301, 304, 541
FspBI CTAG 1 cut(s) 56
GlaI GCGC 1 cut(s) 60
GluI GCNGC 5 cut(s) 123, 298, 301, 304, 541
HaeIII GGCC 1 cut(s) 420
HapII CCGG 2 cut(s) 183, 288
HhaI GCGC 1 cut(s) 61
Hin1II CATG 4 cut(s) 151, 267, 463, 496
Hin6I GCGC 1 cut(s) 59
HinP1I GCGC 1 cut(s) 59
HinfI GANTC 2 cut(s) 284, 511
HpaII CCGG 2 cut(s) 183, 288
Hpy188I TCNGA 1 cut(s) 346
Hpy188III TCNNGA 1 cut(s) 288
HpyCH4III ACNGT 1 cut(s) 425
HpyCH4V TGCA 8 cut(s) 39, 80, 122, 143, 233, 446, 466, 543
HpyF10VI GCNNNNNNNGC 2 cut(s) 77, 303
HpyF3I CTNAG 3 cut(s) 187, 343, 387
Hsp92II CATG 4 cut(s) 151, 267, 463, 496
HspAI GCGC 1 cut(s) 59
Kpn2I TCCGGA 1 cut(s) 287
Kzo9I GATC 3 cut(s) 248, 375, 469
LmnI GCTCC 1 cut(s) 344
LpnPI CCDG 6 cut(s) 196, 219, 243, 301, 445, 452
Lsp1109I GCAGC 4 cut(s) 134, 312, 315, 527
LweI GCATC 2 cut(s) 10, 48
MaeI CTAG 1 cut(s) 56
MaeIII GTNAC 1 cut(s) 222
MalI GATC 3 cut(s) 250, 377, 471
MboI GATC 3 cut(s) 248, 375, 469
MboII GAAGA 3 cut(s) 281, 520, 541
MflI RGATCY 1 cut(s) 469
MhlI GDGCHC 1 cut(s) 341
MlsI TGGCCA 1 cut(s) 420
MluCI AATT 2 cut(s) 81, 330
MluNI TGGCCA 1 cut(s) 420
MnlI CCTC 5 cut(s) 184, 194, 340, 352, 387
Mox20I TGGCCA 1 cut(s) 420
MroI TCCGGA 1 cut(s) 287
MscI TGGCCA 1 cut(s) 420
MseI TTAA 1 cut(s) 86
MslI CAYNNNNRTG 1 cut(s) 152
Msp20I TGGCCA 1 cut(s) 420
MspI CCGG 2 cut(s) 183, 288
Mva1269I GAATGC 1 cut(s) 143
MvnI CGCG 1 cut(s) 61
MwoI GCNNNNNNNGC 2 cut(s) 77, 303
NdeI CATATG 1 cut(s) 256
NdeII GATC 3 cut(s) 248, 375, 469
NlaIII CATG 4 cut(s) 151, 267, 463, 496
NlaIV GGNNCC 1 cut(s) 411
NspI RCATGY 3 cut(s) 267, 463, 496
PciI ACATGT 3 cut(s) 263, 459, 492
PctI GAATGC 1 cut(s) 143
PfeI GAWTC 2 cut(s) 284, 511
PkrI GCNGC 5 cut(s) 124, 299, 302, 305, 542
PscI ACATGT 3 cut(s) 263, 459, 492
Psp124BI GAGCTC 1 cut(s) 341
PspN4I GGNNCC 1 cut(s) 411
PstI CTGCAG 2 cut(s) 124, 235
PsuI RGATCY 1 cut(s) 469
RsaI GTAC 3 cut(s) 74, 118, 161
RsaNI GTAC 3 cut(s) 73, 117, 160
RseI CAYNNNNRTG 1 cut(s) 152
SacI GAGCTC 1 cut(s) 341
SaqAI TTAA 1 cut(s) 86
SatI GCNGC 5 cut(s) 123, 298, 301, 304, 541
Sau3AI GATC 3 cut(s) 248, 375, 469
SbfI CCTGCAGG 1 cut(s) 235
SdaI CCTGCAGG 1 cut(s) 235
SduI GDGCHC 1 cut(s) 341
SetI ASST 2 cut(s) 341, 434
SfaNI GCATC 2 cut(s) 10, 48
SfcI CTRYAG 2 cut(s) 120, 231
SmiMI CAYNNNNRTG 1 cut(s) 152
Sse8387I CCTGCAGG 1 cut(s) 235
Sse9I AATT 2 cut(s) 81, 330
SsiI CCGC 1 cut(s) 298
SspMI CTAG 1 cut(s) 56
SstI GAGCTC 1 cut(s) 341
TaaI ACNGT 1 cut(s) 425
TaqI TCGA 1 cut(s) 506
TasI AATT 2 cut(s) 81, 330
TatI WGTACW 2 cut(s) 116, 159
TauI GCSGC 1 cut(s) 300
TfiI GAWTC 2 cut(s) 284, 511
Tru1I TTAA 1 cut(s) 86
Tru9I TTAA 1 cut(s) 86
TscAI CASTG 3 cut(s) 211, 391, 428
TseI GCWGC 4 cut(s) 122, 300, 303, 540
TspDTI ATGAA 4 cut(s) 91, 136, 170, 503
TspGWI ACGGA 1 cut(s) 533
TspRI CASTG 3 cut(s) 211, 391, 428
XceI RCATGY 3 cut(s) 267, 463, 496
XspI CTAG 1 cut(s) 56
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.